Abstract: A virtual co-phase power supply system topology suitable for an electrical sectioning device at a sectioning and paralleling post (SP) includes a step-down transformer TR1. A primary winding of the step-down transformer TR1 is electrically connected to a traction feeding section ?2 in a train from a traction feeding section ?1 to the traction feeding section ?2. Each secondary winding is electrically connected to one rectifier separately. DC buses output from the rectifiers are connected in parallel. The other end of the DC bus is electrically connected to a plurality of parallel inverter units. An LC filter is provided on a DC bus between a rectifier unit and the inverter unit, and the LC filter is connected in parallel to an energy storage unit. After filtering through the LC filter, an output end of the inverter unit is electrically connected to a primary winding of a step-up transformer TR2.

Abstract: A modified modulated wave acquisition method includes: obtaining a modulated wave uaba; calculating a difference between the given value iNq* and the actual value iNq of the q-axis component of a grid side current, inputting the result to a proportional integral (PI) controller, and multiplying an output of the PI controller by cos ?t to obtain a modulated wave offset ?uaba; and calculating a difference between the modulated wave uaba and the modulated wave offset ?uaba to obtain a modified modulation wave uaba?, where ?t is a grid voltage phase in a sinusoidal case. The MPC method for a single-phase cascaded H-bridge rectifier includes: obtaining the modified modulated wave uaba?, where the component iNq* is 0; and replacing the modulated wave uaba with the modified modulated wave uaba? to perform MPC for the single-phase cascaded H-bridge rectifier.

Abstract: Disclosed is an expression vector comprising a polynucleotide encoding for a glutamine synthetase with reduced activity compared to a wild type glutamine synthetase. Also disclosed are host cells, methods for preparing stable cell line, methods of producing polypeptide of interest, and kits thereof.

Abstract: There is provided a method of signal light detection, and a corresponding signal light detection device. The method includes obtaining a dark frame of an environment, the dark frame comprising a plurality of light blobs captured corresponding to lights captured in the environment, obtaining a bright frame of the environment, the bright frame comprising a plurality of light blobs corresponding to lights captured in the environment, identifying a plurality of candidate light blobs from the plurality of light blobs in the dark frame, identifying a plurality of candidate regions in the bright frame based on the plurality of candidate light blobs identified from the dark frame, classifying each of the plurality of candidate light blobs based on the corresponding candidate region from the bright frame, and evaluating whether one or more of the plurality of classified candidate light blobs is a signal light from a particular type of signaling device.

Abstract: Antibodies of interest comprising a heavy chain signal peptide and/or a light chain signal peptide or combination thereof and compositions are described. The method of determining the signal peptide including creating data set of antibodies signal peptides, clustering, selecting and creating recombinant antibodies for enhanced expression and secretion are described.

Abstract: We describe a method of reducing batch-to-batch variation or increasing homogeneity between batches in the production of a recombinantly expressed polypeptide. The method comprises expressing the polypeptide in a Chinese hamster ovary (CHO) cell comprising reduced UDP-galactose transporter activity compared to a wild-type CHO cell. In some embodiments, the CHO cell further comprises reduced GDP-fucose transporter activity.

Abstract: We provide a Chinese Hamster Ovary (CHO) cell which is capable of higher protein sialylation compared to a wild type Chinese Hamster Ovary cell, such as in the presence of functional GnT 1, in which the CHO cell is obtainable by selection with Ricinus communis agglutinin I (RCA-I).

Abstract: We describe a method of expressing a recombinant protein comprising mannose-terminated N-glycans from a host cell, the method comprising: (a) introducing a nucleic acid encoding a recombinant protein into a Chinese Hamster Ovary (CHO) cell comprising a mutation in the GnT 1 gene (GenBank Accession Number AF343963) leading to loss of GnT 1 function; and (c) expressing the recombinant protein from the host cell, in which the expressed recombinant protein comprises a mannose-terminated glycan structure, and in which the method does not include a step of introducing functional GnT-I into the host cell. The method may be used for producing recombinant glucocerebrosidase with a mannose-terminated glycan structure, suitable for treatment or prevention of Gaucher's Disease.

Abstract: We describe a method of expressing a recombinant protein comprising mannose-terminated N-glycans from a host cell, the method comprising: (a) introducing a nucleic acid encoding a recombinant protein into a Chinese Hamster Ovary (CHO) cell comprising a mutation in the GnT 1 gene (GenBank Accession Number AF343963) leading to loss of GnT 1 function; and (c) expressing the recombinant protein from the host cell, in which the expressed recombinant protein comprises a mannose-terminated glycan structure, and in which the method does not include a step of introducing functional GnT-1 into the host cell. The method may be used for producing recombinant glucocerebrosidase with a mannose-terminated glycan structure, suitable for treatment or prevention of Gaucher's Disease.

Abstract: Antibodies of interest comprising a heavy chain signal peptide and/or a light chain signal peptide or combination thereof and compositions are described. The method of determining the signal peptide including creating data set of antibodies signal peptides, clustering, selecting and creating recombinant antibodies for enhanced expression and secretion are described.

Abstract: We provide a Chinese Hamster Ovary (CHO) cell which is capable of higher protein sialylation compared to a wild type Chinese Hamster Ovary cell, such as in the presence of functional GnT 1, in which the CHO cell is obtainable by selection with Ricinus communis agglutinin I (RCA-I).

Abstract: We describe a method of expressing a recombinant protein comprising mannose-terminated N-glycans from a host cell, the method comprising: (a) introducing a nucleic acid encoding a recombinant protein into a Chinese Hamster Ovary (CHO) cell comprising a mutation in the GnT 1 gene (GenBank Accession Number AF343963) leading to loss of GnT 1 function; and (c) expressing the recombinant protein from the host cell, in which the expressed recombinant protein comprises a mannose-terminated glycan structure, and in which the method does not include a step of introducing functional GnT-I into the host cell. The method may be used for producing recombinant glucocerebrosidase with a mannose-terminated glycan structure, suitable for treatment or prevention of Gaucher's Disease.

Abstract: The present invention provides modified cells for producing proteins with modified glycosylation patterns. Proteins produced in such cells, and the use of such proteins in medicine, and particularly in the treatment of cancer, is also provided.

Abstract: We provide a Chinese Hamster Ovary (CHO) cell which is capable of higher protein sialylation compared to a wild type Chinese Hamster Ovary cell, such as in the presence of functional GnT 1, in which the CHO cell is obtainable by selection with Ricinus communis agglutinin I (RCA-I).

Abstract: We describe a method of expressing a recombinant protein comprising mannose-terminated N-glycans from a host cell, the method comprising: (a) introducing a nucleic acid encoding a recombinant protein into a Chinese Hamster Ovary (CHO) cell comprising a mutation in the GnT 1 gene (GenBank Accession Number AF343963) leading to loss of GnT 1 function; and (c) expressing the recombinant protein from the host cell, in which the expressed recombinant protein comprises a mannose-terminated glycan structure, and in which the method does not include a step of introducing functional GnT-1 into the host cell. The method may be used for producing recombinant glucocerebrosidase with a mannose-terminated glycan structure, suitable for treatment or prevention of Gaucher's Disease.

Abstract: We provide a Chinese Hamster Ovary (CHO) cell which is capable of higher protein sialylation compared to a wild type Chinese Hamster Ovary cell, such as in the presence of functional GnT 1, in which the CHO cell is obtainable by selection with Ricinus communis agglutinin I (RCA-I).