Patents by Inventor Yuiko Sahara

Yuiko Sahara has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Calcium-binding photoprotein, gene encoding the same, and use thereof
    Patent number: 8563270
    Abstract: A protein according to the invention can be used to detect or measure calcium ions is provided. Further the protein is useful as a reporter protein or a luminescence marker. A polynucleotide according to the invention is also useful as a reporter gene.
    Type: Grant
    Filed: June 20, 2012
    Date of Patent: October 22, 2013
    Assignee: JNC Corporation
    Inventors: Satoshi Inouye, Yuiko Sahara
  • Coelenterazine analogs and manufacturing method thereof
    Patent number: 8546568
    Abstract: There has been a need for coelenterazine analogs that exhibit luminescence properties different from those of known coelenterazine analogs. The present invention provides the compound represented by general formula (1).
    Type: Grant
    Filed: February 8, 2010
    Date of Patent: October 1, 2013
    Assignees: JNC Corporation, Tokyo Institute of Technology
    Inventors: Satoshi Inouye, Yuiko Sahara, Rie Iimori, Takamitsu Hosoya
  • FUSION PROTEIN HAVING LUMINESCENCE ACTIVITY
    Publication number: 20130252256
    Abstract: The fusion protein comprising (1) a first region comprising the amino acid sequence of SEQ ID NO: 18 and (2) a second region comprising an amino acid sequence for a polypeptide containing at least one cysteine residue for binding to other useful compound via the thiol group can be modified by chemical modification, and thus has a high catalytic ability for a luminescence activity and is highly available for general purposes.
    Type: Application
    Filed: June 3, 2013
    Publication date: September 26, 2013
    Applicant: JNC CORPORATION
    Inventors: Satoshi INOUYE, Yuiko SAHARA, Junichi SATO
  • Fusion protein having luminescence activity
    Patent number: 8481702
    Abstract: The fusion protein comprising (1) a first region comprising the amino acid sequence of SEQ ID NO: 18 and (2) a second region comprising an amino acid sequence for a polypeptide containing at least one cysteine residue for binding to other useful compound via the thiol group can be modified by chemical modification, and thus has a high catalytic ability for a luminescence activity and is highly available for general purposes.
    Type: Grant
    Filed: February 8, 2013
    Date of Patent: July 9, 2013
    Assignee: JNC Corporation
    Inventors: Satoshi Inouye, Yuiko Sahara, Junichi Sato
  • Method for enhancing luminescence intensity of clytin-II
    Patent number: 8476436
    Abstract: A method for enhancing a luminescence activity of clytin-II is provided. A codon-optimized nucleic acid is used for coding the apo-clytin-II protein, and the luminescent activity of the clytin-II is remarkably enhanced when comparing with the conventional use of the wild-type clytin-II.
    Type: Grant
    Filed: February 7, 2013
    Date of Patent: July 2, 2013
    Assignee: JNC Corporation
    Inventors: Satoshi Inouye, Yuiko Sahara, Junichi Sato
  • Method for determining capability of a compound for inhibiting a receptor using codon-optimized nucleic acid
    Patent number: 8476038
    Abstract: A method for determining a capability of a compound for inhibiting a receptor through a change of intracellular calcium ion when the receptor is activated is provided. Contacting a host cell genetically engineered to express an apo-clytin-II protein generated from a codon-optimized nucleic acid with a test compound, and then determining an amount of light generated. The intracellular calcium ion can be detected much more easily.
    Type: Grant
    Filed: February 7, 2013
    Date of Patent: July 2, 2013
    Assignee: JNC Corporation
    Inventors: Satoshi Inouye, Yuiko Sahara, Junichi Sato
  • FUSION PROTEIN HAVING LUMINESCENCE ACTIVITY
    Publication number: 20130143233
    Abstract: The fusion protein comprising (1) a first region comprising the amino acid sequence of SEQ ID NO: 18 and (2) a second region comprising an amino acid sequence for a polypeptide containing at least one cysteine residue for binding to other useful compound via the thiol group can be modified by chemical modification, and thus has a high catalytic ability for a luminescence activity and is highly available for general purposes.
    Type: Application
    Filed: February 8, 2013
    Publication date: June 6, 2013
    Applicant: JNC CORPORATION
    Inventors: Satoshi INOUYE, Yuiko SAHARA, Junichi SATO
  • Fusion protein having luminescence activity
    Patent number: 8399616
    Abstract: The fusion protein comprising (1) a first region comprising the amino acid sequence of SEQ ID NO: 18 and (2) a second region comprising an amino acid sequence for a polypeptide containing at least one cysteine residue for binding to other useful compound via the thiol group can be modified by chemical modification, and thus has a high catalytic ability for a luminescence activity and is highly available for general purposes.
    Type: Grant
    Filed: December 22, 2010
    Date of Patent: March 19, 2013
    Assignee: JNC Corporation
    Inventors: Satoshi Inouye, Yuiko Sahara, Junichi Sato
  • Codon-optimized nucleic acid for coding apo-clytin-II and method for using the same
    Patent number: 8394603
    Abstract: A codon-optimization of nucleic acid for coding apo-clytin-II protein is provided. Luminescent activity of clytin-II is remarkably enhanced. Accordingly, compared with the conventional use of the wild-type clytin-II, an intracellular calcium ion can be detected much more easily.
    Type: Grant
    Filed: January 27, 2010
    Date of Patent: March 12, 2013
    Assignee: JNC Corporation
    Inventors: Satoshi Inouye, Yuiko Sahara, Junichi Sato
  • CALCIUM-BINDING PHOTOPROTEIN, GENE ENCODING THE SAME, AND USE THEREOF
    Publication number: 20120258486
    Abstract: A protein according to the invention can be used to detect or measure calcium ions is provided. Further the protein is useful as a reporter protein or a luminescence marker. A polynucleotide according to the invention is also useful as a reporter gene.
    Type: Application
    Filed: June 20, 2012
    Publication date: October 11, 2012
    Applicant: JNC CORPORATION
    Inventors: Satoshi INOUYE, Yuiko SAHARA
  • Calcium-binding photoprotein, gene encoding the same, and use thereof
    Patent number: 8236526
    Abstract: A protein according to the invention can be used to detect or measure calcium ions is provided. Further the protein is useful as a reporter protein or a luminescence marker. A polynucleotide according to the invention is also useful as a reporter gene.
    Type: Grant
    Filed: December 22, 2009
    Date of Patent: August 7, 2012
    Assignee: JNC Corporation
    Inventors: Satoshi Inouye, Yuiko Sahara
  • COELENTERAZINE ANALOGS AND MANUFACTURING METHOD THEREOF
    Publication number: 20120035070
    Abstract: There has been a need for coelenterazine analogs that exhibit luminescence properties different from those of known coelenterazine analogs. The present invention provides the compound represented by general formula (1).
    Type: Application
    Filed: February 8, 2010
    Publication date: February 9, 2012
    Applicants: Tokyo Institute of Technology, JNC Corporation
    Inventors: Satoshi Inouye, Yuiko Sahara, Rie Iimori, Takamitsu Hosoya
  • COELENTERAZINE ANALOGUES AND COELENTERAMIDE ANALOGUES
    Publication number: 20110244481
    Abstract: Coelenterazine analogues with different luminescence properties from conventional ones and coelenteramide analogues with different fluorescence properties from conventional ones have been desired. The invention provides coelenterazine analogues modified at the 8-position of coelenterazine and coelenteramide analogues modified at the 2- or 3-position of coelenteramide.
    Type: Application
    Filed: April 5, 2011
    Publication date: October 6, 2011
    Applicants: JNC CORPORATION, NATIONAL UNIVERSITY CORPORATION TOKYO MEDICAL AND DENTAL UNIVERSITY
    Inventors: SATOSHI INOUYE, YUIKO SAHARA, TAKAMITSU HOSOYA
  • FUSION PROTEIN HAVING LUMINESCENCE ACTIVITY
    Publication number: 20110159529
    Abstract: The fusion protein comprising (1) a first region comprising the amino acid sequence of SEQ ID NO: 18 and (2) a second region comprising an amino acid sequence for a polypeptide containing at least one cysteine residue for binding to other useful compound via the thiol group can be modified by chemical modification, and thus has a high catalytic ability for a luminescence activity and is highly available for general purposes.
    Type: Application
    Filed: December 22, 2010
    Publication date: June 30, 2011
    Applicant: CHISSO CORPORATION
    Inventors: Satoshi INOUYE, Yuiko SAHARA, Junichi SATO
  • Fusion protein of Fc-binding domain and calcium-binding photoprotein, gene encoding the same and use thereof
    Patent number: 7888087
    Abstract: The invention provides a calcium-binding photoprotein which has a high ability to bind to IgG and is capable of more sensitively detecting IgG. A fusion protein of the present invention composed of a ZZ domain having the ability to bind to the Fc region of IgG and the calcium-binding photoprotein apoaequorin is used for the detection and assay of calcium ions and the detection of IgG.
    Type: Grant
    Filed: August 21, 2007
    Date of Patent: February 15, 2011
    Assignee: Chisso Corporation
    Inventors: Satoshi Inouye, Yuiko Sahara, Junichi Sato
  • Calcium-binding photoprotein, gene encoding the same and use thereof
    Publication number: 20100311051
    Abstract: The invention provides calcium-binding photoproteins which can detect light emission with a higher sensitivity. The proteins of the invention comprising the amino acid sequence of SEQ ID NO: 2 can be used for the detection and measurement of calcium ions. The proteins of the invention are useful as reporter proteins, luminescent markers, etc. The polynucleotides of the invention are useful as reporter genes, etc.
    Type: Application
    Filed: May 10, 2010
    Publication date: December 9, 2010
    Inventors: Satoshi Inouye, Yuiko Sahara
  • CODON-OPTIMIZED NUCLEIC ACID FOR CODING APO-CLYTIN-II AND METHOD FOR USING THE SAME
    Publication number: 20100273202
    Abstract: A codon-optimization of nucleic acid for coding apo-clytin-II protein is provided. Luminescent activity of clytin-II is remarkably enhanced. Accordingly, compared with the conventional use of the wild-type clytin-II, an intracellular calcium ion can be detected much more easily.
    Type: Application
    Filed: January 27, 2010
    Publication date: October 28, 2010
    Applicant: CHISSO CORPORATION
    Inventors: Satoshi Inouye, Yuiko Sahara, Junichi Sato
  • Calcium-Binding Photoprotein, Gene Encoding the Same, and Use Thereof
    Publication number: 20100203585
    Abstract: A protein according to the invention can be used to detect or measure calcium ions is provided. Further the protein is useful as a reporter protein or a luminescence marker. A polynucleotide according to the invention is also useful as a reporter gene.
    Type: Application
    Filed: December 22, 2009
    Publication date: August 12, 2010
    Applicant: CHISSO CORPORATION
    Inventors: Satoshi INOUYE, Yuiko SAHARA
  • Calcium-binding photoprotein, gene encoding the same, and use thereof
    Patent number: 7666980
    Abstract: A protein according to the invention can be used to detect or measure calcium ions is provided. Further the protein is useful as a reporter protein or a luminescence marker. A polynucleotide according to the invention is also useful as a reporter gene.
    Type: Grant
    Filed: July 19, 2007
    Date of Patent: February 23, 2010
    Assignee: Chisso Corporation
    Inventors: Satoshi Inouye, Yuiko Sahara
  • Calcium-binding photoprotein, gene encoding the same, and use thereof
    Publication number: 20090011460
    Abstract: A protein according to the invention can be used to detect or measure calcium ions is provided. Further the protein is useful as a reporter protein or a luminescence marker. A polynucleotide according to the invention is also useful as a reporter gene.
    Type: Application
    Filed: July 19, 2007
    Publication date: January 8, 2009
    Inventors: Satoshi Inouye, Yuiko Sahara