MOLECULAR MODIFICATION ASSAYS
Systems for detecting molecular modifications and the presence and/or activity of enzymes and/or other agents involved in facilitating or otherwise regulating such modifications.
This application is a continuation of U.S. patent application Ser. No. 11/241,872, filed Jun. 30, 2005, now U.S. Pat. No. 7,632,651.
U.S. patent application Ser. No. 11/241,872, in turn, is a continuation-in-part of the following U.S. patent applications: Ser. No. 10/746,797, filed Dec. 23, 2003; Ser. No. 11/146,553, filed Jun. 6, 2005; and Ser. No. 10/957,332, filed Sep. 30, 2004. The '872 application also was based upon and claimed the benefit under 35 U.S.C. §119(e) of the following U.S. provisional patent applications: Ser. No. 60/615,308, filed Sep. 30, 2004; and Ser. No. 60/683,377, filed May 20, 2005.
U.S. patent application Ser. No. 11/146,553, in turn, is a continuation-in-part of U.S. patent application Ser. No. 10/746,797, filed Dec. 23, 2004. The '553 application also was based upon and claimed the benefit under 35 U.S.C. §119(e) of the following U.S. provisional patent applications: Ser. No. 60/577,079, filed Jun. 4, 2004; Ser. No. 60/602,712, filed Aug. 18, 2004; and Ser. No. 60/615,308, filed Sep. 30, 2004.
U.S. patent application Ser. No. 10/746,797, in turn, is a continuation-in-part of U.S. patent application Ser. No. 09/844,655, filed Apr. 27, 2001. The '797 application also was based upon and claimed the benefit under 35 U.S.C. §119(e) of the following U.S. provisional patent applications: Ser. No. 60/436,725, filed Dec. 26, 2002; and Ser. No. 60/507,006, filed Sep. 29, 2003.
U.S. patent application Ser. No. 09/844,655, in turn, is a continuation-in-part of the following patent applications: PCT Patent Application Serial No. PCT/US00/16025, filed Jun. 9, 2000; and U.S. patent application Ser. No. 09/596,444, filed Jun. 19, 2000. The '655 application also was based upon and claimed the benefit under 35 U.S.C. §119(e) of the following U.S. provisional patent applications: Ser. No. 60/200,594, filed Apr. 28, 2000; Ser. No. 60/223,642, filed Aug. 8, 2000; and Ser. No. 60/241,032, filed Oct. 17, 2000.
PCT Patent Application Serial No. PCT/US00/16025, in turn, is a continuation-in-part of U.S. patent application Ser. No. 09/349,733, filed Jul. 8, 1999. The '16025 application also was based upon and claimed the benefit under 35 U.S.C. §119(e) of the following U.S. provisional patent applications: Ser. No. 60/138,311, filed Jun. 9, 1999; Ser. No. 60/138,438, filed Jun. 10, 1999; and Ser. No. 60/200,594, filed Apr. 28, 2000.
U.S. patent application Ser. No. 09/596,444, in turn, is a continuation-in-part of the following patent applications: U.S. patent application Ser. No. 08/929,095, filed Sep. 15, 1997; and PCT Patent Application Serial No. PCT/US00/16025, with priority claims as indicated above.
U.S. patent application Ser. No. 09/349,733, in turn, is based upon and claims the benefit under 35 U.S.C. §119(e) of U.S. Provisional Patent Application Ser. No. 60/092,203, filed Jul. 9, 1998.
U.S. patent application Ser. No. 10/957,332, in turn, is based upon and claims the benefit under 35 U.S.C. §119(e) of U.S. Provisional Patent Application Ser. No. 60/507,569, filed Sep. 30, 2003.
Each of the above-identified U.S., PCT, and provisional patent applications is incorporated herein by reference in its entirety for all purposes.
CROSS-REFERENCES TO OTHER MATERIALSThis application incorporates by reference the following U.S. patents: U.S. Pat. No. 5,843,378, issued Dec. 1, 1998; No. 6,965,381, issued Oct. 12, 1999; No. 6,071,748, issued Jun. 6, 2000; and No. 6,097,025, issued Aug. 1, 2000.
This application also incorporates by reference the following U.S. patent applications: Ser. No. 08/840,553, filed Apr. 14, 1997; Ser. No. 09/118,141, filed Jul. 16, 1998; Ser. No. 09/144,578, filed Aug. 31, 1998; Ser. No. 09/156,318, filed Sep. 18, 1998; Ser. No. 09/478,819, filed Jan. 5, 2000; Ser. No. 09/626,208, filed Jul. 26, 2000; Ser. No. 09/643,221, filed Aug. 18, 2000; Ser. No. 09/710,061, filed Nov. 10, 2000; Ser. No. 09/722,247, filed Nov. 24, 2000; Ser. No. 09/733,370, filed Dec. 8, 2000; Ser. No. 09/759,711, filed Jan. 12, 2001; Ser. No. 09/765,869, filed Jan. 19, 2001; Ser. No. 09/765,874, filed Jan. 19, 2001; Ser. No. 09/766,131, filed Jan. 19, 2001; Ser. No. 09/767,316, filed Jan. 22, 2001; Ser. No. 09/767,434, filed Jan. 22, 2001; Ser. No. 09/767,579, filed Jan. 22, 2001; Ser. No. 09/767,583, filed Jan. 22, 2001; Ser. No. 09/768,661, filed Jan. 23, 2001; Ser. No. 09/768,742, filed Jan. 23, 2001; Ser. No. 09/768,765, filed Jan. 23, 2001; Ser. No. 09/770,720, filed Jan. 25, 2001; Ser. No. 09/770,724, filed Jan. 25, 2001; Ser. No. 09/777,343, filed Feb. 5, 2001; Ser. No. 09/813,107, filed Mar. 19, 2001; Ser. No. 09/815,932, filed Mar. 23, 2001; and Ser. No. 09/836,575, filed Apr. 16, 2001.
This application also incorporates by reference the following PCT patent applications: Serial No. PCT/US98/23095, filed Oct. 30, 1998; Serial No. PCT/US99/01656, filed Jan. 25, 1999; Serial No. PCT/US99/03678, filed Feb. 19, 1999; and Serial No. PCT/US99/08410, filed Apr. 16, 1999.
This application also incorporates by reference the following U.S. provisional patent applications: Ser. No. 60/092,203, filed Jul. 9, 1998; Ser. No. 60/094,275, filed Jul. 27, 1998; Ser. No. 60/094,276, filed Jul. 27, 1998; Ser. No. 60/094,306, filed Jul. 27, 1998; Ser. No. 60/100,817, filed Sep. 18, 1998; Ser. No. 60/100,951, filed Sep. 18, 1998; Ser. No. 60/104,964, filed Oct. 20, 1998; Ser. No. 60/114,209, filed Dec. 29, 1998; Ser. No. 60/116,113, filed Jan. 15, 1999; Ser. No. 60/117,278, filed Jan. 26, 1999; Ser. No. 60/119,884, filed Feb. 12, 1999; Ser. No. 60/121,229, filed Feb. 23, 1999; Ser. No. 60/124,686, filed Mar. 16, 1999; Ser. No. 60/125,346, filed Mar. 19, 1999; Ser. No. 60/126,661, filed Mar. 29, 1999; Ser. No. 60/130,149, filed Apr. 20, 1999; Ser. No. 60/132,262, filed May 3, 1999; Ser. No. 60/132,263, filed May 3, 1999; Ser. No. 60/135,284, filed May 21, 1999; Ser. No. 60/136,566, filed May 28, 1999; Ser. No. 60/138,737, filed Jun. 11, 1999; Ser. No. 60/138,893, filed Jun. 11, 1999; Ser. No. 60/142,721, filed Jul. 7, 1999; Ser. No. 60/178,026, filed Jan. 26, 2000; Ser. No. 60/222,222, filed Aug. 1, 2000; Ser. No. 60/244,012, filed Oct. 27, 2000; Ser. No. 60/250,681, filed Nov. 30, 2000; Ser. No. 60/250,683, filed Nov. 30, 2000; Ser. No. 60/267,639, filed Feb. 10, 2001; Ser. No. 60/369,704, filed Apr. 2, 2002; and Ser. No. 60/554,766, filed Mar. 19, 2004.
This application also incorporates by reference the following publications: Richard P. Haugland, Handbook of Fluorescent Probes and Research Chemicals (6th ed. 1996); Joseph R. Lakowicz, Principles of Fluorescence Spectroscopy (2nd Edition 1999); and Bob Sinclair, Everything's Great When It Sits on a Chip: A Bright Future for DNA Arrays, 13 T
The physiological modification of molecules and supramolecular assemblies plays a major role in the structure and regulation of biological systems. These modifications may include “local” modifications, such as phosphorylation, cyclization, prenylation, carboxylation, glycosylation, acylation, and/or sulfonation of amino acids, nucleotides, and/or lipids, among others, as well as the reversal of these modifications. These modifications also may include “global” modifications, among others, such as cleavage and/or ligation of proteins, nucleic acids, and/or lipids, in which molecules are split into two or more fragments or spliced to one or more other molecules, respectively.
The prevalence and significance of molecular modifications make it particularly likely that errors in modifications and/or errors in the regulation of such modifications will lead to disease and/or other pathologies. Therefore, there is intense interest both in characterizing molecular modifications and in understanding their regulation. There also is intense interest in identifying and/or characterizing activating and/or inhibitory drugs to modulate molecular modifications. This interest, in turn, has created a demand for assays of molecular modifications and/or their effects. Unfortunately, standard assays have a number of shortcomings, including the use of radioactive labels (with their attendant safety issues), binding partners that are excessively specific to the substrate or product (making them difficult to use with more than one or unknown substrates or products), and/or slow time courses and unstable endpoints (that require precise timing of assay readouts), among others. Thus, there is a significant need for molecular modification assays that are both safe and simple.
SUMMARYThe present teachings relate to systems for detecting molecular modifications and the presence and/or activity of enzymes and/or other agents involved in facilitating or otherwise regulating such modifications.
The various technical terms used herein generally have the meanings that are commonly recognized by those skilled in the art. However, some terms may have additional and/or alternative meanings, as described below, and/or as described in the Definitions sections of the patents and patent applications listed above under Cross-References, which are incorporated herein by reference, particularly Ser. No. 10/746,797, filed Dec. 23, 2003 (e.g., for phosphate modifications); Ser. No. 60/554,766, filed Mar. 19, 2004 (e.g., for protease and ligation modifications); Ser. No. 10/957,332, filed Sep. 30, 2004 (e.g., for prenyl transfer modifications); and Ser. No. 11/146,553, filed Jun. 6, 2005 (e.g., for lipid modifications):
Association—a detectable connection, proximity, and/or binding between a first partner (a moiety, molecule, or group of molecules) and a second partner (moiety, molecule, or group of molecules). The association may include covalent linkages, higher affinity (e.g., specific) binding, lower affinity binding, and/or relatively nonspecific binding, among others.
Cyclization/decyclization—the formation or degradation of a ring connecting a phosphate group and a nucleoside in a nucleotide. A common cyclization forms cAMP and cGMP from ATP and GTP, respectively, by removing two phosphate groups from the nucleotide triphosphates and joining the “free” end of the remaining phosphate group to the sugar in the remaining nucleotide monophosphate. A common decyclization reaction degrades the ring to form noncyclized AMP and noncyclized GMP from cAMP and cGMP, respectively.
Immunoglobulin—a group of typically large glycoproteins secreted by plasma cells in vertebrates that function as antibodies in the immune response by binding to specific antigens.
Luminescent—capable of, suitable for, or exhibiting luminescence, which is the emission of light by sources other than a hot, incandescent body. Luminescence is caused by electronic transitions within a luminescent substance (or luminophore) from more energetic to less energetic states. Among several types are chemiluminescence, electrochemiluminescence, electroluminescence, photoluminescence, and triboluminescence, which are produced by chemical reactions, electrochemical reactions, electric discharges, absorption of light, and the rubbing or crushing of crystals, respectively. Molecules may be intrinsically and/or extrinsically luminescent, meaning that they are luminescent on their own or luminescent due to covalent and/or noncovalent association with another molecule that is luminescent. Exemplary luminescent molecules and mechanisms for producing luminescent molecules, as well as exemplary energy transfer pairs, are described in U.S. patent application Ser. No. 09/815,932, filed Mar. 23, 2001; and in Richard P. Haugland, Handbook of Fluorescent Probes and Research Chemicals (6th ed. 1996), each of which is incorporated herein by reference.
Nucleotide—a compound comprising a nucleoside and a phosphate group, some of which function as cell regulators and some of which function as the basic constituent of DNA and RNA. A nucleoside in turn is a compound comprising a sugar, such as ribose or deoxyribose, and a purine or pyrimidine base, such as adenine, cytosine, guanine, thymine, or uracil. Nucleotides are named according to the identities of their constituent bases and sugars, the number of their constituent phosphates, and the presence or absence of cyclization. Suitable nucleotides are listed in the following table:
Phosphorylation/dephosphorylation—the introduction or removal of a phosphate group to or from an organic molecule such as a polypeptide. Phosphorylation is a versatile posttranslational modification that is a recurrent theme for regulation of enzyme activity and signal transduction pathways.
Polypeptide—a polymer comprising two or more amino acid residues linked together by covalent bonds, typically from amino end to carboxyl end by peptide bonds, and/or modifications or complexes thereof. Polypeptides generally include peptides, proteins, and/or protein segments, among others. Here, peptide generally refers to smaller polypeptides (e.g., less than about 100, 50, 20, or 10 amino acids, among others), and protein generally refers to larger polypeptides, and/or complexes thereof, possibly modified by other organic or inorganic conjugated chemical groups, such as phosphates, sugars, luminescent labels, and/or so on. Polypeptides may include unbranched chains and/or branched chains, among others. Exemplary amino acids are listed in Table 2:
Specific binding—binding to a specific partner to the general exclusion of binding to most other potential partners. Specific binding can be characterized by a dissociation constant. Generally, dissociation constants for specific binding range from 10−4 M to 10−12 M and lower, and preferred dissociation constants for specific binding range from 10−8 or 10−9 M to 10−12 M and lower.
DETAILED DESCRIPTIONThe present teachings relate to systems for detecting molecular modifications and the presence and/or activity of enzymes and/or other agents involved in facilitating or otherwise regulating such modifications. The modifications may include, among others, (1) phosphate modifications such as the phosphorylation and dephosphorylation of molecules such as polypeptides and lipids, and the cyclization and decyclization of molecules such as nucleotides, (2) prenyl modifications such as the prenylation and deprenylation of molecules such as polypeptides, and/or (3) the cleavage and/or degradation of molecules such as polypeptides and lipids. The enzymes may include, among others, enzymes involved in performing and/or regulating phosphorylation, dephosphorylation, prenylation, cyclization, decyclization, cleavage, and ligation modifications, such as kinases, phosphatases, prenyl transferases, cyclases, phosphodiesterases (PDEs), proteases, and ligases, respectively. The systems may include, among others, luminescence assays, such as assays detecting luminescence energy transfer, luminescence polarization, and/or luminescence intensity. The systems provided by the present teachings may be useful in a variety of applications, including, without limitation, life science research, drug research, accelerated drug discovery, assay development, and high-throughput screening.
Further aspects of the present teachings are described in the following sections, including, among others, (I) cell-signaling pathways, (II) overview, (III) assays, and (IV) examples.
I. CELL-SIGNALING PATHWAYSThe systems described herein may be used for any suitable purpose(s), including, among others, (1) detection of the presence and/or state of modification of a composition, such as an enzyme substrate or enzyme product, (2) the presence and/or activity of an enzyme, for example, that might act on an enzyme substrate to generate an enzyme product, and/or (3) the presence and/or activity of a modulator of enzyme activity. These assays may be performed on any suitable system(s), including biological systems, which may be studied in vitro and/or in vivo. This section describes, without limitation, exemplary cell-signaling pathways that are suitable for analysis using the disclosed assays.
Signaling cells 102 are cells capable of producing a signal (substance) that can effect a specific response in another (target) cell. The signaling cells may be components of an endocrine, paracrine, or nervous system. The endocrine system is an organism-wide control system that regulates body function using hormones released by endocrine organs into the bloodstream. The endocrine organs include the pituitary gland, thyroid gland, parathyroid glands, adrenal glands, thymus gland, pineal body, pancreas, ovaries, testes, and kidneys. The paracrine system is a local control system that regulates nearby cells using local mediators released into the extracellular medium. The nervous system is a specialized control system that regulates specific cells using electrical impulses and neurotransmitters.
Signal substances 104a,b are substances through which a signaling cell may communicate with target cells, evoking a specific response. Signal substances may act as hormones, local mediators, and/or neurotransmitters, among others. Signal substances may take the form of proteins, small peptides, amino acids, nucleotides, steroids (e.g., cortisol, steroid sex hormones, vitamin D), retinoids, fatty acid derivatives, and dissolved gases (e.g., nitric oxide (NO) and carbon monoxide (CO)), among others.
Target cells 106 are cells capable of responding to a specific signal substance produced by a signaling cell. The ability to respond may depend on the cell and on the signal substance. For example, the signal substance thyroxine from the thyroid gland may evoke a response in nearly all cells, whereas the signal substance progesterone from the ovary may evoke a response only in specific cells in the lining of the uterus. The target response may include kinase activity, GTP binding, and/or cyclic nucleotide production.
The ability of a cell to respond to a given signal substance generally is determined by whether the cell includes a receptor for the signal substance. Here, a receptor is any molecule or supramolecular assembly capable of specifically binding a signal substance and initiating a response in a target cell. Representative receptors include cell-surface receptors 110 located on the surface of the target cell and intracellular receptors 112 located within the cytosol 114 or nucleus 116 of the target cell.
The nature of the response initiated by binding of a signal substance is determined by the intracellular machinery to which the receptor is operatively coupled. For example, binding of the neurotransmitter acetylcholine to identical receptors in heart muscle cells and secretory cells causes muscle relaxation in the heart muscle cells and secretion in the secretory cells, due to differences in the associated intracellular machinery.
The remainder of this section examines (1) the receptor mechanisms that cells use to bind signal substances and to communicate this binding to the cell interior, (2) the intracellular pathways that cells use for regulation, (3) the effects of errors in cell-signaling pathways, and (4) selected shortcomings of current cell-signaling assays.
I.A Receptor MechanismsTarget cells generally have receptors capable of specifically binding specific signal substances, including cell-surface receptors and/or intracellular receptors, as described above. Cell-surface receptors are more common and include (A) G-protein-linked receptors, (B) enzyme-linked receptors, and (C) ion-channel-linked receptors. These receptors typically bind large and/or water-soluble signal substances, such as many peptide hormones. Intracellular receptors are less common and include (A) guanylyl cyclase and (B) ligand-activated gene regulatory proteins. These receptors typically bind small and/or water-insoluble signal substances, such as steroid hormones, thyroid hormones, retinoids, vitamin D, and NO.
In the cAMP pathway, the target protein may be adenylyl cyclase (also known as adenylate cyclase), and the G-protein may be a stimulatory G-protein (Gs) that activates the adenylyl cyclase to make cAMP, or an inhibitory G protein (Gi) that inhibits the adenylyl cyclase to prevent it from making cAMP. The cAMP produced by the adenylyl cyclase activates cAMP-dependent protein kinase (A-kinase), which is a serine/threonine kinase that in turn activates or inhibits other enzymes to effect a physiological response. For example, in connection with glycogen metabolism, A-kinase may inhibit glycogen synthase to shut down glycogen synthesis, and simultaneously activate phosphorylase kinase that in turn activates glycogen phosphorylase to break down glycogen. A variety of signal substances use cAMP as a second messenger, including calcitonin, chorionic gonadotropin, corticotropin, epinephrine, follicle-stimulating hormone, glucagon, luteinizing hormone, lipotropin, melanocyte-stimulating hormone, norepinephrine, parathyroid hormone (PTH), thyroid-stimulating hormone, and vasopressin. The level of cAMP may be reduced by phosphodiesterases (PDEs), and the activity of kinases may be reversed by phosphatases, as described below.
In the Ca2+ pathway, the target protein may be a phospholipase with specificity for a phosphoinositide (i.e., inositol phospholipid), and the G-protein may be Gq, which activates the phospholipase to cleave the phosphoinositide to produce an intermediate that releases Ca2+ from the endoplasmic reticulum. For example, the phospholipase phosphoinositide-specific phospholipase C (phospholipase C-β) cleaves the phosphoinositide phosphatidylinositol 4,5-bisphosphate (PIP2) to produce the second messengers inositol triphosphate (IP3) and diacylglycerol. The inositol triphosphate is water soluble and diffuses to the endoplasmic reticulum (ER), where it releases Ca2+ from the ER by binding to IP3-gated Ca2+-release channels in the ER membrane. The diacylglycerol is membrane bound and may be cleaved to form the second messenger arachidonic acid or may activate the Ca2+-dependent serine/threonine kinase protein kinase C that in turn activates or inhibits other enzymes to effect a response. A variety of signal substances use Ca2+ as a second messenger, including acetylcholine, thrombin, and vasopressin.
The signal substance also may bind to intracellular receptors, such as guanylyl cyclase. This enzyme produces cGMP from GTP, which then acts as a second messenger much like cAMP. As described above, cGMP also may be produced by enzyme-linked cell-surface receptors. cGMP is present in most tissues at levels 1/10 to 1/100 those of cAMP. A variety of compounds increase cGMP levels in cells, including (1) the hormones acetylcholine, insulin, and oxytocin, (2) the guanylate cyclase stimulators (and vasodilators) nitroprusside, nitroglycerin, sodium nitrate, and nitric oxide, (3) chemicals such as serotonin and histamine, and (4) peptides such as atrial natriuretic peptide (ANP) that relax smooth muscle.
The level of cyclic nucleotides such as cAMP and cGMP may be controlled by specialized enzymes known as phosphodiesterases (PDEs). These enzymes catalyze the hydrolysis of the 3′-ester bond of cAMP and/or cGMP to form the corresponding noncyclized nucleotide monophosphates AMP and GMP, respectively. More than 30 human PDEs are known, and there is great interest in the pharmaceutical industry in finding inhibitors for PDEs for a broad range of potential therapeutic applications. A selective inhibitor of PDE-5 has been commercialized as the drug Viagra™ (i.e., Sildenafil) for the treatment of male erectile dysfunction. Moreover, several PDE-4 inhibitors are in clinical trials as anti-inflammatory drugs for the treatment of diseases such as asthma
I.B Intracellular Signaling PathwaysTarget cells may have intracellular signaling pathways capable of specifically binding signal substances, including cell-surface receptors and intracellular receptors, as described above. These pathways may include (1) a phosphorylation pathway involving ATP/ADP, and (2) a GTP-binding pathway involving GTP/GDP.
Specialized enzymes control phosphorylation in cells. In particular, protein kinase enzymes transfer phosphate groups to proteins, and protein phosphatase enzymes remove phosphate groups from proteins. Protein kinases and protein phosphatases are found in great variety in eucaryotic cells: a single cell may contain more than 100 different kinases, and one percent of genes may code for kinases.
There are two major categories of protein kinases: (1) serine/threonine (S/T) kinases, and (2) tyrosine kinases. The S/T kinases function by selectively phosphorylating serine and threonine side chains on substrate proteins or peptides. These kinases include cyclic AMP-dependent kinase (A-kinase), cyclic GMP-dependent kinase (G-kinase), protein kinase C(C-kinase), Ca2+-calmodulin-dependent kinase (CaM-kinase), phosphorylase kinase, MAP kinase, and TGF-β receptor, among others. The S/T kinases are predominantly cytosolic. The tyrosine kinases function by selectively phosphorylating tyrosine side chains on substrate proteins or peptides. These kinases include the receptor kinases for epidermal growth factor (EGF), platelet-derived growth factor (PDGF), fibroblast growth factors (FGFs), hepatocyte growth factor (HGF), insulin, insulin-like growth factor-1 (IGF-1), nerve growth factor (NGF), vascular endothelial growth factor (VEGF), and macrophage colony stimulating factor (M-CSF). These kinases also include the nonreceptor kinases associated with the tyrosine-kinase-associated receptors, such as the Src family (Src, Yes, Fgr, Fyn, Lck, Lyn, Hck, and Blk) and Janus family (JAK1, JAK2, and Tyk2) kinases. The tyrosine kinases are predominantly membrane bound. A few kinases function by selectively phosphorylating threonine and tyrosine side chains on substrate proteins or peptides. These kinases include the mitogen-activated protein (MAP) kinase-kinase.
A physiological response may require stimulation by only a single type of signal substance, or may require stimulation by two or more types of signal substances. The latter mechanism permits finer tuning of the physiological response through signal integration. For example, a protein may be activated only by phosphorylation by two different kinases, themselves activated by binding of two different signal substances to two different receptors. Alternatively, a protein may be activated only by concurrent phosphorylation and GTP binding, or by binding of two subunits whose binding is contingent on phosphorylation by separately activated kinases.
I.C Effects of ErrorsErrors in the signal transduction and regulation pathways described above can cause cancer and other diseases. Indeed, a primary cause of cancer is a mutation that makes a stimulatory gene product hyperactive, converting a proto-oncogene into an oncogene. The primary classes of known proto-oncogenes include the following cell-signaling proteins: (1) growth-factor receptors acting via tyrosine kinases, (2) GTP binding proteins, (3) membrane/cytoskeleton-associated tyrosine kinases, (4) cytoplasmic tyrosine kinases, (5) steroid-type growth-factor receptors, and (6) S/T kinases. Consequently, cell-signaling proteins have become important subjects of research and drug development.
II. OVERVIEWBinding targets A and A* generally comprise any two species related by a modification (denoted by the presence or absence of *). A and A* may include molecules and assemblies of molecules such as polypeptides and/or nucleotides, among others. The modification may include phosphate modifications such as phosphorylation, dephosphorylation, cyclization, and/or decyclization, among others, and nonphosphate modifications such as nonphosphate posttranslational modifications of polypeptides, among others. A and A* may be related as substrate and product in a reaction, such as an enzyme-catalyzed reaction. Thus, depending on the direction of the reaction, A and A* in a phosphate modification may be a phosphorylated polypeptide, a nonphosphorylated polypeptide, a cyclized nucleotide, or a noncyclized nucleotide, among others. In some embodiments, A and/or A* may include components intended to facilitate detection of binding between A or A* and BP, such as a luminophore, a quencher, an energy transfer partner, and the like.
BP generally comprises any binding partner capable of binding specifically to binding target A or A* (i.e., the modified species or the unmodified species) but not to both. BP may include any binding partner having the specified binding properties. In some examples, BP may not include a polypeptide and/or an immunoglobulin, and/or a functional portion or fragment thereof.
BP may include a metal. In some examples, the metal may be a metal ion. The metal may be configured to form a metal-ligand coordination complex with the binding target, in which one or more electrons are shared between the metal and the binding target. For example, the metal may be a Lewis acid and particularly a strong Lewis acid. In exemplary embodiments, the metal is a Lewis acid that shares electrons with a Lewis base, such as a phosphate. Accordingly, binding between BP and the binding target may be a covalent interaction and/or may be a charge-charge interaction, among others. Exemplary metals that may be suitable as part (or all) of the binding partner include aluminum, chromium, europium, gallium, iridium, iron, manganese, osmium, platinum, rhenium, ruthenium, scandium, strontium, terbium, titanium, vanadium, yttrium, and/or zirconium, among others. In some examples, the metals may be aluminum, gallium, and/or iron. Alternatively, or in addition, the metals may be strontium, europium, terbium, and/or zirconium.
The metal may be in ionic form. Accordingly, BP may be, include, and/or be formed from a metal salt. BP may include one or more metal ions, including dicationic, tricationic, tetracationic, and/or other polycationic metal ions, among others. Suitable dicationic metal ions may include europium (Eu2+), iridium (Ir2+), osmium (Os2+), platinum (Pt2+), rhenium (Re2+), ruthenium (Ru2+), and/or strontium (Sr2+), among others. Suitable tricationic metal ions may include aluminum (Al3+), chromium (Cr3+), europium (Eu3+), gallium (Ga3+), iron (Fe3+), manganese (Mn3+), scandium (Sc3+), terbium (Tb+3), titanium (Ti3+), vanadium (V3+), and/or yttrium (Y3+), among others. Suitable tetracationic metal ions may include zirconium (Zr4+).
A metal or metal ion may be a binding species of BP required for interaction with the modification on binding target A or A*, for example, the phosphate group on a phosphorylated protein or a noncyclized nucleotide. BP may bind to a substrate such as A or A* only if it is phosphorylated, where the binding between the substrate and the binding partner is substantially nonspecific with respect to the structure of the substrate aside from any phosphate groups. Thus, the binding may occur substantially without regard to the target amino acid or surrounding amino acid sequence in a phosphorylation/dephosphorylation assay, or the base or nucleoside in a cyclization/decyclization assay.
In some examples, BP may include a distinct structure to which the metal is connected or otherwise associated. The distinct structure may be a macromolecule (a protein, nucleic acid, polysaccharide, polymer, and/or the like) and/or an associated solid support (or solid phase). The solid support may be a particle, a membrane, and/or a sample holder (such as a microplate), among others. Here, particles include nanoparticles and microparticles, among others, where nanoparticles are particles with at least one dimension less than about 100 nm, and microparticles are particles with dimensions between about 100 nm and about 10 μm. The metal may be associated with (or “tethered”) to the macromolecule and/or solid support using any suitable linkage mechanism, including hydrogen bonding, ionic bonding, electrostatic binding, hydrophobic interactions, Van der Waals interactions, and/or covalent attachment, among others. Alternatively, or in addition, the metal may be “untethered,” that is, not connected to a distinct structure substantially lacking the metal. However, an untethered metal may be included in a metal-based complex, particularly a macromolecular complex, such as an aggregate, microcrystal, and/or a metal hydroxide/oxide, among others. In some embodiments, the complex may form from a metal (or metal salt) when the metal (or metal salt) is placed in a suitable aqueous environment. The suitable aqueous environment may include buffer components (including pH-modifying components such as an acid or base). In some embodiments, BP may include components intended to facilitate detection of binding between BP and A or A*, such as a luminophore, a quencher, an energy transfer partner, and the like.
Metal salts forming a metal-based complex included in the binding partner may have any suitable formula weight. As used herein, the formula weight is the mass of the smallest repeating unit of the metal salt. The formula weight generally is defined with the metal salt in an anhydrous or hydrated crystalline form, that is, before placed in a liquid. With this definition, water-mediated reactions that produce bridged compounds, hydroxides/oxides, aggregates, etc. are ignored in calculating the formula weight. For example, the metal salt gallium chloride may form larger complexes in certain aqueous environments (see, e.g., Example 23 of Section IV), but has a formula weight (anhydrous) of about 176 grams/mole. In some embodiments, the metal salt may have a formula weight of less than about 105, 104, 103, or 400 grams/mole. Alternatively, or in addition, the metal salt may have a formula weight that is less than the formula or molecular weight of the binding target (for example, the substrate or product), or less than about ten-fold or one-hundred fold the formula or molecular weight of the binding target.
EAA* and EA*A generally comprise any enzymes or other catalysts capable of facilitating reactions converting A to A* and A* to A, respectively. EAA* and EA*A may include, among others, enzymes such as kinases and phosphatases, which catalyze the addition and removal of phosphate groups to and from polypeptides, respectively. EAA* and EA*A also may include enzymes such as cyclases and phosphodiesterases, which catalyze the cyclization and decyclization of nucleotides, respectively, to produce cyclized and noncyclized nucleotides.
MEA* and MEA*A generally comprise any modulators or other agents capable of modulating or otherwise affecting the activity of EAA* and EA*A, respectively. The modulator may be a change in environmental condition, such as a change in sample temperature, but more typically is an enzyme or other reagent added to the sample. The modulator may be a chemical reagent, such as an acid, base, metal ion, organic solvent, and/or other substance intended to effect a chemical change in the sample. Alternatively, or in addition, the modulator may have or be suspected to have a biological activity or type of interaction with a given biomolecule, such as an enzyme, drug, oligonucleotide, nucleic acid polymer, peptide, protein, and/or other biologically active molecule. The modulator may include an agonist or inhibitor capable of promoting or inhibiting, respectively, the activity of the modulated enzyme. For example, in a cyclic nucleotide assay, preferred agonists include magnesium and calmodulin, and preferred inhibitors include isobutylmethylxanthine and Zaprinast.
III. ASSAYSThe step of contacting assay components such as enzymes, enzyme modulators, substrates, products, and/or binding partners with one another and/or with other species generally comprises any method for bringing any specified combination of these components into functional and/or reactive contact. A preferred method is by mixing and/or forming the materials in solution, although other methods, such as attaching one or more components such as the binding partner to a bead or surface, also may be used, as long as the components retain at least some function, specificity, and/or binding affinity following such attachment. Exemplary apparatus having fluidics capability suitable for contacting or otherwise preparing assay components are described in the following U.S. patent applications, which are incorporated herein by reference: Ser. No. 09/777,343, filed Feb. 5, 2001; and Ser. No. 10/061,416, filed Feb. 1, 2002.
One or more of the assay components may comprise a sample, which typically takes the form of a solution containing one or more biomolecules that are biological and/or synthetic in origin. The sample may be a biological sample that is prepared from a blood sample, a urine sample, a swipe, or a smear, among others. Alternatively, the sample may be an environmental sample that is prepared from an air sample, a water sample, or a soil sample, among others. The sample typically is aqueous but may contain biologically compatible organic solvents, buffering agents, inorganic salts, and/or other components known in the art for assay solutions.
The assay components and/or sample may be supported for contact and/or analysis by any substrate or material capable of providing such support. Suitable substrates may include microplates, PCR plates, biochips, and hybridization chambers, among others, where features such as microplate wells and microarray (i.e., biochip) sites may comprise assay sites. Suitable microplates are described in the following U.S. patent applications, which are incorporated herein by reference: Ser. No. 08/840,553, filed Apr. 14, 1997, now abandoned; and Ser. No. 09/478,819, filed Jan. 5, 2000, now U.S. Pat. No. 6,488,892. These microplates may include 96, 384, 1536, or other numbers of wells. These microplates also may include wells having small 50 μL) volumes, elevated bottoms, and/or frusto-conical shapes capable of matching a sensed volume. Suitable PCR plates may include the same (or a similar) footprint, well spacing, and well shape as the preferred microplates, while possessing stiffness adequate for automated handling and thermal stability adequate for PCR. Suitable microarrays include nucleic acid and polypeptide microarrays, which are described in Bob Sinclair, Everything's Great When It Sits on a Chip: A Bright Future for DNA Arrays, 13 T
The step of detecting a response indicative of the extent of binding generally comprises any method for effectuating such detection, including detecting and/or quantifying a change in, or an occurrence of, a suitable parameter and/or signal. The method may include luminescence and/or nonluminescence methods, and heterogeneous and/or homogeneous methods, among others. The method also may include combining two or more detection methods on a single composition, for example, combining luminescence energy transfer and luminescence polarization, among others. Exemplary systems, including apparatus and methods, for combining two or more detection modes are described in U.S. Pat. No. 6,825,921, issued Nov. 30, 2004, which is incorporated herein by reference.
Luminescence and nonluminescence methods may be distinguished by whether they involve detection of light emitted by a component of the sample. Luminescence assays involve detecting light emitted by a luminescent compound (or luminophore) and using properties of that light to understand properties of the compound and its environment. A typical luminescence assay may involve (1) exposing a sample to a condition capable of inducing luminescence from the sample, and (2) measuring a detectable luminescence response indicative of the extent of binding between the member of interest and a corresponding binding partner. Most luminescence assays are based on photoluminescence, which is luminescence emitted in response to absorption of suitable excitation light. However, luminescence assays also may be based on chemiluminescence, which is luminescence emitted in response to chemical excitation, and electrochemiluminescence, which is luminescence emitted in response to electrochemical energy. Suitable luminescence assays include, among others, (1) luminescence intensity, which involves detection of the intensity of luminescence, (2) luminescence polarization, which involves detection of the polarization of light emitted in response to excitation by polarized light, and/or (3) luminescence energy transfer. Luminescence energy transfer involves detection of energy transfer between a luminescent donor and a suitable acceptor (a donor-acceptor energy transfer pair). Such energy transfer may occur with or without the emission of a photon. Generally the efficiency of the energy transfer is dependent on the distance between the donor and acceptor. Accordingly, the amount of energy transfer detected relates, at least partially, to the proximity of the energy donor and acceptor, and thus may be correlated with conversion of substrate to product in an assay. In particular, the energy donor and acceptor may be placed in (or out of) proximity by the enzyme reaction itself, or by selective association of a substrate or product of the enzyme with an association partner. These and other luminescence assays are described below in Example 14 and materials cited therein. Nonluminescence assays involve using a detectable response other than light emitted by the sample, such as absorption, scattering, and/or radioactivity, among others. These and other nonluminescence assays are described in the following materials, which are incorporated herein by reference: U.S. Pat. No. 6,466,316, issued Oct. 15, 2002; and Joseph R. Lakowicz, Principles of Fluorescence Spectroscopy (2nd ed. 1999).
The detectable luminescence response generally comprises a change in, or an occurrence of, a luminescence signal that is detectable by direct visual observation and/or by suitable instrumentation. Typically, the detectable response is a change in a property of the luminescence, such as a change in the intensity, polarization, energy transfer, lifetime, and/or excitation or emission wavelength distribution of the luminescence. For example, energy transfer may be measured as a decrease in donor luminescence, an increase (often from zero) in acceptor luminescence, and/or a decrease in donor luminescence lifetime, among others. The detectable response may be simply detected, or it may be quantified. A response that is simply detected generally comprises a response whose existence merely is confirmed, whereas a response that is quantified generally comprises a response having a quantifiable (e.g., numerically reportable) value such as an intensity, polarization, and/or other property. In luminescence assays, the detectable response may be generated directly using a luminophore associated with an assay component actually involved in binding such as A* or BP, or indirectly using a luminophore associated with another (e.g., reporter or indicator) component. Suitable methods and luminophores for luminescently labeling assay components are described in the following materials, which are incorporated herein by reference: Richard P. Haugland, Handbook of Fluorescent Probes and Research Chemicals (6th ed. 1996); U.S. patent application Ser. No. 09/813,107, filed Mar. 19, 2001; and U.S. patent application Ser. No. 09/815,932, filed Mar. 23, 2001.
Heterogeneous and homogeneous methods may be distinguished by whether they involve sample separation before detection. Heterogeneous methods generally require bulk separation of bound and unbound species. This separation may be accomplished, for example, by washing away any unbound species following capture of the bound species on a solid phase, such as a bead or microplate surface labeled with a tricationic metal ion or other suitable binding partner. The extent of binding then can be determined directly by measuring the amount of captured bound species and/or indirectly by measuring the amount of uncaptured unbound species (if the total amount is known). Homogeneous methods, in contrast, generally do not require bulk separation but instead require a detectable response such as a luminescence response that is affected in some way by binding or unbinding of bound and unbound species without separating the bound and unbound species. Alternatively, or in addition, enzyme activity may result in increased or decreased energy transfer between a donor and acceptor of an energy transfer pair, based on whether the acceptor quenches or not, and based on whether enzyme activity in the assay results in increased or decreased proximity of the donor and acceptor. Homogeneous assays typically are simpler to perform but more complicated to develop than heterogeneous assays.
III.C CorrelatingThe step of correlating generally comprises any method for correlating the extent of binding with the extent of modification of the assay component being analyzed, and/or with the presence and/or activity of an enzyme that affects the modification. The nature of this step depends in part on whether the detectable response is simply detected or whether it is quantified. If the response is simply detected, it typically will be used to evaluate the presence of a component such as a substrate, product, and/or enzyme, or the presence of an activity such as an enzyme or modulator activity. In contrast, if the response is quantified, it typically will be used to evaluate the presence and/or quantity of a component such as a substrate, product, and/or enzyme, or the presence and/or activity of a component such as an enzyme or modulator.
The correlation generally may be performed by comparing the presence and/or magnitude of the response to another response (e.g., derived from a similar measurement of the same sample at a different time and/or another sample at any time) and/or a calibration standard (e.g., derived from a calibration curve, a calculation of an expected response, and/or a luminescent reference material). Thus, for example, in a polarization assay for cyclic nucleotide concentration, the cyclic nucleotide concentration in an unknown sample may be determined by matching the polarization measured for the unknown with the cyclic nucleotide concentration corresponding to that polarization in a calibration curve generated under similar conditions by measuring polarization as a function of cyclic nucleotide concentration. More generally, the following table shows representative qualitative changes in the indicated detectable luminescence response upon binding between A* and BP following a forward reaction A→A*.
This reaction is representative of a phosphorylation reaction performed by a kinase or a decyclization reaction performed by a PDE, assuming that the binding partner binds to the (noncyclized) phosphorylated species. Similarly, the following table shows representative qualitative changes in the indicated detectable luminescence response upon binding of A* and BP following the reverse reaction A* A.
This reaction is representative of a dephosphorylation reaction performed by a phosphatase or a cyclization reaction performed by a cyclase, assuming again that the binding partner binds to the (noncyclized) phosphorylated species.
The following examples describe, without limitation, further aspects of the present teachings. These aspects include (1) the ability of binding partners such as tricationic metal ions to bind specifically to phosphorylated species such as phosphopeptides and nucleotides, and (2) the use of such binding in assays for enzymes and other agents involved in phosphorylation and dephosphorylation (e.g., kinases and phosphatases, respectively) and cyclization and decyclization (e.g., cyclases and PDEs, respectively), among others. The aspects also include (3) experiments comparing metal ions with and without associated beads as phosphate-binding partners in binding assays, (4) experiments testing the effect of other solution components on the ability of a metal salt in the absence of beads to serve as a phosphate-binding partner in binding assays, and (5) experiments testing hypotheses for the action of a metal salt, without associated beads, as a binding partner in the binding assays, and (6) experiments testing the effects of different assay conditions—substrates, buffers, salt concentrations, gallium concentrations, ATP concentrations, etc.—on assay performance. These aspects are applicable to a wide variety of enzymes and enzyme substrates and products.
Example 1This example describes a macromolecular trapping system for use in luminescence polarization and/or energy transfer assays, among others, in accordance with aspects of the present teachings. In this system, Ru2+ is entrapped in small (−20-30 kDa) synthetic polymer macromolecules (MM), which are obtained from PreSens Precision Sensing (Neuburg/Donau, Germany). These macromolecules are relatively hydrophilic, with carboxyl groups on their surfaces for activation. The MM with the entrapped Ru2+ is used as a support to immobilize tricationic metal cations, including Fe3+ and Ga3+. Specifically, the chelator imidodiacetic (IDA) acid is linked to the MM using the secondary amine group of IDA and a carboxyl group on the MM. Afterwards, the MM-IDA is incubated with either FeCl3 or GaCI3. The FeCl3 quenches the luminescence of Ru2+, whereas the GaCI3 does not. The macromolecule loaded with Fe3+ or Ga3+ is denoted MM-Fe or MM-Ga, respectively.
The macromolecular trapping system may be used in a variety of kinase, phosphatase, phosphodiesterase, and/or cyclase assays, as described below in Examples 3-5 and 7-9. In an exemplary assay, a kinase enzyme phosphorylates a luminescently labeled kinase substrate, which binds to the metal cations immobilized on the MM. Binding is detected using polarization and/or energy transfer methods, among others, for example, using apparatus and methods as described herein. Binding is detectable using polarization because binding leads to a decrease in substrate mobility and a concomitant increase in the polarization of light emitted by luminophores bound to the substrate. Similarly, binding is detectable using energy transfer because binding leads to a decrease in separation between the luminophores bound to the substrate and the Ru2+ immobilized in the MM, and a concomitant increase in energy transfer from the Ru2+ (donor) to the luminophore (acceptor).
This approach may be extended by various modifications and/or substitutions. For example, in polarization assays, the Ru2+ may be omitted, if desired. In energy transfer assays, the Ru2+ may be replaced by any energy transfer partner, as long as the energy transfer partner supported by the MM is capable of energy transfer to or from a complementary energy transfer partner supported by the species binding to the MM. Exemplary energy transfer partners are described in U.S. patent application Ser. No. 09/815,932, filed Mar. 23, 2001, which is incorporated herein by reference. Also, the Ru2+ or its analog does not need to be encapsulated in the MM. A luminescent species may be attached directly to a suitable Fe3+ or Ga3+ chelate. In a heterogeneous assay, phosphorylated proteins bound via Ga3+ or Fe3+ to microplates, particles, or inner surfaces of microfluidic devices may be detected after a wash by measuring luminescence intensity. Such detection can take place either directly on the surfaces or in the solution phase by adding an elution solution such as a phosphate buffer. With other detection methods, such as the laser-scanning method used in fluorometric microvolume assay technology (FMAT™) technology (PE Biosystems, Foster City, Calif.), the bound phosphoproteins can be detected directly on the beads, without the need for washing or separation. Other labels such as enzymes also may be used in the heterogeneous format.
Potential difficulties with this system include (1) interference from compounds (e.g., ATP, free phosphate, EDTA, and possibly primary/secondary amines) that may compete with or otherwise affect the interaction between the metal and the phosphorylated protein, and (2) difficulty in maintaining a pH that preserves the affinity and selectivity of the binding between the metal and phosphorylated protein.
Example 2This example describes assays for the presence, activity, substrates, and/or products of kinases in accordance with aspects of the present teachings. Similar assays may be used to analyze phosphatases in which the substrates and products of the kinase reaction become the products and substrates of the phosphatase reaction, respectively.
Kinases catalyze the addition of phosphate groups to appropriate substrates, as shown below:
Thus, the presence and/or activity of a kinase may be detected by a decrease in the concentration of a nonphosphorylated (e.g., polypeptide) substrate and/or by an increase in the concentration of a corresponding phosphorylated product, among others. (The presence and/or activity of a phosphatase may be detected similarly by a decrease in the concentration of a phosphorylated substrate and/or an increase in the concentration of a nonphosphorylated product.) The present teachings provide among others kinase assays that involve contacting a sample containing a candidate kinase (and optionally a modulator thereof) with a luminescently labeled nonphosphorylated polypeptide having at least one amino acid subject to phosphorylation (such as a tyrosine, serine, and/or threonine) and a binding partner that binds specifically to the phosphorylated polypeptide but not to the nonphosphorylated polypeptide. These assays further involve detecting a response indicative of the extent of binding between the polypeptide and the binding partner such as luminescence intensity, polarization, and/or energy transfer, and correlating the response with the extent of phosphorylation or nonphosphorylation of the polypeptide, and thus with the activity of the candidate kinase. The binding partner may include a metal ion such as a tricationic metal ion that interacts with the phosphate group on the phosphorylated polypeptide to facilitate the binding reaction. The binding partner also may include a macromolecule, a nanoparticle, a solid phase portion, a quencher, and/or an energy transfer partner complementary to the luminophore on the polypeptide, depending in part on the detection scheme.
Example 3This example describes experiments to characterize binding between MM-Ga and a fluorescein-labeled di-phosphotyrosine 15-amino-acid peptide tracer denoted tyrosine kinase 1 (TK-1) tracer. These experiments show the utility of the MM-Ga system for detection of phosphorylated tyrosine and the presence and/or activity of tyrosine kinases and phosphatases.
This example describes experiments to characterize binding between MM-Ga and the following mono-serine fluorescein-labeled peptide tracer:
This peptide is termed a “Kemptide,” and the fluorescein-labeled peptide is termed a “fluo-Kemptide.” These experiments use cAMP-dependent protein kinase A (PKA, Promega) as the enzyme and fluo-Kemptide (SEQ ID NO:1) as the substrate. These experiments show the utility of the MM-Ga system for detection of phosphorylated serine and the presence and/or activity of serine/threonine kinases and phosphatases.
Examples 3 and 4 describe homogeneous assays in which metal ions (e.g., Ga3+) immobilized on macromolecules system bind selectively to phosphorylated peptides generated in a kinase reaction. These assays may be used to monitor the time course and/or end point of a kinase (and/or phosphatase) reaction using various luminescence methods, including luminescence polarization.
This example describes a heterogeneous kinase assay in accordance with aspects of the present teachings. Here, the feasibility of using metal-coated plates in the development of generic kinase assays is demonstrated with a commercial Ni2+-coated plate (Pierce, Rockford, Ill.), in which the Ni2+ is replaced with Ga3+. Specifically, 200 μL of a 0.5 M EDTA-containing solution is added to each well of a 96-well Ni2+-coated plate, and the plate is incubated at room temperature for 1 hour. The process is repeated two more times to remove at least substantially all of the Ni2+ from the plate. The plate then is washed 3 times with 10 mM Tris buffer (pH 7.4). Next, 200 μL of a 0.1 M GaCl3 solution is added to each well of the plate, and the plate is incubated overnight at room temperature. The plate is washed three times before being used in a kinase assay. This procedure effectively converts the walls of the plate into assay surfaces capable of binding a phosphorylated substrate but not a nonphosphorylated substrate.
These experiments show the viability of a heterogeneous assay format and the specificity of binding to the tricationic versus dicationic metal ion. The heterogeneous assay format offers many of the advantages of the homogenous assays, including its applicability in principle to any kinase regardless of its substrate specificity. This may save assay developers 3 to 6 months of time and effort in making antibodies that recognize specifically a phosphorylated version of an amino acid sequence. The lack of availability of such special antibodies often is the major obstacle in the development of nonradioactive kinase assays. The heterogeneous assay format also allows for simple detection using luminescence intensity, without requiring polarizers or selection of complementary energy transfer pairs.
Example 6This example describes assays for the presence, activity, substrates, and/or products of phosphodiesterases in accordance with aspects of the present teachings. Similar assays may be used to analyze cyclases in which the substrate of the phosphodiesterase reaction becomes the product of the cyclase reaction.
Phosphodiesterases (PDEs) catalyze the decyclization of cyclic nucleotides to the corresponding noncyclized nucleotide monophosphate, as shown below:
Thus, the presence and/or activity of a PDE may be detected by a decrease in the concentration of a cyclic nucleotide (cNMP) substrate and/or by an increase in the concentration of a corresponding noncyclized nucleotide monophosphate (NMP) product. (The presence and/or activity of a cyclase may be detected similarly by a decrease in the concentration of a nucleotide triphosphate substrate and/or by an increase in the concentration of a corresponding cyclic nucleotide.) The present teachings provide among others PDE assays that involve contacting a sample containing a candidate PDE (and optionally a modulator thereof) with a luminescently labeled cyclic nucleotide and a binding partner that binds specifically to the corresponding noncyclized nucleotide monophosphate but not to the cyclic nucleotide. The binding partner may include one or more of the attributes described above, such as a tricationic metal M3+ (e.g., Al3+, Ga3+, and/or Fe3+) capable of binding a noncyclized phosphate group but not a cyclized phosphate group, and optionally an energy transfer partner and/or quencher. PDE activity may be detected by an increase in NMP binding using any technique capable of measuring such an increase, including luminescence polarization, luminescence resonance energy transfer, luminescence intensity, and/or nonluminescence and/or heterogeneous techniques, among others. For example, PDE activity may be detected following NMP binding by (1) an increase in luminescence polarization (assuming that the lifetime and rotational correlation time of the binding partner are selected so that binding of the NMP to the binding partner measurably decreases the rotational correlation time of the NMP), (2) an increase in luminescence resonance energy transfer (assuming that the binding partner is associated with a suitable energy transfer partner), and/or (3) a decrease in luminescence intensity (assuming that the binding partner is associated with a suitable luminescence quencher).
The assays may include (1) contacting a sample containing a candidate PDE (and/or other cell-signaling component) with a luminescently labeled cyclic nucleotide and a binding partner capable of distinguishing between the cyclic nucleotide and the corresponding nucleotide monophosphate, (2) illuminating the sample with light capable of inducing luminescence in the sample, (3) measuring a property of the luminescence transmitted from the sample, and (4) correlating the property with the presence and/or activity of the cyclic nucleotide and/or the corresponding nucleotide monophosphate and hence the presence and/or activity of an associated enzyme.
The present teachings also provide methods for identifying modulators such as agonists and inhibitors of receptors and/or enzymes involved in the production and/or regulation of cell-signaling molecules, such as the hydrolysis of cyclic nucleotides. The methods may include looking for the effects of a modulator by conducting a method for determining the concentration of a cyclic nucleotide and/or the corresponding nucleotide monophosphate in both the presence and absence of the putative modulator. For example, in a polarization assay in which PDE activity leads to an increase in polarization, a decrease in the measured extent of polarization of the emitted light in the presence of the putative modulator identifies the putative modulator as an inhibitor of the receptor or enzyme, and an increase in the measured extent of polarization in the presence of the putative modulator identifies the putative modulator as an agonist of the receptor or enzyme.
Example 7This example describes end-point and time-course assays for PDE 5 in accordance with aspects of the present teachings, showing in part the utility of the MM-Ga system in PDE assays. These assays use the following components, among others: (1) cGMP-specific PDE (type V, Calbiochem, La Jolla, Calif.), (2) N-methylanthraniloyl (MANT) cGMP substrate (Molecular Probes, Eugene, Oreg.), and (3) MM-Ga, as described in Example 1. MANT is a compact blue fluorescing luminophore that attaches to the cGMP via the ribose ring of the cGMP. These assays show the utility of the MM-Ga system for detection of noncyclized GMP and the presence and/or activity of cGMP-specific PDEs.
This example describes alternative PDE assays in accordance with aspects of the present teachings. These assays are presented in a homogenous, nonradioactive format using a carboxyfluorescein labeled cGMP substrate. The assay also may be used in a heterogeneous format and/or with an alternative luminescent cGMP and/or cAMP. These assays further show the utility of the MM-Ga system for detection of noncyclized GMP and the presence and/or activity of cGMP-specific PDEs, including the use of a different luminophore than the MANT of Example 7.
This example describes several aspects of the present teachings, including (1) use of Ga3+-coated nanoparticles as the binding component in the assay, (2) applications to the detection of PDE 4 enzyme, with fluorescein-labeled cAMP as substrate, and (3) applications to the detection of PDE 1 enzyme, with both fluorescein-labeled cAMP and fluorescein-labeled cGMP as substrates.
As discussed in Example 1, synthetic polymer macromolecules (MM) can be substituted with other materials that have a high molecular weight and that tricationic cations (i.e., Fe3+, Ga3+) can be immobilized on. Here, we use selected nanoparticles, including polystyrene nanoparticles having an average diameter of about 40 nm. The nanoparticles are from Bangs Laboratory (Fisher, Ind.), and are modified after acquisition from the vendor to attach Ga3+ on the surfaces of the particles.
This example shows representative tracers for use in cyclic nucleotide assays, particularly luminescence-polarization-based cyclic nucleotide assays. General structures for such tracers are shown below for (A) cAMP and (B) cGMP:
Here, X and R1 represent linkers, which optionally and independently may be present or absent, and Fl represents a reporter species. X may include among others any alkyl, allyl, or aryl linker with ester or ether bonds to the cyclic nucleotide, including —OC(═O)—CH2CH2C(═O)—. R1 may be any linker joining FL to the nucleotide, directly, or indirectly through X, including a rigid linker having (two) reactive groups for coupling, one to FL and one to the nucleotide. For example, R1 may be a diamino-alkyl, -cycloalkyl, -aryl, or -allyl group, or a dihydroxy group that forms an amide or ester, respectively, with the groups X and Fl. Fl may include any suitable reporter species, such as a luminophore for luminescence assays or an isotope for radioassays. For example, Fl may include a fluorescein or rhodamine that forms a thiourea, ester, or amide bond with the group X. Preferred structures include 1,2 and 1,4-diaminocyclohexyl-linked tracers, as described in U.S. patent application Ser. No. 09/768,661, filed Jan. 23, 2001, which is incorporated herein by reference.
Example 11This example describes methods and kits for detecting phosphate modifications and/or associated enzymes and modulators in whole cells. The methods generally comprise growing cells under desired conditions, lysing the cells, incubating the cells before and/or after lysis with one or more reagents, and detecting the presence, quantity, and/or activity of species and/or reactions of interest. The kits generally comprise collections of reagents and/or other materials of interest, including substrates, binding partners, and/or lysis buffers, among others. The methods and kits are described greater detail in the context of cyclic nucleotide assays for adherent and suspended cells in Examples 8 and 9 of U.S. patent application Ser. No. 09/768,661, filed Jan. 23, 2001, which is incorporated herein by reference.
Example 12This example describes miscellaneous applications and other uses for the various assays described herein.
The applications include detecting any of the modifications, enzymes, and/or modulators identified herein and/or in the following U.S. patent applications, which are incorporated herein by reference: Ser. No. 09/768,661, filed Jan. 23, 2001; and Ser. No. 09/596,444, filed Jun. 19, 2000. The modifications include phosphorylation, dephosphorylation, cyclization, and/or decyclization, among others, as described above. The enzymes include kinases, phosphatases, cyclases, and/or phosphodiesterases, among others, including variants such as isoenzymes thereof. For example, the cyclases include adenylyl cyclase and guanylyl cyclase, among others, and the phosphodiesterases include PDE 1 through PDE 10, among others. The modulators include modulators of these enzymes, among others. For example, the cyclase modulators include forskolin and ODQ, among others, and the phosphodiesterase modulators include cilostamide, dipyridamole, EHNA hydrochloride, etazolate hydrochloride, MBCQ, MMPX, MY-5445, Ro 20-1724, rolipram, siguazodan, vinpocetine, and Zaprinast, among others.
The applications also include combining assays for different modifications, enzymes, and/or modulators to form integrated assays, for example, by combining a phosphorylation assay and a cyclization assay to study signaling mechanisms involving multiple cell-signaling pathways.
Example 13This example describes kits for use in performing assays in accordance with aspects of the present teachings. The kits may include substrates and/or binding partners for performing the assays described herein. These substrates and/or binding partners may include luminophores, quenchers, and/or energy transfer partners, among others. The kits also may include sample holders such as microplates or biochips that have been treated to act as binding partners. The kits optionally may include additional reagents, including but not limited to buffering agents, luminescence calibration standards, enzymes, enzyme substrates, nucleic acid stains, labeled antibodies, or other additional luminescence detection reagents. The substrates, binding partners, and/or additional reagents optionally are present in pure form, as concentrated stock solutions, or in prediluted solutions ready for use in the appropriate energy transfer assay. Typically, the kit is designed for use in an automated and/or high-throughput assay, and so is designed to be fully compatible with microplate readers, microfluidic methods, and/or other automated high-throughput methods.
The kit may include various reagents for performing binding assays. The reagents may include a binding reagent and/or one or more binding buffers. The binding reagent may include particles (such as beads). Alternatively, or in addition, the binding reagent may include one or more metals (or metal ions), particularly a strong Lewis acid and/or a metal salt. Exemplary metals or metal ions that may be included are described elsewhere in the present teachings. The binding reagent may be provided in a liquid, particularly an aqueous liquid of any suitable pH. In some examples, the binding reagent may be supplied in an aqueous buffer and/or in an acidic or basic aqueous liquid. The acidic liquid may include any suitable acid, such as hydrochloric acid. The binding reagent may be provided as a concentrated stock configured to be diluted to a lower concentration, for example, in a binding buffer, for use in binding assays. The binding buffer(s) may be provided to be used directly or supplied as a concentrated stock to be diluted any suitable amount. In some embodiments, the binding buffer may be a set of two or more distinct binding buffers configured to be used individually or combined at one or more different ratios according to an aspect of the binding assay to be performed. In some examples, a ratio of the binding buffers (or diluted versions thereof), or one of a set of binding buffers, may be selected (and the binding buffers mixed), according to an aspect of the binding target used in the binding assay. The aspect of the binding target may be its size, structure, charge (predicted, known, or measured, among others), sequence, molecular weight, and/or the like.
Each binding buffer may include any suitable composition. For example, a binding buffer may include a buffering agent (such as acetate, propionate, morpholinoethane-sulfonic acid (MES), etc.), a salt (such as sodium chloride, potassium chloride, etc.), a detergent(s) (ionic or nonionic), and/or the like. In some examples, the kit may include two or more binding buffers of different composition. The two or more binding buffers may be different in pH, buffering agent, buffer concentration, salt identity or concentration, etc. Such binding buffers may be used separately in different binding assays (e.g., selected according to an aspect of the binding reaction and/or binding target) or may be used in combination.
Example 14This example describes exemplary luminescence assays. Further aspects of these assays as well as additional luminescence assays and apparatus for performing luminescence assays are described in the following materials, which are incorporated herein by reference: U.S. Pat. No. 6,097,025, issued Sep. 24, 1998; U.S. patent application Ser. No. 09/349,733, filed Jul. 8, 1999; U.S. Provisional Patent Application Ser. No. 60/267,639, filed Feb. 10, 2001; and Joseph R. Lakowicz, Principles of Fluorescence Spectroscopy (2nd ed. 1999).
Luminescence, as defined above, is the emission of light from excited electronic states of atoms or molecules, including photoluminescence, chemiluminescence, and electrochemiluminescence, among others. Luminescence may be used in a variety of assays, including (A) intensity assays, (B) polarization assays, and (C) energy transfer assays, among others.
A. Intensity AssaysLuminescence intensity assays involve monitoring the intensity (or amount) of light emitted from a composition. The intensity of emitted light will depend on the extinction coefficient, quantum yield, and number of luminescent analytes in the composition, among others. These quantities, in turn, will depend on the environment of the analyte, among others, including the proximity and efficacy of quenchers and energy transfer partners. Thus, luminescence intensity assays may be used to study binding reactions, among other applications.
B. Polarization AssaysLuminescence polarization assays involve the absorption and emission of polarized light. Here, polarization refers to the direction of the light's electric field, which generally is perpendicular to the direction of the light's propagation. In a luminescence polarization assay, specific molecules within a composition are labeled with one or more luminophores. The composition then is illuminated with polarized excitation light, which preferentially excites luminophores having absorption dipoles aligned parallel to the polarization of the excitation light. These molecules subsequently decay by preferentially emitting light polarized parallel to their emission dipoles. The extent of polarization of the total emitted light depends on the extent of molecular reorientation during the time interval between luminescence excitation and emission, which is termed the luminescence lifetime, τ. In turn, the extent of molecular reorientation depends on the luminescence lifetime and the size, shape, and environment of the reorienting molecule. Thus, luminescence polarization assays may be used to quantify binding reactions and enzymatic activity, among other applications. In particular, molecules commonly rotate (or “tumble”) via diffusion, with a rotational correlation time τrot that is proportional to their volume, or the cube of their radius of gyration. (This cubic dependence on radius makes polarization assays very sensitive to binding.) Thus, during their luminescence lifetime, relatively large molecules will not reorient significantly, so that their total luminescence will be relatively polarized. In contrast, during the same time interval, relatively small molecules will reorient significantly, so that their total luminescence will be relatively unpolarized.
The relationship between polarization and intensity is expressed by the following equation:
Here, P is the polarization, l∥ is the intensity of luminescence polarized parallel to the polarization of the excitation light, and l⊥ is the intensity of luminescence polarized perpendicular to the polarization of the excitation light. P generally varies from zero to one-half for randomly oriented molecules (and zero and one for aligned molecules). If there is little rotation between excitation and emission, l∥ will be relatively large, l⊥ will be relatively small, and P will be close to one-half. (P may be less than one-half even if there is no rotation; for example, P will be less than one-half if the absorption and emission dipoles are not parallel.) In contrast, if there is significant rotation between absorption and emission, l∥ will be comparable to l⊥, and P will be close to zero. Polarization often is reported in milli-P units (1000×P), which for randomly oriented molecules will range between 0 and 500, because P will range between zero and one-half.
Polarization also may be described using other equivalent quantities, such as anisotropy. The relationship between anisotropy and intensity is expressed by the
Here, r is the anisotropy. Polarization and anisotropy include the same information, although anisotropy may be more simply expressed for systems containing more than one luminophore. In the description and claims that follow, these terms may be used interchangeably, and a generic reference to one should be understood to imply a generic reference to the other.
The relationship between polarization and rotation is expressed by the Perrin equation:
Here, P0 is the polarization in the absence of molecular motion (intrinsic polarization), τ is the luminescence lifetime (inverse decay rate) as described above, and τrot is the rotational correlation time (inverse rotational rate) as described above.
The Perrin equation shows that luminescence polarization assays are most sensitive when the luminescence lifetime and the rotational correlation time are similar. Rotational correlation time is proportional to molecular weight, increasing by about 1 nanosecond for each 2,400 dalton increase in molecular weight (for a spherical molecule). For shorter lifetime luminophores, such as fluorescein, which has a luminescence lifetime of roughly 4 nanoseconds, luminescence polarization assays are most sensitive for molecular weights less than about 40,000 daltons. For longer lifetime probes, such as Ru(bpy)2dcbpy (ruthenium 2,2′-dibipyridyl 4,4′-dicarboxyl-2,2′-bipyridine), which has a lifetime of roughly 400 nanoseconds, luminescence polarization assays are most sensitive for molecular weights between about 70,000 daltons and 4,000,000 daltons.
Luminescence polarization assays may be used in a variety of formats. In one format, the concentration of an analyte in solution can be measured by supplying a labeled tracer that competes with the analyte for a binding moiety, particularly a binding moiety larger than the labeled tracer. In this “competitive” format, the concentration of the analyte is inversely correlated with the enhancement of luminescence polarization in the light emitted by the tracer when it competitively binds the common moiety. In another format, the concentration of a target can be measured by supplying a labeled tracer that is capable of binding the target. In this case, the enhancement of polarization is a direct measure of the concentration of target. The target further may be, for example, an activated receptor, where activation can be indirectly measured by the directly measured concentration of a generated molecule or by its binding to labeled tracer per se.
C. Energy Transfer AssaysEnergy transfer is the transfer of luminescence energy from a donor luminophore to an acceptor without emission by the donor. In energy transfer assays, a donor luminophore is excited from a ground state into an excited state by absorption of a photon. If the donor luminophore is sufficiently close to an acceptor, excited-state energy may be transferred from the donor to the acceptor, causing donor luminescence to decrease and acceptor luminescence to increase (if the acceptor is luminescent). The transfer may occur if the absorption spectrum of the acceptor overlaps the emission spectrum of the donor (matching electron vibrational levels). The efficiency of this transfer is very sensitive to the separation R between donor and acceptor, decaying as 1/R−6. Thus, the separation between donor and acceptor in energy transfer assays may be very small, typically about 1-10 μm. The efficiency of this transfer also is sensitive to the relative orientations of the donor and acceptor; for example, the donor and acceptor transition dipole orientations should be approximately parallel for optimal energy transfer. Energy transfer assays use energy transfer to monitor the proximity of donor and acceptor, which in turn may be used to monitor the presence or activity of an analyte, among others.
Energy transfer assays may focus on an increase in energy transfer as donor and acceptor are brought into proximity. These assays may be used to monitor binding, as between two molecules X and Y to form a complex X:Y. Here, colon (:) represents a noncovalent interaction. In these assays, one molecule is labeled with a donor D, and the other molecule is labeled with an acceptor A, such that the interaction between X and Y is not altered appreciably. Independently, D and A may be covalently attached to X and Y, or covalently attached to binding partners of X and Y.
Energy transfer assays also may focus on a decrease in energy transfer as donor and acceptor are separated. These assays may be used to monitor cleavage, as by hydrolytic digestion of doubly labeled substrates (peptides, nucleic acids). In one application, two portions of a polypeptide are labeled with D and A, so that cleavage of the polypeptide by a protease such as an endopeptidase will separate D and A and thereby reduce energy transfer. In another application, two portions of a nucleic acid are labeled with D and A, so that cleave by a nuclease such as a restriction enzyme will separate D and A and thereby reduce energy transfer.
Energy transfer between D and A may be monitored in various ways. For example, energy transfer may be monitored by observing an energy-transfer induced decrease in the emission intensity of D and increase in the emission intensity of A (if A is a luminophore). Energy transfer also may be monitored by observing an energy-transfer induced decrease in the lifetime of D and increase in the apparent lifetime of A.
In a preferred mode, a long-lifetime luminophore is used as a donor, and a short-lifetime luminophore is used as an acceptor. Suitable long-lifetime luminophores include metal-ligand complexes containing ruthenium, osmium, etc., and lanthanide chelates containing europium, terbium, etc. In time-gated assays, the donor is excited using a flash of light having a wavelength near the excitation maximum of D. Next, there is a brief wait, so that electronic transients and/or short-lifetime background luminescence can decay. Finally, donor and/or acceptor luminescence intensity is detected and integrated. In frequency-domain assays, the donor is excited using time-modulated light, and the phase and/or modulation of the donor and/or acceptor emission is monitored relative to the phase and/or modulation of the excitation light. In both assays, donor luminescence is reduced if there is energy transfer, and acceptor luminescence is observed only if there is energy transfer.
Example 15This example shows assays with improved signals, signal-to-noise ratios, and/or signal-to-background ratios.
Signal may be enhanced in several ways, including (1) using a high color temperature light source, such as a xenon arc lamp, in a continuous illumination mode, (2) using a dichroic or multi-dichroic beamsplitter, and/or (3) using a sample holder whose shape is “matched” to the shape of the optical beam of the instrument, especially if the sample holder is elevated to bring the sample closer to a detector. The high color temperature light source increases the number of usable photons, which is important because the lower limit of the signal-to-noise ratio is set by the square root of the total number of photons collected in the measurement. These enhancements are described in more detail in the following U.S. patent applications, which are incorporated herein by reference: Ser. No. 09/349,733, filed Jul. 8, 1999; Ser. No. 09/478,819, filed Jan. 5, 2000; and Ser. No. 09/494,407, filed Jan. 28, 2000.
Signal-to-noise ratios can be enhanced at least in part by increasing signals, for example, by using the techniques described in the previous paragraph.
Signal-to-background ratios can be enhanced in several ways, including (1) using confocal optical systems having a sensed volume to avoid luminescence from the microplate walls, (2) selecting a microplate or other substrate that increases the signal and reduces the luminescent background from materials in the microplate, (3) selecting the light sources, luminescence filters, optics, signal collection electronics, and mechanical system used in the luminescence detection optical system for maximum signal-to-background ratio, and (4) utilizing signal processing, background subtraction, and luminescence lifetime techniques, particularly FLAMe™ methodology for background reduction, as described below. These enhancements are described in more detail in the following U.S. patent and U.S. patent applications, which are incorporated herein by reference: U.S. Pat. No. 6,071,748, issued Apr. 17, 1998; Ser. No. 09/349,733, filed Jul. 8, 1999; Ser. No. 09/478,819, filed Jan. 5, 2000; and Ser. No. 09/494,407, filed Jan. 28, 2000.
Example 16This example shows mechanisms for increasing the change in polarization that accompanies a change in binding, so that the change in binding can be measured more easily. These mechanisms may be used in any of the assays described here involving luminescently labeled species, such as labeled cyclic nucleotides and labeled nonhydrolyzable GTP analogs, among others.
The change in polarization upon binding can be increased by making any linker between the luminophore and the labeled species (e.g., the cyclic nucleotide or GTP analog) as short and/or rigid as possible, while maintaining relevant substrate properties for the enzymes involved in the assay. Short and/or rigid linkers will restrict luminophore motion relative to the labeled species, reducing the “propeller effect” so that the luminophore more accurately reports the motion of both the free and bound labeled species. The rigidity of the linker may be increased by avoiding using hexanoic acid linkers, which typically are long and flexible, and by using cyclic linkers and amide groups in place of methylene groups, among other mechanisms.
The change in polarization upon binding also can be increased by including an appropriately positioned energy transfer acceptor on the binding partner, so that energy transfer will occur from the luminophore to the acceptor upon incorporation. Such energy transfer will shorten the lifetime of the luminophore, thereby increasing its polarization (because polarization varies inversely with lifetime, all else being equal).
The change in polarization upon binding also can be increased by decreasing the mobility of the binding partner for the labeled species. Mobility can be decreased by increasing the size of the binding partner, either directly or by forming a complex with a mass label. Suitable mass labels include other molecules and beads, among others. The use of mass labels is described in detail in U.S. patent application Ser. No. 09/768,742, filed Jan. 23, 2001, which is incorporated herein by reference. Mobility also can be decreased by attaching the binding partner to a surface, such as the surface of a sample holder. Attachment to other molecules, beads, and/or surfaces may be accomplished using any of a number of well-known reactive groups.
The assays provided by the present teachings may have advantages over prior assays for detecting molecular modifications. The existence and/or identity of these advantages will depend on such as (but not always requiring) one or more of the following. First, they may be used without radioactivity. Second, they may be homogenous, so that they do not require physical separation steps or wash steps. Third, they may have stable endpoints, so that results are relatively insensitive to the timing of any measurement or detection steps. Fourth, they may be sensitive, so that picomolar amounts of cyclic nucleotides may be detected. Fifth, they may be used with solution and cell-based samples.
Example 17This example lists exemplary peptides and phosphopeptides that may be used in molecular modification assays according to the present teachings, and exemplary uses for these peptides and phosphopeptides; see Tables 5 and 6. A number of these peptides/phosphopeptides are used in subsequent Examples for exemplary binding assays.
This example describes an experiment to test if a metal salt, gallium chloride, in the absence of associated beads, can serve as a phosphate-binding partner to distinguish between phosphorylated and nonphosphorylated forms of a peptide in a binding assay; see
The binding assay was carried out by preparing a set of binding reactions having different ratios of fluorescein-labeled (FL) Crosstide and Phosphocrosstide peptides (SEQ ID NOS:6 and 7, respectively). The total peptide concentration before dilution with binding partner was 100 nM in binding buffer (20 μL/well). Each binding reaction included gallium chloride as the binding partner. Gallium chloride was prepared as a concentrated solution at 300 mM in 0.1M HCl. The concentrated solution was diluted to 300 μM in binding buffer, and 60 μL was added to each binding reaction. Fluorescence polarization (mP) was measured. (Binding buffers are described in more detail below in Example 22.)
This example describes experiments comparing peptide binding in binding assays that include a gallium salt without beads or associated with beads; see
Each binding reaction initially included fluorescein labeled (“FL”) Crosstide or Phosphocrosstide (SEQ ID NOS:6 and 7, respectively) at 100 nM in 20 μL of binding buffer. Solutions of gallium chloride were prepared as described in Example 18, with or without associated beads. The gallium chloride solutions were diluted 1:400 in binding buffer, and 60 μL were added to the binding reactions. A subset of the binding reactions also included 100 μM ATP. Fluorescence polarization was measured.
This example describes experiments to test the ability of an untethered metal salt (“no beads”) to act as a phosphate-specific binding partner in a kinase assay; see
Kinase reactions with MAPKAP Kinase 2 enzyme were performed in 20 μL using 100 nM substrate (SEQ ID NO:11) and 10 μM ATP, as described generally in Appendices A and B of U.S. Provisional Patent Application Ser. No. 60/436,725, filed Dec. 26, 2002, which is incorporated herein by reference. Duplicate sets of reactions were prepared with a range of amounts of MAPKAP Kinase 2 enzyme and were reacted for about 60 minutes and then stopped with acid (low pH solution). After the reaction was stopped, the reaction products of each set were incubated with a binding solution (60 μL) for 30 minutes at room temperature. The binding solutions for one set included gallium ions associated with beads (“beads”) and for the other set included 300 μM gallium chloride without beads (“no beads”). After incubation with the binding solutions, fluorescence polarization was measured.
This example explores possible hypotheses for the ability of a metal salt, in the absence of beads, to affect fluorescence polarization substantially, and describes experiments to distinguish between these hypotheses; see
A number of hypotheses may account for the ability of a “beadless” metal salt to produce the observed change in fluorescence polarization as a binding partner in the experiments of Examples 18-20. Two of these hypotheses are considered here in more detail. Hypothesis 1: interaction between the metal salt, binding target (phosphopeptide), and possibly other components in the binding reaction shortens the fluorescence lifetime of the fluorophore, producing a corresponding increase in fluorescence polarization. Hypothesis 2: a macromolecular complex forms from the metal salt in solution in situ, and the binding target binds to that complex. Furthermore, if a macromolecular complex forms in solution, the complex may form, for example, (a) before addition to the binding reaction, or (b) after addition to the binding reaction.
a. Experiments for Hypothesis 1
Hypothesis 1 postulates that interactions between components of the assay shorten the fluorescence lifetime of the fluorophore, thereby increasing fluorescence polarization. This hypothesis was tested by measuring the effect of a beadless metal salt on fluorescence lifetime of a peptide-conjugated fluorescein fluorophore (FL-Crosstide/Phosphocrosstide). The fluorescence lifetimes of FL-Crosstide and FL-Phosphocrosstide were measured on a FLARe™ instrument in a buffer that includes or lacks gallium chloride.
The following table shows the results of these measurements. The fluorescence lifetime of FL-Crosstide (+/−gallium) and FL-Phosphocrosstide (no gallium) was measured as 3.7 nsec. (As described earlier, each of these conditions showed a low FP signal and thus little binding to gallium(III).) By contrast, FL-Phosphocrosstide in the presence of gallium chloride showed a slight increase in fluorescence lifetime, a value of 4.3 nsec. However, this increase in fluorescence lifetime should produce a decrease in fluorescence polarization, an effect opposite to that observed in Examples 18-20. Accordingly, Hypothesis 1 is not supported by these results.
b. Experiments for Hypothesis 2
Hypothesis 2 postulates that a large (macromolecular) complex of the binding partner is produced by gallium chloride in solution. Such a complex would be large enough to produce a substantial change in fluorescence polarization by slowing rotation of the binding target. In addition, such a complex may be large enough to be retained by a size exclusion matrix, such as a dialysis membrane. This hypothesis was tested using a variety of dialysis experiments that test for the presence of a large complex of Ga(III) and the time point in the binding protocol at which such a complex may be produced; see
i. Experiment 1
Gallium chloride in binding buffer was tested as a binding partner, with or without overnight dialysis before the binding assay. A solution of 300 μM gallium chloride was prepared in binding buffer (see Example 18). The solution was divided into two portions and placed at room temperature. The first portion was incubated overnight without dialysis. In contrast, the second portion was dialyzed against the buffer overnight using a membrane with an exclusion limit of 12-14 kDa. This dialysis should remove Ga(III) that is not in a complex large enough to exceed the exclusion limit. After overnight incubation, each binding solution was added to a binding reaction, prepared as described in Example 18, with a binding target of either FL-Crosstide or FL-Phosphocrosstide.
ii. Experiment 2
Additional dialysis experiments were performed using a pre-mixed binding reaction that included either FL-Crosstide or FL-Phosphocrosstide. The ratios and concentrations used were as described for the binding reaction of Example 18 above, but in a larger volume to allow dialysis. Each pre-mixed binding reaction then was aliquoted to provide duplicate portions that were incubated overnight, either with or without dialysis against a binding buffer lacking Crosstide and gallium chloride.
c. Summary
These results, taken together, suggest that the gallium is forming and/or involved in forming a large complex (larger than the exclusion limits of the dialysis membrane) in the binding solution. This complex appears to form before addition of binding target or other components of the binding reaction. Moreover, the complex appears stable, because the complex still was effective in binding assays following dialysis overnight.
Example 22This example describes experiments that identify components of the binding buffer that contribute to the formation of macromolecular, phosphate-binding structures; see
a. Buffer Set 1: Salt, BSA, and pH Effects
Experiments were performed using a first set of binding buffers, based on MES 2-(4-morpholino)-ethane sulfonic acid (MES), which differed in salt concentration, BSA (bovine serum albumen) concentration, and/or pH. These binding buffers had one of the following four buffer, salt, and protein compositions:
1) 50 mM MES;
2) 50 mM MES, 500 mM NaCl;
3) 50 mM MES, 0.1% BSA; or
4) 50 mM MES, 500 mM NaCl, 0.1% BSA.
In addition, for each composition, these binding buffers had one of the following five different pH values: 5.5, 5.0, 4.5, 4.0, and 3.5.
The resulting twenty different binding buffers each were used to dilute a concentrated stock solution of gallium chloride to 300 μM (see Example 18), to produce corresponding binding solutions. In turn, each binding solution was tested in a binding reaction at a 3:1 ratio with binding buffer alone, binding buffer with 100 nM FL-Crosstide, or binding buffer with 100 nM FL-Phosphocrosstide (see Example 18).
b. Buffer Set 2: Detergent and pH Effects
Additional experiments were performed using a second set of binding buffers, based on acetate, which differed in detergent concentration and pH. These binding buffers were prepared with either 50 mM acetate alone or 50 mM acetate with 0.1% Triton X-100, each at five pH values: 5.5, 5.0, 4.5, 4.0, and 3.5.
The resulting ten different binding buffers each were used to dilute a concentration stock solution of gallium chloride to 300 μM (see Example 18), to produce corresponding binding solutions. In turn, each binding solution was tested in a binding reaction at a 3:1 ratio with binding buffer with 100 nM FL-CDK peptide (SEQ ID NO:13), 100 nM FL-Crosstide (SEQ ID NO:6), or 100 nM FL-Phosphocrosstide (SEQ ID NO:7).
c. Conclusions
These experiments show that the ability to form a specific phosphate-binding complex from gallium chloride is independent of buffer identity, ionic strength, the presence or absence of BSA, and the presence or absence of detergent, under the range of conditions tested. However, the extent of nonspecific interaction between gallium chloride and the nonphosphorylated peptide is affected by these buffer components.
Example 23This example describes, without limitation, exemplary models for the structure of Ga(III) in buffer or water. The precise nature or origin of the structure is not important to the efficacy of the assay; however, an understanding of the mechanism may help to improve or optimize assay conditions and results.
In aqueous solution, gallium ions may form amphoteric hydroxides and/or oxo ions that have a solubility minimum around pH 3-5. Due to this solubility minimum, microcrystalline structures or an amorphous complex may be produced at acidic pH. For example, Avivi et al. presents the following equation (J. Am. Soc (1999) 121, 4196-4199):
Ga3+(aq)+2H2O(l)GaO(OH)(s)+3H+(aq)
Accordingly, a solid may be formed as GaO(OH) in binding reactions. Other equations that may explain the formation of a larger complex from gallium ions in solution are as follows:
[Ga(H2O)6]3+[Ga(H2O)5OH]2++H+ 1)
2[Ga(H2O)5OH]2+[(H2O)5GaOGa(H2O)5]4++H2O 2)
Accordingly, bridged networks of gallium hydroxides/oxides may be formed by extending these equations to produce larger structures.
Example 24This example describes additional aspects of the assays.
Suitable or preferred assay conditions for beadless (or untethered binding partner) assays may vary, depending on the enzyme (e.g., kinase versus phosphatase, specific type of kinase, etc.) and/or other reaction component(s). For example, because interactions in the different binding buffers appear to be peptide specific, suitable assays conditions for tyrosine kinases (especially receptor kinases), which preferentially interact with peptide substrates having a high content of carboxy amino acids, may differ from suitable assay conditions for other kinases, which preferentially interact with different peptide substrates. Further aspects of the effect, on binding assays, of the number of carboxy groups in a binding target, and exemplary buffer adjustments to improve binding assays based on the number of carboxy groups, are presented in the Examples below.
Assays may be performed in any suitable format. Thus, in some embodiments, microtiter plates or other solid phases (e.g., a chip or array) could be plated with Ga-oxide or hydroxides to create a phosphate binding surface, which could be used in phosphorylation assays (substrate-, auto-) together with any suitable detection modality, such as quenching. (Quenching can be performed using any suitable energy transfer pairs, for example, using quantum dots and/or suitable pairs disclosed in Richard P. Haugland, Handbook of Fluorescent Probes and Research Chemicals (6th ed. 1996), which is incorporated herein by reference. Suitable no-wash assays can employ a quenched product that unquenches when bound to a (Ga-coated) surface, or a quenching (masking) dye in the supernatant, so that only the product bound to the surface is observable.) Alternatively, or in addition, different metals, such as Ga(III) and Tb(III) or other lanthanides, among others, could be co-precipitated to create a mixed structure that binds phosphate. The mixed structure may be useful, for example, in time-resolved fluorescence resonance energy transfer (TR-FRET).
Example 25This example describes exemplary parameters that may affect the sensitivity and/or reproducibility of molecular modification assays.
The molecular modification assays described herein are intended to work well under a variety of assay conditions. However, despite their great flexibility, these assays may be affected by a variety of specific factors, including (A) instability of the binding solution, (B) the structure/sequence of the substrate, and (C) sensitivity to ATP concentration. Thus, there is a need for assay components and conditions that increase the stability of the binding solution, that increase the number of different substrates that can be used (e.g., by decreasing sequence specificity), and/or that increase ATP tolerance, among others.
1. Instabilities in the Binding Solution
The binding solution (binding buffer mixed with binding reagent (binding partner such as Ga(III))) may lose binding capacity over time (although this loss may not be apparent except with kinase substrates having no or one acidic residues). This instability may be addressed in several ways. First, the binding solution may be mixed fresh, close to the time of use. Second, the binding solution may be added in two steps: (1) add binding buffer to reduce pH, and (2) add binding reagent in water (which may be generally stable). However, this introduces an additional step. Third, binding solution may be kept cool (e.g., on ice), since this slows the loss of binding sites. Fourth, gallium may be added to make up for lost binding capacity. However, above a certain concentration, added gallium may cause precipitation in the binding solution. Thus, these approaches all have shortcomings, especially for high-throughput screening.
2. Sequence Specificities of the Substrate
The choice of enzyme substrate may be limited by various factors, including sequence specificities. For example, substrates with too few acidic residues may be sensitive to losses in buffer binding capacity, as mentioned above, and substrates with too many acidic residues may give high backgrounds. These sequence specificities may be addressed in several ways. First, substrates with good (low) Km's may be selected, for example, by choosing substrates with few acidic residues and/or by changing acidic residues to nonacidic residues (e.g., by amidation). This approach may be especially useful with known substrates (such as an alpha-tubulin derived peptide as a substrate for Zap-70 and Syk). Second, assay results simply may be accepted with higher backgrounds (e.g., for a Blk/Lyn substrate peptide). However, neither approach is ideal.
3. Sensitivities to ATP Concentration
The choice of enzymes may be limited by the number of binding sites, due to sensitivities to ATP concentrations. These limitations are greater for enzymes with high Km's for ATP (such as CDK). These limitations may be addressed, for example, by increasing ATP concentration as high as possible without losing signal, especially for enzymes with high Km's.
Example 26This example describes experiments testing the influence of various solution components on the stability of binding solutions for molecular modification assays; see
The associated experiments were performed by varying the identity and/or concentration of components in a standard binding buffer (50 mM MES, 500 mM NaCl, 0.1% BSA, pH 5.0) to assess their effects on the stability of the binding solution. Stability was assessed by diluting binding reagent (120 mM gallium chloride) 1:400 (to 300 μM) in each of the indicated buffers, and then incubating 23 hours before performing a binding assay to measure PKA activity. As a control, the binding reagent was diluted 1:400 to create a binding solution and then used immediately (“fresh”) to measure PKA activity. The components that were varied include buffer (sodium acetate (NaAc) or 2-(4-morpholino)-ethane sulfonic acid (MES)), salt (500 mM NaCl), and protein (bovine serum albumin; BSA). The assay system included or lacked a protein kinase A (PKA) enzyme (“plus E” and “no E”, respectively). The assay system included a corresponding phosphorylation substrate, PKAtide (SEQ ID NO:20). Fluorescence polarization (mP) was measured 30 minutes after addition of the binding solution to the enzyme reaction.
These experiments show that BSA lowers the stability of the binding solution and the “active” concentration of gallium (possibly by complexing gallium). These experiments also show that NaCl has a beneficial effect on the total signal in this system.
Example 27This example describes the influence of various binding solutions on fluorescence polarization in molecular modification assays involving peptide substrates with no carboxy groups; see
Peptide substrates that lack carboxy groups may have lower affinities for a binding partner, such as gallium ions (Ga(III)), than peptide substrates having one or more carboxy groups. Thus, to engineer assays so that approximately equal percentages of both “low” and “high” affinity substrates (including fewer and more carboxy groups, respectively) bind to the binding partner, it may be helpful to use higher concentrations of binding sites with lower-affinity substrates. Specifically, it may be helpful to increase the number of (or flood the system with) binding sites of a binding partner to compensate for lower affinity peptides and/or (in the case of CDK) the need for higher concentrations of ATP. Unfortunately, although BSA may be the main cause of precipitation in the binding solution, high bead concentrations (e.g., to 1:100) also may lead to slight precipitation, especially with acetate-based buffer systems. Therefore, to have a relatively high concentration of gallium but to maintain a relatively low concentration of beads, the composition of the system may be adjusted by maintaining the same bead and detergent (e.g., Brij-35) concentrations, while increasing (e.g., by about fourfold in some embodiments) the gallium concentration.
This example describes the influence of various binding solutions on fluorescence polarization in molecular modification assays involving peptide substrates with one carboxy group; see
This example describes the influence of various binding solutions binding signals in molecular modification assays involving peptide substrates with two carboxy groups; see
To address the issue of peptides producing a high background in binding assays, experiments were performed to test the hypothesis that if the high background is due to nonspecific interaction of the substrate carboxy groups (acidic residues) with the binding partner, then it might be possible to lower that non-specific interaction by flooding the system with carboxy groups. This should be possible simply by raising the acetate concentration or by using another carboxy-containing buffer.
These graphs suggest that there may be a maximum recommended gallium concentration even if the background does not increase substantially. In particular, these graphs shows a bell-shaped “hump” that is dependent on the concentration of gallium used, which is worse in peptides with a higher background response to gallium (at n=2), and that it can be diminished (as well as the ATP resistance raised) by elevating the acetate concentration in the binding solution. This “hump” phenomenon is not affected by sodium chloride in the binding solution (data not shown).
Example 30This example describes the influence of various binding solutions on fluorescence polarization in molecular modification assays involving peptide substrates with three carboxy groups; see
Exemplary peptides in this group include the p38 substrate peptide, which is EGF receptor-derived (SEQ ID NO:9). This peptide may be a special case because it is labeled with fluorescein on the epsilon-NH2 group of the C-terminal lysine. This labeling scheme puts the 5FAM (5-carboxyfluorescein) label at the end of a flexible chain, which could increase the “wobble” factor of the fluorophore and thus reduce the maximum achievable fluorescence polarization. The C-terminus of the corresponding calibrator (phosphopeptide) is amidated, while the substrate C-terminus is not. Thus, there is a slight difference in the peptides being compared.
This example describes the influence of various binding solutions on the binding signals produced by peptide substrates with four or more carboxy groups; see
FIG. 39A,B shows graphed results from binding reactions performed on a Syk kinase assay (1 U/mL) with the original Zap 70 substrate peptide (“old peptide”; SEQ ID NO:24) in comparison with a modified Zap 70 substrate peptide (SEQ ID NO:23) having only two acidic residues (“new peptide” or “kit peptide”). Binding reactions included the indicated concentrations of acetate, 100 μM gallium chloride (a 1:800 dilution), and the presence or absence of 500 mM NaCl. Alternatively, binding reactions included the “kit” binding buffer (see Example 27). Binding reactions were incubated overnight with substrate peptide or product phosphopeptide and then the fluorescence polarization was measured. By raising the acetate concentration to 500 mM and lowering the gallium concentration to 100 μM, an assay window of 250 mP could be achieved.
This example describes binding assays testing the background fluorescence polarization signal for various exemplary peptide substrates as a function of gallium concentration; see
Binding reactions were performed with the indicated set of gallium concentrations for the following peptides (see Tables 5 and 6 for sequences and additional identifying information): Abl/Arg substrate peptide (SEQ ID NO:2), Alpha tubulin-derived peptide (SEQ ID NO:23), CHK1tide (SEQ ID NO:4), CHK2 tide (SEQ ID NO:5), Crosstide (SEQ ID NO:6), EGF-R der. peptide (SEQ ID NO:9), GS der. peptide (SEQ ID NO:11), CDKtide (SEQ ID NO:13), Lyn/Blk (SEQ ID NO:16), Src (SEQ ID NO:17), PKA tide (SEQ ID NO:20), and S6 ribosomal (SEQ ID NO:21). The background binding signal produced at higher gallium concentrations varied substantially according to peptide sequence. In particular, peptides with two or more carboxy groups tend to give higher background that those with one or fewer carboxy groups.
The following table summarizes correlations between gallium sensitivity and the charge (titrated, theoretical, number of acidic, etc.) of various peptides described herein.
This example describes data obtained with different acetate concentrations to assay different peptides.
Additional experiments were performed using a third set of buffers based on acetate which differed primarily in acetate concentration. A binding buffer (Buffer 1) comprising 50 mM acetic acid, 500 mM NaCl, and titrated to pH 5.0 with NaOH, was used to dilute a stock solution of GaCl3 (0.48M in 0.1 M HCl) 1:400. This binding solution provided good discrimination in binding to 100 nM FL-Phosphocrosstide (SEQ ID NO:7) but not 100 nM FL-Crosstide (SEQ ID NO:6).
This binding solution did not give good results for another peptide/phosphopeptide pair based on the fluorescein-labeled α-tubulin-derived substrate (SEQ ID NO:23). A modification of the binding buffer provided better results. A 290 mM acetate binding buffer was made by mixing 75 parts of Buffer 1 with 25 parts of a second buffer (Buffer 2, comprising 1 M acetic acid, titrated to pH 5.0 with NaOH). The above-mentioned GaCl3 solution was diluted into this 290 mM acetate buffer 2400 fold. The results demonstrate that the tubulin-derived peptide substrate works much better in this high-acetate system. Accordingly, a ratio of buffers 1 and 2, or a buffer or salt concentration, may be selected according to the sequence and/or charge of the binding target (e.g., peptide).
Example 34This example summarizes observations on assay components and assay performance from data presented in Examples 26-33.
The influence of a variety of assay components and conditions on assay performance has been evaluated. To facilitate this evaluation, the assay systems sometimes have been characterized based on some set of suitable parameters (e.g., related to reaction equilibrium). For example, the peptide/enzyme systems used in kinase/phosphatase assays have been characterized using (1) the Km (Michaelis-Menten constant) of the substrate with a particular kinase, (2) the affinity of the substrate in the nonphosphorylated state to the binding entity (“background”), and (3) the affinity of the substrate in the phosphorylated state to the binding partner (absolute, and in relationship to the amount and affinity of ATP used). The following observations are noted here, based in part on these parameters:
1. Bovine serum albumin (BSA) in the binding solution may (a) enhance precipitation dramatically, and (b) may reduce the concentration of “active” gallium.
2. Binding solution stability may be comparable or the same in each system. Typically, the number of binding sites will decrease over time. However, whether this decrease becomes apparent is a function of the peptide sequence and the ATP concentration used.
3. Gallium concentration is the main contributing factor for the binding capacity of the binding solution.
4. The preferred or usable assay window lies between specific and nonspecific binding of the phosphorylated peptide to the binding entity. This window may be defined by the concentration of gallium, acetate, NaCl, and the number of free carboxy groups in the substrate.
5. Acetate (e.g., in the form of sodium acetate buffer) can be used as a source of carboxy groups to “block” nonspecific binding of acidic residues of peptides to binding partners.
6. The stability of the binding solution may be increased by a decrease in pH from 5.5 to 5.0. This change also puts the pH of the binding solution closer to the pKa of acetate and thus improves the buffering capacity of the system.
7. The peptide substrates roughly can be categorized by counting free carboxy groups (acidic residues+carboxy-terminus). The carboxy-terminus may not be considered free (and thus not counted) if it is modified to remove its negative charge (such as by amidation).
Example 35This example generalizes the observations of Examples 26-34 to produce general rules for creating binding buffers for molecular modification assays.
These observations have a number of implications for assay construction. These implications may address the stability of the binding solution, the influence of acidic residues of the peptide substrate (or product) on assay results, and the amount of ATP that may be used in assays, among others. These implications may be interpreted using any suitable criteria, including the 24-hour stability of the binding solution and/or the ATP resilience, among others. Selected implications may include:
1. BSA in the binding buffer may be the main reason for the instability and precipitation in the binding solution. Reformulated binding buffer (no BSA; pH 5.0; and varied amounts of gallium (from the binding reagent), NaAc, and NaCl) may result in a more stable binding solution (e.g., as determined 24 hours after preparation at room temperature).
2. The assay window of any given peptide/phosphopeptide pair can be adjusted by changing the concentration of NaAc, NaCl, and gallium in the binding solution. The optimum concentrations are influenced mainly by the total number of carboxy groups in the substrate (on luminophore, number of acidic amino acids, C-terminus (amidated or free), etc.). Secondary considerations include the type of acidic amino acid, positioning of carboxy groups (relative to each other and to the phosphorylation site), and luminophore type. Knowing these features allows the buffer system to be optimized for each application, if desired. These observations and insights virtually remove sequence restrictions on the substrates and vastly improve ATP tolerance, especially for peptides with no or few acidic residues.
3. The reformulated “high” gallium binding reagent makes it possible to have very high gallium concentrations in binding solution and thus further improves ATP tolerance in the system.
4. The change of carrier substance in reaction buffer can be beneficial for inhibitor 1050 determination, depending on the inhibitor. Several proteins and detergents (at least) are compatible.
These findings for different assays can be summarized in tabular form, based on the number of carboxy groups in the peptide substrate.
The assays more generally can be performed using any suitable components and conditions. Thus, components generally may be replaced by other components capable of performing the same or similar functions. For example, in this context, acetate could be replaced by propionate or other suitable compositions, among others.
Example 36This example describes further generalizations about adjusting binding solutions in molecular modification assays according the structure and/or charge of the binding target.
The following table summarizes exemplary starting conditions for optimization of binding solutions according to the structure and/or charge of the binding target (such as target peptide). The binding solutions may include the indicated ratios of Buffer 1 (50 mM acetic acid, 500 mM NaCl, titrated to pH 5.0 with NaOH) and Buffer 2 (1M acetic acid, titrated to pH 5.0 with NaOH). The binding solutions also may include the indicated dilutions of a gallium-based binding reagent (such as gallium chloride at 0.48M). Binding reactions may be incubated with binding targets for the indicated times before measuring a binding signal (such as fluorescence polarization). The time of incubation may be selected according to the structure/charge of the target peptide and/or according to the binding solution used in the binding reaction.
This example describes aspects of the present teachings relating to resonance energy transfer, including using FRET (Fluorescence Resonance Energy Transfer) and TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) as a detection mechanism; see
FRET (or TR-FRET) can be used as a readout or reporter function in any of the assays described herein, including but not limited to kinase assays (as described immediately above), phosphatase assays, cyclase assays, and phosphodiesterase assays, among others. In each case, one member of a FRET donor/acceptor pair may be associated with the substrate and/or product, and one member may be associated with the binding partner. For example, a FRET phosphatase assay may be performed essentially as described above, beginning with a phosphorylated substrate, where phosphatase activity will result in a loss in energy transfer as phosphorylated substrate is converted to nonphosphorylated product (with a resulting loss of affinity for the binding partner). More generally, the donor and acceptor may be positioned on any suitable reaction components, at any suitable point(s) in the reaction, as long as the reaction leads to a detectable change in energy transfer.
The donor and acceptor may be associated with reaction components using any suitable mechanism(s). For example, the association may be covalent or noncovalent, direct or indirect, and so on. In
FRET-based assays may have various advantages over (or in combination with) other assay formats, for example, fluorescence-polarization (FP) based formats. First, FP-based assays may work best with relatively high substrate conversion, which in turn effectively may place an upper limit on usable substrate concentration and/or necessitate use of higher enzyme concentrations. FRET-based assays may overcome these potential limitations. Second, FP-based assays may be limited by the size of the luminescent entity (unless labeled by long lifetime fluorophores). TR-FRET does not have this size limitation. Third, although both IMAP-FP and the TR-FRET assays work well under standard IMAP-FP conditions, TR-FRET will gain in the sensitivity of its response with increasing substrate concentrations (because the assay only responds to product, not substrate; hence, more substrate=more product at any fixed kinase concentration), whereas, in FP, the opposite occurs (because greater product formation is obscured by increasing substrate concentration). Thus, FRET may provide greater flexibility in choice of substrate concentrations, as well as the ability to use lower enzyme concentrations. Moreover, substrates that are too poor to use in IMAP might very well be used in the TR-FRET assay. Fourth, FP and TR-FRET both may be read in the same system. Thus, information from both assays can be combined to enhance precision and reduce interferences, further than either technique can do alone. Fifth, FRET assays may be multiplexed, for example, using multiple acceptors such as TAMRA and fluorescein on separate peptides for separate kinases.
The FRET approach generally involves constructing an assay so that changes in donor-acceptor separation, detectable as changes in energy transfer, are correlated with changes in the assay parameter(s) of interest. These parameters may include the presence or activity of a species of interest, the kinetics and/or endpoint of a reaction of interest, and so on. This change in separation may be effected using any suitable mechanism(s), consistent with these goals. For example, in the metal-based embodiments described herein, a donor (e.g., lanthanide salt) may be brought directly into proximity with gallium (e.g., in mixed clusters), and FRET detected in the presence of an acceptor-labeled phosphorylated entity that interacts with the gallium. Alternatively, or in addition, the donor and acceptor may be brought into (or out of) contact via interaction with a common binding partner. (For example, one member of a donor/acceptor pair may always interact with the common binding partner, and the second member of the donor/acceptor pair may be brought into (or out of) contact based on the outcome of a reaction.) This indirect approach is the same, in a sense, as the direct approach, in that it allows for proximity between a phosphorylated acceptor-labeled entity, such as a peptide, a lanthanide compound (cryptate), and the gallium binding entity, bringing donor and acceptor together. However, this indirect approach is different, in a sense, in that it allows associating the lanthanide entity in a better-defined way with the gallium binding entities (e.g., onto their surface). In some embodiments, this association may involve a cryptate, and not the metal ion itself, which may increase brightness of the lanthanide and shield the lanthanide from interactions with the gallium itself. Binding of donor and acceptor to a common binding partner may amplify signal, but bringing more than one donor and/or acceptor into proximity, enhancing the avenues for and thus the likelihood of energy transfer. These concepts are illustrated in
In this approach, the number of phosphate binding sites, the concentration of acceptor, and the concentration of donor can be modified independently from each other, which may provide big advantages in FRET (or TR-FRET) assays. This may be especially true for kinase assays, where the phosphate binding capacity must enable the interaction of donor and acceptor, and at the same time be in excess over the amount of ATP added. In addition, this approach may allow modification of the size of the gallium binding entities, and thus the number of gallium binding sites, by modifying pH (and/or other solution properties), and with that possibly enhancing the possibility of proximity between donor and acceptor.
The FRET-based assay approach may include a variety of embodiments and improvements. For example, a phosphorylated peptide/protein/molecule may be labeled with lanthanide (Eu(III) or Tb(III)) cryptate or chelate to tether it on the surface of a gallium binding entity. Alternatively, or in addition, Tamra (Tb) or Cy5 (Eu) or others may be used as acceptors on an enzyme substrate. Alternatively, or in addition, the lanthanide may be tethered onto the enzyme substrate, and the acceptor onto the gallium binding entity (essentially reversing some of the embodiments described above). The acceptor in this case could be APC (tethered by non-specific interaction) APC is a superior acceptor to Cy5, although nonspecific interaction may not be a good tether mechanism for a general assay principle. Alternatively, or in addition, assays may involve non-lanthanide donors, such as quantum dots (Q-dots), or standard fluorophore pairs, such as fluorescein and TAMRA, although such donors and donor/acceptor pairs may make it more difficult or impossible to reduce background using time-resolved FRET (as possible with lanthanide compounds and other long-lifetime donors).
Example 38This example describes further selected aspects and embodiments of the present teachings, including experimental setups, results, and related discussion, as set forth in the Appendix of U.S. Provisional Patent Application Ser. No. 60/615,308, filed Sep. 30, 2004, which is incorporated herein by reference.
Example 39This example describes exemplary lanthanide chelates, suitable for use as members of a resonance energy transfer pair, in accordance with aspects of the present teachings. The chelates optionally may include a reactive group, a member of a specific binding pair, and so on, to facilitate interaction with a substrate, product, binding partner, and/or other reaction component. Here, the chelates include a phosphate group, to facilitate interaction with suitable metals associated with a binding partner. The chelates may function to stabilize the Tb(III) and shield it from deactivation by water. The chelates may include antenna portions, which act as light collecting devices to relay the energy to the Tb(III) core, improving luminescence. Tb and other lanthanides have long fluorescence lifetimes (300 μsec−1 msec) relative to “standard” luminophores such as FAM and TAMRA (nsec). By introducing delay (50 msec) between excitation and measurement of emission, non-FRET acceptor fluorescence and background sample autofluorescence may be reduced or avoided.
The exemplary chelates may be characterized schematically, for example, using three general formulae:
The “sensitizer” generally comprises any structure capable of and/or adapted to enhance light absorption, including but not limited to heterocyclic aromatic compounds, such as 2- or 4-quinolones, 2- or 4-coumarins, phenones, quinolines, and merocyanines, among others. These structures may be selected from the following groups, among others:
The “tether” generally comprises any structure capable of and/or adapted to join portions of the chelate, including but not limited to —(CH2)nO—, —(CH2)nPhO—, —(CH2)nPh(CH2)mO—, carboxyalkoxy, and alkenoxy, among others. Here, n and m are independently 1-12, R1 is independently H or alkyl, and Ph is a phenyl group.
The exemplary chelates may be synthesized via any suitable mechanism, for example, by introducing a phosphate group onto the skeleton of a lanthanide chelate. Thus, in a representative synthetic pathway, the carbostyril 124 residue of cs124-DTPA (diethylene triamine pentaacetate) may be modified, as shown:
A phosphate group may be introduced onto another portion of cs24-DTPA, via an appropriate linkage.
A laser dye, such as merocyanine, may be used as a sensitizer.
Finally, a linkage may be introduced onto a skeleton of DPTA.
This example describes additional exemplary lanthanide chelates, suitable for use as members of a resonance energy transfer pair, in accordance with aspects of the present teachings.
The luminescent lanthanide chelate may be characterized by a number of parameters, including extinction coefficient, quantum yield, and luminescence lifetime. Extinction coefficient is a wavelength-dependent measure of the absorbing power of a luminophore. Quantum yield is a ratio of the number of photons emitted to the number of photons absorbed by a luminophore. Luminescence lifetime is the average time between absorption and re-emission of light by a luminophore. Lanthanide luminescence is typically exceptional for its long luminescence lifetimes, which often are in the microsecond to millisecond range.
Luminescent lanthanide complexes generally include a luminescent trivalent lanthanide atom and an organic chelator bound to the trivalent lanthanide. The organic chelator may be used to fine-tune the spectral properties of the lanthanide and to permit the lanthanide to participate in specific interactions with biological molecules. The chelator may effectively increase the extinction coefficient of the lanthanide by acting as an “antenna” or “sensitizer” that can absorb light and transfer the associated energy to the lanthanide ion. The chelator also may increase the quantum yield of the lanthanide by decreasing luminescence quenching by the solvent.
The spectral properties of photoluminescence may be characterized by excitation spectrum, emission spectrum, and/or Stokes' shift, among others. An excitation spectrum is the dependence of emission intensity upon the excitation wavelength, measured at a single constant emission wavelength. An emission spectrum is the wavelength distribution of the emission, measured after excitation with a single constant excitation wavelength. A Stokes' shift is the difference in wavelengths between the maximum of the emission spectrum and the maximum of the absorption spectrum.
Luminescence-based methods or assays may be influenced by the parameters discussed above—extinction coefficient, quantum yield, luminescence lifetime, excitation and emission spectra, and/or Stokes' shift, among other—and may involve characterizing luminescence intensity (e.g., FLINT), luminescence polarization or anisotropy (e.g., FP), luminescence resonance energy transfer (e.g., FRET), luminescence lifetime (e.g., FLT), total internal reflection luminescence (e.g., TIRF), luminescence correlation spectroscopy (e.g., FCS), and/or luminescence recovery after photobleaching (e.g., FRAP or FPR), among others.
Luminescence methods have several significant potential strengths. For example, luminescence methods may be very sensitive, because modern detectors, such as photomultiplier tubes (PMTS) and charge-coupled devices (CODs), can detect very low levels of light. In addition, luminescence methods may be very selective, because the luminescence signal may come almost exclusively from the luminophore.
The present teachings provide systems, including compositions, kits, and methods, particularly for photoluminescence applications. The compositions and kits may include organic chelators, luminescent lanthanide complexes that incorporate those chelators for use in certain photoluminescence assays, and/or precursors and derivatives of these chelators and complexes, among others. The methods may involve detecting light emitted by the complex, and using properties of that light to understand properties of the complex and its environment. Thus, in this aspect, the compositions may act as reporter molecules, for example, to report on the activity of an enzyme and/or a modulator, such as an agonist or antagonist, of the enzyme.
The organic chelator may be a derivative of a polyazamacrocyclic chelating group. For example, the organic chelator may be a derivative of a 1,4,7,10-tetraazacyclododecane ring system, for example, having the formula:
The tetraazacyclododecane chelator may be substituted. For example, the chelator may be substituted at the 1-, 4-, and 7-positions by substituents R1, R2, and R3. The chelator further may be substituted at the 10-position by a sensitizer Z. The sensitizer, as discussed in the Introduction, may act as an antenna that increases luminescence by capturing and transferring light energy to an associated lanthanide. The sensitizer typically comprises a polyheterocyclic ring system.
Substituents R1, R2, and R3, which may be the same or different, may be hydrogen, or may be a substituent selected to facilitate the binding of a lanthanide ion within the tetraazacyclododecane ring. Substituents R1, R2, and R3 typically incorporate functional groups that help complex the selected lanthanide. For example, the R1, R2, and R3 substituents may incorporate substituents that themselves incorporate a carbonyl group, such as a carboxylic acid, ester, or amide. Alternatively or in addition, the R1, R2, and R3 substituents may incorporate a phosphonate moiety.
The R1, R2, and R3 substituents are typically acetic acid derivatives, which may be substituted or unsubstituted, as shown below:
Here, the R4, R5, and R6 substituents may be independently hydroxy, alkyl groups having 1-6 carbons, alkoxy groups having 1-6 carbons, or amine groups, each of which optionally may be further substituted by additional aliphatic groups, aromatic groups, amide groups, and/or heteroatom-substituted aliphatic groups.
The sensitizer Z may comprise a polycyclic heteroaromatic ring system that is bound to the tetraazacyclododecane ring via a covalent linkage. The heteroaromatic ring system may include 2-6 fused aromatic rings, having 1-6 heteroatoms. In some aspects of the chelator, the sensitizer has the formula
where K is a covalent linkage to the tetraazacyclododecane, and X, Y, V, and J are carbon or a heteroatom that is nitrogen, oxygen, sulfur, or selenium. The sensitizer optionally may be further substituted at one or more positions by additional substituents, such as alkyl, alkoxy, halogen, carboxylic acid, sulfonic acid, and/or phosphonate, among others. Typically, the linkage K is an alkyl linkage, and more typically K is a methylene group. In one particular aspect of the chelator, X is nitrogen, V is an aromatic carbon, and both J and Y are oxygen. Particularly preferred sensitizers have the formula
where Y is oxygen, sulfur, or selenium
The chelator typically forms a complex with a lanthanide ion so that at least the nitrogen atoms of the tetraazacyclododecane macrocycle bind to the lanthanide ion. Typically one or more of substituents R1, R2, and R3 also may coordinate with the lanthanide ion, as may a heteroatom present in the sensitizer Z. Where the resulting lanthanide ion is not fully complexed, the remaining coordination sites may be occupied by a solvent molecule, such as water, or by one or more additional ligands that may be strongly coordinated to the lanthanide ion, or may be subject to ligand exchange when the complex is in solution.
The complexed lanthanide ion may be selected from cerium (Ce), praseodymium (Pr), neodymium (Nd), promethium (Pm), samarium (Sm), europium (Eu), gadolinium (Gd), terbium (Tb), dysprosium (Dy), holmium (Ho), erbium (Er), thulium (Tm), ytterbium (Yb), and lutetium (Lu). Typically, the lanthanide ion is terbium, europium, dysprosium, or samarium. More typically, the lanthanide is terbium.
In a particularly preferred embodiment, the lanthanide complex (including chelator, sensitizer, and lanthanide) has the formula
The chelator optionally may be further substituted by one or more reactive functional groups (RX), or conjugated substances (SC), each of which is bound to the chelator via a covalent linking moiety L. The reactive functional group or conjugated substance may be a substituent on the polyazamacrocycle itself, a substituent on R1, R2, or R3, or a substituent on the sensitizer Z. Where the lanthanide complex is substituted by a reactive functional group, it is typically a substituent on the sensitizer moiety.
The covalent linking moiety L is optionally a single covalent bond, such that either the reactive functional group RX or the conjugated substance SC is bound directly to the organic chelator. Alternatively, L may incorporate a series of nonhydrogen atoms that form a stable covalent linkage between the reactive functional group or conjugated substance and the chelator. Typically, L may incorporate 1-20 nonhydrogen atoms in a stable conformation. Stable atom conformations include, without limitation, carbon-carbon bonds, amide linkages, ester linkages, sulfonamide linkages, ether linkages, thioether linkages, and/or other covalent bonds. Preferred covalent linkages may include single bonds, carboxamides, sulfonamides, ethers, and carbon-carbon bonds, or a combination thereof.
The reactive functional group RX may include any functional group that exhibits appropriate reactivity to be conjugated with a desired substance. The choice the reactive group typically depends on the functional groups present on the substance to be conjugated. Typically, functional groups present on such substances include, but are not limited to, alcohols, aldehydes, amines, carboxylic acids, halogens, ketones, phenols, phosphates, and thiols, or a combination thereof. Suitable RX groups include activated esters of carboxylic acids, aldehydes, alkyl halides, amines, anhydrides, aryl halides, carboxylic acids, haloacetamides, halotriazines, hydrazines (including hydrazides), isocyanates, isothiocyanates, maleimides, phosphoramidites, sulfonyl halides, and thiol groups, or a combination thereof. Typically, RX is an activated ester of a carboxylic acid, an amine, a haloacetamide, a hydrazine, an isothiocyanate, or a maleimide group. In one aspect of the lanthanide complex, RX is a succinimidyl ester of a carboxylic acid.
The organic chelators that are substituted with a reactive functional group may be used to prepare a variety of conjugates. The conjugated substance may be a member of a specific binding pair. Alternatively, the conjugated substance may be a molecular carrier. The conjugated substance may include a biomolecule that is an amino acid, a peptide, a protein, a nucleoside, a nucleotide, an oligonucleotide, a nucleic acid polymer, or a carbohydrate. The conjugated substance may include a polar moiety, or a masked polar moiety, or the conjugated substance may include a solid or semi-solid matrix. The conjugated substance may include one or more additional dyes or luminophores.
The conjugated substance SC may be a naturally occurring or a synthetically modified substance, particularly where it is an amino acid, a peptide, a protein, a nucleoside, a nucleotide, an oligonucleotide, a nucleic acid polymer, or a carbohydrate. For example, the conjugated substance may be a polypeptide or other substance that is naturally or artificially substituted by one or more phosphate functional groups. In this example, the conjugated substance may be substituted by phosphate prior to conjugation with the organic chelator, or the selected conjugated substance may first be conjugated to the organic chelator, and subsequently phosphorylated, for example, by enzymatic phosphorylation.
The conjugated substance SC also may be a member of a specific binding pair or a molecular carrier. Specific binding pair members typically specifically bind to and are complementary with the complementary member of the specific binding pair. Conjugated members of a specific binding pair can be used to localize compounds of the present teachings to the complementary member of that specific binding pair. Representative specific binding pairs are listed in Table 11.
The conjugated substance SC may be a biological or artificial polymer, particularly where it is a carrier. Biological polymers include proteins, carbohydrates, and nucleic acid polymers. Artificial polymers include polyethylene glycols and polymeric microparticles composed of polystyrene, latex, or other polymeric material. Preferably, a conjugated carrier is a carbohydrate that is a dextran, or amino-substituted dextran, or a polymer microparticle. The conjugated carrier may be selected so that conjugation of the lanthanide complex to the carrier detectably alters one or more luminescence properties of the complex. In particular, the carrier may be selected so that conjugation of the lanthanide complex to the carrier alters the fluorescence intensity or polarization of the lanthanide complex.
The conjugated substance SC may be a metal or glass surface, and may be, for example, the sides or bottom of a microwell, or a slide, or the surface of a chip, particularly where the conjugated substance is a solid or semi-solid matrix. The compound of the present teachings is optionally covalently bound to a fiber optic probe, where the probe is composed of glass or functionalized glass (e.g., aminopropyl glass), or the compound is attached to the fiber optic probe via an intermediate polymer, such as polyacrylamide. Incorporation of the compounds of the present teachings on such surfaces permit the remote sensing of sample pH values.
The conjugated substance SC may include or comprise a dye or luminophore. In such cases, the dye or luminophore may be selected so that energy-transfer occurs between the lanthanide complex and the conjugated dye or luminophore, where the lanthanide complex is optionally the luminescence donor, or the luminescence acceptor.
Example 41This example describes, without limitation, exemplary prenyl transfer assays, in accordance with aspects of the present teachings. Prenyl transfer activity may include addition (prenylation) or removal (deprenylation) of prenyl groups, such as farnesyl or geranylgeranyl groups, among others. The prenyl groups may be added to (or removed from) any suitable molecules, such as polypeptides, among others, in prenyl transfer assays. In some examples, the prenyl transfer assays may include an acceptor substrate, a donor substrate, a sample, and an association partner. The acceptor substrate may be configured to receive a prenyl group from the donor substrate, to produce a product by action of a prenyl transfer activity, such as a transferase enzyme, in the sample. The association partner may associate selectively with the product relative to at least one of the substrates, or with at least one of the substrates relative to the product. Furthermore, the product, one or more of the substrates, and/or the association partner may include a luminescent label. Accordingly, the extent of selective association with the association partner, and thus the amount of prenyl transfer activity, may be measured in a luminescence assay, such an assay detecting luminescence polarization, luminescence energy transfer, and/or luminescence intensity, among others. In some examples, candidate modulators of prenyl transfer activity may be screened for their ability to affect (e.g., inhibit) prenyl transfer activity. The systems of the present teachings may provide more rapid, sensitive, safe, and/or effective approaches to detecting prenyl transfer activity. These systems thus may be useful in a variety of applications, including, without limitation, life science research, drug research, accelerated drug discovery, assay development, and/or high-throughput screening. Further aspects of prenyl transfer assays are described in U.S. patent application Ser. No. 10/957,332, filed Sep. 30, 2004, which is incorporated herein by reference.
Example 42This example describes, without limitation, exemplary polypeptide modification assays, in accordance with aspects of the present teachings. These assays may be used for detecting molecular modifications of molecules such as polypeptides, among others, and the presence and/or activity of enzymes and/or other agents involved in facilitating or otherwise regulating such modifications. The molecular modifications may include the cleavage or degradation of molecules such as polypeptides, among others. However, the molecular modifications also may include splicing of two molecules, such as by protein ligases, including ubiquitin-protein conjugations (or ubiquitination), among others. The present teaching may include a method of detecting modification of a polypeptide in a sample, where the method includes contacting the sample with a binding partner that binds specifically to one of the modified and nonmodified forms of the polypeptide, but not both, the binding partner including a metal required for specific binding to the one form; and detecting a response indicative of the extent of binding between the binding partner and the one form. The assays may include luminescence assays, such as luminescence polarization, luminescence resonance energy transfer, and/or luminescence intensity, among others. The assays provided by the present teaching may be useful in a variety of applications, including without limitation life science research, drug research, accelerated drug discovery, assay development, and high-throughput screening. Further aspects of lipid assays are described in U.S. Patent Application Ser. No. 60/554,766, filed Mar. 19, 2004, which is incorporated herein by reference.
Example 43This example describes, without limitation, exemplary lipid modification assays, in accordance with aspects of the present teachings. These assays may be used for detecting molecular modifications, such as phosphorylation, dephosphorylation, and/or cleavage, among others, of lipids, lipid fragments, and/or lipid precursors. These assays also may be used to detect the presence and/or activity of enzymes and/or other agents, such as drugs, involved in facilitating, inhibiting, or otherwise regulating such lipid modifications. The molecular modifications may include structural changes in lipids, lipid fragments, and/or lipid precursors, such as phosphate addition and/or removal, among others. Alternatively, or in addition, the molecular modifications may include cleavage of a lipid into lipid fragments, such as by a lipase enzyme, and/or joining of two lipid precursors, such as by a lipid ligase enzyme, among others. The present teachings may include a method of detecting modification of a lipid (and/or lipid fragment and/or lipid precursor) in a sample, where the method includes contacting the sample with a binding partner that binds specifically to one of the modified and nonmodified forms of the lipid (and/or fragment and/or precursor), but not both, the binding partner including a metal required for specific binding to the one form; and detecting a response indicative of the extent of binding between the binding partner and the one form. The assays may include luminescence assays, such as luminescence polarization, luminescence resonance energy transfer, and/or luminescence intensity, among others. The assays provided by the present teachings may be useful in a variety of applications, including, without limitation, life science research, drug research, accelerated drug discovery, assay development, and high-throughput screening. Further aspects of lipid assays are described in U.S. patent application Ser. No. 11/146,553, filed Jun. 6, 2005, which is incorporated herein by reference.
Example 44This example describes selected aspects and embodiments of the present teachings, as a first series of ordered paragraphs.
1. A method of detecting the activity of an enzyme that operates on an enzyme substrate to form an enzyme product in a sample, comprising:
contacting the substrate or product with a binding partner that specifically binds to the substrate or to the product but not to both, where the binding partner includes a metal that is involved in binding between the binding partner and the substrate or product;
contacting the substrate with the enzyme;
exposing the sample to light capable of inducing luminescence from the sample; and
measuring a detectable luminescence energy transfer response, without separating the bound substrate or product from the unbound substrate or product, where the detectable luminescence energy transfer response is indicative of the extent of binding between the substrate or product and the binding partner; and
correlating the luminescence energy transfer response with the activity of the enzyme.
2. A method of detecting phosphorylation or nonphosphorylation of a polypeptide in a sample, comprising:
contacting the polypeptide with a binding partner that specifically binds to the phosphorylated polypeptide or to the nonphosphorylated polypeptide but not to both, where the binding partner includes a metal that is required for binding between the binding partner and the phosphorylated polypeptide or nonphosphorylated polypeptide;
exposing the sample to light capable of inducing luminescence from the sample; and
measuring a detectable luminescence energy transfer, where the luminescence energy transfer is indicative of the extent of binding between the polypeptide and the binding partner without separating the bound polypeptide from the unbound polypeptide; and
correlating the luminescence energy transfer with the extent of phosphorylation or nonphosphorylation of the polypeptide, or with the activity of an enzyme that affects phosphorylation or nonphosphorylation of the polypeptide.
3. A method of detecting cyclization or noncyclization of a nucleotide in a sample, comprising:
contacting a nonradioactive nucleotide with a binding partner that specifically binds to a cyclized nucleotide or to a noncyclized nucleotide but not to both, substantially without regard to the nucleoside portion of the nucleotide, where the binding partner includes a metal that is required for binding between the binding partner and the cyclized nucleotide or noncyclized nucleotide;
exposing the sample to capable of inducing luminescence from the sample; and
measuring a detectable luminescence energy transfer, where the luminescence energy transfer is indicative of the extent of binding between the nucleotide and the binding partner; and
correlating the response with the extent of cyclization or noncyclization of the nucleotide, or with the activity of an enzyme that affects cyclization or noncyclization of the nucleotide.
4. The method of one of paragraphs 1, 2, or 3, where the metal is a tricationic metal ion.
5. The method of paragraphs 1, 2, or 3, where the metal is selected from the group consisting of aluminum, iron, gallium, europium, and terbium.
6 The method of paragraph 5, where the metal is Ga(III).
7. The method of one of paragraphs 1, 2, or 3, where the binding partner further includes a dicationic metal ion.
8. The method of paragraph 2, where at least one of the phosphorylated polypeptide, nonphosphorylated polypeptide, and the polypeptide is luminescent.
9. The method of paragraph 3, where at least one of the cyclized nucleotide, noncyclized nucleotide, and the nucleotide is luminescent.
10. The method of one of paragraphs 1, 2, or 3 where measuring the detectable luminescence energy transfer response includes measuring luminescence intensity.
11. The method of one of paragraphs 1, 2, or 3, where the detectable luminescence energy transfer is detectable luminescence resonance energy transfer.
12. The method of paragraph 1, where at least one of the substrate and/or product, and the binding partner includes an energy transfer donor, and at least the other of the substrate and/or product, and the binding partner includes an energy transfer acceptor, such that luminescence energy transfer can occur between the donor and the acceptor.
13. The method of paragraph 2, where the binding partner includes a luminescent energy transfer donor, and at least one of the phosphorylated polypeptide, nonphosphorylated polypeptide, and polypeptide, is an energy transfer acceptor, such that luminescence energy transfer can occur between the energy transfer donor and the energy transfer acceptor.
14. The method of paragraph 3 where the binding partner includes a luminescent energy transfer donor, and at least one other of the cyclized nucleotide, noncyclized nucleotide, and nucleotide includes an energy transfer acceptor, such that luminescence energy transfer can occur between the energy transfer donor and the energy transfer acceptor.
15. The method of one of paragraphs 12, 13, and 14, where the binding partner includes an energy transfer donor.
16. The method of paragraph 12, where the binding partner includes an energy transfer donor or acceptor, and that energy transfer donor or acceptor includes the metal that is involved in binding between the binding partner and the substrate or product.
17. The method of paragraph 15, the energy transfer donor having a luminescence lifetime, where the luminescence lifetime is at least about 400 nanoseconds.
18. The method of paragraph 15, where the energy transfer donor comprises a lanthanide.
19. The method of one of paragraphs 12, 13, and 14 where the energy transfer donor is a luminescent lanthanide chelate.
20. The method of one of paragraphs 12, 13, and 14 where the energy transfer donor is a luminescent metal-ligand complex donor.
21. The method of paragraph 20 where the metal-ligand complex is a ruthenium complex, an osmium complex, a rhenium complex, a platinum complex, or an iridium complex.
22. The method of one of paragraphs 12, 13, and 14 where the energy transfer acceptor is a xanthene, a phenoxazine, a phthalocyanine, a cyanine, a porphyrin, a polyazaindacene, or a phycobiliprotein.
23. The method of paragraph 19, where the lanthanide chelate includes an organic chelator and a sensitizer moiety.
24. The method of paragraph 19, where the lanthanide chelate is associated with the binding partner via a metal-binding functional group that binds to the metal.
25. The method of paragraph 24, where the metal-binding functional group is a phosphate, sulfonic acid, or carboxylic acid functional group.
26. The method of paragraph 24, where the metal-binding functional group is a phosphate functional group.
27. The method of paragraph 24, where the metal-binding functional group is bound to the lanthanide chelate via a covalent linkage.
28. The method of paragraph 27, where the metal-binding functional group is bound to the sensitizer portion of the lanthanide chelate.
29. The method of paragraph 27, where the metal-binding functional group is bound to the organic chelate portion of the lanthanide chelate.
30. The method of paragraph 19, where the organic chelator has the structural formula of Formula I, Formula II, or Formula III
where the sensitizer moiety is a heterocyclic polyaromatic ring structure; and
where the tether moiety is a covalent linkage.
31. The method of paragraph 19, where the organic chelator has the formula
where the R4, R5, and R6 substituents are independently hydroxy, alkyl groups having 1-6 carbons, alkoxy groups having 1-6 carbons, or amine groups, each of which is optionally further substituted by additional aliphatic groups, aromatic groups, amide groups, and heteroatom-substituted aliphatic groups;
Z is a sensitizer moiety that is a polycyclic heteroaromatic ring system that is bound via a covalent linkage;
the lanthanide chelate is substituted by a metal-binding functional group; and
the lanthanide chelate is optionally substituted by one or more reactive functional groups or conjugated substances.
32. The method of paragraph 31, where Z is a sensitizer moiety having the formula
where K is the covalent linkage;
where X, Y, V, and J are carbon or a heteroatom that is nitrogen, oxygen, sulfur, or selenium; and
where the sensitizer is optionally further substituted at one or more positions by alkyl, alkoxy, halogen, or a metal-binding functional group.
33. The method of paragraph 19, where the lanthanide chelate includes a complexed lanthanide ion that is selected from cerium (Ce), praseodymium (Pr), neodymium (Nd), promethium (Pm), samarium (Sm), europium (Eu), gadolinium (Gd), terbium (Tb), dysprosium (Dy), holmium (Ho), erbium (Er), thulium (Tm), ytterbium (Yb), and lutetium (Lu).
34. The method of paragraph 19, where the lanthanide chelate includes a complexed lanthanide ion that is selected from terbium, europium, dysprosium, or samarium.
35. The method of paragraph 34, where the lanthanide ion is terbium.
36. The method of paragraph 19, where the lanthanide chelate has the formula
where the lanthanide chelate is associated with the binding partner via a metal-binding functional group, and is optionally further substituted by one or more reactive functional groups or conjugated substances.
37. The method of paragraph 1, where the substrate is a polypeptide, and where the substrate and product are related by a posttranslational modification.
38. The method of paragraph 37, where the posttranslational modification is phosphorylation or dephosphorylation of the polypeptide.
39. The method of paragraph 37, where the posttranslational modification is proteolysis or ligation of the polypeptide.
40. The method of paragraph 37, where the posttranslational modification is prenylation of the polypeptide.
41. The method of paragraph 1, where the substrate is a nucleotide, and where the substrate and product are related by a cyclization or decyclization of the nucleotide.
42. The method of paragraph 1, where the substrate is a lipid, lipid precursor or lipid fragment.
43. The method of paragraph 1, where the enzyme is selected from the group consisting of kinases and phosphatases.
44. The method of paragraph 1, where the enzyme is selected from the group consisting of cyclases and phosphodiesterases.
45. The method of paragraph 1, where the enzyme is selected from the group consisting of proteases and protein ligases.
46. The method of paragraph 1, where the enzyme is selected from the group consisting of prenyl transferases and prenyl detransferases.
47. The method of paragraph 1, where the enzyme is selected from the group of lipases and lipid ligases.
48. The method of paragraph 1, where the substrate includes a phosphorylated polypeptide or a nonphosphorylated polypeptide.
49. The method of paragraph 1, where the substrate includes a cyclized nucleotide or a noncyclized nucleotide.
50. The method of paragraph 1, further comprising:
contacting the substrate and enzyme with a candidate compound; and
determining the ability of the candidate compound to enhance or inhibit enzyme activity by its effects on the response.
51. The method of paragraph 2, where the polypeptide includes fewer than about 50 amino acids.
52. The method of paragraph 2, where the binding partner binds to the phosphorylated polypeptide.
53. The method of paragraph 2, where the binding partner is not a polypeptide.
54. The method of paragraph 2, where the enzyme catalyzes addition or cleavage of a phosphate group to or from a protein, further comprising contacting the polypeptide with the enzyme prior to the steps of contacting, measuring, and correlating.
55. The method of paragraph 2, where the enzyme is a kinase.
56. The method of paragraph 2, where the enzyme is a phosphatase.
57. The method of paragraph 2, further comprising:
contacting the polypeptide and enzyme with a candidate compound; and
determining the ability of the candidate compound to enhance or inhibit phosphorylation or dephosphorylation of the polypeptide by its effects on the response.
58. The method of paragraph 3, where the nucleotide includes an adenine or a guanine.
59. The method of paragraph 3, where the binding partner binds to the cyclized nucleotide.
60. The method of paragraph 3, where the binding partner is not a polypeptide.
61. The method of paragraph 3, where the enzyme catalyzes cyclization or decyclization of a nucleotide, further comprising contacting the nucleotide with the enzyme.
62. The method of paragraph 3, where the enzyme is a phosphodiesterase.
63. The method of paragraph 3, where the enzyme is a cyclase.
64. The method of paragraph 3, further comprising:
contacting the nucleotide and enzyme with a candidate compound; and
determining the ability of the candidate compound to enhance or inhibit cyclization or decyclization of the nucleotide by its effects on the response.
65. The method of paragraph 3, where the step of detecting a response is performed without separating the bound nucleotide from the unbound nucleotide.
66. The method of paragraph 3, further comprising washing the sample to remove any nucleotide not bound to the binding partner prior to the step of measuring the detectable luminescence response.
67. The method of one of paragraphs 1, 2, or 3 further comprising:
providing a sample holder having a plurality of sample sites supporting a corresponding plurality of samples; and
repeating the steps of contacting, detecting, and correlating for the plurality of samples.
68. A method of detecting a phosphorylated form or a nonphosphorylated form of a polypeptide in a sample, comprising: (A) contacting the sample with a binding partner that binds specifically to one of the phosphorylated and nonphosphorylated forms of the polypeptide, but not both, the binding partner including a metal involved in the specific binding to the one form; and (B) exposing the sample to a condition capable of inducing luminescence from the sample; and (C) measuring a detectable luminescence response, where the detectable luminescence response is luminescence energy transfer, and the detectable luminescence response is indicative of the extent of binding between the binding partner and the one form.
69. A method of detecting a cyclized or noncyclized form of a nucleotide in a sample, comprising:
contacting the nucleotide with a binding partner that specifically binds to one of a cyclized form and a noncyclized form of the nucleotide, but not both, the binding partner including a metal required for specific binding of the binding partner to the one form of the nucleotide; and
exposing the nucleotide to a condition capable of producing luminescence, and
detecting a luminescence energy transfer produced by the step of exposing; where the luminescence energy transfer is indicative of the extent of binding between the one form of the nucleotide and the binding partner.
70. A method of detecting a cyclized or noncyclized form of a nucleotide in a sample, comprising:
contacting the nucleotide with a binding partner that specifically binds to one of a cyclized form and a noncyclized form of the nucleotide, but not both, substantially without regard to the base portion the nucleotide; and
exposing the nucleotide to a condition capable of producing luminescence, and
detecting a luminescence energy transfer produced by the step of exposing;
where the luminescence energy transfer is indicative of the extent of binding between the one form of the nucleotide and the binding partner.
71. The method of one of paragraphs 69 and 70, further comprising a step of contacting the nucleotide with at least one enzyme selected from cyclases and phosphodiesterases.
72. The method of paragraph 71, wherein the step of contacting the nucleotide with at least one enzyme is performed before the step of contacting the nucleotide with a binding partner.
73. The method of paragraph 71, further comprising (1) a step of contacting the at least one enzyme with a candidate compound; and (2) a step of determining the ability of the candidate compound to enhance or inhibit enzyme activity by its effect on the response.
74. The method of paragraph 71, further comprising a step of correlating the response with a cyclization or decyclization activity of the at least one enzyme on the nucleotide.
75. The method of one of paragraphs 69 and 70, where the step of contacting includes (1) a step of placing a metal salt in a liquid, the metal salt including the metal, and (2) a step of contacting the nucleotide with the metal salt after the step of placing.
76. The method of one of paragraphs 69 and 70, where the step of contacting includes a step of contacting the nucleotide with one or more metals selected from the group consisting of aluminum, gallium, and iron ions, and wherein the one or more metals are required for specific binding of the binding partner to the one form of the nucleotide.
77. The method of one of paragraphs 69 and 70, wherein the step of contacting includes a step of contacting the nucleotide with one or more metal ions selected from the group consisting of europium, strontium, terbium, and zirconium ions, and wherein the one or more metals are required for specific binding of the binding partner to the one form of the nucleotide.
78. The method of one of paragraphs 69 and 70, wherein the step of contacting includes a step of contacting with gallium, the gallium being required for specific binding of the binding partner to the one form of the nucleotide.
79. The method of one of paragraphs 69 and 70, wherein the steps of contacting and detecting are performed a plurality of times for a plurality of samples disposed in different wells of a microplate.
80. The method of one of paragraphs 69 and 70, further comprising a step of associating the metal with a distinct solid support that does not include the metal.
81. The method of one of paragraphs 69 and 70, wherein the step of detecting is performed after the step of contacting without separation of bound and unbound species of the nucleotide.
82. The method of one of paragraphs 69 and 70, wherein the steps of contacting and detecting are performed a plurality of times for a plurality of samples disposed in different wells of a microplate.
83. The method of one of paragraphs 69 and 70, wherein the nucleotide is luminescent.
84. A kit for performing the method of paragraph 62, comprising:
a cyclized or noncyclized form of a nucleotide; and
a binding partner that specifically binds to one of the cyclized form and the noncyclized form of the nucleotide, but not both, wherein the binding partner includes a metal required for specific binding of the binding partner to the one form of the nucleotide.
85. The kit of paragraph 84, further comprising an enzyme capable of converting the cyclized form of the nucleotide into the noncyclized form, or vice versa.
86. The kit of paragraph 84, wherein the kit includes a luminescently labeled, cyclized form of the nucleotide and a binding partner that specifically binds to the noncyclized form of the nucleotide.
87. The kit of paragraph 86, further comprising a phosphodiesterase enzyme.
88. The kit of paragraph 84, wherein the kit includes a luminescently labeled, noncyclized form of the nucleotide and a binding partner that specifically binds to the noncyclized form of the nucleotide.
89. The kit of paragraph 88, further comprising a cyclase enzyme.
90. A kit for performing the method of paragraph 70, comprising:
a cyclized or noncyclized form of a nucleotide; and
a binding partner that specifically binds to one of the cyclized form and the noncyclized form of the nucleotide, but not both, substantially without regard to the base portion the nucleotide.
91. A method of detecting the activity of a cyclase or a phosphodiesterase that operates on a nucleotide substrate to form a nucleotide product in a sample, comprising:
contacting the nucleotide substrate with a binding partner that specifically binds to the nucleotide substrate or to the nucleotide product but not to both, where the binding partner includes a metal required for binding between the binding partner and the nucleotide substrate or product;
contacting the nucleotide substrate with at least one of a cyclase and a phosphodiesterase;
detecting a response indicative of the extent of binding between the nucleotide substrate or product and the binding partner without separating bound and unbound fractions of such nucleotide substrate or product; and
correlating the response with the activity of the enzyme.
92. A method of detecting, in a sample, a first analyte or a second analyte related to the first analyte by a molecular modification, comprising:
contacting the sample with a metal that forms a covalent coordination complex with one of the first and second analytes, but not both; and
detecting a response indicative of the extent of binding between metal and the one analyte without separating bound and unbound species of the one analyte produced by the step of contacting.
93. A method of detecting, in a sample, a first analyte or a second analyte related to the first analyte by a molecular modification, comprising:
contacting the sample with gallium, the gallium specifically binding to one of the first and second analytes, but not both; and
detecting a response indicative of the extent of binding between the gallium and the one analyte.
The following claims particularly point out certain combinations and subcombinations regarded as novel and nonobvious and directed to one of the inventions. These claims may refer to “an” element or “a first” element or the equivalent thereof; such claims should be understood to include incorporation of one or more such elements, neither requiring nor excluding two or more such elements. Inventions embodied in other combinations and subcombinations of features, functions, elements, and/or properties may be claimed through amendment of the present claims or through presentation of new claims in this or a related application. Such claims, whether directed to a different invention or to the same invention, and whether broader, narrower, equal, or different in scope to the original claims, also are regarded as included within the subject matter of the inventions of the present disclosure.
Claims
1. A method of detecting the activity of an enzyme that operates on an enzyme substrate to form an enzyme product in a sample, comprising:
- contacting the substrate or product with a binding partner that specifically binds to the substrate or to the product but not to both, where the binding partner includes a metal that is involved in binding between the binding partner and the substrate or product;
- contacting the substrate with the enzyme;
- exposing the sample to light capable of inducing luminescence from the sample; and
- measuring a detectable luminescence energy transfer response, without separating the bound substrate or product from the unbound substrate or product, where the detectable luminescence energy transfer response is indicative of the extent of binding between the substrate or product and the binding partner; and
- correlating the luminescence energy transfer response with the activity of the enzyme.
2. The method of claim 1, where the metal is selected from the group consisting of aluminum, iron, gallium, europium, and terbium.
3. (canceled)
4. (canceled)
5. The method of claim 1, where measuring the detectable luminescence energy transfer response includes measuring luminescence intensity.
6. The method of claim 1, where at least one of the substrate and/or product, and the binding partner includes an energy transfer donor, and at least the other of the substrate and/or product, and the binding partner includes an energy transfer acceptor, such that luminescence energy transfer can occur between the donor and the acceptor.
7. (canceled)
8. (canceled)
9. The method of claim 6, where the binding partner includes an energy transfer donor or acceptor, and that energy transfer donor or acceptor includes the metal that is involved in binding between the binding partner and the substrate or product.
10. (canceled)
11. (canceled)
12. The method of claim 9, where the energy transfer donor comprises a lanthanide.
13. (canceled)
14. (canceled)
15. (canceled)
16. The method of claim 12, where the energy transfer donor further comprises an organic chelator and a sensitizer moiety.
17. (canceled)
18. (canceled)
19. (canceled)
20. The method of claim 12, where the lanthanide chelate has the formula
- where the lanthanide chelate is associated with the binding partner via a metal-binding functional group, and is optionally further substituted by one or more reactive functional groups or conjugated substances.
21. The method of claim 1, where the substrate is a polypeptide, and where the substrate and product are related by a posttranslational modification.
22. The method of claim 21, where the posttranslational modification is phosphorylation or dephosphorylation of the polypeptide and the enzyme is selected from the group consisting of kinases and phosphatases.
23. (canceled)
24. (canceled)
25. The method of claim 1, where the substrate is a nucleotide, the substrate and product are related by a cyclization or decyclization of the nucleotide, and the enzyme is selected from the group consisting of cyclases and phosphodiesterases.
26. The method of claim 1, where the substrate is a lipid, lipid precursor or lipid fragment, and the enzyme is selected from the group of lipases and lipid ligases.
27. The method of claim 1, where the substrate includes a phosphorylated polypeptide or a nonphosphorylated polypeptide.
28. The method of claim 1, where the substrate includes a cyckzed nucleotide or a noncyclized nucleotide.
29. The method of claim 1, further comprising:
- contacting the substrate and enzyme with a candidate compound; and
- determining the ability of the candidate compound to enhance or inhibit enzyme activity by its effects on the response.
30. The method of claim 1, further comprising:
- providing a sample holder having a plurality of sample sites supporting a corresponding plurality of samples: and
- repeating the steps of contacting, detecting, and correlating for the plurality of samples.
31. A kit for performing the method of claim 1, comprising:
- a phosphorylated or nonphosphorylated polypeptide; and
- a binding partner that specifically binds to one of the phosphorylated and nonphosphorylated polypeptide but not both, wherein the binding partner includes a metal required for specific binding of the binding partner to the one form of the polypeptide.
32. A kit for performing the method of claim 1, comprising:
- a cyclized or noncyclized form of a nucleotide; and
- a binding partner that specifically binds to one of the cyclized form and the noncyclized form of the nucleotide, but not both, wherein the binding partner includes a metal required for specific binding of the binding partner to the one form of the nucleotide.
33. A method of detecting phosphorylation or nonphosphorylation of a polypeptide in a sample, comprising:
- contacting the polypeptide with a binding partner that specifically binds to the phosphorylated polypeptide or to the nonphosphorylated polypeptide but not to both, where the binding partner includes a metal that is required for binding between the binding partner and the phosphorylated polypeptide or nonphosphorylated polypeptide:
- exposing the sample to light capable of inducing luminescence from the sample; and
- measuring a detectable luminescence energy transfer, where the luminescence energy transfer is indicative of the extent of binding between the polypeptide and the binding partner without separating the bound polypeptide from the unbound polypeptide; and
- correlating the luminescence energy transfer with the extent of phosphorylation or nonphosphorylation of the polypeptide, or with the activity of an enzyme that affects phosphorylation or nonphosphorylation of the polypeptide.
34. A method of detecting cyclization or noncyclization of a nucleotide in a sample, comprising:
- contacting a nonradioactive nucleotide with a binding partner that specifically binds to a cyclized nucleotide or to a noncyclized nucleotide but not to both, substantially without regard to the nucleoside portion of the nucleotide, where the binding partner includes a metal that is required for binding between the binding partner and the cyclized nucleotide or noncyclized nucleotide;
- exposing the sample to capable of inducing luminescence from the sample; and
- measuring a detectable luminescence energy transfer, where the luminescence energy transfer is indicative of the extent of binding between the nucleotide and the binding partner; and
- correlating the response with the extent of cyclization or noncyclization of the nucleotide, or with the activity of an enzyme that affects cyclization or noncyclization of the nucleotide.
Type: Application
Filed: Dec 14, 2009
Publication Date: Dec 2, 2010
Inventors: ANNEGRET BOGE (SAN JOSE, CA), J. RICHARD SPORTSMAN (ENCINITAS, CA), ELIZABETH GAUDET (MENLO PARK, CA), GEORGE G. YI (SUNNYVALE, CA)
Application Number: 12/637,598
International Classification: G01N 33/573 (20060101);
