Abstract: A pneumatic proportional valve includes a torque motor and a pneumatic rotary valve. The torque motor includes permanent magnets, magnetically permeable yokes, an upper armature, a lower armature, an upper skeleton, a skeleton column, a lower skeleton, an exciting coil, a coil skeleton, a spring support, a spring plate and load springs. The torque motor has a single coil structure with an axis of the exciting coil coinciding with a rotation axis of the armatures, and the coil is arranged between the upper armature and the lower armature. The torque motor has the upper armature and the lower armature, and the upper armature and the lower armature have a total of four armature arms, which can form eight air gaps with an upper one and a lower one working in pair, form four pairs of magnetic circuits in parallel with each other under the excitation of a control coil.

Abstract: A nano scale robotic system for single cell DNA sequencing of a strand of DNA positioned on a slide utilizes an atomic force microscope (AFM) having an end effector in the form of a cantilever with a tip. The AFM causes its cantilever tip to scan over the base pairs of the DNA strand. A pair of spaced-apart electrodes at the tip makes contact with opposite sides of the DNA strand and the current between bases of the DNA strand is measured by a current measurement system connected to the electrodes. An artificial intelligence-based data analytic system determines the DNA sequence based on the current from the current measuring system. The AFM tip is guided over the DNA strand by comparing compressed desired intensity local scan images and compressed actual intensity local scan images and using the difference to control the location of the tip.

Abstract: A pneumatic proportional valve includes a torque motor and a pneumatic rotary valve. The torque motor includes permanent magnets, magnetically permeable yokes, an upper armature, a lower armature, an upper skeleton, a skeleton column, a lower skeleton, an exciting coil, a coil skeleton, a spring support, a spring plate and load springs. The torque motor has a single coil structure with an axis of the exciting coil coinciding with a rotation axis of the armatures, and the coil is arranged between the upper armature and the lower armature. The torque motor has the upper armature and the lower armature, and the upper armature and the lower armature have a total of four armature arms, which can form eight air gaps with an upper one and a lower one working in pair, form four pairs of magnetic circuits in parallel with each other under the excitation of a control coil.

Abstract: A large-flux in-situ filtration device for marine microorganism based on a multi-channel circulation distributor is provided, comprising an energy and control module, a circulation distribution module, a filtration module, a pump module, a filtration volume measurement module, a pipeline connection, and a main body support frame. The circulation distribution module may include a multi-channel circulation distributor, a deep-sea servo motor, etc. The filtration module may include a plurality of filter film holders. The pump module may include a stainless steel gear pump, a deep-sea drive motor, etc. The filtration volume measurement module may include a water meter, a camera, and an LED light.

Abstract: Provided herein are methods of producing oils with reduced saturated fatty acids. The methods include culturing oil-producing microorganisms in a fermentation medium in the presence of one or more antifoaming agents under a controlled carbon consumption rate, wherein the culturing produces oils comprising fatty acids and wherein less than 35% of the fatty acids in the oil are saturated fatty acids.

Abstract: Provided herein are methods of culturing a microorganism. The method includes providing a container comprising one or more microorganisms and medium, wherein the microorganisms and medium form a start volume. The microorganisms and medium are cultured until the culture reaches a threshold indicator, wherein culturing comprises feeding one or more carbon sources to the culture and wherein the culture is at a threshold volume when the threshold indicator is reached. The method also includes harvesting a portion of the threshold volume to leave a residual volume that is 40% or less of the start volume and adding fresh medium to the container in an amount to return the volume of the culture to the start volume.

Abstract: The present disclosure involves a calculation method and device for intelligent cutting squid white slices, the calculation method being implemented on the device for intelligent cutting squid white slices. The calculation method may be implemented on a calculation device, the calculation device may have at least one processor and at least one storage medium including an instruction set used for intelligent cutting the squid white slices, the calculation method including: reading a laser point cloud data of a three-dimensional (3D) topography of the squid white slices; optimizing the laser point cloud data; extracting an effective area of the squid white slices; determining a cutting zero point; determining a cutting process area; and determining optimization of a cutting point position and a cutting angle. The one or more embodiments provided by the present disclosure may satisfy the needs of large-scale continuous production in factories, reduce labor costs, and improve production efficiency.

Abstract: Provided herein are methods of culturing a microorganism. The method includes providing a container comprising one or more microorganisms and medium, wherein the microorganisms and medium form a start volume. The microorganisms and medium are cultured until the culture reaches a threshold indicator, wherein culturing comprises feeding one or more carbon sources to the culture and wherein the culture is at a threshold volume when the threshold indicator is reached. The method also includes harvesting a portion of the threshold volume to leave a residual volume that is 40% or less of the start volume and adding fresh medium to the container in an amount to return the volume of the culture to the start volume.

Abstract: The present disclosure involves a calculation method and device for intelligent cutting squid white slices, the calculation method being implemented on the device for intelligent cutting squid white slices. The calculation method may be implemented on a calculation device, the calculation device may have at least one processor and at least one storage medium including an instruction set used for intelligent cutting the squid white slices, the calculation method including: reading a laser point cloud data of a three-dimensional (3D) topography of the squid white slices; optimizing the laser point cloud data; extracting an effective area of the squid white slices; determining a cutting zero point; determining a cutting process area; and determining optimization of a cutting point position and a cutting angle. The one or more embodiments provided by the present disclosure may satisfy the needs of large-scale continuous production in factories, reduce labor costs, and improve production efficiency.

Abstract: A nano scale robotic system for single cell DNA sequencing of a strand of DNA positioned on a slide utilizes an atomic force microscope (AFM) having an end effector in the form of a cantilever with a tip. The AFM causes its cantilever tip to scan over the base pairs of the DNA strand. A pair of spaced-apart electrodes at the tip makes contact with opposite sides of the DNA strand and the current between bases of the DNA strand is measured by a current measurement system connected to the electrodes. An artificial intelligence-based data analytic system determines the DNA sequence based on the current from the current measuring system. The AFM tip is guided over the DNA strand by comparing compressed desired intensity local scan images and compressed actual intensity local scan images and using the difference to control the location of the tip.

Abstract: A large-flux in-situ filtration device for marine microorganism based on a multi-channel circulation distributor is provided, comprising an energy and control module, a circulation distribution module, a filtration module, a pump module, a filtration volume measurement module, a pipeline connection, and a main body support frame. The circulation distribution module may include a multi-channel circulation distributor, a deep-sea servo motor, etc. The filtration module may include a plurality of filter film holders. The pump module may include a stainless steel gear pump, a deep-sea drive motor, etc. The filtration volume measurement module may include a water meter, a camera, and an LED light.

Abstract: The present disclosure involves an intelligent method and device for cutting squid white slices, the intelligent method being implemented on the intelligent device for cutting squid white slices. The method may be implemented on a calculating device, the calculating device may have at least one processor and at least one storage medium including an instruction set used for cutting the squid white slices, the method including: reading a laser point cloud data of a three-dimensional (3D) topography of the squid white slices; optimizing the laser point cloud data; extracting an effective area of the squid white slices; determining a cutting zero point; determining a cutting process area; and determining optimization of a cutting point position and a cutting angle. The one or more embodiments provided by the present disclosure may satisfy the needs of large-scale continuous production in factories, reduce labor costs, and improve production efficiency.

Abstract: An electromagnetic heating smoking paraphernalia capable of evenly heating tobacco particles includes a tobacco particle heating tank, wherein the tobacco particle heating tank includes a metal inductive cylinder and a tight coil, a space between the metal inductive cylinder and the tight coil is a tobacco particle holding cavity; an end of the tobacco particle holding cavity is provided with an upper end wall, the upper end wall is provided with an upper end wall vent hole, the other end of the tobacco particle holding cavity is provided with a lower end wall, the lower end wall is provided with a lower end wall vent hole; the tight coil means that adjacent turns of the coil are tightly placed close to each other in a longitudinal axial direction without a gap; an inner wall of the metal inductive cylinder is provided with an inner wall vent hole.

Abstract: Provided herein are methods of producing oils with reduced saturated fatty acids. The methods include culturing oil-producing microorganisms in a fermentation medium in the presence of one or more antifoaming agents under a controlled carbon consumption rate, wherein the culturing produces oils comprising fatty acids and wherein less than 35% of the fatty acids in the oil are saturated fatty acids.

Abstract: The present disclosure involves an intelligent method and device for cutting squid white slices, the intelligent method being implemented on the intelligent device for cutting squid white slices. The method may be implemented on a calculating device, the calculating device may have at least one processor and at least one storage medium including an instruction set used for cutting the squid white slices, the method including: reading a laser point cloud data of a three-dimensional (3D) topography of the squid white slices; optimizing the laser point cloud data; extracting an effective area of the squid white slices; determining a cutting zero point; determining a cutting process area; and determining optimization of a cutting point position and a cutting angle. The one or more embodiments provided by the present disclosure may satisfy the needs of large-scale continuous production in factories, reduce labor costs, and improve production efficiency.

Abstract: Provided is a method of culturing one or more microorganisms. The method includes culturing one or more microorganisms in a medium comprising crude glycerol at a first concentration level, feeding to the media an additional amount of crude glycerol, once the first concentration of glycerol is reduced to a first threshold level, at a concentration sufficient to achieve the first concentration level, monitoring the crude glycerol concentration until the first concentration level of the crude glycerol is reduced to the first threshold level. The steps may be repeated until a desired microorganism cell density is achieved.

Abstract: A high-precision control system and method for shipborne cryogenic flash freezing of an aquatic product using liquid nitrogen is described. The system may include a main control system, a display unit, a liquid nitrogen supply system, a valve control unit, an acquisition unit, and a power unit. A flash freezing process is divided into four stages: a precooling stage, a flash freezing stage, a deep freezing stage, and a thermal insulation stage. Different cooling rates and flash freezing times are used for different stages, where a cooling rate is used in the flash freezing stage is the highest, a cooling rate used in the deep freezing stage is next, a cooling rate used in the precooling stage is the lowest, and an ambient temperature in a device is kept stable in the thermal insulation stage.

Abstract: Provided herein are methods of culturing a microorganism. The methods include providing a container comprising one or more microorganisms in a medium, which has a first carbon to nitrogen ratio; culturing the microorganisms until the culture reaches a threshold indicator; harvesting a portion of the culture while maintaining the majority of the culture in the container; and adding fresh medium comprising a second carbon to nitrogen ratio to the container with the majority of the culture comprising the microorganisms.

Abstract: Provided herein are methods for producing one or more polyunsaturated fatty acids. The methods include the steps of providing a microorganism capable of producing polyunsaturated fatty acids, providing a medium comprising a high concentration of one or more carbon sources, low pH, or both, and culturing the microorganism in the medium under sufficient conditions to produce the one or more polyunsaturated fatty acids. Also provided are methods of culturing one or more microorganisms. The methods include culturing the microorganisms in a medium comprising a first amount of one or more carbon sources at a first concentration level, monitoring a carbon source concentration until the carbon source concentration is reduced below the first concentration level, and adding to the medium a second amount of one or more carbon sources to increase the carbon source concentration to a second concentration level.