Patents by Inventor Zhan-Bin Liu

Zhan-Bin Liu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11913035
    Abstract: Provided are compositions comprising polynucleotides encoding modified diacylglycerol acyltransferase-1 (DGAT1) polypeptides having improved properties, such as increased enzymatic activity and/or increased stability. Plants, plant cells, seed, grain and comprising the polynucleotides are provided which have one or more of increased fatty acid or protein content. Methods of generating the polynucleotides in plant cells include transformation and genetic modification. Methods of employing the polynucleotides in plants, methods for increasing DGAT1 activity in a plant, and methods for increasing fatty acid content or protein content in a plant are provided.
    Type: Grant
    Filed: September 19, 2022
    Date of Patent: February 27, 2024
    Assignee: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: Kayla S Flyckt, Kristin Haug Collet, Zhan-Bin Liu, Keith R Roesler, Bo Shen, Laura L Wayne
  • Publication number: 20230380373
    Abstract: Soybean plants producing soybean seeds comprising leghemoglobin are produced by modifying the genome of the soybean plant. Soybean plants, soybean seeds and soy protein compositions comprising leghemoglobin are provided. Soybean plants, soybean seeds and soy protein compositions comprising leghemoglobin and additionally one or more of high oleic acid, low linolenic acid, high protein, low stachyose, low raffinose and low protease inhibitors are provided. Protein compositions comprising leghemoglobin, such as soy isolates and concentrates can be made from the soybean seeds. Additionally, methods for generating and using plants, seeds and protein compositions comprising leghemoglobin are disclosed.
    Type: Application
    Filed: October 22, 2021
    Publication date: November 30, 2023
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: HYEON-JE CHO, JOHN D. EVERARD, ANTHONY KINNEY, ZHAN-BIN LIU, KNUT MEYER, THOMAS G. PATTERSON, KEVIN G. RIPP, BO SHEN
  • Publication number: 20230323374
    Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed.
    Type: Application
    Filed: November 15, 2022
    Publication date: October 12, 2023
    Applicants: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC
    Inventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, ZHONGSEN LI, ZHAN-BIN LIU, L. ALEKSANDER LYZNIK, JINRUI SHI, SERGEI SVITASHEV, JOSHUA K. YOUNG
  • Patent number: 11773400
    Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell, without incorporating a selectable transgene marker. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed, without incorporating a selectable transgene marker. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a guide polynucleotide/Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell, without incorporating a selectable transgene marker.
    Type: Grant
    Filed: August 20, 2014
    Date of Patent: October 3, 2023
    Inventors: Andrew Mark Cigan, Saverio Carl Falco, Michael Lassner, Zhan-Bin Liu, Sergei Svitashev
  • Publication number: 20230279413
    Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed.
    Type: Application
    Filed: November 16, 2022
    Publication date: September 7, 2023
    Applicants: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC
    Inventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, ZHONGSEN LI, ZHAN-BIN LIU, L. ALEKSANDER LYZNIK, JINRUI SHI, SERGEI SVITASHEV, JOSHUA K. YOUNG
  • Publication number: 20230235342
    Abstract: Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.
    Type: Application
    Filed: August 23, 2022
    Publication date: July 27, 2023
    Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E. I. DU PONT DE NEMOURS AND COMPANY
    Inventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, MICHAEL LASSNER, DEREK JANTZ, ZHONGSEN LI, ZHAN-BIN LIU, SERGEI SVITASHEV, JAMES JEFFERSON SMITH
  • Publication number: 20230235345
    Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed.
    Type: Application
    Filed: November 17, 2022
    Publication date: July 27, 2023
    Applicants: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC
    Inventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, ZHONGSEN LI, ZHAN-BIN LIU, L. ALEKSANDER LYZNIK, JINRUI SHI, SERGEI SVITASHEV, JOSHUA K. YOUNG
  • Publication number: 20230232763
    Abstract: Soybean plants and soybean seeds comprise increased amounts of essential amino acids such as methionine, tryptophan or lysine. Soybean plants, soybean seeds and soy protein compositions comprising a modified glycinin protein, modified amounts of glycinin and beta-conglycinin, or both have increases in one or more essential amino acids. Protein compositions having increased essential amino acids can be made from the soybean seeds. Additionally, methods for generating and using plants, seeds and protein compositions having increased amounts of essential amino acids are disclosed.
    Type: Application
    Filed: April 20, 2021
    Publication date: July 27, 2023
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: ZHENGLIN HOU, ZHAN-BIN LIU, KRISHNA M. MADDURI, BO SHEN, JUN ZHANG
  • Publication number: 20230212595
    Abstract: Compositions and methods are provided for introducing transgenic target sites for Site Specific Integration (SSI) and/or polynucleotides of interest into at least one double-strand break target site of a double-strand-break inducing agent in a genomic window of a plant genome. Also provided are methods and compositions for producing a complex trait locus in a genomic window of a plant comprising at least one transgenic target site for site specific integration integrated in at least double-strand-break target site. The double-strand-break target site can be, but is not limited to, a target site for a zinc finger endonuclease, an engineered endonuclease, a meganuclease, a TALENs and/or a Cas endonuclease. The genomic window of said plant can comprise at least one genomic locus of interest such as a trait cassette, a transgene, a mutated gene, a native gene, an edited gene or a site-specific integration (SSI) target site.
    Type: Application
    Filed: December 15, 2022
    Publication date: July 6, 2023
    Applicants: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: ANDREW MARK CIGAN, HUIRONG GAO, ZHAN-BIN LIU, JASDEEP MUTTI, DEAN PODLICH, CHRISTOPHER SCELONGE
  • Publication number: 20230193304
    Abstract: The disclosure relates to gene expression regulatory sequences from soybean, specifically to the promoter of a soybean U6 polymerase III gene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, mutated plants, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.
    Type: Application
    Filed: July 22, 2022
    Publication date: June 22, 2023
    Applicant: E. I. DU PONT DE NEMOURS AND COMPANY
    Inventors: ZHONGSEN LI, ZHAN-BIN LIU
  • Publication number: 20230151338
    Abstract: Provided are compositions comprising polynucleotides encoding modified diacylglycerol acyltransferase-1 (DGAT1) polypeptides having improved properties, such as increased enzymatic activity and/or increased stability. Plants, plant cells, seed, grain and comprising the polynucleotides are provided which have one or more of increased fatty acid or protein content. Methods of generating the polynucleotides in plant cells include transformation and genetic modification. Methods of employing the polynucleotides in plants, methods for increasing DGAT1 activity in a plant, and methods for increasing fatty acid content or protein content in a plant are provided.
    Type: Application
    Filed: September 19, 2022
    Publication date: May 18, 2023
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: KAYLA S FLYCKT, KRISTIN HAUG COLLET, ZHAN-BIN LIU, KEITH R ROESLER, BO SHEN, LAURA L WAYNE
  • Patent number: 11560568
    Abstract: Compositions and methods are provided for introducing transgenic target sites for Site Specific Integration (SSI) and/or polynucleotides of interest into at least one double-strand break target site of a double-strand-break inducing agent in a genomic window of a plant genome. Also provided are methods and compositions for producing a complex trait locus in a genomic window of a plant comprising at least one transgenic target site for site specific integration integrated in at least double-strand-break target site. The double-strand-break target site can be, but is not limited to, a target site for a zinc finger endonuclease, an engineered endonuclease, a meganuclease, a TALENs and/or a Cas endonuclease. The genomic window of said plant can comprise at least one genomic locus of interest such as a trait cassette, a transgene, a mutated gene, a native gene, an edited gene or a site-specific integration (SSI) target site.
    Type: Grant
    Filed: August 31, 2015
    Date of Patent: January 24, 2023
    Inventors: Andrew Mark Cigan, Huirong Gao, Zhan-Bin Liu, Jasdeep Mutti, Dean Podlich, Christopher Jay Scelonge
  • Publication number: 20220340923
    Abstract: Soybean plants producing soybean seeds comprising leghemoglobin are produced by modifying the genome of the soybean plant. Soybean plants, soybean seeds and soy protein compositions comprising leghemoglobin are provided. Soybean plants, soybean seeds and soy protein compositions comprising leghemoglobin and additionally one or more of high oleic acid, low linolenic acid, high protein, low stachyose, low raffinose and low protease inhibitors are provided. Protein compositions comprising leghemoglobin, such as soy isolates and concentrates can be made from the soybean seeds. Additionally, methods for generating and using plants, seeds and protein compositions comprising leghemoglobin are disclosed.
    Type: Application
    Filed: June 30, 2022
    Publication date: October 27, 2022
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: HYEON-JE CHO, JOHN D EVERARD, ANTHONY J. KINNEY, ZHAN-BIN LIU, KNUT MEYER, THOMAS G PATTERSON, KEVIN G RIPP, BO SHEN
  • Patent number: 11479759
    Abstract: Provided are compositions comprising polynucleotides encoding modified diacylglycerol acyltransferase-1 (DGAT1) polypeptides having improved properties, such as increased enzymatic activity and/or increased stability. Plants, plant cells, seed, grain and comprising the polynucleotides are provided which have one or more of increased fatty acid or protein content. Methods of generating the polynucleotides in plant cells include transformation and genetic modification. Methods of employing the polynucleotides in plants, methods for increasing DGAT1 activity in a plant, and methods for increasing fatty acid content or protein content in a plant are provided.
    Type: Grant
    Filed: May 30, 2019
    Date of Patent: October 25, 2022
    Inventors: Kayla S Flyckt, Kristin Haug Collet, Zhan-Bin Liu, Keith R Roesler, Bo Shen, Laura L Wayne
  • Patent number: 11459576
    Abstract: Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.
    Type: Grant
    Filed: July 10, 2020
    Date of Patent: October 4, 2022
    Inventors: Andrew Mark Cigan, Saverio Carl Falco, Huirong Gao, Michael Lassner, Derek Jantz, Zhongsen Li, Zhan-Bin Liu, Sergei Svitashev, James Jefferson Smith
  • Patent number: 11427830
    Abstract: The disclosure relates to gene expression regulatory sequences, specifically to the promoter of a U6 polymerase III gene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, mutated plants, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.
    Type: Grant
    Filed: August 27, 2019
    Date of Patent: August 30, 2022
    Assignee: E. I. DU PONT DE NEMOURS AND COMPANY
    Inventors: Zhongsen Li, Zhan-Bin Liu
  • Patent number: 11390876
    Abstract: Methods and compositions to modify the fatty acid profiles of seeds and the oils produced therefrom include the modification of four or more alleles of fatty acid desaturases in the genome of a plant. Compositions, polynucleotide constructs, transformed and modified host cells, and plants and seeds exhibiting altered fatty characteristics are provided.
    Type: Grant
    Filed: March 1, 2019
    Date of Patent: July 19, 2022
    Inventors: John D Everard, Anthony J Kinney, Zhan-Bin Liu
  • Patent number: 11359206
    Abstract: Soybean plants producing soybean seeds comprising leghemoglobin are produced by modifying the genome of the soybean plant. Soybean plants, soybean seeds and soy protein compositions comprising leghemoglobin are provided. Soybean plants, soybean seeds and soy protein compositions comprising leghemoglobin and additionally one or more of high oleic acid, low linolenic acid, high protein, low stachyose, low raffinose and low protease inhibitors are provided. Protein compositions comprising leghemoglobin, such as soy isolates and concentrates can be made from the soybean seeds. Additionally, methods for generating and using plants, seeds and protein compositions comprising leghemoglobin are disclosed.
    Type: Grant
    Filed: December 21, 2021
    Date of Patent: June 14, 2022
    Inventors: Hyeon-Je Cho, John D Everard, Anthony J Kinney, Zhan-Bin Liu, Knut Meyer, Thomas G Patterson, Kevin G Ripp, Bo Shen
  • Publication number: 20220127631
    Abstract: Soybean plants producing soybean seeds comprising leghemoglobin are produced by modifying the genome of the soybean plant. Soybean plants, soybean seeds and soy protein compositions comprising leghemoglobin are provided. Soybean plants, soybean seeds and soy protein compositions comprising leghemoglobin and additionally one or more of high oleic acid, low linolenic acid, high protein, low stachyose, low raffinose and low protease inhibitors are provided. Protein compositions comprising leghemoglobin, such as soy isolates and concentrates can be made from the soybean seeds. Additionally, methods for generating and using plants, seeds and protein compositions comprising leghemoglobin are disclosed.
    Type: Application
    Filed: October 22, 2021
    Publication date: April 28, 2022
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: HYEON-JE CHO, JOHN D EVERARD, ANTHONY J KINNEY, ZHAN-BIN LIU, KNUT MEYER, THOMAS G PATTERSON, KEVIN G RIPP, BO SHEN
  • Publication number: 20220127632
    Abstract: Soybean plants producing soybean seeds comprising leghemoglobin are produced by modifying the genome of the soybean plant. Soybean plants, soybean seeds and soy protein compositions comprising leghemoglobin are provided. Soybean plants, soybean seeds and soy protein compositions comprising leghemoglobin and additionally one or more of high oleic acid, low linolenic acid, high protein, low stachyose, low raffinose and low protease inhibitors are provided. Protein compositions comprising leghemoglobin, such as soy isolates and concentrates can be made from the soybean seeds. Additionally, methods for generating and using plants, seeds and protein compositions comprising leghemoglobin are disclosed.
    Type: Application
    Filed: December 21, 2021
    Publication date: April 28, 2022
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: HYEON-JE CHO, JOHN D EVERARD, ANTHONY J KINNEY, ZHAN-BIN LIU, KNUT MEYER, THOMAS G PATTERSON, KEVIN G RIPP, BO SHEN