Abstract: A point management system using a vehicle as a contact point includes: an evaluation device that evaluates a specific activity of an user of the vehicle based on vehicle information according to a travel state of the vehicle or activity information recorded in a smart device of the user; a point giving device that gives the point to the user according to an evaluation by the evaluation device; and a propose device that proposes, to the user, an activity for which the point is given. The specific activity includes at least one of three categories of an eco-driving activity, a health care activity, and a social contribution activity.

Abstract: A semiconductor device includes: a substrate; a buffer layer; an intermediate layer; an electron transport layer; an electron supply layer; a source electrode and a drain electrode; and a gate electrode. The intermediate layer includes a stack resulting from stacking a first intermediate layer and a second intermediate layer. The second intermediate layer is provided above the first intermediate layer. A first position that is 100 nm above a lower surface of the intermediate layer is in the first intermediate layer. A second position that is 100 nm below an upper surface of the intermediate layer is in the second intermediate layer. A value obtained by dividing a density of edge screw mixed dislocations with a Burgers vector of <11-23>/3 at the second position by a density of edge screw mixed dislocations with the Burgers vector of <11-23>/3 at the first position is at most 0.66.

Abstract: An information processing device includes a first communication unit, a second communication unit, an information processing unit, and a switching unit. The information processing unit is configured to encrypt information which is received from a terminal device and to transmit the encrypted information to a network and configured to decrypt information which is received from the network and to transmit the decrypted information to the terminal device. The information processing device includes a switching unit configured to directly connect a communication line between the first communication unit and the terminal device to another communication line between the second communication unit and the network, when the information processing unit comes into an inoperable state including at least electric power supply stop state, and to switch into a pass-through mode in which the terminal device and the network communicate directly with each other without through the information processing unit.

Abstract: A method for designing a protein capable of binding in an RNA base selective manner or RNA base sequence specific manner is provided.

Abstract: It was found that four nuclease domain mutants have nuclease activity superior to that of a wild-type nuclease domain and are capable of enhancing genome editing efficiency in combination with various nucleic acid binding domains.

Abstract: The present invention provides a polyamide and a polyamide film which are more sufficiently superior in all of heat-resisting properties, flexibility properties and rubber elastic properties. The present invention relates to a polyamide comprising a unit comprised of an aliphatic dicarboxylic acid (A) having 18 or more carbon atoms, a unit comprised of an aliphatic diamine (B) having 18 or more carbon atoms, a unit comprised of an aromatic dicarboxylic acid (C) having 12 or less carbon atoms and a unit comprised of an aliphatic diamine (D) having 12 or less carbon atoms, wherein the polyamide has a melting point of 240° C. or more, a crystalline melting enthalpy of 20 J/g or more and an elongation recovery rate of 50% or more in a hysteresis test, and a polyamide film comprising the polyamide.

Abstract: A method for designing a protein capable of binding in an RNA base selective manner or RNA base sequence specific manner is provided.

Abstract: A method for converting an editing target C contained in a target RNA to U or an editing target U contained in a target RNA to C is provided. A method for editing a target RNA, which comprises applying an artificial DYW protein containing a DYW domain consisting of any one of the polypeptides a, b, c, and be mentioned below to the target RNA: a. a polypeptide having xa1PGxa1SWIExa3-xa16HP . . . HxaaE . . . Cxa17Xa18CH . . . DYW, having a sequence identity of at least 40% to the sequence of SEQ ID NO: 1, and having a C-to-U/U-to-C editing activity, b. a polypeptide having xb1PGxb2SWWTDxb3-xb16HP . . . HxbbE . . . Cxb17xb18CH . . . DYW, having a sequence identity of at least 40% to the sequence of SEQ ID NO: 2, and having a C-to-U/U-to-C editing activity, c. a polypeptide having KPAxc1Axc2IExc3 . . . HxccE . . . Cxc4xc5CH . . . xc6xc7xc8, having a sequence identity of at least 40% to the sequence of SEQ ID NO: 3, and having a C-to-U/U-to-C editing activity, and be. a polypeptide having xb1PGxb2SWWTDxb3-xb16HP .

Abstract: An object is to provide a metal-resin bonded member that is easy to manufacture and has high bonding strength. The metal-resin bonded member includes a metal body having an iron oxide layer on the surface and a resin body bonded to the metal body via the iron oxide layer. The iron oxide layer has a thickness of 50 nm to 10 ?m. The iron oxide layer comprises 60-40 at % Fe and 40-60 at % O at the outermost surface side. The iron oxide layer contains magnetite (Fe3O4). The iron oxide layer is formed by heating the surface of an iron-based substrate at 200-850° C. in an oxidation atmosphere. The resin body is composed of polyphenylene sulfide (PPS). The bonding of the metal body and the resin body via the iron oxide layer can be carried out by insert molding, thermal adhesion utilizing friction heating, etc.

Abstract: A flexible polyamide film obtained by forming a polyamide including a unit formed from an aliphatic dicarboxylic acid (A1) having 18 or more carbon atoms and/or a unit formed from an aliphatic diamine (B1) having 18 or more carbon atoms, wherein the polyamide has a total content of 10 to 92% by mass of the unit formed from (A1) and the unit formed from (B1), and the film has a melting point of 240° C. or higher.

Abstract: A PPR protein with high performance is provided. A PPR protein that binds to a long nucleotide sequence is provided by linking motifs in a number larger than conventionally used 7 to 14. A PPR motif is provided, of which typical examples are the followings: (A-1) a PPR motif consisting of the sequence of SEQ ID NO: 9 or 401, (C-1) a PPR motif consisting of the sequence of SEQ ID NO: 10, (G-1) a PPR motif consisting of the sequence of SEQ ID NO: 11, and (U-1) a PPR motif consisting of the sequence of SEQ ID NO: 12. These motifs are useful as PPR motifs for adenine, cytosine, guanine, and uracil in a target nucleotide sequence, respectively.

Abstract: In order to improve aggregation property of a PPR protein, the A6 amino acid of the 1st PPR motif from the N-terminus (M1) is made more hydrophilic. Further, the A9 amino acid of M1 is made to be a hydrophilic amino acid or glycine. The A6 amino acid is preferably asparagine or aspartic acid, and the A9 amino acid is preferably glutamine, glutamic acid, lysine, or glycine. Proteins containing such a PPR motif as M1 motif may have not only improved aggregation property, but also high binding power to a target nucleic acid.

Abstract: A polyamide resin composition including 100 parts by mass of a polyamide (A), 3.0 to 10.0 parts by mass of a polyalcohol (B), and 0.0 to 4.0 parts by mass of an aromatic polycarboxylic acid (C), wherein the total amount of a carboxyl group of (A) and (C) is 80 to 600 mmol per 1 kg in total of (A), (B), and (C).

Abstract: A method for designing a protein capable of binding in a DNA base selective manner or DNA base sequence specific manner is provided. According to the present invention, it was revealed that, with a protein that can bind in a DNA base-selective manner or a DNA base sequence-specific manner, which contains one or more, preferably 2 to 30, more preferably 5 to 25, most preferably 9 to 15, of PPR motifs having a structure of the following formula 1 (wherein, in the formula 1, Helix A is a part that can form an ?-helix structure; X does not exist, or is a part consisting of 1 to 9 amino acids; Helix B is a part that can form an ?-helix structure; and L is a part consisting of 2 to 7 amino acids), and having a specific combination of amino acids corresponding to a DNA base or DNA base sequence as amino acids of three positions of No. 1 A.A., No. 4 A.A., in Helix A of the formula 1 and No. “ii” (-2) A.A. contained in L of the formula 1, the aforementioned object could be achieved.

Abstract: A flexible polyamide including a unit formed from an aliphatic dicarboxylic acid (A1) having 18 or more carbon atoms and/or a unit formed from an aliphatic diamine (B1) having 18 or more carbon atoms, wherein the polyamide has a total content of 10 to 90% by mass of the unit formed from (A1) and the unit formed from (B1) and has a melting point of 240° C. or higher.

Abstract: The object of the present invention is to, by analyzing PPR proteins that act to bind to DNA with a prediction that RNA recognition rules of PPR motifs can also be used for recognition of DNA, find a PPR protein showing such a characteristic. According to the present invention, it was revealed that, with a protein that can bind in a DNA base-selective manner or a DNA base sequence-specific manner, which contains one or more, preferably 2 to 30, more preferably 5 to 25, most preferably 9 to 15, of PPR motifs having a structure of the following formula 1 (wherein, in the formula 1, Helix A is a part that can form an ?-helix structure; X does not exist, or is a part consisting of 1 to 9 amino acids; Helix B is a part that can form an ?-helix structure; and L is a part consisting of 2 to 7 amino acids), and having a specific combination of amino acids corresponding to a DNA base or DNA base sequence as amino acids of three positions of No. 1 A.A., No. 4 A.A., in Helix A of the formula 1 and No. “ii” (-2) A.A.

Abstract: [Problem to be Solved] The object of the present invention is to develop a method of regulating a target RNA. [Solution] There is provided a fusion protein comprising a functional domain which improves the protein expression level from mRNA and a PPR protein which can bind to a target mRNA in an RNA base-selective or RNA base sequence-specific manner.

Abstract: An information processing device includes a first communication unit, a second communication unit, an information processing unit, and a switching unit. The first communication unit is configured to communicate with a terminal device. The second communication unit is configured to communicate with a device connected to a network. The information processing unit is configured to encrypt information which is received from the terminal device by the first communication unit and to transmit the encrypted information to the network via the second communication unit, and is configured to decrypt information which is received from the network by the second communication unit and to transmit the decrypted information to the terminal device via the first communication unit.

Abstract: A method for designing a protein capable of binding in an RNA base selective manner or RNA base sequence specific manner is provided.

Abstract: Disclosed is a method for forming a multilayer coating film, including applying a first and second aqueous base coat, and a clear coat to an object, and thereafter simultaneously heating and curing the three-layer coating film. The first aqueous base coat includes a water-soluble or water-dispersible polyurethane resin having a glass transition temperature of ?20° C. or below and a weight average molecular weight of 30,000 to 500,000, and a crosslinkable resin having a weight average molecular weight of 600 or more. The first aqueous base coat-coated film has a breaking strength of 2050 N/cm2 or more and a loss tangent (tan ?) at ?20° C. of 0.075 or more. The migration amount of a melamine resin from the second aqueous base coat-coated film layer to the first aqueous base coat-coated film layer is within 3% by mass of a total resin solid content of the first aqueous base coat.