Abstract: This technology relates to the production of lactose and human milk oligosaccharides (HMOs) in cells.

Abstract: Disclosed is an expression vector comprising a polynucleotide encoding for a glutamine synthetase with reduced activity compared to a wild type glutamine synthetase. Also disclosed are host cells, methods for preparing stable cell line, methods of producing polypeptide of interest, and kits thereof.

Abstract: The present application discloses an autonomous machine and methods of controlling the autonomous machine. The present application also relates to a computing system for controlling movement of the autonomous machine. The autonomous machine comprises a movable component, a representation unit, a transformation unit communicatively coupled to the representation unit and a driving mechanism coupled to the movable component and the transformation unit for moving the movable component. In particular, poses of every part of the autonomous machine may be transformed back and forth by simply mathematical addition and subtraction.

Abstract: An array substrate, a manufacturing method thereof, and a display panel are provided. In the array substrate, a first metal layer includes a common electrode, the common electrode includes a first frame and a wire disposed in the first frame, the wire and the first frame are insulated from each other and spaced apart, a second metal layer and the first metal layer are disposed in different layers, the second metal layer includes a share electrode, and the share electrode is disposed overlapping the wire.

Abstract: A thin film transistor and an array substrate, including a light-shielding layer and an active layer. The light-shielding layer includes a first light-shielding pattern, a second light-shielding pattern and a third light-shielding pattern. The active layer includes a channel area, a first conductive area and a second conductive area located on both sides of the channel area. An orthogonal projection of the first light-shielding pattern on the substrate at least covers an orthogonal projection of the channel area on the substrate. An orthographic projection of the second light-shielding pattern on the substrate overlaps at least a part of an orthographic projection of the first conductive area on the substrate. An orthographic projection of the third light-shielding pattern on the substrate overlaps at least a part of an orthographic projection of the second conductive area on the substrate.

Abstract: The present application provides an organic light emitting diode (OLED) display panel and a method of manufacturing the same, and a display terminal. The OLED display panel includes an array substrate, a protective layer and a cathode. The array substrate includes a first auxiliary electrode, a second auxiliary electrode, and a third auxiliary electrode. The third auxiliary electrode is connected to the first auxiliary electrode and the second auxiliary electrode. The protective layer is provided with an undercut opening exposing a portion of the third auxiliary electrode. The cathode extends into the undercut opening and is connected to the third auxiliary electrode.

Abstract: Disclosed is an expression vector comprising a polynucleotide encoding for a glutamine synthetase with reduced activity compared to a wild type glutamine synthetase. Also disclosed are host cells, methods for preparing stable cell line, methods of producing polypeptide of interest, and kits thereof.

Abstract: Discussed are a flexible thin film transistor (TFT) substrate, a flexible display panel and a flexible display device. The flexible TFT substrate of the present application can improve bending resistance of the flexible TFT substrate by arranging a concave structure in the bonding region and placing an organic material in the concave structure. When the bonding region is bent, a width of the bonding region can be reduced. Accordingly, a bezel width of the flexible display panel including the flexible TFT substrate is reduced, and a narrow bezel design is realized.

Abstract: An array substrate, a manufacturing method thereof, and a display panel are provided. In the array substrate, a first metal layer includes a common electrode, the common electrode includes a first frame and a wire disposed in the first frame, the wire and the first frame are insulated from each other and spaced apart, a second metal layer and the first metal layer are disposed in different layers, the second metal layer includes a share electrode, and the share electrode is disposed overlapping the wire.

Abstract: An embodiment of the present application describes a base plate and a display panel, where a thin film transistor has a gate electrode, an active layer, and a source-drain metal layer. The active layer is arranged in a different layer from the gate electrode, and the active layer is disposed to overlap the gate electrode. The barrier layer at least covers the active layer and the gate electrode, and the barrier layer is used to block water and oxygen. The flat layer covers the barrier layer; and the source-drain metal layer is arranged on the flat layer. The source-drain metal layer has a source electrode and a drain electrode, and the source electrode and the drain electrode are respectively connected to the active layer.

Abstract: The present invention discloses a prediction method for a maximum velocity profile in a wave boundary layer based on velocity defect functions. The method overcomes the theoretical defects of the existing velocity defect functions. That is, the velocity profile in a turbulent wave boundary layer cannot be realized; and in addition, without the assumption of linear wave conditions, the method is suitable for nonlinear waves and at the same time, for a small A/k, range, and can be extended to the analysis and prediction for the maximum velocity profile under the condition that the spatial distribution of roughness elements of gravel seabed, etc. obviously affects the flow structure of the boundary layer. The present invention can be directly applied to the analysis and prediction for physical quantities/processes, such as characteristics of the wave boundary layer, stress of underwater structures, and starting and transport of submarine sediments.

Abstract: The present application discloses an autonomous machine and methods of controlling the autonomous machine. The present application also relates to a computing system for controlling movement of the autonomous machine. The autonomous machine comprises a movable component, a representation unit, a transformation unit communicatively coupled to the representation unit and a driving mechanism coupled to the movable component and the transformation unit for moving the movable component. In particular, poses of every part of the autonomous machine may be transformed back and forth by simply mathematical addition and subtraction.

Abstract: A virtual co-phase power supply system topology suitable for an electrical sectioning device at a sectioning and paralleling post (SP) includes a step-down transformer TR1. A primary winding of the step-down transformer TR1 is electrically connected to a traction feeding section ?2 in a train from a traction feeding section ?1 to the traction feeding section ?2. Each secondary winding is electrically connected to one rectifier separately. DC buses output from the rectifiers are connected in parallel. The other end of the DC bus is electrically connected to a plurality of parallel inverter units. An LC filter is provided on a DC bus between a rectifier unit and the inverter unit, and the LC filter is connected in parallel to an energy storage unit. After filtering through the LC filter, an output end of the inverter unit is electrically connected to a primary winding of a step-up transformer TR2.

Abstract: A method of sequential mapping and localization (SMAL) is disclosed for navigating a mobile object (i.e. SMAL method). The SMAL method comprises a step of generating an initial map of an unknown environment in a mapping process; a step of determining a location of the mobile object in the initial map in a localization process; and a step of guiding the mobile object in the unknown environment, e.g. by creating a control or an instruction to the mobile object. A system using the SMAL method and a computer program product for implementing the SMAL method are also disclosed accordingly.

Abstract: A reinforcement learning (RL) based adaptive state observation model usable for implementing a brain machine interface (BMI) is proposed for decoding a brain signal to determine a movement action and controlling a machine to perform the movement action. In the model, the brain signal is processed by a neural network (NN) for applying a nonlinear mapping defined by NN weights to the brain signal to thereby yield a transformed brain signal. The NN learns the nonlinear mapping by RL, allowing the weights to be adaptively and continuously updated to follow nonlinearity and non-stationarity of the brain signal. The transformed brain signal is processed by a Kalman filter (KF) to yield a control signal for controlling the machine to perform the movement action, thereby utilizing the KF to provide smooth generation of the control signal while blocking adverse influence of nonlinearity and non-stationarity of the brain signal to the KF.

Abstract: A modified modulated wave acquisition method includes: obtaining a modulated wave uaba; calculating a difference between the given value iNq* and the actual value iNq of the q-axis component of a grid side current, inputting the result to a proportional integral (PI) controller, and multiplying an output of the PI controller by cos ?t to obtain a modulated wave offset ?uaba; and calculating a difference between the modulated wave uaba and the modulated wave offset ?uaba to obtain a modified modulation wave uaba?, where ?t is a grid voltage phase in a sinusoidal case. The MPC method for a single-phase cascaded H-bridge rectifier includes: obtaining the modified modulated wave uaba?, where the component iNq* is 0; and replacing the modulated wave uaba with the modified modulated wave uaba? to perform MPC for the single-phase cascaded H-bridge rectifier.

Abstract: A virtual co-phase power supply system topology suitable for an electrical sectioning device at a sectioning and paralleling post (SP) includes a step-down transformer TR1. A primary winding of the step-down transformer TR1 is electrically connected to a traction feeding section ?2 in a train from a traction feeding section ?1 to the traction feeding section ?2. Each secondary winding is electrically connected to one rectifier separately. DC buses output from the rectifiers are connected in parallel. The other end of the DC bus is electrically connected to a plurality of parallel inverter units. An LC filter is provided on a DC bus between a rectifier unit and the inverter unit, and the LC filter is connected in parallel to an energy storage unit. After filtering through the LC filter, an output end of the inverter unit is electrically connected to a primary winding of a step-up transformer TR2.

Abstract: A modified modulated wave acquisition method includes: obtaining a modulated wave uaba; calculating a difference between the given value iNq* and the actual value iNq of the q-axis component of a grid side current, inputting the result to a proportional integral (PI) controller, and multiplying an output of the PI controller by cos ?t to obtain a modulated wave offset ?uaba; and calculating a difference between the modulated wave uaba and the modulated wave offset ?uaba to obtain a modified modulation wave uaba?, where ?t is a grid voltage phase in a sinusoidal case. The MPC method for a single-phase cascaded H-bridge rectifier includes: obtaining the modified modulated wave uaba?, where the component iNq* is 0; and replacing the modulated wave uaba with the modified modulated wave uaba? to perform MPC for the single-phase cascaded H-bridge rectifier.

Abstract: Disclosed is an expression vector comprising a polynucleotide encoding for a glutamine synthetase with reduced activity compared to a wild type glutamine synthetase. Also disclosed are host cells, methods for preparing stable cell line, methods of producing polypeptide of interest, and kits thereof.

Abstract: There is provided a method of signal light detection, and a corresponding signal light detection device. The method includes obtaining a dark frame of an environment, the dark frame comprising a plurality of light blobs captured corresponding to lights captured in the environment, obtaining a bright frame of the environment, the bright frame comprising a plurality of light blobs corresponding to lights captured in the environment, identifying a plurality of candidate light blobs from the plurality of light blobs in the dark frame, identifying a plurality of candidate regions in the bright frame based on the plurality of candidate light blobs identified from the dark frame, classifying each of the plurality of candidate light blobs based on the corresponding candidate region from the bright frame, and evaluating whether one or more of the plurality of classified candidate light blobs is a signal light from a particular type of signaling device.