Abstract: Provided herein are regulatory elements, such as promoters and terminators, derived or obtained from various Glycine species. Such regulatory elements are useful for expression cassettes for plants, such as soybean. Such expression cassettes generally contain a promoter and terminator sequence to control expression of each coding sequence. For certain traits, such as insect resistance and herbicide tolerance, it may be desirable to use promoters and terminators with medium to high constitutive expression.

Abstract: Provided herein are regulatory elements, such as transcriptional enhancers, introns, and terminators derived or obtained from various sources including Zea mays and plant viruses. Such regulatory elements are useful for expression cassettes for plants, including monocots (e.g., maize) and dicots (e.g., soy). Enhancers which increase gene expression of tissue specific promoters while maintaining tissue specific expression are provided.

Abstract: Provided herein are regulatory elements, such as promoters and terminators, derived or obtained from various Glycine species. Such regulatory elements are useful for expression cassettes for plants, such as soybean. Such expression cassettes generally contain a promoter and terminator sequence to control expression of each coding sequence. Provided are promoters and terminators which are useful for expressing insect resistance and herbicide tolerance genes.

Abstract: The present invention relates to methods and compositions for modifying a target site in the genome of a plant cell. Such modifications include integration of a transgene and mutations. The present invention also relates to methods and compositions for identifying and enriching for cells which comprise a modified target site.

Abstract: Provided herein are promoters and related compositions and methods of use. Such promoters are useful for expression cassettes for plants, such as soybean. Such expression cassettes are useful, e.g., to drive expression of trait genes in plants, such as soybean. In some aspects, the disclosure provides an expression cassette comprising a promoter, a 5? UTR, and an intron.

Abstract: The present invention relates to methods and compositions for modifying a target site in the genome of a plant cell. Such modifications include integration of a transgene and mutations. The present invention also relates to methods and compositions for identifying and enriching for cells which comprise a modified target site.

Abstract: The present invention relates to methods and compositions for modifying a target site in the genome of a plant cell. Such modifications include integration of a transgene and mutations. The present invention also relates to methods and compositions for identifying and enriching for cells which comprise a modified target site.

Abstract: The invention provides three novel disarmed strains of Agrobacteriumtumefaciens bacteria useful for the transformation of plants. The invention provides three engineered A. tumefaciens Chry5 strains or bacterial cells thereof which comprise the Chry5 strain chromosomal background and a disarmed pTiChry5 vector, and methods of using said bacterial strains or cells for transformation of fungal or plant cells, in particular dicot or monocot plant cells, including soybean, maize, wheat, and sugarcane cells. The invention further relates to the transgenic plants created by these methods.

Abstract: The present invention relates to methods and compositions for targeted insertion of polynucleotide molecules into ideal target sites in the genome of a maize plant. The present invention relates to maize recombinant molecules comprising heterologous sequences and also to methods of integrating a DNA of interest into a target maize genomic locus in a maize genome. The present invention also relates to regenerated maize plants or plant parts comprising the recombinant molecules and/or a DNA of interest.

Abstract: Provided herein are regulatory elements, such as promoters and terminators, derived or obtained from various Glycine species. Such regulatory elements are useful for expression cassettes for plants, such as soybean. Such expression cassettes generally contain a promoter and terminator sequence to control expression of each coding sequence. For certain traits, such as insect resistance and herbicide tolerance, it may be desirable to use promoters and terminators with medium to high constitutive expression.

Abstract: The invention provides three novel disarmed strains of Agrobacterium tumefaciens bacteria useful for the transformation of plants. The invention provides three engineered A. tumefaciens Chry5 strains or bacterial cells thereof which comprise the Chry5 strain chromosomal background and a disarmed pTiChry5 vector, and methods of using said bacterial strains or cells for transformation of fungal or plant cells, in particular dicot or monocot plant cells, including soybean, maize, wheat, and sugarcane cells. The invention further relates to the transgenic plants created by these methods.

Abstract: The present invention relates to methods and compositions for targeted insertion of polynucleotide molecules into ideal target sites in the genome of a maize plant. The present invention relates to maize recombinant molecules comprising heterologous sequences and also to methods of integrating a DNA of interest into a target maize genomic locus in a maize genome. The present invention also relates to regenerated maize plants or plant parts comprising the recombinant molecules and/or a DNA of interest.

Abstract: Systems and methods for producing a plurality of unique edits in a plant's T1 seed. In one example, a method comprises transforming at least one expression cassette into a plant cell or a plant tissue. The at least one expression cassette may comprise a nucleic acid that encodes a DNA modification enzyme; optionally, a nucleic acid that encodes at least one guide RNA (gRNA); and a floral mosaic (FMOS) regulatory sequence, wherein the FMOS regulatory sequence (i) mediates expression of the DNA modification enzyme in at least one of a floral primordia cell and a floral reproductive organ, and (ii) mediates a plurality of edits in the at least one of the floral primordia and the floral reproductive organ. The method may also include regenerating the plant cell or plant tissue into a T0 plant having a plurality of T1 seed, wherein the T1 seed contain a plurality of unique edits.

Abstract: The present invention relates to methods and compositions for modifying a target site in the genome of a plant cell. Such modifications include integration of a transgene and mutations. The present invention also relates to methods and compositions for identifying and enriching for cells which comprise a modified target site.

Abstract: The present invention relates to methods and compositions for targeted insertion of polynucleotide molecules into ideal target sites in the genome of a maize plant. The present invention relates to maize recombinant molecules comprising heterologous sequences and also to methods of integrating a DNA of interest into a target maize genomic locus in a maize genome. The present invention also relates to regenerated maize plants or plant parts comprising the recombinant molecules and/or a DNA of interest.

Abstract: The present invention relates to methods and compositions for chimeric RNA comprising a guide RNA and bait RNA for modifying a target site in the genome of a cell. Such modifications include integration of a transgene and allelic mutations and modifications of native genes. Also provided are plants comprising a modified nucleic acid sequence compared to the native gene integrated into a targeted genomic site in the plant genome.

Abstract: Compositions and methods for conferring herbicide resistance or tolerance upon plants towards certain classes of herbicide are provided. In particular these are amine, alcohol and aminal herbicides. The compositions include nucleotide and amino acid sequences for wild-type and mutant glucosyl transferase polypeptides. The polypeptides of the invention are mutant or wild type glucosyl transferases that are capable of catalyzing the transfer of glucose to certain herbicidal structures and that, thereby, confer resistance or tolerance in plants to amine, alcohol and aminal PSII herbicides. Particularly, polypeptides of the invention include mutant or wild-type bx-type UDP glucosyl transferases.

Abstract: The invention provides three novel disarmed strains of Agrobacterium tumefaciens bacteria useful for the transformation of plants. The invention provides three engineered A. tumefaciens Chry5 strains or bacterial cells thereof which comprise the Chry5 strain chromosomal background and a disarmed pTiChry5 vector, and methods of using said bacterial strains or cells for transformation of fungal or plant cells, in particular dicot or monocot plant cells, including soybean, maize, wheat, and sugarcane cells. The invention further relates to the transgenic plants created by these methods.

Abstract: Provided here are methods of using a mutated patatin-like phospholipase II? (“pPLAII?,” renamed here MATRILINEAL) to induce haploid induction in plants, cloning a pPLAII? to induce haploid induction in plants, and genetically engineering a plant to contain a mutated pPLAII?. Also provided are methods of applying topical and spray chemicals, lipids, and RNAi molecules to plants during pollination in order to induce haploid production. Further provided are methods of chemically treating plants during pollination to induce haploids while also reducing embryo abortion and increasing seed set.

Abstract: The present invention relates to methods and compositions for modifying a target site in the genome of a plant cell. Such modifications include integration of a transgene and mutations. The present invention also relates to methods and compositions for identifying and enriching for cells which comprise a modified target site.