Abstract: The present invention provides plants, plant cells and methods of making transgenic plants or plant cells, wherein the plants or cells are resistant to bacterial blight. The invention provides isolated nucleic acid sequences comprising SEQ ID NO: 1 or fragments thereof which provide a plant with resistance to bacterial blight disease when transfected into the plant.

Abstract: Provided are compounds capable of covalently binding to a protein tyrosine phosphatase (PTP). The compounds comprise Formula A: Also provided are compositions comprising one of the above compounds covalently bound to a member of the PTP superfamily, methods of labeling a PTP using the compounds, methods of isolating a PTP from a mixture of proteins using the compounds, methods of evaluating whether a substance is an inhibitor of a PTP using the compounds, methods of evaluating the specificity of an inhibitor of a PTP using the compounds, methods of identifying a PTP involved in a disease in a mammal using the compounds, and methods of diagnosing a disease in a mammal using the compounds.

Abstract: Methods for discovery of enzyme ligands and inhibitors are disclosed. The methods comprise the creation and testing of combinatorial libraries comprising an active site-targeted component, a linker component and a peripheral site-targeted component. The methods also comprise a novel assay for determining whether a compound is a ligand of an enzyme. The assay evaluates whether the compound can inhibit the binding of a known ligand of the active site of the enzyme to a mutant of the enzyme that can bind the enzyme substrate but cannot catalyze an enzymatic reaction with the substrate. Various ligands and inhibitors of protein tyrosine phosphatase 1B (PTP1B) are also disclosed. These ligands and inhibitors were discovered using the above methods. One particular inhibitor discovered using the invention methods has the highest specificity and affinity of any PTP1B inhibitor discovered to date.

Abstract: The present invention provides protein tyrosine phosphatases (PTP) in which the invariant aspartate residue and the invariant glutamine residue are each replaced with a replacement amino acid residue, wherein the replacement residues together cause a reduction in catalytic rate (kcat) of the enzyme and an increase in substrate-binding affinity (Kd) of the enzyme. The present invention further provides methods for identifying a substrate of a PTP. Also provided are kits for identifying a substrate of a PTP. Additionally, the present invention provides methods for identifying an agent that alters interaction between a PTP and a substrate of the PTP. The invention also provides methods for reducing the activity of a substrate of a PTP.

Abstract: Computer apparatus 10 includes mother board 10M, bracket 12 mounted on the mother board, a circuit board assembly 10A installed in the bracket, and a pair of assembly latches 13L and 13R. The mother board has connector slot 12E for electrically engaging corresponding contacts 10E on the circuit board assembly. The bracket has an elongated base member 12M positioned proximate the connector slot, and a pair of spaced retaining posts 12L and 12R extending generally away from the mother board, one at each end of the base member. The bracket receives the circuit board assembly into the space between the retaining posts. The circuit board assembly has heat conductive thermal plate 14 between circuit board 10C and heat sink 14H. The thermal plate has two end edges 14L and 14R, one proximate each retaining post, which are engaged by the retaining posts during and after installation. Each retaining post has a latch port 12P extending therethrough.

Abstract: Computer apparatus 10 receives add-on electronic module 12 for installation into the computer apparatus. The add-on module has first end 12A and second end 12B with electrical contacts 12C therebetween for interfacing with the computer apparatus. Elongated electrical connector 14 mounted on the mother board, has first end 14A and second end 14B corresponding to the first end and second end of the add-on module. The electrical connector defines a module site within the computer apparatus for receiving the add-on module. The electrical connector has electrical contacts 14C between the ends thereof for electrically contacting corresponding electrical contacts 12C on the add-on module. First pivot base 16A secured to the mother board proximate the first end of the electrical connector defines first pivot axis 17A. Second pivot base 16B proximate the second end of the electrical connector defines second pivot axis 17B.

Abstract: Computer apparatus 10 has enhanced convective flow 10F of circulating cooling air provided by improved fan 12. Fan support 12S is secured to the computer apparatus proximate fan port 10P formed in the housing. Fan blade 22B is rotatably mounted within fan support 22S. The propelling action of the fan blade defines a transition path for the cooling air through the fan. Stationary screen 24S is positioned adjacent to the blade traversing the transition path. Transition chamber 26 formed between the screen and the blade also traverses the transition path. The width of the transition chamber defines a transition gap dimension from the screen and to the blade that is optimally selected to enhance the cooling air flow. The transition chamber has a screen region 26S adjacent the screen and a blade region 26B adjacent the blade, and a middle region 26M between the screen region and the blade region. Cooling air flow through the screen and the screen region is generally linear.