Method of Detection of Nucleic Acids with a Specific Sequence Composition
This invention is a novel method for detecting and localizing specific nucleic acid sequences in a sample with a high degree of sensitivity and specificity. The method and novel compositions used in the method involve the use of Probe Nucleic Acids, the production of nucleic acid binding regions and the use of nucleic acid Target Binding Assemblies to detect and localize specific Target Nucleic Acids. The detection and localization of the Target Nucleic Acid is accomplished even in the presence of nucleic acids which have similar sequences. The method provides for a high degree of amplification of the signal produced by each specific binding event. In particular, methods and compositions are presented for the detection of HIV and HPV nucleic acid in samples. These methods and compositions find use in diagnosis of disease, genetic monitoring, forensics, and analysis of nucleic acid mixtures. Some of the novel compositions used in the detection method are useful in preventing or treating pathogenic conditions.
This application is a continuation application of co-pending application Ser. No. 12/493,877, filed Jun. 29, 2009; which is a continuation application of Ser. No. 10/407,543, filed Apr. 3, 2003; which is a continuation application of Ser. No. 08/860,844; filed Jun. 9, 1997; which is a 371 application of PCT/US95/15944, filed Dec. 7, 1995; which is a continuation-in-part application of Ser. No. 08/353,476, filed Dec. 9, 1994, now U.S. Pat. No. 5,871,902, all of which are incorporated herein by reference in their entireties.
The Sequence Listing for this application is labeled “seq-list.txt”, which was created on Mar. 28, 2006, and is 105 KB. The entire contents is incorporated herein by reference in its entirety.
BACKGROUND OF THE INVENTION1. Field of the Invention
This invention provides a method and compositions for use in binding, detecting, and amplifying the detection of specific Target Nucleic Acid sequences in a sample with fidelity and accuracy, even in the presence of closely related but different nucleic acids. The binding may involve the chaperoning and assembly of specific molecules into Target Binding Assemblies which specifically bind Target Binding Regions formed by the hybridization of Probe Nucleic Acids and Target Nucleic Acid sequences. The amplifying may involve the chaperoning and/or assembly of specific molecules into Booster Binding Assemblies which specifically bind Booster Binding Regions formed by the hybridization of Booster Nucleic Acids with Probe Nucleic Acids, Target Nucleic Acids, or other Booster Nucleic Acids. A method, and compositions, involving Hairpin Nucleic Acids is also provided to enable control of the size of specifically or non-specifically elongated Booster Nucleic Acids and Booster Binding Assemblies used in the amplification. The detecting involves providing one or more detectable labels, including radioactive, light- or fluorescent-emitting, enzymatic, or other detectable or signal-generating molecules, in association with the Probe Nucleic Acid, the Target Binding Assembly, the Booster Nucleic Acid, the Booster Binding Assembly, or the Hairpin Nucleic Acid. A method is presented for isolating nucleic acid fragments from an organism which has TBA component binding sites in order to create a probe nucleic acid and a TBA which is unique for that fragment and/or organism. Therapeutic and prophylactic uses of the Target Binding Assemblies and compositions for such use are also provided.
2. Background and Description of Related Art
There are an increasing number of cases in which it is important to be able to detect nucleic acids containing a specific sequence, hereinafter named Target Nucleic Acids (TNAs), in a sample. It is desirable to be able to detect the TNAs with the smallest number of processing steps, with the simplest components and to the exclusion of other similar but different nucleic acids, hereinafter named Cousin Nucleic Acids (CNAs). It is desirable to be able to detect specific TNAs to the exclusion of any and all CNAs in the detection sample without the necessity of amplification or other post-detection processing.
There are numerous methods which use immobilized or tagged nucleic acids as probes for TNAs. However, using known methods, it is difficult to discriminate between a TNA bound to the Probe Nucleic Acid (PNA) as opposed to a CNA bound to the PNA. For example, one or more base mismatches between the PNA and a CNA can still result in a CNA-PNA hybridization which is almost indistinguishable from a TNA-PNA hybridization. Thus, hybridization alone is not an optimal indicator that a PNA has hybridized to a unique TNA.
There are many situations in which a PNA would be used to try to determine whether a TNA was present in a sample which may contain CNAs. Hybridization of the PNA to any CNA in this situation would limit the diagnostic value that the PNA might have for the detection of a TNA, absent additional verification. Furthermore, it is desirable to be able to detect and localize TNAs with low copy numbers in samples which may contain many copies of CNAs, without the necessity of creating additional copies of the TNA. It would also be desirable to be able to confirm the presence of CNAs, independent of the TNAs, without the necessity of separating the CNAs and TNAs in the sample.
Furthermore, it would be desirable to be able to amplify the signal of even a low frequency hybridization of a particular TNA-PNA. For this purpose, a method of polymerizing multiple copies of a label, hereinafter referred to as a Booster Nucleic Acid (BNA) onto the TNA-PNA would be desirable.
The instant invention provides methods and compositions for achieving the foregoing desired objectives. As revealed by the following review, the instant compositions and methods have not been reported or suggested in the art. A general and comprehensive review of the state of art of nucleic acid detection is provided in Keller, H., M. M. Manak (1989) DNA Probes, Stockton Press.
A method has been reported for detecting base pair mismatches by chemical means in order to determine whether a PNA has hybridized to a CNA rather than to a TNA. In U.S. Pat. No. 4,794,075 to Ford et al., a method for distinguishing fragments of DNA which contain single base mismatches from their perfectly paired homologs is discussed. Single stranded regions within a duplex fragment are modified with carbodiimide, which reacts with unpaired guanine (G) and thymine (T) residues in DNA. Linear duplex DNA molecules do not react, while DNA molecules with single base mismatches react quantitatively. Following reaction with carbodiimide, the DNA molecules are fractionated on high percentage polyacrylamide gels such that modified and unmodified fragments can be distinguished. Ford et al. applied this technique in order to locate and purify DNA sequence differences responsible for phenotype variation and inherited disease. Although this method is useful for following variations in genetic material, it has a large number of steps, it requires costly components, and it does not offer a direct means of determining whether a PNA has hybridized to the TNA exclusive of CNAs in the sample.
There have been some attempts to assure that at least a portion of the hybridization between the PNA and another nucleic acid is complementary. One method involves the monitoring of transcription products which are produced if the PNA hybridizes to a nucleic acid sufficiently to be transcribed from a promoter site contained in the probe. U.S. Pat. No. 5,215,899 to Dattagupta discloses how specific nucleic acid sequences are amplified through the use of a hairpin probe which, upon hybridization with and ligation to a target sequence, is capable of being transcribed. The probe comprises a single stranded self-complementary sequence which, under hybridizing conditions, forms a hairpin structure having a functional promoter region, and further comprises a single stranded probe sequence extending from the 3′ end of the hairpin sequence. Upon hybridization with a target sequence complementary to the probe sequence and ligation of the 3′ end of the hybridized target sequence to the 5′ end of the hairpin probe, the target sequence is rendered transcribable in the presence of a suitable RNA polymerase and appropriate ribonucleoside triphosphates (rNTPs). Amplification is accomplished by hybridizing the desired TNA sequence with the probe, ligating the TNA to the PNA, adding the RNA polymerase and the rNTPs to the separated hybrids, and allowing transcription to proceed until a desired amount of RNA transcription product has accumulated. That method generally and specifically involves the use of hairpin DNA formed with a single stranded unpaired end to anneal a target sequence. When the target sequence is bound, the production of RNA transcription products is enabled. Thus, the method involves the detection of secondary transcription products rather than the use of a nucleic acid binding assembly to directly immobilize and/or localize a target sequence. A CNA could easily bind to the probe, and the lack of complementarity would not necessarily interfere with the formation of a CNA-PNA hybrid which could then support the production of unwanted transcription products.
A CNA bound to the PNA might be detected if the lack of complementarity interferes with the susceptibility of the hybrid CNA-PNA pair to be cut by a restriction endonuclease. In U.S. Pat. No. 5,118,605 to Urdea and U.S. Pat. No. 4,775,619 to Urdea, novel methods for assaying a nucleic acid analyte were provided, which employ polynucleotides having oligonucleotide sequences substantially homologous to a sequence of interest in the analyte, where the presence or absence of hybridization at a predetermined stringency provides for the release of a label from a support. Various techniques are employed for binding a label to a support, whereupon cleavage of either a single or double strand, a label may be released from a support, and the release of the label can be detected as indicative of the presence of a particular polynucleotide sequence in a sample. However, this technique has the shortcoming that a CNA-PNA pair could be cut by the restriction endonuclease, even if there is a mismatch, so long as the mismatch was outside of the endonuclease recognition region. This would lead to failure of the assay to identify a CNA-PNA hybrid.
Another method uses a branched DNA probe to detect nucleic acids. U.S. Pat. No. 5,124,246 to Urdea et al. discloses linear or branched oligonucleotide multimers useful as amplifiers in biochemical assays which comprise (1) at least one first single-stranded oligonucleotide unit (PNA) that is complementary to a single-stranded oligonucleotide sequence of interest (TNA), and (2) a multiplicity of second single-stranded, oligonucleotide units that are complementary to a single-stranded labeled oligonucleotide. Although amplified sandwich nucleic acid hybridizations and immunoassays using the multimers are described, the method has the limitation that PNA-CNA hybridization could occur and would result in production of unwanted signal.
In addition to methods for identification of TNAs, methods have been disclosed for the amplification of this DNA. In U.S. Pat. No. 5,200,314 to Urdea, an analyte polynucleotide strand having an analyte sequence (TNA) is detected within a sample containing polynucleotides by contacting the analyte polynucleotide with a capture probe (PNA) under hybridizing conditions, where the capture probe has a first binding partner specific for the TNA, and a second binding sequence specific for a solid phase third binding partner. The resulting duplex is then immobilized by specific binding between the binding partners, and non-bound polynucleotides are separated from the bound species. The analyte polynucleotide is optionally displaced from the solid phase, then amplified by PCR. The PCR primers each have a polynucleotide region capable of hybridizing to a region of the analyte polynucleotide, and at least one of the primers further has an additional binding partner capable of binding a solid-phase binding partner. The amplified product is then separated from the reaction mixture by specific binding between the binding partners, and the amplified product is detected. Although it is possible to confirm (by PCR) that a particular nucleic acid has hybridized with the PNA, confirmation is expensive and involves multiple steps.
As for reports that involve the interaction of a double stranded nucleic acid and a DNA-binding protein, a method has been described whereby a sequence of immobilized DNA which contains binding sites for a single protein is used to purify that protein. U.S. Pat. No. 5,122,600 to Kawaguchi et al. discloses a DNA-immobilized microsphere comprising DNA chains having base sequences which specifically bind a particular protein, and a carrier having a particle size of not more than 50 μm and not less than 0.01 μm which does not adsorb any protein, said carrier and said DNA chains being bound to each other by a chemical bond, and a process for purifying a protein using said microsphere. As this is a purification method for a protein, it does not disclose a method of detection of a TNA nor a method whereby more than one protein is bound to a double stranded nucleic acid for the purposes of detection and localization of specific TNA sequences.
In EP 0 453 301, a method for detecting a polynucleotide target sequence in a sample was described wherein sequences in a TNA are detected by hybridizing a first and a second PNA to the TNA. Each of said first and second PNAs contained a pre-formed duplex sequence, or a duplex that is formed through chain extension, capable of binding a nucleotide sequence specific binding protein. A method for binding a nucleotide specific binding protein to a duplex formed between a TNA and a PNA only upon formation of a duplex between the PNA and TNA is neither disclosed nor suggested.
In U.S. Pat. No. 4,556,643, a method was disclosed for the non-radioactive detection of specific nucleotide sequences in a sample which involved hybridization of a probe containing DNA binding protein specific sequences. However, this disclosure neither taught nor suggested a method for binding a nucleotide specific binding protein to a duplex formed between a TNA and a PNA only upon formation of a duplex between sequences present in the PNA and sequences present in the TNA.
BRIEF SUMMARY OF THE INVENTIONDisclosed are methods by which specific Target Nucleic Acid (TNA) sequences are detected through the use of Probe Nucleic Acids (PNAs) which, upon hybridization with TNAs, are capable of binding Target Binding Assemblies (TBAs). Each TBA binds at least one specific region of the PNA-TNA hybrid pair, the Target Binding Region (TBR). The TBA is comprised of one or more molecules, one or more of which can bind to TBR sequences in a specific and sequence or conformation dependent manner. The TBA may comprise one or more piloting sequences, called “PILOTS” or “Asymmetry Sequences,” which assemble and constrain the nucleotide binding components of the TBA to specific geometries. The PILOTS act to assemble specific nucleic acid recognition units or other pilots to which specific nucleic acid recognition units are attached into the TBAs in a predetermined fashion. The TBA may also contain one or more molecules which anchor or localize the TBA. Novel TBAs having unique discriminating characteristics which surprisingly render the TBAs useful not only as diagnostic tools but also as prophylactic or therapeutic compounds, are also disclosed. Disclosed are methods and compositions for utilization of the PNAs, TBRs, TBAs, and TBA PILOTS, including their utilization as components of diagnostic and forensic test kits and the utilization of the novel TBAs as prophylactic or therapeutic agents.
The PNAs, in addition to TNA-specific sequences, may also contain one or more sequences, 1/2 BBRs, capable of hybridizing with complementary 1/2 BBRs in Booster Nucleic Acids (BNAs). Through hybridization of added BNAs to the starter 1/2 BBRs present in the PNAs, extensions of the PNAs are made in the form of PNA-BNA and then BNA-BNA hybrids. These extensions can contain one or more Booster Binding Regions (BBRs). Each BBR is capable of binding a Booster Binding Assembly (BBA). The BBA is comprised of molecules, one or more of which can bind to a BBR in a specific and sequence or conformation dependent manner. The BBA may comprise one or more piloting sequences, called “PILOTS” or “Asymmetry Sequences,” which assemble and constrain the nucleotide binding components of the TBA to specific geometries. The PILOTS act to assemble specific nucleic acid recognition units or other pilots to which specific nucleic acid recognition units are attached into the BBAs in a predetermined fashion. The BBA may contain molecules which anchor or localize the BBA or which allow for detection of the bound BBAs and thereby of the TBA-TNA-PNA complexes to which they, in turn, are bound. Disclosed are methods and compositions for utilization of the 1/2 BBRs, BNAs, BBRs, BBAs, and BBA PILOTS, including their utilization as components of diagnostic and forensic test kits.
Methods and compositions are disclosed for the use of Hairpin Nucleic Acids (HNAs) as capping structures. The HNAs contain a self-hybridizing region and a single stranded 1/2 BBR which, under hybridizing conditions, can hybridize directly to the 1/2 BBRs in the PNAs or the 1/2 BBRs in BNAs already bound to the PNAs, to terminate the extension of BNAs onto the PNA or onto other BNAs.
Methods and compositions are disclosed for test procedures and the production of a test kit containing PNAs, TBAs, TBRs, BNAs, BBRs, BBAs and HNAs for the detection, localization and differentiation of specific nucleic acid sequences, including nucleic acid sequences which are found in human cells, in the Human Immunodeficiency Virus (HIV), Human Papillomavirus (HPV), and in other nucleic acid containing systems including viruses and bacteria.
Accordingly, it is an object of this invention to provide methods and compositions for use in binding, detecting, and amplifying the detection of specific Target Nucleic Acid sequences in a sample with fidelity and accuracy, even in the presence of closely related but different nucleic acid sequences. Accordingly, it is an object of this invention to provide methods and compositions for the creation of Target Binding Assemblies which specifically bind Target Binding Regions formed by the hybridization of Probe Nucleic Acids and Target Nucleic Acid sequences.
Another object of this invention is to provide a method and compositions for the creation of Booster Binding Assemblies which specifically bind Booster Binding Regions formed by the hybridization of Booster Nucleic Acid sequences with Probe Nucleic Acids, Booster Nucleic Acids and Hairpin Nucleic Acids.
Another object of this invention is to provide a method and compositions containing Hairpin Nucleic Acids which enable the control of the size of specifically or non-specifically elongated Booster Nucleic Acids and Booster Binding Assemblies used in amplification of PNA-TNA hybridization events.
Another object of this invention is to provide a method and compositions for use in the selection, assembly and or chaperoning of specific molecules, each with nucleic acid binding discriminating capabilities, into Target and Booster Binding Assemblies.
Another object of this invention is to provide a method and compositions for use in amplifying the detection of Target Binding assemblies bound to Target Binding Regions using Booster Binding Assemblies and Booster Nucleic Acids.
Another object of this invention is to provide a method and compositions which allow the use of one or more detectable labels, including but limited to radioactive labels, light emitting, fluorescent, enzymatic or other signal generating molecules. These labels are used in association with Probe Nucleic Acids, Target Binding Assemblies, Booster Binding Assemblies, Booster Nucleic Acids or Hairpin Nucleic Acids.
Another object of this invention is to provide a method for isolating nucleic acid fragments form an organism which has TBA component binding sites in order to create Probe Nucleic Acids and TBAs which are unique for that fragment or organism.
The following illustrations are contained in
The hybrids which form the TBRs and BBRs are useful in the present invention. The PNAs and BNAs, as indicated in
SEQ ID NO. 1 corresponds to FIG. 5-Ia-1 and shows the class I MHC NF-kB binding site.
SEQ ID NO. 2 corresponds to FIG. 5(Ia) and shows the B2-microglobulin NF-kB binding site.
SEQ ID NO. 3 corresponds to FIG. 5(Ia) and shows the kappa immunoglobulin NF-kB binding site.
SEQ ID NO. 4 corresponds to FIG. 5(Ia) and shows one of the HIV NF-kB binding sites.
SEQ ID NO. 5 corresponds to FIG. 5(Ia) and shows one of the HIV NF-kB binding sites.
SEQ ID NO. 6 corresponds to FIG. 5(Ia) and shows the c-myc NF-kB binding site.
SEQ ID NO. 7 corresponds to FIG. 5(IIa) and shows a double HIV NF-kB binding site.
SEQ ID NO. 8 corresponds to FIG. 5(IIa) and shows a double HIV NF-kB binding site.
SEQ ID NOS. 9-16 correspond to FIG. 5(IIa) and show a double binding site with one site being an HIV NF-kB binding site, and the other site being an HIV SP1 binding site.
SEQ ID NOS. 17-18 correspond to FIG. 5(IIa) and show a double HIV SP1 binding site.
SEQ ID NOS. 19-31 correspond to FIG. 5(IIIa) and show a double HIV NF-kB binding site and an HIV SP1 binding site.
SEQ ID NOS. 32-33 correspond to FIG. 5(IVa) and show a quadruple binding site where two sites are HIV NF-kB binding sites and two sites are HIV SP1 binding sites.
SEQ ID NO. 34 corresponds to FIG. 5VIa) and shows a quintuple binding site where two sites are HIV NF-kB binding sites and three sites are HIV SP1 binding sites.
SEQ ID NO. 35 is an example of a 1/2 BBR, in this case the OL1, OL2 and OL3 elements of the bacteriophage lambda left operator, including intervening sequences.
SEQ ID NO. 36 is an example of a 1/2 BBR, in this case the OR3, OR2 and OR1 elements of the bacteriophage lambda right operator, including intervening sequences.
SEQ ID NO. 37 is the HIV LTR.
SEQ ID NO. 38 is a PNA complementary to PNA of the HIV LTR.
SEQ ID NO. 39 is a PNA complementary to a different PNA of the HIV LTR than SEQ ID NO. 38.
SEQ ID NO. 40 is a PNA complementary to part of the HIV LTR and it also contains a 1/2 BBR and an overhang sequence for polymerizing BNAs onto the PNA.
SEQ ID NO. 41 is a BNA complementary to the SEQ ID NO. 40 1/2 BBR.
SEQ ID NO. 42 is a BNA that will polymerize onto the SEQ ID NO. 41 BNA and which, with SEQ ID NOS. 40 and 41, creates a PstI recognition site.
SEQ ID NO. 43 is a BNA that is complementary to the SEQ ID NO. 42 BNA and which completes a BamHI recognition site.
SEQ ID NO. 44 is an RNA which has a BamHI recognition site that will hybridize with the BamHI recognition site created by SEQ ID NOS. 42 and 43 to the growing polymer.
SEQ ID NO. 45 is a second PNA which, like SEQ ID NO. 40, is complementary to part of the HIV LTR, but not to the same sequence as SEQ ID NO. 40. SEQ ID NO. 45 also encodes a 1/2 BBR and an overhang which will allow polymerization of BNAs starting with a Sph1 recognition site.
SEQ ID NOS. 46-62 are human papillomavirus (HPV) specific PNAs which, upon hybridization with HPV sequences, form TBRs which bind HPV DNA binding proteins.
SEQ ID NOS. 63-71 are NF-kB DNA recognition units for incorporation into TBAs.
SEQ ID NO. 72 is a nuclear localization sequence.
SEQ ID NO. 73 is a SP1 sequence recognition unit.
SEQ ID NO. 74 is a TATA binding protein recognition unit.
SEQ ID NOS. 75-84 are papillomavirus E2 DNA recognition units.
SEQ ID NOS. 85-92 are asymmetry sequences.
SEQ ID NO. 93 is an arabidopsis TATA binding protein recognition unit.
SEQ ID NO. 94 is an HPV-16-E2-1 DNA binding protein recognition unit.
SEQ ID NO. 95 is an HPV-16-E2-2 DNA binding protein recognition unit.
SEQ ID NO. 96 is an HPV-18-E2 DNA binding protein recognition unit.
SEQ ID NO. 97 is an HPV-33-E2 DNA binding protein recognition unit.
SEQ ID NO. 98 is a bovine papillomavirus E2 DNA binding protein recognition unit.
SEQ ID NOS. 99-102 are exemplary linker sequences.
SEQ ID NO. 103 is an exemplary nuclear localization signal sequence (NLS).
SEQ ID NOS. 104-108 are exemplary chaperone sequences.
SEQ ID NOS. 109-116 are exemplary assembled TBA sequences.
SEQ ID NO. 117 is a consensus NF-kB binding site.
SEQ ID NO. 118 an HIV Tat amino acid sequence.
Abbreviations
It should also be understood from the disclosure which follows that when mention is made of such terms as target binding assemblies (TBAs), booster binding assemblies (BBAs), DNA binding proteins, nucleic acid binding proteins or RNA binding proteins, what is intended are compositions comprised of molecules which bind to DNA or RNA target nucleic acid sequences (TNAs) irrespective of the specificity of the category of binding molecules from which they are derived. Thus, for example, a TBA adapted to bind to human immunodeficiency virus sequences may be most similar to an NF-KB transcriptional factor which typically binds DNA sequences. However, as used herein, it will be understood that the TBA may be adapted for optimal use to bind to RNA sequences of a particular sequence composition or conformation.
The fidelity of the detection method disclosed herein depends in large measure on the selective binding of TBAs and BBAs to particular nucleic acid motifs. It should be understood throughout this disclosure that the basis of TBA and BBA discrimination of TNAs from related sequences (cousin nucleic acids or CNAs) may be the formation of precise probe nucleic acid (PNA)-target nucleic acid (TNA) hybrid segments (PNA-TNA hybrids). However, the basis of discrimination may just as well be the formation of a particular conformation, and may not require the complete absence of mismatched-base pairing in the TNA-PNA hybrid. Accordingly, the basis of TBA or BBA operation should be understood throughout to depend on discrimination of any property unique to the TNA-PNA hybrid as opposed to any properties displayed by any PNA-CNA hybrids that may be formed in a test sample contacted with a given PNA.
DETAILED DISCLOSURE OF THE INVENTIONThe present invention provides a method for specifically identifying a target nucleic acid (TNA) in a sample through the use of target binding assemblies (TBAs) which incorporate specific nucleic acid binding proteins. By using probe nucleic acids (PNAs) specific to a given TNA sequence, and a TBA which is specific to the duplex target binding region (TBR) formed upon formation of hybrid TNA-PNA sequences, a stable TBA-TNA-PNA complex is formed. By additionally providing specific amplifiable sequences in the PNA, in addition to sequences which specifically contribute to the formation of the TBR recognized by the TBA, the binding of the PNA to the TNA is detected and the detection amplified. For this purpose, any of a number of nucleic acid amplification systems, including polymerase chain reaction, or the use of branched DNA, each branch of which contains a detectable label, may be used. In particular, a novel method of amplification is described herein where the amplifiable portion of the PNA contains sequences onto which booster nucleic acids (BNAs) may be polymerized. Upon formation of each BNA-PNA hybrid, a booster binding region (BBR) is formed to which a booster binding assembly (BBA) binds specifically. If detectably labeled, the BBAs or BNAs provide essentially unlimited amplification of the original TNA-PNA binding event.
According to this invention, the TNA will be understood to include specific nucleic acid sequences. The TBA will be understood to be any molecular assembly which can specifically and tightly bind to a formed TNA-PNA hybrid. The TBA will contain one or more molecules whose sequences are sufficient to bind to the TBR. Nucleic acid binding domains which are known can either be used directly as components of the TBA or modified according to the teachings provided herein. The most readily available molecules with such sequences are the DNA-binding domains of DNA-binding proteins. Specifically, many DNA or RNA binding proteins are known which can either be used directly as the known, unmodified protein, or the TBA may be a nucleic acid binding protein, modified according to the specific teachings provided herein. In the latter case, specific modifications that are desirable would include optimization of binding affinities, removal of unwanted activities (such as nuclease activity and reorganization of the TBA in the presence of other molecules with an affinity for components of the TBA), optimization of selectivity of a target sequence over closely related sequences, and optimization of stability.
Examples of DNA binding proteins which could be used according to this invention are the DNA-binding portions of the transcription factor NF-kB (p50 and p65), NF-IL6, NF-AT, rel, TBP, the papilloma virus' E2 protein, sp1, the repressors cro and CI from bacteriophage lambda, and like proteins are well known proteins whose DNA binding portion has been isolated, cloned, sequenced, and characterized. In addition, any other DNA-binding protein or portion of a protein that is necessary and sufficient to bind to a TBR hybrid or a BBR is included. This includes proteins or portions of wild-type proteins with altered DNA binding activity as well as protein created with altered DNA-binding specificity, such as the exchange of a DNA-binding recognition helix from one protein to another. In addition, proteins which exhibit nucleic acid binding and other nucleic acid functions, such as restriction endonucleases, could be used as the nucleic acid binding function. Proteins which bind to target regions in DNA-RNA hybrids as well as RNA-RNA hybrids are included. (See, for example, Shi 1995, DeStefano 1993, Zhu 1995, Gonzales 1994, Salazar 1993, Jaishree 1993, Wang 1992, Roberts 1992, Kainz 1992, Salazar 1993(b)). The binding assemblies may be constructed with the use of a molecule which chaperones portions of the binding assembly so that specific component combinations and geometries can be achieved. This molecule is designated here as a PILOT. Pilots can be comprised of proteins or any combination of organic and inorganic materials which achieve the combinatorial selection and/or to induce specific geometries between members of the TBA or BBAs. A chaperone is a stable scaffold upon which a TBA or BBA may be constructed such that the correct conformation of the TBA or BBA is provided while at the same time eliminating undesirable properties of a naturally occurring nucleic acid binding protein. As a specific example of this embodiment, a modified version of the pleiotropic transcription factor, NF-kB, is provided using a modified bacteriophage lambda cro protein as the chaperone. Each NF-kB binding dimer retains the picomolar binding affinity for the NF-kB binding site while at the same time the binding assembly presents several advantageous manufacturing, stability, and specificity characteristics.
In view of the foregoing, the various aspects and embodiments of this invention are described below in detail.
1. The Probe Nucleic Acids (PNAs) and their preparation. The PNAs of the present invention comprise at least three principal parts joined together. With reference to
The PNA of the present invention can be prepared by any suitable method. Such methods, in general, will include oligonucleotide synthesis and cloning in a replicable vector. Methods for nucleic acid synthesis are well-known in the art. When cloned or synthesized, strand purification and separation may be necessary to use the product as a pure PNA. Methods of preparing RNA probes are well known (see for example Blais 1993, Blais 1994, which uses in vitro transcription from a PCR reaction incorporating a T7 RNA polymerase promoter).
The length and specific sequence of the PNA will be understood by those skilled in the art to depend on the length and sequence to be detected in a TNA, and the strictures for achieving tight and specific binding of the particular TBA to be used (see discussion on TBAs below). In general, PNAs of sequence lengths between about 10 and about 300 nucleotides in length are adequate, with lengths of about 15-100 nucleotides being desirable for many of the embodiments specifically exemplified herein.
It should also be understood that the PNA may be constructed so as to contain more than one 1/2 TBR and to produce more than one TBR for one or more TBAs, same or different, as well as complex TBRs recognized by novel duplex and multiplex TBAs (see description below regarding these novel TBAs) upon hybridization of the PNAs and TNAs.
As shown in
As shown in
In a further aspect of the embodiment shown in
In order to more clearly exemplify this aspect of the invention, it is necessary to emphasize that the TBR may have a helical structure. Thus, while PNA1 creates TBRs on one “face” of the helix, PNA2 creates a TBR on either the same or a different face of the helix, depending on the distance between the middle of each TBR (underlined in
In terms of a diagnostic or forensic kit for HIV, those skilled in the art would understand that the aforementioned aspects of this invention allow for the tailoring of the components of the diagnostic or forensic kit to match what is known at any given time about the prevalent strains of HIV or another pathogen or disease condition. It will also be appreciated by those skilled in the art that, while detection of HIV infection is not the only utility of the instant invention, due to the mutability of the HIV genome, it is probably one of the most complex test environments for such a diagnostic. It is precisely in such a mutable environment, however, where the flexibility of the instant method, coupled with its ability to discriminate between very closely related sequences, may be most clearly appreciated. In less mutable environments, some of the sophistication to which this invention is amenable need not be utilized. Thus, in a diagnostic kit for papillomavirus infection, all of the discrimination characteristics of the TBA-TBR interaction are available, along with the ability to amplify the signal using the BNAs and BBAs, but a single, simple PNA, such as any one of SEQ ID NOS. 46-62, may be used which identifies unique papillomavirus sequences, which also are known to bind to a TBA such as the papillomavirus E2 protein or truncated DNA binding portions thereof (see Hegde et al. [1992] Nature 359:505-512; Monini et al. [1991] J. Virol. 65:2124-2130).
In applying the instant method to the detection of a particular TNA for the purposes of assessing whether certain nucleic acids are present which are associated with the progression of melanoma, hepatoma, breast, cervical, lung, colon, prostate, pancreatic or ovarian cancers, the TNA may be obtained from biopsy materials taken from organs and fluids suspected of containing the cancerous cells. For the detection of genetic deficiencies, the TNA may be obtained from patient samples containing the affected cells. For detection of fermentation contaminants and products in the manufacture of food, chemical or biotechnology products or in the bioremediation of wastes, the TNA may be obtained from samples taken at various stages in the fermentation or treatment process. For detection of food or drug pathogens or contaminants, the TNA sample may be obtained from the food or drug, swabs of food or surfaces in contact with the food, fluids in contact with the food, processing materials, fluids and the like associated with the manufacture of or in contact with the food, drug, or biological samples taken from those in contact with the food or drug or the like.
2. The Booster Nucleic Acids (BNAs), Booster Binding Regions (BBRs) and their preparation. The BNAs of the present invention are comprised of at least one or more 1/2 BBRs coupled to an OSA. The 1/2 BBRs can hybridize to complementary 1/2 BBRs contained in the PNA, other BNAs or an HNA.
With reference to
With reference to
Under hybridizing conditions, the BNA illustrated in FIG. 3(II), when combined with the PNA illustrated in
Under hybridizing conditions, the BNA-BNA hybrid illustrated in FIG. 3(IVb), when combined with the PNA illustrated in FIG. 3(Vb), creates the PNA-BNA hybrid illustrated in FIG. 3(VI) containing a BBR, two additional. BNA-BNA hybrids containing BBRs, and an unhybridized extension with an additional 1/2 BBR sequence, a “booster” sequence. The BBRs created by said hybridization can be identical, similar or dissimilar in sequence. The BBRs created by said hybridization can bind identical, similar or dissimilar BBAs (see below). The BNAs may be prepared in a fashion analogous to preparation of the PNAs.
3. The Target Nucleic Acids (TNAs) and their preparation. The first step in detecting and amplifying signals produced through detection of a particular TNA according to the present method is the hybridization of such target with the PNA in a suitable mixture. Such hybridization is achieved under suitable conditions well known in the art.
The sample suspected or known to contain the intended TNA may be obtained from a variety of sources. It can be a biological sample, a food or agricultural sample, an environmental sample and so forth. In applying the instant method to the detection of a particular TNA for the purposes of medical diagnostics or forensics, the TNA may be obtained from a biopsy sample, a body fluid or exudate such as urine, blood, milk, cerebrospinal fluid, sputum, saliva, stool, lung aspirates, throat or genital swabs and the like. In addition, detection may be in situ (see for example Embretson 1993; Patterson 1993; Adams 1994).
Accordingly, PNAs specific to vertebrates (including mammals and including humans) or to any or all of the following microorganisms of interest may be envisioned and used according to the instant method:
- Corynebacteria
Corynebacterium diphtheria
- Bacillus
Bacillus thuringiensis
- Pneumococci
Diplococcus pneumoniae
- Streptococci
Streptococcus pyogenes
Streptococcus salivarius
- Staphylococcus
Staphylococcus aureus
Staphylococcus albus
- Pseudomonas
Pseudomonas stutzen
- Neisseria
Neisseria meningitidis
Neisseria gonorrhea
- Enterobacteriaceae
Escherichia coli
Aerobacteria aerogenes
Klebsiella pneumoniae The coliform bacteria
Salmonella typhosa
Salmonella choleraesuis The Salmonellae
Salmonella typhimurium
Shigellae dysenteriae
Shigellae schmitzii
Shigellae arabinotarda
Shigellae flexneri The Shigellae
Shigellae boydii
Shigellae sonnei
- Other enteric bacilli
Proteus vulgaris
Proteus mirabilis Proteus species
Proteus morgani
Pseudomonas aeruginosa
Alcaligenes faecalis
Vibrio cholerae
- Hemophilus-Bordetella group
Hemophilus influenza, H. ducryi
Hemophilus hemophilus
Hemophilus aegypticus
Hemophilus parainfluenzae
Bordetella pertussis
- Pasteurellae
Pasteurella pestis
Pasteurella tulareusis Brucellae
Brucella melitensis
Brucella abortus
Brucella suis
- Aerobic Spore-Forming Bacilli
Bacillus anthracis
Bacillus subtilis
Bacillus megaterium
Bacillus cereus
- Anaerobic Spore-Forming Bacilli
Clostridium botulinum
Clostridium tetani
Clostridium perfringens
Clostridium novyi
Clostridium septicum
Clostridium histolyticum
Clostridium tertium
Clostridium bifermentans
Clostridium sporogenes
- Mycobacteria
Mycobacterium tuberculosis hominis
Mycobacterium bovis
Mycobacterium avium
Mycobacterium leprae
Mycobacterium paratuberculosis
- Actinomycetes (fungus-like bacteria)
Actinomyces isaeli
Actinomyces bovis
Actinomyces naeslundii
Nocardia asteroides
Nocardia brasiliensis
- The Spirochetes
Treponema pallidum
Treponema pertenue
Treponema carateum
Spirillum minus
Streptobacillus moniliformis
Borrelia recurrens
Leptospira icterohemorrhagiae
Leptospira canicola
- Trypanasomes
- Mycoplasmas
Mycoplasma pneumoniae
- Other pathogens
Listeria monocytogenes
Erysipelothrix rhusiopathiae
Streptobacillus moniliformis
Donvania granulomatis
Bartonella bacillformis
- Rickettsiae (bacteria-like parasites)
Rickettsia prowazekii
Rickettsia mooseri
Rickettsia rickettsii
Rickettsia conori
Rickettsia australis
Rickettsia sibiricus
Rickettsia akari
Rickettsia tsutsugamushi
Rickettsia burnetti
Rickettsia quintana
- Chlamydia (unclassifiable parasites bacterial/viral)
Chlamydia agents (naming uncertain)
- Fungi
Cryptococcus neoformans
Blastomyces dermatidis
Histoplasma capsulatum
Coccidioides immitis
Paracocciclioides brasiliensis
Candida albicans
Aspergillus fumigatus
Mucor corymbifera (Absidia corymbifera)
Rhizopus oryzae
Rhizopus arrhizus Phycomycetes
Rhizopus nigricans
Sporotrichum schenkii
Flonsecaea pedrosoi
Fonsecaea compact
Fonsecacae dermatidis
Cladosporium carrioni
Phialophora verrucosa
Aspergillus nidulans
Madurella mycetomi
Madurella grisea
Allescheria boydii
Phialophora jeanselmei
Microsporum gypsum
Trichophyton mentagrophytes
Keratinomyces ajelloi
Microsporum canis
Trichophyton rubrum
Microsporum adouini
- Viruses
Adenoviruses
- Herpes Viruses
Herpes simplex
Varicella (Chicken pox)
Herpes zoster (Shingles)
Virus B
Cytomegalovirus
- Pox Viruses
Variola (smallpox)
Vaccinia
Poxvirus bovis
Paravaccinia
Molluscum contagiosum
- Picornaviruses
Poliovirus
Coxsackievirus
Echoviruses
Rhinoviruses
- Myxoviruses
Influenza (A, B, and C)
Parainfluenza (1-4)
Mumps virus
Newcastle disease virus
Measles virus
Rinderpest virus
Canine distemper virus
Respiratory syncytial virus
Rubella virus
- Arboviruses
Eastern equine encephalitis virus
Western equine encephalitis virus
Sindbis virus
Chikugunya virus
Semliki forest virus
Mayora virus
St. Louis encephalitis virus
California encephalitis virus
Colorado tick fever virus
Yellow fever virus
Dengue virus
- Reoviruses
Reovirus types 1-3
- Retroviruses
Human immunodeficiency viruses (HIV)
Human T-cell lymphotrophic virus I & II (HTLV)
- Hepatitis
Hepatitis A virus
Hepatitis B virus
Hepatitis nonA-nonB virus
Hepatitis, C, D, E
- Tumor viruses
Rauscher leukemia virus
Gross virus
Maloney leukemia virus
Human papilloma viruses
It would be understood by one of skill in the art that it is generally required to treat samples suspected of containing a particular TNA in such a fashion as to produce fragments that can easily hybridize with the PNA. It may be necessary to treat the test sample to effect release of or to extract the TNA for hybridization, such as by exposing blood or other cells to a hypotonic environment, or otherwise disrupting the sample using more vigorous means. When the TNA is thought to be present in double stranded form, it would naturally be desirable to separate the strands to render the TNA hybridizable in single stranded form by methods well known in the art, including but not limited to heating or limited exposure to alkaline conditions which may be neutralized upon addition of the single stranded PNA to allow hybridization to occur. Methods for preparing RNA targets are well known (see Waterhouse 1993, Mitchell 1992).
Fragmentation of nucleic acid samples containing TNAs is usually required to decrease the sample viscosity and to increase the accessibility of the TNAs to the PNAs. Such fragmentation is accomplished by random or specific means known in the art. Thus, for example, specific nucleases known to cut with a particular frequency in the particular genome being analyzed, may be used to produce fragments of a known average molecular size. In addition, other nucleases, phosphodiesterases, exonucleases and endonucleases, physical shear and sonication are all methods amenable for this purpose. These processes are well known in the art. The use of restriction enzymes for the purpose of DNA fragmentation is generally preferred. However, DNA can also be fragmented by a variety of chemical means such as the use of the following types of reagents: EDTA-Fe(II) (according to Stroebel et al. [1988] J. Am. Chem. Soc. 110:7927; Dervan [1986] Science 232:464); Cu(II)-phenanthroline (according to Chen and Sigman [1987] Science 237:1197); class IIS restriction enzyme (according to Kim et al. [1988] Science 240:504); hybrid DNAse (according to Corey et al. [1989] Biochem. 28:8277); bleomycin (according to Umezawa et al. [1986] J. Antibiot. (Tokyo) Ser. A, 19:200); neocarzinostatin (Goldberg et al. [1981] Second Annual Bristol-Myers Symposium in Cancer Research, Academic Press, New York, p. 163); and methidiumpropyl-EDTA-Fe(II) (according to Hertzberg et al. [1982] J. Am. Chem. Soc. 104:313). Removal of proteins, as by treatment with a protease, is also generally desirable and methods for effecting protein removal from nucleic acid samples, without appreciable loss of nucleic acid, are well known in the art.
The TNAs of the present invention should be long enough so that there is a sufficient amount of double-stranded hybrid flanking the TBR so that a TBA can bind unperturbed by the unligated fragment ends. Typically, fragments in the range of about 10 nucleotides to about 100,000 nucleotides, and preferably in the range of about 20 nucleotides to about 1,000 nucleotides are used as the average size for TNA fragments. Examples of specific TNA sequences that could be detected are sequences complementary to the PNA sequences described herein for detection of normal cellular, abnormal cellular (as in activated oncogenes, integrated foreign genes, genetically defective genes), and pathogen-specific nucleic acid sequences, for which specific nucleic acid binding proteins are known, or which can be produced according to methods described in this disclosure. With reference to
4. Extensions to the PNA using BNAs, their preparation, and signal amplification. Under hybridizing conditions, BNAs can be added that hybridize to the PNAs, PNA-BNA hybrids, BNAs and/or BNA-BNA hybrids. The aforementioned additions can be made in a non-vectorial polymeric fashion or in a vectorial fashion, with a known order of BNAs.
With reference to
With reference to
With reference to
Any of a number of methods may be used to prepare the BNAs, including, e.g., synthesis via known chemistry or via recombinant DNA production methods. In the latter method, an essentially unlimited number of BNAs may be produced simply and inexpensively, for example, by production in prokaryotes (E. coli for example) of a plasmid DNA having multiple repeats of the specific BNA sequences flanked by restriction sites having overhanging ends. In this fashion, for example, the bacteriophage lambda left or right operator sites, or any other DNA or other nucleic acid sequence known to specifically and tightly bind a particular BBA, such as a DNA or RNA binding protein, may be produced in an essentially unlimited number of copies, with each copy flanked by an EcoRI, PstI, BamHIII or any of a number of other common restriction nuclease sites. Alternatively, a polymer at repeated sites may be excised by unique restriction sites not present within the polymer. Large quantities of pBR322, pUC plasmid or other plasmid having multiple copies of these sequences are produced by methods well known in the art, the plasmid cut with the restriction enzyme flanking the polymerized site, and the liberated multiple copies of the operators isolated either by chromatography or any other convenient means known in the art. The BNA, prior to use, is then strand separated and is then amenable for polymerization onto a PNA encoding a single stranded complementary copy of the operator as a 1/2 BBR. The BNAs may be polymerized vectorially onto the PNA by using different restriction enzymes to flank each repeat of the polymer in the plasmid used to produce multiple copies of the BNA. Alternatively, the BNA polymer may be hybridized to the PNA via overhangs at one or both ends of the BNA polymer, without the need to strand separate and anneal each BNA segment. Examples of specific BNA sequences are provided above in the section entitled Description of Sequences, as SEQ ID NOS. 35-36. To stabilize the BNA polymer, DNA ligase may be used to covalently link the hybridized BNAs.
5. The Hairpin Nucleic Acids (HNAs) and their preparation. The HNAs of the present invention comprise at least two principal parts joined together: A single-stranded sequence, which is complementary to a 1/2 BBR, and a double-stranded nucleic acid region formed, under hybridizing conditions, by the self-association of self-complementary sequences within the HNA. With reference to FIG. 1(IIc) of the drawings, the 1/2 BBR in the HNA may be constructed so as to be complementary to the 1/2 BBR sequence in the PNA. With reference to
With reference to
HNAs are constructed that will terminate PNA-BNA hybrids which are constructed from the sequential addition of BNAs to the PNA as illustrated in FIG. (2b). The single stranded 1/2 BBR sequences illustrated in
The self-complementary sequences in the HNA and the loop sequence which links the self-complementary hairpin sequences can be of any composition and length, as long as they do not substantially impede or inhibit the presentation of the single-stranded 1/2 BBR that comprises part of the HNA by the HNA or selectively bind the BBA or the TBA. The loop sequences should be selected so that formation of the loop does not impede formation of the hairpin. An example of an HNA useful in this application is provided as SEQ ID NO. 44 (see Description of Sequences above).
6. The Target Binding Assemblies (TBAs) and their preparation. A TBA may be any substance which binds a particular TBR formed by hybridization of particular TNAs and PNAs, provided that the TBA must have at least the following attributes:
-
- (a) The TBA must bind the TBR(s) in a fashion that is highly specific to the TBR(s) of interest. That is, the TBA must discriminate between TBRs present in the TNA-PNA hybrid and similar duplex sequences formed by PNA-CNA hybrids. The TBA must bind the PNA-CNA hybrid with a sufficiently low avidity that upon washing the TBA-TNA-PNA complex, the PNA-CNA hybrid is displaced and the PNA-TNA hybrid is not displaced;
- (b) The TBA must avidly bind the TBR(s) created by the hybridization of the TNA with the PNA. Binding affinities in the range of 10−5 to about 10−12 or higher are generally considered sufficient. As noted below, in some instances, it might be desirable to utilize a particular TBA which has a very low avidity for a particular TBR, but which has a greatly increased affinity when a particular configuration of multiple TBRs is provided so that the square of the affinity of the TBA for each TBR becomes the affinity of relevance to that particular TBA.
Examples of the DNA binding components useful in the formation of TBAs include, but are not limited to NF-kB, papillomavirus E2 protein, transcription factor SP1, inactive restriction enzymes, antibodies, etc. Each of these proteins has been recognized in the art to contain sequences which bind to particular nucleic acid sequences and the affinities of these interactions are known. Naturally, the method of the instant invention is not limited to the use of these known DNA binding proteins or fragments thereof. From the instant disclosure, it would be apparent to one of ordinary skill that the instant method could easily be applied to the use of novel TBAs exhibiting at least the required attributes noted above. Thus, for example, in WO 92/20698, a sequence specific DNA binding molecule comprising an oligonucleotide conjugate formed by the covalent attachment of a DNA binding drug to a triplex forming oligonucleotide was described. The method of that disclosure could be used to produce novel TBAs for use according to the instant disclosure, provided that the TBAs thus formed meet the criteria described above. In addition, the methods of U.S. Pat. Nos. 5,096,815, 5,198,346, and WO88/06601, herein incorporated by reference, may be used to generate novel TBAs for use according to the method of this invention. Specific antibodies or portions thereof could be used (see for example Blais 1994).
Where the TBA is a protein, or a complex of proteins, it will be recognized that any of a number of methods routine in the art may be used to produce the TBA. The TBA may be isolated from its naturally occurring environment in nature, or if this is impractical, produced by the standard techniques of molecular biology. Thus, using NF-kB as an example, using the DNA binding portions of p50 or p65 subunits, this binding assembly could be produced according to recombinant methods known in the art (see for example Ghosh [1990] Cell 62:1019-1029, describing the cloning of the p50 DNA binding subunit of NF-kB and the homology of that protein to rel and dorsal).
Many DNA and other nucleic acid binding proteins are known which can be used as or in TBAs according to this invention. Once the amino acid sequence of any DNA, RNA:DNA, RNA or other nucleic acid binding protein is known, an appropriate DNA sequence encoding the protein can either be prepared by synthetic means, or a cDNA copy of the mRNA encoding the protein from an appropriate tissue source can be used. Furthermore, genomic copies encoding the protein may be obtained and introns spliced out according to methods known in the art. Furthermore, the TBAs may be chemically synthesized.
Once an appropriate coding sequence has been obtained, site-directed mutagenesis may be used to alter the amino acid sequence encoded to produce mutant nucleic acid binding proteins exhibiting more desirable binding characteristics than those of the original nucleic acid binding protein. As an example of this process, the amino acid sequence of the DNA binding portions of NF-kB can be altered so as to produce an NF-kB′ molecule which more tightly binds the NF-kB binding site (see examples below|HIV-Detect and HIV-Lock).
To provide further insight into this aspect of the invention, the following considerations are to be noted. Using NF-kB as an example, a TBA may be prepared using the naturally occurring NF-kB molecule. However, because this molecule is present in vanishingly small quantities in cells, and because the subunits of this DNA binding protein have been cloned, it would be more reasonable to prepare large quantities of the complex via recombinant DNA means as has already been accomplished for this protein (see for example Ghosh [1990] Cell 62:1019-1029).
NF-kB is a pleiotropic inducer of genes involved in immune, inflammatory and growth regulatory responses to primary pathogenic (viral, bacterial or stress) challenges or secondary pathogenic (inflammatory cytokine) challenges. NF-kB is a dimeric DNA binding protein comprising a p50 and a p65 subunit, both of which contact and bind to specific DNA sequences. In an inactivated state, NF-kB resides in the cellular cytoplasm, complexed with a specific inhibitor, I-kB, to form a cytoplasmic heterotrimer. Upon activation, the inhibitor is decomplexed, and the p50-p65 dimer relocates via a specific nuclear localization signal (NLS) to the cell's nucleus where it can bind DNA and effect its role as a transcriptional activator of numerous genes (see Grimm and Baeuerle [1993] Biochem. J. 290:297-308, for a review of the state of the art regarding NF-kB).
The p50-p65 dirtier binds with picomolar affinity to sequences matching the consensus CJGGAMTNYCC (SEQ ID NO. 117), with slightly different affinities depending on the exact sequence. It is worth noting that homodimers of p50 and p65 have also been observed to occur. These homodimers display different biochemical properties as well as slightly different affinities of binding sequences within and similar to the above consensus. Thus, depending on the desired binding characteristics of the TBA, a p50-p65 heterodimer, a p50-p50 homodimer, or a p65-p65 homodimer or fragments of the aforementioned dimers may be used.
One way in which various novel TBAs may be produced for use according to this invention is shown schematically in
The assembly sequences, exemplified above by cro and CI sequences (SEQ ID NOS. 104-108), comprise any stable oligopeptides which naturally and strongly bond to like sequences. Thus, in the case of cro, it is well known that a dimer of cro binds to the bacteriophage lambda operator sites (Anderson et al. [1981] Nature 290:754-758; Harrison and Aggarwal [1990] Ann. Rev. Biochem. 59:933-969). The monomer units of cro tightly and specifically associate with each other. Thus, by linking DNA recognition unit sequences to the cro sequences, close and tight association is achieved.
The optional linker sequences comprise any amino acid sequence which does not interfere with TBA assembly or nucleic acid binding, and which is not labile so as to liberate the nucleic acid recognition unit from the complete TBA. It is desirable but not necessary that the linker sequences be covalently linked to other binding assembly components. The association should be specific so as to aid in the assembly and manufacture of the binding assemblies. Examples of such sequences include, but are not limited to, such well known sequences as are found linking various domains in structural proteins. Thus, for example, in the lambda repressor protein, there is a linking sequence between the DNA binding domain and the dimerization domain which is useful for this purpose. Many other such sequences are known and the precise sequence thereof is not critical to this invention, provided that routine experimentation is conducted to ensure stability and non-interference with target nucleic acid binding. Examples of such sequences are provided herein as Met Ser and SEQ IN NOS. 99-102. Insertion of specific, known proteolysis sites into these linkers is also an integral part of this invention. The presence of such sites in the linker sequences would provide manufacturing advantages, allowing different molecules to be assembled on the chaperone scaffold. In addition to the nucleic acid recognition units, optional linking sequences, and assembly sequences, the novel TBAs of this invention optionally have asymmetry or PILOT TNA sequences and one or more OSA units. The asymmetry sequences are provided to encourage or prevent certain desirable or undesirable associations. For example, in the event that a TBA having homodimeric p50 DNA recognition units is desired, the asymmetry sequences are provided to disrupt the naturally stronger association of NF-kB p50 subunits and p65 subunits, while not disrupting the assembly sequences from bringing together p50 subunits. Examples of such sequences are provided herein as SEQ ID NOS. 85-92 and SEQ ID NOS. 105 and 106.
In a different configuration, NF-kB p50 subunit sequences are brought into close association with transcription factor SP1 DNA recognition unit sequences. This is desirable in the event that an NF-kB/SP1 binding motif is of significance, as in the HIV LTR where a motif of at least six DNA binding protein recognition sites, two NF-kB, three SP1, and a TATA site are known to exist. Since it is also known that the second NF-kB and first SP1 site are significant to regulation of HIV transcription (Perkins et al. [1993] Embo J. 12:3551-3558), this particular configuration of TBA is useful not only in the detection of HIV, but as a therapeutic or prophylactic against HIV infection (see below). In a similar fashion, the long control region (LCR) of human papillomavirus may be used as a key control region for probing according to this method.
In view of the different elements that can be associated, cassette fashion, according to this method of TBA formation, an essentially unlimited variety of TBAs are produced. In
In yet another configuration, the modular structure shown in
The various sequences described above may either be chemically linked using pure oligopeptide starting materials, or they may be linked through provision of recombinant nucleic acids encoding, via the well known genetic code, the various subelements. In the event of recombinant production, linking cro coding sequences to sequences of nucleic acid recognition units to form TBAs is advantageous because not only does cro act as assembly sequences in the chaperone, it also acts to direct the proper folding of the nucleic acid recognition elements. Exemplary sequences for chaperones are provided herein as SEQ ID NOS. 104-108. Furthermore, in the event that higher order structures comprising multiple binding sites is desired, as in a pentameric NF-kB/NF-kB/SP1/SP1/SP1 TBA, proper design of the asymmetry sequences allows such structures to be made.
In the foregoing fashion, TBAs are prepared which bind to their cognate binding sites with high affinity. For example, the NF-kB DNA binding components of the TBAs of
In view of the foregoing description of directed assembly of nucleic acid binding proteins using assembly and asymmetry (or piloting) sequences, those skilled in the art will recognize that a generally applicable method for assembling protein structures is provided by this invention. The generality of this method is demonstrated further by consideration, by way of further example, of the use of an antibody-epitope interaction in the assembly of desired structures. By way of specificity, a DNA binding protein structure may be assembled by linking an NF-kB p50 subunit to an antigen, such as a circularized (through disulfide bonds) melanocyte stimulating hormone (MSH). This pro-MSH molecule may then be bound by an anti-MSH antibody to provide a novel nucleic acid binding assembly, with the antigen and antibody acting as assembly sequences.
The modular structure provided by
7. The Booster Binding Assemblies (BBAs) their preparation. A BBA may be any substance which binds a particular BBR formed by hybridization of particular PNAs and BNAs, including when multiple BNAs (up to and including “n” BNAs, i.e., BNAn, wherein “n” is theoretically 0-∞, but practically is between about 0 and 100) are polymerized onto the PNA for signal amplification, provided that the BBA must have at least the following attributes:
-
- (a) The BBA must bind the BBRs in a fashion that is highly specific to the BBR of interest. That is, the BBA must discriminate between BBRs present in the PNA-BNA hybrid and similar duplex sequences in BNA-CNA hybrids or other CNAs. Thus, where even a single base mismatch or conformational differences with or without base mismatches occur in the production of the PNA-BNAn or PNA-BNAn-HNA hybrid, the BBA must bind the hybrid with a sufficiently low avidity that upon washing the TBA-TNA-PNA-BNAn complex, the BBA is displaced from the CNA sequences but not the BBR sequences.
- (b) The BBA must avidly bind the BBR(s). Binding affinities in the range of 10−5 to about 10−9 or higher are generally considered sufficient.
Examples of BBAs include, but are not limited to cro, and the bacteriophage lambda repressor protein, CI. In addition, see U.S. Pat. No. 4,556,643, herein incorporated by reference, which suggests other DNA sequences and specific binding proteins such as repressors, histones, DNA modifying enzymes, and catabolite gene activator protein. See also EP 0 453 301, herein incorporated by reference, which suggests a multitude of nucleotide sequence specific binding proteins (NSSBPs) such as the tetracycline repressor, the lac repressor, and the tryptophan repressor. Each of these BBAs has been recognized in the art to bind to particular, known nucleic acid sequences and the affinities of these interactions are known. Naturally, the method of the instant invention is not limited to the use of these known BBAs. From the instant disclosure, one of ordinary skill could easily apply the use of novel BBAs exhibiting at least the required attributes noted above to the instant method.
Examples of novel BBAs useful according to this aspect of the invention include novel proteins based on the motif of a known DNA or RNA or DNA:RNA binding protein such as cro or the λ CI repressor protein. Preferably, such modifications are made to improve the handling of these components of the invention. Thus, it may be desirable to add a high concentration of cro to an assay. One of the negative qualities of cro is that at high concentrations, the binding of cro to its DNA target comes into competition with cro-cro interactions. Thus, for example, a chaperoned or mutated cro may be produced which does not have this shortcoming. Examples of such altered chaperones are SEQ ID NOS. 105-106 and 108. Methods known in the art, such as production of novel target binding proteins using variegated populations of nucleic acids and selection of bacteriophage binding to particular, pre-selected targets (i.e., so-called phage-display technology, see discussion above for production of novel TBAs) may be used to produce such novel BBAs as well as the aforementioned novel TBAs.
Where the BBA is a protein, or a complex of proteins, it will be recognized that any of a number of methods routine in the art may be used to produce the BBA. The BBA may be isolated from its naturally occurring environment in nature, or if this is impractical, produced by the standard techniques of molecular biology. Thus, for example, the sequence of the cro protein is known and any molecular clone of bacteriophage lambda may be used to obtain appropriate nucleic acids encoding cro for recombinant production thereof. In addition, the TBAs described herein may be used as BBAs, provided that different TBAs are used to bind TBRs and BBRs.
8. The use of BBAs and BBRs to localize and amplify the localization of the PNA-TNA-TBA complexes (see
Detection of the bound TBR is accomplished through binding of Booster Nucleic Acids, BNAs, to the 1/2 BBRs present on the PNAs under hybridizing conditions. In this manner, even if only a single TBA-TBR complex is bound to the immobilized TBA, a large, amplified signal may be produced by polymerizing multiple BNAs to the immobilized TNA. Each BNA which binds to the TNA forms a BBR which can be bound by BBAs which, like the TBAs immobilized on the solid surface, may be chosen for their very tight and specific binding to particular nucleic acid structures. Thus, according to this embodiment, the immobilized TBA may contain the DNA binding portion of NF-kB, which very specifically and tightly binds to NF-kB binding sites formed upon hybridization of the TNA and PNA to form such a site.
Because it is well known that there are NF-kB binding sites both in the normal human genome and in the long terminal repeats of human immunodeficiency virus (HIV), this invention provides a method of discriminating between the “normal” human sites and the sites present in cells due to HIV infection. Therefore, in a test designed to determine the presence or absence of HIV DNA in a sample of human DNA, the HIV NF-kB binding sites may be viewed as the TNA, and the normal human NF-kB binding sites may be viewed as CNAs. According to the method of this invention, discrimination between these TNAs and CNAs is accomplished by taking advantage of the fact that in the HIV LTR, there are two NF-kB binding sites, followed by three SP1 sites (see, for example, Koken et al. [1992] Virology 191:968-972), while cellular NF-kB binding sites with the same sequences are not found in tandem.
In cases where the TNA contains more than one 1/2 TBR and it is desirable to pursue the therapeutic and prophylactic applications of the TBAs, it may be desirable to use more than one TBA, each with the capacity to bind a TBR in the TNA-PNA complex. In this case, it may be advantageous to select, as components of the TBAs, DNA-binding or RNA-binding domains with lesser affinity for its TBR than the wild-type DNA-binding or RNA-binding domain. Given that the TBAs which are involved in the binding to the multiple TBRs can either assemble together before binding to their TBRs or assemble together after binding to their TBRs, the individual TBAs will not block the corresponding TBRs in the other genomes than the target genome unless the TBRs are spatially capable of binding the assembled TBA complex. One feature of the multimeric assembly of TBAs which is specifically claimed here as part of this invention is that such a multimeric assembly is expected to have a much reduced affinity for a single site within the TNA. However, since the binding is dramatically increased relative to any one TBA, the TBA complex would be expected to not compete for the binding of any single TBR with the corresponding native proteins in situ but bind tightly to sequences in the PNA-TNA hybrid containing the TBRs for each of the nucleic acid-binding components assembled in the TBA. The TBA complex should be assembled and linkers adjusted in the individual TBAs so as to allow the nucleic acid-binding regions contained in the TBA complex to simultaneously reach and bind to these targets.
Once the TNA-PNA hybrids have formed and been contacted with the immobilized TBA, unbound nucleic acid is washed from the immobilized surface and the immobilized hybrids detected. This is accomplished in any one of several ways. In one aspect of this invention, the PNA is labeled with an OSA, such as a radionuclide, colored beads, or an enzyme capable of forming a colored reaction product. Furthermore, in addition to having one or more 1/2 TBRs, the PNA also may contain at least one 1/2 BBR. The 1/2 BBR sequences are chosen so as to be complementary to unique 1/2 BBR sequences in BNAs. In the embodiment described above, for example, where the TBA is NF-kB and the TBR formed upon TNA-PNA hybridization is one or more NF-kB binding sites, the 1/2 BBRs may provide hybridizable (that is, single-stranded, complementary) sequences of the left or right bacteriophage lambda operators (see, for example, Ptashne [1982] Scientific American 247:128-140, and references cited therein for sequences of these operators). These may be polymerized onto the PNA 1/2 BBRs in a vectorial fashion (see
Naturally, those skilled in the art will recognize that the foregoing description is subject to several modifications in the choice of PNAs, TNAs, TBAs, BNAs, and BBAs. Furthermore, in systems other than HIV, those skilled in the art will recognize that the general method described above could be likewise applied. However, these other applications may be simpler than the above described method as the TBAs used may not recognize any normal cellular sites and therefore resort to dimerization or other methods of discriminating between TNAs and CNAs may be less critical. In designing probes and binding assemblies for these other systems, the skilled artisan will be guided by the following principles and considerations.
In the above-described embodiment, the appeal of using the DNA-binding portions of NF-kB protein as the TBA and the NF-kB recognition binding elements as the TBRs is that these elements form an important “control point” for the replication of HIV. That is, it is known that HIV is required to use NF-kB as a critical feature in its replicative life cycle. Similar control points for other pathogens are chosen and used as a basis for detection according to the methods described herein.
From the foregoing description of general features of this invention and the mode of its operation, one skilled in the art will recognize that there are a multiplicity of specific modes for practicing this invention. By way of example, the method of this invention is adaptable to a method and devices using chromatographic test kits described in U.S. Pat. Nos. 4,690,691 and 5,310,650 (the '691 and '650 patents). In those patents, a porous medium was used to immobilize either a TNA or a capture probe, and a solvent was used to transport a mobile phase containing either a labeled PNA, if the TNA was immobilized, or the TNA, if a capture probe was immobilized, into the “capture zone.” Once the TNA was bound in the capture zone, either by directly immobilizing it or through capture, a labeled PNA was chromatographed through the capture zone and any bound label was detected.
Adapting the instant invention to such a system provides the improvement of using a Target Binding Assembly in the capture zone and therefore, the capture of only perfectly matched TBR sequences or other TBRs representing nucleic acid confirmations specifically bound by the TBA within the TNA-PNA duplexes by virtue of the previously described sensitive discrimination by the TBA between TNAs and CNAs.
Once the TNA-PNA hybrids become bound to the immobilized TBA, the signal is amplified by adding BNAs or chromatographing BNAs through the capture zone. Finally, the signal may be further amplified by adding BBAs or chromatographing labeled BBAs through the capture zone. In this fashion, the ease of performing the analysis steps described in the '691 and '650 patents is improved upon herein by providing the additional ability to increase the specificity and, through amplification, the sensitivity of the method described in those patents. The disclosure of the '691 and '650 patents is herein incorporated by reference for the purpose of showing the details of that method and for the teachings provided therein of specific operating conditions to which the compositions and methods of the instant invention are adaptable.
Those skilled in the art will also recognize that the method of the instant invention is amenable to being run in microtiter plates or to automation. The use of machines incorporating the method of this invention therefore naturally falls within the scope of the instant disclosure and the claims appended hereto. Thus, for example, this invention is adaptable for use in such instruments as Abbott Laboratories' (Abbott Park, Ill.) IMx tabletop analyzer. The IMx is currently designed to run both fluorescent polarization immunoassay (FPZA, see Kier [1983] KCLA 3:13-15) and microparticle enzyme immunoassay (MEZA, see Laboratory Medicine, Vol. 20, No. 1, January 1989, pp. 47-49). The MEZA method is easily transformed into a nucleic acid detection method using the instant invention by using a TBA as a capture molecule coated onto a submicron (<0.5 μm on average) sized microparticle suspended in solution. The microparticles coated with TBA are pipetted into a reaction cell. The IMx then pipettes sample (hybridized PNA-TNA) into the reaction cell, forming a complex with the TBA. After an appropriate incubation period, the solution is transferred to an inert glass fiber matrix for which the particles have a strong affinity and to which the microparticles adhere. Either prior to or after filtering the reaction mixture through the glass fiber matrix, BNAs and BBAs are added, or another signal amplification and detection means is used which depends on specific formation of TNA-PNA hybrids. The immobilized complex is washed and the unbound material flows through the glass fiber matrix.
The bound complexes are detected by means of alkaline phosphatase labeled BBAs or otherwise (radioactively, enzymatically, fluorescently) labeled BBAs. In the case of alkaline phosphatase labeled BBAs, the fluorescent substrate 4-methyl umbelliferyl phosphate or like reagent may be added. Alternatively, the enzyme may be bypassed by directly labeling BBAs with this or a like reagent. In any event, fluorescence or other signal is proportional to the amount of PNA-TNA hybrids present.
The fluorescence is detected on the surface of the matrix by means of a front surface fluorometer as described by the manufacturer of the IMx. With minor adjustments that can be made through routine experimentation to optimize an instrument such as the IMx for nucleic acid hybridization and nucleic acid-TBA interactions, the instant invention is completely adaptable to automated analyses of TNA samples.
9. Other diagnostic applications of this invention. While the foregoing description enables the use of the instant invention in a number of different modes, many additional utilities of this invention are readily appreciated, for example, in a mobility retardation system.
In this embodiment of the invention, an improvement of the well known electrophoretic mobility shift assay (EMSA) is conducted as follows (See
A sample of DNA is fragmented, either through random cleavage or through specific restriction endonuclease treatment. The DNA in the sample is then split into two equal aliquots and a specific TNA is added to the first aliquot but not to the second. The first and second aliquot are then electrophoresed in an acrylamide or agarose gel, and the pattern of DNA bands (either visualized through ethidium bromide binding or through being radioactively labeled prior to electrophoresis is then compared for the two aliquots. Fragments of DNA having binding sites to which the TBA is specific are retarded in their migration through the electrophoretic medium. By using an appropriate TBA, any number of DNA or other nucleic acid sequences may be tracked in this fashion.
In a modification of the EMSA described above, fragmented TNA is hybridized with a PNA and fractionated in a first dimension. The fractionated DNA is then reacted with an appropriate TBA and the change in mobility of the DNA fragments is noted. Enhancement of the retardation is possible by adding BBAs as described above. (See, for example, Vijg and references cited therein for known techniques of two (2) dimensional nucleic acid electrophoresis, to which the instant method may be applied).
10. Therapeutic applications. Because of the very tight and selective nucleic acid binding characteristics of the novel TBAs described herein, therapeutic utilities are contemplated in addition to the diagnostic utilities of these compounds. Thus, a TBA comprising tight and specific binding for the HIV-LTR, by virtue of having an NF-kB p50 and an SP1 DNA recognition unit in close association (see
In view of the foregoing description and the examples which follow, those skilled in the art will appreciate that this disclosure describes and enables various embodiments of this invention, including:
1. A probe nucleic acid (PNA) comprising:
-
- (a) a single-stranded sequence, 1/2 TBR, which is capable of forming, under hybridizing conditions, a hybrid, TBR, with a 1/2 TBR present in a target nucleic acid (TNA);
- (b) zero, one or more, and preferably one to ten single stranded sequences, 1/2 BBR, which is capable of forming, under hybridizing conditions, a hybrid BBR, with a 1/2 BBR present in a booster nucleic acid (BNA); and
- (c) an OSA, which is no attached support and/or indicator, or an attached support or other means of localization, including, but not limited to, attachment to beads, polymers, and surfaces, and/or indicators;
wherein said TBR is capable of binding with high affinity to a TBA, said TBA being a substance capable of discriminating between a paired TBR and a TBR having unpaired nucleotides, and further, wherein said BBR is capable of binding with high affinity to a BBA, said BBA being a substance capable of discriminating between a paired BBR and a BBR having unpaired nucleotides. This embodiment includes TBRs which are nucleic acid binding protein recognition sites, such as the HIV LTR, and other nucleic acid binding protein recognition sites in other pathogens, some of which are noted above. The PNA of this embodiment of the invention may produce a TBR which is a nucleic acid binding protein recognition site present in the genome of a pathogen or is a binding site associated with a pathogenic condition in the human genome or a contaminant in a fermentation process.
2. A booster nucleic acid (BNA) comprising:
-
- (a) a 1/2 BBR which has a sequence which is complementary to a 1/2 BBR sequence in a PNA or another BNA already hybridized to the PNA and which is capable of forming, under hybridizing conditions, a hybrid, BBR, with the PNA;
- (b) an OSA attached support or other means of localization, including, but not limited to, attachment to beads, polymers, and surfaces, and/or indicators; and
- (c) additional hybridization sites, 1/2 BBRs, for hybridization with additional BNAs;
wherein said BBR is capable of binding with high affinity to a BBA, said BBA being a substance capable of discriminating between a paired BBR and a BBR having unpaired nucleotides.
3. A Hairpin Nucleic Acid (HNA) comprising a single-stranded sequence, 1/2 BBR, which under hybridizing conditions is capable of forming a hairpin while at the same time binding to a BNA to form a BBR capable of binding a BBA, wherein said BBR is capable of binding with high affinity to a BBA, said BBA being a substance capable of discriminating between a paired BBR and a BBR having unpaired nucleotides.
4. A method for detecting a specific TNA sequence, comprising the steps of:
-
- (a) hybridizing said TNA with a PNA as described above;
- (b) hybridizing said PNA with a BNA containing a 1/2 BBR whose sequence is complementary to a 1/2 BBR sequence in the PNA;
- (c) adding the products of steps (a) and (b) containing a TBR and a BBR, to a surface, liquid or other medium containing a TBA;
- (d) adding BBAs to the mixture in step (c) wherein said BBA comprises:
- (i) a molecule or a portion of a molecule which is capable of selectively binding to a BBR; and
- (ii) a detectable indicator; and
- (e) detecting signal produced by the indicator attached to the BBA. This method includes the use of a protein indicator, including enzymes capable of catalyzing reactions leading to production of colored reaction products. It also includes indicators such as a radionuclide or colored beads.
5. A method for detecting the presence in a sample of a specific Target Nucleic Acid, TNA, which comprises:
-
- (a) contacting said sample with a Probe Nucleic Acid, PNA, which, upon hybridization with said TNA if present in said sample, forms a Target Binding Region, TBR, which is capable of binding a Target Binding Assembly, TBA;
- (b) contacting said sample, already in contact with said PNA, with a TBA capable of binding to any TBRs formed by the hybridization of said PNA and said TNA in the sample.
6. A method for detecting or localizing specific nucleic acid sequences with a high degree of sensitivity and specificity which comprises:
-
- (a) adding PNAs containing a 1/2 BBR and a 1/2 TBR to a sample containing or suspected of containing TNAs containing 1/2 TBR sequences, to form a complex having target binding regions, TBRs, formed by the hybridization of complementary 1/2 TBRs present in the PNAs and TNAs respectively;
- (b) binding the TBRs formed in step (a) to an immobilized TBA to form a TBA-TNA-PNA complex;
- (c) adding Booster Nucleic Acids, BNAs, containing booster binding regions, 1/2 BBRs, to the complex formed in step (b) such that the 1/2 BBRs in the BNAs hybridize with the 1/2 BBR sequences present in the PNAs or to 1/2 BBRs present in BNAs already bound to the PNA, to form BBRs, such that TBA-TNA-PNA-(BNA)n complexes are formed;
- (d) adding Hairpin Nucleic Acids, HNAs, containing 1/2 BBR sequences, to the complex formed in step (c) such that the 1/2 BBRs in the RNAs hybridize with any available 1/2 BBR sequences present in the BNAs of the complex of step (c), thereby capping the extension of the BNAs onto the TBA-TNA-PNA-(BNA)n complexes of step (c) to form TBA-TNA-PNA-(BNA)n-HNA complexes;
- (e) adding Booster Binding Assemblies, BBAs, linked to indicator moieties, to the TBA-TNA-PNA-(BNA)n-HNA complexes formed in step (d) to form TBA-TNA-PNA-(BNA-BBA)n-HNA complexes; and
- (f) detecting the signals produced by the indicator moieties linked to the TBAs, PNAs, BNAs, BBAs or HNAs in the TBA-TNA-PNA-(BNA-BBA)n-HNA complexes of step (e);
- wherein:
- the TNA comprises:
- (i) one or more specific 1/2 TBR nucleic acid sequences, the presence or absence of which in a particular sample is to be confirmed;
- the PNA comprises:
- (i) a single-stranded sequence, 1/2 TBR, which is capable of forming, under hybridizing conditions, a hybrid, TBR, with a 1/2 TBR present in a target nucleic acid (TNA);
- (ii) a single stranded sequence, 1/2 BBR, which is capable of forming, under hybridizing conditions, a hybrid BBR with a 1/2 BBR present in a booster nucleic acid (BNA); and
- (iii) an OSA, which is no attached support and/or indicator, or an attached support or other means of localization, including, but not limited to, attachment to beads, polymers, and surfaces, and/or indicators;
- the BNA comprises:
- (i) a 1/2 BBR, as shown in FIG. 1(IIb), which has a sequence which is complementary to a 1/2 BBR sequence in a PNA and which is capable of forming, under hybridizing conditions, a hybrid, BBR, with the PNA;
- (ii) an OSA attached support or other means of localization, including, but not limited to, attachment to beads, polymers, and surfaces, and/or indicators;
- (iii) additional hybridization sites, 1/2 BBRs, for other BNAs; and
- (iv) sequences, 1/2 BBRs, which can hybridize to BNAs already hybridized to the PNA;
- the BBA comprises:
- (i) a molecule or a portion of a molecule which is capable of selectively binding to a BBR; and
- (ii) no attached support and/or indicator, or an attached support or other means of localization, including, but not limited to, attachment to beads, polymers, and surfaces, and/or indicators;
- and the TBA comprises:
- (i) a molecule or a portion of a molecule which is capable of selectively binding to a TBR; and
- (ii) no attached support and/or indicator, or an attached support or other means of localization, including, but not limited to, attachment to beads, polymers, and surfaces, and/or indicators.
7. An improvement to a solid phase hybridization method for detecting the presence of a target polynucleotide involving: immobilizing a target polynucleotide, if present in a test sample, directly or via an intermediate capture structure, on a solid phase at a capture site; before, during or after said immobilization, attaching a detectable label to said target polynucleotide, if present; and detecting said label, if any, at said capture site; the improvement comprising:
-
- (a) using a Target Binding Assembly, TBA, as the means for achieving immobilization of said target polynucleotide, wherein said TBA binds only to a perfect hybrid formed between a specific Probe Nucleic Acid, PNA, and said target nucleic acid such that a perfect Target Binding Region, TBR, recognizable by said TBA is formed; and
- (b) including in the PNA a single stranded sequence, 1/2 BBR, capable of binding a Booster Nucleic Acid, BNA, containing a single stranded complementary 1/2 BBR which, upon hybridization with the 1/2 BBR in the PNA, forms a BBR capable of binding labeled Booster Binding Assemblies, BBAs.
8. A target binding assembly, TBA, comprising one or more nucleic acid recognition units, linker sequence(s), assembly sequence(s), asymmetry sequence(s), nuclear localization signal sequence(s) (NLS) and OSA(s). The nucleic acid recognition unit may be an NF-kB binding unit, an SP1 binding unit, a TATA binding unit, a human papillomavirus binding unit, an HIV LTR binding unit, or a binding unit for any other fragment of specific sequence the detection of which is desirable and which can be achieved through specific association with the TBA. Such recognition units include, but are not limited to those exemplified herein as SEQ ID NO. 63, SEQ ID NO. 64, SEQ ID NO. 65, SEQ ID NO. 66, SEQ ID NO. 67, SEQ ID NO. 68, SEQ ID NO. 69, SEQ ID NO. 70, SEQ ID NO. 71, SEQ ID NO. 72, and SEQ ID NO. 73. Linker sequences such as oligopeptides which do not interfere with the nucleic acid recognition function of the nucleic acid recognition unit and which provide stability and control over the spacing of the nucleic acid recognition unit from the remainder of the TBA. Examples of such linker sequences are well known in the art and include, but are not limited to oligopeptide sequences from the interdomain primary sequence of a structural protein. Assembly sequences include oligopeptide sequences which direct the folding and association of nucleic acid recognition units. A preferred example of such sequences are oligopeptides derived from the bacteriophage lambda cro protein. The asymmetry sequence directs the association of nucleic acid recognition and assembly sequences in a predetermined order. Such asymmetry sequences are exemplified by sequences derived from insulin, relaxin, gonadotropic hormone, FSH, HCG, LH, ACTH, including but not limited to SEQ ID NOS. 85-92. With reference to
9. Methods of using the novel TBAs of this invention include, but are not limited to a method of using the TBA to bind a particular nucleic acid sequence in a target nucleic acid sample which comprises:
-
- (a) fragmenting the nucleic acid in the target nucleic acid sample;
- (b) contacting, under hybridizing conditions, the fragmented nucleic acid with a probe nucleic acid complementary to the particular nucleic acid sequence of interest, wherein said probe nucleic acid, upon hybridization with said particular nucleic acid sequence of interest forms a target binding region to which said TBA specifically binds.
In this method, the probe nucleic acid, in addition to sequences complementary to said particular nucleic acid sequence of interest, also may have additional sequences to which a booster nucleic acid can bind to form a booster binding site to which a labeled booster binding assembly can bind to provide a signal showing and amplifying the binding of the probe nucleic acid to the target nucleic acid sequence of interest.
An additional aspect of this invention not requiring fragmentation of Target Nucleic Acid, involves administration of the TBA to a patient in need of such treatment of a therapeutically or prophylactically effective amount of said TBA, which comprises administering the TBA, either in the form of a purified protein complex or in the form of a recombinant vector which, upon entry into the patient is able to express the TBA, such that the TBA binds the particular nucleic acid sequence to achieve the desired prophylactic or therapeutic result. This may include providing a dosage which can be determined by routine experimentation to be sufficient to prevent establishment of an active infection by a pathogen. Dosages of purified TBAs may be in the range of about 0.001 to 100 mg/kg. When provided as a recombinant expression vector which will direct the in vivo expression and folding of the TBA, dosages of the recombinant nucleic acid may be substantially lower, particularly if provided in the form of non-pathogenic viral vector. The methods of using the TBAs also include monitoring the shift in mobility of nucleic acids in target nucleic acid samples as a function of the size such that binding of the TBA to a particular fragment in the sample modifies the mobility of the fragment. This aspect of the method provides a useful method of analyzing nucleic acid fragments for particular aberrations, such as might be found associated with metastases.
10. Diagnostic or forensic kits useful in determining the presence of an infection, the susceptibility to a disease, or the origin of a particular nucleic acid containing sample.
11. A method of assembling multimeric TBAs in vivo which comprises introducing nucleic acids encoding component TBAs into a cell. The component TBAs should each contain a nucleic acid recognition unit, assembly sequences, asymmetry sequences, and nuclear localization signal sequences. Linker sequences, optionally included if TBA footprinting experiments indicate the need for such linkers to attain optimal geometry of the multimeric TBA. Upon in vivo expression of each component TBA and proximal binding, via the nucleic acid recognition unit of each component TBA to nucleic acid sequences encountered in the nucleus or elsewhere in the cell, component expressed TBAs are directed to assemble via the included assembly and asymmetry sequences into multimeric TBAs. As described above, such multimeric TBAs will have the advantage of binding specifically with high affinity to TBRs in a specific target sequence, but not at all or with very low affinity to cousin nucleic acids.
The foregoing description of the invention will be appreciated by those skilled in the art to enable preferred embodiments as well as the best mode of this invention. Without limiting the subject matter to the specifics of the examples provided hereinafter, the following examples are provided to further guide those skilled in the art on methods of practicing this invention. Standard recombinant DNA techniques as disclosed in Sambrook, Fritsch, and Maniatis (1989) Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., and more recent texts are not disclosed as these are now well within the skill of the ordinary artisan.
EXAMPLE 1 Preparation of PNAs and Labeling of PNAsProbe nucleic acids, PNAs, may be prepared by means well known in the art. Thus, single stranded polynucleotide PNAs of defined sequence may be prepared via solid phase chemical synthesis according to Merrifield. PNAs may be prepared by automated synthesis using commercially available technology, such as resins and machines produced or marketed by Applied Biosystems, ABI, or other manufacturers. Alternatively, through known recombinant DNA methods, particular PNA sequences are synthesized in vivo, for example by cloning a duplex PNA into a vector which can replicate in E. coli, large quantities of the duplex PNA may be prepared. Multimers of the PNA may be cloned into the vector such that for each mole of vector, several moles of PNA is liberated upon digestion of the vector with a restriction fragment flanking the PNA sequence. Subsequent to synthesis or recombinant production, the PNAs are purified by methods well known in the art such as by gel electrophoresis or high pressure liquid chromatography (HPLC). If the PNA is produced as a duplex, prior to use in a hybridization assay for detection of target nucleic acid sequences, the strands of the PNA are separated by heating or other methods known in the art.
The specific sequence of bases in the PNA is chosen to reflect the sequence to be detected in a TNA, with the proviso that, according to this invention, the PNA contains a 1/2 TBR sequence, which is one that upon hybridization of the PNA and TNA, a TBR is formed. As there are an essentially unlimited number of such sequences known in the art, the choice of the PNA sequence is amenable to selection by the skilled researcher for any given application. The sequence of the HIV LTR is one such sequence, which upon hybridization of a PNA encoding portions of the LTR with TNAs encoding the HIV LTR, TBRs capable of binding the NF-kB or SP1 DNA binding proteins are formed.
In addition to sequences which will form a TBR upon hybridization, the PNA also may contain a 1/2 BBR. This sequence is one which, upon hybridization with a booster nucleic acid, BNA, forms a BBR which is capable of binding a BBA. The BBA is preferably a DNA binding protein having high affinity for the BBR sequence.
In this particular example, hybridization between a PNA having as a 1/2 TBR, SEQ ID NO. 4 and, at the 3′ end of that sequence, a 1/2 BBR sequence shown as SEQ ID NO. 35. The PNA encoding these sequences is either used without labeling or is labeled with a radioactive isotope such as P32, S35, or a similar isotope, according to methods known in the art. Alternatively, the PNA is bound to a bead of between 0.01 to 10 μm, which may be colored for easy visual detection. This label forms the OSA as described in the specification. This probe hybridizes with HIV LTR sequences to form a TBR that binds NF-kB. In addition, the PNA hybridizes with BNAs having a complementary 1/2 BBR to form a bacteriophage lambda left operator that binds either cro or lambda repressor proteins.
In a manner similar to that described above, PNAs are used wherein the 1/2 TBR is any one of SEQ ID NO. 5 or SEQ ID NOS. 7-34, and a 1/2 BBR, such as SEQ ID NO. 35 or SEQ ID NO. 36 is either at the 3′ end or 5′ end of the 1/2 TBR.
EXAMPLE 2 Preparation and Labeling of BNAsSimilar to the methods described in Example 1 for preparation and labeling of PNAs, BNAs are prepared and labeled according to methods known in the art. As described in U.S. Pat. No. 4,556,643, herein incorporated by reference (see particularly Example 1), nucleic acid sequences encoding particular nucleic acid binding sequences may be mass produced by cloning into a replicable vector. Furthermore, similar to that disclosure, the 1/2 TBR and 1/2 BBR sequences may be co-linearly produced in this fashion, with the distinction, however, that according to the instant invention, the 1/2 TBR sequence itself forms a nucleic acid binding component recognition site and the 1/2 BBR, while forming a nucleic acid binding component recognition site, also provides a means of amplifying the signal produced upon binding of the 1/2 TBR to complementary sequences in the TNA by providing for polymerization of BNAs onto the TNA bound PNA. To enable this, a sequence such as SEQ ID NO. 35, which encodes the left operator of bacteriophage lambda, is provided with additional sequences such that an overhang sequence is created on one or both ends of the BNA upon hybridization with the PNA.
As a specific example, vectorial polymerization of BNAs onto a TNA is provided by SEQ ID NOS. 40-43. In this example, SEQ ID NO. 40 encodes two 1/2 TBRs which will hybridize with two 1/2 TBRs in a TNA to form two NF-kB binding sites, while at the same time providing a bacteriophage lambda left operator 1/2 BBR, which additionally is terminated at the 3′ end with the recognition site for the restriction enzyme PstI. Addition of the BNA, SEQ ID NO. 41, with the 1/2 BBR complementary to the 1/2 BBR on the PNA, SEQ ID NO. 40, completes the BBR while at the same time completing the PstI recognition site, leaving a four base overhang for hybridization with additional BNAs. Accordingly, SEQ ID NO. 42 is added which has a four base pair sequence at the 3′ end which is complementary to the four-base overhang remaining from the hybridization of SEQ ID NOS. 40 and 41. In addition, SEQ ID NO. 42 is provided with a five base sequence at its 5′ end which forms part of a BamHI recognition site. The growing polymer of BNAs is extended further by addition of the BNA SEQ ID NO. 43, which is complementary to SEQ ID NO. 42, completing the BBR while at the same time completing the BamHI recognition site and leaving a four base overhang which may be further hybridized with BNAs having complementary sequences. In this fashion, the BNAs may be hybridized extensively so as to greatly amplify the signal of a single PNA-TNA hybridization event.
As with the PNAs described in Example 1, the BNAs may be used in an unlabeled form or may be labeled according to methods known in the art and described in Example 1. It will also be appreciated that, rather than produce the BNA polymer by sequential addition of BNAs to the PNA-TNA complex, the BNA polymer may be preformed and added directly to the PNA-TNA complex. One simple method for preforming such a BNA polymer includes the recombinant production of a vector in which multimers of the BNA are provided with a unique restriction site at either end of the polymer. This polymer of BNAs containing multiple BBRs is cut out of the vector and hybridizes to a single stranded 1/2 BBR remaining in the PNA upon hybridization of the PNA and the TNA. This is accomplished by providing a single stranded sequence in the PNA complementary to an overhang produced in the BNA polymer when it is excised from the production vector.
EXAMPLE 3 Production of HNAs and Their Use for Capping BNA PolymersThe HNAs of this invention are produced according to methods known in the art for polynucleotide production as described in Examples 1 and 2 for PNAs and BNAs. In the production of the HNAs, however, the sequence of the HNA is specifically designed so that a substantial portion of the HNA forms a self-complementary palindrome to form a hairpin, while at the same time, leaving in single stranded form enough bases to be able to hybridize with single stranded sequences in the growing chain of BNAs described in Example 2.
In this Example, a HNA of SEQ ID NO. 44 is provided to cap the extension of BNAs onto the PNA in Example 2 after the addition of the BNA, SEQ ID NO. 43. This is accomplished because SEQ ID NO. 44, while having a palindromic sequence that forms a stable hairpin, also has a sequence at the 5′ end of the HNA which completes the BamHI sequence formed by the hybridization of SEQ ID NO. 42 and SEQ ID NO. 43. Naturally, termination of the polymer after addition of only 3 BNAs is for the purpose of simplicity in demonstrating the invention. As described above, this polymerization may be continued essentially indefinitely to amplify the signal of the PNA-TNA hybridization event. Once the HNA hybridizes to the growing chain of BNAs, the polymer is capped and no further extension of the polymer is possible.
EXAMPLE 4 Preparation of TBAs and BBAs, Labeling, and Immobilization ThereofThe TBAs and BBAs which may be used according to the instant invention include any substance which can specifically bind to the TBRs and BBRs formed by hybridization of the PNAs, TNAs and BNAs. Use of DNA binding proteins forms one example of such substances.
For this example, the TBA is the dimer of the DNA binding portion of p50, and the BBA is the lambda cro protein. These proteins may be produced according to methods known in the art. The genes for both of these proteins have been cloned. Thus, these proteins are recombinantly produced and purified according to methods known in the art. Furthermore, these proteins are labeled, either with a radioisotope, such as radioactive iodine, or with an enzyme, such as beta-galactosidase or horseradish peroxidase, or with a fluorescent dye such as fluorescein or rhodamine, according to methods well known in the art. In addition, either or both of the TBA and BBA may be immobilized on a solid surface such as the surface of a microtiter plate or the surface of a bead, such as a colored bead of diameter anywhere from 0.01 to 10 μm. The labels on the TBAs and BBAs may be the same or different.
In this example, the TBA containing the dimeric p50 DNA binding domain is labeled with rhodamine, while the BBA, cro, is labeled with fluorescein. Accordingly, upon hybridization of the PNAs, TNAs, BNAs and HNAs as described in this patent disclosure and the foregoing and following examples, the nucleic acid hybrids, if formed, are contacted with excess labeled TBA and cro. The fluorescence of these labels is measured according to known methods and, detection of both signals is indicative of the presence of 1/2 TBR sequences in the TNA. The differential signal produced by the fluorescence of the NF-kB and cro is a measure of the degree to which the polymerization of BNAs onto the PNA-TBA hybrid has resulted in amplification of the signal. Amplification from one to over a thousand fold is contemplated according to the method of this invention.
EXAMPLE 5 Hybridization of Two PNAs with a TNA and Discrimination Between a TNA and a CNAThe PNAs, PNA1, SEQ ID NO. 40 and PNA2, SEQ ID NO. 45, are used in about ten-fold molar excess over the concentration of TNAs in a test sample. For this example, an isolated duplex HIV LTR, wherein one strand of which has the sequence SEQ ID NO. 37, shown in
SEQ ID NO. 40 and SEQ ID NO. 45 are both added to separate reactions, the first containing the above described TNA and the second containing the above described CNA. The samples are solubilized in an appropriate hybridization buffer, such as 10 mM Tris (pH 7.5), 1 mM EDTA. The samples arc heated to about 90° C. for about five minutes to strand separate the duplex TNAs and CNAs in the samples, and then the samples are allowed to cool to allow strands of PNAs, TNAs and CNAs to anneal.
Once the hybridization has gone to completion, which can be determined according to known methods such as by calculating the t1/2 based on base compositions and annealing temperature according to known methods, the SEQ ID NO. 40 PNA is polymerized by addition of BNAs as in Example 2 and the SEQ ID NO. 45 PNA2 probe is polymerized with BNAs starting with Sph1 recognition site overhang. Following addition of the BNAs and a brief hybridization period, the separate samples are added to beads coated with covalently immobilized NF-kB, and the NF-kB is allowed to bind to any TBRs formed in the TNA and CNA samples. After about 15 minutes of binding, the samples are washed twice with about three volumes of an appropriate washing buffer, such as 10 mM Tris, pH 7.5, 100 mM NaCl, or another buffer pre-determined not to interfere with NF-kB, or bacteriophage lambda CI repressor protein binding activity. After each wash, the beads are allowed to settle under gravity or by brief centrifugation. This removes any nucleic acids which do not have a perfect NF-kB binding site formed by hybridization of the PNA1 and TNA sequences.
After the final wash, bacteriophage lambda CI repressor protein labeled with a radioactive isotope, such as with radioactive iodine, or labeled with an enzyme, such as horseradish peroxidase, with colored beads, or with a fluorescent label is added to each sample. The samples are then washed several times (about 3) with several volumes (about 2) of an appropriate washing buffer such as 10 mM Tris, pH 7.5, 100 mM NaCl, or another buffer pre-determined not to interfere with NF-kB, or bacteriophage lambda CI repressor protein binding activity. After each wash, the beads are allowed to settle under gravity or by brief centrifugation. Following the last settling or centrifugation, the bound label is quantitated by detecting the bound radioactivity, liberated color in an enzymatic assay, color of bound beads, or fluorescence detection. Alternatively, an anti-CI antibody can be added and a standard sandwich enzyme linked immunoassay or radioimmunoassay performed to detect bound repressor. In addition, as a negative control (background), all of the foregoing manipulations are carried out in tandem with a sample in which beads are used having no immobilized NF-kB.
As a result of the foregoing assay, the control and CNA containing samples have similarly low signals while the TNA containing sample has a signal well above background.
EXAMPLE 6 A Test Kit for the Detection of HIVA. Kit Contents:
-
- 1. Microtiter plate.
- 2. 1 mg/mL solution of recombinantly produced NF-kB in tris-buffered saline.
- 3. Tube containing single stranded HIV PNAs (a mixture of pre-mixed oligonucleotides encoding two NF-kB 1/2 binding sites, i.e. a mixture of SEQ. ID. Nos.7 and 8).
- 4. Tube containing single stranded human genomic PNA, SEQ ID NO. 1.
- 5. Tube of nuclease (PstI).
- 6. Tube of protease.
- 7. Tube containing pre-polymerized BNA's, 100 repeat units of bacteriophage lambda OR, capped with an HNA but with free 1/2 BBRs available for binding to PNA-TNA hybrids.
- 8. Tube of horseradish peroxidase (hrp) conjugated cro.
- 9. Tube of hrp colored substrate.
- 10. Tris buffered saline, 100 mL.
- 11. Lancet.
- 12. Reaction tubes A, B, C, each containing 250 μL of distilled water.
- 13. Medicine dropper.
B. Assay Method:
-
- (a) The microtiter plate (item 1) is coated with the solution of recombinantly produced NF-kB (item 2) at a concentration of 1 mg/mL in tris buffered saline overnight at 4° C. with rocking.
- (b) Three drops of blood of the test taker is obtained by pricking a finger with the lancet (reagent 11), and a drop of blood is dispensed into each of reaction tubes A, B, and C (reagent 12).
- (c) Into each tube is dispensed one drop of protease solution (reagent 6) with the medicine dropper (item 12) and the tube agitated and allowed to sit for 5 minutes.
- (d) One drop of nuclease (item 5) is added to each of tubes A-C using the medicine dropper and the tubes agitated and allowed to sit for 10 minutes.
- (e) One drop of item 3 is added to tube A (test sample); one drop of item 4 is added to tube B (positive control); and one drop of saline (item 12) is added to tube C as a negative control. The tubes are heated to 50° C. In hot water and allowed to cool to room temperature over one hour.
- (f) While the hybridization is allowed to occur in step (d), the excess protein is drained from the surface and the microtiter plate, from step (a), and the plate is rinsed with tris buffered saline (tube 10).
- (g) The contents of tubes A-C from step (e) are transferred to three wells of the microtiter plate and allowed to stand for 1 hour with rocking.
- (h) The microtiter wells containing the contents of tubes A-C are rinsed with tris buffered saline and emptied.
- (i) One drop of item 7 is added to each well and allowed to hybridize with any 1/2 BBR sites bound to the plate, over one hour, followed by three rinses with tris buffered saline.
- (j) One drop of item 8 is added to each well and cro is allowed to bind to any bound RNA's over 10 minutes, followed by five, one mL washes with tris-buffered saline.
- (k) One drop of hrp substrate is added to each well and color allowed to develop.
C. Results:
If wells A and B both show color development, and well C does not, the test is valid and the subject has been infected with HIV. If only well A shows color development, or if well C shows color development, the test has been performed incorrectly, and is invalid. If wells A and C show no color development but well B does, the test is valid and the individual has not been infected with HIV.
EXAMPLE 7 Production of Various Novel TBAsNovel TBAs for use according to the instant invention are prepared as follows:
(a) NFkB/NF-kB (HIV-Detect I). A nucleic acid encoding any one of SEQ ID NOS. 63-71 or a like NF-kB DNA binding protein, is fused, in frame, to a nucleotide sequence encoding an assembly sequence, such as cro, such that the NF-kB DNA recognition sequence is encoded at amino or carboxy terminus of the cro sequence. Optionally, a linker sequence is provided between the NF-kB sequence and the cro sequence. At the other terminus of cro, a nuclear localization signal sequence, such as SEQ ID NO. 72, is optionally provided. Further, asymmetry sequences are optionally provided at the cro terminus unused by the NF-kB recognition sequence. Examples of complete TBAs are shown below.
(b) NF-kB/SP1 (HIV-Detect II). In a similar fashion to that described in (a) above, a recombinant coding sequence encoding an NF-kB recognition domain is prepared. In a separate construct, instead of SEQ ID NOS. 63-72, the coding sequence for the DNA recognition portion of SP1 is included. Such a sequence should encode all or a functional part of SEQ ID NO. 73, which is that portion of the SP1 transcription factor exhibiting DNA binding (see Kadonaga et al. [1987] Cell 51:1079-1090). The NF-kB-encoding vector and the SP1-encoding vector are then co-transfected into an appropriate expression system such as is well known in the art. A monomeric NF-kB recognition unit is added to complete the NF-kB recognition dimer after the assembly of the SP1 and NF-kB recognition units by the chaperone. The asymmetry sequences prevent the formation of NF-kB or SP1 dimers and direct, instead, the formation of NFkB-SP1 heterodimers (i.e., HIV-Detect II), which are then isolated from the expression system (mammalian or bacterial cells) by known methods.
(c) SP1/SP1 TBAs (HIV-Detect III). As described in (b) above, an SP1-encoding TBA construct is prepared. However, only this construct is transfected into the expression system, and asymmetry sequences allowing the formation of SP1-SP1 dimers are included.
(d) SP1-TATA (HIV-Detect IV). As described in (b) above, an SP1-encoding TBA recombinant is produced. In addition, a recombinant encoding a TBA having the binding sequence, SEQ ID NO. 74, or like sequence encoding a TATA recognition unit is prepared with asymmetry sequences complementary to those included in the SP1 TBA-encoding construct. These constructs are co-transfected and the heterodimers isolated by standard methods, including affinity purification on a DNA column having the appropriate SP1-TATA target binding regions.
(e) SP1-E2 (HPV-Detect I). An SP1-encoding construct is prepared as in (b) above. An E2 TBA-encoding construct is prepared by using a sequence encoding any one of SEQ ID NOS. 75-84 and 94-98 which are papillomavirus E2 DNA recognition units (see Hegde et al. [1992] Nature 359:505-512) or like recognition units, is prepared and co-transformed or co-transfected with the SP1 TBA-encoding construct. Monomeric E2 recognition unit is added to the complete E2 recognition dimer after the assembly of the E2-SP1 recognition unit by the chaperone. The heterodimer HPV-Detect I is isolated according to known methods.
(f) E2-E2 (HPV-Detect II). As described above in (e), an E2 TBA-encoding construct is prepared, except that asymmetry sequences are included which permit the formation of E2 dimers. The expressed dimers are then isolated by known methods including affinity for a dimeric E2 binding site on a DNA affinity column.
(g) E2-TATA (HPV-Detect III). As described above in (e) and (d), E2 and TATA binding TBAs are prepared (respectively), except that asymmetry sequences are included which enhance the formation of heterodimers rather than homodimers. These constructs are then co-expressed and the heterodimers are isolated.
(h) TATA-TATA (HPV-Detect IV). As described above in (a) and (d), a TATA binding TBA-encoding construct is prepared using asymmetry sequences that encourage this homodimer formation and the homodimer is isolated.
(i) Other TBAs. As described above for HIV and HPV TBAs, TBAs for any given pathogen or disease state may be produced by identifying specific DNA binding proteins and forming an expression construct using appropriate linker, assembly, and asymmetry sequences.
EXAMPLE 8In a similar fashion to the assay described in Example 5, a more stringent assay is produced by using the duplex NF-kB-SP1 binding protein prepared according to Example 6. Accordingly, the probes shown in
By the appropriate use of asymmetry sequences, TBAs are produced which are dimers, trimers, tetrameres, pentamers, or hexamers of particular DNA recognition units. In this fashion, a hexameric TBA is produced by making a first NF-kB p50 dimeric TBA using asymmetry sequences which enable dimer formation. In addition, the asymmetry sequences enable the tetramerization of the p50 dimer with an SP1-SP1 dimer. Finally, additional asymmetry sequences direct the hexamerization with a dimer exhibiting nuclear localization sequences. This is accomplished by incorporating, for example, asymmetry sequences from insulin, which in nature forms hexamers. This hexamer formation is directed by the sequences, SEQ ID NOS. 85 (A) and 86 (B), 87 (A) and 88 (B), 89 (A) and 90 (B), and 91 (A) and 92 (B) (see
Because of the extremely high affinity for the HIV-LTR that can be generated using a multimeric TBA, the compounds having this structure and which can be used for this purpose are referred to herein as “HIV-Lock.”
An optimal HIV-Lock is defined by footprinting (according to methods well known in the art) TBAs bound to TBRs in the HIV LTR to confirm that the binding affinity of each DNA binding protein contributing to the formation of the multimeric TBA complex is downshifted relative to the affinity for any natural target sequence (i.e. CNAs) from which the DNA binding recognition unit of the TBA is derived. Any concomitant loss in binding affinity for the HIV TBRs is more than compensated for upon formation of the multimer as described below.
There may be competition between the binding of each component TBA for its TBR and assembly, via asymmetry sequences to form the multimer. This is obviated by adjusting the linkers between the chaperone and asymmetry sequences in each TBA component such that these competing events are uncoupled. The resultant reduction in the dimensionality of diffusion (effective concentration increase) for the TBA asymmetry and assembly components results in efficient formation of the multimeric complex.
On the basis of the footprinting, the length and composition of linkers is adjusted to achieve optimal discrimination between target HIV sequences and natural sequences. In this fashion, although each component TBA will have a low affinity for CNA and TBR sequences, the multimeric complex will have an extremely high affinity for the now expanded TBR recognized by the multimeric complex (the square of the affinity of each TBR recognized by each component TBA of the multimeric TBA), while still having a low affinity for CNAs. In the same fashion, other multimeric TBA complexes, aside from HIV-Lock, are prepared.
TBAs which can be formed in this fashion include the following sequences, which are assembled by linking either the protein subunits or nucleic acid sequences encoding these subunits, as follows:
wherein groups I-V consist of sequences selected from:
Group Selected from Sequences
-
- I Any of SEQ ID NOS. 85-92
- II Met Ser, link ed to any of SEQ ID NOS 104-106, each of which is linked to SEQ ID NO. 99.
- III SEQ ID NO. 100 linked to any of SEQ ID NOS. 75-84 or 94-98; SEQ ID NO. 101 linked to either SEQ ID NO. 74 or SEQ ID NO. 93; or SEQ ID NO. 102 linked to SEQ ID NO. 74 or SEQ ID NO. 93; or any of SEQ ID NO. 72, 103, 73, or 63-71.
- IV Any of SEQ ID NOS. 104-106.
- V SEQ ID NO. 99.
Specific examples of such TBAs are SEQ ID NOS. 109-116, assembled as follows:
In this fashion, choosing between appropriate asymmetry sequences, assembly sequences, and DNA recognition units, many different TBAs may be formed. Furthermore, sets of these, such as SEQ ID NOS. 114 and 115, will associate with each other but dimers of SEQ ID NO. 114 or 115 will not form due to charge repulsion in the mutated assembly sequences (SEQ ID NO. 104 is cro; SEQ ID NO. 105 is a novel mutated, negatively charged cro, and SEQ ID NO. 106 is a novel mutated, positively charged cro).
Naturally, given the amino acid sequence of these TBAs, one of ordinary skill could produce recombinant nucleic acid clones encoding these, and such recombinant clones naturally form an integral part of this invention.
EXAMPLE 10 HIV Test Using “HIV-LOCK”In much the same method as used in Example 6, the “HIV-LOCK” produced according to Example 9 is used as the TBA, reagent 2, with similar results.
EXAMPLE 11 HIV Test Using “HIV-LOCK” When Testing Blood for DonationWhen the quantity of blood to be tested is not limiting, as when samples of blood for donation are to be tested for HIV contamination, tests similar to Example 6 are run, but for each of tubes A-C, about 5 mL of blood is pelleted in a tabletop centrifuge. Other reagents are scaled up as necessary to handle the larger quantity of TNA present in the sample.
EXAMPLE 12 “HIV-LOCK” as an Anti-HIV Therapeutic Agent“HIV-LOCK” produced according to Example 9 is formulated as a 1 mg/mL solution in liposomes and injected intravenously into a subject who has been tested and confirmed to be infected with HIV. A dose of about 0.1 mg to 100 mg of “HIV-LOCK”/kilogram body mass is infused over a twenty-four hour period and the concentration of HIV p24 in the patient's serum monitored. The treatment is repeated as often as necessary, such as when elevations in the serum p24 occur.
EXAMPLE 13 Use of an HIV-TBA Construct as a TherapeuticA recombinant retroviral or like vector is used to deliver a construct encoding an HIV-LTR binding TBA to an infected patient. The vector encodes a chaperone, such as cro, and sequences DNA for binding portions of p50. The same vector also encodes a chaperone on which an SP1 TBA folds. Asymmetry sequences are provided such that upon co-expression of the p50-TBA and the SP1-TBA in a single HIV infected cell in vivo, an immediate association occurs between these TBAs, while at the same time preventing any association between the DNA binding portion of p50 and endogenous p50 or p65 monomers. NLS sequences are also provided in the TBAs so that, upon dimer formation, the TBA immediately relocates to the nucleus of the cell and binds specifically to integrated HIV sequences, thus preventing any transcription from that locus.
For this purpose, it is desirable to select sequences encoding DNA binding domains such that the expressed monomers are assembled into a TBA which does not bind to natural human sequences. Thus, it is only upon binding of the TBA components to their target sequences that association between all components of the TBA occurs to form a complex which tightly and specifically binds the HIV LTR.
EXAMPLE 14 Diagnostic Test Kit for Human PapillomavirusThis diagnostic for human papillomavirus takes advantage of the known differential between benign and carcinogenic HPV to provide a test which indicates the susceptibility to malignancy in a patient. The papillomaviruses are a group of small DNA viruses associated with benign squamous epithelial cell tumors in higher vertebrates. At least 27 distinct human types of papillomaviruses (HPVs) have been found; many of these have been associated with specific clinical lesions. Four of these, HPV-6, HPV-11, HPV-16, HPV-18, and HPV-33 have been associated with human genital tract lesions. In general, HPV-6 and HPV-11 DNAs have been found associated with benign lesions of the genital tract. HPV-16, HPV-18, and HPV-33 have also been found associated with premalignant and malignant lesions and are transcribed in most cell lines established from cervical carcinomas. HPV-16, HPV-18, and HPV-33 are likely to be only two members of a large set of HPV DNAs associated with malignant human cervical carcinomas.
Animal models have shown that benign papillomavirus lesions can progress to malignant lesions in the presence of a co-carcinogen. HPV DNA has been found in metastases of cervical carcinomas. In malignant cervical lesions, HPV DNA is usually integrated into the human genome, but there may also be extrachromosomal HPV DNA present. Integration of HPV to form the provirus usually results in the disruption of the viral E2 open reading frame (ORF). Despite disruption of the E2 ORF, and examination of cell lines from several cervical carcinomas has shown transcriptionally active and integrated HPV-16 and HPV-18. When HPV-16 genomes which are present in the human cervical carcinoma cell lines SiHa and CaSki have been examined, there are differences found in the integration of HPV-16. In the SiHa line, the single HPV-16 genome integration occurred at bases 3132 and 3384, disrupting the E1 and E2 ORFs with a deletion of 0.3 kb. An additional 50-basepair deletion of HPV-16 DNA resulted in the E2 and E4 OFRs being fused. The 5′ portion of the HPV-16 DNA, consisting of the disrupted E2 ORF, is ligated to continuous human right flanking sequences. In addition, a single additional guanine is detected at nucleotide 1138 in the middle of the E1 ORF. This basepair addition results in the fusion of the E1a and E1b ORB to a single E1 ORF.
The complete genome of HPV-16 is available on GenBank as accession number K02718; the complete genome of HPV-33 is available on GenBank as accession number M12732; the complete genome of HPV-18 is available on GenBank as accession number X05015.
As a preliminary screen, the fact of an HPV infection is established for a given cervical biopsy sample by a simple “yes/no” type of analysis using, for example, any or all of the PNAs SEQ ID NOS. 46-53 and an E2 TBA as described above (i.e., fragment DNA, binding the PNA, immobilize with the TBA, and detect signal with BNAs and BBAs).
Once a biopsy sample is found to be positive for HPV, additional information is obtained as to the malignancy potential of the HPV by analyzing the integration status of the virus in the human genome.
-
- 1. Fragment the DNA in the cervical biopsy sample and hybridize to a blocking probe having the sequence, SEQ ID NO. 60. This probe will bind to all the fragments in the DNA which have not spliced out the 0.3 kb fragment.
- 2. Expose the DNA in the biopsy sample to a PNA having the sequence, SEQ ID NO. 61. This probe will only bind to fragments which have deleted the 0.3 kb fragment (the blocking probe will prevent the looping out of the large deletion segments if present).
- 3. A PNA having SEQ ID NO. 62 is hybridized with SEQ ID NO. 41 to form a BBR which will bind to cro or λ CI repressor as a BBA, leaving a single-stranded portion capable of hybridizing with the TATA site on SEQ ID NO. 61. This added to form a TBR on the 5′ end of the large deletion.
- 4. The TBR is immobilized by a TBA having a TATA binding protein DNA recognition unit.
- 5. The bound fragments are detected by adding BNAs and BBAs as described above.
Detection of signal in this assay indicates that the large fragment is deleted in HPV present in the TNA. Since this deletion is correlated with malignancy, this assay provides insight into the malignancy potential of the HPV infection. This conclusion can be confirmed by performing an analogous assay based on the deletion of the 52-basepair fragment which is also correlated with HPV-induced malignancy.
The TBP recognition unit used in the TBA for this assay may be chosen, for example, from a sequence such as SEQ ID NO. 70 or SEQ ID NO. 93.
EXAMPLE 15 Recombinant HIV-LOCK™ ProductionPhase One—Preparation of DNA to Produce the HIV-Lock™. In vitro mutagenesis of the coding regions of the naturally occurring, cloned components of the HIV-Lock™ which need to be modified is performed with a MutaGene Phagemid kit. The modified protocol includes the use of a Blue-script plasmid containing each of the binding components of HIV-Lock™. These are transformed into competent cells and uracil-containing phagemids are grown. Single stranded DNA is extracted and used as a template for the mutagenic strand. Oligonucleotides containing the desired mutations, including the incorporation of a novel restriction site, are synthesized and treated with polynucleotide kinase and ATP. The kinase treated oligonucleotides are annealed to the single-stranded template, and a mutagenic strand is synthesized and ligated according to the MutaGene protocol, with the exception that Sequenase 2.0 provides the polymerase. Libraries are screened using both g-32P end-labeled nucleotides containing sequences complementary to the introduced mutations and by isolating the plasmid DNA and identifying the mutants by the presence of the introduced restriction site. The mutations are also confirmed by sequencing with a Sequenase kit. The HIV-Lock™ DNA is cloned into the baculovirus expression system with a polyhedron promotor.
Phase Two—Production of HIV-Lock™ Proteins Using Baculovirus. Sf-9 cells are cultured to a pre-determined density (about 1×106 cells/ml, log phase), infected with the baculovirus containing the HIV-Lock™ instructions and harvested to recover the recombinant proteins comprising the HIV-Lock™ In the scale-up process, cultures are expanded from flasks to spinners and subsequently to bioreactors. Following infection the cells are harvested at 12, 24, 36 and 48 hours for the protein. Indices of viability are monitored throughout the entire process.
Phase Three—Purification of the HIV-Lock™ Proteins The harvested proteins are first separated from particulates by flow-through ultracentrifugation to facilitate downstream purification. The centrifuged product is then sterile filtered. Extracts are then centrifuged at 40,000 rpm at 4° C. for 30 minutes and aliquots are immunoprecipitated with polyclonal rabbit antibody against one of the HIV-Lock™ components. Immunoprecipitated proteins are run on an SDS-10% PAGE gel.
Phase Four—Test of HIV-Lock™ Proteins Against HIV DNA Mobility shift assays are carried out using an oligonucleotide probe comprising elements of the HIV long terminal repeat and fragments containing NFKB binding DNA associated with kappa light chain and microglobulin regulation. The oligonucleotide is annealed to its complimentary strand and end-labeled with g-32P ATP.
Footprinting is accomplished by combining small (10−15 M) of radiolabeled HIV LTR DNA with a slightly larger amount of HIV-Lock™ in a buffer at room temperature for 10 minutes. Dithiothreitol is added prior to the addition of protein. Iron (II), EDTA, hydrogen peroxide and sodium ascorbate are added and the reaction mixture is incubated. A quenching agent is added and the products are analyzed suing denaturing gel electrophoresis. This is done for different concentrations of protein. The resulting gel is imaged using a phosphoimager scanner and the resulting high resolution image file is analyzed to abstract the binding affinity of HIV-Lock™ for the HIV DNA relative to cellular DNA.
Multiple design and testing iterations may be used in order to refind binding of HIV-Lock™ and other TBAs for HIV and other organisms. This process makes it possible to design binding assemblies such that the binding assembly is not competitive with the wild type proteins for single binding sites in the genome samples. The development of TBAs for other organisms and TNAs for sequences within these organisms can be made using the aforementioned method. This method is valid when producing binding assemblies for all nucleic acid TBRs including DNA-DNA, DNA-RNA and RNA-RNA hybrids and combinations of these hybrids.
EXAMPLE 16 Method for Identifying Nucleic Acid Binding Molecules for Production of TBAs and BBAs of the InventionIn the method of this invention, target binding assemblies and booster binding assemblies are assembled by identifying nucleic acid binding molecules, and linking the nucleic acid binding portions of the molecules in such a fashion as to achieve TBAs which discriminate between particular target sequences and even closely related sequences. One method for identifying the nucleic acid binding molecules involves the following steps:
- 1. Obtaining a biological sample containing the target nucleic acid. This could be, for example, an organism or a tissue extract infected with a pathogen.
- 2. Fragmenting the sample so as to expose the nucleic acids and to reduce the size complexity of the nucleic acids contained in the sample.
- 3. Contacting a first aliquot of the fragmented nucleic acids with a control buffer medium and contacting a second aliquot of the fragmented nucleic acids with the control buffer medium containing a known profile of nucleic acid binding molecules.
- 4. Analyzing the two aliquots to identify fragments which have altered behavior in the aliquot contacted with the target binding molecules as opposed to the control aliquot. This is accomplished by single dimension gel electrophoresis, two dimension gel electrophoresis, high performance liquid chromatography, paper chromatography or any other means which reveals a different behavior of the nucleic acid fragments when bound to a nucleic acid binding molecule as opposed to when the nucleic acid fragment is unbound.
- 5. Identifying and isolating fragments which do exhibit altered behavior when contacted with the nucleic acid binding molecule and either sequencing the nucleic acid fragment to determine whether known nucleic acid binding molecule motifs are present, or directly identifying the nucleic acid binding molecule bound to the nucleic acid. The latter can be achieved, for example, by contacting a two dimensional grid of the electrophoresed nucleic acids with differentially labeled antibodies which bind to the various nucleic acid binding molecules.
In this method, preferably nucleic acid motifs are used for either diagnostic or therapeutic purposes wherein the target nucleic acid has more than a single utilizable nucleic acid binding molecule target. In this way, a complex target binding assembly can be generated which takes advantage of the proximity of different nucleic acid binding molecular motifs to enhance the specificity of the TBA assembled from the individual nucleic acid binding components identified. The various nucleic acid binding portions of the nucleic acid binding molecules are then assembled into the complete TBAs as described above, for example, for HIV-LOCK™.
EXAMPLE 17 Method of Identifying Specific RNA Sequences in a SampleAccording to the methods and compositions taught in this invention, any nucleic acid sequence can be specifically identified. Identification of target HIV RNA in a sample is achieved by obtaining a sample of a patient's blood or other biological fluid or extract which may contain the HIV RNA, and testing for the presence of TAR binding sites. Tat is a positive regulator of HIV replication which binds to the TAR region of the HIV RNA. The smallest naturally occurring, fully active form of HIV-Tat is 72 amino acids in length, SEQ Id. 118 herein. Tat contains at least two functional domains, and transactivates gene expression from the HIV long terminal repeat (HIV LTR). Tat binds to an RNA stem loop structure formed from the self-hybridization of sequences in TAR, which is just 5′ to the HIV LTR. HIV TAR RNA forms a dinucleotide bulge and two stem-loop structures (Rhim et al. 1994 Virology: 202, 202-211). The Tat (SEQ. Id. 118) binds to this structure with lower avidity than does Tat variants wherein Ala58 is a threonine or where His65 is an Asp residue. (Derse et al., 1993 Virology: 194,530-536). Utilizing these facts in the instant method is accomplished by:
- 1. Fragmenting a biological sample to expose the nucleic acids and reduce the size complexity of the nucleic acids.
- 2. Contacting a TBA with the sample which identifies a hybrid TAR binding protein sequence and a proximate flanking sequence in the HIV genome. The TBA used for this purpose is assembled on cro as the chaperone using Tat as the HIV RNA specific binding molecule. To provide specificity such that cross-talk between the HIV TAR site and closely related TAR sites which may be present due to such other pathogens as cytomegalovirus, the TBA also has an antibody component which recognizes the DNA-RNA hybrid target binding region formed when a probe nucleic acid binds to the HIV LTR RNA.
- 3. Eliminating any “cross-talk” produced by binding of Tat to the TAR region of the HIV RNA due to such contaminants (cousin RNAs) as the CMV TAR sequence by contacting the reaction with excess Tat variant (either the Ala58 to Thr or the His65 to Asp variants) which bind more avidly. In this way, single binding events due to the TBA binding to a cousin RNAs are competed from the nucleic acid sample by the Tat variant. On the other hand, by appropriately selecting the affinity of the double binding achieved as a result of the antibody and Tat, the TBA is not displaced from true targets. This process is illustrated in
FIG. 16 . In another aspect of this same method, the TBA could be one in which, rather than using a variant of Tat, an antibody is used which recognizes this nucleic acid segment, and the TBA used is a double antibody TBA.
In an alternate version of this method, a probe nucleic acid may be used which hybridizes with the HIV LTR RNA. Accordingly, a duplex segment of the LTR sp1 sites can be created as part of the target binding region. This region of the HIV RNA flanks the TAR region which is 5′ to the LTR but is in close proximity thereto. A TBA containing Tat and two Sp1 binding units is chaperoned to provide Tat binding to TAR and Sp1 binding to the Sp1 binding sites. Amplification and detection is then carried out by adding appropriate BNAs, BBAs and HNAs. In yet another alternative, PNAs having Seq. ID. 38 and Seq. ID. 39 (see
Naturally, those skilled in the art will recognize that other TBA and TNA combinations could be used to optimize the methods exemplified herein.
It should be understood that the examples and embodiments described herein are for illustrative purposes only and that various modifications or changes in light thereof will be suggested to persons skilled in the art and are to be included within the spirit and purview of this application and the scope of the appended claims. It will be understood that sequences provided herein are exemplary only and that other like sequences suggested by these could be used in the methods of this invention. It will also be understood that although any sequence provided herein might be designated as linear, it could be used in a circularly or otherwise permuted form and although designated as not being anti-sense, it could be used in the coding or non-coding form or to bind to coding or non-coding complementary sequences.
Claims
1. An isolated target binding assembly (TBA) comprising a first and a second nucleic acid recognition unit, wherein:
- said first nucleic acid recognition unit is capable of binding with specificity to a first target nucleotide sequence contained within a target polynucleotide;
- said second nucleic acid recognition unit is capable of binding with specificity to a second target nucleotide sequence contained within a target polynucleotide; and
- said nucleic acid recognition units are polypeptides and are the same or different.
2. The TBA of claim 1, wherein each said target nucleotide sequence includes at least 7 contiguous nucleotides.
3. The TBA of claim 1, wherein said first nucleic acid recognition unit is capable of binding said first target nucleotide sequence at the same time that said second nucleic acid recognition unit is bound to said second target nucleotide sequence, when said first and second target nucleotide sequences:
- are both contained within the same target polynucleotide; and
- are separated from one another by intervening nucleotide(s).
4. The TBA of claim 3, wherein each said target nucleotide sequence includes at least 7 contiguous nucleotides.
5. The TBA of claim 2, wherein said first and second nucleic acid recognition units are DNA-binding polypeptides.
6. The TBA of claim 2, wherein said first and second nucleic acid recognition units are RNA-binding polypeptides.
7. The TBA of claim 3, wherein said first and second nucleic acid recognition units are DNA-binding polypeptides.
8. The TBA of claim 3, wherein said first and second nucleic acid recognition units are RNA-binding polypeptides.
9. The TBA of claim 4, wherein said first and second nucleic acid recognition units are DNA-binding polypeptides that bind reversibly and non-covalently to double-stranded DNA.
10. The TBA of claim 9, wherein said first and second nucleic acid recognition units, when considered separately, each have a binding affinity Kd for their respective target nucleotide sequence that is numerically greater than 10−6 M.
11. A composition comprising the TBA of claim 1 bound to a target polynucleotide comprising said first target nucleotide sequence and said second target nucleotide sequence, whereby transcription of a gene contained within the target polynucleotide is hindered.
12. The TBA according to claim 1, wherein said TBA comprises a sequence selected from the group consisting of SEQ ID NO: 109, SEQ ID NO: 110, SEQ ID NO: 111, SEQ ID NO: 112, SEQ ID NO: 113, SEQ ID NO: 114, SEQ ID NO: 115, and SEQ ID NO: 116.
13. The TBA according to claim 1, wherein said TBA comprises a sequence selected from the group consisting of: wherein
- A. I+II+III;
- B. IV+V+III; and
- C. IV+III;
- I indicates any of SEQ ID NOS. 85-92;
- II indicates Met Ser, linked to any of SEQ ID NOS 104-106, each of which is linked to SEQ ID NO. 99;
- III indicates SEQ ID NO. 100 linked to any of SEQ ID NOS. 75-84 or 94-98; SEQ ID NO. 101 linked to either SEQ ID NO. 74 or SEQ ID NO. 93; or SEQ ID NO. 102 linked to SEQ ID NO. 74 or SEQ ID NO. 93; or any of SEQ ID NO. 72, 103, 73, or 63-71;
- IV indicates any of SEQ ID NOS. 104-106; and
- V indicates SEQ ID NO. 99.
14. The TBA according to claim 13, wherein said TBA comprises a sequence selected from the group consisting of SEQ ID NOS:104-106.
15. The TBA according to claim 13, wherein said TBA comprises a sequence selected from Group B or Group C.
16. A method of treatment or prophylaxis comprising administering a TBA according to claim 1, or a polynucleotide encoding said TBA, to a patient in need thereof.
17. The TBA according to claim 1, wherein at least one of said first and a second nucleic acid recognition units is a binding domain selected from NF-kB, papillomavirus E2 protein, transcription factor SP1, an inactive restriction enzyme, or an antibody.
18. The TBA according to claim 1, wherein said TBA is HIV-Detect I, HIV-Detect II, HIV Detect-III, HIV-Detect IV, HPV-Detect I, HPV-Detect II, HPV-Detect III, or HPV-Detect IV.
19. The TBA according to claim 1, wherein said TBA is HIV-Lock.
Type: Application
Filed: Oct 6, 2010
Publication Date: Jul 14, 2011
Inventors: Susan Weininger (Seattle, WA), Arthur M. Weininger (Seattle, WA)
Application Number: 12/899,251
International Classification: A61K 38/16 (20060101); C07K 14/00 (20060101); A61K 31/7088 (20060101); A61P 31/18 (20060101); A61P 31/14 (20060101);
