Patents by Inventor Zicai Liang

Zicai Liang has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20250057870
    Abstract: Provided are siRNA for inhibiting an expression level of a RPTOR gene and regulating and controlling the activity of mTORC1, and a pharmaceutical composition and conjugate containing the siRNA. The siRNA includes a sense strand and an antisense strand; each nucleotide in the siRNA is independently a modified or unmodified nucleotide. The sense strand includes a nucleotide sequence I, the length of the nucleotide sequence I is equal to the length of a nucleotide sequence as shown in SEQ ID NO: 1, with no more than three nucleotide differences; the antisense strand includes a nucleotide sequence II, the length of the nucleotide sequence II is equal to the length of a nucleotide sequence as shown in SEQ ID NO: 2, with no more than three nucleotide differences.
    Type: Application
    Filed: December 19, 2022
    Publication date: February 20, 2025
    Applicant: SUZHOU RIBO LIFE SCIENCE CO., LTD.
    Inventors: Zicai LIANG, Hongyan ZHANG, Shan GAO, Xuefeng DING
  • Publication number: 20240325428
    Abstract: An siRNA capable of inhibiting expression of apolipoprotein C3(APOC3) gene, comprising a sense strand and an antisense strand; the sense strand and the antisense strand respectively comprise a nucleotide sequence I and a nucleotide sequence II consisting of 19 modified or unmodified nucleotides, the nucleotide sequence I and the nucleotide sequence II are at least partially reversely complementary to form a double-stranded region, and the nucleotide sequence II is at least partially reversely complementary to a nucleotide sequence in an mRNA expressed by APOC3 gene; in a direction from 5? end to 3? end, at least one of the 3rd to 6th nucleotides of the nucleotide sequence II is a stabilizing modified nucleotide. The siRNA and a pharmaceutical composition and an siRNA conjugate containing same can effectively treat and/or prevent diseases or disorders related to expression of APOC3 gene, and have a significantly reduced off-target effect.
    Type: Application
    Filed: June 30, 2022
    Publication date: October 3, 2024
    Applicant: SUZHOU RIBO LIFE SCIENCE CO., LTD.
    Inventors: Zicai LIANG, Hongyan ZHANG, Shan GAO
  • Publication number: 20240309378
    Abstract: A double-stranded oligonucleotide capable of regulating the expression level of a target gene includes a sense strand and an antisense strand. The sense strand and the antisense strand each includes nucleotide sequence I or nucleotide sequence II consisting of 19 modified or unmodified nucleotides, the nucleotide sequence I and the nucleotide sequence II are at least partially reverse complementary to form a double-stranded region, and the nucleotide sequence II is at least partially reverse complementary to a nucleotide sequence in an mRNA expressed by the target gene; and according to the direction from the 5? end to the 3? end, at least one of the 3rd to 6th nucleotides of the nucleotide sequence II is a stabilizing modified nucleotide, and none of the nucleotides other than the 3rd to 9th nucleotides in the nucleotide sequence II are stabilizing modified nucleotides.
    Type: Application
    Filed: July 13, 2022
    Publication date: September 19, 2024
    Applicant: SUZHOU RIBO LIFE SCIENCE CO., LTD.
    Inventors: Zicai LIANG, Hongyan ZHANG, Shan GAO, Haitao LI
  • Patent number: 9738894
    Abstract: The present invention is directed to novel double-stranded short interfering (siRNA) analogs comprising locked nucleic acid (LNA) monomers. Such compounds induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference (RNAi). The compounds disclosed herein has improved properties compared to non-modified siRNAs and may, accordingly, be useful as therapeutic agents, e.g., in the treatment of various cancer forms. More particularly, the present invention is directed to siRNA analogs comprising a sense strand and an antisense strand, wherein each strand comprises 12-35 nucleotides and wherein the siRNA analogs comprise at least one locked nucleic acid (LNA) monomer.
    Type: Grant
    Filed: March 28, 2016
    Date of Patent: August 22, 2017
    Assignee: Roche Innovation Center Copenhagen A/S
    Inventors: Joacim Elmen, Claes Wahlestedt, Zicai Liang, Anders M. Sorensen, Henrik Orum, Troels Koch
  • Patent number: 9567364
    Abstract: Nucleotide and/or oligonucleotide represented by formula (1) and the liquid phase synthesis process thereof. The present invention provides a liquid phase synthesis process for preparing a nucleotide and/or an oligonucleotide, comprising a process for combining the nucleotide and/or oligonucleotide protective groups, in which, under the condition that the 2?-hydroxyl group is protected by a group with a sterically hindered silane structure, the 3? phosphate group(s) of the nucleotide and/or oligonucleotide is/are directly protected by (a)?-cyanoethyl group(s), and after the ?-cyanoethyl group(s) is/are removed, the resulting product can directly participate in the next cycle of synthesis, wherein the synthesis reaction is carried out in a reaction flask or reaction kettle, without being limited by a solid carrier or synthesizer, so that the large scale preparation of oligonucleotides can be achieved.
    Type: Grant
    Filed: July 20, 2011
    Date of Patent: February 14, 2017
    Assignee: Suzhou Ribo Life Sciene Co., Ltd.
    Inventors: Zhen Xi, Zicai Liang, Jinyu Huang
  • Publication number: 20170009237
    Abstract: The present invention is directed to novel double-stranded short interfering (siRNA) analogues comprising locked nucleic acid (LNA) monomers. Such compounds induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference (RNAi). The compounds disclosed herein has improved properties compared to non-modified siRNAs and may, accordingly, be useful as therapeutic agents, e.g., in the treatment of various cancer forms. More particularly, the present invention is directed to siRNA analogues comprising a sense strand and an antisense strand, wherein each strand comprises 12-35 nucleotides and wherein the siRNA analogues comprise at least one locked nucleic acid (LNA) monomer.
    Type: Application
    Filed: March 28, 2016
    Publication date: January 12, 2017
    Inventors: Joacim Elmen, Claes Wahlestedt, Zicai Liang, Anders M. Sorensen, Henrik Orum, Troels Koch
  • Patent number: 9297010
    Abstract: The present invention is directed to novel double-stranded short interfering (siRNA) analogs comprising locked nucleic acid (LNA) monomers. Such compounds induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference (RNAi). The compounds disclosed herein has improved properties compared to non-modified siRNAs and may, accordingly, be useful as therapeutic agents, e.g., in the treatment of various cancer forms. More particularly, the present invention is directed to siRNA analogs comprising a sense strand and an antisense strand, wherein each strand comprises 12-35 nucleotides and wherein the siRNA analogs comprise at least one locked nucleic acid (LNA) monomer.
    Type: Grant
    Filed: February 11, 2014
    Date of Patent: March 29, 2016
    Assignee: Roche Innovation Center Copenhagen A/S
    Inventors: Joacim Elmen, Claes Wahlestedt, Zicai Liang, Anders M. Sorensen, Henrik Orum, Troels Koch
  • Patent number: 8846895
    Abstract: The present invention discloses preparation and application of double-stranded RNA molecules stable in mammalian body fluids. The mammalian-body-fluid-stable RNA molecules disclosed in the present invention are comprised of only unmodified nucleotides. For the first time, the present invention discloses the applications of mammalian-body-fluid-stable RNA molecules for immunotherapy and siRNA drug development.
    Type: Grant
    Filed: March 29, 2011
    Date of Patent: September 30, 2014
    Assignee: Biomics Biotechnologies Co. Ltd.
    Inventors: Quan Du, Zicai Liang
  • Publication number: 20140235844
    Abstract: The present invention is directed to novel double-stranded short interfering (siRNA) analogues comprising locked nucleic acid (LNA) monomers. Such compounds induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference (RNAi). The compounds disclosed herein has improved properties compared to non-modified siRNAs and may, accordingly, be useful as therapeutic agents, e.g., in the treatment of various cancer forms. More particularly, the present invention is directed to siRNA analogues comprising a sense strand and an antisense strand, wherein each strand comprises 12-35 nucleotides and wherein the siRNA analogues comprise at least one locked nucleic acid (LNA) monomer.
    Type: Application
    Filed: February 11, 2014
    Publication date: August 21, 2014
    Applicant: Santaris Pharma A/S
    Inventors: Joacim Elmen, Claes Wahlestedt, Zicai Liang, Anders M. Sorensen, Henrik Orum, Troels Koch
  • Patent number: 8754058
    Abstract: Inhibitors that can inhibit expression of FAM3B gene to reduce the levels of expression products, or can combine the expression products to reduce the activity of promoting lipid synthesis of FAM3B gene product are provided, wherein the inhibitors are one or more inhibitors selected from the group consisting of small interfering RNAs, antisense oligonucleotides, antibodies against FAM3B proteins and active organic compounds. Cells, vectors or inhibitor compositions, comprising such inhibitors, methods for inhibiting expression of FAM3B gene or inhibiting the activity of promoting lipid synthesis of FAM3B gene product using the inhibitors are provided. Methods for treating diseases mediated by expression of FAM3B gene using such inhibitors and uses of the inhibitors in preparing pharmaceuticals for preventing and/or treating the disease mediated by FAM3B gene expression are also provided.
    Type: Grant
    Filed: November 26, 2010
    Date of Patent: June 17, 2014
    Assignee: Suzhou Ribo Life Science Company, Ltd.
    Inventors: Youfei Guan, Zicai Liang
  • Patent number: 8653252
    Abstract: The present invention is directed to novel double-stranded short interfering (siRNA) analogues comprising locked nucleic acid (LNA) monomers. Such compounds induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference (RNAi). The compounds disclosed herein has improved properties compared to non-modified siRNAs and may, accordingly, be useful as therapeutic agents, e.g., in the treatment of various cancer forms. More particularly, the present invention is directed to siRNA analogues comprising a sense strand and an antisense strand, wherein each strand comprises 12-35 nucleotides and wherein the siRNA analogues comprise at least one locked nucleic acid (LNA) monomer.
    Type: Grant
    Filed: March 22, 2004
    Date of Patent: February 18, 2014
    Assignee: Santaris Pharma A/S
    Inventors: Joachim Elmén, Claes Wahlestedt, Zicai Liang, Anders Malling Sørensen, Henrik Ørum, Troels Koch
  • Patent number: 8563710
    Abstract: The present invention relates to a modified oligonucleotide, its preparation and application. The invention eables stabilizing the oligonucleotide by introducing a relatively small amount of modified nucleotide at specific UA/UA and/or CA/UG and/or UG/CA site of the oligonucleotide, therefore to decrease the modification-related cytotoxicity and compromising effects on the biological activity.
    Type: Grant
    Filed: March 30, 2010
    Date of Patent: October 22, 2013
    Assignee: Biomics Biotechnologies Co., Ltd.
    Inventor: Zicai Liang
  • Publication number: 20130123482
    Abstract: Nucleotide and/or oligonucleotide represented by formula (1) and the liquid phase synthesis process thereof. The present invention provides a liquid phase synthesis process for preparing a nucleotide and/or an oligonucleotide, comprising a process for combining the nucleotide and/or oligonucleotide protective groups, in which, under the condition that the 2?-hydroxyl group is protected by a group with a sterically hindered silane structure, the 3? phosphate group(s) of the nucleotide and/or oligonucleotide is/are directly protected by (a)?-cyanoethyl group(s), and after the ?-cyanoethyl group(s) is/are removed, the resulting product can directly participate in the next cycle of synthesis, wherein the synthesis reaction is carried out in a reaction flask or reaction kettle, without being limited by a solid carrier or synthesizer, so that the large scale preparation of oligonucleotides can be achieved.
    Type: Application
    Filed: July 20, 2011
    Publication date: May 16, 2013
    Applicant: SUZHOU RIBO LIFE SCIENCE CO., LTD
    Inventors: Zhen Xi, Zicai Liang, Jinyu Huang
  • Publication number: 20130065940
    Abstract: The present invention discloses preparation and application of double-stranded RNA molecules stable in mammalian body fluids. The mammalian-body-fluid-stable RNA molecules disclosed in the present invention are comprised of only unmodifide nucleotides. For the first time, the present invention discloses the applications of mammalian-body-fluid-stable RNA molecules for immunotherapy and siRNA drug development.
    Type: Application
    Filed: March 29, 2011
    Publication date: March 14, 2013
    Inventors: Quan Du, Zicai Liang
  • Publication number: 20130046006
    Abstract: Inhibitors that can inhibit expression of FAM3B gene to reduce the levels of expression products, or can combine the expression products to reduce the activity of promoting lipid synthesis of FAM3B gene product are provided, wherein the inhibitors are one or more inhibitors selected from the group consisting of small interfering RNAs, antisense oligonucleotides, antibodies against FAM3B proteins and active organic compounds. Cells, vectors or inhibitor compositions, comprising such inhibitors, methods for inhibiting expression of FAM3B gene or inhibiting the activity of promoting lipid synthesis of FAM3B gene product using the inhibitors are provided. Methods for treating diseases mediated by expression of FAM3B gene using such inhibitors and uses of the inhibitors in preparing pharmaceuticals for preventing and/or treating the disease mediated by FAM3B gene expression are also provided.
    Type: Application
    Filed: November 26, 2010
    Publication date: February 21, 2013
    Applicant: Suzhou Ribo Life Science Co., Ltd.
    Inventors: Youfei Guan, Zicai Liang
  • Publication number: 20120088815
    Abstract: The present invention relates to a modified oligonucleotide, its preparation and application. The invention enables stabilizing the oligonucleotide by introducing a relatively small amount of modified nucleotide at specific UA/UA and/or CA/UG and/or UG/CA site of the oligonucleotide, therefore to decrease the modification-related cytotoxicity and compromising effects on the biological activity.
    Type: Application
    Filed: March 30, 2010
    Publication date: April 12, 2012
    Applicant: BIOMICS BIOTECHNOLOGIES CO. LTD
    Inventor: Zicai Liang
  • Publication number: 20100317714
    Abstract: The present invention provides a complex molecule interfering the expression of target genes and the methods for preparing the complex molecule, wherein the complex molecule contains two siRNA strands X1 and X2 having at least 80% complementarity, the 5? end of X1 and 3? end of X2 are linked through non-nucleic acid molecule L1, the 5? end of X2 and 3? end of X1 are linked through non-nucleic acid molecule L2. Since both 5? and 3? ends of two siRNA strands X1 and X2 of the complex molecule according to the present invention are linked through non-nucleic acid molecules, it is not easy to unwind and degraded for the siRNA strands, and therefore the chemical stability of siRNA and the remaining time in the blood are greatly improved. After being administered, the Dicer enzyme in the cells is utilized to release the locked siRNAs from the complex molecules, and after unwinding, the antisense strand of the siRNA is released from the double-stranded siRNA to inhibit the expression of the target genes.
    Type: Application
    Filed: November 28, 2008
    Publication date: December 16, 2010
    Applicant: SUZHOU RIBO LIFE SCIENCE CO., LTD
    Inventors: Zhen Xi, Zicai Liang, Liqiang Cao, Junbin Zhang, Jinyu Huang
  • Publication number: 20100009856
    Abstract: This invention relates to DNA libraries based on plasmid or viral vectors that can express double-stranded RNA of 10-30 base pairs in length with all possible sequences, where each of the double stranded RNA is formed by a single RNA molecule in the form of hairpin, or formed by two separate RNA molecules with different 3?-overhangs. Each single member in such a DNA library encodes all components of a double stranded RNA as specified above. Such a library can be used in screening for double stranded RNA species that can induce a given phenotype without prior knowledge of their target genes. This invention further relates to a method to generate such a DNA library.
    Type: Application
    Filed: June 23, 2003
    Publication date: January 14, 2010
    Applicant: Sinogenomax Sompany LTD.
    Inventors: Zicai Liang, Hong-Yan Zhang, Meihong Cheng, Yan Shen
  • Publication number: 20080249039
    Abstract: The present invention is directed to modified siRNA which are significantly impaired in their ability to support cleavage of mRNA when incorporated into a RISC complex. Such modified siRNA may be useful as therapeutic agents, e.g., in the treatment of various cancer forms. More particularly, the modified siRNA comprises a sense strand and an antisense strand, wherein the sense strand contains a modified RNA nucleotide in at least one of positions 8-14, calculated from the 5?-end.
    Type: Application
    Filed: January 28, 2005
    Publication date: October 9, 2008
    Applicant: SANTARIS PHARMA A/S
    Inventors: Joacim Elmen, Claes Wahlestedt, Zicai Liang, Henrik Orum, Troels Koch
  • Publication number: 20070191294
    Abstract: The present invention is directed to novel double-stranded short interfering (siRNA) analogues comprising locked nucleic acid (LNA) monomers. Such compounds induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference (RNAi). The compounds disclosed herein has improved properties compared to non-modified siRNAs and may, accordingly, be useful as therapeutic agents, e.g., in the treatment of various cancer forms. More particularly, the present invention is directed to siRNA analogues comprising a sense strand and an antisense strand, wherein each strand comprises 12-35 nucleotides and wherein the siRNA analogues comprise at least one locked nucleic acid (LNA) monomer.
    Type: Application
    Filed: March 22, 2004
    Publication date: August 16, 2007
    Inventors: Joacim Elmen, Claes Wahlestedt, Zicai Liang, Anders Sorensen, Henrik Orum, Troels Koch