Abstract: Provided are a helicase mutant, a preparation method therefor, and use thereof in high-throughput sequencing. The helicase mutant has at least one cysteine on a surface of a three-dimensional structure of an amino acid sequence as set forth in SEQ ID NO: 1, the at least one cysteine being substituted with alanine. The cysteine is C133, C164, C292, C323, and/or C347. Preferably, the amino acid sequence of the helicase mutant is as set forth in SEQ ID NO: 6. The helicase mutant exhibits excellent DNA unwinding activity, good protein uniformity, reduced cross-linking interactions between protein molecules, enhanced adaptability with perforin, improved sequencing stability, as well as integrity and continuity of electrical signals. Consequently, it achieves a better sequencing performance. It can be utilized for the control and characterization of nucleic acids and applied in single-molecule nanopore sequencing.

Abstract: Techniques are directed to a solution to the problem of underutilization of features offered by an application such as a browser by making such features more visible to a user by automatically (i.e., without user intervention), surfacing a user interface element configured to invoke a feature selected from a set of available features of the application in response to determining that the feature is relevant to the content displayed by the application. In some implementations, the set of available features is derived from a prespecified association between one or more resource identifiers (e.g., web page uniform resource locators (URLs)) and at least one feature.

Abstract: An online concierge system receives an order from a user including items to obtain from a retailer for delivery to a location. A picker selects the order and obtains items from the retailer. The user selects a time interval during which items from the order are delivered to the location. To prevent the user from selecting a time interval for fulfillment the online concierge system prevents the user from selecting a time interval when a picker may be unable to obtain the items from the retailer before a closing time of the retailer. The online concierge system evaluates time intervals by subtracting a travel time for the picker travelling from the retailer to the location from a predicted fulfillment time for the order. This prevents the time for delivering items after being obtained from affecting whether a time interval may be selected.

Abstract: A comprehensive recycling method for a waste lithium iron phosphate battery relates to a waste lithium ion battery recycling technology, and particularly comprises: first selectively extracting lithium, and then using a lithium extraction residue to prepare iron phosphate, the using the lithium extraction residue to prepare the iron phosphate comprising: adding the lithium extraction residue to water to form a slurry, adding hydrochloric acid and stirring to react, so that iron is completely dissolved, performing solid-liquid separation, on the basis of iron and phosphorus contents of the obtained liquid, adding trisodium phosphate or ferric chloride, and then adding a sodium hydroxide solution to precipitate crude iron phosphate; and then performing reverse three-stage washing to remove impurities to obtain a battery iron phosphate product. The problem of environmental protection is solved and meanwhile, all of the valuable elements are recycled, and a relative cost is greatly reduced by about 25%.

Abstract: Aspects of the present disclosure address systems and methods for performing critical region resynthesis with purpose-directed candidate filtering. Data representing an integrated circuit design is accessed along with a threshold constraint defining a power consumption threshold. A first cell is selected from the integrated circuit design. A swapping candidate to evaluate for swapping with the first cell is selected. The swapping candidate comprises at least a second cell. The swapping candidate is evaluated based on a power consumption associated with the swapping candidate and the power consumption threshold. The first cell is swapped with the swapping candidate in the integrated circuit design based on a result of the evaluating. The data representing the integrated circuit design is updated based on the swapping of the first cell with the swapping candidate.

Abstract: Systems and methods for multi-turn collaboration for machine-learned inference are provided. A method can include receiving, by a computing system comprising one or more computing devices, a first input. The method can include generating, by the computing system based on the first input, structured data indicative of one or more target output properties for a machine-learned inference operation. The method can include receiving, by the computing system, one or more second inputs indicative of one or more changes to the one or more target output properties. The method can include updating, by the computing system, the structured data indicative of the one or more target output properties based on the second input to generate updated structured data. The method can include generating, by the computing system using a machine-learned model and based at least in part on the updated structured data, an output.

Abstract: One or more trained computer models are used to determine, at different stages of an order, an estimated time range for delivery of the order at an online system. The online system retrieves a set of candidate ranges of delivery times for the order. The online system applies the one or more computer models trained to predict a value of a metric for each candidate range in the set of candidate ranges, based on one or more features associated with the order. The online system selects a range of delivery times for the order from the set of candidate ranges, based on the predicted value of the metric for each candidate range. The online system causes a device of the user to display a user interface with the selected range of delivery times for the order.

Abstract: The provided is an alignment clamping fixture for automatic tailor welding of an automotive door ring and a welded seam alignment method therefor. The alignment clamping fixture includes a gripper unit and a clamp unit, where the gripper unit is connected to a working end of a robotic arm; the gripper unit includes a gripper frame, and a plurality of vision camera mechanisms and a plurality of suction cup mechanisms are mounted at a bottom of the gripper frame in a fit manner, respectively; and the clamp unit includes a clamp base, a plurality of first alignment mechanisms are mounted at a top of the clamp base in a fit manner, the first alignment mechanisms are configured to clamp a workpiece, a light source is arranged in an individual first alignment mechanism, and the light source illuminates the workpiece from a back surface of the workpiece.

Abstract: A processing method of a display screen brightness compensation includes: obtaining a sample data set of the display screen, a real environment temperature and a real image average grayscale value corresponding to the real environment temperature, the sample data set including an environment temperature sample set, a image average grayscale sample set corresponding to the environment temperature sample set and a simulated temperature sample set; performing a linear regression processing on the sample data set to generate a regression coefficient set; processing the real environment temperature and the real image average grayscale value according to the regression coefficient set to generate a temperature to be compensated of the display screen; and determining a brightness compensation parameter according to the temperature to be compensated, and performing a brightness compensation processing on the display screen according to the brightness compensation parameter.

Abstract: An electric-pulse fracturing device for hard roof of coal mine based on a self-sealing water bag and an using method thereof are provided. The device includes a power switch, a charging power supply, a current limiting protection resistor, an energy storage capacitor and a high-voltage electric pulse switch connected in sequence. The energy storage capacitor is connected with an end of a gas gap switch, an other end of the gas gap switch is connected with an assembled self-sealing water bag high-voltage electric pulse electrode structure through a high-voltage cable, and the electrode structure is fixed through an assembly rod. The self-sealing water bag high-voltage electric pulse electrode structure includes a self-sealing water bag and an electrode structure capable of focusing shock wave energy, and the number of the self-sealing water bag high-voltage electric pulse electrode structure depends on fracturing effect.

Abstract: Various embodiments provide for modifying a circuit design based on timing tradeoff, which can be part of an electronic design automation (EDA) system. For instance, some embodiments use a return on investment (ROI) concept to determine one or more thresholds for how much area or power of a circuit design should be available for use (e.g., sacrificed) in order to obtain an improvement in slack by a given transformation.

Abstract: Embodiments relate to determining an availability of a service option for delivery of an order placed with an online system. The online system receives an order placed with the online system. The online system accesses a computer model trained to predict a value of metric for an order placed with the online system. The online system applies the computer model to predict the value of the metric for the order. The online system determines which service option of a plurality of service options of the online system is available for delivery of the order, based at least in part on the predicted value of the metric and a threshold. The online system causes the device of the user to display an availability of the determined service option for delivery of the order.

Abstract: The invention provides a vehicle's brake system and a method for braking a vehicle. The brake system comprises at least two control modules and a plurality of electromechanical brake devices. Each electromechanical brake device comprises a wheel end controller, a motor actuator and a friction brake driven by the motor actuator. Each control module receives a brake demand from a brake demand generating device and sends a control signal to each wheel end controller according to the brake demand. Each wheel end controller only responds to the control signals when the control signals received from the two control modules are consistent.

Abstract: An online concierge system provides arrival prediction services for a user placing an order to be retrieved by a shopper. An order may have a predicted arrival time predicted by a model that may err under some conditions. To reduce the likelihood of providing the predicted arrival time (and related services) when the arrival time may be incorrect, the prediction model and related services are throttled (e.g., selectively provided) based on one or more predicted delivery metrics, which may include a time to accept the order by a shopper and a predicted portion of late orders that will be delivered past the respective predicted arrival times. The predicted delivery metrics are compared with thresholds and the result of the comparison used to selectively provide, or not provide, the predicted delivery services.

Abstract: An online concierge system provides arrival prediction services for a user placing an order to be retrieved by a shopper. An order may have a predicted arrival time predicted by a model that may err under some conditions. To reduce the likelihood of providing the predicted arrival time (and related services) when the arrival time may be incorrect, the prediction model and related services are throttled (e.g., selectively provided) based on one or more predicted delivery metrics, which may include a time to accept the order by a shopper and a predicted portion of late orders that will be delivered past the respective predicted arrival times. The predicted delivery metrics are compared with thresholds and the result of the comparison used to selectively provide, or not provide, the predicted delivery services.

Abstract: An online concierge system receives two types of orders, one of which requires fulfillment in a specific time interval, while the other can be fulfilled anytime up to a specific time interval. A machine learning model, trained on historical data about available shoppers in discrete time intervals, is used to predict how many shoppers will be available to fulfill orders in each time interval. For each time interval, the system retrieves the relevant orders of both types and creates candidate groups including orders of both types. For each group, the system determines a fulfillment cost based on items in the orders. The candidate group with the lowest cost is selected, and the orders in the selected group are sent to devices of available shoppers in that interval, prompting the shoppers to view and fulfill the orders.

Abstract: An electric-pulse fracturing device for hard roof of coal mine based on a self-sealing water bag and an using method thereof are provided. The device includes a power switch, a charging power supply, a current limiting protection resistor, an energy storage capacitor and a high-voltage electric pulse switch connected in sequence. The energy storage capacitor is connected with an end of a gas gap switch, an other end of the gas gap switch is connected with an assembled self-sealing water bag high-voltage electric pulse electrode structure through a high-voltage cable, and the electrode structure is fixed through an assembly rod. The self-sealing water bag high-voltage electric pulse electrode structure includes a self-sealing water bag and an electrode structure capable of focusing shock wave energy, and the number of the self-sealing water bag high-voltage electric pulse electrode structure depends on fracturing effect.

Abstract: The disclosure provides a compensation method, system, device and medium of a IR Drop of a display panel. The compensation method includes: constructing a IR Drop model of the display panel based on a distribution of equivalent resistances between adjacent pixels in the display panel; constructing a vector matrix equation of the IR Drop according to the IR Drop model; performing an equivalent processing on the vector matrix equation of the IR Drop to form a plurality of equivalent sub-matrices; iteratively solving IR Drop data of each node in each equivalent sub-matrix; and performing a voltage compensation on each pixel in the display panel according to the IR Drop data. The disclosure may realize a point-to-point accurate compensation of all nodes in the display panel, and improves a problem of uneven brightness of a high-resolution display panel caused by the IR Drop.

Abstract: One or more trained computer models are used to determine, at different stages of an order, an estimated time range for delivery of the order at an online system. The online system retrieves a set of candidate ranges of delivery times for the order. The online system applies the one or more computer models trained to predict a value of a metric for each candidate range in the set of candidate ranges, based on one or more features associated with the order. The online system selects a range of delivery times for the order from the set of candidate ranges, based on the predicted value of the metric for each candidate range. The online system causes a device of the user to display a user interface with the selected range of delivery times for the order.