Patents by Inventor Zhan-Bin Liu
Zhan-Bin Liu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220127631Abstract: Soybean plants producing soybean seeds comprising leghemoglobin are produced by modifying the genome of the soybean plant. Soybean plants, soybean seeds and soy protein compositions comprising leghemoglobin are provided. Soybean plants, soybean seeds and soy protein compositions comprising leghemoglobin and additionally one or more of high oleic acid, low linolenic acid, high protein, low stachyose, low raffinose and low protease inhibitors are provided. Protein compositions comprising leghemoglobin, such as soy isolates and concentrates can be made from the soybean seeds. Additionally, methods for generating and using plants, seeds and protein compositions comprising leghemoglobin are disclosed.Type: ApplicationFiled: October 22, 2021Publication date: April 28, 2022Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: HYEON-JE CHO, JOHN D EVERARD, ANTHONY J KINNEY, ZHAN-BIN LIU, KNUT MEYER, THOMAS G PATTERSON, KEVIN G RIPP, BO SHEN
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Publication number: 20220127632Abstract: Soybean plants producing soybean seeds comprising leghemoglobin are produced by modifying the genome of the soybean plant. Soybean plants, soybean seeds and soy protein compositions comprising leghemoglobin are provided. Soybean plants, soybean seeds and soy protein compositions comprising leghemoglobin and additionally one or more of high oleic acid, low linolenic acid, high protein, low stachyose, low raffinose and low protease inhibitors are provided. Protein compositions comprising leghemoglobin, such as soy isolates and concentrates can be made from the soybean seeds. Additionally, methods for generating and using plants, seeds and protein compositions comprising leghemoglobin are disclosed.Type: ApplicationFiled: December 21, 2021Publication date: April 28, 2022Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: HYEON-JE CHO, JOHN D EVERARD, ANTHONY J KINNEY, ZHAN-BIN LIU, KNUT MEYER, THOMAS G PATTERSON, KEVIN G RIPP, BO SHEN
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Publication number: 20220119827Abstract: Soybean seeds with increased protein or oil and having a modified CCT-domain protein or modified expression of a CCT-domain protein are provided. Methods for modifying expression of CCT-domain polypeptides and polynucleotides include genome editing to modify the transcription regulatory region or sequence encoding the CCT-domain polypeptide and transformation with recombinant DNA constructs to enhance or suppress expression.Type: ApplicationFiled: October 30, 2019Publication date: April 21, 2022Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ZHAN-BIN LIU, BO SHEN
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Patent number: 11286496Abstract: Soybean seeds with increased protein, oil or protein plus oil and having a modified expression of a reticulon-like polypeptide, modified expression of a trehalose-6-phosphate synthase polypeptide, or modified expression of both sequences are provided. Methods for modifying expression of reticulon-like polypeptides and polynucleotides and trehalose-6-phosphate synthase polypeptides and polynucleotides include genome editing to modify the transcription regulatory region or sequence encoding the reticulon-like and trehalose-6-phosphate synthase polypeptides and transformation with recombinant DNA constructs to enhance or suppress expression.Type: GrantFiled: October 30, 2019Date of Patent: March 29, 2022Inventors: Zhan-Bin Liu, Bo Shen
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Publication number: 20220030788Abstract: The field is molecular biology, and more specifically, methods for editing the genome of a plant cell to identify causal alleles of a desired trait or to fine map a desired trait to small region of the genome for gene identification.Type: ApplicationFiled: September 13, 2019Publication date: February 3, 2022Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: SABRINA HUMBERT, MARK TIMOTHY JUNG, ZHAN-BIN LIU, ROBERT B MEELEY, BO SHEN, MARISSA SIMON, PETRA J WOLTERS
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Publication number: 20210403933Abstract: Soybean seeds with increased protein and having a modified expression or activity of at least one or two HECT E3 ligase polypeptides are provided. Methods for modifying expression or activity of HECT E3 ligase polypeptides and polynucleotides include genome editing to modify the transcription regulatory region or sequence encoding the HECT E3 ligase polypeptides and transformation with recombinant DNA constructs to enhance or suppress expression or activity of the HECT E3 ligase polypeptides. Plants containing the modifications produce seeds with altered composition such as one or more of increased protein, decreased soluble carbohydrate, increased oleic acid, decreased saturated fats such as palmitic and stearic acids, and decreased linoleic or linolenic acid.Type: ApplicationFiled: November 12, 2019Publication date: December 30, 2021Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: JOHN RUSSELL BOOTH, Jr., ZHAN-BIN LIU, CHENG LU, BO SHEN
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Publication number: 20210395760Abstract: Methods and compositions for increasing, improving or enhancing gene editing efficiency are provided. Configurations of Ochrobactrum and Agrobacterium based vector components such as CRISPR Cas endonucleases and guide RNAs are provided that improve efficiency of targeted genome modification.Type: ApplicationFiled: October 30, 2019Publication date: December 23, 2021Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: JYOTHI BOLAR, SHUJUN CHANG, HYEON-JE CHO, ZHAN-BIN LIU
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Publication number: 20210238614Abstract: Methods and compositions are provided for the improvement of homology-directed repair of a double strand break in a plant cell, via the use of a polynucleotide comprising sequences homologous to the target site. In some aspects, the double strand break is created by an RNA-guided Cas endonuclease. The homology-directed repair of the double-strand break may include incorporation of a heterologous polynucleotide, for example a gene encoding a trait of agronomic importance. The homology-directed repair of the double-strand break may occur as a result of template-directed repair using a polynucleotide repair template.Type: ApplicationFiled: May 7, 2019Publication date: August 5, 2021Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: AJITH ANAND, WILLIAM JAMES GORDON-KAMM, SANDEEP KUMAR, ZHAN-BIN LIU, SERGI SVITASHEV
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Publication number: 20210207153Abstract: Systems and methods for editing plant genomes include systems and methods for transforming plants generally, and Brassica plants particularly, with genome editing systems including CRISPR-Cas. Systems and methods for editing plant genomes include systems and methods for transforming Brassica microspores with genome editing systems and culturing the plant microspores that increase genome editing frequency.Type: ApplicationFiled: May 30, 2019Publication date: July 8, 2021Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: David George Charne, Wenpin Chen, James Brent Gillespie, Siva S. Ammirajo Jetty, Zhan-Bin Liu, Gina Marie Zastrow-Hayes
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Publication number: 20210207103Abstract: Provided are compositions comprising polynucleotides encoding modified diacylglycerol acyltransferase-1 (DGAT1) polypeptides having improved properties, such as increased enzymatic activity and/or increased stability. Plants, plant cells, seed, grain and comprising the polynucleotides are provided which have one or more of increased fatty acid or protein content. Methods of generating the polynucleotides in plant cells include transformation and genetic modification. Methods of employing the polynucleotides in plants, methods for increasing DGAT1 activity in a plant, and methods for increasing fatty acid content or protein content in a plant are provided.Type: ApplicationFiled: May 30, 2019Publication date: July 8, 2021Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: KAYLA S FLYCKT, KRISTIN HAUG COLLET, ZHAN-BIN LIU, KEITH R ROESLER, BO SHEN, LAURA L WAYNE
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Publication number: 20210062201Abstract: Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.Type: ApplicationFiled: July 10, 2020Publication date: March 4, 2021Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E. I. DU PONT DE NEMOURS AND COMPANYInventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, MICHAEL LASSNER, DEREK JANTZ, ZHONGSEN LI, ZHAN-BIN LIU, SERGEI SVITASHEV, JAMES JEFFERSON SMITH
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Publication number: 20200399648Abstract: Methods and compositions to modify the fatty acid profiles of seeds and the oils produced therefrom include the modification of four or more alleles of fatty acid desaturases in the genome of a plant. Compositions, polynucleotide constructs, transformed and modified host cells, and plants and seeds exhibiting altered fatty characteristics are provided.Type: ApplicationFiled: March 1, 2019Publication date: December 24, 2020Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: JOHN D EVERARD, ANTHONY J KINNEY, ZHAN-BIN LIU
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Patent number: 10870859Abstract: The disclosure relates to gene expression regulatory sequences from maize, specifically to the promoter of a maize U6 polymerase III gene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, mutated plants, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.Type: GrantFiled: November 5, 2019Date of Patent: December 22, 2020Inventors: Zhongsen Li, Zhan-Bin Liu
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Patent number: 10822610Abstract: Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.Type: GrantFiled: June 13, 2018Date of Patent: November 3, 2020Inventors: Andrew Mark Cigan, Saverio Carl Falco, Huirong Gao, Michael Lassner, Derek Jantz, Zhongsen Li, Zhan-Bin Liu, Sergei Svitashev, James Jefferson Smith
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Patent number: 10676754Abstract: The present disclosure includes the production of a mutant plant resistant to an herbicide of the phosphonomethylglycine family, e.g. glyphosate. Compositions and methods are provided for editing a nucleotide sequence of interest in a cell employing a guide polynucleotide/Cas endonuclease system, wherein the Cas endonuclease is guided by a guide polynucleotide to recognize and optionally introduce a double strand break at a specific target site into the genome of a cell. The nucleotide sequence of interest to be edited can be located within or outside the target site that is recognized by a Cas endonuclease. More specifically, compositions and methods are provided for editing an enolpyruvylshikimate-3-phosphate synthase (EPSPS) nucleotide sequence in a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide for an effective system for editing EPSPS nucleotide sequences of within the genome of a cell.Type: GrantFiled: July 1, 2015Date of Patent: June 9, 2020Assignees: E I DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Vesna Djukanovic, Spencer Charles Jones, Zhan-Bin Liu, Michael Lassner, Leszek Aleksander Lyznik
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Publication number: 20200157554Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed.Type: ApplicationFiled: January 30, 2020Publication date: May 21, 2020Applicants: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, ZHONGSEN LI, ZHAN-BIN LIU, L. ALEKSANDER LYZNIK, JINRUI SHI, SERGEI SVITASHEV, JOSHUA K. YOUNG
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Publication number: 20200063151Abstract: The disclosure relates to gene expression regulatory sequences, specifically to the promoter of a U6 polymerase III gene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, mutated plants, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.Type: ApplicationFiled: November 5, 2019Publication date: February 27, 2020Applicant: E. I. DU PONT DE NEMOURS AND COMPANYInventors: ZHONGSEN LI, ZHAN-BIN LIU
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Patent number: 10519457Abstract: The disclosure relates to gene expression regulatory sequences from soybean, specifically to the promoter of a soybean U6 polymerase III gene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, mutated plants, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.Type: GrantFiled: August 20, 2014Date of Patent: December 31, 2019Assignee: E I DU PONT DE NEMOURS AND COMPANYInventors: Zhongsen Li, Zhan-Bin Liu
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Publication number: 20190376074Abstract: The disclosure relates to gene expression regulatory sequences, specifically to the promoter of a U6 polymerase III gene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, mutated plants, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.Type: ApplicationFiled: August 27, 2019Publication date: December 12, 2019Applicant: E. I. DU PONT DE NEMOURS AND COMPANYInventors: ZHONGSEN LI, ZHAN-BIN LIU
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Publication number: 20190040372Abstract: Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.Type: ApplicationFiled: October 18, 2018Publication date: February 7, 2019Applicants: E. I. DUPONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Ericka Bermudez, Andrew Mark Cigan, James J English, Saverio Carl Falco, Huirong Gao, Lu Liu, Zhan-Bin Liu, Azalea S Ong, Sergei Svitashev, Joshua K Young