Patents by Inventor Yuji Maruo
Yuji Maruo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9103781Abstract: A sample separation and adsorption appliance (100) includes a negative electrode (2), a positive electrode (3), a sample separation unit (6) that has a first opening (17) opened to a side facing the negative electrode (2) and a second opening (18) opened to a side facing the positive electrode (3), the sample separation unit containing a separation gel (7), and a slit structure (8) including a slit (1) at a position facing the second opening (18). A transfer film (9) is arranged between the second opening (18) and the slit (1).Type: GrantFiled: September 12, 2011Date of Patent: August 11, 2015Assignee: Sharp Kabushiki KaishaInventors: Kouji Kusumoto, Yutaka Unuma, Hideki Kinoshita, Shinichi Goto, Tsuyoshi Tanaka, Yuji Maruo
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Publication number: 20150160156Abstract: To easily introduce a sample into a sample separation medium without formation of a well in the sample separation medium, a cassette (100) for electrophoresis includes a porous member (132) containing an unseparated sample, a sample separation medium (112) for separating the sample, and a pushing tool (131) that presses the porous member (132) or the sample separation medium (112) so that the porous member (132) is pushed into the sample separation medium (112).Type: ApplicationFiled: June 4, 2013Publication date: June 11, 2015Inventors: Mieko Otonashi, Hideki Kinoshita, Yutaka Unuma, Yuji Maruo
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Publication number: 20150160158Abstract: There is provided an electrophoresis instrument capable of easily performing a separation of a biological sample by isoelectric focusing with excellent reproducibility. An electrophoresis instrument (10) includes an electrophoresis chamber (1) in which a sample separation medium (42) for separating the biological sample by electrophoresis is installed. A depression (2) for injecting a sample solution that includes the biological sample is provided on an installation surface (1a) on which the sample separation medium (42) of the electrophoresis chamber (1) is installed.Type: ApplicationFiled: June 27, 2013Publication date: June 11, 2015Inventors: Uichi Midorikawa, Hideki Kinoshita, Yutaka Unuma, Yuji Maruo
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Publication number: 20150014168Abstract: An electrophoresis control device, on the basis of a current value of current flowing due to applying a voltage to a conducting medium, controls an amount of electric power to supply to that conducting medium.Type: ApplicationFiled: December 21, 2012Publication date: January 15, 2015Inventors: Hideki Kinoshita, Yutaka Unuma, Yuji Maruo
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Publication number: 20150010867Abstract: An electrophoresis gel, in which a good pH gradient or concentration gradient of a gel-forming monomer is formed, is produced. In addition, the production efficiency of an electrophoresis reaction tool is improved and the production processes are simplified. At least one of a first process to add a gel-forming monomer to a first solution containing an initiator to initiate polymerization of the gel-forming monomer by external energy in such a way that a pH gradient or concentration gradient of the gel-forming monomer is formed and a second process to initiate the polymerization of the above-described gel-forming monomer in the first solution, to which the above-described gel-forming monomer has been added, by using the above-described external energy is included.Type: ApplicationFiled: February 26, 2013Publication date: January 8, 2015Applicant: Sharp Kabushiki KaishaInventors: Hiroshi Ohki, Hiroshi Yamaki, Tsuyoshi Tanaka, Yoshiyuki Ishida, Yutaka Unuma, Yuji Maruo
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Publication number: 20140374260Abstract: With intent to increase the number of sample spots separated through two-dimensional electrophoresis and to enhance the intensity in detection of the spots, a two-dimensional electrophoresis kit includes a first medium 7 for first dimensional electrophoresis, a second medium 8 for second dimensional electrophoresis, and a casing 20 that contains at least the first medium 7 and the second medium 8, wherein the first medium 7 is formed by supplying, to the casing 20, a first solution containing a sample on which the first dimensional electrophoresis is to be performed, and the first medium 7 and the second medium 8 are contained close to each other.Type: ApplicationFiled: February 6, 2013Publication date: December 25, 2014Applicant: Sharp Kabushiki KaishaInventors: Hiroshi Ohki, Tsuyoshi Tanaka, Hiroshi Yamaki, Yoshiyuki Ishida, Yutaka Unuma, Yuji Maruo
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Publication number: 20140224657Abstract: A sample separation and adsorption appliance (100) includes a negative electrode (2), a positive electrode (3), a sample separation unit (6) that has a first opening (17) opened to a side facing the negative electrode (2) and a second opening (18) opened to a side facing the positive electrode (3), the sample separation unit containing a separation gel (7), and a slit structure (8) including a slit (1) at a position facing the second opening (18). A transfer film (9) is arranged between the second opening (18) and the slit (1).Type: ApplicationFiled: September 12, 2011Publication date: August 14, 2014Applicant: SHARP KABUSHIKI KAISHAInventors: Kouji Kusumoto, Yutaka Unuma, Hideki Kinoshita, Shinichi Goto, Tsuyoshi Tanaka, Yuji Maruo
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Publication number: 20140110261Abstract: A chip (10) for electrophoresis is provided, the chip including a gel (9) composed of a polymer prepared by polymerizing a monomer, and a support (1) configured to support the gel (9). In the chip (10) for electrophoresis, a surface of the support (1) in contact with the gel (9) is covered with a surface treatment compound (4) containing the monomer or a derivative of the monomer.Type: ApplicationFiled: June 19, 2012Publication date: April 24, 2014Applicant: SHARP KABUSHIKI KAISHAInventors: Hiroshi Ohki, Yuji Maruo, Yutaka Unuma
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Patent number: 8702950Abstract: A device for electrophoresis applies a voltage to a medium in contact with a plurality of electric conductors so that a potential of adjacent conductors is within a certain range. This allows preventing decline in electrophoresis speed. A device for electrophoresis and transfer includes an electrode having a plurality of electrode regions being insulated one another and arranged in a specific direction. This allows providing a practical and easy-to-use device for electrophoresis and transfer. A device for transfer alters an applied voltage or applied voltage duration to a certain position to another position. This allows improving transfer efficiency.Type: GrantFiled: September 10, 2012Date of Patent: April 22, 2014Assignees: Sharp Kabushiki Kaisha, Toppan Printing Co., Ltd.Inventors: Yuji Maruo, Yutaka Unuma, Atsunori Hiratsuka, Hideki Kinoshita, Kenji Yokoyama, Koji Sakairi, Satonari Akutsu
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Publication number: 20140076727Abstract: A solution ejecting method includes a first ejection step of ejecting an electrophoresis-gel-forming monomer solution containing at least a monomer that forms a gel structure and a gel polymerization accelerator that activates a gel polymerization initiator and a second ejection step of ejecting a gel polymerization initiating solution containing the gel polymerization initiator onto the electrophoresis-gel-forming monomer solution.Type: ApplicationFiled: March 27, 2012Publication date: March 20, 2014Inventors: Hiroshi Ohki, Hiroshi Yamaki, Yutaka Unuma, Yuji Maruo, Tsuyoshi Tanaka
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Patent number: 8500981Abstract: An electrophoresis apparatus includes a sample separating section for containing a sample separating medium for separating a sample in a horizontal direction, the sample separating section containing the sample separating medium in such a manner that the sample separating medium has an exposed portion at least one end of a surface of the sample separating medium, the surface being in parallel with the horizontal direction; and medium connecting means for connecting a sample containing medium to the sample separating medium at a connecting region, the sample containing medium containing a sample, and the connecting region satisfying a particular equation. By this, an electrophoresis technique with better accuracy than conventional arts can be provided.Type: GrantFiled: July 15, 2009Date of Patent: August 6, 2013Assignees: Toppan Printing Co., Ltd., Sharp Kabushiki KaishaInventors: Koji Sakairi, Satonari Akutsu, Yuji Maruo, Takateru Matsunaga, Yutaka Unuma, Atsunori Hiratsuka, Hideki Kinoshita, Nao Sakaguchi, Kenji Yokoyama
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Patent number: 8449748Abstract: A sample separation/adsorption appliance (100) according to one embodiment of the present invention includes: a first buffer solution tank (103) equipped with a first electrode (101) and used for holding a first buffer solution; a second buffer solution tank (104) equipped with a second electrode (102) and used for holding a second buffer solution; a sample separation section (110) that holds a sample separation medium (131) for separating a sample; and a conductive section (120) that holds a conductive medium (133), wherein: the sample separation medium (131) and the conductive medium (133) are in contact with the sample adsorption member (132), respectively, on the opposite sides thereof; an end of the sample separation medium (131) opposite to an end being in contact with the sample adsorption member (132) is connected to an inside of the first buffer solution tank (103); and an end of the conductive medium (133) opposite to an end being in contact with the sample adsorption member (132) is connected to anType: GrantFiled: June 1, 2009Date of Patent: May 28, 2013Assignees: Sharp Kabushiki Kaisha, National Institute of Advanced Industrial Science and TechnologyInventors: Uichi Midorikawa, Takateru Matsunaga, Yuji Maruo, Yutaka Unuma, Atsunori Hiratsuka, Hideki Kinoshita, Kenji Yokoyama
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Publication number: 20130098764Abstract: A production method of the present invention is a method of producing a reaction instrument for electrophoresis (10), which is constituted by (i) a substrate (1) and (ii) an electrophoresis gel (3) which is immobilized on the substrate (1), including the steps of: (i) discharging a liquid to a surface of the substrate (1) on which surface the gel is to be immobilized, thereby forming a liquid pool (5); and thereafter (ii) discharging a gel solution to the liquid pool (5). This provides a method of producing a reaction instrument for electrophoresis, which method makes it possible to form a gel with high reliability in high yield.Type: ApplicationFiled: January 12, 2011Publication date: April 25, 2013Applicant: SHARP KABUSHIKI KAISHAInventors: Hiroshi Ohki, Yutaka Unuma, Yuji Maruo, Tsuyoshi Tanaka, Hiroshi Yamaki
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Patent number: 8419916Abstract: A biological molecule separation apparatus for separating a biological molecule of one embodiment of the present invention has a buffer solution chamber receiving a buffer solution, a separation electrode arranged in the buffer solution chamber, a biological molecule separation medium for separating a biological molecule, a biological molecule adsorption film for adsorbing a biological molecule, a pair of transfer electrodes having a plurality of line shaped conductors extending in a direction perpendicular to a first direction, the conductors being arranged in the direction perpendicular to the first direction, and a separation part which holds the transfer electrode, the biological molecule adsorption film, the medium and the transfer electrode, wherein the transfer electrode, the biological molecule adsorption film, the medium and the transfer electrode are stacked in this order.Type: GrantFiled: March 28, 2011Date of Patent: April 16, 2013Assignee: Toppan Printing Co., Ltd.Inventors: Koji Sakairi, Satonari Akutsu, Atsunori Hiratsuka, Kenji Yokoyama, Yuji Maruo, Hiroshi Ohki
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Publication number: 20130015069Abstract: A device for electrophoresis applies a voltage to a medium in contact with a plurality of electric conductors so that a potential of adjacent conductors is within a certain range. This allows preventing decline in electrophoresis speed. A device for electrophoresis and transfer includes an electrode having a plurality of electrode regions being insulated one another and arranged in a specific direction. This allows providing a practical and easy-to-use device for electrophoresis and transfer. A device for transfer alters an applied voltage or applied voltage duration to a certain position to another position. This allows improving transfer efficiency.Type: ApplicationFiled: September 10, 2012Publication date: January 17, 2013Applicant: Sharp Kabushiki Kaisha and Toppan Printing Co., Ltd.Inventors: Yuji MARUO, Yutaka Unuma, Atsunori Hiratsuka, Hideki Kinoshita, Kenji Yokoyama, Koji Sakairi, Satonari Akutsu
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Publication number: 20120273360Abstract: An instrument for electrophoresis includes a sample-separating portion in which a slab sample-separating medium that separates a sample is provided. The instrument for electrophoresis includes a sample-transporting portion that transports a sample-containing medium containing the sample to the sample-separating portion. The instrument for electrophoresis includes a positioning portion that is provided to the sample-separating portion and/or the sample-transporting portion and used for connecting the sample-transporting portion to a predetermined position of the sample-separating portion.Type: ApplicationFiled: December 21, 2010Publication date: November 1, 2012Inventors: Hideki Kinoshita, Yuji Maruo, Yutaka Unuma, Atsunori Hiratsuka, Koji Sakairi, Satonari Akutsu
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Publication number: 20110233062Abstract: A biological molecule separation apparatus for separating a biological molecule of one embodiment of the present invention has a buffer solution chamber receiving a buffer solution, a separation electrode arranged in the buffer solution chamber, a biological molecule separation medium for separating a biological molecule, a biological molecule adsorption film for adsorbing a biological molecule, a pair of transfer electrodes having a plurality of line shaped conductors extending in a direction perpendicular to a first direction, the conductors being arranged in the direction perpendicular to the first direction, and a separation part which holds the transfer electrode, the biological molecule adsorption film, the medium and the transfer electrode, wherein the transfer electrode, the biological molecule adsorption film, the medium and the transfer electrode are stacked in this order.Type: ApplicationFiled: March 28, 2011Publication date: September 29, 2011Applicant: Toppan Printing Co., Ltd.Inventors: Koji Sakairi, Satonari Akutsu, Atsunori Hiratsuka, Kenji Yokoyama, Yuji Maruo, Hiroshi Ohki
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Patent number: 7964077Abstract: The present invention is to provide a sample separation instrument used in a sample separation apparatus which includes: holding means for holding a first medium supporter which supports a first medium; and driving means for moving fixing means or the holding means in a direction parallel or perpendicular to a plane whose sides extend in the first direction and in the second directions. The sample separation instrument includes an insulator for storing a second medium which allows a sample separated in the first medium in the first direction to be further separated in the second direction different from the first direction, wherein: the insulator includes a first opening and a second opening each of which defines the second direction in which the second medium is electrified, and the second opening has a shape which allows the first medium containing the separated sample to be attached to the second medium along the first direction.Type: GrantFiled: August 28, 2006Date of Patent: June 21, 2011Assignees: Sharp Kabushiki Kaisha, Toppan Printing Co., LtdInventors: Yuji Maruo, Katsuyoshi Takahashi, Yutaka Unuma, Keisuke Usui, Hideki Kinoshita, Kisho Shiseki, Yoshio Suzuki, Atsunori Hiratsuka, Hiroyuki Fukui, Kenji Yokoyama, Ichiji Namatame, Kouhei Yodoya, Yasuhiro Ogawa, Koji Sakairi, Chie Hayashida, Satonari Akutsu, Kazuyoshi Yano, Isao Karube
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Patent number: 7951279Abstract: There is provided an electrophoresis device including an insulator that includes: a first-separating-medium storing section for storing therein a first separating medium; a first opening and a second opening that are in communication with the first-separating-medium storing section and for defining a direction of separation on the first separating medium; and a third opening that is covered with a penetrable insulating thin film, wherein the first separating medium storing section is in communication with outside via the first opening and the second opening. There is also provided an electrophoresis apparatus including the electrophoresis device. This realizes an electrophoresis apparatus, and a device therefor, that enables an operator to sample separated proteins without ever making contact with the electrophoresed gel, and that can easily be used with various types of analyses, thereby improving convenience of electrophoresis.Type: GrantFiled: September 5, 2006Date of Patent: May 31, 2011Assignees: Toppan Printing Co., Ltd., Sharp Kabushiki KaishaInventors: Chie Hayashida, Koji Sakairi, Katsuyoshi Takahashi, Yuji Maruo, Michinobu Mieda, Yutaka Unuma
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Publication number: 20110114485Abstract: An electrophoresis apparatus includes a sample separating section for containing a sample separating medium for separating a sample in a horizontal direction, the sample separating section containing the sample separating medium in such a manner that the sample separating medium has an exposed portion at least one end of a surface of the sample separating medium, the surface being in parallel with the horizontal direction; and medium connecting means for connecting a sample containing medium to the sample separating medium at a connecting region, the sample containing medium containing a sample, and the connecting region satisfying a particular equation. By this, an electrophoresis technique with better accuracy than conventional arts can be provided.Type: ApplicationFiled: July 15, 2009Publication date: May 19, 2011Inventors: Koji Sakairi, Satonari Akutsu, Yuji Maruo, Takateru Matsunaga, Yutaka Unuma, Atsunori Hiratsuka, Hideki Kinoshita, Nao Sakaguchi, Kenji Yokoyama