To Identify An Enzyme Or Isoenzyme Patents (Class 435/7.4)
  • Patent number: 11643642
    Abstract: The present invention provides engineered penicillin G acylase (PGA) enzymes, polynucleotides encoding the enzymes, compositions comprising the enzymes, and methods of using the engineered PGA enzymes.
    Type: Grant
    Filed: May 23, 2018
    Date of Patent: May 9, 2023
    Assignee: Codexis, Inc.
    Inventors: Rama Voladri, Christopher Michael Micklitsch, Oscar Alvizo, Jovana Nazor, Da Duan, Judy Victoria Antonio Viduya, Stephan Jenne, Chihui An, Keith Allen Canada, Paul N. Devine, Iman Farasat, Anna Fryszkowska, Katrina W. Lexa, Robert Kevin Orr
  • Patent number: 11614448
    Abstract: The present invention relates to compositions and methods for the in vitro diagnosis of prostate cancer, wherein said compositions comprise an antibody binding to progastrin and said methods comprise the use of an antibody binding to progastrin.
    Type: Grant
    Filed: March 30, 2018
    Date of Patent: March 28, 2023
    Assignee: ECS-Progastrin SA
    Inventor: Alexandre Prieur
  • Patent number: 11467167
    Abstract: Provided herein are methods for developing selected reaction monitoring mass spectrometry (LC-SRM-MS) assays.
    Type: Grant
    Filed: December 4, 2019
    Date of Patent: October 11, 2022
    Assignee: Biodesix, Inc.
    Inventors: Paul E. Kearney, Xiao-Jun Li, Clive Hayward
  • Patent number: 11389405
    Abstract: Disclosed herein is a particle containing an inner liposomal vesicle (ILV) encapsulating a therapeutic agent; an outer liposomal vesicle (OLV) encapsulating the ILV; a membrane fusion-promoting agent; and a pH-altering agent. Also disclosed are methods of delivering a therapeutic agent to a subject comprising: a) providing a herein disclosed particle b) triggering ILV and OLV fusion; and c) releasing the therapeutic agent outside of the OLV. Also disclosed are methods for treating a disease in a subject in need thereof comprising: administering to a subject a herein disclosed particle. Also disclosed are methods to release insulin to an environment comprising increased glucose levels, the method comprising exposing to the environment a herein disclosed particle.
    Type: Grant
    Filed: September 17, 2018
    Date of Patent: July 19, 2022
    Assignee: North Carolina State University
    Inventors: Zhen Gu, Zhaowei Chen
  • Patent number: 11319531
    Abstract: The present invention provides engineered transglutamirase enzymes, polynucleotides encoding the enzymes, compositions comprising the enzymes, methods of producing these enzymes, and methods of using the engineered transglutaminase enzymes.
    Type: Grant
    Filed: November 2, 2018
    Date of Patent: May 3, 2022
    Assignee: Codexis, Inc.
    Inventors: Jovana Nazor, Jie Yang, Goutami Banerjee, Xiyun Zhang, James Nicholas Riggins, Erika M. Milczek, Jeffrey C. Moore
  • Patent number: 11319530
    Abstract: Provided herein are host cells or host cellular expression systems that express a membrane protein. Also, methods are provided that use such host cells or host cellular expression systems to produce higher amounts of the membrane proteins. Further, the cells or cellular systems can be used as tools for the functional characterization of membrane proteins, as well as for screening and drug discovery efforts targeting membrane proteins.
    Type: Grant
    Filed: May 11, 2018
    Date of Patent: May 3, 2022
    Assignee: President and Fellows of Harvard College
    Inventors: Jonathan Beckwith, Dana Boyd, Jessica Blazyk, Feras Hatahet
  • Patent number: 11313831
    Abstract: An interdigitated electrode biosensor includes an insulating layer configured to fully cover a sensor forming region of a substrate, a first interdigitated microelectrode configured such that a plurality of first protruding electrodes is arranged in a shape of a comb on the substrate, a second interdigitated microelectrode configured such that a plurality of second protruding electrodes is arranged in a shape of a comb and each interdigitates with the plurality of first protruding electrodes, and a plurality of receptors that is immobilized in a space between the first interdigitated microelectrode and the second interdigitated microelectrode and reacts specifically to target biomaterials.
    Type: Grant
    Filed: November 9, 2016
    Date of Patent: April 26, 2022
    Assignee: Korea Institute of Science and Technology
    Inventors: Kyoseon Hwang, Jinsik Kim, Hye Jin Kim, Yong Kyoung Yoo, Youngsoo Kim
  • Patent number: 11268120
    Abstract: According to one aspect, the present disclosure provides a method of identifying a substrate of a transglutaminase using a peptide array comprising a plurality of peptides. The method includes the steps of contacting the peptides in the peptide array with the transglutaminase, allowing the transglutaminase to bind to the peptides, and identifying the substrate of the transglutaminase.
    Type: Grant
    Filed: June 16, 2017
    Date of Patent: March 8, 2022
    Assignee: Roche Sequencing Solutions, Inc.
    Inventors: Thomas Albert, Frank Bergmann, Victor Lyamichev, Jigar Patel, Michael Schraeml, Wojtek Steffen
  • Patent number: 11213595
    Abstract: The present invention relates to an antibody-fluorescent dye conjugate capable of cancer cell-specific fluorescence imaging diagnosis. The fluorescent dye comprises a covalently labeled antibody and is quenched by interaction with an amino acid residue such as tryptophan, tyrosine, histidine, and methionine in the antibody and upon binding of the antibody to an antigen present on a cell surface to emit fluorescence, whereby cells having a target antigen thereon can be imaged for diagnosis. When using the antibody-fluorescent dye conjugate according to the present invention during in vitro cell assays, high-throughput screening of cells, and cytodiagnosis based on microfluidics, the presence of cancer cells having a specific antigen expressed thereon can be detected at high specificity and sensitivity without a washing process, and the position of primary and metastatic cancer cells can be detected at high contrast within a short time.
    Type: Grant
    Filed: September 21, 2018
    Date of Patent: January 4, 2022
    Assignee: National Cancer Center
    Inventors: Yong-Doo Choi, Hyun-Jin Kim
  • Patent number: 11175295
    Abstract: Disclosed herein are antibodies having binding specificity to the amino acid sequences Ala Ser Ser Gly Leu Thr Val Glu Val Asp (SEQ ID NO:1) and Thr Val Glu Val Asp (SEQ ID NO:14), and methods of detecting cell death in a sample, comprising contacting the sample with a first antibody specific for a C-terminal amino acid sequence Ala Ser Ser Gly Leu Thr Val Glu Val Asp (SEQ ID NO:1) or Thr Val Glu Val Asp (SEQ ID NO:14) of a CK18 protein fragment having a C-terminal amino acid sequence of Val Glu Val Asp (SEQ ID NO:2) and a second antibody that specifically binds an epitope that is present in both full-length CK18 and the CK18 protein fragment, and that does not overlap with SEQ ID NO:1 or SEQ ID NO:14, under conditions such that the CK18 protein fragment present in the sample specifically binds to the first antibody and the second antibody, wherein one of the antibodies is bound to a solid support and the other antibody is bound to a detection moiety capable of producing a signal; optionally removing any unbo
    Type: Grant
    Filed: July 17, 2019
    Date of Patent: November 16, 2021
    Assignee: Defined Diagnostics, LLC
    Inventors: Jeffrey Allan Miller, Eddie Phillip Jeffries
  • Patent number: 11175228
    Abstract: Provided herein are sulfo n-hydroxysuccimidyl ester (sulfo-SE) linked peptides, methods of synthesis thereof, and methods of using such peptides for labeling of biomolecules. In particular, peptides comprising non-alkyl group such as serine, threonine, cysteine, tyrosine, glutamic acid, and aspartic acid are stably modified (e.g., without autoreactivity) with a sulfo-SE group and used to label or otherwise modify biomolecules.
    Type: Grant
    Filed: November 27, 2019
    Date of Patent: November 16, 2021
    Inventors: Hui Wang, Nidhi Nath, Rod Flemming, Becky Godat, Wenhui Zhou, Virginia Kincaid, Melanie Dart
  • Patent number: 11169145
    Abstract: Among the various aspects of the present disclosure is the provision of a method of detection, treatment, and monitoring of cardiovascular disease or a foot wound by detection of a novel biomarker, Fatty Acid Synthase (FAS). Briefly, therefore, the present disclosure is directed to methods that allow for improved, noninvasive, and reliable diagnosis of these conditions, particularly in subjects suffering from Type 2 Diabetes (T2D).
    Type: Grant
    Filed: May 1, 2018
    Date of Patent: November 9, 2021
    Assignee: Washington University
    Inventors: Mohamed Zayed, Clay Semenkovich
  • Patent number: 11156609
    Abstract: The invention relates to ex-situ biosensors that impedimetrically detect cardiac biomarkers of interest in a bodily fluid sample derived from a patient. The biosensors include a multi-array of vertically aligned platinum wires having immobilized thereon an aptamer that is selected to specifically and selectively bind to the cardiac markers of interest. The biosensors are contacted with a portion of the bodily fluid sample, and the aptamer binds to the cardiac markers of interest in the bodily fluid sample. As a result, an electrochemical impedance signal is generated and therefore, a change in electrochemical impedance is indicative of the presence of the cardiac markers of interest in the bodily fluid sample. The biosensors are point-of-care, on-demand devices that can be used in a medical environment, as well as in domestic settings.
    Type: Grant
    Filed: October 4, 2016
    Date of Patent: October 26, 2021
    Inventors: Prashant N. Kumta, Mitali S. Patil
  • Patent number: 11120982
    Abstract: A monitoring method based on the detection of human ingested substances, comprises: acquiring the mass spectrum in the terminal of each matrix assisted laser ionization analysis time-of-flight mass spectrometer, and storing each mass spectrum and its corresponding identity information, sampling time and residence information when sampling in the database; obtaining the mass spectrum characteristic ion peaks of known substances and/or unknown substances in each mass spectrum; and counting the frequency of the mass spectrum characteristic ion peak of the known substance and/or the unknown substance, and early warning of the mass spectrum characteristic ion peak whose frequency is greater than the first threshold.
    Type: Grant
    Filed: March 16, 2020
    Date of Patent: September 14, 2021
    Inventors: Zhiyin Zeng, Kongtao Zhu, Hui Wu, Jiansheng Zeng, Henglin Luo, Mingyi Hong
  • Patent number: 11104949
    Abstract: The present invention pertains to novel analgesics useful for treating mechanical pain. The invention suggests the use of inhibitors of ?-tubulin acetylation for inhibition of neurological sensations that are mediated by sensory neurons. The perception of mechanical pain is can be modulated by altering the ?-tubulin acetylation, in context of the invention in particular by modulation of the expression and/or activity of the enzyme ?-tubulin acetyltransferase (Atat). The invention provides the medical application of ?-tubulin acetyltransferase inhibitors as analgesics and a screening method for the identification of compounds useful in the treatment of pain.
    Type: Grant
    Filed: December 28, 2016
    Date of Patent: August 31, 2021
    Assignee: European Molecular Biology Laboratory
    Inventors: Paul Heppenstall, Shane Morley
  • Patent number: 11085935
    Abstract: The present disclosure provides methods to quantify tau phosphorylation at specific amino acid residues to predict time to onset of mild cognitive impairment due to Alzheimer's disease, stage Alzheimer's disease, guide treatment decisions, select subjects for clinical trials, and evaluate the clinical efficacy of certain therapeutic interventions.
    Type: Grant
    Filed: September 9, 2020
    Date of Patent: August 10, 2021
    Assignee: Washington University
    Inventors: Nicolas Barthelemy, Randall Bateman, Eric McDade
  • Patent number: 11012774
    Abstract: A method for producing a target directivity function that includes a set of spatially biased HRTFs. A set of left ear and right ear head related transfer functions (HRTFs) are selected. The left ear and right ear head HRTFs are multiplied with an on-camera emphasis function (OCE), to produce the spatially biased HRTFs. The OCE may be designed to shape the sound profile of the HRTFs to provide emphasis in a desired location or direction that is a function of the specific orientation of the device as it is being used to make a video recording. Other aspects are also described and claimed.
    Type: Grant
    Filed: September 18, 2019
    Date of Patent: May 18, 2021
    Assignee: APPLE INC.
    Inventors: Jonathan D. Sheaffer, Joshua D. Atkins, Peter A. Raffensperger, Symeon Delikaris Manias
  • Patent number: 10881761
    Abstract: Disclosed herein is a method of producing collagen particles. Each of the collagen particle is characterized in having a particle size of about 10-250 ?m, in which the integrity of collagen fibers therein is relatively intact.
    Type: Grant
    Filed: August 11, 2016
    Date of Patent: January 5, 2021
    Inventors: Jun-Jie Wang, Pei-Hua Tsai, Kai-Chi Ku, Dar-Jen Hsieh
  • Patent number: 10866236
    Abstract: The present invention relates to detecting cleavage activity of an enzyme. The various aspects of the invention include an enzyme detection device, kit, method and use for detecting or measuring the presence in a test sample of the activity of an enzyme capable of cleaving a substrate. The invention also relates to indicator and binding molecules useful for carrying out the invention. The enzyme substrate contains a hidden binding site which is only revealed upon cleavage by the enzyme.
    Type: Grant
    Filed: October 23, 2014
    Date of Patent: December 15, 2020
    Assignee: Mologic Limited
    Inventors: Paul Davis, Gita Parekh, James Schouten
  • Patent number: 10830771
    Abstract: Disclosed is a means of enabling the construction of a more accurate PIVKA-II immunoassay system using monoclonal antibodies against human prothrombin. The PIVKA-II assay method according to the present invention comprises measuring PIVKA-II in a sample by an immunoassay using an anti-PIVKA-II antibody or an antigen-binding fragment thereof which specifically binds to PIVKA-II and a mixture of a first anti-prothrombin antibody or an antigen-binding fragment thereof which recognizes hydrophilic PIVKA-II molecules and a second anti-prothrombin antibody or an antigen-binding fragment thereof which recognizes hydrophobic PIVKA-II molecules.
    Type: Grant
    Filed: October 6, 2016
    Date of Patent: November 10, 2020
    Inventors: Yoshiyuki Kitamura, Katsumi Aoyagi
  • Patent number: 10788501
    Abstract: The instant invention relates to a qualitative predictive method, to a method, use and kit applied to the early differential diagnosis of the most prevalent forms of bacterial and viral meningitis, enabling to detect and distinguish the different forms of meningitis. The invention uses a qualitative predictive method based on combined detection and sequential analysis of the presence/absence of at least three out of four specific biomarkers.
    Type: Grant
    Filed: February 21, 2019
    Date of Patent: September 29, 2020
    Inventors: Roney Santos Coimbra, Rosiane A da Silva Pereira, Guilherme Correa De Oliveira, Ana Paula Cordeiro
  • Patent number: 10677809
    Abstract: A method and cartridge for determining an amount of at least two analytes in a biological sample and an automatic analyzer are disclosed. The cartridge may comprise a cartridge inlet, a sample holding chamber fluidically connected to the inlet, and two or more metering chambers. Each metering chamber may comprise a sample inlet, a sample outlet, and a metered outlet for dispensing a predetermined volume. At least one sample distribution channel is connected between the sample outlet of a metering chamber with a sample inlet of another metering chamber. For each metering chamber, a connecting tube fluidically connects the sample inlet with the sample holding chamber, a microfluidic structure for processing the sample into a processed sample connects to the sample outlet, and a measurement structure fluidically connects to the microfluidic structure and enables measurement of the processed sample to determine the amount of the analyte in the processed sample.
    Type: Grant
    Filed: November 9, 2017
    Date of Patent: June 9, 2020
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Christoph Boehm, Sascha Lutz, Thomas Keller
  • Patent number: 10654933
    Abstract: The present inventors discovered that additional aggregation of low-pI antibody can be suppressed by removing formed antibody aggregates after a certain period of time following Protein A column purification, acidic treatment, and neutralization. Furthermore, the present inventors found that efficient impurities removal for a low-pI antibody can be accomplished by using an anion exchange resin in the Bind/Elute mode and then a hydrophobic interaction chromatography or multimodal chromatography resin, compared with conventional methods.
    Type: Grant
    Filed: December 26, 2014
    Date of Patent: May 19, 2020
    Assignee: Chugai Seiyaku Kabushiki Kaisha
    Inventors: Yasufumi Ueda, Shohei Kobayashi, Satoko Yanagita, Takuo Kawase, Masahiro Fukunaga
  • Patent number: 10605718
    Abstract: An arrangement for the individualized in-vitro patient blood analysis includes a holography module, a Raman spectroscopy module, a biomarker module and a flow controller which are connected in a data and information transmitting manner to a central control and computer unit, which has an information transmitting connection to a database, wherein the modules are fluidically connected to a common blood sample supply and supplies for fluid other than blood via the flow controller.
    Type: Grant
    Filed: August 15, 2016
    Date of Patent: March 31, 2020
    Assignees: Leibniz-Institut Photonische Technologien E.V., Universitaetsklinikum Jena
    Inventors: Thomas Henkel, Michael Bauer, Ute Neugebauer, Juergen Popp
  • Patent number: 10585101
    Abstract: Disclosed are methods to induce dislodgement of target prostatic cells from the prostate organ, collecting said cells, and subsequently examining the cell population. Such methods comprise the administration of an agent that facilitates the dislodgement of the target cells from within the prostate, which then migrate into the urethra. Exemplary agents include 5 alpha-reductase inhibitors. The cells induced to pass into the urethra are then collected non-invasively, such as through urine or semen samples. Such collection is further strategically calculated relative the administration of the agent so as to maximize the sample collection of the target cells of interest.
    Type: Grant
    Filed: March 10, 2016
    Date of Patent: March 10, 2020
    Assignee: WAVESENSE, INC.
    Inventor: Christopher Feistel
  • Patent number: 10488410
    Abstract: A method of screening a sample of body fluid obtained from an animal subject for analyte autoantibodies reactive with one or more antigenic molecules selected from pancreatic islet cell antigenic molecules (GAD, 1A2) and insulin, or one or more variants, analogs, derivatives or fragments thereof, and a kit for use in such a method. After addition of the sample one or more complexes comprising [antigenic molecule of first source]-[analyte auto antibody]-[antigenic molecule of second source]. The antigenic molecule of first source is immobilized to a solid phase, the second is labelled.
    Type: Grant
    Filed: August 19, 2016
    Date of Patent: November 26, 2019
    Assignee: RSR LIMITED
    Inventors: Bernard Rees Smith, Jadwiga Furmaniak, Michael Powell
  • Patent number: 10481156
    Abstract: A method of screening a sample of body fluid obtained from an animal subject for analyte autoantibodies reactive with one or more antigenic molecules selected from pancreatic islet cell antigenic molecules (GAD, 1A2) and insulin, or one or more variants, analogs, derivatives or fragments thereof, and a kit for use in such a method. After addition of the sample one or more complexes comprising [antigenic molecule of first source]-[analyte auto antibody]-[antigenic molecule of second source]. The antigenic molecule of first source is immobilized to a solid phase, the second is labeled.
    Type: Grant
    Filed: August 19, 2016
    Date of Patent: November 19, 2019
    Assignee: RSR LIMITED
    Inventors: Bernard Rees Smith, Jadwiga Furmaniak, Michael Powell
  • Patent number: 10280199
    Abstract: Disclosed herein are embodiments of a method for collecting, extracting or eluting proteins and antigens from cells infected with coronavirus. The coronavirus may be a porcine coronavirus, such as porcine epidemic diarrhea virus (PEDV) or porcine delta coronavirus (PDCoV). Also disclosed are embodiments of a composition comprising the coronavirus proteins and antigens, and embodiments of a method of using such a composition. Applications for the composition include, but are not limited to, use in the preparation of antibodies against the proteins and antigens, use as reference markers for coronavirus proteins, and/or use in an immunogenic composition, such as in a vaccine composition.
    Type: Grant
    Filed: August 4, 2016
    Date of Patent: May 7, 2019
    Assignee: Phibro Animal Health Corporation
    Inventor: Byoung-Kwan Kim
  • Patent number: 10281480
    Abstract: The present invention is in the field of coagulation diagnostics and relates to methods for detecting modulators of GPIb-thrombin interaction in a sample. To this end, the sample is contacted with isolated mutated GPIb? protein and thrombin, and the formation of a complex between mutated GPIb? protein and thrombin is determined.
    Type: Grant
    Filed: March 10, 2015
    Date of Patent: May 7, 2019
    Assignee: Siemens Healthcare Diagnostics Products GmbH
    Inventor: Juergen Patzke
  • Patent number: 10214758
    Abstract: A method of digesting a lignocellulosic material is disclosed. In one embodiment, the method comprises the step of exposing the material to an effective amount of Streptomyces sp. ActE secretome such that at least partial lignocellulosic digestion occurs.
    Type: Grant
    Filed: December 10, 2012
    Date of Patent: February 26, 2019
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Brian Grant Fox, Taichi Takasuka, Adam Joel Book, Cameron Robert Currie
  • Patent number: 10202466
    Abstract: Biosensors, compositions comprising biosensors, methods of producing biosensors, and methods of using biosensors are disclosed. The biosensors comprise a fluorogen-activating peptide and a blocking peptide. The fluorogen-activating peptide and blocking peptide are covalently linked through a peptide linker. The blocking peptide associates with the fluorogen-activating peptide thereby blocking an active domain of the fluorogen-activating peptide when the linker is in an unmodified state. The peptide linker may contain an amino acid sequence that is specifically recognized as a modification substrate by a cognate enzyme. The fluorogen-activating peptide and the blocking peptide at least partially disassociate when the linker is modified by an enzyme, thereby allowing the fluorogen-activating peptide to bind a cognate fluorogen and modulate a fluorescence signal.
    Type: Grant
    Filed: March 11, 2013
    Date of Patent: February 12, 2019
    Inventor: Peter B. Berget
  • Patent number: 10191066
    Abstract: The present invention describes the method for determining the risk of future major adverse cardiovascular events, which comprises detection proteolytic fragments of IGFBP-4 or IGFBP-5 (insulin-like growth factor binding protein 4 or insulin-like growth factor binding protein 5) in patients' blood. The present invention provides antibodies and immunoas-says, suitable for specific measurement of proteolytic fragments of IGFBPs. In current invention the IGFBP fragments are suggested to be utilized as blood biomarkers for the risk prediction of major adverse cardiovascular events (MACE).
    Type: Grant
    Filed: April 13, 2012
    Date of Patent: January 29, 2019
    Assignee: HYTEST LTD.
    Inventors: Alexey G. Katrukha, Alexander B. Postnikov, Tatiana I. Smolyanova, Alexey V. Kharitonov, Natalia N. Tamm
  • Patent number: 10124047
    Abstract: Methods of neutralizing circulating antibody and mitigating the risk of clinically significant adverse responses to incompatible transfusions and transplantations are described. The methods comprise the administration to the subject of dispersible antigen-lipid constructs.
    Type: Grant
    Filed: July 9, 2015
    Date of Patent: November 13, 2018
    Inventors: Stephen Micheal Henry, Caroline Ann Oliver
  • Patent number: 10107826
    Abstract: Methods and kits for reducing non-specific binding in an immunoassay for PIVKA-II in a test sample are described, in which the test sample is reacted with an anti-prothrombin antibody in the presence of one or more of the following additives: skim milk, saponin, CaCl2, MgCl2, and a sulfobetaine zwitterionic detergent.
    Type: Grant
    Filed: May 16, 2012
    Date of Patent: October 23, 2018
    Assignee: Abbott Japan Co. Ltd.
    Inventors: Toru Yoshimura, Eisaku Yoshida, Ryotaro Chiba
  • Patent number: 10005834
    Abstract: The present invention relates to the identification of certain biomarkers for use in identifying patients who have, or are likely to develop an IL-33 mediated disease or disorder and who are more likely to respond to therapy with an IL-33 antagonist. The invention also relates to methods of treatment of an IL-33-mediated disease or disorder in a patient by administering an IL-33 antagonist to the patient in need thereof and monitoring the effectiveness of therapy using the biomarkers described herein. Also provided are methods for decreasing the level of at least one biomarker in a subject suffering from an IL-33-mediated disease or disorder, and methods for treating such diseases or disorders according to the expression levels of one or more biomarkers. The methods of the present invention comprise administering to a subject in need thereof a pharmaceutical composition comprising an interleukin-33 antagonist.
    Type: Grant
    Filed: October 5, 2016
    Date of Patent: June 26, 2018
    Assignee: Regeneron Pharmaceuticals, Inc.
    Inventors: Jamie M. Orengo, Jeanne Allinne, Wen Fury, Yu Bai
  • Patent number: 9964538
    Abstract: Disclosed are sensitive methods for detecting a target substance in low concentrations. The method can include immobilizing a target substance to a solid phase; incubating the immobilized target substance with a reporter enzyme detection probe in solution under conditions for forming target: enzyme detection probe complexes; washing the solid phase to remove any unbound reporter enzyme detection probe; incubating the target: enzyme detection probe complex with a reporter enzyme detection probe substrate in substrate reaction solution to generate one or more ionizable products; and detecting one or more of the one or more ionizable products using mass spectrometry (MS).
    Type: Grant
    Filed: November 20, 2015
    Date of Patent: May 8, 2018
    Assignee: YYZ Pharmatech, Inc.
    Inventors: John G. Marshall, Angelica Kirsten Florentinus
  • Patent number: 9895693
    Abstract: Devices, systems, methods, and kits are provided for performing separation, immobilization, blotting, and/or detection of analytes from biological samples. In some embodiments, the devices are constructed from two solid substrates with surfaces in contact. The devices include a plurality of channels formed from indentations in these surfaces. The indentations can be aligned with each other across the interface between the substrates, and realigned by shifting or sliding one substrate relative to the other. In some embodiments, the devices are constructed from three layers of a solid substrate. A separation channel in the middle layer of the device is first used for analyte separation. The middle layer can then be slid relative the top and/or bottom layer, thereby aligning the separation channel with a blotting membrane. Analytes can then be transferred to the membrane using electrodes in the top and bottom layers.
    Type: Grant
    Filed: March 9, 2015
    Date of Patent: February 20, 2018
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Clayton T. McKee, William Strong
  • Patent number: 9874558
    Abstract: An object of the present invention is to provide a method of measuring the amount of a compound containing a sugar chain in a biological sample by a sandwich immunoassay method using a labeled lectin, which method is suitable for reduction of noise originating from impurities and determination of the exact amount of a target compound. The present invention provides a method of measuring the amount of a target compound containing a sugar chain in a biological sample by a sandwich immunoassay method using a labeled lectin (including cases where a target compound-capturing substance other than an antibody is used as a ligand), the method containing adding a sugar chain compound which competes (crosses) with impurities contained in the biological sample in binding with the labeled lectin.
    Type: Grant
    Filed: November 15, 2013
    Date of Patent: January 23, 2018
    Assignee: KONICA MINOLT, INC.
    Inventors: Takeshi Isoda, Tomonori Kaneko
  • Patent number: 9827564
    Abstract: Fluidic systems and methods for analysis are provided. In some embodiments, systems and methods for improved measurement of absorbance/transmission through fluidic systems are described. Specifically, in one set of embodiments, optical elements are fabricated on one side of a transparent fluidic device opposite a series of fluidic channels. The optical elements may guide incident light passing through the device such that most of the light is dispersed away from specific areas of the device, such as intervening portions between the fluidic channels. By decreasing the amount of light incident upon these intervening portions, the amount of noise in the detection signal can be decreased when using certain optical detection systems.
    Type: Grant
    Filed: June 29, 2016
    Date of Patent: November 28, 2017
    Assignee: OPKO Diagnostics, LLC
    Inventors: David Steinmiller, Vincent Linder
  • Patent number: 9827592
    Abstract: Provided is an ultrasonic probe that is capable of easily dissipating heat generated therein using porous carbon allotrope foams. The ultrasonic probe includes: a matching layer; a piezoelectric layer disposed on a bottom surface of the matching layer; and a backing layer disposed on a bottom surface of the piezoelectric layer and formed of porous carbon allotrope foams and backing materials.
    Type: Grant
    Filed: August 28, 2014
    Date of Patent: November 28, 2017
    Inventors: Won Hee Lee, Min Seon Seo, Bo Yun Kim
  • Patent number: 9791436
    Abstract: A biosensor molecule comprises: a protease amino acid sequence; at least one sensor comprising at least one sensor amino acid sequence which is responsive to at least one target molecule; and an inhibitor of the protease activity of said protease amino acid sequence; wherein the biosensor is switchable from a protease active to a protease inactive state, or from a protease inactive to a protease active state when said sensor responds to said target molecule. The biosensor protease may be a protease of a virus such as a Potyvirus or a Flavivirus wherein the inhibitor is an autoinhibitory peptide derived from the virus. The biosensor may respond to the target molecule allosterically or may be cleaved by a target protease molecule.
    Type: Grant
    Filed: September 12, 2013
    Date of Patent: October 17, 2017
    Inventors: Kirill Alexandrov, Viktor Stein, Matt Trau
  • Patent number: 9720003
    Abstract: The present invention relates to a method for measuring Troponin I in a sample comprising the steps of providing a sample, contacting the sample with a monoclonal anti-Troponin I antibody coupled to a magnetic label, contacting the sample with a polyclonal anti-Troponin I antibody coupled to a sensor surface and detecting the magnetic label on the sensor surface. The invention further relates to a device and a cartridge for measuring Troponin I in a sample.
    Type: Grant
    Filed: December 15, 2009
    Date of Patent: August 1, 2017
    Inventors: Wendy Uyen Dittmer, Toon Hendrik Evers, Peggy De Kievit, Ricky Kamps, Joost Lambert Max Vissers, Michael Franciscus Wilhelmus Cornelis Martens, David Walterus Cornelis Dekkers
  • Patent number: 9650674
    Abstract: The present invention provides a composition comprising a fluorescent nucleic acid probe that can be cleaved by enzymatic and non-enzymatic means, and methods of using the same. Advantageously, the nucleic acid probe may be used to detect and quantify nucleic acid cleavage in vitro, in situ, ex vivo, and in vivo.
    Type: Grant
    Filed: October 17, 2013
    Date of Patent: May 16, 2017
    Assignee: BIOVENTURES, LLC
    Inventors: Alexei G. Basnakian, Yevgeniy Apostolov
  • Patent number: 9638687
    Abstract: Compositions and methods for determining post-transfusion survival or toxicity of red blood cells and the suitability of red blood cell units for transfusion by measuring the levels of one or more markers in a red blood cell sample are provided.
    Type: Grant
    Filed: August 28, 2013
    Date of Patent: May 2, 2017
    Assignee: Bloodworks
    Inventor: James Charles Zimring
  • Patent number: 9612236
    Abstract: The present invention is related to a method for detection of a biological target in an affinity assay, the method comprising the steps of providing a biological sample volume containing the biological target, adding a first capturing moiety to the biological sample volume comprising the biological target, wherein the first capturing moiety is adhered to a particle, concentration of the captured biological target into an elution volume that is smaller than the biological sample volume in step a), cleavage of the first capturing moiety or the biological target from the particle and direct or indirect detection and/or quantification of the biological target in a sandwich or competitive affinity assay format, wherein the biological target is associated with at least one capturing moiety, preferably at least two capturing moieties.
    Type: Grant
    Filed: June 14, 2011
    Date of Patent: April 4, 2017
    Assignee: Koninklijke Philips N.V.
    Inventors: Gwenola Sabatte, Menno Willem Jose Prins, Toon Hendrik Evers, Wilhelmina Maria Hardeman, Joukje Garrelina Orsel
  • Patent number: 9528998
    Abstract: Methods for diagnosing rheumatoid arthritis (RA) are disclosed, using measurement of the CCL8 protein level in a test sample from a subject. Testing for CCL8 as an indicator of RA can be combined with testing for other indicators of RA, including clinical assessments, imaging or other RA markers such as Rheumatoid factor (RF). CCL8 testing can be used for discriminating RA from other diseases or conditions, evaluating the severity of RA. Related diagnostic reagents, kits, pharmaceutical compositions, and methods for identifying a candidate substance as a therapeutic agent for treating rheumatoid arthritis are also described.
    Type: Grant
    Filed: April 14, 2011
    Date of Patent: December 27, 2016
    Inventors: Toru Yoshimura, Ryotaro Chiba
  • Patent number: 9498510
    Abstract: A polypeptide represented by SEQ ID NO: 1 and a method for treatment of a tumor and another disease associated with abnormal cell proliferation are described. The method includes administering a pharmaceutically-effective amount of the polypeptide to a patient in need thereof.
    Type: Grant
    Filed: March 15, 2016
    Date of Patent: November 22, 2016
    Inventors: Junbo Hu, Xianmin Xia, Guihua Wang
  • Patent number: 9475870
    Abstract: The present invention provides a single domain specific binding molecule having the structure FW1-CDR1-FW2-HV2-FW3a-HV4-FW3b-CDR3-FW4 in which the Framework Regions FW1, FW2, FW3a, FW3b, and FW4, the Complementarity Determining Regions CDR1 and CDR3, and the Hypervariable Regions HV2, and HV4 have amino acid sequences as defined which provide a high affinity anti-human serum albumin (HSA) binding domain.
    Type: Grant
    Filed: January 25, 2016
    Date of Patent: October 25, 2016
    Assignee: The University Court of the University of Aberdeen
    Inventors: Caroline Barelle, Mischa Roland Muller, Valerie Calabro, Jack Bikker, John Steven, Lioudmila Tchistiakova, Oleg Kovalenko, Andrea Olland
  • Patent number: 9429582
    Abstract: WW-binding protein 2 (WBP2) has been demonstrated in different studies to be a tyrosine kinase substrate, to activate ER?/PR transcription and to play a role in breast cancer. However, the role of WBP2 tyrosine phosphorylation in regulating ER function and breast cancer biology is unknown. Here, we established WBP2 as a tyrosine phosphorylation target of estrogen signaling via EGFR crosstalk. Using dominant negative, constitutively active mutants, RNAi and pharmacological studies, we demonstrated that phosphorylation of WBP2 at Tyr192 and Tyr231 could be regulated by c-Src and c-Yes kinases. We further showed that abrogating WBP2 phosphorylation impaired >60% of ER? reporter activity putatively by blocking nuclear entry of WBP2 and its interaction with ER?.
    Type: Grant
    Filed: August 25, 2011
    Date of Patent: August 30, 2016
    Assignee: National University of Singapore
    Inventor: Yoon Pin Lim
  • Patent number: 9377466
    Abstract: A molecular tool for use in a method of providing a molecule that is capable of binding a target molecule based on a set of polypeptides. A polypeptide having a sequence selected from SEQ ID NOs 1-32. The polypeptide may be used in a method of screening for a ligand-polypeptide conjugate capable of binding a target molecule for the ligand. A ligand-polypeptide conjugate, useful e.g. in therapy.
    Type: Grant
    Filed: September 6, 2011
    Date of Patent: June 28, 2016
    Assignee: Modpro AB
    Inventors: Lars Baltzer, Adam Slosarczyk, Jonas Bergquist, Anders Virtanen