CROSS-REFERENCE The present application claims priority to U.S. patent application Ser. No. 16/295,393, filed Mar. 7, 2019, which claims priority to U.S. patent application Ser. No. 15/948,798, filed Apr. 9, 2018, now U.S. Pat. No. 10,287,575; which claims priority to U.S. patent application Ser. No. 15/948,793, filed Apr. 9, 2018, now U.S. Pat. No. 10,294,473; which claims priority to U.S. patent application Ser. No. 15/632,222, filed Jun. 23, 2017, now U.S. Pat. No. 10,017,760; which claims priority to U.S. Provisional Application Ser. No. 62/354,516, filed Jun. 24, 2016; U.S. Provisional Application Ser. No. 62/367,386, filed Jul. 27, 2016; and U.S. Provisional Application Ser. No. 62/483,930, filed Apr. 10, 2017, the contents of each being hereby incorporated by reference in their entirety.
STATEMENT AS TO FEDERALLY SPONSORED RESEARCH This disclosure was made with the support of the United States government under Contract number DE-SC0008812 by the Department of Energy.
SEQUENCE LISTING The instant application contains a Sequence Listing which has been submitted in XML file format via EFS-Web and is hereby incorporated by reference in its entirety. Said XML copy, created Mar. 6, 2023, is named 14325_002US5_SL.xml and is 666,805 bytes in size.
BACKGROUND OF THE DISCLOSURE Understanding the relationship between a protein's amino acid structure and its overall function continues to be of great practical, clinical, and scientific significance for biologists and engineers. Directed evolution can be a powerful engineering and discovery tool, but the random and often combinatorial nature of mutations makes their individual impacts difficult to quantify and thus challenges further engineering. More systematic analysis of contributions of individual residues or saturation mutagenesis remains labor- and time-intensive for entire proteins and simply is not possible on reasonable timescales for editing of multiple proteins in parallel, such as metabolic pathways or multi-protein complexes, using standard methods.
SUMMARY OF THE DISCLOSURE Disclosed herein are compositions comprising: i) a first donor nucleic acid comprising: a) a modified first target nucleic acid sequence; b) a first protospacer adjacent motif (PAM) mutation; and c) a first guide nucleic acid sequence comprising a first spacer region complementary to a portion of the first target nucleic acid; and ii) a second donor nucleic acid comprising: a) a barcode corresponding to the modified first target nucleic acid sequence; and b) a second guide nucleic acid sequence comprising a second spacer region complementary to a portion of a second target nucleic acid. Further disclosed are compositions wherein the modified first target nucleic acid sequence comprises at least one inserted, deleted, or substituted nucleic acid compared to a corresponding un-modified first target nucleic acid. Further disclosed are compositions wherein the first guide nucleic acid and second guide nucleic acid are compatible with a nucleic acid-guided nuclease. Further disclosed are compositions wherein the nucleic acid-guided nuclease is a Type II or Type V Cas protein. Further disclosed are compositions wherein the nucleic acid-guided nuclease is a Cas9 homologue or a Cpf1 homologue. Further disclosed are compositions wherein the second donor nucleic acid comprises a second PAM mutation. Further disclosed are compositions wherein the second donor nucleic acid sequence comprises a regulatory sequence or a mutation to turn a screenable or selectable marker on or off. Further disclosed are compositions wherein the second donor nucleic acid sequence targets a unique landing site.
Disclosed herein are methods of genome engineering, the method comprising: a) contacting a population of cells with a polynucleotide, wherein each cell comprises a first target nucleic acid, a second target nucleic acid, and a nucleic acid-guided nuclease, wherein the polynucleotide comprises 1) an editing cassette comprising: i) a modified first target nucleic acid sequence; ii) a first protospacer adjacent motif (PAM) mutation; iii) a first guide nucleic acid sequence comprising a spacer region complementary to a portion of the first target nucleic acid and compatible with the nucleic acid-guided nuclease; and 2) a recorder cassette comprising i) a barcode corresponding to the modified first target nucleic acid sequence; and ii) a second guide nucleic acid sequence comprising a second spacer region complementary to a portion of the second target nucleic acid and compatible with the nucleic acid-guided nuclease; b) allowing the first guide nucleic acid sequence, the second guide nucleic acid sequence, and the nucleic acid-guided nuclease to create a genome edit within the first target nucleic acid and the second target nucleic acid. Further disclosed are methods further comprising c) sequencing a portion of the barcode, thereby identifying the modified first target nucleic acid that was inserted within the first target nucleic acid in step a). Further disclosed are methods wherein the nucleic acid-guided nuclease is a CRISPR nuclease. Further disclosed are methods wherein the PAM mutation is not recognized by the nucleic acid-guided nuclease. Further disclosed are methods wherein the nucleic acid-guided nuclease is a Type II or Type V Cas protein. Further disclosed are methods wherein the nucleic acid-guided nuclease is a Cas9 homologue or a Cpf1 homologue. Further disclosed are methods wherein the recorder cassette further comprises a second PAM mutation that is not recognized by the nucleic acid-guided nuclease.
Disclosed herein are methods of selectable recursive genetic engineering comprising a) contacting cells comprising a nucleic acid-guided nuclease with a polynucleotide comprising a recorder cassette, said recorder cassette comprising i) a nucleic acid sequence that recombines into a unique landing site incorporated during a previous round of engineering, wherein the nucleic acid sequence comprises a unique barcode; and ii) a guide RNA compatible with the nucleic acid-guided nuclease that targets the unique landing site; and b) allowing the nucleic acid-guided nuclease to edit the unique landing site, thereby incorporating the unique barcode into the unique landing site. Further disclosed are methods wherein the nucleic acid sequence further comprises a regulatory sequence that turns transcription of a screenable or selectable marker on or off. Further disclosed are methods wherein the nucleic acid sequence further comprises a PAM mutation that is not compatible with the nucleic acid-guided nuclease. Further disclosed are methods wherein the nucleic acid sequence further comprises a second unique landing site for subsequent engineering rounds. Further disclosed are methods wherein the polynucleotide further comprises an editing cassette comprising a) a modified first target nucleic acid sequence; b) a first protospacer adjacent motif (PAM) mutation; and c) a first guide nucleic acid sequence comprising a first spacer region complementary to a portion of the first target nucleic acid, wherein the unique barcode corresponds to the modified first target nucleic acid such that the modified target nucleic acid can be identified by the unique barcode.
Provided herein are compositions comprising i) a first donor nucleic acid comprising: a) a modified first target nucleic acid sequence; b) a mutant protospacer adjacent motif (PAM) sequence; and c) a first guide nucleic acid sequence comprising a first spacer region complementary to a portion of the first target nucleic acid; and ii) a second donor nucleic acid comprising: a) a recorder sequence; and b) a second guide nucleic acid sequence comprising a second spacer region complementary to a portion of the second target nucleic acid. In some aspects, the first donor nucleic acid and the second donor nucleic acid are covalently linked or comprised on a single nucleic acid molecule. Further provided are compositions wherein the modified first target nucleic acid comprises a 5′ homology are and a 3′ homology arm. Further provided are compositions wherein the 5′ homology arm and the 3′ homology arm are homologous to nucleic acid sequence flanking a protospacer complementary to the first spacer region. Further provided are compositions wherein the modified first target nucleic acid sequence comprises at least one inserted, deleted, or substituted nucleic acid compared to a corresponding un-modified first target nucleic acid. Further provided are compositions wherein the first gRNA is compatible with a nucleic acid-guided nuclease, thereby facilitating nuclease-mediate cleavage of the first target nucleic acid. Further provided are compositions wherein the nucleic acid-guided nuclease is a Cas protein, such as a Type II or Type V Cas protein. Further provided are compositions wherein the nucleic acid-guided nuclease is Cas9 or Cpf1. Further provided are compositions wherein the nucleic acid-guided nuclease is MAD2 or MAD7. Further provided are compositions wherein the nucleic acid-guided nuclease is an engineered or non-natural enzyme. Further provided are compositions wherein the nucleic acid-guided nuclease is a engineered or non-natural enzyme derived from Cas9 or Cpf1. Further provided are compositions wherein the nucleic acid-guided nuclease is an engineered or non-natural enzyme that has less than 80% homology to either Cas9 or Cpf1. Further provided are compositions wherein the mutant PAM sequence is not recognized by the nucleic acid-guided nuclease. Further provided are compositions wherein the recorder sequence comprises a barcode. Further provided are compositions wherein the recorder sequence comprises a fragment of a screenable or selectable marker. Further provided are compositions wherein the recorder sequence comprises a unique sequence by which the modified first target nucleic acid sequence is specifically identified. Further provided are compositions wherein the recorder sequence comprises a unique sequence by which the edited cells may be selected or enriched. A first donor nucleic acid can be a cassette, such as an editing cassette as disclosed herein. A second donor nucleic acid can be a cassette, such as a recording cassette as disclosed herein. A first donor nucleic acid and a second donor nucleic acid can be comprised on a single cassette. A first donor nucleic acid and a second donor nucleic acid can be covalently linked. In any of these examples, the elements of the cassette or donor nucleic acids can be contiguous or non-contiguous.
Provided herein are cells comprising an engineered chromosome or polynucleic acid comprising: a first modified sequence; a first mutant protospacer adjacent motif (PAM); a first recorder sequence, the sequence of which uniquely identifies the first modified sequence, wherein the first modified sequence and the first recorder sequence are separated by at least 1 bp. Further provided are cells wherein the first modified sequence and the first recorder sequence are separated by at least 100 bp. Further provided are cells wherein the first modified sequence and the first recorder sequence are separated by at least 500 bp. Further provided are cells wherein the first modified sequence and the first recorder sequence are separated by at least lkbp. Further provided are cells wherein the first recorder sequence is a barcode. Further provided are cells wherein the first modified sequence is within a coding sequence. Further provided are cells wherein the first modified sequence comprises at least one inserted, deleted, or substituted nucleotide compared to an unmodified sequence. Further provided are cells further comprising: a second modified sequence; a second mutant PAM; and a second recorder sequence, the sequence of which uniquely identifies the second modified sequence, wherein the second modified sequence and the second recorder sequence are separated by at least 1 kb. Further provided are cells wherein the first recorder sequence and the second recorder sequence are separated by less than 100 bp. Further provided are cells wherein the second recorder sequence is a barcode. Further provided are cells wherein the second modified sequence is within a coding sequence. Further provided are cells wherein the second modified sequence comprises at least one inserted, deleted, or substituted nucleotide compared to an unmodified sequence. Further provided are cells wherein the first recorder sequence and the second recorder sequence are immediately adjacent to each other or overlapping, thereby generating a combined recorder sequence. Further provided are cells wherein the combined recorder sequence comprises a selectable or screenable marker. Further provided are cells wherein the combined recorder sequence comprises a selectable or screenable marker by which the cells may be enriched or selected.
Provided herein are methods of genome engineering, the method comprising: a) introducing into a population of cells a plurality of polynucleotides, wherein each cell comprises a first target nucleic acid, a second target nucleic acid, and a targetable nuclease, wherein each polynucleotide comprises: i) a modified first target nucleic acid sequence; ii) a mutant protospacer adjacent motif (PAM) sequence; iii) a first guide nucleic acid sequence comprising a guide sequence complementary to a portion of the first target nucleic acid; and (iv) a recorder sequence; b) inserting the modified first target nucleic acid sequence within the first target nucleic acid; c) inserting the recorder sequence within the second target nucleic acid; d) cleaving the first target nucleic acid by the targetable nuclease in cells that do not comprise the mutant PAM sequence, thereby enriching for cells comprising the inserted modified first target nucleic acid sequence. Further provided are methods wherein the recorder sequence is linked to the modified first target nucleic acid. Further provided are methods wherein each polynucleotide further comprises a second mutant PAM sequence. Further provided are methods wherein each polynucleotide further comprises a second guide nucleic acid sequence comprising a guide sequence complementary to a portion of the second target nucleic acid. Further provided are methods wherein the recorder sequence comprises a unique sequence by which the modified first target nucleic acid is specifically identified upon sequencing the recorder sequence. Further provided are methods further comprising e) sequencing the recorder sequence, thereby identifying the modified first target nucleic acid that was inserted within the first target nucleic acid in step b). Further provided are methods wherein inserting the modified first target nucleic acid sequence comprises cleaving the first target nucleic acid by the nuclease complexed with the transcription product of the first guide nucleic acid sequence. Further provided are methods wherein inserting the modified first target nucleic acid sequence further comprises homology-directed repair. Further provided are methods wherein inserting the modified first target nucleic acid sequence further comprises homologous recombination. Further provided are methods wherein the polynucleotide further comprises a second guide nucleic acid sequence comprising a spacer region complementary to a portion of the second target nucleic acid. Further provided are methods wherein inserting the recorder sequence comprises cleaving the second target nucleic acid by the nuclease complexed with the transcription product of the second guide nucleic acid sequence. Further provided are methods wherein inserting the modified first target nucleic acid sequence further comprises homology-directed repair. Further provided are methods wherein inserting the modified first target nucleic acid sequence further comprises homologous recombination. Further provided are methods wherein the targetable nuclease is a Cas protein. Further provided are methods wherein the Cas protein is a Type II or Type V Cas protein. Further provided are methods wherein the Cas protein is Cas9 or Cpf1. Further provided are methods wherein the targetable nuclease is a nucleic acid-guided nuclease. Further provided are methods wherein the targetable nuclease is MAD2 or MAD7. Further provided are methods wherein the mutant PAM sequence is not recognized by the targetable nuclease. Further provided are methods wherein the targetable nuclease is an engineered targetable nuclease. Further provided are methods wherein the mutant PAM sequence is not recognized by the engineered targetable nuclease. Further provided are methods further comprising introducing a second plurality of polynucleotides into a second population of cells comprising the enriched cells from step d), wherein each cell within the second population of cells comprises a third nucleic acid, a fourth target nucleic acid, and a targetable nuclease. Further provided are methods wherein each of the second polynucleotides comprises: i) a modified third target nucleic acid sequence; ii) a third mutant protospacer adjacent motif (PAM) sequence; iii) a third guide nucleic acid sequence comprising a spacer region complementary to a portion of the third target nucleic acid; and (iv) a second recorder sequence. Further provided are methods wherein each second polynucleotide further comprises a fourth mutant PAM sequence. Further provided are methods wherein each second polynucleotide further comprises a fourth guide nucleic acid sequence comprising a guide sequence complementary to a portion of the fourth target nucleic acid. Further provided are methods further comprising: a) inserting the modified third target nucleic acid sequence within the third target nucleic acid; b) inserting the second recorder sequence within the fourth target nucleic acid; c) cleaving the third target nucleic acid by the nuclease in cells that do not comprise the second mutant PAM sequence, thereby enriching for cells comprising the inserted modified third target nucleic acid sequence. Further provided are methods wherein the fourth target nucleic acid is adjacent to the second target nucleic acid. Further provided are methods wherein the inserted first recorder sequence is adjacent to the second recorder sequence, such that sequencing information can be obtained for the first and second recorder sequence from a single sequencing read. Further provided are methods further comprising obtaining sequence information from the first and second recorder sequences within a single sequence read, thereby identifying the modified first and third target nucleic acid sequences inserted into the first and third target nucleic acids respectively.
Provided herein are methods of identifying engineered cells, the method comprising: a) providing cells, wherein each cell comprises a first target nucleic acid, a second target nucleic acid, and a targetable nuclease, b) introducing into the cells a polynucleotide comprising: 1) a first donor nucleic acid comprising i) a modified target nucleic acid sequence; ii) a mutant protospacer adjacent motif (PAM) sequence; and iii) a first guide nucleic acid sequence comprising a first guide sequence complementary to a portion of the first target nucleic acid; and 2) a second donor nucleic acid comprising i) a recorder sequence corresponding to the modified target nucleic acid sequence; and ii) a second guide nucleic acid sequence comprising a second guide sequence complementary to a portion of the second target nucleic acid, c) cleaving the first target nucleic acid by the nuclease in cells that do not comprise the mutant PAM sequence, thereby enriching for cells comprising the modified target nucleic acid sequence, d) repeating steps a)-c) at least one time using the cells enriched for in step c) as the cells for step a) of the following round, wherein the recorder sequence from each round is incorporated adjacent to the recorder sequence from the previous round, thereby generating a record sequence array comprising a plurality of traceable barcodes, and e) sequencing the record sequence, thereby identifying engineered cells comprising a desired combination of modified target nucleic acids. Further provided are methods wherein the second donor nucleic acid further comprises a second mutant PAM sequence. Further provided are methods wherein sequencing the record sequence array comprises obtaining sequence information for each of the plurality of recorder sequences within a single sequencing read. Further provided are methods wherein steps a)-c) are repeated at least once. Further provided are methods wherein steps a)-c) are repeated at least twice. Further provided are methods wherein the recorder sequence is a barcode. Further provided are methods where the first donor nucleic acid and the second donor nucleic acid are covalently linked. A first donor nucleic acid can be a cassette, such as an editing cassette as disclosed herein. A second donor nucleic acid can be a cassette, such as a recording cassette as disclosed herein. A first donor nucleic acid and a second donor nucleic acid can be comprised on a single cassette. A first donor nucleic acid and a second donor nucleic acid can be covalently linked. In any of these examples, the elements of the cassette or donor nucleic acids can be contiguous or non-contiguous.
Provided herein are methods of identifying engineered cells, the method comprising: a) providing cells, wherein each cell comprises a first target nucleic acid, a second target nucleic acid, and a targetable nuclease, b) introducing into the cells a polynucleotide comprising: 1) a first donor nucleic acid comprising i) a modified target nucleic acid sequence; ii) a mutant protospacer adjacent motif (PAM) sequence; and iii) a first guide nucleic acid sequence comprising a first guide sequence complementary to a portion of the first target nucleic acid; and 2) a second donor nucleic acid comprising i) a marker fragment corresponding to the modified target nucleic acid sequence; and ii) a second guide nucleic acid sequence comprising a second guide sequence complementary to a portion of the second target nucleic acid, c) cleaving the first target nucleic acid by the nuclease in cells that do not comprise the mutant PAM sequence, thereby enriching for cells comprising the modified target nucleic acid sequence, d) repeating steps a)-c) at least one time using the cells enriched for in step c) as the cells for step a) of the following round, wherein the marker fragment from each round is incorporated adjacent to the marker fragment from the previous round, thereby generating a complete marker, and e) identifying cells comprising the complete marker, thereby identifying engineered cells comprising a desired combination of modified target nucleic acids. Further provided are methods wherein the second donor nucleic acid further comprises a second mutant PAM sequence. Further provided are methods wherein the complete marker comprises a selectable marker. Further provided are methods wherein the selectable marker comprises an antibiotic resistance marker or an auxotrophic marker. Further provided are methods wherein the complete marker comprises a screenable reporter. Further provided are methods wherein the screenable reporter comprises a fluorescent reporter. Further provided are methods wherein the screenable reporter comprises a gene. Further provided are methods wherein the screenable reporter comprises a promotor or regulatory element. Further provided are methods wherein the promoter or regulatory element turns on or off transcription of a screenable or selectable element. Further provided are methods wherein the screenable reporter comprises a screenable or selectable element which alters a characteristic of a colony comprising the element compared to a colony that does not comprise the element. A first donor nucleic acid can be a cassette, such as an editing cassette as disclosed herein. A second donor nucleic acid can be a cassette, such as a recording cassette as disclosed herein. A first donor nucleic acid and a second donor nucleic acid can be comprised on a single cassette. A first donor nucleic acid and a second donor nucleic acid can be covalently linked. In any of these examples, the elements of the cassette or donor nucleic acids can be contiguous or non-contiguous.
Provided herein are methods of genome engineering, the method comprising: a) introducing into a population of cells a polynucleotide, wherein each cell comprises a first target nucleic acid, a second target nucleic acid, and a targetable nuclease, wherein the polynucleotide comprises: i) a modified first target nucleic acid sequence; ii) a mutant nuclease recognition sequence; iii) a recorder sequence; b) inserting the modified first target nucleic acid sequence within the first target nucleic acid; c) inserting the recorder sequence within the second target nucleic acid; and d) selecting for a phenotype of interest. Further provided are methods wherein the polynucleotide further comprises a second mutant nuclease recognition site. Further provided are methods wherein selecting for a phenotype of interest comprises cleaving the first target nucleic acid by the nuclease in cells that do not comprise the mutant nuclease recognition sequence, thereby enriching for cells comprising the inserted modified first target nucleic acid sequence. Further provided are methods wherein selecting for a phenotype of interest comprises cleaving the second target nucleic acid by the nuclease in cells that do not comprise the second mutant nuclease recognition sequence, thereby enriching for cells comprising the inserted modified first target nucleic acid sequence. Further provided are methods wherein the recorder sequence is linked to the modified first target nucleic acid. Further provided are methods wherein the recorder sequence comprises a unique sequence by which the modified first target nucleic acid is specifically identified upon sequencing the recorder sequence. Further provided are methods further comprising e) sequencing the recorder sequence, thereby identifying the modified first target nucleic acid that was inserted within the first target nucleic acid in step b). Further provided are methods wherein inserting the modified first target nucleic acid sequence comprises homology-directed repair. Further provided are methods wherein inserting the modified first target nucleic acid sequence comprises homologous recombination. Further provided are methods wherein the nuclease is a Cas protein. Further provided are methods wherein the polynucleotide further comprises a first guide nucleic acid sequence comprising a guide sequence complementary to a portion of the first target nucleic acid. Further provided are methods wherein inserting the modified first target nucleic acid sequence comprises cleaving the first target nucleic acid by the nuclease complexed with the transcription product of the first guide nucleic acid sequence. Further provided are methods wherein the polynucleotide further comprises a second guide nucleic acid sequence comprising a guide sequence complementary to a portion of the second target nucleic acid. Further provided are methods wherein inserting the recorder sequence comprises cleaving the second target nucleic acid by the nuclease complexed with the transcription product of the second guide nucleic acid sequence. Further provided are methods wherein inserting the modified first target nucleic acid sequence or the recorder sequence comprises homology-directed repair. Further provided are methods wherein inserting the modified first target nucleic acid sequence or the recorder sequence comprises homologous recombination. Further provided are methods wherein the mutant nuclease recognition sequence comprises a mutant PAM sequence not recognized by the targetable nuclease. Further provided are methods wherein the Cas protein is a Type II or Type V Cas protein. Further provided are methods wherein the targetable nuclease is MAD2. Further provided are methods wherein the mutant PAM sequence is not recognized by MAD2. Further provided are methods wherein the targetable nuclease is MAD7. Further provided are methods wherein the mutant PAM sequence is not recognized by MAD7. Further provided are methods wherein the Cas protein is Cas9. Further provided are methods wherein the mutant PAM sequence is not recognized by Cas9. Further provided are methods wherein the Cas protein is Cpf1. Further provided are methods wherein the mutant PAM sequence is not recognized by Cpf1. Further provided are methods wherein the nuclease is an Argonaute nuclease. Further provided are methods further comprising introducing guide DNA oligonucleotides comprising a guide sequence complementary to a portion of the first target nucleic acid prior to selecting for a phenotype. Further provided are methods wherein the mutant nuclease recognition sequence comprises a mutant target flanking sequence not recognized by the Argonaute nuclease. Further provided are methods wherein the nuclease is a zinc finger nuclease. Further provided are methods wherein the mutant nuclease recognition sequence is not recognized by the zinc finger nuclease. Further provided are methods wherein the nuclease is a transcription activator-like effector nuclease (TALEN). Further provided are methods wherein the mutant nuclease recognition sequence is not recognized by the TALEN.
INCORPORATION BY REFERENCE All publications and patent applications mentioned in this specification are herein incorporated by reference to the same extent as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference.
BRIEF DESCRIPTION OF THE DRAWINGS The patent or application file contains at least one drawing executed in color. Copies of this patent or patent application publication with color drawing(s) will be provided by the Office upon request and payment of the necessary fee.
FIGS. 1A-1C depict an example genetic engineering workflow including target design, plasmid design, and plasmid library generation. Figure discloses SEQ ID NOS 187-190, respectively, in order of appearance.
FIGS. 2A-2D depicts validation data for an example experiment using a disclosed engineering method.
FIGS. 3A-3C depict an example trackable genetic engineering workflow, including a plasmid comprising an editing cassette and a recording cassette, and downstream sequencing of barcodes in order to identify the incorporated edit or mutation. Figure discloses SEQ ID NOS 191-192, respectively, in order of appearance.
FIGS. 3D-3E depict an example trackable genetic engineering workflow, including iterative rounds of engineering with a different editing cassette and recorder cassette with unique barcode (BC) at each round, followed by selection and tracking to confirm the successful engineering step at each round.
FIGS. 4A-4B depict an example of incorporation of a target mutation and PAM mutation using a plasmid comprising an editing cassette. Figure discloses SEQ ID NOS 193, 193, 194, 193, 194, 193, 193, 195, 193, 196, 193, 197, 194, 193, and 198, respectively, in order of appearance.
FIGS. 5A-5B depict an example of a plasmid comprising an editing cassette, designed to incorporate a target mutation and a PAM mutation into a first target sequence, and a recording cassette, designed to incorporate a barcode sequence into a second target sequence. FIG. 5B depicts example data validating incorporation of the editing cassette and recorder cassette and selection of the engineered bacterial cells. FIG. 5A discloses the left column sequences as SEQ ID NOS 201, 200, 201, 200, 200, 200, 200, 200, 200, 200, 200, 201, 202, 200, 200, 200, 200, 200, 200, 200, 202, and 200, respectively, in order of appearance and the right column sequences as SEQ ID NOS 203, 204, 204, 204, 204, 204, 204, 204, 204, 204, 204, 203, 205, 205, 205, 205, 205, 205, 205, 205, 205, and 205, respectively, in order of appearance.
FIG. 6 depicts an example recursive engineering workflow.
FIGS. 7A-7B depict an example plasmid curing workflow for combinatorial engineering and validation of an example experiment using said workflow.
FIGS. 8A-8B depict an example genetic engineering workflow including target design, plasmid design, and plasmid library generation. Figure discloses SEQ ID NOS 187-190, respectively, in order of appearance.
FIGS. 9A-9D depicts validation data for an example genetic engineering experiment.
FIGS. 10A-10F depict an example data set from a genetic engineering experiment.
FIGS. 11A-11C depict an example design and data set from a genetic engineering experiment.
FIGS. 12A-12F depict an example design for a genetic engineering experiment.
FIGS. 13A-13D depict example designed edits to be made by a genetic engineering. Figure discloses SEQ ID NOS 187-190 and 206-207, respectively, in order of appearance.
FIGS. 14A-14B depict an example design for a genetic engineering experiment.
FIGS. 15A-15D depict an example of Cas9 editing efficiency controls. Figure discloses SEQ ID NOS 208-209, respectively, in order of appearance.
FIGS. 16A-16E depict an examples of toxicity of dsDNA cleavage in E. coli.
FIG. 16F-16H depict an example of a transformation and survival assay, and editing and recording efficiencies, with low and high copy plasmids expressing Cas9.
FIGS. 17A-17D depict an example of genetic engineering strategy for gene deletion. FIGS. 17A and 17C disclose SEQ ID NO: 210.
FIGS. 18A-18B depicts an example of editing efficiency controls by cotransformation of guide nucleic acid and linear dsDNA cassettes.
FIGS. 19A-19D depict an example of library cloning analysis and statistics.
FIGS. 20A-20B depict an example of precision of editing cassette tracking of recombineered populations.
FIG. 21 depicts an example of growth characteristics of folA mutations in M9 minimal media
FIGS. 22A-22C depicts an example of enrichment profiles for folA editing cassettes in minimal media.
FIGS. 23A-23F depict an example of validation of identified acrB mutations for improved solvent and antibiotic tolerance.
FIGS. 24A-24D depict an example mutant variant assessment analysis.
FIG. 25 depicts an example of reconstruction of mutations identified by erythromycin selection.
FIGS. 26A-26B depict an example of validation of Crp S28P mutation for furfural or thermal tolerance.
FIGS. 27A-27C depict an example of edit and barcode correlation studies.
FIG. 28 depicts an example of a selectable recording strategy.
FIG. 29 depicts an example of a selectable recording strategy.
FIGS. 30A-30B depict data from a selectable recording experiment. Figure discloses “TCCACTGGTATGCAT” as SEQ ID NO: 211.
FIGS. 31A-31B depict editing and transformation efficiencies from various nucleic acid-guided nucleases from an example experiment.
FIG. 32 depict editing efficiencies of the MAD2 nuclease with various guide nucleic acids.
FIG. 33 depict editing efficiencies of the MAD7 nuclease with various guide nucleic acids.
DETAILED DESCRIPTION OF THE DISCLOSURE While preferred embodiments of the present invention have been shown and described herein, it will be obvious to those skilled in the art that such embodiments are provided by way of example only. Numerous variations, changes, and substitutions will now occur to those skilled in the art without departing from the invention. It should be understood that various alternatives to the embodiments of the invention described herein may be employed in practicing the invention.
Methods and compositions for enabling sophisticated combinatorial engineering strategies to optimize and explore complex phenotypes are provided herein. Many phenotypes of interest to basic research and biotechnology are the result of combinations of mutations that occur at distal loci. For example, cancer is often linked to mutations that influence multiple hallmark gene functions rather than a single chromosomal edit. Likewise, many metabolic and regulatory processes that are the target of continuing engineering efforts require the activities of many proteins acting in concert to produce the phenotypic output of interest. Methods and compositions disclosed herein can provide ways of rapid engineering and prototyping of such functions since they can provide rapid construction and accurate reporting on the mutational effects at many sites in parallel.
The methods and compositions described herein can be carried out or used in any type of cell in which a nucleic acid-guided nuclease system, such as CRISPR or Argonaute, or other targetable nuclease systems, such as TALEN, ZFN, or meganuclease can function (e.g., target and cleave DNA), including prokaryotic, eukaryotic, or archaeal cells. The cell can be a bacterial cell, such as Escherichia spp. (e.g., E. coli). The cell can be a fungal cell, such as a yeast cell, e.g., Saccharomyces spp. The cell can be a human cell. The cell can be an algal cell, a plant cell, an insect cell, or a mammalian cell, including a human cell. Additionally or alternatively, the methods described herein can be carried out in vitro or in cell-free systems in which a nucleic acid guided nuclease system, such as CRISPR or Argonaute, or other nuclease systems, such as TALEN, ZFN, or meganuclease can function (e.g., target and cleave DNA).
Disclosed herein are compositions and methods for genetic engineering. Disclosed are methods and compositions suitable for trackable or recursive genetic engineering. Disclosed method and compositions can use massively multiplexed oligonucleotide synthesis and cloning to enable high fidelity, trackable, multiplexed genome editing at single nucleotide resolution on a whole genome scale.
Trackable Plasmids Methods and compositions can be used to perform high-fidelity trackable editing, for example, at single-nucleotide resolution and can be used to perform editing at a whole genome scale or on episomal nucleic acid molecules. Massively multiplexed oligonucleotide synthesis and/or cloning can be used in combination with a targetable nuclease system, such as a CRISPR system, MAD2 system, MAD7 system, or other nucleic acid-guided nuclease system, for editing.
As used herein, “cassette” often refers to a single molecule polynucleotide. A cassette can comprise DNA. A cassette can comprise RNA. A cassette can comprise a combination of DNA and RNA. A cassette can comprise non-naturally occurring nucleotides or modified nucleotides. A cassette can be single stranded. A cassette can be double stranded. A cassette can be synthesized as a single molecule. A cassette can be assembled from other cassettes, oligonucleotides, or other nucleic acid molecules. A cassette can comprise one or more elements. Such elements can include, as non-limiting examples, one or more of any of editing sequences, recorder sequences, guide nucleic acids, promoters, regulatory elements, mutant PAM sequences, homology arms, primer sites, linker regions, unique landing sites, a cassette, and any other element disclosed herein. Such elements can be in any order or combination. Any two or more elements can be contiguous or non-contiguous. A cassette can be comprised within a larger polynucleic acid. Such a larger polynucleic acid can be linear or circular, such as a plasmid or viral vector. A cassette can be a synthesized cassette. A cassette can be a trackable cassette.
A cassette can be designed to be used in any method or composition disclosed herein, including multiplex engineering methods and trackable engineering methods. An exemplary cassette can couple two or more elements, such as 1) a guide nucleic acid (e.g. gRNAs or gDNAs) designed for targeting a user specified target sequence in the genome and 2) an editing sequence and/or recorder sequence as disclosed herein (e.g. FIG. 1B and FIG. 5A). A cassette comprising an editing sequence and guide nucleic acid can be referred to as an editing cassette. A cassette comprising an editing sequence can be referred to as an editing cassette. A cassette comprising a recorder sequence and a guide nucleic acid can be referred to as a recorder cassette. A cassette comprising a recorder sequence can be referred to as a recorder cassette. In a preferred embodiment, an editing cassette and a recorder cassette are delivered into the cell at the same time. Further, an editing cassette and a recorder cassette may be covalently linked. Further, these elements may be synthesized together by multiplexed oligonucleotide synthesis.
A cassette can comprise one or more guide nucleic acids and editing cassette as a contiguous polynucleotide. In other examples, one or more guide nucleic acids and editing cassette are contiguous. In other examples, one or more guide nucleic acids and editing cassette are non-contiguous. In other examples, two or more guide nucleic acids and editing cassette are non-contiguous.
A cassette can comprise one or more guide nucleic acids, an editing cassette, and a recorder cassette as a contiguous polynucleotide. In other examples, one or more guide nucleic acids, editing cassette, and recorder cassette are contiguous. In other examples, two or more guide nucleic acids, editing cassette, and recorder cassette are contiguous. In other examples, one or more guide nucleic acids, editing cassette, and recorder cassette are non-contiguous. In other examples, two or more guide nucleic acids, editing cassette, and recorder cassette are non-contiguous.
A cassette can comprise one or more guide nucleic acids, one or more editing cassettes, and one or more recorder cassettes as a contiguous polynucleotide. In other examples, one or more guide nucleic acids, one or more editing cassettes, and one or more recorder cassettes are contiguous. In other examples, two or more guide nucleic acids, two or more editing cassettes, and two or more recorder cassettes are contiguous. In other examples, one or more guide nucleic acids, one or more editing cassettes, and one or more recorder cassettes are non-contiguous. In other examples, two or more guide nucleic acids, two or more editing cassettes, and two or more recorder cassettes are non-contiguous.
A cassette can comprise one or more guide nucleic acids and editing sequence as a contiguous polynucleotide. In other examples, one or more guide nucleic acids and editing sequence are contiguous. In other examples, one or more guide nucleic acids and editing sequence are non-contiguous. In other examples, two or more guide nucleic acids and editing sequence are non-contiguous.
A cassette can comprise one or more guide nucleic acids, an editing sequence, and a recorder sequence as a contiguous polynucleotide. In other examples, one or more guide nucleic acids, editing sequence, and recorder sequence are contiguous. In other examples, two or more guide nucleic acids, editing sequence, and recorder sequence are contiguous. In other examples, one or more guide nucleic acids, editing sequence, and recorder sequence are non-contiguous. In other examples, two or more guide nucleic acids, editing sequence, and recorder sequence are non-contiguous.
A cassette can comprise one or more guide nucleic acids, one or more editing sequences, and one or more recorder sequences as a contiguous polynucleotide. In other examples, one or more guide nucleic acids, one or more editing sequences, and one or more recorder sequences are contiguous. In other examples, two or more guide nucleic acids, two or more editing sequences, and two or more recorder sequences are contiguous. In other examples, one or more guide nucleic acids, one or more editing sequences, and one or more recorder sequences are non-contiguous. In other examples, two or more guide nucleic acids, two or more editing sequences, and two or more recorder sequences are non-contiguous.
An editing cassette can comprise an editing sequence. An editing sequence can comprise a mutation, such as a synonymous or non-synonymous mutation, and homology arms (HAs). An editing sequence can comprise a mutation, such as a synonymous or non-synonymous mutation, and homology arms (HAs) designed to undergo homologous recombination with the target sequence at the site of nucleic acid-guided nuclease-mediated double strand break (e.g. FIG. 1B).
A recorder cassette can comprise a recorder sequence. A recorder sequence can comprise a trackable sequence, such as a barcode or marker, and homology arms (HAs). A recorder sequence can comprise a trackable sequence, such as a barcode or marker, and homology arms (HAs) designed to undergo homologous recombination with the chromosome at the site of nucleic acid-guided nuclease-mediated double strand break (e.g. FIG. 1B).
A cassette can encode machinery (e.g. targetable nuclease, guide nucleic acid, editing cassette, and/or recorder cassette as disclosed herein) necessary to induce strand breakage as well as designed repair that can be selectively enriched and/or tracked in cells. A cell can be any cell such as eukaryotic cell, archaeal cell, prokaryotic cell, or microorganisms such as E. coli (e.g. FIG. 2A-2D).
A cassette can comprise an editing cassette. A cassette can comprise a recorder cassette. A cassette can comprise a guide nucleic acid and an editing cassette. A cassette can comprise a guide nucleic acid and a recorder cassette. A cassette can comprise a guide nucleic acid, an editing cassette, and a recorder cassette. A cassette can comprise two guide nucleic acids, an editing cassette, and a recorder cassette. A cassette can comprise more than two guide nucleic acids, one or more editing cassettes, and one or more recorder cassettes. These elements of a cassette can be linked covalently. These elements of a cassette can be contiguous. These elements of a cassette can be contiguous.
A cassette can comprise an editing sequence. A cassette can comprise a recorder sequence. A cassette can comprise a guide nucleic acid and an editing sequence. A cassette can comprise a guide nucleic acid and a recorder sequence. A cassette can comprise a guide nucleic acid, an editing sequence, and a recorder sequence. A cassette can comprise two guide nucleic acids, an editing sequence, and a recorder sequence. A cassette can comprise more than two guide nucleic acids, one or more editing sequences, and one or more recorder sequences. These elements of a cassette can be linked covalently. These elements of a cassette can be contiguous. These elements of a cassette can be contiguous.
Single genome edits can be tracked using sequencing technologies, e.g. short read sequencing technologies (e.g. FIG. 1C), long read sequencing technologies, or any other sequencing technologies known in the art.
In some embodiments, upon transformation, each editing cassette generates the designed genetic modification within the transformed cell. In some examples, the editng cassette can act in trans as a barcode of the genetic mutation introduced by the editing cassette and can enable the tracking of this mutation frequency in a complex population over time and across many different growth conditions (e.g. FIG. 2A-2D and FIG. 1C).
In some examples, a recording cassette inserts the designed trackable sequence, such as a marker or barcode sequence, within the transformed cell. In some examples, the recorder cassette can act in cis as a barcode of the chromosomal mutation and can enable the tracking of this mutation frequency in a complex population over time and across many different growth conditions.
By providing cis and/or trans tracking of designed genomic mutations, the methods provided herein simplify sample preparation and depth of coverage for mapping diversity genome wide, and provide powerful tools for engineering on a genome scale (e.g. FIG. 1C).
A plurality of cassettes can be pooled into a library of cassettes. A library of cassettes can comprise at least 2 cassettes. A library of cassettes can comprise from 5 to a million cassettes. A library of cassettes can comprise at least a million cassettes. It should be understood, that a library of cassettes can comprise any number of cassettes.
A library of cassettes can comprise cassettes that have any combination of common elements and non-common or unique elements as compared to the other cassettes within the pool. For example, a library of cassettes can comprise common priming sites or common homology arms while also containing non-common or unique barcodes. Common elements can be shared by a plurality, majority, or all of the cassettes within a library of cassettes. Non-common elements can be shared by a plurality, minority, or sub-population of cassettes within the library of cassettes. Unique elements can be shared by a one, a few, or a sub-population of cassettes within the library of cassettes, such that it is able to identify or distinguish the one, few, or sub-population of cassettes from the other cassettes within the library of cassettes. Such combinations of common and non-common are advantageous for multiplexing techniques as disclosed herein.
Cassettes disclosed herein can generate the designed genetic modification or insert the designed marker or barcode sequence with high efficiency within a transformed cell. In many examples, the efficiency is greater than 50%. In some examples the efficiency is 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 99%, or 100% (e.g., FIGS. 32A, 32B, and 33).
In some examples, transformation, editing, and/or recording efficiency can be increased by modulating the expression of one or more components disclosed herein, such as a nucleic acid-guided nuclease. Methods for modulating components are disclosed herein and are known in the art. Such methods can include expressing a component, such as a nucleic acid-guided nuclease or CRISPR enzyme of a subject system on a low or high copy plasmid, depending on the experimental design.
Disclosed herein are methods and compositions for generating cassettes. A cassettes can comprise a cassettes as disclosed herein. For example, a cassette can comprise any combination of an editing cassette and/or recorder cassette disclosed herein. Such a cassette can be comprised on a larger polynucleic acid molecule. Such a larger polynucleic acid molecule can be linear or circular, such as a plasmid or viral vector.
An editing cassette can comprise a mutation relative to a target nucleic acid sequence. The editing cassette can comprise sequence homologous to the target sequence flanking the desired mutation or editing sequence. The editing cassette can comprise a region which recognizes, or hybridizes to, a target sequence of a nucleic acid in a cell or population of cells, is homologous to the target sequence of the nucleic acid of the cell and includes a mutation, or a desired mutation, of at least one nucleotide relative to the target sequence.
An editing cassette can comprise a first editing sequence comprising a first mutation relative to a target sequence. A first mutation can comprise a mutation such as an insertion, deletion, or substitution of at least one nucleotide compared to the non-editing target sequence. The mutation can be incorporated into a coding region or non-coding region.
An editing cassette can comprise a second editing sequence comprising a second mutation relative to a target sequence. The second mutation can be designed to mutate or otherwise silence a PAM sequence such that a corresponding nucleic acid guided nuclease or CRISPR nuclease is no longer able to cleave the target sequence. In such cases, this mutation or silencing of a PAM can serve as a method for selecting transformants in which the first editing sequence has been incorporated.
In some examples, an editing cassette comprises at least two mutations, wherein one mutation is a PAM mutation. In some examples, the PAM mutation can be in a second editing cassette. Such a second editing cassette can be covalently linked and can be continuous or non-contiguous to the other elements in the cassette.
An editing cassette can comprise a guide nucleic acid, such as a gRNA encoding gene, optionally operably linked to a promoter. The guide nucleic acid can be designed to hybridize with the targeted nucleic acid sequence in which the editing sequence will be incorporated.
A recording cassette can comprise a recording sequence. A recorder sequence can comprise a barcoding sequence, or other screenable or selectable marker or fragment thereof. The recording sequence can be comprised within a recorder cassette. Recorder cassettes can comprise regions homologous to an insertion site within a target nucleic acid sequence such that the recording sequence is incorporated by homologous recombination or homology-driven repair systems. The site of incorporation of the recording cassette can be comprised on the same DNA molecule as the target nucleic acid to be edited by an editing cassette. The recorder sequence can comprise a barcode, unique DNA sequence, and/or a complete copy or fragment of a selectable or screenable element or marker.
A recorder cassette can comprise a mutation relative to the target sequence. The mutation can be designed to mutate or otherwise silence a PAM sequence such that a corresponding nucleic acid guided nuclease or CRISPR nuclease is no longer able to cleave the target sequence. In such cases, this mutation or silencing of a PAM site can serve as a method for selecting transformants in which the first recording sequence has been incorporated. A recorder cassette can comprise a PAM mutation. The PAM mutation can be designed to mutate or otherwise silence a PAM site such that a corresponding CRISPR nuclease is no longer able to cleave the target sequence. In such cases, this mutation or silencing of a PAM site can serve as a method for selecting transformants in which the recorder sequence has been incorporated.
A recorder cassette can comprise a guide nucleic acid, such as a gene encoding a gRNA. A promoter can be operably linked to a nucleic acid sequence encoding a guide nucleic acid capable of targeting a nucleic acid-guided nuclease to the desired target sequence. A guide nucleic acid can target a unique site within the target site. In some cases, the guide nucleic acid targets a unique landing site that was incorporated in a prior round of engineering. In some cases, the guide nucleic acid targets a unique landing site that was incorporated by a recorder cassette in a prior round of engineering.
A recorder cassette can comprise a barcode. A barcode can be a unique barcode or relatively unique such that the corresponding mutation can be identified based on the barcode. In some examples, the barcode is a non-naturally occurring sequence that is not found in nature. In most examples, the combination of the desired mutation and the barcode within the editing cassette is non-naturally occurring and not found in nature. A barcode can be any number of nucleotides in length. A barcode can be 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, or more than 30 nucleotides in length. In some cases, the barcode is more than 30 nucleotides in length. A barcode can be generated by degenerate oligonucleotide synthesis. A barcode can be rationally designed or user-specified.
A recorder cassette can comprise a landing site. A landing site can serve as a target site for a recorder cassette for a successive engineering round. A landing site can comprise a PAM. A landing site can be a unique sequence. A landing site can be at least about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 35, 40, 45, 50 nucleotides in length. In some cases, the landing site is greater than 50 nucleotides in length.
A recorder cassette can comprise a selectable or screenable marker, or a regulatory sequence or mutation that turns a selectable or screenable marker on or off. In such cases, the turning on or off of a selectable marker can be used of selection or counter-selection, respectively, of iterative rounds of engineering. An example regulatory sequence includes a ribosome-binding site (RBS), though other such regulatory sequences are envisioned. Mutations that turn a selectable or screenable marker on can include any possible start codon that is recognized by the host transcription machinery. A mutation that turns off a selectable or screenable marker includes a mutation that deletes a start codon or one that inserts a premature stop codon or a reading frame shift mutation.
A recorder cassette can comprise one or more of a guide nucleic acid targeting a target site into which the recorder sequence is to be incorporated, a PAM mutation to silence a PAM used by the guide RNA, a barcode corresponding to an editing cassette, a unique site to serve as a landing site for a recorder cassette of a subsequent rounds of engineering, a regulatory sequence or mutation that turns a screenable or selectable marker on or off, these one or more elements being flanked by homology arms that are designed to promote recombination of these one or more elements into the cleaved target site that is targeted by the guide RNA.
A recorder cassette can comprise a first homology arm, a PAM mutation, a barcode, a unique landing site, a regulatory sequence or mutation for a screenable or selectable marker, a second homology arm, and guide RNA. The first homology arm can be an upstream homology arm. The second homology arm can be a downstream homology arm. The homology arms can be homologous to sequences flanking a cleavage site that is targeted by the guide RNA.
A cassette can comprise two guide nucleic acids designed to target two distinct target nucleic acid sequences. In any case, the guide nucleic acid can comprise a single gRNA or chimeric gRNA consisting of a crRNA and trRNA sequences, or alternatively, the gRNA can comprise separated crRNA and trRNAs, or a guide nucleic acid can comprise a crRNA. In other examples, guide nucleic acid can be introduced simultaneously with a trackable polynucleic acid or plasmid comprising an editing cassette and/or recorder cassette. In these cases, the guide nucleic acid can be encoded on a separate plasmid or be delivered in RNA form via delivery methods well known in the art.
A cassette can comprise a gene encoding a nucleic acid-guided nuclease, such as a CRISPR nuclease, functional with the chosen guide nucleic acid. A nucleic acid-guided nuclease or CRISPR nuclease gene can be provided on a separate plasmid. A nucleic acid-guided nuclease or CRISPR nuclease can be provided on the genome or episomal plasmid of a host organism to which a trackable polynucleic acid or plasmid will be introduced. In any of these examples, the nucleic acid-guided nuclease or CRISPR nuclease gene can be operably linked to a constitutive or inducible promotor. Examples of suitable constitutive and inducible promoters are well known in the art. A nucleic acid-guided nuclease or CRISPR nuclease can be provided as mRNA or polypeptide using delivery systems well known in the art. Such mRNA or polypeptide delivery systems can include, but are not limited to, nanoparticles, viral vectors, or other cell-permeable technologies.
A cassette can comprise a selectable or screenable marker, for example, such as that comprised within a recorder cassette. For example, the recorder cassette can comprise a barcode, such as trackable nucleic acid sequence which can be uniquely correlated with a genetic mutation of the corresponding editing cassette, or otherwise identifiably correlated with such a genetic mutation such that sequencing the barcode will allow identification of the corresponding genetic mutation introduced by the editing cassette. In other examples, recorder cassette can comprise a complete copy of or a fragment of a gene encoding an antibiotic resistance gene, auxotrophic marker, fluorescent protein, or other known selectable or screenable markers.
Trackable Plasmid Libraries A trackable library can comprise a plurality of cassettes as disclosed herein. A trackable library can comprise a plurality of trackable polynucleic acids or plasmids comprising a cassette as disclosed herein. A cassette, polynucleotide, or plasmid comprising a recorder sequence or recorder cassette as disclosed herein can be referred to as a trackable cassette, polynucleotide, or plasmid. A cassette, polynucleotide, or plasmid comprising an editing sequence or editing cassette as disclosed herein can be referred to as a trackable cassette, polynucleotide, or plasmid.
In some cases, within the trackable library are distinct editing cassette and recorder cassette combinations that are sequenced to determine which editing sequence corresponds with a given marker or barcode sequence comprised within the recorder cassette. Therefore, when the editing and recorder sequences are incorporated into a target sequence, you can determine the edit that was incorporated by sequencing the recorder sequence. Sequence the recorder sequence or barcode can significantly cut down on sequencing time and cost.
Library size can depend on the experiment design. For example, if the aim is to edit each amino acid within a protein of interest, then the library size can depend on the number (N) of amino acids in a protein of interest, with a full saturation library (all 20 amino acids at each position or non-naturally occurring amino acids) scaling as 19 (or more)×N and an alanine-mapping library scaling as 1×N. Thus, screening of even very large proteins of more than 1,000 amino acids can be tractable given current multiplex oligo synthesis capabilities (e.g. 120,000 oligos). In addition to or as an alternative to activity screens, more general properties with developed high-throughput screens and selections can be efficiently tested using the libraries disclosed herein. It should be readily understood that libraries can be designed to mutate any number of amino acids within a target protein, including 1, at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, etc. up to the total number of amino acids within a target protein. Additionally, select amino acids can be targeted, such as catalytically active amino acids, or those involved in protein-protein interactions. Each amino acid that is targeted for mutation can be mutated into any number of alternate amino acids, such as any other natural or non-naturally occurring amino acid or amino acid analog. In some examples, all targeted amino acids are mutated to the same amino acid, such as alanine. In other cases, the targeted amino acids are independently mutated to any other amino acid in any combination or permutation.
Trackable libraries can comprise trackable mutations in individual residues or sequences of interest. Trackable libraries can be generated using custom-synthesized oligonucleotide arrays. Trackable plasmids can be generated using any cloning or assembly methods known in the art. For example, CREATE-Recorder plasmids can be generated by chemical synthesis, Gibson assembly, SLIC, CPEC, PCA, ligation-free cloning, other in vitro oligo assembly techniques, traditional ligation-based cloning, or any combination thereof.
Recorder sequences, such as barcodes, can be designed in silico via standard code with a degenerate mutation at the target codon. The degenerate mutation can comprise 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, or more than 30 nucleic acid residues. In some examples, the degenerate mutations can comprise 15 nucleic acid residues (N15).
Homology arms can be added to a recorder sequence and/or editing sequence to allow incorporation of the recorder and/or editing sequence into the desired location via homologous recombination or homology-driven repair. Homology arms can be added by synthesis, in vitro assembly, PCR, or other known methods in the art. For example, homology arms can be assembled via overlapping oligo extension, Gibson assembly, or any other method disclosed herein. A homology arm can be added to both ends of a recorder and/or editing sequence, thereby flanking the sequence with two distinct homology arms, for example, a 5′ homology arm and a 3′ homology arm.
The same 5′ and 3′ homology arms can be added to a plurality of distinct recorder sequences, thereby generating a library of unique recorder sequences that each have the same spacer target or targeted insertion site. The same 5′ and 3′ homology arms can be added to a plurality of distinct editing sequences, thereby generating a library of unique editing sequences that each have the same spacer target or targeted insertion site. In alternative examples, different or a variety of 5′ or 3′ homology arms can be added to a plurality of recorder sequences or editing sequences.
A recorder sequence library comprising flanking homology arms can be cloned into a vector backbone. In some examples, the recorder sequence and homology arms are cloned into a recorder cassette. Recorder cassettes can, in some cases, further comprise a nucleic acid sequence encoding a guide nucleic acid or gRNA engineered to target the desired site of recorder sequence insertion. In many cases, the nucleic acid sequences flanking the CRISPR/Cas-mediated cleavage site are homologous or substantially homologous to the homology arms comprised within the recorder cassette.
An editing sequence library comprising flanking homology arms can be cloned into a vector backbone. In some examples, the editing sequence and homology arms are cloned into an editing cassette. Editing cassettes can, in some cases, further comprise a nucleic acid sequence encoding a guide nucleic acid or gRNA engineered to target the desired site of editing sequence insertion. In many cases, the nucleic acid sequences flanking the CRISPR/Cas-mediated cleavage site are homologous or substantially homologous to the homology arms comprised within the editing cassette.
Gene-wide or genome-wide editing libraries can be subcloned into a vector backbone. In some cases, the vector backbone comprises a recorder cassette as disclosed herein. The editing sequence library can be inserted or assembled into a second site to generate competent trackable plasmids that can embed the recording barcode at a fixed locus while integrating the editing libraries at a wide variety of user defined sites.
A recorder sequence and/or cassette can be assembled or inserted into a vector backbone first, followed by insertion of an editing sequence and/or cassette. In other cases, an editing sequence and/or cassette can be inserted or assembled into a vector backbone first, followed by insertion of a recorder sequence and/or cassette. In other cases, a recorder sequence and/or cassette and an editing sequence and/or cassette are simultaneous inserted or assembled into a vector. In other cases, a recorder sequence and/or cassette and an editing sequence and/or cassette are comprised on the same cassette prior to simultaneous insertion or assembly into a vector. In other cases, a recorder sequence and/or cassette and an editing sequence and/or cassette are linked prior to simultaneous insertion or assembly into a vector. In other cases, a recorder sequence and/or cassette and an editing sequence and/or cassette are covalently linked prior to simultaneous insertion or assembly into a vector. In any of these cases, trackable plasmids or plasmid libraries can be generated.
A cassette or nucleic acid molecule can be synthesized which comprises one or more elements disclosed herein. For example, a nucleic acid molecule can be synthesized that comprises an editing cassette and a guide nucleic acid. A nucleic acid molecule can be synthesized that comprises an editing cassette and a recorder cassette. A nucleic acid molecule can be synthesized that comprises an editing cassette, a guide nucleic acid, and a recorder cassette. A nucleic acid molecule can be synthesized that comprises an editing cassette, a recorder cassette, and two guide nucleic acids. A nucleic acid molecule can be synthesized that comprises a recorder cassette and a guide nucleic acid. A nucleic acid molecule can be synthesized that comprises a recorder cassette. A nucleic acid molecule can be synthesized that comprises an editing cassette. In any of these cases, the guide nucleic acid can optionally be operably linked to a promoter. In any of these cases, the nucleic acid molecule can further include one or more barcodes.
Synthesized cassettes or synthesized nucleic acid molecules can be synthesized using any oligonucleotide synthesis method known in the art. For example, cassettes can be synthesized by array based oligonucleotide synthesis. In such examples, following synthesis of the oligonucleotides, the oligonucleotides can be cleaved from the array. Cleavage of oligonucleotides from an array can create a pool of oligonucleotides.
Software and automation methods can be used for multiplex synthesis and generation. For example, software and automation can be used to create 10, 102, 103, 104, 105, 106, or more cassettes, such as trackable cassettes. An automation method can generate trackable plasmids in rapid fashion. Trackable cassettes can be processed through a workflow with minimal steps to produce precisely defined genome-wide libraries.
Cassette libraries, such as trackable cassette libraries, can be generated which comprise two or more nucleic acid molecules or plasmids comprising any combination disclosed herein of recorder sequence, editing sequence, guide nucleic acid, and optional barcode, including combinations of one or more of any of the previously mentioned elements. For example, such a library can comprise at least 2, 3, 4, 5, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 1000, 1500, 2000, 2500, 3000, 3500, 4000, 4500, 5000, 5500, 6000, 6500, 7000, 7500, 8000, 8500, 9000, 9500, 104, 105, 106, 107, 108, 109, 1010, or more nucleic acid molecules or plasmids of the present disclosure. It should be understood that such a library can include any number of nucleic acid molecules or plasmids, even if the specific number is not explicit listed above.
Cassettes or cassette libraries can be sequenced in order to determine the recorder sequence and editing sequence pair that is comprised on each cassette. In other cases, a known recorder sequence is paired with a known editing sequence during the library generation process. Other methods of determining the association between a recorder sequence and editing sequence comprised on a common nucleic acid molecule or plasmid are envisioned such that the editing sequence can be identified by identification or sequencing of the recorder sequence.
Methods and compositions for tracking edited episomal libraries that are shuttled between E. coli and other organisms/cell lines are provided herein. The libraries can be comprised on plasmids, Bacterial artificial chromosomes (BACs), Yeast artificial chromosomes (YACs), synthetic chromosomes, or viral or phage genomes. These methods and compositions can be used to generate portable barcoded libraries in host organisms, such as E. coli. Library generation in such organisms can offer the advantage of established techniques for performing homologous recombination. Barcoded plasmid libraries can be deep-sequenced at one site to track mutational diversity targeted across the remaining portions of the plasmid allowing dramatic improvements in the depth of library coverage (e.g. FIG. 3A).
Trackable Engineering Methods An example of trackable engineering workflow is depicted in FIG. 3A. Each plasmid can encode a recorder cassette designed to edit a site in the target DNA (e.g. FIG. 3A, black cassette). Sites to be targeted can be functionally neutral sites, or they can be a screenable or selectable marker gene. The homology arm (HA) of the recorder cassette can contain a recorder sequence (e.g., FIG. 3B) that is inserted into the recording site during recombineering. Recombineering can comprise DNA cleavage, such as nucleic acid-guided nuclease-mediated DNA cleavage, and repair via homologous recombination. The recorder sequence can comprise a barcode, unique DNA sequence, or a complete copy or fragment of a screenable or selectable marker. In some examples, the recorder sequence is 15 nucleotides. The recorder sequence can comprise less than 10, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 88, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, or more than 200 nucleotides.
Through a multiplexed cloning approach, the recorder cassette can be covalently coupled to at least one editing cassette in a plasmid (e.g., FIG. 3A, green cassette) to generate trackable plasmid libraries that have a unique recorder and editing cassette combination. This trackable library can be sequenced to generate the recorder/edit mapping and used to track editing libraries across large segments of the target DNA (e.g., FIG. 3C). Recorder and editing sequences can be comprised on the same polynucleotide, in which case they are both incorporated into the target nucleic acid sequence, such as a genome or plasmid, by the same recombination event. In other examples, the recorder and editing sequences can be comprised on separate cassettes within the same trackable plasmid, in which case the recorder and editing sequences are incorporated into the target nucleic acid sequence by separate recombination events, either simultaneously or sequentially.
Methods are provided herein for combining multiplex oligonucleotide synthesis with recombineering, to create libraries of specifically designed and trackable mutations. Screens and/or selections followed by high-throughput sequencing and/or barcode microarray methods can allow for rapid mapping of mutations leading to a phenotype of interest.
Methods and compositions disclosed herein can be used to simultaneously engineer and track engineering events in a target nucleic acid sequence.
Trackable plasmids can be generated using in vitro assembly or cloning techniques. For example, the CREATE-Recorder plasmids can be generated using chemical synthesis, Gibson assembly, SLIC, CPEC, PCA, ligation-free cloning, other in vitro oligo assembly techniques, traditional ligation-based cloning, or any combination thereof.
Trackable plasmids can comprise at least one recording sequence, such as a barcode, and at least one editing sequence. In most cases, the recording sequence is used to record and track engineering events. Each editing sequence can be used to incorporate a desired edit into a target nucleic acid sequence. The desired edit can include insertion, deletion, substitution, or alteration of the target nucleic acid sequence. In some examples, the one or more recording sequence and editing sequences are comprised on a single cassette comprised within the trackable plasmid such that they are incorporated into the target nucleic acid sequence by the same engineering event. In other examples, the recording and editing sequences are comprised on separate cassettes within the trackable plasmid such that they are each incorporated into the target nucleic acid by distinct engineering events. In some examples, the trackable plasmid comprises two or more editing sequences. For example, one editing sequence can be used to alter or silence a PAM sequence while a second editing sequence can be used to incorporate a mutation into a distinct sequence.
Recorder sequences can be inserted into a site separated from the editing sequence insertion site. The inserted recorder sequence can be separated from the editing sequence by 1 bp or any number of base pairs. For example, the separation distance can be about 1 bp, 10 bp, 50 bp, 100 bp, 500 bp, lkp, 2 kb, 5 kb, 10 kb, or greater. The separation distance can be any discrete integer of base pairs. It should be readily understood that there the limit of the number of base pairs separating the two insertion sites can be limited by the size of the genome, chromosome, or polynucleotide into which the insertions are being made. In some examples, the maximum distance of separation depends on the size of the target nucleic acid or genome.
Recorder sequences can be inserted adjacent to editing sequences, or within proximity to the editing sequence. For example, the recorder sequence can be inserted outside of the open reading frame within which the editing sequence is inserted. Recorder sequence can be inserted into an untranslated region adjacent to an open reading frame within which an editing sequence has been inserted. The recorder sequence can be inserted into a functionally neutral or non-functional site. The recorder sequence can be inserted into a screenable or selectable marker gene.
In some examples, the target nucleic acid sequence is comprised within a genome, artificial chromosome, synthetic chromosome, or episomal plasmid. In various examples, the target nucleic acid sequence can be in vitro or in vivo. When the target nucleic acid sequence is in vivo, the CREATE-Recorder plasmid can be introduced into the host organisms by transformation, transfection, conjugation, biolistics, nanoparticles, cell-permeable technologies, or other known methods for DNA delivery, or any combination thereof. In such examples, the host organism can be a eukaryote, prokaryote, bacterium, archaea, yeast, or other fungi.
The engineering event can comprise recombineering, non-homologous end joining, homologous recombination, or homology-driven repair. In some examples, the engineering event is performed in vitro or in vivo.
The methods described herein can be carried out in any type of cell in which a nucleic acid-guided nuclease system can function (e.g., target and cleave DNA), including prokaryotic and eukaryotic cells or in vitro. In some embodiments the cell is a bacterial cell, such as Escherichia spp. (e.g., E. coli). In other embodiments, the cell is a fungal cell, such as a yeast cell, e.g., Saccharomyces spp. In other embodiments, the cell is an algal cell, a plant cell, an insect cell, or a mammalian cell, including a human cell.
In some examples, a cell is a recombinant organism. For example, the cell can comprise a non-native nucleic acid-guided nuclease system. Additionally or alternatively, the cell can comprise recombination system machinery. Such recombination systems can include lambda red recombination system, Cre/Lox, attB/attP, or other integrase systems. Where appropriate, the trackable plasmid can have the complementary components or machinery required for the selected recombination system to work correctly and efficiently.
A method for genome editing can comprise: (a) introducing a vector that encodes at least one editing cassette and at least one guide nucleic acid into a first population of cells, thereby producing a second population of cells comprising the vector; (b) maintaining the second population of cells under conditions in which a nucleic acid-guided nuclease is expressed or maintained, wherein the nucleic acid-guided nuclease is encoded on the vector, a second vector, on the genome of cells of the second population of cells, or otherwise introduced into the cell, resulting in DNA cleavage and incorporation of the editing cassette; (c) obtaining viable cells. Such a method can optionally further comprise (d) sequencing the target DNA molecule in at least one cell of the second population of cells to identify the mutation of at least one codon.
A method for genome editing can comprise: (a) introducing a vector that encodes at least one editing cassette comprising a PAM mutation as disclosed herein and at least one guide nucleic acid into a first population of cells, thereby producing a second population of cells comprising the vector; (b) maintaining the second population of cells under conditions in which nucleic acid-guided nuclease is expressed or maintained, wherein the nucleic acid-guided nuclease is encoded on the vector, a second vector, on the genome of cells of the second population of cells, or otherwise introduced into the cell, resulting in DNA cleavage, incorporation of the editing cassette, and death of cells of the second population of cells that do not comprise the PAM mutation, whereas cells of the second population of cells that comprise the PAM mutation are viable; (c) obtaining viable cells. Such a method can optionally further comprise (d) sequencing the target DNA in at least one cell of the second population of cells to identify the mutation of at least one codon.
Method for trackable genome editing can comprise: (a) introducing a vector that encodes at least one editing cassette, at least one recorder cassette, and at least two gRNA into a first population of cells, thereby producing a second population of cells comprising the vector; (b) maintaining the second population of cells under conditions in which a nucleic acid-guided nuclease is expressed or maintained, wherein the nucleic acid-guided nuclease is encoded on the vector, a second vector, on the genome of cells of the second population of cells, or otherwise introduced into the cell, resulting in DNA cleavage and incorporation of the editing and recorder cassettes; (c) obtaining viable cells. Such a method can optionally further comprise (d) sequencing the recorder sequence of the target DNA molecule in at least one cell of the second population of cells to identify the mutation of at least one codon.
In some examples where the trackable plasmid comprises an editing cassette designed to silence a PAM site, a method for trackable genome editing can comprise: (a) introducing a vector that encodes at least one editing cassette, a recorder cassette, and at least two gRNA into a first population of cells, thereby producing a second population of cells comprising the vector; (b) maintaining the second population of cells under conditions in which a nucleic acid-guided nuclease is expressed or maintained, wherein the nucleic acid-guided nuclease is encoded on the vector, a second vector, on the genome of cells of the second population of cells, or otherwise introduced into the cell, resulting in DNA cleavage, incorporation of the editing cassette and recorder cassette, and death of cells of the second population of cells that do not comprise the PAM mutation, whereas cells of the second population of cells that comprise the PAM mutation are viable; and (c) obtaining viable cells. Such a method can optionally further comprise (d) sequencing the recorder sequence of the target DNA in at least one cell of the second population of cells to identify the mutation of at least one codon. Such methods can also further comprise a recorder cassette comprising a second PAM mutation, such that both PAMs must be silences by the editing cassette PAM mutation and recorder cassette PAM mutation in order to escape cell death.
In some examples transformation efficiency is determined by using a non-targeting guide nucleic acid control, which allows for validation of the recombineering procedure and CFU/ng calculations. In some cases, absolute efficient is obtained by counting the total number of colonies on each transformation plate, for example, by counting both red and white colonies from a galK control. In some examples, relative efficiency is calculated by the total number of successful transformants (for example, white colonies) out of all colonies from a control (for example, galK control).
The methods of the disclosure can provide, for example, greater than 1000× improvements in the efficiency, scale, cost of generating a combinatorial library, and/or precision of such library generation.
The methods of the disclosure can provide, for example, greater than: 10×, 50×, 100×, 200×, 300×, 400×, 500×, 600×, 700×, 800×, 900×, 1000×, 1100×, 1200×, 1300×, 1400×, 1500×, 1600×, 1700×, 1800×, 1900×, 2000×, or greater improvements in the efficiency of generating genomic or combinatorial libraries.
The methods of the disclosure can provide, for example, greater than: 10×, 50×, 100×, 200×, 300×, 400×, 500×, 600×, 700×, 800×, 900×, 1000×, 1100×, 1200×, 1300×, 1400×, 1500×, 1600×, 1700×, 1800×, 1900×, 2000×, or greater improvements in the scale of generating genomic or combinatorial libraries.
The methods of the disclosure can provide, for example, greater than: 10×, 50×, 100×, 200×, 300×, 400×, 500×, 600×, 700×, 800×, 900×, 1000×, 1100×, 1200×, 1300×, 1400×, 1500×, 1600×, 1700×, 1800×, 1900×, 2000×, or greater decrease in the cost of generating genomic or combinatorial libraries.
The methods of the disclosure can provide, for example, greater than: 10×, 50×, 100×, 200×, 300×, 400×, 500×, 600×, 700×, 800×, 900×, 1000×, 1100×, 1200×, 1300×, 1400×, 1500×, 1600×, 1700×, 1800×, 1900×, 2000×, or greater improvements in the precision of genomic or combinatorial library generation.
Recursive Tracking for Combinatorial Engineering Disclosed herein are methods and compositions for iterative rounds of engineering. Disclosed herein are recursive engineering strategies that allow implementation of trackable engineering at the single cell level through several serial engineering cycles (e.g., FIG. 3D or FIG. 6). These disclosed methods and compositions can enable search-based technologies that can effectively construct and explore complex genotypic space. The terms recursive and iterative can be used interchangeably.
Combinatorial engineering methods can comprise multiple rounds of engineering. Methods disclosed herein can comprise 2 or more rounds of engineering. For example, a method can comprise 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 20, 25, 30, or more than 30 rounds of engineering.
In some examples, during each round of engineering a new recorder sequence, such as a barcode, is incorporated at the same or nearby locus in a target site (e.g., FIG. 3D, green bars or FIG. 6, black bars) such that following multiple engineering cycles to construct combinatorial diversity throughout the genome (e.g., FIG. 3E, green bars or FIG. 6, grey bars) a PCR, or similar reaction, of the recording locus can be used to reconstruct each combinatorial genotype or to confirm that the engineered edit from each round has been incorporated into the target site.
Disclosed herein are methods for selecting for successive rounds of engineering. Selection can occur by a PAM mutation incorporated by an editing cassette. Selection can occur by a PAM mutation incorporated by a recorder cassette. Selection can occur using a screenable, selectable, or counter-selectable marker. Selection can occur by targeting a site for editing or recording that was incorporated by a prior round of engineering, thereby selecting for variants that successfully incorporated edits and recorder sequences from both rounds or all prior rounds of engineering.
Quantitation of these genotypes can be used for understanding combinatorial mutational effects on large populations and investigation of important biological phenomena such as epistasis.
Serial editing and combinatorial tracking can be implemented using recursive vector systems as disclosed herein. These recursive vector systems can be used to move rapidly through the transformation procedure (e.g., FIG. 7A). In some examples, these systems consist of two or more plasmids containing orthogonal replication origins, antibiotic markers, and gRNAs. The gRNA in each vector can be designed to target one of the other resistance markers for destruction by nucleic acid-guided nuclease-mediated cleavage. These systems can be used, in some examples, to perform transformations in which the antibiotic selection pressure is switched to remove the previous plasmid and drive enrichment of the next round of engineered genomes. Two or more passages through the transformation loop can be performed, or in other words, multiple rounds of engineering can be performed. Introducing the requisite recording cassettes and editing cassettes into recursive vectors as disclosed herein can be used for simultaneous genome editing and plasmid curing in each transformation step with high efficiencies.
In some examples, the recursive vector system disclosed herein comprises 2, 3, 4, 5, 6, 7, 8, 9, 10, or more than 10 unique plasmids. In some examples, the recursive vector system can use a particular plasmid more than once as long as a distinct plasmid is used in the previous round and in the subsequent round.
Recursive methods and compositions disclosed herein can be used to restore function to a selectable or screenable element in a targeted genome or plasmid. The selectable or screenable element can include an antibiotic resistance gene, a fluorescent gene, a unique DNA sequence or watermark, or other known reporter, screenable, or selectable gene. In some examples, each successive round of engineering can incorporate a fragment of the selectable or screenable element, such that at the end of the engineering rounds, the entire selectable or screenable element has been incorporated into the target genome or plasmid. In such examples, only those genome or plasmids, which have successfully incorporated all of the fragments, and therefore all of the desired corresponding mutations, can be selected or screened for. In this way, the selected or screened cells will be enriched for those that have incorporated the edits from each and every iterative round of engineering.
Recursive methods can be used to switch a selectable or screenable marker between an on and an off position, or between an off and an on position, with each successive round of engineering. Using such a method allows conservation of available selectable or screenable markers by requiring, for example, the use of only one screenable or selectable marker. Furthermore, short regulatory sequence or start codon or non-start codons can be used to turn the screenable or selectable marker on and off. Such short sequences can easily fit within a cassette or polynucleotide, such as a synthesized cassette.
One or more rounds of engineering can be performed using the methods and compositions disclosed herein. In some examples, each round of engineering is used to incorporate an edit unique from that of previous rounds. Each round of engineering can incorporate a unique recording sequence. Each round of engineering can result in removal or curing of the CREATE plasmid used in the previous round of engineering. In some examples, successful incorporation of the recording sequence of each round of engineering results in a complete and functional screenable or selectable marker or unique sequence combination.
Unique recorder cassettes comprising recording sequences such as barcodes or screenable or selectable markers can be inserted with each round of engineering, thereby generating a recorder sequence that is indicative of the combination of edits or engineering steps performed. Successive recording sequences can be inserted adjacent to one another. Successive recording sequences can be inserted within proximity to one another. Successive sequences can be inserted at a distance from one another.
Successive sequences can be inserted at a distance from one another. For example, successive recorder sequences can be inserted and separated by 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, or greater than 100 bp. In some examples, successive recorder sequences are separated by about 10, 50, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, 1000, 1100, 1200, 1300, 1400, 1500, or greater than 1500 bp.
Successive recorder sequences can be separated by any desired number of base pairs and can be dependent and limited on the number of successive recorder sequences to be inserted, the size of the target nucleic acid or target genomes, and/or the design of the desired final recorder sequence. For example, if the compiled recorder sequence is a functional screenable or selectable marker, than the successive recording sequences can be inserted within proximity and within the same reading frame from one another. If the compiled recorder sequence is a unique set of barcodes to be identified by sequencing and have no coding sequence element, then the successive recorder sequences can be inserted with any desired number of base pairs separating them. In these cases, the separation distance can be dependent on the sequencing technology to be used and the read length limit.
In some examples, a recorder cassette comprises a landing site to be used as a target site for the recorder cassette of the next round of engineering. By using such a method, successive rounds of recorder cassettes can only be introduced into the target site if the recorder cassette from the previous round was successfully incorporated, thereby providing the target site for the present engineering round (e.g., FIG. 28).
Guide Nucleic Acid A guide nucleic acid can complex with a compatible nucleic acid-guided nuclease and can hybridize with a target sequence, thereby directing the nuclease to the target sequence. A subject nucleic acid-guided nuclease capable of complexing with a guide nucleic acid can be referred to as a nucleic acid-guided nuclease that is compatible with the guide nucleic acid. Likewise, a guide nucleic acid capable of complexing with a nucleic acid-guided nuclease can be referred to as a guide nucleic acid that is compatible with the nucleic acid-guided nucleases.
A guide nucleic acid can be DNA. A guide nucleic acid can be RNA. A guide nucleic acid can comprise both DNA and RNA. A guide nucleic acid can comprise modified of non-naturally occurring nucleotides. In cases where the guide nucleic acid comprises RNA, the RNA guide nucleic acid can be encoded by a DNA sequence on a polynucleotide molecule such as a plasmid, linear construct, or editing cassette as disclosed herein.
A guide nucleic acid can comprise a guide sequence. A guide sequence is a polynucleotide sequence having sufficient complementarity with a target polynucleotide sequence to hybridize with the target sequence and direct sequence-specific binding of a complexed nucleic acid-guided nuclease to the target sequence. The degree of complementarity between a guide sequence and its corresponding target sequence, when optimally aligned using a suitable alignment algorithm, is about or more than about 50%, 60%, 75%, 80%, 85%, 90%, 95%, 97.5%, 99%, or more. Optimal alignment may be determined with the use of any suitable algorithm for aligning sequences. In some embodiments, a guide sequence is about or more than about 5, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 35, 40, 45, 50, 75, or more nucleotides in length. In some embodiments, a guide sequence is less than about 75, 50, 45, 40, 35, 30, 25, 20 nucleotides in length. Preferably the guide sequence is 10-30 nucleotides long. The guide sequence can be 15-20 nucleotides in length. The guide sequence can be 15 nucleotides in length. The guide sequence can be 16 nucleotides in length. The guide sequence can be 17 nucleotides in length. The guide sequence can be 18 nucleotides in length. The guide sequence can be 19 nucleotides in length. The guide sequence can be 20 nucleotides in length.
A guide nucleic acid can comprise a scaffold sequence. In general, a “scaffold sequence” includes any sequence that has sufficient sequence to promote formation of a targetable nuclease complex, wherein the targetable nuclease complex comprises a nucleic acid-guided nuclease and a guide nucleic acid comprising a scaffold sequence and a guide sequence. Sufficient sequence within the scaffold sequence to promote formation of a targetable nuclease complex may include a degree of complementarity along the length of two sequence regions within the scaffold sequence, such as one or two sequence regions involved in forming a secondary structure. In some cases, the one or two sequence regions are comprised or encoded on the same polynucleotide. In some cases, the one or two sequence regions are comprised or encoded on separate polynucleotides. Optimal alignment may be determined by any suitable alignment algorithm, and may further account for secondary structures, such as self-complementarity within either the one or two sequence regions. In some embodiments, the degree of complementarity between the one or two sequence regions along the length of the shorter of the two when optimally aligned is about or more than about 25%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 97.5%, 99%, or higher. In some embodiments, at least one of the two sequence regions is about or more than about 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 40, 50, or more nucleotides in length.
A scaffold sequence of a subject guide nucleic acid can comprise a secondary structure. A secondary structure can comprise a pseudoknot region. In some example, the compatibility of a guide nucleic acid and nucleic acid-guided nuclease is at least partially determined by sequence within or adjacent to a pseudoknot region of the guide RNA. In some cases, binding kinetics of a guide nucleic acid to a nucleic acid-guided nuclease is determined in part by secondary structures within the scaffold sequence. In some cases, binding kinetics of a guide nucleic acid to a nucleic acid-guided nuclease is determined in part by nucleic acid sequence with the scaffold sequence.
In aspects of the invention the terms “guide nucleic acid” refers to a polynucleotide comprising 1) a guide sequence capable of hybridizing to a target sequence and 2) a scaffold sequence capable of interacting with or complexing with an nucleic acid-guided nuclease as described herein.
A guide nucleic acid can be compatible with a nucleic acid-guided nuclease when the two elements can form a functional targetable nuclease complex capable of cleaving a target sequence. Often, a compatible scaffold sequence for a compatible guide nucleic acid can be found by scanning sequences adjacent to a native nucleic acid-guided nuclease loci. In other words, native nucleic acid-guided nucleases can be encoded on a genome within proximity to a corresponding compatible guide nucleic acid or scaffold sequence.
Nucleic acid-guided nucleases can be compatible with guide nucleic acids that are not found within the nucleases endogenous host. Such orthogonal guide nucleic acids can be determined by empirical testing. Orthogonal guide nucleic acids can come from different bacterial species or be synthetic or otherwise engineered to be non-naturally occurring.
Orthogonal guide nucleic acids that are compatible with a common nucleic acid-guided nuclease can comprise one or more common features. Common features can include sequence outside a pseudoknot region. Common features can include a pseudoknot region. Common features can include a primary sequence or secondary structure.
A guide nucleic acid can be engineered to target a desired target sequence by altering the guide sequence such that the guide sequence is complementary to the target sequence, thereby allowing hybridization between the guide sequence and the target sequence. A guide nucleic acid with an engineered guide sequence can be referred to as an engineered guide nucleic acid. Engineered guide nucleic acids are often non-naturally occurring and are not found in nature.
More Methods Disclosed herein are methods for genome engineering that employ a nuclease, such as a nucleic acid-guided nuclease to perform directed genome evolution/produce changes (deletions, substitutions, additions) in a target sequence, such as DNA or RNA, for example, genomic DNA or episomal DNA. Suitable nucleases can include, for example, RNA-guided nucleases such as Cas9, Cpf1, MAD2, or MAD7, DNA-guided nucleases such as Argonaute, or other nucleases such as zinc-finger nucleases, TALENs, or meganucleases. Nuclease genes can be obtained from any source, such as from a bacterium, archaea, prokaryote, eukaryote, or virus. For example, a Cas9 gene can be obtained from a bacterium harboring the corresponding Type II CRISPR system, such as the bacterium S. pyogenes (SEQ ID NO: 110). The nucleic acid sequence and/or amino acid sequence of the nuclease may be mutated, relative to the sequence of a naturally occurring nuclease. A mutation can be, for example, one or more insertions, deletions, substitutions or any combination of two or three of the foregoing. In some cases, the resulting mutated nuclease can have enhanced or reduced nuclease activity relative to the naturally occurring nuclease. In some cases, the resulting mutated nuclease can have no nuclease activity relative to the naturally occurring nuclease.
Methods for nucleic acid-guided nuclease-mediated genome editing are provided herein. Some disclosed methods can include a two-stage construction process which relies on generation of cassette libraries that incorporate directed mutations from an editing cassettes directly into a genome, episomal nucleic acid molecule, or isolated nucleic acid molecule. In some examples, during the first stage of cassette library construction, rationally designed editing cassettes can be cotransformed into cells with a guide nucleic acid (e.g., guide RNA) that hybridizes to or targets a target DNA sequence. In some examples, the guide nucleic acid is introduced as an RNA molecule, or encoded on a DNA molecule.
Editing cassettes can be designed such that they couple deletion or mutation of a PAM site with the mutation of one or more desired codons or nucleic acid residues in the adjacent nucleic acid sequence. The deleted or mutated PAM site, in some cases, can no longer be recognized by the chosen nucleic acid-guided nuclease. In some examples, at least one PAM or more than one PAM can be deleted or mutated, such as two, three, four, or more PAMs.
Methods disclosed herein can enable generation of an entire cassette library in a single transformation. The cassette library can be retrieved, in some cases, by amplification of the recombinant chromosomes, e.g. by a PCR reaction, using a synthetic feature or priming site from the editing cassettes. In some examples, a second PAM deletion or mutation is simultaneously incorporated. This approach can covalently couple the codon-targeted mutations directly to a PAM deletion.
In some examples, there is a second stage to construction of cassette libraries. During the second stage the PCR amplified cassette libraries carrying the destination PAM deletion/mutation and the targeted mutations, such as a desired mutation of one or more nucleotides, such as one or more nucleotides in one or more codons, can be co-transformed into naive cells. The cells can be eukaryotic cell, archaeal cell, or prokaryotic cells. The cassette libraries can be co-transformed with a guide nucleic acid or plasmid encoding the same to generate a population of cells that express a rationally designed protein library. The libraries can be co-transformed with a guide nucleic acid such as a gRNA, chimeric gRNA, split gRNA, or a crRNA and trRNA set. The cassette library can comprise a plurality of cassettes wherein each cassette comprises an editing cassette and guide nucleic acid. The cassette library can comprise a plurality of cassettes wherein each cassette comprises an editing cassette, recorder cassettes and two guide nucleic acids.
In some targetable nuclease systems, the guide nucleic acid can guide selection of a target sequence. As used herein, a target sequence refers to any locus in vitro or in in vivo, or in the nucleic acid of a cell or population of cells in which a mutation of at least one nucleotide, such as a mutation of at least one nucleotide in at least one codon, is desired. The target sequence can be, for example, a genomic locus, target genomic sequence, or extrachromosomal locus. The guide nucleic acid can be expressed as a DNA molecule, referred to as a guide DNA, or as a RNA molecule, referred to as a guide RNA. A guide nucleic acid can comprise a guide sequence, that is complementary to a region of the target region. A guide nucleic acid can comprise a scaffold sequence that can interact with a compatible nucleic acid-guided nuclease, and can optionally form a secondary structure. A guide nucleic acid can functions to recruit a nucleic acid-guided nuclease to the target site. A guide sequence can be complementary to a region upstream of the target site. A guide sequence can be complementary to at least a portion of the target site. A guide sequence can be completely complementary (100% complementary) to the target site or include one or more mismatches, provided that it is sufficiently complementary to the target site to specifically hybridize/guide and recruit the nuclease. Suitable nucleic acid guided nuclease include, as non-limiting examples, CRISPR nucleases, Cas nucleases, such as Cas9 or Cpf1, MAD2, and MAD7.
In some CRISPR systems, the CRISPR RNA (crRNA or spacer-containing RNA) and trans-activating CRISPR RNA (tracrRNA or trRNA) can guide selection of a target sequence. As used herein, a target sequence refers to any locus in vitro or in in vivo, or in the nucleic acid of a cell or population of cells in which a mutation of at least one nucleotide, such as a mutation of at least one nucleotide in at least one codon, is desired. The target sequence can be, for example, a genomic locus, target genomic sequence, or extrachromosomal locus. The tracrRNA and crRNA can be expressed as a single, chimeric RNA molecule, referred to as a single-guide RNA, guide RNA, or gRNA. The nucleic acid sequence of the gRNA comprises a first nucleic acid sequence, also referred to as a first region, that is complementary to a region of the target region and a second nucleic acid sequence, also referred to a second region, that forms a stem loop structure and functions to recruit a CRISPR nuclease to the target region. The first region of the gRNA can be complementary to a region upstream of the target genomic sequence. The first region of the gRNA can be complementary to at least a portion of the target region. The first region of the gRNA can be completely complementary (100% complementary) to the target genomic sequence or include one or more mismatches, provided that it is sufficiently complementary to the target genomic sequence to specifically hybridize/guide and recruit a CRISPR nuclease, such as Cas9 or Cpf1.
A guide sequence or first region of the gRNA can be at least 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or at least 30 nucleotides in length. The guide sequence or first region of the gRNA can be at least 20 nucleotides in length.
A stem loop structure that can be formed by the scaffold sequence or second nucleic acid sequence of a gRNA can be at least 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 7, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, or 100 nucleotides in length. A stem loop structure can be from 80 to 90 or 82 to 85 nucleotides in length. A scaffold sequence or second region of the gRNA that forms a stem loop structure can be 83 nucleotides in length.
A guide nucleic acid of a cassette that is introduced into a first cell using the methods disclosed herein can be the same as the guide nucleic acid of a second cassette that is introduced into a second cell. More than one guide nucleic acid can be introduced into the population of first cells and/or the population of second cells. The more than one guide nucleic acids can comprise guide sequences that are complementary to more than one target region.
Methods disclosed herein can comprise using oligonucleotides. Such oligonucleotides can be obtained or derived from many sources. For example, an oligonucleotide can be derived from a nucleic acid library that has been diversified by nonhomologous random recombination (NRR); such a library is referred to as an NRR library. An oligonucleotide can be synthesized, for example by array-based synthesis or other known chemical synthesis method. The length of an oligonucleotide can be dependent on the method used in obtaining the oligonucleotide. An oligonucleotide can be approximately 50-200 nucleotides, 75-150 nucleotides, or between 80-120 nucleotides in length. An oligonucleotide can be about 10, 20, 30, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, or more nucleotides in length, including any integer, for example, 51, 52, 53, 54, 201, 202, etc. An oligonucleotide can be about 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1250, 1500, 1750, 2000, or more nucleotides in length, including any integer, for example, 101, 203, 1001, 2001, 2010, etc.
Oligonucleotides and/or other nucleic acid molecules can be combined or assembled to generate a cassette. Such a cassette can comprise (a) a region that is homologous to a target region of the nucleic acid of the cell and includes a desired mutation of at least one nucleotide or one codon relative to the target region, and (b) a protospacer adjacent motif (PAM) mutation. The PAM mutation can be any insertion, deletion or substitution of one or more nucleotides that mutates the sequence of the PAM such that it is no longer recognized by a nucleic acid-guided nuclease system or CRISPR nuclease system. A cell that comprises such a PAM mutation may be said to be “immune” to nuclease-mediated killing. The desired mutation relative to the sequence of the target region can be an insertion, deletion, and/or substitution of one or more nucleotides. In some examples, the insertion, deletion, and/or substitution of one or more nucleotides is in at least one codon of the target region. Alternatively, the cassette can be synthesized in a single synthesis, comprising (a) a region that is homologous to a target region of the nucleic acid of the cell and includes a desired mutation of at least one nucleotide or one codon relative to the target region, (b) a protospacer adjacent motif (PAM) mutation, and optionally (c) a region that is homologous to a second target region of the nucleic acid of the cell and includes a recorder sequence.
The methods disclosed herein can be applied to any target nucleic acid molecule of interest, from any prokaryote including bacteria and archaea, or any eukaryote, including yeast, mammalian, and human genes, or any viral particle. The nucleic acid module can be a non-coding nucleic acid sequence, gene, genome, chromosome, plasmid, episomal nucleic acid molecule, artificial chromosome, synthetic chromosome, or viral nucleic acid.
Methods for assessing recovery efficiency of donor strain libraries are disclosed herein. Recovery efficiency can be verified based on the presence of a PCR product or on changes in amplicon or PCR product sizes or sequence obtained with primers directed at the selected target locus. Primers can be designed to hybridize with endogenous sequences or heterologous sequences contained on the donor nucleic acid molecule. For example, the PCR primer can be designed to hybridize to a heterologous sequence such that PCR will only be possible if the donor nucleic acid is incorporated. Sequencing of PCR products from the recovered libraries indicates the heterologous sequence or synthetic priming site from the dsDNA cassettes or donor sequences can be incorporated with about 90-100% efficiency. In other examples, the efficiency can be about 5%, 10% 20%, 30%, 40%, 50%, 60%, 70%, 75%, 80%, 85%, 90%, 95%, 99% or 100%.
In some cases, the ability to improve final editing efficiencies of the methods disclosed herein can be assessed by carrying out cassette construction in gene deficient strains before transferring to a wild-type donor strain in an effort to prevent loss of mutations during the donor construction phase. Additionally or alternatively, efficiency of the disclosed methods can be assessed by targeting an essential gene. Essential genes can include any gene required for survival or replication of a viral particle, cell, or organism. In some examples, essential genes include dxs, metA, and folA. Essential genes have been effectively targeted using guide nucleic acid design strategies described. Other suitable essential genes are well known in the art.
Provided herein are method of increasing editing efficiencies by modulating the level of a nucleic acid-guided nuclease. This could be done by using copy control plasmids, such as high copy number plasmids or low copy number plasmids. Low copy number plasmids could be plasmids that can have about 20 or less copies per cell, as opposed to high copy number plasmids that can have about 1000 copies per cell. High copy number plasmids and low copy number plasmids are well known in the art and it is understood that an exact plasmid copy per cell does not need to be known in order to characterize a plasmid as either high or low copy number.
In some cases, the decreasing expression level of a nucleic acid-guided nuclease, such as Cas9, Cpf1, MAD2, or MAD7, can increase transformation, editing, and/or recording efficiencies. In some cases, decreasing expression level of the nucleic acid-guided nuclease is done by expressing the nucleic acid-guided nuclease on a low copy number plasmid.
In some cases, the increasing expression level of a nucleic acid-guided nuclease, such as Cas9, Cpf1, MAD2, or MAD7, can increase transformation, editing, and/or recording efficiencies. In some cases, increasing expression level of the nucleic acid-guided nuclease is done by expressing the nucleic acid-guided nuclease on a high copy number plasmid.
Other methods of modulating the expression level of a protein are also envisioned and are known in the art. Such methods include using a inducible or constitutive promoter, incorporating enhancers or other expression regulatory elements onto an expression plasmid, using RNAi, amiRNAi, or other RNA silencing techniques to modulate transcript level, fusing the protein of interest to a degradation domain, or any other method known in the art.
Provided herein are methods for generating mutant libraries. In some examples, the mutant library can be effectively constructed and retrieved within 1-3 hours post recombineering. In some examples, the mutant library is constructed within 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, or 24 hours post recombineering. In some examples, the mutant library can be retrieved within 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 24, 36, or 48 hours post recombineering and/or post-constructing by recombineering.
Some methods disclosed herein can be used for trackable, precision genome editing. In some examples, methods disclosed herein can achieve high efficiency editing/mutating using a single cassette that encodes both an editing cassette and guide nucleic acid, and optionally a recorder cassette and second guide nucleic acid. Alternatively, a single vector can encode an editing cassette while a guide nucleic acid is provided sequentially or concomitantly. When used with parallel DNA synthesis, such as array-based DNA synthesis, methods disclosed herein can provide single step generation of hundreds or thousands of precision edits/mutations. Mutations can be mapped by sequencing the editing cassette on the vector, rather than by sequencing of the genome or a section of the genome of the cell or organism.
The methods disclosed herein can have broad utility in protein and genome engineering applications, as well as for reconstruction of mutations, such as mutations identified in laboratory evolution experiments. In some examples, the methods and compositions disclosed here can combine an editing cassette, which could include a desired mutation and a PAM mutation, with a gene encoding a guide nucleic acid on a single vector.
In some examples, a trackable mutant library can be generated in a single transformation or single reaction.
Methods disclosed herein can comprise introducing a cassette comprising an editing cassette that includes the desired mutation and the PAM mutation into a cell or population of cells. In some embodiments, the cell into which the cassette or vector is introduced also comprises a nucleic acid-guided nuclease, such as Cas9, Cpf1, MAD2, or MAD7. In some embodiments, a gene or mRNA encoding the nucleic acid-guided nuclease is concomitantly, sequentially, or subsequently introduced into the cell or population of cells. Expression of a targetable nuclease system, including nucleic acid-guided nuclease and a guide nucleic acid, in the cell or cell population can be activated such that the guide nucleic acid recruits the nucleic acid-guided nuclease to the target region, where dsDNA cleavage occurs.
In some examples, without wishing to be bound by any particular theory, the homologous region of an editing cassette complementary to the target sequence mutates the PAM and the one or more codon of the target sequence. Cells of the population of cells that did not integrate the PAM mutation can undergo unedited cell death due to nucleic acid-guided nuclease mediated dsDNA cleavage. In some examples, cells of the population of cells that integrate the PAM mutation do not undergo cell death; they remain viable and are selectively enriched to high abundance. Viable cells can be obtained and can provide a library of trackable or targeted mutations.
In some examples, without wishing to be bound by any particular theory, the homologous region of a recorder cassette complementary to the target sequence mutates the PAM and introduces a barcode into a target sequence. Cells of the population of cells that did not integrate the PAM mutation can undergo unedited cell death due to nucleic acid-guided nuclease mediated dsDNA cleavage. In some examples, cells of the population of cells that integrate the PAM mutation do not undergo cell death; they remain viable and are selectively enriched to high abundance. Viable cells can be obtained and can provide a library of trackable mutations.
A separate vector or mRNA encoding a nucleic acid-guided nuclease can be introduced into the cell or population of cells. Introducing a vector or mRNA into a cell or population of cells can be performed using any method or technique known in the art. For example, vectors can be introduced by standard protocols, such as transformation including chemical transformation and electroporation, transduction and particle bombardment. Additionally or alternatively, mRNA can be introduced by standard protocols, such as transformation as disclosed herein, and/or by techniques involving cell permeable peptides or nanoparticles.
An editing cassette can include (a) a region, which recognizes (hybridizes to) a target region of a nucleic acid in a cell or population of cells, is homologous to the target region of the nucleic acid of the cell and includes a mutation, referred to a desired mutation, of at least one nucleotide that can be in at least one codon relative to the target region, and (b) a protospacer adjacent motif (PAM) mutation. In some examples, the editing cassette also comprises a barcode. The barcode can be a unique barcode or relatively unique such that the corresponding mutation can be identified based on the barcode. The PAM mutation may be any insertion, deletion or substitution of one or more nucleotides that mutates the sequence of the PAM such that the mutated PAM (PAM mutation) is not recognized by a chosen nucleic acid-guided nuclease system. A cell that comprises such as a PAM mutation may be said to be “immune” to nucleic acid-guided nuclease-mediated killing. The desired mutation relative to the sequence of the target region may be an insertion, deletion, and/or substitution of one or more nucleotides and may be at least one codon of the target region. In some embodiments, the distance between the PAM mutation and the desired mutation is at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 40, 50, 60, 70, 80, 90, or 100 nucleotides on the editing cassette In some embodiments, the PAM mutation is located at least 9 nucleotides from the end of the editing cassette. In some embodiments, the desired mutation is located at least 9 nucleotides from the end of the editing cassette.
A desired mutation can be an insertion of a nucleic acid sequence relative to the sequence of the target sequence. The nucleic acid sequence inserted into the target sequence can be of any length. In some embodiments, the nucleic acid sequence inserted is at least 1, 2, 3, 4, 5, 10, 20, 30, 40, 50, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, 1000, 1100, 1200, 1300, 1400, 1500, 1600, 1700, 1800, 1900, or at least 2000 nucleotides in length. In embodiments in which a nucleic acid sequence is inserted into the target sequence, the editing cassette comprises a region that is at least 10, 15, 20, 25, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50 51, 52, 53, 54, 55, 56, 57, 58, 59, or at least 60 nucleotides in length and homologous to the target sequence. The homology arms or homologous region can be about 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, or more nucleotides in length, including any integer therein. The homology arms or homologous region can be over 200 nucleotides in length.
A barcode can be a unique barcode or relatively unique such that the corresponding mutation can be identified based on the barcode. In some examples, the barcode is a non-naturally occurring sequence that is not found in nature. In most examples, the combination of the desired mutation and the barcode within the editing cassette is non-naturally occurring and not found in nature. A barcode can be any number of nucleotides in length. A barcode can be 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, or more than 30 nucleotides in length. In some cases, the barcode is more than 30 nucleotides in length.
An editing cassette or recorder cassette can comprise at least a portion of a gene encoding a guide nucleic acid, and optionally a promoter operable linked to the encoded guide nucleic acid. In some embodiments, the portion of the gene encoding the guide nucleic acid encodes the portion of the guide nucleic acid that is complementary to the target sequence. The portion of the guide nucleic acid that is complementary to the target sequence, or the guide sequence, can be at least 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or at least 30 nucleotides in length. In some embodiments, the guide sequence is 24 nucleotides in length. In some embodiments, the guide sequence is 18 nucleotides in length.
In some embodiments, the editing cassette or recorder cassette further comprises at least two priming sites. The priming sites may be used to amplify the cassette, for example by PCR. In some embodiments, the portion of the guide sequence is used as a priming site.
Editing cassettes or recorder cassettes for use in the described methods can be obtained or derived from many sources. For example, the cassettes can be synthesized, for example by array-based synthesis, multiplex synthesis, multi-parallel synthesis, PCR assembly, in vitro assembly, Gibson assembly, or any other synthesis method known in the art. In some embodiments, the editing cassette or recorder cassette is synthesized, for example by array-based synthesis, multiplex synthesis, multi-parallel synthesis, PCR assembly,—in vitro assembly, Gibson assembly, or any other synthesis method known in the art. The length of the editing cassette or recorder cassette may be dependent on the method used in obtaining said cassette.
An editing cassette can be approximately 50-300 nucleotides, 75-200 nucleotides, or between 80-120 nucleotides in length. In some embodiments, the editing cassette can be any discrete length between 50 nucleotide and 1 Mb.
A recorder cassette can be approximately 50-300 nucleotides, 75-200 nucleotides, or between 80-120 nucleotides in length. In some embodiments, the recorder cassette can be any discrete length between 50 nucleotide and 1 Mb.
Methods disclosed herein can also involve obtaining editing cassettes and recorder cassettes and constructing a trackable plasmid or vector. Methods of constructing a vector will be known to one ordinary skill in the art and may involve ligating the cassettes into a vector backbone. In some examples, plasmid construction occurs by in vitro DNA assembly methods, oligonucleotide assembly, PCR-based assembly, SLIC, CPEC, or other assembly methods well known in the art. In some embodiments, the cassettes or a subset (pool) of the cassettes can be amplified prior to construction of the vector, for example by PCR.
The cell or population of cells comprising a polynucleotide encoding a nucleic acid-guided nuclease can be maintained or cultured under conditions in which the nuclease is expressed. Nucleic acid-guided nuclease expression can be controlled or can be constitutively on. The methods described herein can involve maintaining cells under conditions in which nuclease expression is activated, resulting in production of the nuclease, for example, Cas9, Cpf1, MAD2, or MAD7. Specific conditions under which the nucleic acid-guided nuclease is expressed can depend on factors, such as the nature of the promoter used to regulate expression of the nuclease. Nucleic acid-guided nuclease expression can be induced in the presence of an inducer molecule, such as arabinose. When the cell or population of cells comprising nucleic acid-guided nuclease encoding DNA are in the presence of the inducer molecule, expression of the nuclease can occur. CRISPR-nuclease expression can be repressed in the presence of a repressor molecule. When the cell or population of cells comprising nucleic acid-guided nuclease encoding DNA are in the absence of a molecule that represses expression of the nuclease, expression of the nuclease can occur.
Cells or the population of cells that remain viable can be obtained or separated from the cells that undergo unedited cell death as a result of nucleic acid-guided nuclease-mediated killing; this can be done, for example, by spreading the population of cells on culture surface, allowing growth of the viable cells, which are then available for assessment.
Disclosed herein are methods for the identification of the mutation without the need to sequence the genome or large portions of the genome of the cell. The methods can involve sequencing of the editing cassette, recorder cassette, or barcode to identify the mutation of one of more codon. Sequencing of the editing cassette can be performed as a component of the vector or after its separation from the vector and, optionally, amplification. Sequencing can be performed using any sequencing method known in the art, such as by Sanger sequencing or next-generation sequencing methods.
Some methods described herein can be carried out in any type of cell in which a targetable nuclease system can function, or target and cleave DNA, including prokaryotic and eukaryotic cells. In some embodiments, the cell is a bacterial cell, such as Escherichia spp., e.g., E. coli. In other embodiments, the cell is a fungal cell, such as a yeast cell, e.g., Saccharomyces spp. In other embodiments, the cell is an algal cell, a plant cell, an insect cell, or a mammalian cell, including a human cell.
A “vector” is any of a variety of nucleic acids that comprise a desired sequence or sequences to be delivered to or expressed in a cell. A desired sequence can be included in a vector, such as by restriction and ligation or by recombination or assembly methods know in the art. Vectors are typically composed of DNA, although RNA vectors are also available. Vectors include, but are not limited to plasmids, fosmids, phagemids, virus genomes, artificial chromosomes, and synthetic nucleic acid molecules.
Vectors useful in the methods disclosed herein can comprise at least one editing cassette as described herein, at least one gene encoding a gRNA, and optionally a promoter and/or a barcode. More than one editing cassette can be included on the vector, for example 2, 3, 4, 5, 6, 7, 8, 9, 10 or more editing cassettes. The more than one editing cassettes can be designed to target different target regions, for example, there could be different editing cassettes, each of which contains at least one region homologous with a different target region. In other examples, each editing cassette target the same target region while each editing cassette comprises a different desired mutation relative to the target region. In other examples, the plurality of editing cassettes can comprise a combination of editing cassettes targeting the same target region and editing cassettes targeting different target regions. Each editing cassette can comprise an identifying barcode. Alternatively or additionally, the vector can include one or more genes encoding more than one gRNA, e.g., 2, 3, 4, 5, 6, 7, 8, 9, 10 or more gRNAs. The more than one gRNAs can contain regions that are complementary to a portion of different target regions, for example, if there are different gRNAs, each of which can be complementary to a portion of a different target region. In other examples, the more than one gRNA can each target the same target region. In other examples, the more than one gRNA can be a combination of gRNAs targeting the same and different target regions.
A cassette comprising a gene encoding a portion of a guide nucleic acid, can be ligated or assembled into a vector that encodes another portion of a guide nucleic acid. Upon ligation or assembly, the portion of the guide nucleic acid from the cassette and the other portion of the guide nucleic acid can form a functional guide nucleic acid. A promoter and a gene encoding a guide nucleic acid can be operably linked.
In some embodiments, the methods involve introduction of a second vector encoding a nucleic acid-guided nuclease, such as Cas9, Cpf1, MAD2, or MAD7. The vector may further comprise one or more promoters operably linked to a gene encoding the nucleic acid-guided nuclease.
As used herein, “operably” linked can mean the promoter affects or regulates transcription of the DNA encoding a gene, such as the gene encoding the gRNA or the gene encoding a CRISPR nuclease.
A promoter can be a native promoter such as a promoter present in the cell into which the vector is introduced. A promoter can be an inducible or repressible promoter, for example, the promoter can be regulated allowing for inducible or repressible transcription of a gene, such as the gene encoding the guide nucleic acid or the gene encoding a nucleic acid-guided nuclease. Such promoters that are regulated by the presence or absence of a molecule can be referred to as an inducer or a repressor, respectively. The nature of the promoter needed for expression of the guide nucleic acid or nucleic acid-guided nuclease can vary based on the species or cell type and can be recognized by one of ordinary skill in the art.
A separate vector encoding a nucleic acid-guided nuclease can be introduced into a cell or population of cells before or at the same time as introduction of a trackable plasmid as disclosed herein. The gene encoding a nucleic acid-guided nuclease can be integrated into the genome of the cell or population of cells, or the gene can be maintained episomally. The nucleic acid-guided nuclease-encoding DNA can be integrated into the cellular genome before introduction of the trackable plasmid, or after introduction of the trackable plasmid. In some examples, a nucleic acid molecule, such as DNA-encoding a nucleic acid-guided nuclease, can be expressed from DNA integrated into the genome. In some embodiments, a gene encoding Cas9, Cpf1, MAD2, or MAD7 is integrated into the genome of the cell.
Vectors or cassettes useful in the methods described herein can further comprise two or more priming sites. In some embodiments, the presence of flanking priming sites allows amplification of the vector or cassette.
In some embodiments, a cassette or vector encodes a nucleic acid-guided nuclease comprising one or more nuclear localization sequences (NLSs), such as about or more than about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or more NLSs. In some embodiments, the engineered nuclease comprises about or more than about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or more NLSs at or near the amino-terminus, about or more than about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or more NLSs at or near the carboxy-terminus, or a combination of these (e.g. one or more NLS at the amino-terminus and one or more NLS at the carboxy terminus). When more than one NLS is present, each may be selected independently of the others, such that a single NLS may be present in more than one copy and/or in combination with one or more other NLSs present in one or more copies. In a preferred embodiment of the invention, the engineered nuclease comprises at most 6 NLSs. In some embodiments, an NLS is considered near the N- or C-terminus when the nearest amino acid of the NLS is within about 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 40, 50, or more amino acids along the polypeptide chain from the N- or C-terminus. Non-limiting examples of NLSs include an NLS sequence derived from: the NLS of the SV40 virus large T-antigen, having the amino acid sequence PKKKRKV (SEQ ID NO: 111); the NLS from nucleoplasmin (e.g. the nucleoplasmin bipartite NLS with the sequence KRPAATKKAGQAKKKK (SEQ ID NO:112)); the c-myc NLS having the amino acid sequence PAAKRVKLD (SEQ ID NO:113) or RQRRNELKRSP (SEQ ID NO:114); the hRNPA1 M9 NLS having the sequence NQSSNFGPMKGGNFGGRSSGPYGGGGQYFAKPRNQGGY (SEQ ID NO: 115); the sequence RMRIZFKNKGKDTAELRRRRVEVSVELRKAKKDEQILKRRNV (SEQ ID NO: 116) of the IBB domain from importin-alpha; the sequences VSRKRPRP (SEQ ID NO:117) and PPKKARED (SEQ ID NO:11 of the myoma T protein; the sequence PQPKKKPL (SEQ ID NO:119) of human p53; the sequence SALIKKKKKMAP (SEQ ID NO:120) of mouse c-abl IV; the sequences DRLRR (SEQ ID NO:121) and PKQKKRK (SEQ ID NO:122) of the influenza virus NS1; the sequence RKLKKKIKKL (SEQ ID NO:123) of the Hepatitis virus delta antigen; the sequence REKKKFLKRR (SEQ ID NO: 124) of the mouse M×1 protein; the sequence KRKGDEVDGVDEVAKKKSKK (SEQ ID NO: 125) of the human poly(ADP-ribose) polymerase; and the sequence RKCLQAGMNLEARKTKK (SEQ ID NO: 126) of the steroid hormone receptors (human) glucocorticoid.
In general, the one or more NLSs are of sufficient strength to drive accumulation of the nucleic acid-guided nuclease in a detectable amount in the nucleus of a eukaryotic cell. In general, strength of nuclear localization activity may derive from the number of NLSs, the particular NLS(s) used, or a combination of these factors. Detection of accumulation in the nucleus may be performed by any suitable technique. For example, a detectable marker may be fused to the nucleic acid-guided nuclease, such that location within a cell may be visualized, such as in combination with a means for detecting the location of the nucleus (e.g. a stain specific for the nucleus such as DAPI). Cell nuclei may also be isolated from cells, the contents of which may then be analyzed by any suitable process for detecting protein, such as immunohistochemistry, Western blot, or enzyme activity assay. Accumulation in the nucleus may also be determined indirectly, such as by an assay for the effect of the nucleic acid-guided nuclease complex formation (e.g. assay for DNA cleavage or mutation at the target sequence, or assay for altered gene expression activity affected by targetable nuclease complex formation and/or nucleic acid-guided nuclease activity), as compared to a control not exposed to the nucleic acid-guided nuclease or targetable nuclease complex, or exposed to a nucleic acid-guided nuclease lacking the one or more NLSs.
ProSAR Methods disclosed herein are capable of engineering a few to hundreds of genetic sequence or proteins simultaneously. These methods can permit one to map in a single experiment many or all possible residue changes over a collection of desired proteins onto a trait of interest, as part of individual proteins of interest or as part of a pathway. This approach can be used at least for the following by mapping i) any number of residue changes for any number of proteins of interest in a specific biochemical pathway or that catalyze similar reactions or ii) any number of residues in the regulatory sites of any number of proteins or interest with a specific regulon or iii) any number of residues of a biological agent used to treat a health condition.
In some embodiments, methods described herein include identifying genetic variations of one or more target genes that affect any number or residues, such as one or more, or all residues of one or more target proteins. In accordance with these embodiments, compositions and methods disclosed herein permit parallel analysis of two or more target proteins or proteins that contribute to a trait. Parallel analysis of multiple proteins by a single experiment described can facilitate identification, modification and design of superior systems for example for producing a eukaryotic or prokaryotic byproduct, producing a eukaryotic byproduct, for example, a biological agent such as a growth factor or antibody, in a prokaryotic organism and the like. Relevant biologics used in analysis and treatment of disease can be produced in these genetically engineered environments that could reduce production time, increase quality all while reducing costs to the manufacturers and the consumers.
Some embodiments disclosed herein comprise constructs of use for studying genetic variations of a gene or gene segment wherein the gene or gene segment is capable of generating a protein. A construct can be generated for any number of residues, such as one, two, more than two, or all residue modifications of a target protein that is linked to a trackable agent such as a barcode. A barcode indicative of a genetic variation of a gene of a target protein can be located outside of the open reading frame of the gene. In some embodiments such a barcode can be located many hundreds or thousands of bases away from the gene. It is contemplated herein that these methods can be performed in vivo. In some examples, such a construct comprises a trackable polynucleic acid or plasmid as disclosed herein.
Constructs described herein can be used to compile a comprehensive library of genetic variations encompassing all residue changes of one target protein, more than one target protein or target proteins that contribute to a trait. In certain embodiments, libraries disclosed herein can be used to select proteins with improved qualities to create an improved single or multiple protein system for example for producing a byproduct, such as a chemical, biofuels, biological agent, pharmaceutical agent, or for biomass, or biologic compared to a non-selective system.
Protein Sequence Activity Relationship (ProSAR) Mapping Understanding the relationship between a protein's amino acid structure and its overall function continues to be of great practical, clinical, and scientific significance for biologists and engineers. Directed evolution can be a powerful engineering and discovery tool, but the random and often combinatorial nature of mutations makes their individual impacts difficult to quantify and thus challenges further engineering. More systematic analysis of contributions of individual residues or saturation mutagenesis remains labor- and time-intensive for entire proteins and simply is not possible on reasonable timescales for multiple proteins in parallel, such as metabolic pathways or multi-protein complexes, using standard methods.
Provided herein are methods which can be used to rapidly and efficiently examine the roles of some or all genes in a viral, microbial, or eukaryotic genome using mixtures of barcoded oligonucleotides. In some embodiments, these compositions and methods can be used to develop a powerful new technology for comprehensively mapping protein structure-activity relationships (ProSAR).
Using methods and compositions disclosed herein, multiplex cassette synthesis can be combined with recombineering, to create mutant libraries of specifically designed and barcoded mutations along one or more genes of interest in parallel. Screens and/or selections followed by high-throughput sequencing and/or barcode microarray methods can allow for rapid mapping of protein sequence-activity relationships (ProSAR). In some embodiments, systematic ProSAR mapping can elucidate individual amino acid mutations for improved function and/or activity and/or stability etc.
Methods can be iterated to combinatorially improve the function, activity, or stability. Cassettes can be generated by oligonucleotide synthesis. Given that existing capabilities of multiplex oligonucleotide synthesis can reach over 120,000 oligonucleotides per array, combined with recombineering, the methods disclosed herein can be scaled to construct mutant libraries for dozens to hundreds of proteins in a single experiment. In some examples, at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 25, 50, 75, 100, 150, 200, 250, 300, 400, 500, 600, 700, 800, 900, 1000, or more proteins can be partially or completely covered by mutant libraries generated by the methods disclosed herein.
Disclosed herein are strategies to construct barcoded substitution libraries for several different proteins at the same time. Using existing multiplex DNA synthesis technology, as disclosed, a partial or complete substitution library for one or more protein constructs can be barcoded, or non-barcoded if desired, for one or for several hundred proteins at the same time. In some examples, such libraries comprise trackable plasmids as disclosed herein.
Some embodiments herein apply to analysis and structure/function/stability library construction of any protein with a corresponding screen or selection for activity. Cassette library size can depend on the number (N) of amino acids in a protein of interest, with a full saturation library, including all 20 amino acids at each position and optionally non-naturally occurring amino acids, scaling as 19 (or more)×N and an alanine-mapping library scaling as 1×N. Thus, in some examples, screening of even very large proteins of more than 1,000 amino acids can be tractable given current multiplex oligo synthesis capabilities of at least 120,000 oligos per array.
In addition or as an alternative to activity screens, more general properties with developed high-throughput screens and selections can be efficiently tested using methods and cassettes disclosed herein. For example, universal protein folding and solubility reporters can be engineered for expression in the cytoplasm, periplasm, and the inner membrane. In some examples, a protein library can be screened under different conditions such as different temperatures, different substrates or co-factors, in order to identify residue changes required for expression of various traits. In other embodiments, because residues can be analyzed one at a time, mutations at residues important for a particular trait, such as thermostability, resistance to environmental pressures, or increases or decreases in functionality or production, can be combined via multiplex recombineering with mutations important for various other traits, such as catalytic activity, to create combinatorial libraries for multi-trait optimization.
Methods disclosed herein can provide for creating and/or evaluating comprehensive, in vivo, mutational libraries of one or more target protein(s). These approaches can be extended via a recorder cassettes or barcoding technology to generate trackable mutational libraries for any number of residues or every residue in a protein. This approach can be based on protein sequence-activity relationship mapping method extended to work in vivo, capable of working on one or a few to hundreds of proteins simultaneously depending on the technology selected. For example, these methods permit one to map in a single experiment any number of, the majority of, or all possible residue changes over a collection of desired proteins onto a trait of interest, as part of individual proteins of interest or as part of a pathway.
In some examples, these approaches can be used at least for the following by mapping i) any number of or all residue changes for any number of or all proteins in a specific biochemical pathway, such as lycopene production, or that catalyze similar reactions, such as dehydrogenases or other enzymes of a pathway of use to produce a desired effect or produce a product, or ii) any number of or all residues in the regulatory sites of any number of or all proteins with a specific regulatory mechanism, such as heat shock response, or iii) any number of or all residues of a biological agent used to treat a health condition, such as insulin, a growth factor (HCG), an anti-cancer biologic, or a replacement protein for a deficient population.
Scores related to various input parameters can be assigned in order to generate one or more composite score(s) for designing genomically-engineered organisms or systems. These scores can reflect quality of genetic variations in genes or genetic loci as they relate to selection of an organism or design of an organism for a predetermined production, trait or traits. Certain organisms or systems can be designed based on a need for improved organisms for biorefining, biomass, such as crops, trees, grasses, crop residues, or forest residues, biofuel production, and using biological conversion, fermentation, chemical conversion and catalysis to generate and use compounds, biopharmaceutical production and biologic production. In certain embodiments, this can be accomplished by modulating growth or production of microorganism through genetic manipulation methods disclosed herein.
Genetic manipulation by methods disclosed herein of genes encoding a protein can be used to make desired genetic changes that can result in desired phenotypes and can be accomplished through numerous techniques including but not limited to, i) introduction of new genetic material, ii) genetic insertion, disruption or removal of existing genetic material, as well as, iii) mutation of genetic material, such as a point mutation, or any combinations of i, ii, and iii, that results in desired genetic changes with desired phenotypic changes. Mutations can be directed or random, in addition to those including, but not limited to, error prone or directed mutagenesis through PCR, mutator strains, and random mutagenesis. Mutations can be incorporated using trackable plasmids and methods as disclosed herein.
Disclosed methods can be used for inserting and accumulating higher order modifications into a microorganism's genome or a target protein; for example, multiple different site-specified mutations in the same genome, at high efficiency to generate libraries of genomes with over 1, 5, 10, 15, 20, 25, 30, 40, 50, 60, 70, 80, 90, 100, 150, 200, 250, 300, or more targeted modifications are described. In some examples, these mutations are within regulatory modules, regulatory elements, protein-coding regions, or non-coding regions. Protein coding modifications can include, but are not limited to, amino acid changes, codon optimization, and translation tuning.
In some instances, methods are provided for the co-delivery of reagents to a single biological cell. The methods generally involve the attachment or linkage of two or more cassettes, followed by delivery of the linked cassettes to a single cell. Generally, the methods provided herein involve the delivery of two or more cassettes to a single cell. In many cases, it is desirable that each individual cell receives the two or more cassettes. Traditional methods of reagent delivery may often be inefficient and/or inconsistent, leading to situations in which some cells receive only one of the cassettes. The methods provided herein may improve the efficiency and/or consistency of reagent delivery, such that a majority of cells in a cell population each receive the two or more cassettes. For example, more than 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% of the cells in a cell population may receive the two or more cassettes.
The two or more cassettes may be linked by any known method in the art and generally the method chosen will be commensurate with the chemistry of the cassettes. Generally, the two or more cassettes are linked by a covalent bond (i.e., covalently-linked), however, other types of non-covalent chemical bonds are envisioned, such as hydrogen bonds, ionic bonds, and metallic bonds. In this way, the editing cassette and the recorder cassette may be linked and delivered into a single cell. A known edit is then associated with a known recorder or barcode sequence for that cell.
In one example, the two or more cassettes are nucleic acids, such as two or more nucleic acids. The nucleic acids may be RNA, DNA, or a combination of both, and may contain any number of chemically-modified nucleotides or nucleotide analogues. In some cases, two or more RNA cassettes are linked for delivery to a single cell. In other cases, two or more DNA cassettes are linked for delivery to a single cell. In yet other cases, a DNA cassettes and an RNA cassettes are linked for delivery to a single cell. The nucleic acids may be derived from genomic RNA, complementary DNA (cDNA), or chemically or enzymatically synthesized DNA.
A cassettes may be of 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, about 110, about 120, about 130, about 140, about 150, about 160, about 170, about 180, about 190, about 200, about 210, about 220, about 230, about 240, about 250, about 275, about 300, about 325, about 350, about 375, about 400, about 425, about 450, about 475, about 500, about 525, about 550, about 575, about 600, about 625, about 650, about 675, about 700, about 725, about 750, about 775, about 800, about 825, about 850, about 875, about 900, about 925, about 950, about 975, about 1000, about 1100, about 1200, about 1300, about 1400, about 1500, about 1750, about 2000, about 2500, about 3000, about 4000, about 5000, about 6000, about 7000, about 8000, about 9000, about 10,000 or greater nucleotide residues in length, up to a full length protein encoding or regulatory genetic element.
Two or more cassettes may be linked on a linear nucleic acid molecule or may be linked on a plasmid or circular nucleic acid molecule. The two or more cassettes may be linked directly to one another or may be separated by one or more nucleotide spacers or linkers.
Two or more cassettes may be covalently linked on a linear cassettes or may be covalently linked on a plasmid or circular nucleic acid molecule. The two or more cassettes may be covalently linked directly to one another or may be separated by one or more nucleotide spacers or linkers.
Any number and variety of cassettes may be linked for co-delivery. For example, the two or more cassettes may include nucleic acids, lipids, proteins, peptides, small molecules, or any combination thereof. The two or more cassettes may be essentially any cassettes that are amenable to linkage.
In preferred examples, the two or more cassettes are covalently linked (e.g., by a chemical bond). Covalent linkage may help to ensure that the two or more cassettes are co-delivered to a single cell. Generally, the two or more cassettes are covalently linked prior to delivery to a cell. Any method of covalently linking two or more molecules may be utilized, and it should be understood that the methods used will be at least partly determined by the types of cassettes to be linked.
In some instances, methods are provided for the co-delivery of reagents to a single biological cell. The methods generally involve the covalent attachment or linkage of two or more cassettes, followed by delivery of the covalently-linked cassettes into a single cell. The methods provided may help to ensure that an individual cell receives the two or more cassettes. Any known method of reagent delivery may be utilized to deliver the linked cassettes to a cell and will at least partly depend on the chemistry of the cassettes to be delivered. Non-limiting examples of reagent delivery methods may include: transformation, lipofection, electroporation, transfection, nanoparticles, and the like.
In various embodiments, cassettes, or isolated, donor, or editing nucleic acids may be introduced to a cell or microorganism to alter or modulate an aspect of the cell or microorganism, for example survival or growth of the microorganism as disclosed herein. The isolated nucleic acid may be derived from genomic RNA, complementary DNA (cDNA), chemically or enzymatically synthesized DNA. Additionally or alternatively, isolated nucleic acids may be of use for capture probes, primers, labeled detection oligonucleotides, or fragments for DNA assembly.
A “nucleic acid” can include single-stranded and/or double-stranded molecules, as well as DNA, RNA, chemically modified nucleic acids and nucleic acid analogs. It is contemplated that a nucleic acid may be of 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, about 110, about 120, about 130, about 140, about 150, about 160, about 170, about 180, about 190, about 200, about 210, about 220, about 230, about 240, about 250, about 275, about 300, about 325, about 350, about 375, about 400, about 425, about 450, about 475, about 500, about 525, about 550, about 575, about 600, about 625, about 650, about 675, about 700, about 725, about 750, about 775, about 800, about 825, about 850, about 875, about 900, about 925, about 950, about 975, about 1000, about 1100, about 1200, about 1300, about 1400, about 1500, about 1750, about 2000, about 2500, about 3000, about 4000, about 5000, about 6000, about 7000, about 8000, about 9000, about 10,000 or greater nucleotide residues in length, up to a full length protein encoding or regulatory genetic element.
Isolated nucleic acids may be made by any method known in the art, for example using standard recombinant methods, assembly methods, synthetic techniques, or combinations thereof. In some embodiments, the nucleic acids may be cloned, amplified, assembled, or otherwise constructed.
The nucleic acids may conveniently comprise sequences in addition to a portion of a lysine riboswitch. For example, a multi-cloning site comprising one or more endonuclease restriction sites may be added. A nucleic acid may be attached to a vector, adapter, or linker for cloning of a nucleic acid. Additional sequences may be added to such cloning and sequences to optimize their function, to aid in isolation of the nucleic acid, or to improve the introduction of the nucleic acid into a cell. Use of cloning vectors, expression vectors, adapters, and linkers is well known in the art.
Isolated nucleic acids may be obtained from cellular, bacterial, or other sources using any number of cloning methodologies known in the art. In some embodiments, oligonucleotide probes which selectively hybridize, under stringent conditions, to other oligonucleotides or to the nucleic acids of an organism or cell. Methods for construction of nucleic acid libraries are known and any such known methods may be used.
Cellular genomic DNA, RNA, or cDNA may be screened for the presence of an identified genetic element of interest using a probe based upon one or more sequences. Various degrees of stringency of hybridization may be employed in the assay.
High stringency conditions for nucleic acid hybridization are well known in the art. For example, conditions may comprise low salt and/or high temperature conditions, such as provided by about 0.02 M to about 0.15 M NaCl at temperatures of about 50° C. to about 70° C. It is understood that the temperature and ionic strength of a desired stringency are determined in part by the length of the particular nucleic acid(s), the length and nucleotide content of the target sequence(s), the charge composition of the nucleic acid(s), and by the presence or concentration of formamide, tetramethylammonium chloride or other solvent(s) in a hybridization mixture. Nucleic acids may be completely complementary to a target sequence or may exhibit one or more mismatches.
Nucleic acids of interest may also be amplified using a variety of known amplification techniques. For instance, polymerase chain reaction (PCR) technology may be used to amplify target sequences directly from DNA, RNA, or cDNA. PCR and other in vitro amplification methods may also be useful, for example, to clone nucleic acid sequences, to make nucleic acids to use as probes for detecting the presence of a target nucleic acid in samples, for nucleic acid sequencing, or for other purposes.
Isolated nucleic acids may be prepared by direct chemical synthesis by methods such as the phosphotriester method, or using an automated synthesizer. Chemical synthesis generally produces a single stranded oligonucleotide. This may be converted into double stranded DNA by hybridization with a complementary sequence or by polymerization with a DNA polymerase using the single strand as a template.
Any method known in the art for identifying, isolating, purifying, using and assaying activities of target proteins contemplated herein are contemplated. Target proteins contemplated herein include protein agents used to treat a human condition or to regulate processes (e.g. part of a pathway such as an enzyme) involved in disease of a human or non-human mammal. Any method known for selection and production of antibodies or antibody fragments is also contemplated. Additionally or alternatively, target proteins can be proteins or enzymes involved in a pathway or process in a virus, cell, or organism.
Targetable Nucleic Acid Cleavage Systems Some methods disclosed herein comprise targeting cleavage of specific nucleic acid sequences using a site-specific, targetable, and/or engineered nuclease or nuclease system. Such nucleases can create double-stranded break (DSBs) at desired locations in a genome or nucleic acid molecule. In other examples, a nuclease can create a single strand break. In some cases, two nucleases are used, each of which generates a single strand break.
The one or more double or single strand break can be repaired by natural processes of homologous recombination (HR) and non-homologous end-joining (NHEJ) using the cell's endogenous machinery. Additionally or alternatively, endogenous or heterologous recombination machinery can be used to repair the induced break or breaks.
Engineered nucleases such as zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), engineered homing endonucleases, and RNA or DNA guided endonucleases, such as CRISPR/Cas such as Cas9 or CPF1, and/or Argonaute systems, are particularly appropriate to carry out some of the methods of the present invention. Additionally or alternatively, RNA targeting systems can use used, such as CRISPR/Cas systems including c2c2 nucleases.
Methods disclosed herein can comprise cleaving a target nucleic acid using a CRISPR systems, such as a Type I, Type II, Type III, Type IV, Type V, or Type VI CRISPR system. CRISPR/Cas systems can be multi-protein systems or single effector protein systems. Multi-protein, or Class 1, CRISPR systems include Type I, Type III, and Type IV systems. Alternatively, Class 2 systems include a single effector molecule and include Type II, Type VI, and Type VI.
CRISPR systems used in methods disclosed herein can comprise a single or multiple effector proteins. An effector protein can comprise one or multiple nuclease domains. An effector protein can target DNA or RNA, and the DNA or RNA may be single stranded or double stranded. Effector proteins can generate double strand or single strand breaks. Effector proteins can comprise mutations in a nuclease domain thereby generating a nickase protein. Effector proteins can comprise mutations in one or more nuclease domains, thereby generating a catalytically dead nuclease that is able to bind but not cleave a target sequence. CRISPR systems can comprise a single or multiple guiding RNAs. The gRNA can comprise a crRNA. The gRNA can comprise a chimeric RNA with crRNA and tracrRNA sequences. The gRNA can comprise a separate crRNA and tracrRNA. Target nucleic acid sequences can comprise a protospacer adjacent motif (PAM) or a protospacer flanking site (PFS). The PAM or PFS may be 3′ or 5′ of the target or protospacer site. Cleavage of a target sequence may generate blunt ends, 3′ overhangs, or 5′ overhangs.
A gRNA can comprise a spacer sequence. Spacer sequences can be complementary to target sequences or protospacer sequences. Spacer sequences can be 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, or 36 nucleotides in length. In some examples, the spacer sequence can be less than 10 or more than 36 nucleotides in length.
A gRNA can comprise a repeat sequence. In some cases, the repeat sequence is part of a double stranded portion of the gRNA. A repeat sequence can be 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 nucleotides in length. In some examples, the spacer sequence can be less than 10 or more than 50 nucleotides in length.
A gRNA can comprise one or more synthetic nucleotides, non-naturally occurring nucleotides, nucleotides with a modification, deoxyribonucleotide, or any combination thereof. Additionally or alternatively, a gRNA may comprise a hairpin, linker region, single stranded region, double stranded region, or any combination thereof. Additionally or alternatively, a gRNA may comprise a signaling or reporter molecule.
A CRISPR nuclease can be endogenously or recombinantly expressed within a cell. A CRISPR nuclease can be encoded on a chromosome, extrachromosomally, or on a plasmid, synthetic chromosome, or artificial chromosome. A CRISPR nuclease can be provided or delivered to the cell as a polypeptide or mRNA encoding the polypeptide. In such examples, polypeptide or mRNA can be delivered through standard mechanisms known in the art, such as through the use of cell permeable peptides, nanoparticles, or viral particles.
gRNAs can be encoded by genetic or episomal DNA within a cell. In some examples, gRNAs can be provided or delivered to a cell expressing a CRISPR nuclease. gRNAs can be provided or delivered concomitantly with a CRISPR nuclease or sequentially. Guide RNAs can be chemically synthesized, in vitro transcribed, or otherwise generated using standard RNA generation techniques known in the art.
A CRISPR system can be a Type II CRISPR system, for example a Cas9 system. The Type II nuclease can comprise a single effector protein, which, in some cases, comprises a RuvC and HNH nuclease domains. In some cases a functional Type II nuclease can comprise two or more polypeptides, each of which comprises a nuclease domain or fragment thereof. The target nucleic acid sequences can comprise a 3′ protospacer adjacent motif (PAM). In some examples, the PAM may be 5′ of the target nucleic acid. Guide RNAs (gRNA) can comprise a single chimeric gRNA, which contains both crRNA and tracrRNA sequences. Alternatively, the gRNA can comprise a set of two RNAs, for example a crRNA and a tracrRNA. The Type II nuclease can generate a double strand break, which is some cases creates two blunt ends. In some cases, the Type II CRISPR nuclease is engineered to be a nickase such that the nuclease only generates a single strand break. In such cases, two distinct nucleic acid sequences can be targeted by gRNAs such that two single strand breaks are generated by the nickase. In some examples, the two single strand breaks effectively create a double strand break. In some cases where a Type II nickase is used to generate two single strand breaks, the resulting nucleic acid free ends can either be blunt, have a 3′ overhang, or a 5′ overhang. In some examples, a Type II nuclease may be catalytically dead such that it binds to a target sequence, but does not cleave. For example, a Type II nuclease could have mutations in both the RuvC and HNH domains, thereby rendering the both nuclease domains non-functional. A Type II CRISPR system can be one of three sub-types, namely Type II-A, Type II-B, or Type II-C.
A CRISPR system can be a Type V CRISPR system, for example a Cpf1, C2c1, or C2c3 system. The Type V nuclease can comprise a single effector protein, which in some cases comprises a single RuvC nuclease domain. In other cases, a function Type V nuclease comprises a RuvC domain split between two or more polypeptides. In such cases, the target nucleic acid sequences can comprise a 5′ PAM or 3′ PAM. Guide RNAs (gRNA) can comprise a single gRNA or single crRNA, such as can be the case with Cpf1. In some cases, a tracrRNA is not needed. In other examples, such as when C2c1 is used, a gRNA can comprise a single chimeric gRNA, which contains both crRNA and tracrRNA sequences or the gRNA can comprise a set of two RNAs, for example a crRNA and a tracrRNA. The Type V CRISPR nuclease can generate a double strand break, which in some cases generates a 5′ overhang. In some cases, the Type V CRISPR nuclease is engineered to be a nickase such that the nuclease only generates a single strand break. In such cases, two distinct nucleic acid sequences can be targeted by gRNAs such that two single strand breaks are generated by the nickase. In some examples, the two single strand breaks effectively create a double strand break. In some cases where a Type V nickase is used to generate two single strand breaks, the resulting nucleic acid free ends can either be blunt, have a 3′ overhang, or a 5′ overhang. In some examples, a Type V nuclease may be catalytically dead such that it binds to a target sequence, but does not cleave. For example, a Type V nuclease could have mutations a RuvC domain, thereby rendering the nuclease domain non-functional.
A CRISPR system can be a Type VI CRISPR system, for example a C2c2 system. A Type VI nuclease can comprise a HEPN domain. In some examples, the Type VI nuclease comprises two or more polypeptides, each of which comprises a HEPN nuclease domain or fragment thereof. In such cases, the target nucleic acid sequences can by RNA, such as single stranded RNA. When using Type VI CRISPR system, a target nucleic acid can comprise a protospacer flanking site (PFS). The PFS may be 3′ or 5′ or the target or protospacer sequence. Guide RNAs (gRNA) can comprise a single gRNA or single crRNA. In some cases, a tracrRNA is not needed. In other examples, a gRNA can comprise a single chimeric gRNA, which contains both crRNA and tracrRNA sequences or the gRNA can comprise a set of two RNAs, for example a crRNA and a tracrRNA. In some examples, a Type VI nuclease may be catalytically dead such that it binds to a target sequence, but does not cleave. For example, a Type VI nuclease could have mutations in a HEPN domain, thereby rendering the nuclease domains non-functional.
Non-limiting examples of suitable nucleases, including nucleic acid-guided nucleases, for use in the present disclosure include C2c1, C2c2, C2c3, Cas1, Cas1B, Cas2, Cas3, Cas4, Cas5, Cas6, Cas7, Cas8, Cas9 (also known as Csn1 and Csx12), Cas10, Cpf1, Csy1, Csy2, Csy3, Cse1, Cse2, Csc1, Csc2, Csa5, Csn2, Csm2, Csm3, Csm4, Csm5, Csm6, Cmr1, Cmr3, Cmr4, Cmr5, Cmr6, Csb1, Csb2, Csb3, Csx17, Csx14, Csx100, Csx16, CsaX, Csx3, Csx1, Csx15, Csf1, Csf2, Csf3, Csf4, homologues thereof, orthologues thereof, or modified versions thereof. Suitable nucleic acid-guided nucleases can be from an organism from a genus which includes but is not limited to Thiomicrospira, Succinivibrio, Candidatus, Porphyromonas, Acidomonococcus, Prevotella, Smithella, Moraxella, Synergistes, Francisella, Leptospira, Catenibacterium, Kandleria, Clostridium, Dorea, Coprococcus, Enterococcus, Fructobacillus, Weissella, Pediococcus, Corynebacter, Sutterella, Legionella, Treponema, Roseburia, Filifactor, Eubacterium, Streptococcus, Lactobacillus, Mycoplasma, Bacteroides, Flaviivola, Flavobacterium, Sphaerochaeta, Azospirillum, Gluconacetobacter, Neisseria, Roseburia, Parvibaculum, Staphylococcus, Nitratifractor, Mycoplasma, Alicyclobacillus, Brevibacilus, Bacillus, Bacteroidetes, Brevibacilus, Carnobacterium, Clostridiaridium, Clostridium, Desulfonatronum, Desulfovibrio, Helcococcus, Leptotrichia, Listeria, Methanomethyophilus, Methylobacterium, Opitutaceae, Paludibacter, Rhodobacter, Sphaerochaeta, Tuberibacillus, and Campylobacter. Species of organism of such a genus can be as otherwise herein discussed. Suitable nucleic acid-guided nucleases can be from an organism from a genus or unclassified genus within a kingdom, which includes but is not limited to Firmicute, Actinobacteria, Bacteroidetes, Proteobacteria, Spirochates, and Tenericutes. Suitable nucleic acid-guided nucleases can be from an organism from a genus or unclassified genus within a phylum which includes but is not limited to Erysipelotrichia, Clostridia, Bacilli, Actinobacteria, Bacteroidetes, Flavobacteria, Alphaproteobacteria, Betaproteobacteria, Gammaproteob acteria, Deltaproteob acteria, Epsilonproteobacteria, Spirochaetes, and Mollicutes. Suitable nucleic acid-guided nucleases can be from an organism from a genus or unclassified genus within an order which includes but is not limited to Clostridiales, Lactobacillales, Actinomycetales, Bacteroidales, Flavobacteriales, Rhizobiales, Rhodospirillales, Burkholderiales, Neisseriales, Legionellales, Nautiliales, Campylobacterales, Spirochaetales, Mycoplasmatales, and Thiotrichales. Suitable nucleic acid-guided nucleases can be from an organism from a genus or unclassified genus within a family which includes but is not limited to Lachnospiraceae, Enterococcaceae, Leuconostocaceae, Lactobacillaceae, Streptococcaceae, Peptostreptococcaceae, Staphylococcaceae, Eubacteriaceae, Corynebacterineae, Bacteroidaceae, Flavobacterium, Cryomoorphaceae, Rhodobiaceae, Rhodospirillaceae, Acetobacteraceae, Sutterellaceae, Neisseriaceae, Legionellaceae, Nautiliaceae, Campylobacteraceae, Spirochaetaceae, Mycoplasmataceae, Pisciririckettsiaceae, and Francisellaceae.
Other nucleic acid-guided nucleases suitable for use in the methods, systems, and compositions of the present disclosure include those derived from an organism such as, but not limited to, Thiomicrospira sp. XS5, Eubacterium rectale, Succinivibrio dextrinosolvens, Candidatus Methanoplasma termitum, Candidatus Methanomethylophilus alvus, Porphyromonas crevioricanis, Flavobacterium branchiophilum, Acidomonococcus sp., Lachnospiraceae bacterium COE1, Prevotella brevis ATCC 19188, Smithella sp. SCADC, Moraxella bovoculi, Synergistes jonesii, Bacteroidetes oral taxon 274, Francisella tularensis, Leptospira inadai serovar Lyme str. 10, Acidomonococcus sp. crystal structure (5B43) S. mutans, S. agalactiae, S. equisimilis, S. sanguinis, S. pneumonia; C. jejuni, C. coli; N. salsuginis, N. tergarcus; S. auricularis, S. carnosus; N. meningitides, N. gonorrhoeae; L. monocytogenes, L. ivanovii; C. botulinum, C. difficile, C. tetani, C. sordellii; Francisella tularensis 1, Prevotella albensis, Lachnospiraceae bacterium MC2017 1, Butyrivibrio proteoclasticus, Peregrinibacteria bacterium GW2011_GWA2_33_10, Parcubacteria bacterium GW2011_GWC2_44_17, Smithella sp. SCADC, Acidaminococcus sp. BV3L6, Lachnospiraceae bacterium MA2020, Candidatus Methanoplasma termitum, Eubacterium eligens, Moraxella bovoculi 237, Leptospira inadai, Lachnospiraceae bacterium ND2006, Porphyromonas crevioricanis 3, Prevotella disiens, Porphyromonas macacae, Catenibacterium sp. CAG:290, Kandleria vitulina, Clostridiales bacterium KA00274, Lachnospiraceae bacterium 3-2, Dorea longicatena, Coprococcus catus GD/7, Enterococcus columbae DSM 7374, Fructobacillus sp. EFB-N1, Weissella halotolerans, Pediococcus acidilactici, Lactobacillus curvatus, Streptococcus pyogenes, Lactobacillus versmoldensis, and Filifactor alocis ATCC 35896.
Suitable nucleases for use in any of the methods disclosed herein include, but are not limited to, nucleases having the sequences listed in Table 1, or homologues having at least 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or 99% sequence identity to any of the nucleases listed in Table 1.
TABLE 1
MAD Amino acid
nuclease sequence
MAD1 SEQ ID NO: 1
MAD2 SEQ ID NO: 2
MAD3 SEQ ID NO: 3
MAD4 SEQ ID NO: 4
MAD5 SEQ ID NO: 5
MAD6 SEQ ID NO: 6
MAD7 SEQ ID NO: 7
MAD8 SEQ ID NO: 8
MAD9 SEQ ID NO: 9
MAD10 SEQ ID NO: 10
MAD11 SEQ ID NO: 11
MAD12 SEQ ID NO: 12
MAD13 SEQ ID NO: 13
MAD14 SEQ ID NO: 14
MAD15 SEQ ID NO: 15
MAD16 SEQ ID NO: 16
MAD17 SEQ ID NO: 17
MAD18 SEQ ID NO: 18
MAD19 SEQ ID NO: 19
MAD20 SEQ ID NO: 20
In some methods disclosed herein, Argonaute (Ago) systems can be used to cleave target nucleic acid sequences. Ago protein can be derived from a prokaryote, eukaryote, or archaea. The target nucleic acid may be RNA or DNA. A DNA target may be single stranded or double stranded. In some examples, the target nucleic acid does not require a specific target flanking sequence, such as a sequence equivalent to a protospacer adjacent motif or protospacer flanking sequence. The Ago protein may create a double strand break or single strand break. In some examples, when a Ago protein forms a single strand break, two Ago proteins may be used in combination to generate a double strand break. In some examples, an Ago protein comprises one, two, or more nuclease domains. In some examples, an Ago protein comprises one, two, or more catalytic domains. One or more nuclease or catalytic domains may be mutated in the Ago protein, thereby generating a nickase protein capable of generating single strand breaks. In other examples, mutations in one or more nuclease or catalytic domains of an Ago protein generates a catalytically dead Ago protein that can bind but not cleave a target nucleic acid.
Ago proteins can be targeted to target nucleic acid sequences by a guiding nucleic acid. In many examples, the guiding nucleic acid is a guide DNA (gDNA). The gDNA can have a 5′ phosphorylated end. The gDNA can be single stranded or double stranded. Single stranded gDNA can be 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 nucleotides in length. In some examples, the gDNA can be less than 10 nucleotides in length. In some examples, the gDNA can be more than 50 nucleotides in length.
Argonaute-mediated cleavage can generate blunt end, 5′ overhangs, or 3′ overhangs. In some examples, one or more nucleotides are removed from the target site during or following cleavage.
Argonaute protein can be endogenously or recombinantly expressed within a cell. Argonaute can be encoded on a chromosome, extrachromosomally, or on a plasmid, synthetic chromosome, or artificial chromosome. Additionally or alternatively, an Argonaute protein can be provided or delivered to the cell as a polypeptide or mRNA encoding the polypeptide. In such examples, polypeptide or mRNA can be delivered through standard mechanisms known in the art, such as through the use of cell permeable peptides, nanoparticles, or viral particles.
Guide DNAs can be provided by genetic or episomal DNA within a cell. In some examples, gDNA are reverse transcribed from RNA or mRNA within a cell. In some examples, gDNAs can be provided or delivered to a cell expressing an Ago protein. Guide DNAs can be provided or delivered concomitantly with an Ago protein or sequentially. Guide DNAs can be chemically synthesized, assembled, or otherwise generated using standard DNA generation techniques known in the art. Guide DNAs can be cleaved, released, or otherwise derived from genomic DNA, episomal DNA molecules, isolated nucleic acid molecules, or any other source of nucleic acid molecules.
In some instances, compositions are provided comprising a nuclease such as an nucleic acid-guided nuclease (e.g., Cas9, Cpf1, MAD2, or MAD7) or a DNA-guided nuclease (e.g., Ago), linked to a chromatin-remodeling enzyme. Without wishing to be bound by theory, a nuclease fusion protein as described herein may provide improved accessibility to regions of highly-structured DNA. Non-limiting examples of chromatin-remodeling enzymes that can be linked to a nucleic-acid guided nuclease may include: histone acetyl transferases (HATs), histone deacetylases (HDACs), histone methyltransferases (HMTs), chromatin remodeling complexes, and transcription activator-like (Tal) effector proteins. Histone deacetylases may include HDAC1, HDAC2, HDAC3, HDAC4, HDACS, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, sirtuin 1, sirtuin 2, sirtuin 3, sirtuin 4, sirtuin 5, sirtuin 6, and sirtuin 7. Histone acetyl transferases may include GCNS, PCAF, Hat1, Elp3, Hpa2, Hpa3, ATF-2, Nut1, Esa1, Sas2, Sas3, Tip60, MOF, MOZ, MORF, HBO1, p300, CBP, SRC-1, ACTR, TIF-2, SRC-3, TAFII250, TFIIIC, Rtt109, and CLOCK. Histone methyltransferases may include ASH1L, DOT1L, EHMT1, EHMT2, EZH1, EZH2, MLL, MLL2, MLL3, MLL4, MLL5, NSD1, PRDM2, SET, SETBP1, SETD1A, SETD1B, SETD2, SETD3, SETD4, SETD5, SETD6, SETD7, SETD8, SETD9, SETDB1, SETDB2, SETMAR, SMYD1, SMYD2, SMYD3, SMYD4, SMYD5, SUV39H1, SUV39H2, SUV420H1, and SUV420H2. Chromatin-remodeling complexes may include SWI/SNF, ISWI, NuRD/Mi-2/CHD, INO80 and SWR1.
In some instances, the nuclease is a wild-type nuclease. In other instances, the nuclease is a chimeric engineered nuclease. Chimeric engineered nucleases as disclosed herein can comprise one or more fragments or domains, and the fragments or domains can be of a nuclease, such as nucleic acid-guided nuclease, orthologs of organisms of genuses, species, or other phylogenetic groups disclosed herein; advantageously the fragments are from nuclease orthologs of different species. A chimeric engineered nuclease can be comprised of fragments or domains from at least two different nucleases. A chimeric engineered nuclease can be comprised of fragments or domains from at least two different species. A chimeric engineered nuclease can be comprised of fragments or domains from at least 2, 3, 4, 5, 6, 7, 8, 9, 10, or more different nucleases or different species. In some cases, more than one fragment or domain from one nuclease or species, wherein the more than one fragment or domain are separated by fragments or domains from a second nuclease or species. In some examples, a chimeric engineered nuclease comprises 2 fragments, each from a different protein or nuclease. In some examples, a chimeric engineered nuclease comprises 3 fragments, each from a different protein or nuclease. In some examples, a chimeric engineered nuclease comprises 4 fragments, each from a different protein or nuclease. In some examples, a chimeric engineered nuclease comprises 5 fragments, each from a different protein or nuclease.
Nuclease fusion proteins can be recombinantly expressed within a cell. A nuclease fusion protein can be encoded on a chromosome, extrachromosomally, or on a plasmid, synthetic chromosome, or artificial chromosome. A nuclease and a chromatin-remodeling enzyme may be engineered separately, and then covalently linked, prior to delivery to a cell. A nuclease fusion protein can be provided or delivered to the cell as a polypeptide or mRNA encoding the polypeptide. In such examples, polypeptide or mRNA can be delivered through standard mechanisms known in the art, such as through the use of cell permeable peptides, nanoparticles, or viral particles.
Cell-Cycle-Dependent Expression of Targeted Nucleases. In some instances, compositions comprising a cell-cycle-dependent nuclease are provided. A cell-cycle dependent nuclease generally includes a targeted nuclease as described herein linked to an enzyme that leads to degradation of the targeted nuclease during G1 phase of the cell cycle, and expression of the targeted nuclease during G2/M phase of the cell cycle. Such cell-cycle dependent expression may, for example, bias the expression of the nuclease in cells where homology-directed repair (HDR) is most active (e.g., during G2/M phase). In some cases, the nuclease is covalently linked to cell-cycle regulated protein such as one that is actively degraded during G1 phase of the cell cycle and is actively expressed during G2/M phase of the cell cycle. In a non-limiting example, the cell-cycle regulated protein is Geminin. Other non-limiting examples of cell-cycle regulated proteins may include: Cyclin A, Cyclin B, Hsll, Cdc6, Finl, p21 and Skp2.
In some instances, the nuclease is a wild-type nuclease.
In other instances, the nuclease is a engineered nuclease. Engineered nucleases can be non-naturally occurring.
Non-naturally occurring targetable nucleases and non-naturally occurring targetable nuclease systems can address many of these challenges and limitations.
Disclosed herein are non-naturally targetable nuclease systems. Such targetable nuclease systems are engineered to address one or more of the challenges described above and can be referred to as engineered nuclease systems. Engineered nuclease systems can comprise one or more of an engineered nuclease, such as an engineered nucleic acid-guided nuclease, an engineered guide nucleic acid, an engineered polynucleotides encoding said nuclease, or an engineered polynucleotides encoding said guide nucleic acid. Engineered nucleases, engineered guide nucleic acids, and engineered polynucleotides encoding the engineered nuclease or engineered guide nucleic acid are not naturally occurring and are not found in nature. It follows that engineered nuclease systems including one or more of these elements are non-naturally occurring.
Non-limiting examples of types of engineering that can be done to obtain a non-naturally occurring nuclease system are as follows. Engineering can include codon optimization to facilitate expression or improve expression in a host cell, such as a heterologous host cell. Engineering can reduce the size or molecular weight of the nuclease in order to facilitate expression or delivery. Engineering can alter PAM selection in order to change PAM specificity or to broaden the range of recognized PAMs. Engineering can alter, increase, or decrease stability, processivity, specificity, or efficiency of a targetable nuclease system. Engineering can alter, increase, or decrease protein stability. Engineering can alter, increase, or decrease processivity of nucleic acid scanning. Engineering can alter, increase, or decrease target sequence specificity. Engineering can alter, increase, or decrease nuclease activity. Engineering can alter, increase, or decrease editing efficiency. Engineering can alter, increase, or decrease transformation efficiency. Engineering can alter, increase, or decrease nuclease or guide nucleic acid expression.
Examples of non-naturally occurring nucleic acid sequences which are disclosed herein include sequences codon optimized for expression in bacteria, such as E. coli (e.g., SEQ ID NO: 41-60), sequences codon optimized for expression in single cell eukaryotes, such as yeast (e.g., SEQ ID NO: 127-146), sequences codon optimized for expression in multi cell eukaryotes, such as human cells (e.g., SEQ ID NO: 147-166), polynucleotides used for cloning or expression of any sequences disclosed herein (e.g., SEQ ID NO: 61-80), plasmids comprising nucleic acid sequences (e.g., SEQ ID NO: 21-40) operably linked to a heterologous promoter or nuclear localization signal or other heterologous element, proteins generated from engineered or codon optimized nucleic acid sequences (e.g., SEQ ID NO: 1-20), or engineered guide nucleic acids comprising any one of SEQ ID NO: 84-107. Such non-naturally occurring nucleic acid sequences can be amplified, cloned, assembled, synthesized, generated from synthesized oligonucleotides or dNTPs, or otherwise obtained using methods known by those skilled in the art.
Additional examples of non-naturally occurring nucleic acid sequences which are disclosed herein include sequences codon optimized for expression in bacteria, such as E. coli (e.g., SEQ ID NO: 168), sequences codon optimized for expression in single cell eukaryotes, such as yeast (e.g., SEQ ID NO: 169), sequences codon optimized for expression in multi cell eukaryotes, such as human cells (e.g., SEQ ID NO: 170), polynucleotides used for cloning or expression of any sequences disclosed herein (e.g., SEQ ID NO: 171), plasmids comprising nucleic acid sequences (e.g., SEQ ID NO: 167) operably linked to a heterologous promoter or nuclear localization signal or other heterologous element, proteins generated from engineered or codon optimized nucleic acid sequences (e.g., SEQ ID NO: 108-110), or engineered guide nucleic acids compatible with any targetable nuclease disclosed herein. Such non-naturally occurring nucleic acid sequences can be amplified, cloned, assembled, synthesized, generated from synthesized oligonucleotides or dNTPs, or otherwise obtained using methods known by those skilled in the art.
A guide nucleic acid can be DNA. A guide nucleic acid can be RNA. A guide nucleic acid can comprise both DNA and RNA. A guide nucleic acid can comprise modified of non-naturally occurring nucleotides. In cases where the guide nucleic acid comprises RNA, the RNA guide nucleic acid can be encoded by a DNA sequence on a polynucleotide molecule such as a plasmid, linear construct, or editing cassette as disclosed herein.
Nucleic acid-guided nucleases can be compatible with guide nucleic acids that are not found within the nucleases endogenous host. Such orthogonal guide nucleic acids can be determined by empirical testing. Orthogonal guide nucleic acids can come from different bacterial species or be synthetic or otherwise engineered to be non-naturally occurring.
Orthogonal guide nucleic acids that are compatible with a common nucleic acid-guided nuclease can comprise one or more common features. Common features can include sequence outside a pseudoknot region. Common features can include a pseudoknot region (e.g., 172-181). Common features can include a primary sequence or secondary structure.
A guide nucleic acid can be engineered to target a desired target sequence by altering the guide sequence such that the guide sequence is complementary to the target sequence, thereby allowing hybridization between the guide sequence and the target sequence. A guide nucleic acid with an engineered guide sequence can be referred to as an engineered guide nucleic acid. Engineered guide nucleic acids are often non-naturally occurring and are not found in nature.
In other instances, the nuclease is a chimeric nuclease. Chimeric nucleases can be engineered nucleases. Chimeric nucleases as disclosed herein can comprise one or more fragments or domains, and the fragments or domains can be of a nuclease, such as nucleic acid-guided nuclease, orthologs of organisms of genuses, species, or other phylogenetic groups; advantageously the fragments are from nuclease orthologs of different species. A chimeric nuclease can be comprised of fragments or domains from at least two different nucleases. A chimeric nuclease can be comprised of fragments or domains from at least two different species. A chimeric nuclease can be comprised of fragments or domains from at least 2, 3, 4, 5, 6, 7, 8, 9, 10, or more different nucleases or different species. In some cases, more than one fragment or domain from one nuclease or species, wherein the more than one fragment or domain are separated by fragments or domains from a second nuclease or species. In some examples, a chimeric nuclease comprises 2 fragments, each from a different protein or nuclease. In some examples, a chimeric nuclease comprises 3 fragments, each from a different protein or nuclease. In some examples, a chimeric nuclease comprises 4 fragments, each from a different protein or nuclease. In some examples, a chimeric nuclease comprises 5 fragments, each from a different protein or nuclease.
EXAMPLES Example 1— CREATE-Plasmids and Libraries FIGS. 1A-C depict an example of an overview of CRISPR EnAbled Trackable genome Engineering (CREATE) design and workflow. FIG. 1A shows an example of the CREATE methodology which allows programmatic genome modifications to be focused on key amino acid residues or promoter targets across the genome. Such libraries thus enable systematic assessment of sequence/activity relationships for a wide variety of genomic targets in parallel. FIG. 1B depicts an example of CREATE cassettes designed to encode both homology arm (HA) and guide RNA (gRNA) sequences to target a specific locus in the E. coli genome. The 100 bp homology arm was designed to introduce a specific codon mutation (target codon) that can be selectively enriched by a synonymous PAM mutation to rescue the sequence from Cas9 cleavage and allow highly efficient mutagenesis. The P1 and P2 sites (black) serve as general priming sites allowing multiplexed amplification, cloning and sequencing of many libraries in parallel. The promoter (J23119, green) is a constitutive promoter that drives expression of the gRNA. Detailed example the HA design for introducing a stop codon at residue 145 in the galK locus is also depicted at the bottom of FIG. 1B. The top sequence shows the wildtype genome sequence with the PAM (CCG; the reverse complement of which is CGG, which is recognized by S. pyogenes Cas9) and target codon (TAT, encoding Y) highlighted. The HA design introduces a “silent scar” at the PAM site (CgG, the reverse complement of which is CCG, which is not recognized by S. pyogenes Cas9) and a single nucleotide TAT>TAA mutation at codon 145 (resulting in a STOP). This design strategy was implemented programmatically for coding regions across the genome. FIG. 1C depicts an overview of an example CREATE workflow. CREATE cassettes are synthesized on a microarray delivered as large oligo pools (104 to 106 individual library members). Parallel cloning and recombineering allowed processing of these pools into genomic libraries, in some cases in 23 days. Deep sequencing of the CREATE plasmids can be used to track the fitness of thousands of precision mutations genome wide following selection or screening of the mutant libraries.
Example 2— CREATE Plasmid Validation FIG. 2A-D depicts an example of the effect of Cas9 activity on transformation and editing efficiencies. The galK 120/17 CREATE cassette (120 bp HA and 17 bp PAM/codon spacing) targeting codon 145 in galK gene or a control non-targeting gRNA vector was transformed in cells carrying pSIMS along with dCas9 (e.g. left set of bars in FIG. 2A) or Cas9 (e.g. right set of bars in FIG. 2A) plasmids. The pSIMS plasmid carries lambda red recombination machinery. The cas9 gene was cloned into the pBTBX-2 backbone under the control of a pBAD promoter to allow control of the cleavage activity by addition of arabinose. Transformation efficiencies of each vector are shown with dark grey bars. The total number of recombinant cells (light grey bars) were calculated based on red/white colony screening on MacConkey agar. In cases where white colonies were undetectable by plate based screening we assumed 104 editing efficiencies. A 102 fold reduction in transformation efficiency compared to the non-targeting gRNA control was also observed for CREATE cassettes transformed into the Cas9 background.
FIG. 2B depicts an example of the characterization of CREATE cassette HA length and PAM/codon spacing on editing efficiency. All cassettes were designed to introduce a TAA stop at codon 145 in the gene using PAMs at the indicated distance (PAM/codon bottom) from the target codon and variable homology arms lengths (HA, bottom). Dark grey and light grey bars correspond to uninduced or induced expression of Cas9 under the pBAD promoter using 0.2% arabinose. In the majority of cases the editing efficiency appears to be unaffected by induction suggesting that low amounts of Cas9 due to leaky expression are sufficient for high efficiency editing.
FIG. 2C shows example data from sequencing of the genomic loci from CREATE recombineering reactions. The galK cassettes from FIG. 2B are labeled according to the HA length and PAM codon spacing. The other loci shown were cassettes isolated from multiplexed library cloning reactions. The bar plot (FIG. 2C) indicates the number of times each genotype was observed by genomic colony sequencing following recombineering with each CREATE cassette. The + and labels at the bottom indicate the presence or absence of the designed mutation at the two relevant sites in each clone. The circular inset indicates the relative position of each gene on the E. coli genome.
FIG. 2D depicts an example of library coverage from multiplexed cloning of CREATE plasmids. Deep sequencing counts each variant are shown with respect to their position on the genome. The inset shows a histogram of these plasmid counts for the entire library. The distribution follows expected Poisson distribution for low average counts.
Example 3— CREATE-Recording Used to Engineer Trackable Episomal DNA Libraries FIG. 3A depicts an example of an overview of the method used to generate a trackable episomal DNA library. Transformation of a CREATE recorder plasmid generates modifications of the target DNA at two sites. One edit occurs to the desired target gene (gray) introducing a codon or promoter mutation designed to test specific engineering objectives. The second edit targets a functionally neutral site and introduces a 15 nucleotide barcode (BC, black). By virtue of coupling these libraries on a single CREATE plasmid the target DNA is edited at both sites and each unique barcode can be used to track edits throughout the rest of the plasmid.
FIG. 2B depicts an example of the CREATE barcode design. A degenerate library is constructed from overlapping oligos and cloned in a separate site of the CREATE vector to make a library of CREATE recorder cassettes that can be coupled to the designer editing libraries.
FIG. 2C depicts an exemplary CREATE record mapping strategy. Deep sequencing of both the target DNA (left) and CREATE plasmids allows a simple sequence mapping strategy by allowing each editing cassette to be uniquely assigned by the barcode sequence. This allows the relative fitness of each barcode (and thus edit) to be tracked during selection or screening processes and can be shuttled between different organisms using standard vectors.
Example 4— CREATE-Mediated Editing of Episomal DNA Methods and compositions disclosed herein were used to mutate a key residue of the cas9 gene used for the CREATE process (e.g. FIG. 4A-4B). A cassette was designed to make an R1335K mutation in the Cas9 protein. This cassette was cloned into a CREATE plasmid and transformed into MG1655 E. coli carrying the pSIM5 and X2-Cas9 vectors. The pSIM5 vector comprises lambda red recombination machinery. The X2-Cas9 vector comprises an arabinose-inducible Cas9 expression cassette. Following three hours recovery in LB supplemented with 0.4% arabinose to induce Cas9 expression, the cells were plated on agar containing antibiotics that maintain selective pressure for replication of both the X2-Cas9 and CREATE plasmids. Colony PCR of random clones revealed the designed edits from the CREATE plasmid were efficiently transferred into the X2-Cas9 plasmid (e.g. FIG. 4B). Of the clones that were sequenced, 100% contained the silent PAM mutation in X2Cas9 and 6/14 (43%) also containing desired coding edit. This is the first demonstration that plasmid based editing using CREATE is robust despite higher copy numbers associated with the plasmid target as compared to previous genome engineering efforts.
Example 5—CREATE-Mediated Editing and Tracking of E. coli Genome-Double Cassette To test the performance of the recording strategy in a genomic context we tested the ability to edit two distal genomic loci in the E. coli genome (e.g. FIG. 5A). To do so we cloned CREATE recording cassette libraries designed to embed the 15 nucleotide barcodes into the galK locus. After cloning, we isolated a few unique barcodes and cloned a second editing cassette designed to incorporate an F153R mutation in the dihydrofolate reductase (DHFR)/folA gene that was identified by our previous CREATE studies as conferring tolerance to the antibiotic trimethoprim. Genotyping of E. coli strains following transformation of the dual CREATE recording vector according to previously described protocols yielded the data in FIG. 5A. The efficiency of barcoding (100%) was higher than the target genome edit (80-90%), ensuring that edited genomes can be tracked. Of the transformed population we observed >80% of colonies contained the barcode edit in the galK locus as determined by red white colony screening (e.g. FIG. 5B). From the barcoded colonies we found that 85% of the colonies also encoded the DHFR F153R mutation indicating that we have a strong tracking between the barcode and codon edits. FIG. 5B depicts the total number of colonies (CFUs) in duplicate experiments that are edited and/or barcoded. The edited CFUs numbers were calculated by extrapolation of the data in FIG. 5A to the total number of CFUs on the plate. The barcoded CFUs numbers were calculated by counting the number of white colonies in a galK screening (site in which barcode is integrated). These data show that the majority of barcoded colonies contained the designed genomic edit.
Example 6—Plasmid Curing for Combinatorial Engineering FIG. 6 depicts an example of combinatorial genome engineering and tracking. Three recursive CREATE plasmids are used, each with a gRNA targeting one of the other markers in this series (indicated by T-lines). During each transformation, an edit and barcode are incorporated into the genome and the previous CREATE plasmid is cured. In this way rapid iterative transformations can be performed to construct either a defined combination of mutations or a combinatorial library to search for improved phenotypes. The recording site is compatible with short read sequencing technologies that allow the fitness of combinations to be tracked across a population. Such an approach allows rapid investigation of genetic epistasis and optimization of phenotypes relevant to basic research or for commercial biological applications.
FIG. 3D and FIG. 3E depict another example of combinatorial genome engineering. With each round of engineering, an editing cassette (blue rectangle in FIG. 3D) is incorporated into the target sequence in the genome (blue star) and a recorder cassette (green rectangle in FIG. 3D) is incorporated into a different target sequence of the genome (green dash in middle panel of FIG. 3D). In this example, each recorder sequence comprises a 15 nucleotide barcode. As shown in the right panel of FIG. 3D, the recorder sequences are each inserted adjacent to the last recorder sequence, despite where the editing cassette was inserted. Each recorder cassette can simultaneously delete a PAM site. After completion of each round of engineering, the engineered cells can be selected and then the inserted mutations can be tracked by sequencing the recorder region that comprises all of the inserted recorder cassettes. By sequencing the starting plasmid library, each editing cassette can be linked or associated with one or more unique barcodes within the recorder cassette. Since each recorder cassette corresponds to the associate editing cassette, then the mutations incorporated by the editing cassettes can be tracked or identified by the sequence of the recorder cassette, or the sequence of the barcodes within the recorder cassette. As is demonstrated in FIG. 3E, by sequencing all of the recorder cassettes or barcodes within the recorder cassettes, each of the inserted mutations can be identified and tracked. The inserted recorder sequences can be referred to as a recorder site, recorder array, or barcode array. As a result, after recursive rounds of engineering, sequencing the barcode array or recorder site allows tracking of the history of genomic editing events in the strain. When the recorder cassettes are inserted in order as depicted, for example, in FIG. 3D, then the barcode array or recorder site can identify the order in which the mutations were inserted as well as what the mutation is.
Example 7— Recursive Engineering Using Iterative CREATE-Recording Engineering Events The example of recursive engineering depicted in FIG. 7A was used for plasmid curing to demonstrate that the design is extremely efficient at eliminating previous vectors (FIG. 7B). Each CREATE plasmid can be positively selected for based on the indicated antibiotics (Trimeth: trimethoprim, Carb: carbenicillin, Tet: tetracycline) and contains a gRNA targeting one of the other antibiotic markers. For example, the reCREATE1 plasmid can be selected for on carbenicillin and encodes a gRNA that will selectively target the trimethoprim resistance gene for destruction. One pass through the carb/tetracycline/trimethoprim antibiotic marker series allows selective incorporation of up to three targeted edits. The recording function would be implemented as illustrated in FIG. 5, but is omitted here for simplicity.
FIG. 7B depicts an example of data from iterative rounds of CREATE engineering. A serial transformation series began with cells transformed with X2cas9 (kan) and the reCREATE1 vector. The spot plating results indicate that curing is 99.99% effective at each transformation step, ensuring highly efficient engineering in each round of transformation. Simultaneous genome editing and plasmid curing in each transformation step with high efficiencies was achieved by introducing the requisite recording and editing CREATE cassettes into recursive vectors as disclosed herein (e.g. FIG. 7B).
Example 8—CREATE Design and Workflow An example overview of CRISPR EnAbled Trackable genome Engineering (CREATE) design workflow is depicted in FIGS. 8A-8B. FIG. 8A shows example anatomy of a CREATE cassette designed for protein engineering. Cassettes encode a spacer (red) along with part of a guide RNA (gRNA) sequence and a designer homology arm (HA) that can template homologous recombination at the genomic cut site. For protein engineering purposes the HA is designed to systematically couple mutations to a specified codon or target site (TS, blue) to a nearby synonymous PAM mutation (SPM, red) to rescue the sequence from Cas9 cleavage and allow highly efficient mutagenesis. The priming sites (P1 and P2, black) are designed to allow multiplexed amplification and cloning of specific subpools from massively parallel array based synthesis. A constitutive promoter (green) drives expression of the gRNA. FIG. 8A further shows a detailed example of HA design for introducing a stop codon at residue 145 in the galK locus. The top sequence shows is of the wt genome with the PAM and TS codon highlighted. The translation sequences are shown to illustrate that the resulting mutant contains a single nonsynonymous mutation at the target site. FIG. 8B shows an example overview of the CREATE workflow. CREATE oligos are synthesized on a microarray and delivered as large pools (104-106 individual library members). These cassettes are amplified and cloned in multiplex with the ability to subpool designs. After introduction of the CREATE plasmids into cells expressing Cas9 mutations are transferred to the genome with high efficiencies. Measurement of the frequency of each plasmid before (fi, t1) and after selection (fi, t2) by deep sequencing provides enrichment scores (Ei) for each CREATE cassette. These scores allow rapid identification of adaptive variants at up to single nucleotide or amino acid resolution for thousands loci in parallel.
Example 9—CREATE Design Validation FIG. 9A depicts an example of the effects of Cas9 activity on transformation and editing efficiencies were measured using no a cassette with a spacer and 120 bp HA targeted to the galK (galK_Y145*_120/17) The total transformants (TT white) produced by this CREATE vector are shown in white and the total number of recombinants (TR) in dark blue. TR is calculated as the product of the editing efficiency and Tt. Asterisks indicate experiments in which recombinants could not be observed by plate based screening. FIG. 9B shows an example of characterization of CREATE cassette HA length and PAM/codon spacing on editing efficiency. All cassettes were designed to introduce a TAA stop at codon 145 in the gene using PAMs at the indicated distance (PAM/codon bottom) from the target codon and variable homology arms lengths (HA, bottom). White and blue bars correspond to uninduced or induced expression of Cas9 under the pBAD promoter using 0.2% arabinose. In the majority of cases the editing efficiency appears to be unaffected by induction suggesting that low amounts of Cas9 due to leaky expression are sufficient for high efficiency editing. FIG. 9C depicts an example of determination of editing efficiency for oligo derived cassettes by sequencing of the genomic loci. The galK_Y145*_120/17 cassette from FIGS. 9A and 9B is shown in white for reference. The bar plot indicates the number of times each genotype was observed by genomic colony sequencing following recombineering with each CREATE cassette. The circular inset indicates the relative position of each gene on the E. coli genome. FIG. 9D depicts distance between SPM and the TS (as exemplified in FIG. 8A) is strongly correlated with editing efficiency (correct edits/total sequences sampled). The galK cassettes with 44 and 59 bp in FIG. 9B were omitted from this analysis. The depicted error bars are derived from N=3 independent replicates of the indicated experiment.
Example 10—Scanning Saturation Mutagenesis of an Essential Chromosomal Gene FIG. 10A-10C depict an example where CREATE was used to generate a full scanning saturation mutagenesis library of the folA gene for identification of mutations that can confer resistance to TMP. The count weighted average enrichment score from two trials of selection is plotted as a function of residue position (right). Cassettes encoding nonsynonymous mutations are shown in gray, and those encoding synonymous mutations in black. Cassettes with enrichment scores greater than 1.8 are highlighted in red and mutations that affect previously reported sites are labeled for reference. The dashed lines indicate enrichment values that are significantly different (p<0.05) from the synonymous dataset as determined by bootstrapping of the confidence intervals. These values are shown as a histogram for reference (middle). Mutations that appear to significantly impact DHFR resistance are highlighted as red spheres to the far right. FIGS. 10D-10F depict example growth analysis of wt (left) F153W (middle) and F153R (right) variants in the indicated range of TMP concentrations (shown right).
Example 11—Reconstruction of ALE Mutation Set and Forward Engineering of Thermotolerant Genotypes FIG. 11A depicts example genomic plots of enrichment scores for CREATE libraries grown at 42.2° C. in minimal media conditions. The innermost plot illustrates the counts of the plasmid library before selection with labels for the top 20 representatives. The outer ring shows the fitness of pooled library variants after growth in minimal media at elevated temperature (42.2° C.). The bars are colored according to log 2 enrichment. Blue bars represent detrimental mutations, red bars represent significantly enriched mutations and gray bars indicate mutations that appear neutral in this assay. The 20 most enriched variants are labeled for reference and labels corresponding to ALE-derived variants are colored red. FIG. 11B shows a histogram of enrichment scores of all library variants (gray), ALE-derived mutants (red) and synonymous mutants (black) under 42.2° C. growth conditions. The dotted gray line indicates significant enrichment scores compared to the synonymous population. The histograms are normalized as a fraction of the total number of variants passing the counting threshold (number indicated in parentheses). Note that 231 of 251 unique nonsynonmous ALE cassettes sampled by this experiment appear to provide significant growth benefits. FIG. 11C depicts enrichment of mutations based on mutational distance from wt. Mutations that require 2 and 3 nucleotide (nt) transitions are exceedingly rare or absent in ALE approaches however we note that the two most enriched clones from the pooled library selection (targeting the Crp regulator) require two nucleotide substitutions and are highlighted at the far right.
Example 12—Genome Scale Mapping of Amino Acid Substitutions for the Study of Antibiotic Resistance and Tolerance FIG. 12A depicts example genomic plots of enrichment (log 2) of library variants in the presence of erythromycin (outer) and rifampicin (middle). The innermost plot illustrates the count distribution of the input plasmids for reference. Coloring and labeling are as in FIG. 11A-11C. FIG. 12B depicts CREATE mutation mapping at the individual amino acid level. CREATE cassettes that introduce bulky side chains to amino acids 1572, S531 and L533 (red) of the RNA polymerase β subunit (rpoB) are highly enriched in the presence of rifampicin from genome wide targeting libraries. FIG. 11C depicts a zoomed in region of the MarA transcription factor bound to its cognate DNA target is shown for reference (PDB ID 1BL0). The wt Q89 residue protrudes away from the DNA binding interface due to unfavorable steric and electrostatic interactions between this side chain and the DNA. The Q89N substitution identified by selection introduces a H-donor and shortens the side chain such that productive H-bonding can occur between this residue and the DNA backbone. Such an interaction likely favors stronger DNA binding and induction of downstream resistance genes. FIG. 12D depicts enrichment plot of genome wide targeting libraries with 10 g/L acetate or 2 g/L furfural respectively. Coloring is the same as in FIG. 11A. FIG. 12E depicts CREATE mapping at a gene level reveals trends at the gene level. Strong enrichment fis metA and fadR targeting mutations in acetate suggests important roles for these genes in acetate tolerance, as depicted in FIG. 12F, same as in the furfural selections depicted in FIG. 12E.
Example 13—CREATE-Enabled Flexible Design Strategies Illustration of example designs compatible with CREATE strategy are depicted in FIGS. 13A-13D. FIG. 13A shows protein engineering applications a silent codon approach is taken (top, see also FIG. 8A-8B). This mutation strategy allows targeted mutagenesis of key protein regions to alter features such as DNA binding, protein-protein interactions, catalysis, or allosteric regulation. Above an illustration of a DNA binding saturation mutagenesis library designed for the global transcription factor Fis designed for this study is illustrated. FIG. 13B shows promoter mutations PAM sites in proximity to a specified transcription start site (TSS) can be disrupted through nucleotide replacement or integration cassettes. To simplify this design procedure used in this study consensus CAP or UP elements were designed for integration at a fixed location relative to the TSS without taking into account possible effects of these mutations may have on proximal genes. FIG. 13C shows an example cassette design for mutagenizing a ribosome binding site (RBS). FIG. 13D depicts an example of a simple deletion design. Points a and b are included to illustrate distance between two sites at the gene deletion locus. In all cases cassette designs disrupt a targeted PAM to allow selective enrichment of the designed mutant.
Example 14—Engineering the Lycopene Pathway FIGS. 14A-14B depict edits made the DMAPP pathway in E. coli which is the precursor to lycopene. Edits were made to the ORF's for 11 genes. Eight edits were designed to improve activity and 3 edits were designed to reduce activity of competitive enzymes. Approximately 10,000 variants within the lycopene pathway were constructed and screened.
Example 15—Cas9 Editing Efficiency Controls FIG. 15 depicts Cas9 editing control experiments. The CREATE galK_120/17 off cassette (relevant edits shown in red at bottom) was transformed into different backgrounds to assess the efficiency of homologous recombination between the CREATE plasmid and the target genome. Red colonies represent unedited (wt) genomic variants and white colonies represent edited variants. Transformation into cells containing only pSIM5 or pSIM5/X2 and dCas9 plasmids exhibited no detectable recombination as indicated by the lack of white colonies. In the presence of active Cas9 (X2-Cas9 far right) we observe high efficiency editing (>80%), indicating the requirements for dsDNA cleavage to achieve high efficiency editing and library coverage.
Example 16—Toxicity of gRNA dsDNA Cleavage in E. coli FIGS. 16A-16C depict experiments testing the toxicity of generating double strand breaks in E. coli. The toxicity of a single gRNA cut in E. coli as observed in control experiments with a gRNA targeting galK (spacer sequence TTAACTTTGCGTAACAACGC (SEQ ID NO: 182)) or folA (spacer sequence GTAATTTTGTATAGAATTTA (SEQ ID NO: 183)). In the absence of a repair template we observe strong killing from the gRNA. Rescue efficiencies of 103-104 are observed upon co-transformation of a single stranded donor oligo indicating the need for a homologous repair template to alleviate this toxicity. b) Toxicity of multiple CREATE edits. The targeted sites are illustrated graphically on the left and at the bottom of the bar graph. A non-targeting gRNA control was used to estimate transformation efficiency based on no edits (far left, no target sites). A CREATE cassette targeting either folA (green) or galK (red) or a combination of the two. Note the multiplicative toxicity in E. coli of having additional gRNAs expressed from the same plasmid. In this scenario there is homologous repair for each site suggesting that off-target gRNA cleavage would be highly lethal. These data suggest that off target cleavage by a CREATE cassette would be selectively removed from the population early in the library construction phase.
FIGS. 16D-16E depicts data from another such cell survival assay. The editing cassette contained a F153R mutation, which leads to temperature sensitivity of the folA gene. The recorder cassette contained a 15 nucleotide barcode designed to disrupt the galK gene, which allows screening of colonies on MacConkey agar plates. In this example, generating two cuts decreased cell survival compared to generating zero or one cut.
FIG. 16F depicts data from a transformation and survival assay comparing a low copy number plasmid (Ec23) expressing Cas9 and a high copy number plasmid (MG) expressing Cas9. Different vectors with distinct editing cassettes were used to target different gene target sites (folA, lacZ, xylA, and rhaA). The recorder cassettes were designed to target different sequences within the galK gene, either site S1, S2, or S3. The recursive vector used had a different vector backbone compared to the others and is part of a 3-vector system designed for iterative engineering that cures the cell of the previous round vector. The data indicates that lower Cas9 expression (Ec23 vector) increases survival and/or transformation efficiency. The decreased Cas9 expression increased transformation efficiency by orders of magnitude in cells undergoing two genomic cuts (editing cassette and recording cassette).
FIG. 16G shows the correlation between editing efficiency and recording efficiency in cells transformed with the low copy number plasmid (Ec23) expressing Cas9 and the high copy number plasmid (MG) expressing Cas9. Editing and recording efficiencies were similar for high (MG) and lower (Ec23) expression of cas9. Ec23 yielded more colonies and had better survival (as shown in FIG. 16E), while maintaining a high efficiency of dual editing (editing cassette and recorder cassette incorporation).
Example 17—CREATE Strategy for Gene Deletion FIG. 17A-D depict an example CREATE strategy for gene deletion. FIG. 17A depicts an example cassette design for deleting 100 bp from the galK ORF. The HA is designed to recombine with regions of homology with the designated spacing, with each 50 bp side of the CREATE HA designed to recombine at the designated site (blue). The PAM/spacer location (red) is proximal to one of the homology arms and is deleted during recombination, allowing selectable enrichment of the deleted segment. FIG. 17B depicts electrophoresis of chromosomal PCR amplicons from clones recombineered with this cassette. FIG. 17C depicts design for 700 bp deletion as in a). FIG. 17D depicts colony PCR of 700 bp deletion cassettes as in FIG. 17B). The asterisks in FIGS. 17B and 17D indicate colonies that appear to have the designed deletion. Note that some clones appear to have bands pertaining to both wt and deletion sizes indicating that chromosome segregation in some of the colonies is incomplete when plated 3 hrs post recombineering.
Example 18—Editing Efficiency Controls by Cotransformation of gRNA and Linear dsDNA Cassettes FIG. 18 depicts effect of PAM distance on editing efficiency using linear dsDNA PCR amplicons and co-transformation with a gRNA. On the left is an illustration of the experiments using PCR amplicons containing a dual (TAATAA) stop codon on one side (asterisk) and a PAM mutation just downstream of the galK gene (gray box) on the other end were co-transformed with a gRNA targeting the downstream galK PAM site. The primers were designed such that the mutations were 40 nt from the end of the amplicon to ensure enough homology for recombination. Data was obtained from these experiments by red/white colony screening. A linear fit to the data is shown at the bottom. Cassettes in which only the PAM mutation is present were included as assay controls were observed to have very low rates of GalK inactivation. These experiments were performed in a BW25113 strain of E. coli in which the mutS gene was knocked out to allow high efficiency editing with double stranded DNA templates. This approach in MG1655 did not achieve high efficiency editing due to the active mutS allele.
Example 19—Library Cloning Analysis and Statistics FIG. 19A depicts reads from an example plasmid library following cloning are shown according to the number of total mismatches between the read and the target design sequence. The majority of plasmids are matches to the correct design. However, there are a large number of 4 base pair indel/mismatch mutants that were observed in this cloned population. FIG. 19B depicts a plot of the mutation profile for the plasmid pool as a function of cassette position. An increase in the mutation frequency is observed near the center of the homology arm (HA) indicating a small error bias in the sequencing or synthesis of this region. We suspect that this is due to the presence of sequences complementary to the spacer element in the gRNA. FIG. 19C depicts a histogram of the distances between the PAM and codon for the CREATE cassettes designed in this study. Large majority (>95%) were within the design constraints tested in FIG. 9A-9D. The small fraction that are beyond 60 bp were made in cases where there was no synonymous PAM mutation within closer proximity. FIG. 19D depicts library coverage from multiplexed cloning of CREATE plasmids. Deep sequencing counts each variant are shown with respect to their position on the genome. The inset shows a histogram of the number of variants having the indicated plasmid counts in the cloned libraries.
Example 20—Precision of CREATE Cassette Tracking of Recombineered Populations FIG. 20A depicts a correlation plot of CREATE cassette read frequencies in the plasmid population prior to Cas9 exposure (x-axis) and after 3 hours post transformation into a Cas9 background. FIG. 20B depicts a correlation plot between replicate recombineering reactions following overnight recovery. The gray lines indicate the line of perfect correlation for reference. R2 and p values were calculated from a linear fit to the data using the Python SciPy statistics package. A counting threshold of 5 for each replicate experiment was applied to the data to filter out noise from each data set.
Example 21—Growth Characteristics of folA Mutations in M9 Minimal Media FIG. 21 depicts growth characteristics of folA mutations in M9 minimal media. While F153R appears to maintain normal growth characteristics the growth rate of the F153W mutation is significantly slower under these conditions, suggesting that these two amino acid substitutions at the same site have very different effects on organismal fitness presumably due to different changes invoked in the stability/dynamics of this protein.
Example 22—Enrichment Profiles for folA CREATE Cassettes in Minimal Media FIG. 22 depicts enrichment profiles for folA CREATE cassettes in minimal media. Cassettes that encode synonymous HA are shown in black and non-synonymous cassettes in gray, the dashed lines indicate enrichment scores with p<0.05 significance compared to the synonymous population mean as estimated from a bootstrap analysis. The enrichment score observed for each mutant cassette at each position in the protein sequence is shown to the left and a histogram of these enrichment scores as a fraction of the total variants to the right. The two populations appear to be largely similar. Conserved residues that are highly deleterious are shown in blue for reference.
Example 23—Validation of Newly Identified acrB Mutations for Improved Solvent and Antibiotic Tolerance FIG. 23A depicts on the left a global overview of AcrB efflux pump. Substrates enter the pump through the openings in the periplasmic space and are extruded via the AcrB/AcrA/TolC complex across the outer membrane and into the extracellular space. Library targeted residues are highlighted by blue spheres for reference and the red dot indicates the region where many of the enriched variants clustered. On the right is a blow up of the loop-helix motif abutting the central funnel where enriched mutations in isobutanol were identified (red and teal spheres), presumably affecting solute transport from the periplasmic space. Mutants targeting the T60 position (teal spheres) was also enriched in the presence of erythromycin. FIG. 23B depicts confirmation of N70D and D73L mutations for tolerance to isobutanol. The N70D mutation in particular appears to improve the final OD to a significant degree. Reconstructed strains were measured for final OD in capped 1.5 mL eppendorf tubes following 48 hours incubation. Error bars are derived from N=3 trials and p-values derived from a one-tailed T-test. FIG. 23C depicts improved growth of the AcrB T60N mutant was observed in inhibitory concentrations of erythromycin (200 μg/mL) and isobutanol (1.2%) in shaking 96 well plate, indicating that this mutation may enhance the efflux activity of this pump towards many compounds. For these experiments CREATE cassette designs were individually synthesized, cloned and sequence verified before recombineering into E. coli MG1655 to reconstruct the mutations and the genomic modifications were sequence verified by colony PCR to confirm the genotype-phenotype association.
Example 24—Benefits of Rational Mutagenesis for Sampling Novel Adaptive Genotypes FIGS. 24A-24D depict the number of variants detected in CREATE experiments involving 500 μg/mL rifampicin (FIG. 24A), 500 μg/mL erythromycin (FIG. 24B), 10 g/L acetate (FIG. 24C), and 2 g/L furfural (FIG. 24D). While naturally evolving systems or error-prone PCR are highly biased towards sampling single nucleotide polymorphisms (e.g. 1 nt mutations, red) these histograms illustrate the potential advantages for rational design approaches that can identify rare or inaccessible mutations (2 and 3 nt, green and blue respectively). For example, the highest fitness solutions appear to be biased toward these rare mutations in rifampicin, erythromycin and furfural selections to varying degrees. These results indicate that procedures such as CREATE should allow more rapid and thorough analysis of fitness improving mutations, in much the same way that computational approaches are being used to improve directed evolution for protein engineering.
Example 25—Reconstruction of Mutations Identified by Erythromycin Selection FIG. 25 depicts reconstructed strains grown in 0.5 mL in capped 1.5 mL eppendorf tubes following 48 hours incubation in the presence of 200 μg/mL erythromycin and final OD measurements assessed. Error bars are derived from N=3 trials. A one tailed T-test was performed on each set of measurements to determine p-values indicated for significance of growth benefit.
Example 26—Validation of Crp S28P Mutation for Furfural or Thermal Tolerance FIG. 26A depicts a crystal structure of the Crp regulatory protein with variants identified by furfural selection highlighted in red (PDB ID 3N4M). A number of the CREATE designs targeting residues near the cyclic-AMP binding site (aa. 28-30, 65) of this regulator were highly enriched in minimal media selections for furfural or thermal tolerance suggesting that these mutations may enhance E. coli growth in minimal media under a variety of stress conditions. FIG. 26B depicts validation the Crp S28P mutant identified in 2 g/L furfural selections in M9 media. This mutant was reconstructed as described for AcrB T60S in Example 23.
Example 27—Genome-Scale Sequence to Activity Relationship Mapping at Single Nucleotide Resolution Advances in DNA synthesis and sequencing have motivated increasingly complex efforts to rationally program genomic modifications on laboratory timescales. Realization of such efforts requires strategies that span the design-build-test forward-engineering cycle by not only precisely and efficiently generating large numbers of mutant designs but also by mapping the effects of these mutations at similar throughputs. CRISPR EnAbled Trackable genome Engineering (CREATE) couples highly efficient CRISPR editing with massively parallel oligomer synthesis to enable trackable precision editing on a genome wide scale. This can be accomplished using synthetic cassettes that link a targeting guide RNA with rationally programmable homologous repair cassettes that can be systematically designed to edit loci across a genome and track their phenotypic effects. We demonstrated the flexibility and ease of use of CREATE for genome engineering by parallel mapping of sequence-activity relationships for applications ranging from site saturation mutagenesis, rational protein engineering, complete residue substitution libraries and reconstruction of prior adaptive laboratory evolution experiments.
Validation of CREATE Cassette Design
In order to realize our engineering objectives we took into account a number of key design considerations to both maximize the editing efficiency as well as distill a complex design process into an easily executable workflow. For example, each CREATE cassette is designed to include both a targeting guide RNA (gRNA) and a homology arm (HA) that introduces rational mutations at the chromosomal cleavage site (e.g. FIG. 8A). The HA encodes both the genomic edit of interest coupled to a synonymous PAM mutation that is designed to abrogate Cas9 cleavage after repair (e.g. FIG. 8B). This arrangement not only ensures that the desired edit can be selectively enriched to high levels by Cas9 but also that the sequences required to guide cleavage and HR are covalently coupled during synthesis and thus delivered simultaneously to the same cell during transformation. The high efficiency editing of CRISPR based selection in E. coli should also ensure a strong correlation between the CREATE plasmid and genomic sequences and allow the plasmid sequence to serve as a trans-acting barcode or proxy for the genomic edit (e.g. FIG. 8C). Assuming that changes in the plasmid frequency under different selective pressures are correlated to their associated genomic edit thereby allows the impact of precise genomic modifications at many loci to be monitored in parallel using a simple downstream sequencing approach to map enriched genotypes on a population scale, analogous to previous genomic tracking methodologies.
To test this concept we first performed control experiments using a CREATE cassette designed to inactivate the galK gene by introducing a single point mutation to convert codon 145 from TAT to a TAA stop codon (e.g. FIG. 8B) using a 120 bp HA. The editing efficiency of this cassette using Cas9 and the nuclease deficient dCas9 control was evaluated using a red/white colony screening assay (e.g. FIG. 8A-B, FIG. 15A-15C). These experiments also indicated that HR between a circular double stranded plasmid and the chromosome is strongly dependent on the Cas9 cleavage as recombination is not observed in the absence of the active enzyme (e.g. FIG. 15A-15D). This is in contrast to single stranded recombineering approaches in which oligonucleotides anneal with high efficiency at the lagging strand of the replication fork. Cas9 also adversely impacts the overall transformation efficiency due to toxicity of dsDNA cleavage in E. coli (e.g. FIG. 9A-9D). This toxicity is further exacerbated when performing CREATE at two sites simultaneously in the same cell (e.g. FIG. 16A-16E); which when combined with the absence of an effective non-homologous end joining pathway strongly supports the fact that off target editing events should be rare within a recombineered library. Additionally, toxicity limits the size of library construction and coverage, however we note that the observed 104-105 variants/μg DNA (e.g. FIG. 9A) is on a scale compatible with current oligo synthesis capabilities (104-5 oligos per order). Thus, we anticipated that using the CREATE synthetic oligo design, we would be able to simultaneously generate ˜105 or more designer mutations at any location in the genome and precisely map such mutations onto a targeted phenotype.
To further characterize how changes in the CREATE cassette design influence the editing efficiency we varied the HA length (80-120 bp) and the distance between the PAM-codon/TS (17-59 bp) (e.g. FIG. 9B). Induction of Cas9 revealed that all of these cassette variants can support high efficiency HR. High efficiency conversion is also observed in the absence of Cas9 induction indicating that low level expression of Cas9, due to a leaky inducible promoter, is sufficient to drive cleavage and HR (e.g. FIG. 9B). To verify that the edits matched our intended design we sequenced the chromosome of randomly chosen clones and found that 71% (27/38) contained a perfect match to the CREATE design, while 26% (10/38) contained only the PAM edit and the remaining 3% (1/38) appeared to be wt escapers. As an additional test of design flexibility performed similar experiments using deletion cassettes that that introduce different sized deletions (e.g. FIG. 17A-17D) and observed similar efficiencies (>70%) indicating that the same design automation and tracking capabilities should readily extend to a variety of design objectives (e.g. FIG. 13A-13D).
High-Throughput Design and Multiplexed Library Construction
To scale the CREATE process for genome-wide applications we developed a custom software to automate cassette design that takes into account the above mentioned criteria to systematically identify a PAM sequence nearest to a target site (TS) of interest and modify it to create a synonymous PAM mutation. This design software is part of a suite of web-based design tools that can be implemented for E. coli and is under further development for other organisms as well as an expanded set of CRISPR-Cas systems. This software platform enables high-throughput rational design of genomic libraries in a format that is compatible with parallelized array based oligo synthesis and simple homology based cloning methods that can be performed in batch for library construction (e.g. FIG. 8B).
Using this design software we generated a total of 52,356 CREATE cassettes for a range of applications where sequence to activity mapping by traditional methods would be time-consuming and prohibitively expensive. Briefly, the library designs included: 1) a complete saturation of the folA gene to map the entire mutational landscape of an essential gene in its chromosomal context 2) saturation mutagenesis of functional residues in 35 global regulators, efflux pumps and metabolic enzymes implicated in a wide range of tolerance and production phenotypes in E. coli 3) a reconstruction of the complete set of nonsynonymous mutations identified by a recent adaptive laboratory evolution (ALE) study of thermotolerance, and 4) promoter engineering libraries designed to incorporate UP elements or CAP binding elements at transcription start sites annotated in RegulonDB (e.g. FIG. 13A-13D).
The pooled oligo libraries were amplified and cloned in parallel and a subset of single variants were isolated to further characterize editing efficiency at different loci (e.g. FIG. 9C). Amplification and sequencing of the genomic loci after transformation with the CREATE plasmids revealed editing efficiencies of 70% on average (106 of 144 clones sampled at seven different loci), with a range of 30% for the metA V20L cassette to 100% for the rpoH_V179H cassette. Interestingly, the differences in editing efficiency for each cassette were highly correlated with the distance between the PAM and target codon (e.g. FIG. 9D), a feature that also appears to affect the ability of linear DNA templates to effectively introduce targeted mutations (e.g. FIG. 18A-18B). This relationship suggests that subsequent CREATE designs should readily increase editing efficiency by optimizing PAM selection criteria. We also note that differences in editing efficiency may reflect detrimental effects of some mutations on organismal fitness (metA is considered an essential gene in most media conditions), and that there may be an upper bound on the number of mutations that can be observed for a particular protein. Finally, these data were obtained outside of any specific selective or screening steps that enrich for chromosomal mutants of interest, and as such demonstrate the ability of this approach to construct mutational libraries.
To further characterize the fidelity of the multiplexed synthesis and cloning procedures we performed deep sequencing on the pooled libraries (e.g. FIG. 19A-D). From 594,998 total reads of the cloned CREATE cassette libraries, 550,152 (92%) passed quality filtering and produced hits against the design database. Of these we observed a perfect match for 34,291 (65%) of the possible unique variants and note that many cassettes that were missing in this initial pool were observed in later selections, suggesting that at the cloning stage we can readily cover the majority of the intended design space. In depth analysis of these reads revealed that 46% of the reads passing quality filter were exact matches to their intended design, with the remainder containing 1-4 bp indels or mismatches, primarily in the HA region near the designed mutation site (e.g. FIG. 19A). The mutational bias in this region suggests that the repetitive spacer elements in the HA and gRNA portions of the cassette may form secondary structures that adversely affect sequencing or synthesis (e.g. FIG. 19B). We note that these variant designs are easily identified via the CREATE plasmid-barcoding strategy, and that in some cases it may be desired to have this added diversity in the generated library. We also observed significant (p<0.05) correlation between variant frequencies from the cloned pools and after overnight recovery following recombineering, as well as between replicate recombineering experiments (e.g. FIG. 20A-20B). These results suggest that well represented variants should be readily tracked by our methodology with a precision similar to previous CRISPR based saturation mutagenesis procedures performed at a single loci.
CREATE Based Protein Engineering
To test the robustness of the CREATE methodology for protein engineering at a single gene level we performed deep-scanning mutagenesis of the essential folA gene. This gene encodes the dihydrofolate reductase (DHFR) enzyme responsible for the production of tetrahydrofolate and the biosynthesis of pyrimidines, purines and nucleic acids. DHFR is also the primary target of the antibiotic trimethoprim (TMP) and other antifolates that are used as antibiotics or chemotherapeutics. The wealth of structural and biochemical data DHFR function and antibiotic resistance make it an ideal model for validation of the approach.
A CREATE library designed to saturate every codon from 2-158 of the DHFR enzyme was recombineered into E. coli MG1655 and allowed to recover overnight. Following recovery ˜109 cells (1 mL saturated culture) was transferred into media containing inhibitory TMP concentrations and allowed to grow for 48 hours. The resulting plasmid populations were then sequenced to assess our ability to capture information at the level of single amino acid substitutions that can confer TMP resistance (e.g. FIG. 10A-10B). Bootstrapped confidence intervals for mutational effect were derived using the enrichment data of the 158 synonymous mutations included in this experiment (e.g. FIG. 10A-10B). Using this criteria, we observed significant (P<0.05) levels of enrichment for 74 substitutions (2.3% of the design space) covering 49 aa positions in the protein. Although this degree of mutational flexibility of an essential enzyme may seem counterintuitive, it supports previous conclusions that this enzyme has not reached its evolutionary optimum and that many mutations that can improve TMP tolerance through enhancement of the endogenous enzymatic activity or alteration of the dynamic folding landscape of this enzyme.
These results also support the fact that we probe more deeply into the mutation space of improved fitness variants using rational mutagenesis strategies. For example, we observed 7 significantly enriched substitutions at position F153 (e.g. FIG. 10A-10B), none of which have been previously identified by error-prone PCR and adaptive laboratory evolution (ALE). To validate these specific mutations, we reconstructed F153R and F153W variants, which had not been previously reported in the literature and spanned a large range of the measured enrichment scale at this position (e.g. FIG. 10D-10F). We confirmed that the highly enriched F153R mutant grows rapidly under a large range of TMP concentrations while the F153W mutant demonstrates growth only at the moderate TMP concentration used in the selection, consistent with their respective enrichment scores (e.g. FIG. 10A-10F). Moreover, 6 of the 7 mutations we identified using CREATE require two nucleotide changes to convert the wt TTT codon to one of the observed amino acids (I: 1 nt,W: 2 nt,D: 2 nt,R: 2 nt,P: 2 nt,M: 2 nt,H: 2 nt). The F153R and F153W mutations also appear to impact the native enzyme activity in distinct ways (e.g. FIG. 21), implying that these substitutions may confer tolerance by altering the enzymatic cycle of this enzyme in distinct manners.
In addition to mapping substitutions that confer TMP resistance, we also attempted to identify substitutions that affect the native activity of DHFR. To do so, we compared the frequencies of each plasmid variant after overnight growth in M9 (e.g. FIG. 22A-22C). In this case, we observed similar overall enrichment profiles for both synonymous and nonsynonymous mutation sets, with very few mutations observed to have significant impact on growth. This unexpected result suggests a need for greater sequencing depth and/or alternate selection strategies to assign high confidence to low fitness variants.
As a separate validation of protein engineering applications, we generated a 4,240 variant library targeting the AcrB multidrug efflux pump in E. coli (e.g. FIG. 23A-23F). This protein acts as a proton exchange pump that exports a wide variety of chemicals including antibiotics, chemical mutagens, and short chain alcohols that are being pursued as next generation biofuels and motivating numerous engineering efforts. The library was designed to target the interior chamber, the exit funnel that channels substrates towards the outer-membrane component of the AcrB/AcrA/TolC complex, and key regions of the transmembrane domain where mutations conferring tolerance to isobutanol and longer chain alcohols have been identified (e.g. FIG. 23A-23C). We then constructed the AcrB CREATE library identically as for the FolA library and grew the library in the presence of 1.2% isobutanol. Sequencing identified multiple mutations to the loop-helix motif adjacent to the central efflux funnel that were significantly enriched, suggesting this substructure may provide a novel target for engineering enhanced efflux activity. Reconstruction of the AcrB N70D and D73L mutations also confirmed the ability of these mutations to enhance overall growth in the presence of this solvent stress (e.g. FIG. 23D).
Parallel Evaluation of Genotype Fitness from Large Scale Adaptation Studies
We next sought to expand our efforts from the single protein scale and validate the use of CREATE at the genome-scale. To do so we chose to reconstruct and map mutations resulting from a prior adaptive laboratory evolution study of E. coli thermal tolerance. ALE has been used extensively as a tool to study the bacterial adaptation in response to a broad range of environmental stressors. However, in the majority of cases the genome undergoes multiple mutations making it difficult to assess the contribution of each mutation to the phenotype in question. Here, we designed and constructed a CREATE library to include all 645 nonsynonymous mutants from the Tenaillon et al ALE experiment and then subjected this library to growth selection in minimal media at 42.2° C. To assess any possible effects that could arise from the synonymous PAM mutation we included redundancy in the design of this library such that each target codon was coupled to two different PAM mutations to provide a 4 fold design redundancy for each nonsynonymous mutation. For calibration purposes the ALE library was pooled with the protein targeting libraries to allow for relative enrichment comparisons from the non-ALE derived libraries as a benchmark (e.g. FIG. 11A-11C). Of the more than 50,000 cassettes in this experiment we observed 405 cassettes from the ALE derived library above the minimal counting threshold, pertaining to 252 unique variants (e.g. FIG. 11B). Of these 346 cassettes (encoding 231 nonsynonymous changes) were significantly enriched compared with the synonymous controls (e.g. FIG. 11B), suggesting that 92% (231/252) of the mutations sampled confer significant selective growth advantages as individual chromosomal mutations, consistent with their fixation during adaptive growth. Additionally we found that 141 mutations from the additional CREATE libraries were also significantly enriched, with 86 of these targeting residues in or around the cAMP binding site of Crp, a central regulator of carbon metabolism. The identification of such a large number of Crp mutants is highly suggestive of a role for Crp in thermal-tolerance in agreement with previous findings.
For each mutant we also calculated the number of mutations required to convert the wt codon to each of the other 19 amino acids (e.g. FIG. 11C). As with folA, we found that highly impactful mutations, such as the crp S28P and L30Y mutations, require more than a single nucleotide substitution and would therefore be inaccessible or exceedingly rare in naturally evolving systems under laboratory timescales. In fact, this seemed to be a recurrent theme across many of the selections we performed (e.g. FIG. 24A-24D) highlighting again the value of synthetic DNA driven search strategies for genomic engineering applications.
High-Throughput Mapping of Selectable Precision Edits on a Genome Wide Scale
To further validate the method for genome-scale mapping and exploration we challenged genome wide targeting libraries with antibiotics or solvents relevant to bioproduction (e.g. FIG. 12A-12F). In the case of selections performed with rifampicin, an antibiotic that inhibits transcription by the RNA polymerase (e.g. FIG. 12A, inner circle) we observed a number of enriched variants that highlighted the robustness of the CREATE approach for atomic resolution mapping. For example, 10 of the top 50 hits identified mutations to residues 1572, L533 and S531 of the RNA polymerase (3 subunit (encoded by rpoB) including variants that form part of the rifampicin binding site (e.g. FIG. 12B). In 6 of the 7 enriched variants the data suggest that a bulky substitution is necessary to sterically hinder 7 rifampicin binding. In addition to the (3-subunit mutations the rifampicin selections enriched a number mutations to the MarA transcriptional activator, whose over-expression due to marR knockout is a well studied aspect of multiple antibiotic resistance (MAR) phenotypes in E. coli. In the DNA bound crystal structure of MarA, Q89 is positioned near the DNA backbone but pointed into solution due to a steric clash between other possible rotamers and nearest phosphate group on the DNA backbone (e.g. FIG. 12C). Modeling of the MarA Q89N and Q89D mutations identified by this selection suggests that shortening the side chain by a single carbon unit may enable new protein-DNA H-bonding interactions and thereby improve the overall MAR induction response.
To compare these results to an antibiotic that interferes with translation we performed another round of selections in the presence of erythromycin (e.g. outer circle FIG. 12A). The enrichment profiles from this selection again highlighted loci previously implicated in resistance to this antibiotic. For example, we observed strong enrichment of 4 different mutations to the AcrB efflux pump which acts as the primary exporter of this drug from the periplasmic space (e.g. FIG. 12A). Interestingly, one of the variants (AcrB T60N) appears at the same residue identified from isobutanol selections (e.g. FIG. 23A-23F). As with the other mutations, reconstruction validated that at least two of these mutations (e.g. T60N in FIG. 23E-23F and D73L in FIG. 25) can significantly improve tolerance to both erythromycin as well as isobutanol isobutanol, further supporting the idea that this motif may provide a useful engineering target for broad range of tolerance phenotypes. In addition to AcrB we also observed enrichment of multiple soxR and rpoS mutants, both of which have been previously implicated in stress tolerance and general antibiotic resistance phenotypes. In total, we observed 136 of the 341 significantly enriched mutations (40%) were identified within the RpoB, MarA, MarR, SoxR, AcrB, or dxs proteins, each of which has extensive prior validation as antibiotic resistance genes.
Finally, we performed selections using furfural or acetate, common components of cellulosic hydrolysate that inhibit bacterial growth under industrial fermentation conditions and are thus the target of many strain engineering efforts (e.g. FIG. 12D-12F). In the presence of high acetate concentrations (10 g/L, e.g. inner plot FIG. 12D) the top 100 ranking mutations were predominated by cassettes targeting the fis, fadR, rho and fnr genes respectively (e.g. FIG. 12E). The Fis, Fnr and FadR regulators are all involved transcriptional regulation of the primary acetate utilization gene acs, and implicated in the so-called “acetate-switch” which allows the cell to effectively scavenge acetate. Knockout of these regulators leads to constitutive expression of the acetate utilization pathways and improved acetate growth phenotypes suggesting that the mutations identified in this study (e.g. FIG. 12E-12F) likely inhibit these regulatory functions by destabilizing their respective protein targets.
In contrast to the weak acid tolerance of acetate, the enrichment profiles obtained the presence of growth inhibiting concentrations of furfural (2 g/L) were significantly different with the most frequently observed mutations targeting the oxidative stress response regulator rpoS (e.g. FIG. 12F). Furfural growth inhibition is thought to occur through depletion of cellular NADPH pools, an important cofactor in the prevention of oxidative stress and anabolic pathways for cell growth. In line with our findings, previous studies of RpoS have demonstrated that inactive alleles are favored in such nutrient depleted scenarios. Interestingly, we also observed some of the same mutations in crp that were observed in the 42.2° C. selections (e.g. FIGS. 11A and 11C) and upon reconstruction confirmed that the Crp S28P mutant can substantially improve growth in the presence of furfural (e.g. FIG. 26A-26B). We also found that this selection uniquely enriched for variants of the PntA transhydrogenase, a membrane bound transhydrogenase that transfers hydride ions from NADH to NADP+ to maintain sufficient pools for anabolism. A mutation to I258A in close proximity to the substrate binding cleft may therefore impart enhanced NADPH production.
Collectively, these selections validate the CREATE strategy by demonstrating the ability to map known associations as well as highlight power of this method for rapid mapping of novel mutations to traits of interest. It is also important to note that in contrast to the most other functional genomics technologies that mainly identify loss of function mutations, the ability to perform such broad scale scanning mutagenesis opens the door for more general genomic searches that can also identify novel gain of function mutations.
In this work we have demonstrated that CREATE allows parallel mapping of tens of thousands of amino acid and promoter mutations in a single experiment. The construction, selection, and mapping of >50,000 genome-wide mutations (e.g. FIGS. 11A-11C and 12A-12F) can in some examples be accomplished in 1-2 weeks by a single researcher, offering orders of magnitude improvement in economics, throughput, and target scale over the current state of the art methods in synthetic biology. Importantly, the ability to track the enrichment of library variants allows multiplex sequence to activity mapping by a simple PCR based workflow using just a single set of primers as opposed to more complicated downstream sequencing approaches that are limited to a few dozen loci. In addition, the ability to map the effects of single nucleotide or amino acid level variation in coding regions or promoters allows CREATE to address a considerably more diverse set of design objectives than previous high-throughput genomic technologies such as trackable multiplexed recombineering (TRMR) or Tn-seq approaches that are limited to gene resolution analysis. Such capabilities enable new paradigms for deciphering gene function and engineering cellular traits including workflows in which iterative rounds of CREATE could be implemented to perform design-driven genome engineering and address a broad range of ambitions.
Notably, as a further distinction from prior approaches, the high efficiency mutagenesis (e.g. FIG. 9A-9D) reported in this work was not only an order of magnitude improved but was also achieved in a wild type MG1655 strain in which all of the native DNA repair pathways are intact. The majority of previously reported recombineering efforts in E. coli have used single-stranded oligo engineering which requires deletion of the mismatch repair genes or chemically modified oligonucleotides to achieve mutagenesis at 1-30% efficiency. The combination of plasmid based homologous recombination substrates and Cas9 dsDNA cleavage appears to circumvent these requirements (e.g. FIG. 13A-13D and FIG. 9A-9D), eliminating the need for specialized genetic modifications outside of the Cas9 and k-RED genes to perform efficient editing and tracking on a population scale (e.g. FIG. 9A-9D). This fact alongside the broad utility of CRISPR editing suggests that the CREATE approach will readily port to a wide range of microorganisms such as Saccharomyces cerevisiae and other recombinogenic bacteria for which high-efficiency transformation protocols are available. The CREATE strategy should also be compatible with a wide range of CRISPR/Cas systems using similar automation approaches to design and tracking. Extension of this methodology to higher eukaryotes however will require the development of strategies to overcome non-homologous end-joining as well as alternative tracking systems that can stably replicate.
The CREATE strategy provides a streamlined approach for sequence to activity mapping and directed evolution by integrating multiplexed oligo synthesis, CRISPR-CAS editing, and high-throughput sequencing.
Example 28—Genome-Scale Sequence to Activity Relationship Mapping at Single Nucleotide Resolution, Additional Examples Possible Effects of Inconsistent Mapping of Plasmid Barcode to Genomic Edit
We note that the initial CREATE library included designs that we would expect to have low confidence mapping between the plasmid barcode and the genomic edit (as explained primarily by distance between the PAM and target mutation in the CREATE cassette, see FIG. 2d). We describe below the various scenarios that may arise in the fraction of cases where the plasmid tracking may lead to erroneous conclusions regarding a genomic variant. A few things to note in evaluating these scenarios include i) the plasmid cassette should have minimal or no functional influence relative to the genomic edit, ii) the genomic loci will only be either the WT sequence or the sequence from the editing cassette that we obtain via sequencing, and iii) offsite editing is highly unlikely given the toxicity of CRISPR-Cas editing of multiple sites (e.g. FIG. 16A-16E) or when performed in the absence of an added editing-repair template. Finally, we note that the use of replicate experiments and deeper sequencing can also address these issues.
Tracking of High Fitness Variants (Positive Enrichment Tracking)
In cases where there is a strong selective advantage for the genomic modification (and thus the associated plasmid) we will only observe cells with the edit in the chromosome post selection. Thus, this is almost always a true positive particularly when selection times are short, thus limiting the possibility of random mutations due to replication error sweeping the population. While this phenomenon may lead to a quantitative underestimation of the true fitness of a mutation due to an enrichment profile that represents the convolution of modified and wt fitness, it will not produce false positives. Moreover, the use of replicated experiments and/or longer selections can also address this potential issue and eliminate erroneous conclusions regarding a mutations impact on fitness.
Tracking of Low Fitness Variants (Negative Enrichment Tracking)
In cases where the encoded mutation has a negative fitness contribution but is linked to a PAM only or unmodified chromosome we would incorrectly overestimate the fitness of the mutant and assume that it is closer to wt, especially for longer selection times (e.g. see FIG. 22A-22C). However, any deep sequencing approach must deal with similar limitations due to the lack of information regarding such mutations following selection and the problems associated with counting statistics in these scenarios. Moreover, we would note that this scenario is only relevant to the subset of truly negative fitness mutants (which should be 10-20% based on historic directed evolution and ALE data) within the unedited fraction (˜30%) and that remain in the unedited fraction in multiple replicate transformations. In other words, it is a small percentage (4-5%) scenario that can be detected and/or addressed through replicate transformations where one would observe inconsistencies in the particular mutant showing up occasionally with WT fitness.
Incomplete Coverage
In cases where a variant is not present in the initial population (due to both low transformation efficiency and low editing efficiency) a couple of scenarios could arise. As implied by the points above, if the mutation is beneficial one could falsely conclude that it does not confer a fitness advantage, and if it is truly deleterious it also could be incorrectly assigned a neutral fitness score. This appears to be encountered sometimes in this work and impacts both the error associated with replicate measurements and our ability to distinguish low fitness variants from a synonymous control. However, our ability to identify beneficial mutants is robust despite these issues as evidenced by our ability to readily identify novel and previously validated mutations. Strategies to address this by overcoming Cas9 toxicity and improving recombineering efficiencies hold promise to largely eliminate such problems. Furthermore, increasing the number of replicates, increasing sequencing depth, and/or improving the library coverage by performing larger scale transformation also can help to address these issues.
Off Target gRNA Cleavage
Off target gRNA cleavage should be rare in E. coli due to the relatively small size of its genome (4 Mb), and thus lack of (non-targeted) regions of homology to the CREATE cassette. Moreover, the toxicity of gRNAs in the presence of Cas9 (e.g. FIG. 9A) ensures that cells survival is compromised in E. coli due to dsDNA breaks. Each additional cut introduced into E. coli appears to incur multiplicative toxicity effects, even when homologous repair templates are provided for each cut site (e.g. FIG. 16A-16E). This toxicity effect would be further exacerbated by the absence of a repair template to guide HR (e.g. FIG. 16A-16E), as would be the case for an off-target cleavage event from a single gRNA targeting two sites but containing only a single HA.
Random Off Target Mutagenesis (Evolution)
The probability that a CREATE variant is strongly enriched due to an off target mutation even is highly improbable due to 2 factors: 1) the toxicity effect for the reasons stated above and 2) the low mutation rates of MG1655 or other mutation repair proficient strains compared with the mutagenesis rates of CREATE, particularly in multiple replicates of selection. We also have validated that we can transfer the plasmid pool back into a naive parental background and rapidly verify the enrichment of fitness improving CREATE plasmids from the initial population. Like replicate data, this allows us to decouple each CREATE plasmid from the potential of background mutations that would interfere with our analysis. These factors simplify the assumptions made during our analysis, the validity of which is supported both by externally and internally validated genotypes that were identified during this work.
Possible Effects of Synonymous Mutations
Synonymous mutations (e.g. in the PAM region) can confer unexpected effects on phenotype. We have controlled for this in a number of manners. In every experiment we included an internal control that consists of a library of synonymous mutations ( 1/20 at each codon or 5% of total input), each of which samples different PAM and codon combinations and thus give us an idea of the range of possible effects we may have on a gene by measuring the enrichment profile of many synonymous changes. Using this population as a control we can accurately identify significant fitness changes at the resolution of single amino acids as the work suggests. We can also control for this effect by utilizing redundant sampling approaches where a site is coupled to multiple PAM mutations similar to what was done for the ALE study described herein.
CREATE Library Design Considerations
A variety of design principles were implemented in the gene targeting libraries described in some work disclosed herein. For example, the folA library (3140 cassettes) was designed to be an unbiased, exploratory library for full single site saturation mutagenesis and sequence activity. However, for the majority of the genes we sought to maximize the probability of interesting genotypes by choosing to focus the diversity of sites most likely to have a functional impact on the targeted protein (e.g. DNA binding sites, active sites, regions identified as mutational hotspots by previous selections). The sites that were included in these library designs were selected based on information deposited in databases including Ecocyc (biocyc.org/), Uniprot (uniprot.org/), and the PDB (rcsb.org/pdb) as well as relevant literature citations that identified residues or regions of interest using directed evolution approaches. The Uniprot and Ecocyc databases provide manually curated sequence features that indicate mutational effects and important domains of each protein. In cases where there was enough structural information to model ligand or DNA binding sites the relevant crystal structures were loaded into Pymol and manual residue selections were made and exported as numerical lists. For promoter libraries we took into account the spacing of these sites relative to the transcription start site and the canonical recognition sequence of either the CRP binding site (AAATGTGAtctagaTCACATTT located between −72 and −40 relative to the transcription start site) or the UP element (AAAATTTTTTTTCAAAAGTA (SEQ ID NO: 185) −60 from the transcription start site) that directly recruit the alpha subunit of the RNA polymerase. These sequences were designed to integrate at these positions relative to the publicly available transcriptional start site annotations in RegulonDB using a variation of the automated CREATE design software designed for protein targeting (e.g. FIG. 13A-13D). These cassettes were made with the intent of assessing the effects of gene dosage and regulation on fitness. Finally, we designed a library to reconstruct all of the 645 non-synonymous mutations targeting 197 genes that were identified by a comprehensive ALE experiment in which the complete genomes of 115 isolates were sequenced after a year of adaptation to growth at elevated temperature (e.g. 42.2° C.). In all, we designed 52,356 oligomers, with 48,080 intended to saturate 2404 codon positions across 35 genes, 2,550 oligos were made for regenerating the ALE mutations, 379 UP promoter mutants and 772 CAP promoter mutations in a manner that would allow simultaneous sequence to activity relationship mapping.
Cassette Design and Automation Principles
Based on the control experiments with galK (e.g. FIG. 9A-9D) and current maximal commercial synthesis length constraints (200 bp from Agilent) we developed a general design for each CREATE cassette (e.g. FIG. 8A-8B).
Design of the CREATE cassettes was automated using custom Python scripts. The basic algorithm takes a gene sequence, a list of target residues, and a list of codons as inputs. The gene sequence is searched for all available PAM sites with the corresponding spacer sequence. This list is then sorted according to relative proximity to the targeted codon position. For each PAM site in the initial list the algorithm checks for synonymous mutations that can be made in-frame that also directly disrupt the PAM site, in the event that this condition is met the algorithm proceeds to making the prescribed codon change and designing the full CREATE cassette with the accompanying spacer and iterates for each input codon and position respectively. For each PAM mutation, all possible synonymous codon substitutions are checked before proceeding to the next PAM site. For the codon saturation libraries in this study we chose the most frequent codons (genscript.com/cgi-bin/tools/codon_freq_table) for each designed amino acid substitution according to the E. coli usage statistics. The script can be run rapidly on a laptop computer and was used to generate the full design of these libraries in <10 minutes. The algorithm used in this study was designed to make the most conservative mutations possible by sometimes using only the PAM as the selectable mutation marker.
Plasmids
The X2-cas9 broad host range vector was constructed by amplifying the cas9 gene from genomic S. pyogenes DNA into the pBTBX2 backbone (Lucigen). A vector map and sequence of this vector and the galK_Y145*_120/17 CREATE cassette are provided at the following locations: benchling.com/s/3c941j/edit; benchling.com/s/xRBDwcMy/edit.
The editing experiments performed in some of this work employed the X2-cas9 vector in combination with the pSIM5 vector (redrecombineering.ncifcrf.gov/strains-plasmids.html) to achieve the reported efficiencies.
Recombineering of CREATE Libraries
Genomic libraries were prepared by transforming CREATE plasmid libraries into a wildtype E. coli MG1655 strain carrying the temperature sensitive pSIM5 plasmid (lambda RED) and a broad host range plasmid containing an inducible cas9 gene from cloned from S. pyogenes genomic DNA into the pBTBX-2 backbone (X2cas9, e.g. FIG. 15A-15D). pSIM5 was induced for 15 min at 42° C. followed by chilling on ice for 15 min. The cells were washed 3 times with ⅕ the initial culture volume of ddH2O (e.g. 10 mL washes for 50 mL culture). Following electroporation the cells were recovered in LB+0.4% arabinose to induce Cas9. The cells were recovered 1-2 hrs before spot plating to determine library coverage and transferred to a 10× volume for overnight recovery in LB+0.4% arabinose+50 μg/mL kanamycin+100 μg/mL carbenicillin. Saturated overnight cultures were pelleted and resuspended in 5 mL of LB. 1 mL was used to make glycerol stocks and the other 1 mL washed with the appropriate selection media before proceeding with selection.
For the control experiments with galK we used CREATE cassettes designed to convert Y145 (TAT) into a stop codon (TAA) with a single point mutation at this position and a second point mutation to make a synonymous mutation that abolishes the targeted PAM site (e.g. FIG. 8B and FIG. 13A-13D). Editing efficiencies (e.g. FIG. 13A-13D and FIG. 9A-9B) were estimated using red/white plate based screening on 1% galactose supplemented MacConkey agar as previously described.
Selection Procedures
Following overnight recovery, the cells were harvested by pelleting and resuspension in fresh selection media. All selections were performed in shake flask and inoculated at an initial OD600 of 0.1. Three serial dilutions (48-96 hrs depending on growth rates in the target condition) were carried out for each selection by transferring 1/100th the media volume after the cultures reached stationary phase. The 42° C. selections were performed in M9 media+0.2% glucose to mimic low carbon availability from the initial adaptation. Antibiotic selections were carried out in LB+500 μg/mL rifampicin or erythromycin to ensure stringent selection. The solvent selections were performed in M9+0.4% glucose and either 10 g/L acetate (unbuffered) or 2 g/L furfural. Selections were harvested by pelleting 1 mL of the final culture and the cell pellet was boiled in 100 μL TE buffer to preserve both the plasmid and the genomic DNA for further desired analyses.
Library Preparation and Sequencing
Custom Illumina compatible primers were designed to allow a single amplification step from the CREATE plasmid and assignment of experimental reads using barcodes. The CREATE cassettes were amplified directly from the plasmid sequences of boiled cell lysates using 20 cycles of PCR with the Phusion (NEB) polymerase using 60° C. annealing and 1:30 minute extension times. As in the cloning procedure a minimal number of PCR cycles was maintained to prevent accumulation of mutations and recombined CREATE cassettes that were observed when an excessive number of PCR cycles was implemented (e.g. >25-30). Amplified fragments were verified and quantified by 1% agarose gel electrophoresis and pooled according to the desired read depth for each sample. The pooled library was cleaned using Qiaquick PCR cleanup kit and processed for NGS using standard Illumina preparation kits. The Illumina sequencing and sample preparation were performed with the primers.
Preprocessing of High-Throughput Sequencing and Count Generation
Paired-end Illumina sequencing reads were sorted according to the golay barcode index with allowance of up to 3 mismatches then merged using the usearch-fastq_merge algorithm. Sorted reads were then matched against the database of designed CREATE cassettes using the usearch global algorithm at an identity threshold of 90% allowing up to 60 possible hits for each read. The resulting hits were further sorted according to percent identity and read assignment was made using the best matching CREATE cassette design at a final cutoff 98% identity to the initial design. It should be noted that this read assignment strategy attempts to identify correlations between the designed genotypes and may therefore miss other important features that arise due to mutations that could occur during the experimental procedure. This approach was taken both to simplify data analysis as well as evaluate the ‘forward’ design and annotation procedure and it's ability to accurately identify meaningful genetic phenomena.
Data Analysis and Fitness Calculation
Enrichment scores (or absolute fitness scores) were calculated as the log 2 enrichment score using the following equation:
where Fx,f is the frequency of cassette X at the final time point and Fx,i is the initial frequency of cassette X and W is the absolute fitness of each variant. Frequencies were determined by dividing the read counts for each variant by the total experimental counts including those that were lost to filtering. Each selection was performed in duplicate and the count weighted average of the two measurements was used to infer the average fitness score of each mutation as follows:
These scores were used to rank and assess the fitness contributions of each mutation under the various selection pressures investigated. For all selections we took average absolute fitness scores for all of the synonymous mutants as a composite measure of the average growth rate. Absolute enrichment scores were considered significant if the mutant enrichment was at least +/−2*σ (e.g. p=0.05 assuming a normal distribution) of the wild-type value. We performed two replicates of each selection reported in this study to derive these figures and applied a cutoff threshold of 10 across the replicate experiments for inclusion in each analysis.
For every codon targeted our designs also included a synonymous variant to provide an internal experimental control. Thus 5% of the protein targeting cassettes encoded synonymous mutations that allow us to estimate confidence intervals for mutation effects using custom Python bootstrapping scripts. The enrichment data for each experiment was resampled with replacement 20000 to obtain 95% confidence interval estimations that were used to infer statistical significance of enrichment scores for each analysis presented in the manuscript.
Mutant Reconstructions and Growth Measurements
The AcrB T60N and Crp S28P and FolA F153R/W CREATE cassettes were ordered as separate gblocks from IDT, cloned and sequence verified. Each cassette was transformed into MG1655 and colony screened to identify a clone with the designed genomic edit. These strains (e.g. FIG. 21 and FIG. 22A-22C) were then subjected to the growth conditions from the pooled library selection as indicated. The growth curves were taken in triplicate for each condition in 100 μL in a 96 well plate reader set to measure absorbance at 600 nm. The plate was covered and water added to empty wells to reduce evaporation during the growth.
Software and Figure Generation
Circle plots were generated using Circos v0.67. Plots were generated in Python 2.7 using the matplotlib plotting libraries and figures were made using Adobe Illustrator CS5. Entropy scores for the FolA (FIG. 10A) were determined using the ProDy Python package and the Pfam accession PF00186 representative proteome alignment RP35.
Figures of the protein libraries and high fitness mutations were made using The PyMol Molecular Graphics System, Schrodinger, LLC. The following are the proteins and PDBs used in the figure generation: AcrB (3W9H, 4K7Q, 3AOC), Fis (3JR9), Ihf (1IHF), RNA polymerase (4KMU, 4IGC), Crp (3N4M), MarA (1BLO), and SoxR (2ZHG).
Example 29: Testing Edit-Barcode Correlation A strain expressing a low copy number plasmid (Ec23) which is a Cas9-pSIM5 dual vector, was tested using different gene editing cassettes (lacZ, xylA, and rhaA) and recorder cassettes with different barcodes and insertion sites (galK site 1, galK site 2, and galK site 3) (Summarized in FIG. 27A). The possible outcomes are depicted in FIG. 27B. Pre-selection, all combinations of edit/barcode/WT are possible. After selection, edits cells could be enriched whether they are barcoded or not in this experimental design.
The transformations were plated on selective media that allowed for enrichment of cells containing the gene edits. 30 colonies from each combination transformation were sequenced to determine if they contained the desired barcode.
FIG. 27C shows the results from the sequencing data. Two of the edit/barcode combinations were found in 100% of the tested colonies (30/30 colonies), and the other edit/barcode combination transformation was found in approximately 97% of tested colonies (29/30 colonies). The single colony that was not properly engineered contained the gene edit, but not the barcode.
Overall, 89 out of 90 tested colonies has the designed gene edit and barcode.
Example 30: Selectable Recording When a barcode is not selected for, it allows for enrichment of non-barcoded cells even if the corresponding gene edit is incorporated and selected for. FIG. 28 depicts an example strategy for selecting for the recording event (e.g., incorporation of the barcode by the recorder cassette), in addition to selecting for the editing cassette incorporation, thereby increasing the efficiency of recovering cells that have been both edited and barcoded.
As depicted in FIG. 28, sequences S0, S1, S2, etc. are designed to be targeted by the guide RNA associated with the recorder cassette of the next round. In the depicted example, in the first round of engineering, a PAM mutation, a barcode, S1 site, and regulatory elementary necessary to turn on a selectable marker are incorporated into the S0 site in the target region. This turns on the TetR selectable marker and allows for enrichment of barcoded mutants variants with the S1 site that have the first round PAM site deleted. In the second round of engineering, a new recorder cassette comprising a second PAM mutation, a second barcode, a S2 site, and a mutation that turns off the selectable marker is incorporated into the S1 site from the previous round. This allows for counter-selection of variants that have incorporated the second barcode and S2 site. The subsequent rounds continue to flip the selectable marker between an on and off state and using selection or counter-selection respectively to enrich the desired variants. The recorder cassette from each round is designed to incorporate into a unique sequence (e.g., S0, S1, etc.) that was incorporated in the previous round. This ensures that the last round of barcoding was successful so that all desired engineering steps are contained in the final product. The incorporation of PAM mutations at each step also helps ensure that the desired barcoded variants are selected for since cells having the unmodified PAM sequences will be killed as they can't escape CRISPR enzyme cleavage.
This strategy uses multiple methods to increase the efficiency of isolating desired variants that contain all of the engineered edits from each round of engineering. The PAM mutation, selectable marker switch, and unique landing site incorporated in each round separately increase efficiency and together increase efficiency as well. These tools allow for selection of each recording round and allow design of highly active recording guide RNAs. An array of equally spaced (or not equally spaced, depending on the design) barcodes is generated and facilitates downstream analysis such as sequencing the barcode array to determine which corresponding edits are incorporated throughout the genome.
FIG. 29 depicts an experimental design to test the selectable recorder strategy described above. A plasmid (pREC1) containing an editing cassette and a recorder cassette was transformed into cells. The editing cassette either contained a non-targeting editing cassette, or a mutation that incorporated a mutation (not TS) or a temperature sensitive mutation (TS) into a target gene. The recorder cassette was designed to incorporate into the S0 site in the target gene that originally had the tetR selectable marker turned off. The recorder cassette also contained a PAM mutation that deleted the S0 PAM site, first barcode (BC1), a unique S1 site for the subsequent engineering round recording cassette to incorporate into, and a corrective mutation that will turn on the TetR selectable marker. A guide RNA on the recorder cassette that targets a PAM site in the S0 site (S0-gRNA) allows a CRISPR enzyme, in this case Cas9, to cleave the S0 site. The recorder cassette recombines into the cleaved S0 site. The PAM mutation is incorporated, which means the S0-gRNA can no longer target the S0 site, thereby killing WT cells and enriching for cells that received the barcode. The TetR selectable marker was also turned on, allowing further selection of the barcoded variant.
The data in FIGS. 30A and 30B show the results from the experiment described above and depicted in FIG. 29. Of the Tet Resistant colonies that were recovered from the transformation and engineering round, 16 were sequence and determined to all contain the designed barcode (FIG. 30A). FIG. 30B shows that the control cells that did not contain the recorder target site (non-target) did not survive the presence of Tet, while cells that contained the target site were successfully barcoded as evidences by the turning on of TetR, allowing cells to be selected on Tet containing media. The Tet resistant colonies were confirmed at the genomic site to have TetR gene turned on. These data showed that selectable recording was successful.
Example 31: Expression of MAD Nucleases Wild-type nucleic acid sequences for MAD1-MAD20 include SEQ ID NOs 21-40, respectively. These MAD nucleases were codon optimized for expression in E. coli and the codon optimized sequences are listed as SEQ ID NO: 41-60, respectively (summarized in Table 2). Codon optimized MAD1-MAD20 were cloned into an expression construct comprising a constitutive or inducible promoter (e.g., T7 promoter SEQ ID NO: 83, or pBAD promoter SEQ ID NO: 81 or SEQ ID NO: 82) and an optional 6×-His tag (SEQ ID NO: 186). The generated MAD1-MAD20 expression constructs are provided as SEQ ID NOs: 61-80, respectively.
TABLE 2
WT Codon
MAD nucleic optimized
nu- acid nucleic acid Amino acid Expression
clease sequence sequence sequence constructs
MAD1 SEQ ID SEQ ID NO: 41 SEQ ID NO: 1 SEQ ID NO: 61
NO: 21
MAD2 SEQ ID SEQ ID NO: 42 SEQ ID NO: 2 SEQ ID NO: 62
NO: 22
MAD3 SEQ ID SEQ ID NO: 43 SEQ ID NO: 3 SEQ ID NO: 63
NO: 23
MAD4 SEQ ID SEQ ID NO: 44 SEQ ID NO: 4 SEQ ID NO: 64
NO: 24
MAD5 SEQ ID SEQ ID NO: 45 SEQ ID NO: 5 SEQ ID NO: 65
NO: 25
MAD6 SEQ ID SEQ ID NO: 46 SEQ ID NO: 6 SEQ ID NO: 66
NO: 26
MAD7 SEQ ID SEQ ID NO: 47 SEQ ID NO: 7 SEQ ID NO: 67
NO: 27
MAD8 SEQ ID SEQ ID NO: 48 SEQ ID NO: 8 SEQ ID NO: 68
NO: 28
MAD9 SEQ ID SEQ ID NO: 49 SEQ ID NO: 9 SEQ ID NO: 69
NO: 29
MAD10 SEQ ID SEQ ID NO: 50 SEQ ID NO: 10 SEQ ID NO: 70
NO: 30
MAD11 SEQ ID SEQ ID NO: 51 SEQ ID NO: 11 SEQ ID NO: 71
NO: 31
MAD12 SEQ ID SEQ ID NO: 52 SEQ ID NO: 12 SEQ ID NO: 72
NO: 32
MAD13 SEQ ID SEQ ID NO: 53 SEQ ID NO: 13 SEQ ID NO: 73
NO: 33
MAD14 SEQ ID SEQ ID NO: 54 SEQ ID NO: 14 SEQ ID NO: 74
NO: 34
MAD15 SEQ ID SEQ ID NO: 55 SEQ ID NO: 15 SEQ ID NO: 75
NO: 35
MAD16 SEQ ID SEQ ID NO: 56 SEQ ID NO: 16 SEQ ID NO: 76
NO: 36
MAD17 SEQ ID SEQ ID NO: 57 SEQ ID NO: 17 SEQ ID NO: 77
NO: 37
MAD18 SEQ ID SEQ ID NO: 58 SEQ ID NO: 18 SEQ ID NO: 78
NO: 38
MAD19 SEQ ID SEQ ID NO: 59 SEQ ID NO: 19 SEQ ID NO: 79
NO: 39
MAD20 SEQ ID SEQ ID NO: 60 SEQ ID NO: 20 SEQ ID NO: 80
NO: 40
Example 32: MAD2 and MAD7 Nucleases MAD2 and MAD7 nucleases are nucleic acid-guided nuclease that can be used in the methods disclosed herein. Nucleases Mad2 (SEQ ID NO: 2) and Mad 7 (SEQ ID NO: 7) were cloned and transformed into cells. Editing cassettes designed to mutate a target site in a galK gene were designed with mutations, which allowed for white/red screening of successfully editing colonies. The editing cassettes also encoded a guide nucleic acid designed to target galK. The editing cassettes were transformed into E. coli cells expressing MAD2, MAD7, or Cas9. FIG. 31A shows the editing efficiency of Mad2 and Mad7 compared to Cas9 (SEQ ID NO: 110). FIG. 31B shows the transformation efficiency as evidenced by cell survival rates. In this example, the guide nucleic acid used with MAD2 and MAD7 comprised a scaffold-12 sequence and a guide sequence targeting galK. The guide nucleic acid used with Cas9 comprised a sequence compatible with the S. pyogenes Cas9.
FIG. 32 and Table 3 show more examples of gene editing using the MAD2 nuclease. In this experiment, different guide nucleic acid sequences were tested. The guide sequence of the guide nucleic acids targeted the galK gene as described above. The scaffold sequence of the guide nucleic acids were one of various sequences tested as indicated. Guide nucleic acids with scaffold-5, scaffold-10, scaffold-11, and scaffold-12 were able to form functional complexes with MAD2.
FIG. 33 and Table 4 show more examples of gene editing using the MAD7 nuclease. In this experiment, different guide nucleic acid sequences were tested. The guide sequence of the guide nucleic acids targeted the galK gene as described above. The scaffold sequence of the guide nucleic acids were one of various sequences tested as indicated. Guide nucleic acids with scaffold-10, scaffold-11, and scaffold-12 (e.g., FIG. 31A) were able to form functional complexes with MAD7. Amino acid sequences are provided in Table 2 and scaffolding sequences are provided in Table 3 and Table 4. Table 3 and Table 4 also provided the designed mutations in the editing cassettes that were used to mutate the galK target gene.
Further details and characterization of MAD2, MAD7, and other MAD nucleases are described in U.S. application Ser. No. 15/631,989, filed Jun. 23, 2017, and U.S. application Ser. No. 15/632,001, filed Jun. 23, 2017, each of which are incorporated herein in their entirety.
TABLE 3
Nucleic Editing
acid-guided Guide nucleic acid sequence Target
# nuclease scaffold sequence mutation gene
1 MAD2 Scaffold-12; SEQ ID NO: 95 N89KpnI galK
2 MAD2 Scaffold-10; SEQ ID NO: 93 L80** galK
3 MAD2 Scaffold-5; SEQ ID NO: 88 L80** galK
4 MAD2 Scaffold-12; SEQ ID NO: 95 D70KpnI galK
5 MAD2 Scaffold-12; SEQ ID NO: 95 Y145** galK
6 MAD2 Scaffold-11; SEQ ID NO: 94 Y145** galK
7 MAD2 Scaffold-10; SEQ ID NO: 93 Y145** galK
8 MAD2 Scaffold-12; SEQ ID NO: 95 L10KpnI galK
9 MAD2 Scaffold-11; SEQ ID NO: 94 L80** galK
10 SpCas9 S. pyogenese gRNA Y145** galK
11 MAD2 Scaffold-2; SEQ ID NO: 85 Y145** galK
12 MAD2 Scaffold-4; SEQ ID NO: 87 Y145** galK
13 MAD2 Scaffold-1; SEQ ID NO: 84 L80** galK
14 MAD2 Scaffold-13; SEQ ID NO: 96 Y145** galK
TABLE 4
Nucleic Editing
acid-guided Guide nucleic acid sequence Target
# nuclease scaffold sequence mutation gene
1 MAD7 Scaffold-1; SEQ ID NO: 84 L80** galK
2 MAD7 Scaffold-2; SEQ ID NO: 85 Y145** galK
3 MAD7 Scaffold-4; SEQ ID NO: 87 Y145** galK
4 MAD7 Scaffold-10; SEQ ID NO: 93 Y145** galK
5 MAD7 Scaffold-11; SEQ ID NO: 95 L80** galK
While preferred embodiments of the present invention have been shown and described herein, it will be obvious to those skilled in the art that such embodiments are provided by way of example only. Numerous variations, changes, and substitutions will now occur to those skilled in the art without departing from the invention. It should be understood that various alternatives to the embodiments of the invention described herein may be employed in practicing the invention. It is intended that the following claims define the scope of the invention and that methods and structures within the scope of these claims and their equivalents be covered thereby.
SEQUENCE LISTING TABLE 5
SEQ
ID
NO: Sequence
SEQ MGKMYYLGLDIGTNSVGYAVTDPSYHLLKFKGEPMWGAHVFAAGNQSAERRSFRTSRRRL
ID DRRQQRVKLVQEIFAPVISPIDPRFFIRLHESALWRDDVAETDKHIFFNDPTYTDKEYYS
NO: DYPTIHHLIVDLMESSEKHDPRLVYLAVAWLVAHRGHFLNEVDKDNIGDVLSFDAFYPEF
1 LAFLSDNGVSPWVCESKALQATLLSRNSVNDKYKALKSLIFGSQKPEDNFDANISEDGLI
QLLAGKKVKVNKLFPQESNDASFTLNDKEDAIEEILGTLTPDECEWIAHIRRLFDWAIMK
HALKDGRTISESKVKLYEQHHHDLTQLKYFVKTYLAKEYDDIFRNVDSETTKNYVAYSYH
VKEVKGTLPKNKATQEEFCKYVLGKVKNIECSEADKVDFDEMIQRLTDNSFMPKQVSGEN
RVIPYQLYYYELKTILNKAASYLPFLTQCGKDAISNQDKLLSIMTFRIPYFVGPLRKDNS
EHAWLERKAGKIYPWNFNDKVDLDKSEEAFIRRMTNTCTYYPGEDVLPLDSLIYEKFMIL
NEINNIRIDGYPISVDVKQQVFGLFEKKRRVTVKDIQNLLLSLGALDKHGKLTGIDTTIH
SNYNTYHHFKSLMERGVLTRDDVERIVERMTYSDDTKRVRLWLNNNYGTLTADDVKHISR
LRKHDFGRLSKMFLTGLKGVHKETGERASILDFMWNTNDNLMQLLSECYTFSDEITKLQE
AYYAKAQLSLNDFLDSMYISNAVKRPIYRTLAVVNDIRKACGTAPKRIFIEMARDGESKK
KRSVTRREQIKNLYRSIRKDFQQEVDFLEKILENKSDGQLQSDALYLYFAQLGRDMYTGD
PIKLEHIKDQSFYNIDHIYPQSMVKDDSLDNKVLVQSEINGEKSSRYPLDAAIRNKMKPL
WDAYYNHGLISLKKYQRLTRSTPFTDDEKWDFINRQLVETRQSTKALAILLKRKFPDTEI
VYSKAGLSSDFRHEFGLVKSRNINDLHHAKDAFLAIVTGNVYHERFNRRWFMVNQPYSVK
TKTLFTHSIKNGNFVAWNGEEDLGRIVKMLKQNKNTIHFTRFSFDRKEGLFDIQPLKAST
GLVPRKAGLDVVKYGGYDKSTAAYYLLVRFTLEDKKTQHKLMMIPVEGLYKARIDHDKEF
LTDYAQTTISEILQKDKQKVINIMFPMGTRHIKLNSMISIDGFYLSIGGKSSKGKSVLCH
AMVPLIVPHKIECYIKAMESFARKFKENNKLRIVEKFDKITVEDNLNLYELFLQKLQHNP
YNKFFSTQFDVLTNGRSTFTKLSPEEQVQTLLNILSIFKTCRSSGCDLKSINGSAQAARI
MISADLTGLSKKYSDIRLVEQSASGLFVSKSQNLLEYL*
SEQ MSSLTKFTNKYSKQLTIKNELIPVGKTLENIKENGLIDGDEQLNENYQKAKIIVDDFLRD
ID FINKALNNTQIGNWRELADALNKEDEDNIEKLQDKIRGIIVSKFETFDLFSSYSIKKDEK
NO: IIDDDNDVEEEELDLGKKTSSFKYIFKKNLFKLVLPSYLKTTNQDKLKIISSFDNFSTYF
2 RGFFENRKNIFTKKPISTSIAYRIVHDNFPKFLDNIRCFNVWQTECPQLIVKADNYLKSK
NVIAKDKSLANYFTVGAYDYFLSQNGIDFYNNIIGGLPAFAGHEKIQGLNEFINQECQKD
SELKSKLKNRHAFKMAVLFKQILSDREKSFVIDEFESDAQVIDAVKNFYAEQCKDNNVIF
NLLNLIKNIAFLSDDELDGIFIEGKYLSSVSQKLYSDWSKLRNDIEDSANSKQGNKELAK
KIKTNKGDVEKAISKYEFSLSELNSIVHDNTKFSDLLSCTLHKVASEKLVKVNEGDWPKH
LKNNEEKQKIKEPLDALLEIYNTLLIFNCKSFNKNGNFYVDYDRCINELSSVVYLYNKTR
NYCTKKPYNTDKFKLNFNSPQLGEGFSKSKENDCLTLLFKKDDNYYVGIIRKGAKINFDD
TQAIADNTDNCIFKMNYFLLKDAKKFIPKCSIQLKEVKAHFKKSEDDYILSDKEKFASPL
VIKKSTFLLATAHVKGKKGNIKKFQKEYSKENPTEYRNSLNEWIAFCKEFLKTYKAATIF
DITTLKKAEEYADIVEFYKDVDNLCYKLEFCPIKTSFIENLIDNGDLYLFRINNKDFSSK
STGTKNLHTLYLQAIFDERNLNNPTIMLNGGAELFYRKESIEQKNRITHKAGSILVNKVC
KDGTSLDDKIRNEIYQYENKFIDTLSDEAKKVLPNVIKKEATHDITKDKRFTSDKFFFHC
PLTINYKEGDTKQFNNEVLSFLRGNPDINIIGIDRGERNLIYVTVINQKGEILDSVSFNT
VTNKSSKIEQTVDYEEKLAVREKERIEAKRSWDSISKIATLKEGYLSAIVHEICLLMIKH
NAIVVLENLNAGFKRIRGGLSEKSVYQKFEKMLINKLNYFVSKKESDWNKPSGLLNGLQL
SDQFESFEKLGIQSGFIFYVPAAYTSKIDPTTGFANVLNLSKVRNVDAIKSFFSNFNEIS
YSKKEALFKFSFDLDSLSKKGFSSFVKFSKSKWNVYTFGERIIKPKNKQGYREDKRINLT
FEMKKLLNEYKVSFDLENNLIPNLTSANLKDTFWKELFFIFKTTLQLRNSVTNGKEDVLI
SPVKNAKGEFFVSGTHNKTLPQDCDANGAYHIALKGLMILERNNLVREEKDTKKIMAISN
VDWFEYVQKRRGVL*
SEQ MNNYDEFTKLYPIQKTIRFELKPQGRTMEHLETFNFFEEDRDRAEKYKILKEAIDEYHKK
ID FIDEHLTNMSLDWNSLKQISEKYYKSREEKDKKVFLSEQKRMRQEIVSEFKKDDRFKDLF
NO: SKKLFSELLKEEIYKKGNHQEIDALKSFDKFSGYFIGLHENRKNMYSDGDEITAISNRIV
3 NENFPKFLDNLQKYQEARKKYPEWIIKAESALVAHNIKMDEVFSLEYFNKVLNQEGIQRY
NLALGGYVTKSGEKMMGLNDALNLAHQSEKSSKGRIHMTPLFKQILSEKESFSYIPDVFT
EDSQLLPSIGGFFAQIENDKDGNIFDRALELISSYAEYDTERIYIRQADINRVSNVIFGE
WGTLGGLMREYKADSINDINLERTCKKVDKWLDSKEFALSDVLEAIKRTGNNDAFNEYIS
KMRTAREKIDAARKEMKFISEKISGDEESIHIIKTLLDSVQQFLHFFNLFKARQDIPLDG
AFYAEFDEVHSKLFAIVPLYNKVRNYLTKNNLNTKKIKLNFKNPTLANGWDQNKVYDYAS
LIFLRDGNYYLGIINPKRKKNIKFEQGSGNGPFYRKMVYKQIPGPNKNLPRVFLTSTKGK
KEYKPSKEIIEGYEADKHIRGDKFDLDFCHKLIDFFKESIEKHKDWSKFNFYFSPTESYG
DISEFYLDVEKQGYRMHFENISAETIDEYVEKGDLFLFQIYNKDFVKAATGKKDMHTIYW
NAAFSPENLQDVVVKLNGEAELFYRDKSDIKEIVHREGEILVNRTYNGRTPVPDKIHKKL
TDYHNGRTKDLGEAKEYLDKVRYFKAHYDITKDRRYLNDKIYFHVPLTLNFKANGKKNLN
KMVIEKFLSDEKAHIIGIDRGERNLLYYSIIDRSGKIIDQQSLNVIDGFDYREKLNQREI
EMKDARQSWNAIGKIKDLKEGYLSKAVHEITKMAIQYNAIVVMEELNYGFKRGRFKVEKQ
IYQKFENMLIDKMNYLVFKDAPDESPGGVLNAYQLTNPLESFAKLGKQTGILFYVPAAYT
SKIDPTTGFVNLFNTSSKTNAQERKEFLQKFESISYSAKDGGIFAFAFDYRKFGTSKTDH
KNVWTAYTNGERMRYIKEKKRNELFDPSKEIKEALTSSGIKYDGGQNILPDILRSNNNGL
IYTMYSSFIAAIQMRVYDGKEDYIISPIKNSKGEFFRTDPKRRELPIDADANGAYNIALR
GELTMRAIAEKFDPDSEKMAKLELKHKDWFEFMQTRGD*
SEQ MTKTFDSEFFNLYSLQKTVRFELKPVGETASFVEDFKNEGLKRVVSEDERRAVDYQKVKE
ID IIDDYHRDFIEESLNYFPEQVSKDALEQAFHLYQKLKAAKVEEREKALKEWEALQKKLRE
NO: KVVKCFSDSNKARFSRIDKKELIKEDLINWLVAQNREDDIPTVETFNNFTTYFTGFHENR
4 KNIYSKDDHATAISFRLIHENLPKFFDNVISFNKLKEGFPELKFDKVKEDLEVDYDLKHA
FEIEYFVNFVTQAGIDQYNYLLGGKTLEDGTKKQGMNEQINLFKQQQTRDKARQIPKLIP
LFKQILSERTESQSFIPKQFESDQELFDSLQKLHNNCQDKFTVLQQAILGLAEADLKKVF
IKTSDLNALSNTIFGNYSVFSDALNLYKESLKTKKAQEAFEKLPAHSIHDLIQYLEQFNS
SLDAEKQQSTDTVLNYFIKTDELYSRFIKSTSEAFTQVQPLFELEALSSKRRPPESEDEG
AKGQEGFEQIKRIKAYLDTLMEAVHFAKPLYLVKGRKMIEGLDKDQSFYEAFEMAYQELE
SLIIPIYNKARSYLSRKPFKADKFKINFDNNTLLSGWDANKETANASILFKKDGLYYLGI
MPKGKTFLFDYFVSSEDSEKLKQRRQKTAEEALAQDGESYFEKIRYKLLPGASKMLPKVF
FSNKNIGFYNPSDDILRIRNTASHTKNGTPQKGHSKVEFNLNDCHKMIDFFKSSIQKHPE
WGSFGFTFSDTSDFEDMSAFYREVENQGYVISFDKIKETYIQSQVEQGNLYLFQIYNKDF
SPYSKGKPNLHTLYWKALFEEANLNNVVAKLNGEAEIFFRRHSIKASDKVVHPANQAIDN
KNPHTEKTQSTFEYDLVKDKRYTQDKFFFHVPISLNFKAQGVSKFNDKVNGFLKGNPDVN
IIGIDRGERHLLYFTVVNQKGEILVQESLNTLMSDKGHVNDYQQKLDKKEQERDAARKSW
TTVENIKELKEGYLSHVVHKLAHLIIKYNAIVCLEDLNFGFKRGRFKVEKQVYQKFEKAL
IDKLNYLVFKEKELGEVGHYLTAYQLTAPFESFKKLGKQSGILFYVPADYTSKIDPTTGF
VNFLDLRYQSVEKAKQLLSDFNAIRFNSVQNYFEFEIDYKKLTPKRKVGTQSKWVICTYG
DVRYQNRRNQKGHWETEEVNVTEKLKALFASDSKTTTVIDYANDDNLIDVILEQDKASFF
KELLWLLKLTMTLRHSKIKSEDDFILSPVKNEQGEFYDSRKAGEVWPKDADANGAYHIAL
KGLWNLQQINQWEKGKTLNLAIKNQDWFSFIQEKPYQE*
SEQ MHTGGLLSMDAKEFTGQYPLSKTLRFELRPIGRTWDNLEASGYLAEDRHRAECYPRAKEL
ID LDDNHRAFLNRVLPQIDMDWHPIAEAFCKVHKNPGNKELAQDYNLQLSKRRKEISAYLQD
NO: ADGYKGLFAKPALDEAMKIAKENGNESDIEVLEAFNGFSVYFTGYHESRENIYSDEDMVS
5 VAYRITEDNFPRFVSNALIFDKLNESHPDIISEVSGNLGVDDIGKYFDVSNYNNFLSQAG
IDDYNHIIGGHTTEDGLIQAFNVVLNLRHQKDPGFEKIQFKQLYKQILSVRTSKSYIPKQ
FDNSKEMVDCICDYVSKIEKSETVERALKLVRNISSFDLRGIFVNKKNLRILSNKLIGDW
DAIETALMHSSSSENDKKSVYDSAEAFTLDDIFSSVKKFSDASAEDIGNRAEDICRVISE
TAPFINDLRAVDLDSLNDDGYEAAVSKIRESLEPYMDLFHELEIFSVGDEFPKCAAFYSE
LEEVSEQLIEIIPLFNKARSFCTRKRYSTDKIKVNLKFPTLADGWDLNKERDNKAAILRK
DGKYYLAILDMKKDLSSIRTSDEDESSFEKMEYKLLPSPVKMLPKIFVKSKAAKEKYGLT
DRMLECYDKGMHKSGSAFDLGFCHELIDYYKRCIAEYPGWDVFDFKFRETSDYGSMKEFN
EDVAGAGYYMSLRKIPCSEVYRLLDEKSIYLFQIYNKDYSENAHGNKNMHTMYWEGLFSP
QNLESPVFKLSGGAELFFRKSSIPNDAKTVHPKGSVLVPRNDVNGRRIPDSIYRELTRYF
NRGDCRISDEAKSYLDKVKTKKADHDIVKDRRFTVDKMMFHVPIAMNFKAISKPNLNKKV
IDGIIDDQDLKIIGIDRGERNLIYVTMVDRKGNILYQDSLNILNGYDYRKALDVREYDNK
EARRNWTKVEGIRKMKEGYLSLAVSKLADMIIENNAIIVMEDLNHGFKAGRSKIEKQVYQ
KFESMLINKLGYMVLKDKSIDQSGGALHGYQLANHVTTLASVGKQCGVIFYIPAAFTSKI
DPTTGFADLFALSNVKNVASMREFFSKMKSVIYDKAEGKFAFTFDYLDYNVKSECGRTLW
TVYTVGERFTYSRVNREYVRKVPTDIIYDALQKAGISVEGDLRDRIAESDGDTLKSIFYA
FKYALDMRVENREEDYIQSPVKNASGEFFCSKNAGKSLPQDSDANGAYNIALKGILQLRM
LSEQYDPNAESIRLPLITNKAWLTFMQSGMKTWKN*
SEQ MDSLKDFTNLYPVSKTLRFELKPVGKTLENIEKAGILKEDEHRAESYRRVKKIIDTYHKV
ID FIDSSLENMAKMGIENEIKAMLQSFCELYKKDHRTEGEDKALDKIRAVLRGLIVGAFTGV
NO: CGRRENTVQNEKYESLFKEKLIKEILPDFVLSTEAESLPFSVEEATRSLKEFDSFTSYFA
6 GFYENRKNIYSTKPQSTAIAYRLIHENLPKFIDNILVFQKIKEPIAKELEHIRADFSAGG
YIKKDERLEDIFSLNYYIHVLSQAGIEKYNALIGKIVTEGDGEMKGLNEHINLYNQQRGR
EDRLPLFRPLYKQILSDREQLSYLPESFEKDEELLRALKEFYDHIAEDILGRTQQLMTSI
SEYDLSRIYVRNDSQLTDISKKMLGDWNAIYMARERAYDHEQAPKRITAKYERDRIKALK
GEESISLANLNSCIAFLDNVRDCRVDTYLSTLGQKEGPHGLSNLVENVFASYHEAEQLLS
FPYPEENNLIQDKDNVVLIKNLLDNISDLQRFLKPLWGMGDEPDKDERFYGEYNYIRGAL
DQVIPLYNKVRNYLTRKPYSTRKVKLNFGNSQLLSGWDRNKEKDNSCVILRKGQNFYLAI
MNNRHKRSFENKVLPEYKEGEPYFEKMDYKFLPDPNKMLPKVFLSKKGIEIYKPSPKLLE
QYGHGTHKKGDTFSMDDLHELIDFFKHSIEAHEDWKQFGFKFSDTATYENVSSFYREVED
QGYKLSFRKVSESYVYSLIDQGKLYLFQIYNKDFSPCSKGTPNLHTLYWRMLFDERNLAD
VIYKLDGKAEIFFREKSLKNDHPTHPAGKPIKKKSRQKKGEESLFEYDLVKDRHYTMDKF
QFHVPITMNFKCSAGSKVNDMVNAHIREAKDMHVIGIDRGERNLLYICVIDSRGTILDQI
SLNTINDIDYHDLLESRDKDRQQERRNWQTIEGIKELKQGYLSQAVHRIAELMVAYKAVV
ALEDLNMGFKRGRQKVESSVYQQFEKQLIDKLNYLVDKKKRPEDIGGLLRAYQFTAPFKS
FKEMGKQNGFLFYIPAWNTSNIDPTTGFVNLFHAQYENVDKAKSFFQKFDSISYNPKKDW
FEFAFDYKNFTKKAEGSRSMWILCTHGSRIKNFRNSQKNGQWDSEEFALTEAFKSLFVRY
EIDYTADLKTAIVDEKQKDFFVDLLKLFKLTVQMRNSWKEKDLDYLISPVAGADGRFFDT
REGNKSLPKDADANGAYNIALKGLWALRQIRQTSEGGKLKLAISNKEWLQFVQERSYEKD
*
SEQ MNNGTNNFQNFIGISSLQKTLRNALIPTETTQQFIVKNGIIKEDELRGENRQILKDIMDD
ID YYRGFISETLSSIDDIDWTSLFEKMEIQLKNGDNKDTLIKEQTEYRKAIHKKFANDDRFK
NO: NMFSAKLISDILPEFVIHNNNYSASEKEEKTQVIKLFSRFATSFKDYFKNRANCFSADDI
7 SSSSCHRIVNDNAEIFFSNALVYRRIVKSLSNDDINKISGDMKDSLKEMSLEEIYSYEKY
GEFITQEGISFYNDICGKVNSFMNLYCQKNKENKNLYKLQKLHKQILCIADTSYEVPYKF
ESDEEVYQSVNGFLDNISSKHIVERLRKIGDNYNGYNLDKIYIVSKFYESVSQKTYRDWE
TINTALEIHYNNILPGNGKSKADKVKKAVKNDLQKSITEINELVSNYKLCSDDNIKAETY
IHEISHILNNFEAQELKYNPEIHLVESELKASELKNVLDVIMNAFHWCSVFMTEELVDKD
NNFYAELEEIYDEIYPVISLYNLVRNYVTQKPYSTKKIKLNFGIPTLADGWSKSKEYSNN
AIILMRDNLYYLGIFNAKNKPDKKIIEGNTSENKGDYKKMIYNLLPGPNKMIPKVFLSSK
TGVETYKPSAYILEGYKQNKHIKSSKDFDITFCHDLIDYFKNCIAIHPEWKNFGFDFSDT
STYEDISGFYREVELQGYKIDWTYISEKDIDLLQEKGQLYLFQIYNKDFSKKSTGNDNLH
TMYLKNLFSEENLKDIVLKLNGEAEIFFRKSSIKNPIIHKKGSILVNRTYEAEEKDQFGN
IQIVRKNIPENIYQELYKYFNDKSDKELSDEAAKLKNVVGHHEAATNIVKDYRYTYDKYF
LHMPITINFKANKTGFINDRILQYIAKEKDLHVIGIDRGERNLIYVSVIDTCGNIVEQKS
FNIVNGYDYQIKLKQQEGARQIARKEWKEIGKIKEIKEGYLSLVIHEISKMVIKYNAIIA
MEDLSYGFKKGRFKVERQVYQKFETMLINKLNYLVFKDISITENGGLLKGYQLTYIPDKL
KNVGHQCGCIFYVPAAYTSKIDPTTGFVNIFKFKDLTVDAKREFIKKFDSIRYDSEKNLF
CFTFDYNNFITQNTVMSKSSWSVYTYGVRIKRRFVNGRFSNESDTIDITKDMEKTLEMTD
INWRDGHDLRQDIIDYEIVQHIFEIFRLTVQMRNSLSELEDRDYDRLISPVLNENNIFYD
SAKAGDALPKDADANGAYCIALKGLYEIKQITENWKEDGKFSRDKLKISNKDWFDFIQNK
RYL*
SEQ MTNKFTNQYSLSKTLRFELIPQGKTLEFIQEKGLLSQDKQRAESYQEMKKTIDKFHKYFI
ID DLALSNAKLTHLETYLELYNKSAETKKEQKFKDDLKKVQDNLRKEIVKSFSDGDAKSIFA
NO: ILDKKELITVELEKWFENNEQKDIYFDEKFKTFTTYFTGFHQNRKNMYSVEPNSTAIAYR
8 LIHENLPKFLENAKAFEKIKQVESLQVNFRELMGEFGDEGLIFVNELEEMFQINYYNDVL
SQNGITIYNSIISGFTKNDIKYKGLNEYINNYNQTKDKKDRLPKLKQLYKQILSDRISLS
FLPDAFTDGKQVLKAIFDFYKINLLSYTIEGQEESQNLLLLIRQTIENLSSFDTQKIYLK
NDTHLTTISQQVFGDFSVFSTALNYWYETKVNPKFETEYSKANEKKREILDKAKAVFTKQ
DYFSIAFLQEVLSEYILTLDHTSDIVKKHSSNCIADYFKNHFVAKKENETDKTFDFIANI
TAKYQCIQGILENADQYEDELKQDQKLIDNLKFFLDAILELLHFIKPLHLKSESITEKDT
AFYDVFENYYEALSLLTPLYNMVRNYVTQKPYSTEKIKLNFENAQLLNGWDANKEGDYLT
TILKKDGNYFLAIMDKKHNKAFQKFPEGKENYEKMVYKLLPGVNKMLPKVFFSNKNIAYF
NPSKELLENYKKETHKKGDTFNLEHCHTLIDFFKDSLNKHEDWKYFDFQFSETKSYQDLS
GFYREVEHQGYKINFKNIDSEYIDGLVNEGKLFLFQIYSKDFSPFSKGKPNMHTLYWKAL
FEEQNLQNVIYKLNGQAEIFFRKASIKPKNIILHKKKIKIAKKHFIDKKTKTSEIVPVQT
IKNLNMYYQGKISEKELTQDDLRYIDNFSIFNEKNKTIDIIKDKRFTVDKFQFHVPITMN
FKATGGSYINQTVLEYLQNNPEVKIIGLDRGERHLVYLTLIDQQGNILKQESLNTITDSK
ISTPYHKLLDNKENERDLARKNWGTVENIKELKEGYISQVVHKIATLMLEENAIVVMEDL
NFGFKRGRFKVEKQIYQKLEKMLIDKLNYLVLKDKQPQELGGLYNALQLTNKFESFQKMG
KQSGFLFYVPAWNTSKIDPTTGFVNYFYTKYENVDKAKAFFEKFEAIRFNAEKKYFEFEV
KKYSDFNPKAEGTQQAWTICTYGERIETKRQKDQNNKFVSTPINLTEKIEDFLGKNQIVY
GDGNCIKSQIASKDDKAFFETLLYWFKMTLQMRNSETRTDIDYLISPVMNDNGTFYNSRD
YEKLENPTLPKDADANGAYHIAKKGLMLLNKIDQADLTKKVDLSISNRDWLQFVQKNK*
SEQ MEQEYYLGLDMGTGSVGWAVTDSEYHVLRKHGKALWGVRLFESASTAEERRMFRTSRRRL
ID DRRNWRIEILQEIFAEEISKKDPGFFLRMKESKYYPEDKRDINGNCPELPYALFVDDDFT
NO: DKDYHKKFPTIYHLRKMLMNTEETPDIRLVYLAIHHMMKHRGHFLLSGDINEIKEFGTTF
9 SKLLENIKNEELDWNLELGKEEYAVVESILKDNMLNRSTKKTRLIKALKAKSICEKAVLN
LLAGGTVKLSDIFGLEELNETERPKISFADNGYDDYIGEVENELGEQFYIIETAKAVYDW
AVLVEILGKYTSISEAKVATYEKHKSDLQFLKKIVRKYLTKEEYKDIFVSTSDKLKNYSA
YIGMTKINGKKVDLQSKRCSKEEFYDFIKKNVLKKLEGQPEYEYLKEELERETFLPKQVN
RDNGVIPYQIHLYELKKILGNLRDKIDLIKENEDKLVQLFEFRIPYYVGPLNKIDDGKEG
KFTWAVRKSNEKIYPWNFENVVDIEASAEKFIRRMTNKCTYLMGEDVLPKDSLLYSKYMV
LNELNNVKLDGEKLSVELKQRLYTDVFCKYRKVTVKKIKNYLKCEGIISGNVEITGIDGD
FKASLTAYHDFKEILTGTELAKKDKENIITNIVLFGDDKKLLKKRLNRLYPQITPNQLKK
ICALSYTGWGRFSKKFLEEITAPDPETGEVWNIITALWESNNNLMQLLSNEYRFMEEVET
YNMGKQTKTLSYETVENMYVSPSVKRQIWQTLKIVKELEKVMKESPKRVFIEMAREKQES
KRTESRKKQLIDLYKACKNEEKDWVKELGDQEEQKLRSDKLYLYYTQKGRCMYSGEVIEL
KDLWDNTKYDIDHIYPQSKTMDDSLNNRVLVKKKYNATKSDKYPLNENIRHERKGFWKSL
LDGGFISKEKYERLIRNTELSPEELAGFIERQIVETRQSTKAVAEILKQVFPESEIVYVK
AGTVSRFRKDFELLKVREVNDLHHAKDAYLNIVVGNSYYVKFTKNASWFIKENPGRTYNL
KKMFTSGWNIERNGEVAWEVGKKGTIVTVKQIMNKNNILVTRQVHEAKGGLFDQQIMKKG
KGQIAIKETDERLASIEKYGGYNKAAGAYFMLVESKDKKGKTIRTIEFIPLYLKNKIESD
ESIALNFLEKGRGLKEPKILLKKIKIDTLFDVDGFKMWLSGRTGDRLLFKCANQLILDEK
IIVTMKKIVKFIQRRQENRELKLSDKDGIDNEVLMEIYNTFVDKLENTVYRIRLSEQAKT
LIDKQKEFERLSLEDKSSTLFEILHIFQCQSSAANLKMIGGPGKAGILVMNNNISKCNKI
SIINQSPTGIFENEIDLLK
SEQ MNKFENFTGLYPISKTLRFELIPQGKTLEYIEKSEILENDNYRAEKYEEVKDIIDGYHKW
ID FINETLHDLHINWSELKVALENNRIEKSDASKKELQRVQKIKREEIYNAFIEHEAFQYLF
NO: KENLLSDLLPIQIEQSEDLDAEKKKQAVETFNRFSTYFTGFHENRKNIYSKEGISTSVTY
10 RIVHDNFPKFLENMKVFEILRNECPEVISDTANELAPFIDGVRIEDIFLIDFFNSTFSQN
GIDYYNRILGGVTTETGEKYRGINEFTNLYRQQHPEFGKSKKATKMVVLFKQILSDRDTL
SFIPEMFGNDKQVQNSIQLFYNREISQFENEGVKTDVCTALATLTSKIAEFDTEKIYIQQ
PELPNVSQRLFGSWNELNACLFKYAELKFGTAEKVANRKKIDKWLKSDLFSFTELNKALE
FSGKDERIENYFSETGIFAQLVKTGFDEAQSILETEYTSEVHLKDQQTDIEKIKTFLDAL
QNLMHLLKSLCVSEEADRDAAFYNEFDMLYNQLKLVVPLYNKVRNYITQKLFRSDKIKIY
FENKGQFLGGWVDSQTENSDNGTQAGGYIFRKENVINEYDYYLGICSDPKLFRRTTIVSE
NDRSSFERLDYYQLKTASVYGNSYCGKHPYTEDKNELVNSIDRFVHLSGNNILIEKIAKD
KVKSNPTTNTPSGYLNFIHREAPNTYECLLQDENFVSLNQRVVSALKATLATLVRVPKAL
VYAKKDYHLFSEIINDIDELSYEKAFSYFPVSQTEFENSSNRTIKPLLLFKISNKDLSFA
ENFEKGNRQKIGKKNLHTLYFEALMKGNQDTIDIGTGMVFHRVKSLNYNEKTLKYGHHST
QLNEKFSYPIIKDKRFASDKFLFHLSTEINYKEKRKPLNNSIIEFLTNNPDINIIGLDRG
ERHLIYLTLINQKGEILRQKTFNIVGNTNYHEKLNQREKERDNARKSWATIGKIKELKEG
FLSLVIHEIAKIMVENNAIVVLEDLNFGFKRGRFKVEKQIYQKFEKMLIDKLNYLVFKDK
KANEAGGVLKGYQLAEKFESFQKMGKQSGFLFYVPAAYTSKIDPTTGFVNMLNLNYTNMK
DAQTLLSGMDKISFNADANYFEFELDYEKFKTNQTDHTNKWTICTVGEKRFTYNSATKET
TTVNVTEDLKKLLDKFEVKYSNGDNIKDEICRQTDAKFFEIILWLLKLTMQMRNSNTKTE
EDFILSPVKNSNGEFFRSNDDANGIWPADADANGAYHIALKGLYLVKECFNKNEKSLKIE
HKNWFKFAQTRFNGSLTKNG*
SEQ MENFKNLYPINKTLRFELRPYGKTLENFKKSGLLEKDAFKANSRRSMQAIIDEKFKETIE
ID ERLKYTEFSECDLGNMTSKDKKITDKAATNLKKQVILSFDDEIFNNYLKPDKNIDALFKN
NO: DPSNPVISTFKGFTTYFVNFFEIRKHIFKGESSGSMAYRIIDENLTTYLNNIEKIKKLPE
11 ELKSQLEGIDQIDKLNNYNEFITQSGITHYNEIIGGISKSENVKIQGINEGINLYCQKNK
VKLPRLTPLYKMILSDRVSNSFVLDTIENDTELIEMISDLINKTEISQDVIMSDIQNIFI
KYKQLGNLPGISYSSIVNAICSDYDNNFGDGKRKKSYENDRKKHLETNVYSINYISELLT
DTDVSSNIKMRYKELEQNYQVCKENFNATNWMNIKNIKQSEKTNLIKDLLDILKSIQRFY
DLFDIVDEDKNPSAEFYTWLSKNAEKLDFEFNSVYNKSRNYLTRKQYSDKKIKLNFDSPT
LAKGWDANKEIDNSTIIMRKFNNDRGDYDYFLGIWNKSTPANEKIIPLEDNGLFEKMQYK
LYPDPSKMLPKQFLSKIWKAKHPTTPEFDKKYKEGRHKKGPDFEKEFLHELIDCFKHGLV
NHDEKYQDVFGFNLRNTEDYNSYTEFLEDVERCNYNLSFNKIADTSNLINDGKLYVFQIW
SKDFSIDSKGTKNLNTIYFESLFSEENMIEKMFKLSGEAEIFYRPASLNYCEDIIKKGHH
HAELKDKFDYPIIKDKRYSQDKFFFHVPMVINYKSEKLNSKSLNNRTNENLGQFTHIIGI
DRGERHLIYLTVVDVSTGEIVEQKHLDEIINTDTKGVEHKTHYLNKLEEKSKTRDNERKS
WEAIETIKELKEGYISHVINEIQKLQEKYNALIVMENLNYGFKNSRIKVEKQVYQKFETA
LIKKFNYIIDKKDPETYIHGYQLTNPITTLDKIGNQSGIVLYIPAWNTSKIDPVTGFVNL
LYADDLKYKNQEQAKSFIQKIDNIYFENGEFKFDIDFSKWNNRYSISKTKWTLTSYGTRI
QTFRNPQKNNKWDSAEYDLTEEFKLILNIDGTLKSQDVETYKKFMSLFKLMLQLRNSVTG
TDIDYMISPVTDKTGTHFDSRENIKNLPADADANGAYNIARKGIMAIENIMNGISDPLKI
SNEDYLKYIQNQQE
SEQ MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDHYKELKPIIDRIYKT
ID YADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNAIHDYFIGRTDNLTDA
NO: INKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDKFTTYFSGFYENRKNVF
12 SAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFENVKKAIGIFVSTSIEEV
FSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLNLAIQKNDETAHIIASLPH
RFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLRNENVLETAEALFNELNSID
LTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGKITKSAKEKVQRSLKHEDINL
QEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLKKQEEKEILKSQLDSLLGLYHL
LDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKARNYATKKPYSVEKFKLNFQMPTL
ASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKALSFEPTEKTSEGFDKMYYDYFPD
AAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPLEITKEIYDLNNPEKEPKKFQTAYA
KKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDLSSLRPSSQYKDLGEYYAELNPLLYH
ISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGHHGKPNLHTLYWTGLFSPENLAKTSIK
LNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKDQKTPIPDTLYQELYDYVNHRLSHDLSD
EARALLPNVITKEVSHEIIKDRRFTSDKFFFHVPITLNYQAANSPSKFNQRVNAYLKEHP
ETPIIGIDRGERNLIYITVIDSTGKILEQRSLNTIQQFDYQKKLDNREKERVAARQAWSV
VGTIKDLKQGYLSQVIHEIVDLMIHYQAVVVLENLNFGFKSKRTGIAEKAVYQQFEKMLI
DKLNCLVLKDYPAEKVGGVLNPYQLTDQFTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFV
DPFVWKTIKNHESRKHFLEGFDFLHYDVKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVF
EKNETQFDAKGTPFIAGKRIVPVIENHRFTGRYRDLYPANELIALLEEKGIVFRDGSNIL
PKLLENDDSHAIDTMVALIRSVLQMRNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPM
DADANGAYHIALKGQLLLNHLKESKDLKLQNGISNQDWLAYIQELRN*
SEQ MAVKSIKVKLRLDDMPEIRAGLWKLHKEVNAGVRYYTEWLSLLRQENLYRRSPNGDGEQE
ID CDKTAEECKAELLERLRARQVENGHRGPAGSDDELLQLARQLYELLVPQAIGAKGDAQQI
NO: ARKFLSPLADKDAVGGLGIAKAGNKPRWVRMREAGEPGWEEEKEKAETRKSADRTADVLR
13 ALADFGLKPLMRVYTDSEMSSVEWKPLRKGQAVRTWDRDMFQQAIERMMSWESWNQRVGQ
EYAKLVEQKNRFEQKNFVGQEHLVHLVNQLQQDMKEASPGLESKEQTAHYVTGRALRGSD
KVFEKWGKLAPDAPFDLYDAEIKNVQRRNTRRFGSHDLFAKLAEPEYQALWREDASFLTR
YAVYNSILRKLNHAKMFATFTLPDATAHPIWTRFDKLGGNLHQYTFLFNEFGERRHAIRF
HKLLKVENGVAREVDDVTVPISMS
EQLDNLLPRDPNEPIALYFRDYGAEQHFTGEFGGAKIQCRRDQLAHMHRRRGARDVYLNV
SVRVQSQSEARGERRPPYAAVFRLVGDNHRAFVHFDKLSDYLAEHPDDGKLGSEGLLSGL
RVMSVDLGLRTSASISVFRVARKDELKPNSKGRVPFFFPIKGNDNLVAVHERSQLLKLPG
ETESKDLRAIREERQRTLRQLRTQLAYLRLLVRCGSEDVGRRERSWAKLIEQPVDAANHM
TPDWREAFENELQKLKSLHGICSDKEWMDAVYESVRRVWRHMGKQVRDWRKDVRSGERPK
IRGYAKDVVGGNSIEQIEYLERQYKFLKSWSFFGKVSGQVIRAEKGSRFAITLREHIDHA
KEDRLKKLADRIIMEALGYVYALDERGKGKWVAKYPPCQLILLEELSEYQFNNDRPPSEN
NQLMQWSHRGVFQELINQAQVHDLLVGTMYAAFSSRFDARTGAPGIRCRRVPARCTQEHN
PEPFPWWLNKFVVEHTLDACPLRADDLIPTGEGEIFVSPFSAEEGDFHQIHADLNAAQNL
QQRLWSDFDISQIRLRCDWGEVDGELVLIPRLTGKRTADSYSNKVFYTNTGVTYYERERG
KKRRKVFAQEKLSEEEAELLVEADEAREKSVVLMRDPSGIINRGNWTRQKEFWSMVNQRI
EGYLVKQIRSRVPLQDSACENTGDI*
SEQ MATRSFILKIEPNEEVKKGLWKTHEVLNHGIAYYMNILKLIRQEAIYEHHEQDPKNPKKV
ID SKAEIQAELWDFVLKMQKCNSFTHEVDKDVVFNILRELYEELVPSSVEKKGEANQLSNKF
NO: LYPLVDPNSQSGKGTASSGRKPRWYNLKIAGDPSWEEEKKKWEEDKKKDPLAKILGKLAE
14 YGLIPLFIPFTDSNEPIVKEIKWMEKSRNQSVRRLDKDMFIQALERFLSWESWNLKVKEE
YEKVEKEHKTLEERIKEDIQAFKSLEQYEKERQEQLLRDTLNTNEYRLSKRGLRGWREII
QKWLKMDENEPSEKYLEVFKDYQRKHPREAGDYSVYEFLSKKENHFIWRNHPEYPYLYAT
FCEIDKKKKDAKQQATFTLADPINHPLWVRFEERSGSNLNKYRILTEQLHTEKLKKKLTV
QLDRLIYPTESGGWEEKGKVDIVLLPSRQFYNQIFLDIEEKGKHAFTYKDESIKFPLKGT
LGGARVQFDRDHLRRYPHKVESGNVGRIYFNMTVNIEPTESPVSKSLKIHRDDFPKFVNF
KPKELTEWIKDSKGKKLKSGIESLEIGLRVMSIDLGQRQAAAASIFEVVDQKPDIEGKLF
FPIKGTELYAVHRASFNIKLPGETLVKSREVLRKAREDNLKLMNQKLNFLRNVLHFQQFE
DITEREKRVTKWISRQENSDVPLVYQDELIQIRELMYKPYKDWVAFLKQLHKRLEVEIGK
EVKHWRKSLSDGRKGLYGISLKNIDEIDRTRKFLLRWSLRPTEPGEVRRLEPGQRFAIDQ
LNHLNALKEDRLKKMANTIIMHALGYCYDVRKKKWQAKNPACQIILFEDLSNYNPYEERS
RFENSKLMKWSRREIPRQVALQGEIYGLQVGEVGAQFSSRFHAKTGSPGIRCSVVTKEKL
QDNRFFKNLQREGRLTLDKIAVLKEGDLYPDKGGEKFISLSKDRKLVTTHADINAAQNLQ
KRFWTRTHGFYKVYCKAYQVDGQTVYIPESKDQKQKIIEEFGEGYFILKDGVYEWGNAGK
LKIKKGSSKQSSSELVDSDILKDSFDLASELKGEKLMLYRDPSGNVFPSDKWMAAGVFFG
KLERILISKLTNQYSISTIEDDSSKQSM*
SEQ MPTRTINLKLVLGKNPENATLRRALFSTHRLVNQATKRIEEFLLLCRGEAYRTVDNEGKE
ID AEIPRHAVQEEALAFAKAAQRHNGCISTYEDQEILDVLRQLYERLVPSVNENNEAGDAQA
NO: ANAWVSPLMSAESEGGLSVYDKVLDPPPVWMKLKEEKAPGWEAASQIWIQSDEGQSLLNK
15 PGSPPRWIRKLRSGQPWQDDFVSDQKKKQDELTKGNAPLIKQLKEMGLLPLVNPFFRHLL
DPEGKGVSPWDRLAVRAAVAHFISWESWNHRTRAEYNSLKLRRDEFEAASDEFKDDFTLL
RQYEAKRHSTLKSIALADDSNPYRIGVRSLRAWNRVREEWIDKGATEEQRVTILSKLQTQ
LRGKFGDPDLFNWLAQDRHVHLWSPRDSVTPLVRINAVDKVLRRRKPYALMTFAHPRFHP
RWILYEAPGGSNLRQYALDCTENALHITLPLLVDDAHGTWIEKKIRVPLAPSGQIQDLTL
EKLEKKKNRLYYRSGFQQFAGLAGGAEVLFHRPYMEHDERSEESLLERPGAVWFKLTLDV
ATQAPPNWLDGKGRVRTPPEVHHFKTALSNKSKHTRTLQPGLRVLSVDLGMRTFASCSVF
ELIEGKPETGRAFPVADERSMDSPNKLWAKHERSFKLTLPGETPSRKEEEERSIARAEIY
ALKRDIQRLKSLLRLGEEDNDNRRDALLEQFFKGWGEEDVVPGQAFPRSLFQGLGAAPFR
STPELWRQHCQTYYDKAEACLAKHISDWRKRTRPRPTSREMWYKTRSYHGGKSIWMLEYL
DAVRKLLLSWSLRGRTYGAINRQDTARFGSLASRLLHHINSLKEDRIKTGADSIVQAARG
YIPL
PHGKGWEQRYEPCQLILFEDLARYRFRVDRPRRENSQLMQWNHRAIVAETTMQAELYGQI
VENTAAGFSSRFHAATGAPGVRCRFLLERDFDNDLPKPYLLRELSWMLGNTKVESEEEKL
RLLSEKIRPGSLVPWDGGEQFATLHPKRQTLCVIHADMNAAQNLQRRFFGRCGEAFRLVC
QPHGDDVLRLASTPGARLLGALQQLENGQGAFELVRDMGSTSQMNRFVMKSLGKKKIKPL
QDNNGDDELEDVLSVLPEEDDTGRITVFRDSSGIFFPCNVWIPAKQFWPAVRAMIWKVMA
SHSLG*
SEQ MTKLRHRQKKLTHDWAGSKKREVLGSNGKLQNPLLMPVKKGQVTEFRKAFSAYARATKGE
ID MTDGRKNMFTHSFEPFKTKPSLHQCELADKAYQSLHSYLPGSLAHFLLSAHALGFRIFSK
NO: SGEATAFQASSKIEAYESKLASELACVDLSIQNLTISTLFNALTTSVRGKGEETSADPLI
16 ARFYTLLTGKPLSRDTQGPERDLAEVISRKIASSFGTWKEMTANPLQSLQFFEEELHALD
ANVSLSPAFDVLIKMNDLQGDLKNRTIVFDPDAPVFEYNAEDPADIIIKLTARYAKEAVI
KNQNVGNYVKNAITTTNANGLGWLLNKGLSLLPVSTDDELLEFIGVERSHPSCHALIELI
AQLEAPELFEKNVFSDTRSEVQGMIDSAVSNHIARLSSSRNSLSMDSEELERLIKSFQIH
TPHCSLFIGAQSLSQQLESLPEALQSGVNSADILLGSTQYMLTNSLVEESIATYQRTLNR
INYLSGVAGQINGAIKRKAIDGEKIHLPAAWSELISLPFIGQPVIDVESDLAHLKNQYQT
LSNEFDTLISALQKNFDLNFNKALLNRTQHFEAMCRSTKKNALSKPEIVSYRDLLARLTS
CLYRGSLVLRRAGIEVLKKHKIFESNSELREHVHERKHFVFVSPLDRKAKKLLRLTDSRP
DLLHVIDEILQHDNLENKDRESLWLVRSGYLLAGLPDQLSSSFINLPIITQKGDRRLIDL
IQYDQINRDAFVMLVTSAFKSNLSGLQYRANKQSFVVTRTLSPYLGSKLVYVPKDKDWLV
PSQMFEGRFADILQSDYMVWKDAGRLCVIDTAKHLSNIKKSVFSSEEVLAFLRELPHRTF
IQTEVRGLGVNVDGIAFNNGDIPSLKTFSNCVQVKVSRTNTSLVQTLNRWFEGGKVSPPS
IQFERAYYKKDDQIHEDAAKRKIRFQMPATELVHASDDAGWTPSYLLGIDPGEYGMGLSL
VSINNGEVLDSGFIHINSLINFASKKSNHQTKVVPRQQYKSPYANYLEQSKDSAAGDIAH
ILDRLIYKLNALPVFEALSGNSQSAADQVWTKVLSFYTWGDNDAQNSIRKQHWFGASHWD
IKGMLRQPPTEKKPKPYIAFPGSQVSSYGNSQRCSCCGRNPIEQLREMAKDTSIKELKIR
NSEIQLFDGTIKLFNPDPSTVIERRRHNLGPSRIPVADRTFKNISPSSLEFKELITIVSR
SIRHSPEFIAKKRGIGSEYFCAYSDCNSSLNSEANAAANVAQKFQKQLFFEL*
SEQ MKRILNSLKVAALRLLFRGKGSELVKTVKYPLVSPVQGAVEELAEAIRHDNLHLFGQKEI
ID VDLMEKDEGTQVYSVVDFWLDTLRLGMFFSPSANALKITLGKFNSDQVSPFRKVLEQSPF
NO: FLAGRLKVEPAERILSVEIRKIGKRENRVENYAADVETCFIGQLSSDEKQSIQKLANDIW
17 DSKDHEEQRMLKADFFAIPLIKDPKAVTEEDPENETAGKQKPLELCVCLVPELYTRGFGS
IADFLVQRLTLLRDKMSTDTAEDCLEYVGIEEEKGNGMNSLLGTFLKNLQGDGFEQIFQF
MLGSYVGWQGKEDVLRERLDLLAEKVKRLPKPKFAGEWSGHRMFLHGQLKSWSSNFFRLF
NETRELLESIKSDIQHATMLISYVEEKGGYHPQLLSQYRKLMEQLPALRTKVLDPEIEMT
HMSEAVRSYIMIHKSVAGFLPDLLESLDRDKDREFLLSIFPRIPKIDKKTKEIVAWELPG
EPEEGYLFTANNLFRNFLENPKHVPRFMAERIPEDWTRLRSAPVWFDGMVKQWQKVVNQL
VESPGALYQFNESFLRQRLQAMLTVYKRDLQTEKFLKLLADVCRPLVDFFGLGGNDIIFK
SCQDPRKQWQTVIPLSVPADVYTACEGLAIRLRETLGFEWKNLKGHEREDFLRLHQLLGN
LLFWIRDAKLVVKLEDWMNNPCVQEYVEARKAIDLPLEIFGFEVPIFLNGYLFSELRQLE
LLLRRKSVMTSYSVKTTGSPNRLFQLVYLPLNPSDPEKKNSNNFQERLDTPTGLSRRFLD
LTLDAFAGKLLTDPVTQELKTMAGFYDHLFGFKLPCKLAAMSNHPGSSSKMVVLAKPKKG
VASNIGFEPIPDPAHPVFRVRSSWPELKYLEGLLYLPEDTPLTIELAETSVSCQSVSSVA
FDLKNLTTILGRVGEFRVTADQPFKLTPIIPEKEESFIGKTYLGLDAGERSGVGFAIVTV
DGDGYEVQRLGVHEDTQLMALQQVASKSLKEPVFQPLRKGTFRQQERIRKSLRGCYWNFY
HALMIKYRAKVVHEESVGSSGLVGQWLRAFQKDLKKADVLPKKGGKNGVDKKKRESSAQD
TLWGGAFSKKEEQQIAFEVQAAGSSQFCLKCGWWFQLGMREVNRVQESGVVLDWNRSIVT
FLIESSGEKVYGFSPQQLEKGFRPDIETFKKMVRDFMRPPMFDRKGRPAAAYERFVLGRR
HRRYRFDKVFEERFGRSALFICPRVGCGNFDHSSEQSAVVLALIGYIADKEGMSGKKLVY
VRLAELMAEWKLKKLERSRVEEQSSAQ*
SEQ MAESKQMQCRKCGASMKYEVIGLGKKSCRYMCPDCGNHTSARKIQNKKKRDKKYGSASKA
ID QSQRIAVAGALYPDKKVQTIKTYKYPADLNGEVHDSGVAEKIAQAIQEDEIGLLGPSSEY
NO: ACWIASQKQSEPYSVVDFWFDAVCAGGVFAYSGARLLSTVLQLSGEESVLRAALASSPFV
18 DDINLAQAEKFLAVSRRTGQDKLGKRIGECFAEGRLEALGIKDRMREFVQAIDVAQTAGQ
RFAAKLKIFGISQMPEAKQWNNDSGLTVCILPDYYVPEENRADQLVVLLRRLREIAYCMG
IEDEAGFEHLGIDPGALSNFSNGNPKRGFLGRLLNNDIIALANNMSAMTPYWEGRKGELI
ERLAWLKHRAEGLYLKEPHFGNSWADHRSRIFSRIAGWLSGCAGKLKIAKDQISGVRTDL
FLLKRLLDAVPQSAPSPDFIASISALDRFLEAAESSQDPAEQVRALYAFHLNAPAVRSIA
NKAVQRSDSQEWLIKELDAVDHLEFNKAFPFFSDTGKKKKKGANSNGAPSEEEYTETESI
QQPEDAEQEVNGQEGNGASKNQKKFQRIPRFFGEGSRSEYRILTEAPQYFDMFCNNMRAI
FMQLESQPRKAPRDFKCFLQNRLQKLYKQTFLNARSNKCRALLESVLISWGEFYTYGANE
KKFRLRHEASERSSDPDYVVQQALEIARRLFLFGFEWRDCSAGERVDLVEIHKKAISFLL
AITQAEVSVGSYNWLGNSTVSRYLSVAGTDTLYGTQLEEFLNATVLSQMRGLAIRLSSQE
LKDGFDVQLESSCQDNLQHLLVYRASRDLAACKRATCPAELDPKILVLPVGAFIASVMKM
IERGDEPLAGAYLRHRPHSFGWQIRVRGVAEVGMDQGTALAFQKPTESEPFKIKPFSAQY
GPVLWLNSSSYSQSQYLDGFLSQPKNWSMRVLPQAGSVRVEQRVALIWNLQAGKMRLERS
GARAFFMPVPFSFRPSGSGDEAVLAPNRYLGLFPHSGGIEYAVVDVLDSAGFKILERGTI
AVNGFSQKRGERQEEAHREKQRRGISDIGRKKPVQAEVDAANELHRKYTDVATRLGCRIV
VQWAPQPKPGTAPTAQTVYARAVRTEAPRSGNQEDHARMKSSWGYTWGTYWEKRKPEDIL
GISTQVYWTGGIGESCPAVAVALLGHIRATSTQTEWEKEEVVFGRLKKFFPS*
SEQ MEKRINKIRKKLSADNATKPVSRSGPMKTLLVRVMTDDLKKRLEKRRKKPEVMPQVISNN
ID AANNLRMLLDDYTKMKEAILQVYWQEFKDDHVGLMCKFAQPASKKIDQNKLKPEMDEKGN
NO: LTTAGFACSQCGQPLFVYKLEQVSEKGKAYTNYFGRCNVAEHEKLILLAQLKPEKDSDEA
19 VTYSLGKFGQRALDFYSIHVTKESTHPVKPLAQIAGNRYASGPVGKALSDACMGTIASFL
SKYQDIIIEHQKVVKGNQKRLESLRELAGKENLEYPSVTLPPQPHTKEGVDAYNEVIARV
RMWVNLNLWQKLKLSRDDAKPLLRLKGFPSFPVVERRENEVDWWNTINEVKKLIDAKRDM
GRVFWSGVTAEKRNTILEGYNYLPNENDHKKREGSLENPKKPAKRQFGDLLLYLEKKYAG
DWGKVFDEAWERIDKKIAGLTSHIEREEARNAEDAQSKAVLTDWLRAKASFVLERLKEMD
EKEFYACEIQLQKWYGDLRGNPFAVEAENRVVDISGFSIGSDGHSIQYRNLLAWKYLENG
KREFYLLMNYGKKGRIRFTDGTDIKKSGKWQGLLYGGGKAKVIDLTFDPDDEQLIILPLA
FGTRQGREFIWNDLLSLETGLIKLANGRVIEKTIYNKKIGRDEPALFVALTFERREVVDP
SNIKPVNLIGVDRGENIPAVIALTDPEGCPLPEFKDSSGGPTDILRIGEGYKEKQRAIQA
AKEVEQRRAGGYSRKFASKSRNLADDMVRNSARDLFYHAVTHDAVLVFENLSRGFGRQGK
RTFMTERQYTKMEDWLTAKLAYEGLTSKTYLSKTLAQYTSKTCSNCGFTITTADYDGMLV
RLKKTSDGWATTLNNKELKAEGQITYYNRYKRQTVEKELSAELDRLSEESGNNDISKWTK
GRRDEALFLLKKRFSHRPVQEQFVCLDCGHEVHADEQAALNIARSWLFLNSNSTEFKSYK
SGKQPFVGAWQAFYKRRLKEVWKPNA
SEQ MKRINKIRRRLVKDSNTKKAGKTGPMKTLLVRVMTPDLRERLENLRKKPENIPQPISNTS
ID RANLNKLLTDYTEMKKAILHVYWEEFQKDPVGLMSRVAQPAPKNIDQRKLIPVKDGNERL
NO: TSSGFACSQCCQPLYVYKLEQVNDKGKPHTNYFGRCNVSEHERLILLSPHKPEANDELVT
20 YSLGKFGQRALDFYSIHVTRESNHPVKPLEQIGGNSCASGPVGKALSDACMGAVASFLTK
YQDIILEHQKVIKKNEKRLANLKDIASANGLAFPKITLPPQPHTKEGIEAYNNVVAQIVI
WVNLNLWQKLKIGRDEAKPLQRLKGFPSFPLVERQANEVDWWDMVCNVKKLINEKKEDGK
VFWQNLAGYKRQEALLPYLSSEEDRKKGKKFARYQFGDLLLHLEKKHGEDWGKVYDEAWE
RIDKKVEGLSKHIKLEEERRSEDAQSKAALTDWLRAKASFVIEGLKEADKDEFCRCELKL
QKWYGDLRGKPFAIEAENSILDISGFSKQYNCAFIWQKDGVKKLNLYLIINYFKGGKLRF
KKIKPEAFEANRFYTVINKKSGEIVPMEVNFNFDDPNLIILPLAFGKRQGREFIWNDLLS
LETGSLKLANGRVIEKTLYNRRTRQDEPALFVALTFERREVLDSSNIKPMNLIGIDRGEN
IPAVIALTDPEGCPLSRFKDSLGNPTHILRIGESYKEKQRTIQAAKEVEQRRAGGYSRKY
ASKAKNLADDMVRNTARDLLYYAVTQDAMLIFENLSRGFGRQGKRTFMAERQYTRMEDWL
TAKLAYEGLPSKTYLSKTLAQYTSKTCSNCGFTITSADYDRVLEKLKKTATGWMTTINGK
ELKVEGQITYYNRYKRQNVVKDLSVELDRLSEESVNNDISSWTKGRSGEALSLLKKRFSH
RPVQEKFVCLNCGFETHADEQAALNIARSWLFLRSQEYKKYQTNKTTGNTDKRAFVETWQ
SFYRKKLKEVWKP
SEQ atgGGAAAAATGTATTATCTTGGTCTGGATATAGGAACAAATTCTGTTGGATATGCCGTA
ID ACCGACCCATCGTACCATTTGCTCAAATTTAAAGGCGAACCGATGTGGGGTGCCCACGTG
NO: TTTGCTGCGGGGAATCAATCAGCTGAACGGAGAAGCTTTCGTACGAGCCGCAGACGCCTT
21 GACCGCAGGCAACAGCGTGTCAAACTGGTTCAAGAAATCTTTGCTCCCGTGATTAGTCCC
ATTGATCCACGTTTTTTTATCAGACTTCATGAGAGCGCTTTATGGCGGGATGATGTGGCT
GAAACGGATAAACATATTTTCTTTAATGACCCGACCTATACGGATAAGGAATATTATTCT
GACTATCCAACCATCCATCATCTCATTGTGGACCTTATGGAAAGCAGTGAAAAGCATGAC
CCGCGGCTTGTTTATTTGGCTGTTGCCTGGCTGGTTGCTCATCGTGGTCATTTCCTCAAT
GAAGTGGATAAGGATAATATTGGGGATGTCCTGAGTTTTGACGCCTTTTATCCTGAGTTT
CTGGCATTTCTTTCCGATAATGGGGTGTCACCTTGGGTATGTGAGTCAAAAGCACTCCAA
GCGACCCTGCTTTCACGAAACTCCGTCAACGATAAGTATAAAGCCTTGAAGTCTCTGATC
TTTGGCAGCCAAAAGCCGGAGGATAATTTTGATGCCAATATCAGTGAAGATGGACTTATC
CAACTTTTAGCAGGAAAAAAGGTCAAGGTCAATAAACTTTTTCCTCAAGAAAGTAATGAT
GCTTCCTTTACACTCAATGATAAGGAAGATGCAATTGAGGAAATCTTAGGAACGCTTACA
CCGGATGAGTGTGAATGGATTGCGCATATTAGGAGGCTGTTTGATTGGGCCATCATGAAA
CATGCTCTCAAAGATGGCAGAACAATCTCCGAATCGAAAGTAAAGCTCTATGAACAGCAT
CACCATGACTTGACACAGCTCAAGTATTTTGTGAAGACCTATCTAGCAAAGGAATATGAT
GACATTTTTCGAAACGTAGATAGTGAAACAACCAAAAACTATGTCGCATATTCCTATCAT
GTAAAAGAAGTCAAGGGTACATTGCCCAAAAATAAGGCAACCCAAGAAGAATTTTGCAAG
TATGTCCTTGGAAAGGTAAAGAACATCGAATGCAGTGAAGCTGATAAGGTTGATTTTGAT
GAAATGATTCAGCGTCTTACAGACAATTCCTTTATGCCGAAACAAGTATCAGGTGAAAAC
AGGGTTATCCCTTACCAGCTTTACTATTATGAACTAAAGACTATTTTGAATAAAGCCGCT
TCTTATCTGCCTTTTTTGACCCAATGCGGAAAAGATGCCATCTCCAATCAAGATAAGCTC
CTTTCCATCATGACCTTTCGGATTCCGTATTTCGTTGGGCCCTTGCGCAAGGACAATTCA
GAGCATGCCTGGCTGGAACGAAAAGCAGGGAAAATCTATCCGTGGAATTTTAACGACAAA
GTTGACCTTGATAAAAGTGAAGAAGCGTTCATTCGGAGAATGACGAATACCTGCACTTAT
TATCCCGGTGAAGATGTTTTGCCACTTGACTCCCTTATTTATGAAAAATTCATGATCCTC
AATGAAATCAATAATATCCGAATTGATGGTTATCCTATTTCTGTAGATGTAAAACAGCAG
GTTTTTGGCCTCTTTGAAAAGAAGAGAAGAGTGACCGTAAAGGATATCCAGAATCTCCTG
CTTTCCTTGGGTGCCTTGGATAAGCATGGTAAATTGACGGGAATCGATACTACCATCCAT
AGCAATTACAATACATACCATCATTTTAAATCGCTCATGGAGCGTGGCGTTCTTACTCGT
GATGATGTGGAACGCATTGTGGAGCGTATGACCTATAGTGATGATACAAAACGCGTCCGT
CTTTGGCTGAACAATAATTATGGAACGCTCACTGCTGACGACGTAAAGCATATTTCAAGG
CTCCGAAAGCATGATTTTGGCCGGCTTTCCAAAATGTTCCTCACAGGCCTAAAGGGAGTT
CATAAGGAAACGGGGGAACGAGCTTCCATTTTGGATTTTATGTGGAATACCAATGATAAC
TTGATGCAGCTTTTATCTGAATGTTATACTTTTTCGGATGAAATTACCAAGCTGCAGGAA
GCATACTATGCCAAGGCGCAGCTTTCCCTGAATGATTTTCTGGACTCCATGTATATTTCA
AATGCTGTCAAACGTCCTATCTATCGAACTCTTGCCGTTGTAAATGACATACGCAAAGCC
TGTGGGACGGCGCCAAAACGCATTTTTATCGAAATGGCAAGAGATGGGGAAAGCAAAAAG
AAAAGGAGCGTAACAAGAAGAGAACAAATCAAGAATCTTTATAGGTCCATCCGCAAGGAT
TTTCAGCAGGAGGTAGATTTCCTTGAAAAAATCCTTGAAAACAAAAGCGATGGACAGCTG
CAAAGCGATGCGCTCTATCTATACTTTGCGCAGCTTGGAAGGGATATGTATACCGGGGAC
CCTATCAAGTTGGAGCATATCAAGGACCAGTCCTTCTATAATATTGATCATATCTATCCC
CAAAGCATGGTCAAGGACGATAGTCTTGATAACAAGGTGTTGGTTCAATCGGAAATTAAT
GGAGAGAAGAGCAGTCGATATCCTCTTGATGCTGCTATCCGTAATAAAATGAAGCCTCTT
TGGGATGCTTATTATAACCATGGCCTGATTTCCCTCAAGAAGTATCAGCGTTTGACGCGG
AGCACTCCCTTTACAGATGATGAAAAGTGGGATTTCATCAATCGGCAGCTTGTTGAGACA
AGACAATCCACGAAGGCCTTGGCAATCTTACTAAAAAGGAAGTTCCCTGATACGGAGATT
GTCTACTCCAAGGCAGGGCTTTCTTCTGATTTTCGGCATGAGTTTGGTCTCGTAAAATCG
AGGAATATCAATGACCTGCACCATGCAAAGGACGCATTTCTTGCGATTGTAACAGGAAAT
GTCTATCATGAACGCTTTAATCGCCGGTGGTTTATGGTGAACCAGCCCTATTCCGTCAAG
ACCAAGACGTTGTTTACGCATTCTATTAAAAATGGTAATTTTGTAGCTTGGAATGGAGAA
GAGGATCTTGGCCGCATTGTTAAAATGTTAAAGCAAAATAAGAACACTATTCATTTCACG
CGGTTCTCTTTTGATCGAAAGGAAGGCCTGTTTGATATTCAGCCACTAAAAGCGTCAACC
GGTCTTGTACCAAGAAAAGCCGGACTAGACGTGGTAAAATATGGTGGCTATGACAAATCG
ACAGCAGCTTATTATCTCCTTGTTCGATTTACACTAGAAGATAAAAAGACTCAACATAAA
TTGATGATGATTCCTGTAGAAGGCTTGTATAAAGCTCGAATTGACCATGATAAGGAATTC
TTAACGGACTATGCACAAACTACAATCAGTGAAATCCTACAAAAAGATAAACAAAAGGTG
ATAAATATAATGTTTCCAATGGGAACAAGGCACATTAAACTGAATTCCATGATTTCAATC
GATGGTTTTTATCTTTCCATTGGAGGAAAGTCTAGTAAGGGAAAATCGGTGTTGTGTCAT
GCTATGGTACCTCTTATTGTACCTCATAAGATAGAATGTTATATTAAGGCGATGGAGTCT
TTTGCACGTAAATTTAAAGAAAATAATAAATTAAGGATTGTGGAAAAGTTTGATAAGATT
ACGGTGGAAGATAACTTGAACCTATACGAACTATTTTTACAAAAACTTCAACATAACCCA
TATAATAAGTTCTTCTCCACACAATTTGATGTGCTGACTAATGGAAGAAGTACATTTACT
AAATTATCTCCAGAGGAACAAGTTCAAACGTTATTGAATATCTTATCAATTTTTAAAACT
TGTCGGAGCTCTGGCTGCGATTTAAAATCCATTAACGGTTCTGCTCAAGCTGCCAGAATT
ATGATCAGCGCAGATTTAACTGGACTCTCAAAAAAATATTCCGATATTCGGCTTGTTGAG
CAATCAGCATCTGGACTTTTTGTTAGTAAATCACAAAATCTTTTGGAGTATTTAtga
SEQ atgtcttcattaacaaaatttacaaataaatacagtaagcagctaaccataaaaaatgaa
ID ctcatcccagtaggaaagactctcgagaacattaaggaaaacggtctcatagatggagat
NO: gaacagctaaacgagaattatcaaaaagcaaagataatcgttgatgattttctacgagat
22 ttcataaataaagctttaaataatacccaaataggaaattggagagaattagcagatgct
ttaaataaagaagatgaagataacatagaaaagctccaagacaaaatcagaggaataatt
gtaagtaaattcgagacatttgatttgttttcttcttactcgataaagaaagacgaaaag
ataatagatgatgataatgatgttgaagaagaggagctagatctaggaaaaaaaacttcc
tcatttaaatatatttttaagaaaaacctttttaaattagtacttccttcttatttaaag
acaacaaatcaggataaactgaaaataatctcttcttttgataatttttctacctatttc
agaggattctttgagaacagaaaaaatattttcactaagaagcctatatctacgtcaatt
gcctacagaattgtccatgataactttccaaagtttctagataacatcagatgttttaat
gtgtggcaaacagaatgcccacagttaattgtaaaggctgataattatttaaaatcaaag
aacgtcatagctaaagataaatctttagcaaactattttactgtaggagcatatgattac
ttcttatcccagaatggcattgatttctacaacaacattatcggcggtctaccagcattt
gctggtcatgagaaaatccaaggacttaatgaatttataaatcaagaatgccaaaaggac
agcgaactaaaatctaaactgaaaaacagacatgctttcaaaatggctgttctatttaag
caaattctttcagatagagaaaaaagttttgttatagacgagttcgaatctgatgctcag
gtcatagatgcggttaagaacttctatgcagaacaatgtaaggataataatgttattttt
aaccttctaaatcttatcaagaatatagcgttcttatctgatgatgaattagatggaatt
tttatagaaggcaagtatttaagctctgtttcccaaaagctatattcagattggtcgaag
cttcgaaatgatattgaagatagtgcaaacagtaaacaaggaaataaagagttagcaaag
aaaattaaaacaaataaaggcgatgttgaaaaggccataagtaaatatgagttttcttta
tcagaacttaactcaattgtacatgataatacaaaattcagtgaccttctttcttgtacg
ttacataaagtggctagcgaaaaactagtgaaagttaatgaaggggactggccaaaacac
ctgaaaaataatgaagaaaaacaaaagataaaagagcctttagatgcattgttagaaatt
tataatacattgctgatattcaactgcaagtcatttaataagaacggtaatttctatgtt
gattatgacagatgcataaatgagctttctagtgttgtttatttatataacaaaacaaga
aattactgtacaaagaaaccttataacacagacaaattcaaattaaactttaacagtcct
caattaggagagggctttagtaagtcgaaagaaaatgactgtctgacattattatttaaa
aaagacgacaattactatgttggaattatcagaaaaggggcaaaaattaactttgatgat
acacaagccattgcagacaatacagataactgtatatttaagatgaattatttcctatta
aaagatgctaaaaagtttattcctaaatgttcaattcagttaaaagaagtaaaagcacat
tttaaaaaatcagaggatgattatatcctgagtgacaaagaaaaatttgcctctcccctt
gttattaagaaatcaacatttttattagcaacagcacatgtaaaaggaaagaaaggaaac
ataaaaaaattccaaaaggaatattctaaggaaaatccaacagaatatagaaattctctg
aatgaatggattgcattttgtaaagaatttctaaaaacatataaggcggcaacaatcttt
gacattacaacgttaaaaaaagctgaagaatatgctgatattgttgagttttataaggat
gtagataatctttgttataaactagagttttgccctattaaaacatctttcattgagaat
cttattgataatggggacttatatttattcagaatcaataataaagatttcagttcaaaa
tctactggtacaaagaatcttcatacgctctatcttcaggcaatctttgatgaaagaaac
ctcaataatcctactattatgttaaatggcggagcagagttattttatcgaaaagaaagc
attgaacagaaaaataggataactcataaggcaggatcaattcttgtaaacaaggtttgt
aaggatggaacaagtctagatgacaaaatcagaaacgaaatatatcaatatgaaaacaag
tttattgatacattgtctgatgaagctaaaaaagttttacctaatgtaataaaaaaagaa
gcaactcacgacataacaaaagataagcgatttacatcagataagttctttttccattgc
ccattaacaattaactataaggaaggagatacaaaacaatttaacaatgaggttttatct
ttccttagaggtaatccagacattaatatcatcggaattgacagaggagaaagaaacctt
atatacgtaactgttattaatcagaaaggcgaaatacttgacagcgtttcgtttaacaca
gtaacaaacaagtcgagcaaaattgaacaaactgttgattatgaggaaaagcttgctgtt
agggaaaaagaaagaatagaagcaaaaagatcctgggattcaatatcaaagatagcaacc
ttaaaagaaggttatctatcagctattgttcatgagatatgcctactgatgatcaaacac
aacgcaatcgttgtacttgagaatctaaatgcaggatttaagagaattagaggaggatta
tcagaaaagtctgtttatcagaaattcgagaagatgcttattaacaaactaaattacttt
gtatctaaaaaagaatcagactggaataaacctagtggacttttaaatggtttacaactt
tcagaccagttcgagtcatttgagaaattaggaattcaatctgggttcatcttctatgtt
cctgcagcatatacatctaagattgatcctacaacaggatttgcaaatgttcttaactta
tccaaggtaagaaatgttgatgcaataaagagttttttcagtaatttcaatgaaatttca
tatagcaaaaaagaagctctctttaaattctcttttgatttagattccttatcaaagaag
ggcttcagctcatttgtaaaattcagtaaatctaaatggaatgtatatacatttggagag
agaataataaaaccaaagaataagcaagggtatcgtgaagataagagaattaatttaaca
tttgaaatgaaaaaacttctgaatgaatataaagtaagttttgatcttgaaaacaactta
attccaaatctaacctctgcaaatctgaaagataccttctggaaagaactattctttatt
tttaaaacaactctgcagcttagaaacagtgtaacaaatggcaaagaagatgtactgatt
tctccagtaaagaacgctaaaggagagttctttgtatcaggaactcataacaagacatta
cctcaagactgtgatgcaaatggagcatatcatatcgccctaaaaggtctgatgattctt
gaacgtaacaatcttgttagagaagaaaaagacacaaagaagataatggcaatttctaat
gttgactggtttgagtatgttcaaaaaaggagaggtgtcctgtaa
SEQ ATGAACAACTATGATGAGTTTACCAAACTGTACCCAATACAGAAAACGATAAGGTTCGAA
ID TTGAAGCCGCAGGGAAGAACGATGGAACACCTCGAAACATTCAACTTTTTCGAAGAGGAC
NO: AGGGATAGAGCGGAGAAATATAAGATTTTAAAGGAAGCAATCGACGAGTATCATAAGAAG
23 TTTATAGACGAACATCTAACAAATATGTCTCTTGACTGGAATTCTTTAAAACAGATTTCA
GAGAAATACTATAAGAGTAGAGAGGAAAAAGACAAGAAAGTTTTTCTGTCAGAACAGAAA
CGCATGAGGCAAGAGATAGTTTCTGAGTTCAAAAAAGACGATCGGTTTAAAGATCTTTTT
TCAAAAAAATTGTTTTCTGAACTTCTCAAGGAAGAGATTTACAAAAAAGGAAACCATCAG
GAAATTGACGCATTGAAAAGTTTTGATAAATTCTCAGGCTATTTTATTGGGTTGCATGAG
AACCGAAAAAATATGTATTCTGACGGAGACGAGATCACGGCTATCTCTAACCGTATTGTA
AATGAGAATTTCCCGAAGTTCCTCGACAACCTTCAGAAATATCAGGAAGCTCGTAAAAAA
TATCCAGAGTGGATCATTAAGGCAGAATCTGCTTTAGTTGCACATAATATCAAGATGGAT
GAAGTCTTTTCCTTAGAGTATTTCAACAAAGTCCTGAATCAAGAAGGAATACAGAGATAC
AATCTCGCCCTAGGTGGCTATGTGACCAAAAGTGGTGAGAAAATGATGGGGCTTAATGAT
GCACTTAATCTTGCCCATCAAAGTGAAAAAAGCAGCAAGGGAAGGATACACATGACTCCA
CTCTTCAAACAGATTCTGAGTGAAAAAGAGTCCTTTTCTTATATACCAGATGTTTTTACA
GAAGACTCTCAACTTTTACCATCCATTGGTGGGTTCTTTGCACAAATAGAAAATGATAAG
GACGGGAATATTTTTGACAGAGCATTAGAATTGATATCTTCTTATGCAGAATACGATACA
GAAAGGATATATATCAGGCAAGCGGACATAAACAGAGTTTCTAATGTTATTTTCGGGGAG
TGGGGAACACTGGGGGGGTTAATGAGGGAATACAAAGCAGACTCTATCAACGACATCAAT
TTGGAGAGAACATGCAAGAAGGTAGACAAGTGGCTCGACTCAAAGGAGTTTGCGTTATCA
GATGTATTAGAGGCAATAAAAAGAACCGGCAATAATGATGCTTTTAATGAATATATCTCA
AAGATGCGCACTGCCAGGGAAAAGATTGACGCTGCAAGAAAGGAAATGAAATTCATTTCG
GAAAAAATATCTGGAGACGAAGAATCGATCCATATTATCAAAACCTTATTGGACTCGGTG
CAACAGTTTTTACATTTTTTCAATTTATTCAAAGCGCGTCAGGACATTCCTCTTGATGGA
GCATTCTATGCGGAGTTCGATGAAGTCCATAGCAAACTGTTTGCTATTGTTCCGTTGTAT
AATAAGGTTAGGAACTATCTTACGAAAAATAACCTTAACACGAAAAAGATAAAGCTAAAC
TTCAAGAATCCAACTCTGGCAAACGGATGGGATCAAAACAAGGTATATGACTACGCCTCC
TTAATCTTTCTCCGCGATGGTAATTATTATCTCGGAATAATAAATCCAAAAAGGAAAAAG
AATATTAAATTCGAACAAGGGTCTGGAAATGGCCCATTCTACCGGAAGATGGTGTACAAA
CAAATTCCAGGGCCGAACAAGAACTTACCAAGAGTCTTCCTCACATCTACGAAAGGCAAA
AAAGAGTACAAGCCGTCAAAGGAGATAATAGAAGGATATGAAGCGGACAAACACATAAGA
GGAGATAAATTCGATCTGGATTTCTGTCATAAGCTGATAGACTTCTTCAAGGAATCCATC
GAGAAGCACAAGGACTGGAGTAAGTTCAACTTCTATTTCTCTCCAACTGAATCATATGGA
GACATCAGCGAATTCTATCTGGATGTAGAAAAACAGGGATACCGGATGCATTTTGAGAAT
ATTTCTGCCGAGACGATTGATGAGTATGTCGAAAAGGGGGACTTATTCCTCTTCCAGATA
TACAACAAAGACTTTGTGAAAGCGGCAACCGGAAAAAAAGATATGCACACCATTTATTGG
AACGCGGCATTCTCGCCCGAGAACCTTCAGGATGTGGTAGTGAAACTGAACGGTGAAGCA
GAACTTTTCTACAGAGACAAGAGCGACATCAAGGAGATAGTTCACAGGGAGGGAGAGATA
CTGGTCAATCGTACCTACAACGGCAGGACACCTGTGCCTGACAAGATCCACAAAAAATTA
ACAGATTATCATAATGGCCGTACCAAAGATCTCGGAGAAGCAAAAGAATACCTCGATAAG
GTCAGATATTTCAAAGCGCACTACGACATCACAAAGGATCGCAGATACCTGAATGATAAA
ATATACTTCCATGTGCCTCTGACATTGAATTTCAAAGCAAACGGGAAGAAGAATCTCAAT
AAGATGGTAATTGAAAAGTTCCTCTCGGACGAAAAAGCGCATATTATTGGGATTGATCGC
GGGGAAAGGAATCTTCTTTACTATTCTATCATTGACAGGTCAGGTAAAATAATCGATCAA
CAGAGCCTCAACGTCATCGATGGATTCGATTACCGAGAGAAACTGAATCAGAGGGAGATC
GAGATGAAGGATGCCAGACAAAGCTGGAATGCTATCGGGAAGATAAAGGACCTCAAGGAA
GGGTATCTTTCAAAAGCGGTCCACGAAATTACCAAGATGGCGATACAATACAATGCCATT
GTTGTCATGGAGGAACTCAATTATGGGTTCAAACGCGGACGTTTCAAAGTTGAGAAGCAG
ATATATCAGAAATTCGAGAATATGCTGATTGACAAGATGAATTATCTGGTATTCAAGGAT
GCTCCGGATGAAAGTCCGGGAGGAGTCCTCAATGCATATCAGCTTACTAATCCGCTTGAA
AGTTTCGCTAAACTTGGGAAACAGACAGGAATTCTTTTCTATGTTCCGGCAGCCTATACT
TCGAAGATAGATCCGACGACCGGGTTTGTCAATCTTTTCAATACTTCAAGTAAAACGAAC
GCACAGGAAAGAAAAGAATTCTTGCAAAAATTCGAGTCGATCTCCTATTCCGCTAAAGAC
GGAGGAATATTCGCATTCGCGTTCGATTATCGGAAGTTCGGAACGTCAAAAACAGACCAC
AAAAATGTATGGACCGCATACACGAACGGGGAAAGGATGAGGTACATAAAAGAGAAAAAA
CGCAACGAACTGTTCGACCCCTCGAAGGAGATCAAAGAGGCTCTCACTTCATCAGGAATC
AAATATGACGGCGGACAGAACATATTGCCAGATATCCTGAGGAGCAACAATAACGGTCTG
ATCTACACAATGTATTCCTCTTTCATAGCGGCCATTCAAATGAGGGTCTATGACGGGAAA
GAAGACTATATCATCTCGCCGATAAAGAACAGCAAGGGAGAGTTCTTCAGGACCGATCCG
AAAAGAAGGGAACTTCCGATAGACGCGGATGCGAACGGCGCGTATAACATTGCTCTCAGG
GGCGAATTGACGATGCGTGCGATAGCGGAGAAGTTCGATCCGGACTCGGAAAAGATGGCG
AAGCTAGAACTGAAACATAAGGACTGGTTCGAATTCATGCAGACAAGGGGGGATTGA
SEQ ATGACAAAAACATTTGATTCAGAATTTTTTAATTTATATTCTCTTCAAAAAACAGTTCGT
ID TTTGAACTCAAGCCGGTTGGTGAAACAGCCTCGTTTGTTGAAGATTTTAAAAACGAAGGT
NO: TTGAAACGAGTTGTTTCAGAGGATGAACGGCGTGCGGTTGATTACCAAAAAGTGAAAGAA
24 ATTATTGATGACTACCACCGAGATTTTATTGAAGAATCGCTGAACTATTTTCCTGAGCAG
GTCTCAAAAGACGCTTTGGAACAAGCTTTTCACCTTTATCAAAAACTAAAAGCCGCTAAG
GTTGAAGAGCGTGAAAAAGCATTGAAAGAATGGGAAGCCCTTCAGAAAAAACTGCGCGAA
AAAGTTGTTAAATGTTTTTCAGATTCAAACAAAGCACGCTTTTCCCGCATTGATAAAAAA
GAACTGATTAAAGAAGATTTAATTAACTGGTTGGTTGCACAAAATCGCGAAGATGACATT
CCAACCGTTGAAACCTTTAACAACTTTACGACTTATTTTACGGGGTTTCATGAAAACCGA
AAAAACATTTATTCAAAAGACGATCATGCCACAGCCATTTCATTTCGACTCATTCATGAA
AACCTGCCTAAGTTTTTTGATAATGTGATCAGCTTTAATAAATTGAAGGAAGGATTTCCA
GAGCTGAAATTTGATAAGGTTAAGGAAGATTTAGAAGTTGATTATGACTTGAAACATGCC
TTTGAAATCGAATACTTTGTCAATTTTGTTACCCAAGCCGGAATTGACCAATATAACTAT
CTTTTGGGGGGTAAAACCTTAGAAGACGGCACCAAAAAGCAAGGCATGAATGAACAAATC
AATCTGTTCAAGCAACAGCAAACCCGAGACAAAGCCCGACAAATTCCCAAACTCATACCA
TTGTTTAAACAAATTCTAAGCGAACGAACGGAAAGCCAATCGTTTATTCCAAAACAATTT
GAATCAGACCAAGAGCTATTTGACTCACTGCAAAAACTGCATAACAACTGCCAAGATAAA
TTTACCGTACTGCAACAAGCCATTTTAGGCTTAGCCGAAGCAGATCTGAAAAAAGTATTC
ATTAAAACATCTGATCTTAATGCGCTATCAAATACCATTTTTGGAAATTACAGTGTGTTT
TCGGATGCGTTGAATTTATACAAAGAATCGCTCAAAACAAAAAAGGCGCAAGAAGCGTTT
GAAAAACTACCCGCTCACAGCATTCATGACTTGATTCAATATTTGGAGCAATTTAATAGC
TCTTTGGATGCAGAAAAACAGCAATCAACTGACACCGTACTGAATTACTTTATTAAAACA
GACGAGCTGTATTCTCGGTTCATAAAATCAACGAGCGAAGCCTTCACACAAGTACAACCA
CTCTTTGAATTGGAAGCATTAAGCTCAAAACGTCGTCCACCGGAAAGTGAAGACGAAGGC
GCAAAAGGTCAGGAAGGGTTTGAGCAAATTAAACGCATAAAAGCCTATTTGGATACCTTG
ATGGAGGCGGTGCATTTTGCAAAACCACTTTATCTGGTGAAGGGGCGCAAAATGATTGAA
GGTCTGGACAAAGACCAAAGTTTCTATGAAGCCTTTGAAATGGCTTACCAAGAACTAGAA
AGTCTGATTATTCCAATCTACAACAAAGCTCGTAGTTATTTAAGTCGTAAACCGTTTAAA
GCGGACAAATTCAAAATTAATTTTGATAATAATACATTGCTTTCCGGTTGGGATGCTAAT
AAAGAAACGGCTAACGCTTCAATTTTGTTTAAGAAGGATGGTTTGTATTATTTAGGAATC
ATGCCTAAAGGAAAAACGTTTTTGTTCGATTACTTCGTTTCATCGGAAGATTCTGAAAAG
TTAAAACAAAGAAGACAAAAAACCGCCGAAGAAGCGCTTGCGCAAGATGGCGAAAGCTAC
TTTGAAAAAATTCGTTACAAGCTGTTACCTGGCGCCAGCAAAATGTTGCCGAAAGTATTT
TTTTCCAACAAAAACATAGGGTTTTACAACCCAAGTGATGACATACTTCGTATCAGGAAT
ACAGCCTCTCACACTAAAAACGGAACACCGCAAAAAGGGCACTCTAAAGTAGAGTTTAAT
TTGAATGATTGTCATAAGATGATTGATTTCTTTAAATCAAGCATTCAAAAGCATCCAGAG
TGGGGAAGTTTTGGATTCACCTTTTCAGATACATCAGATTTTGAAGATATGAGCGCCTTT
TATCGAGAAGTCGAAAACCAAGGTTATGTCATTAGTTTCGATAAAATAAAAGAAACTTAC
ATTCAGAGTCAAGTTGAACAGGGGAACCTATATTTATTCCAAATCTACAATAAAGACTTC
TCGCCCTACAGCAAAGGCAAACCAAATTTACACACGCTTTACTGGAAAGCGTTGTTTGAG
GAAGCCAACCTAAATAATGTGGTGGCAAAACTCAATGGTGAAGCTGAAATTTTCTTTAGG
CGACACTCAATCAAAGCATCTGATAAAGTGGTGCACCCAGCGAATCAAGCCATTGACAAT
AAAAACCCGCATACCGAAAAAACGCAAAGCACCTTTGAATATGATCTTGTAAAAGACAAG
CGCTATACCCAAGACAAATTCTTCTTCCATGTACCGATTTCATTGAACTTTAAGGCACAA
GGTGTTTCAAAATTTAACGATAAAGTGAATGGATTTTTAAAGGGTAACCCAGATGTCAAT
ATTATTGGCATTGACCGAGGCGAACGACACCTTCTGTATTTCACTGTGGTGAATCAGAAA
GGTGAAATTTTGGTTCAAGAGTCGCTTAATACCCTAATGAGTGATAAAGGGCATGTGAAT
GACTACCAGCAAAAACTCGACAAAAAAGAACAAGAACGCGATGCCGCTCGCAAAAGCTGG
ACGACGGTTGAAAATATCAAAGAATTAAAAGAAGGCTATTTATCTCATGTTGTTCATAAG
TTGGCACACCTGATTATTAAATACAATGCCATTGTTTGCTTGGAAGACCTGAATTTTGGT
TTCAAACGCGGGCGTTTTAAAGTGGAAAAACAAGTTTATCAGAAATTTGAAAAAGCGCTT
ATTGATAAGCTTAACTACTTGGTATTTAAAGAAAAAGAGTTAGGCGAGGTGGGCCATTAT
CTAACCGCCTATCAGTTGACCGCACCGTTTGAAAGTTTCAAGAAGTTAGGCAAGCAAAGT
GGCATATTGTTTTATGTTCCGGCGGATTACACCTCCAAAATTGACCCAACCACCGGGTTT
GTCAACTTTCTTGATCTGCGTTATCAGAGTGTCGAAAAAGCCAAACAGCTCTTAAGCGAC
TTTAATGCCATTCGTTTTAATTCAGTACAAAACTATTTTGAGTTCGAAATAGATTACAAA
AAACTCACACCCAAACGTAAAGTTGGTACTCAGAGTAAATGGGTGATTTGTACCTATGGA
GATGTCCGCTATCAAAATCGGCGTAATCAAAAAGGTCACTGGGAAACGGAAGAAGTCAAT
GTGACTGAAAAACTAAAAGCCCTTTTCGCCAGTGATTCCAAAACTACAACCGTAATCGAT
TACGCCAATGACGACAACCTAATTGACGTCATTCTGGAACAGGACAAAGCCAGCTTCTTC
AAAGAACTGTTATGGTTATTAAAACTCACCATGACGCTCCGCCACAGCAAAATCAAAAGT
GAAGACGACTTTATTCTTTCACCCGTTAAAAACGAACAAGGCGAGTTTTACGATAGTCGA
AAAGCGGGCGAGGTGTGGCCTAAAGATGCAGACGCCAATGGCGCTTATCACATAGCGTTG
AAAGGCTTGTGGAATCTGCAACAGATCAATCAGTGGGAAAAGGGTAAAACACTTAATCTG
GCGATTAAAAACCAGGATTGGTTCAGTTTTATTCAAGAAAAGCCCTATCAAGAATAA
SEQ ATGCACACAGGCGGATTACTTAGCATGGATGCCAAGGAGTTTACCGGACAGTACCCCCTT
ID TCGAAGACTCTGCGTTTTGAACTGAGACCGATAGGCAGAACGTGGGACAATCTCGAAGCA
NO: TCGGGGTATCTTGCGGAGGACAGACACCGTGCAGAATGCTATCCCAGGGCAAAAGAGCTC
25 TTGGACGACAACCATCGTGCATTCCTCAACCGTGTCCTGCCTCAGATCGATATGGATTGG
CACCCGATCGCAGAGGCATTCTGCAAAGTCCACAAGAATCCGGGAAACAAGGAATTGGCT
CAGGATTACAATCTTCAGCTGTCCAAACGCAGAAAGGAGATTTCGGCCTATCTGCAGGAT
GCGGACGGCTATAAAGGTCTGTTTGCCAAACCTGCATTGGATGAAGCAATGAAGATCGCG
AAAGAAAACGGAAATGAATCGGACATAGAGGTTCTTGAGGCATTCAACGGTTTCTCCGTA
TACTTCACCGGATATCATGAGAGCAGGGAGAACATCTATTCGGACGAGGATATGGTGTCG
GTAGCTTATCGCATCACCGAAGACAATTTCCCGAGATTCGTTTCCAATGCGCTTATATTC
GATAAGCTGAATGAGTCGCACCCCGATATAATCTCGGAAGTATCCGGAAATCTGGGCGTA
GACGACATCGGAAAATATTTTGATGTGTCTAACTACAATAATTTCCTGTCGCAGGCCGGT
ATAGATGACTACAATCACATCATCGGCGGCCATACGACGGAGGACGGTCTGATCCAGGCA
TTCAATGTTGTTCTGAATCTCAGGCATCAGAAAGACCCCGGATTCGAAAAAATCCAATTC
AAACAGCTGTACAAACAGATACTCAGCGTCCGTACATCCAAATCCTATATCCCGAAACAG
TTCGATAATTCGAAGGAGATGGTGGACTGCATCTGCGACTATGTGTCCAAGATCGAAAAA
TCCGAAACGGTCGAGAGAGCATTGAAGCTGGTAAGGAACATATCTTCTTTTGATTTGCGC
GGAATATTCGTAAACAAGAAGAATCTCCGCATTCTTTCCAACAAACTGATTGGTGATTGG
GACGCGATCGAAACCGCGCTGATGCACTCCTCCTCTTCGGAAAATGATAAGAAATCCGTC
TACGACAGCGCCGAGGCATTTACGCTGGATGATATCTTTTCGTCCGTTAAAAAATTCTCA
GATGCATCTGCAGAGGATATCGGAAACCGGGCGGAGGACATATGCAGAGTCATATCTGAG
ACCGCTCCGTTCATAAACGATCTGAGGGCTGTCGATTTGGACAGTTTGAATGACGACGGT
TACGAGGCGGCGGTTTCCAAGATAAGGGAATCTCTGGAACCATATATGGATCTGTTTCAT
GAACTGGAGATATTCTCCGTAGGCGATGAATTCCCGAAATGTGCAGCTTTCTACAGTGAA
CTTGAAGAAGTCTCCGAACAGCTAATCGAGATTATACCGTTATTCAACAAGGCCCGTTCG
TTCTGTACGCGCAAGAGATACAGTACGGACAAGATAAAGGTCAATTTGAAATTCCCGACA
CTCGCCGACGGATGGGATCTCAACAAAGAACGCGACAACAAAGCCGCAATACTCAGGAAA
GACGGAAAGTACTACCTGGCCATACTGGATATGAAGAAAGATCTTTCTTCGATCAGAACT
TCGGATGAAGACGAATCCAGTTTTGAGAAAATGGAGTACAAGCTTCTTCCGAGTCCGGTA
AAGATGCTGCCAAAGATCTTCGTAAAATCGAAGGCGGCCAAGGAGAAGTACGGTCTGACC
GACCGTATGCTGGAGTGCTACGATAAAGGGATGCACAAGAGCGGCAGTGCATTCGATCTC
GGATTTTGTCACGAATTGATCGATTACTACAAGAGGTGCATCGCAGAATATCCCGGCTGG
GACGTCTTCGATTTCAAGTTCAGGGAAACATCGGATTATGGCAGCATGAAGGAGTTCAAT
GAGGATGTTGCAGGGGCCGGATACTATATGTCCCTCAGAAAGATCCCTTGTTCGGAGGTC
TACAGGCTTCTTGATGAGAAATCGATATATCTTTTCCAGATCTACAACAAAGATTATTCG
GAAAACGCTCATGGGAATAAGAACATGCATACCATGTATTGGGAAGGGCTCTTTTCCCCC
CAGAATCTGGAATCCCCTGTGTTTAAACTCAGCGGCGGTGCGGAGCTTTTCTTCCGTAAA
TCCTCCATACCCAATGACGCCAAAACGGTCCATCCGAAGGGAAGCGTCCTGGTTCCGCGC
AATGATGTAAACGGCCGCAGGATACCTGACAGCATATATCGGGAGCTCACCAGATATTTC
AACCGCGGAGATTGCCGCATAAGCGACGAGGCAAAGAGTTATCTGGACAAGGTGAAAACC
AAGAAAGCTGACCACGATATCGTGAAAGACAGGAGGTTCACGGTGGACAAGATGATGTTC
CACGTCCCTATCGCCATGAATTTCAAAGCGATTTCGAAGCCGAATCTCAATAAAAAGGTG
ATTGACGGCATAATCGACGACCAAGATCTGAAGATCATCGGCATAGACCGCGGAGAGCGC
AACCTCATCTACGTAACCATGGTGGATCGCAAAGGGAACATCCTCTATCAGGATAGCCTC
AATATTCTGAACGGATACGATTACCGTAAGGCCCTCGACGTCCGCGAATATGACAATAAA
GAGGCTCGGAGGAACTGGACGAAGGTCGAAGGCATCCGTAAGATGAAAGAGGGGTATCTG
TCGCTTGCAGTCAGCAAATTGGCAGATATGATCATAGAGAACAATGCGATTATCGTCATG
GAGGATCTCAATCACGGATTCAAGGCAGGGCGTTCGAAGATAGAGAAACAGGTCTATCAG
AAGTTCGAATCCATGCTCATAAACAAACTCGGTTACATGGTCCTCAAGGATAAGTCTATC
GATCAGAGCGGCGGAGCTCTCCACGGATACCAGCTTGCCAACCATGTGACAACATTGGCA
TCTGTAGGTAAACAATGTGGAGTGATATTCTACATCCCTGCTGCATTTACATCCAAGATA
GATCCGACAACAGGATTTGCAGATCTGTTCGCCCTCAGCAATGTTAAAAACGTGGCATCT
ATGAGAGAATTTTTCTCCAAGATGAAGTCTGTAATCTATGATAAGGCGGAGGGAAAATTC
GCATTTACCTTCGACTATCTTGATTATAATGTGAAATCCGAGTGCGGAAGGACCCTTTGG
ACCGTGTATACGGTCGGAGAGAGATTCACATACAGCAGGGTCAATAGAGAATATGTCAGA
AAAGTTCCGACAGACATAATCTACGACGCATTGCAAAAGGCAGGAATATCTGTTGAAGGG
GATCTCAGGGACAGGATTGCTGAATCGGATGGCGACACTCTGAAGAGCATATTCTATGCA
TTCAAGTATGCATTGGATATGAGAGTAGAGAACCGCGAAGAGGATTACATACAGTCTCCT
GTCAAAAATGCCTCCGGAGAATTCTTCTGTTCCAAGAACGCAGGCAAATCGCTCCCTCAG
GATTCCGATGCGAACGGTGCATACAATATCGCACTCAAGGGGATCCTGCAGCTACGTATG
CTTTCCGAGCAGTATGATCCGAATGCAGAGAGCATACGGTTGCCACTGATAACCAACAAG
GCCTGGCTGACCTTTATGCAGTCCGGTATGAAGACATGGAAGAACTGA
SEQ atgGATAGTTTGAAAGATTTCACCAATCTGTACCCTGTCAGTAAGACATTGAGATTTGAA
ID TTAAAGCCCGTTGGAAAGACTTTAGAAAATATCGAGAAAGCAGGTATTTTGAAAGAGGAT
NO: GAGCATCGTGCAGAAAGTTATCGGAGGGTGAAGAAAATAATTGATACTTATCATAAGGTA
26 TTTATCGATTCTTCTCTTGAAAATATGGCTAAAATGGGTATTGAGAATGAAATAAAAGCA
ATGCTCCAAAGTTTCTGCGAATTGTATAAAAAAGATCATCGCACTGAGGGTGAAGACAAG
GCATTAGATAAAATTCGAGCAGTACTTCGTGGCCTGATTGTTGGGGCTTTCACTGGTGTT
TGCGGAAGACGGGAAAATACAGTCCAAAACGAGAAGTACGAGAGTTTGTTCAAAGAAAAG
TTGATAAAAGAAATTTTACCTGATTTTGTGCTCTCTACTGAGGCTGAAAGCTTGCCTTTC
TCTGTTGAAGAAGCTACGAGGTCACTGAAGGAGTTTGATAGCTTTACATCCTACTTTGCT
GGTTTTTACGAGAATAGAAAGAATATATACTCGACGAAACCTCAATCCACTGCCATTGCT
TATCGTCTTATTCATGAGAACTTGCCGAAGTTCATTGATAATATTCTTGTTTTTCAGAAG
ATCAAAGAGCCTATAGCCAAAGAGCTGGAACATATTCGTGCGGACTTTTCTGCCGGGGGG
TACATAAAAAAGGATGAGAGATTGGAGGATATTTTTTCGTTGAACTATTATATCCACGTG
TTATCTCAGGCTGGGATCGAAAAATATAACGCATTGATTGGGAAGATTGTGACAGAAGGA
GATGGAGAGATGAAAGGGCTCAATGAACACATCAACCTTTACAACCAACAAAGAGGCAGA
GAGGATCGGCTCCCTCTTTTTAGGCCTCTTTATAAACAGATATTGAGTGACAGAGAGCAA
TTATCATACTTGCCTGAGAGTTTTGAAAAAGATGAGGAGCTCCTCAGGGCTCTAAAAGAG
TTCTATGATCATATCGCAGAAGACATTCTCGGACGTACTCAACAGTTGATGACTTCTATT
TCAGAATATGATTTATCTCGGATATACGTAAGGAACGATAGCCAATTGACTGATATATCA
AAAAAAATGTTGGGAGATTGGAATGCTATCTACATGGCTAGAGAACGAGCATATGACCAC
GAGCAGGCTCCCAAAAGAATCACGGCGAAATACGAGAGGGACAGGATTAAAGCTCTTAAA
GGAGAAGAGAGTATAAGTCTGGCAAATCTTAATAGTTGTATTGCCTTTCTGGACAATGTT
AGAGATTGCCGTGTAGATACTTATCTTTCCACACTGGGCCAGAAGGAAGGACCACATGGT
CTATCTAATCTCGTTGAGAACGTTTTTGCCTCATACCATGAAGCAGAGCAATTGTTGAGC
TTTCCATACCCCGAAGAGAATAATCTGATTCAGGACAAGGACAATGTGGTGTTAATTAAG
AATCTTCTCGACAATATCAGTGATCTGCAGAGGTTCTTGAAACCTCTTTGGGGTATGGGA
GACGAACCCGATAAAGATGAAAGATTTTATGGAGAGTATAATTATATCCGAGGAGCTCTA
GATCAGGTGATCCCTCTGTACAATAAGGTAAGGAACTACCTCACTCGGAAGCCTTATTCG
ACCAGAAAAGTAAAACTCAATTTTGGGAATTCTCAATTGCTTAGTGGTTGGGATAGAAAT
AAGGAAAAGGATAATAGCTGTGTGATTTTGCGTAAGGGGCAGAACTTCTATTTGGCTATT
ATGAACAATAGGCACAAAAGAAGTTTCGAAAACAAGGTGTTGCCCGAGTATAAGGAGGGA
GAACCTTACTTCGAAAAGATGGATTATAAATTTTTGCCTGATCCTAATAAAATGCTTCCT
AAGGTTTTTCTTTCGAAAAAAGGAATAGAGATATACAAACCAAGTCCGAAGCTTTTAGAA
CAATATGGACATGGAACTCACAAAAAGGGAGATACCTTTAGTATGGATGATTTGCACGAA
CTGATCGATTTCTTCAAACACTCAATCGAGGCTCATGAAGATTGGAAGCAATTCGGATTC
AAATTTTCTGATACGGCTACTTATGAGAATGTATCTAGTTTCTATAGAGAAGTTGAGGAT
CAGGGGTATAAGCTCTCTTTCCGAAAAGTTTCGGAATCTTATGTCTATTCATTAATAGAT
CAAGGCAAGTTGTATTTATTTCAGATATACAACAAGGACTTTTCTCCCTGCAGCAAAGGG
ACACCTAATCTGCATACCTTGTATTGGAGAATGCTTTTTGACGAGCGCAATTTGGCAGAT
GTCATATACAAACTGGATGGGAAGGCTGAAATCTTTTTCCGAGAGAAGAGTTTGAAAAAT
GATCATCCCACGCATCCGGCTGGTAAGCCTATCAAAAAGAAAAGTCGACAAAAAAAAGGA
GAGGAGAGTCTGTTTGAGTATGATTTAGTCAAGGATAGGCACTATACGATGGATAAGTTC
CAGTTTCATGTGCCTATTACTATGAATTTTAAATGTTCTGCAGGAAGCAAAGTCAATGAT
ATGGTTAATGCTCATATTCGAGAGGCAAAGGATATGCATGTCATTGGAATTGATCGTGGA
GAACGCAATCTGCTGTATATATGCGTGATAGATAGTCGAGGGACGATTTTGGATCAAATT
TCTCTGAATACGATTAACGATATAGACTATCATGATTTATTGGAGAGTCGAGACAAAGAC
CGTCAGCAGGAGCGCCGAAACTGGCAAACTATCGAAGGGATCAAGGAGCTAAAACAAGGC
TACCTTAGTCAGGCGGTTCATCGGATAGCCGAACTGATGGTGGCTTATAAGGCTGTAGTT
GCTTTGGAGGATTTGAATATGGGGTTCAAACGTGGGCGGCAGAAAGTAGAAAGTTCTGTT
TATCAGCAGTTTGAGAAACAGCTGATAGATAAGCTCAACTATCTTGTGGACAAGAAGAAA
AGGCCTGAAGATATTGGAGGATTGTTGAGAGCCTATCAATTTACGGCCCCATTTAAGAGT
TTTAAGGAAATGGGAAAGCAAAACGGCTTCTTGTTTTATATCCCGGCTTGGAACACGAGC
AACATAGATCCGACTACTGGATTTGTTAATTTATTTCATGCCCAGTATGAAAATGTAGAT
AAAGCGAAGAGCTTCTTTCAAAAGTTTGATTCAATTAGTTACAACCCGAAGAAAGACTGG
TTTGAGTTTGCATTCGATTATAAAAACTTTACTAAAAAGGCTGAAGGAAGTCGTTCTATG
TGGATATTATGCACACATGGTTCCCGAATAAAGAATTTTAGAAATTCCCAGAAGAATGGT
CAATGGGATTCCGAAGAATTCGCCTTGACGGAGGCTTTTAAGTCTCTTTTTGTGCGATAT
GAGATAGATTATACCGCTGATTTGAAAACAGCTATTGTGGACGAAAAGCAAAAAGACTTC
TTCGTGGATCTTCTGAAGCTATTCAAATTGACAGTACAGATGCGCAACAGCTGGAAAGAG
AAGGATTTGGATTATCTAATCTCTCCTGTAGCAGGGGCTGATGGCCGTTTCTTCGATACA
AGAGAGGGAAATAAAAGTCTGCCTAAGGATGCAGATGCCAATGGAGCTTATAATATTGCC
CTAAAAGGACTTTGGGCTCTACGCCAGATTCGGCAAACTTCAGAAGGCGGTAAACTCAAA
TTGGCGATTTCCAATAAGGAATGGCTACAGTTTGTGCAAGAGAGATCTTACGAGAAAGAC
tga
SEQ atgaataatggaacaaataactttcagaattttatcggaatttcttctttgcagaagact
ID cttaggaatgctctcattccaaccgaaacaacacagcaatttattgttaaaaacggaata
NO: attaaagaagatgagctaagaggagaaaatcgtcagatacttaaagatatcatggatgat
27 tattacagaggtttcatttcagaaactttatcgtcaattgatgatattgactggacttct
ttatttgagaaaatggaaattcagttaaaaaatggagataacaaagacactcttataaaa
gaacagactgaataccgtaaggcaattcataaaaaatttgcaaatgatgatagatttaaa
aatatgttcagtgcaaaattaatctcagatattcttcctgaatttgtcattcataacaat
aattattctgcatcagaaaaggaagaaaaaacacaggtaattaaattattttccagattt
gcaacgtcattcaaggactattttaaaaacagggctaattgtttttcggctgatgatata
tcttcatcttcttgtcatagaatagttaatgataatgcagagatattttttagtaatgca
ttggtgtataggagaattgtaaaaagtctttcaaatgatgatataaataaaatatccgga
gatatgaaggattcattaaaggaaatgtctctggaagaaatttattcttatgaaaaatat
ggggaatttattacacaggaaggtatatctttttataatgatatatgtggtaaagtaaat
tcatttatgaatttatattgccagaaaaataaagaaaacaaaaatctctataagctgcaa
aagcttcataaacagatactgtgcatagcagatacttcttatgaggtgccgtataaattt
gaatcagatgaagaggtttatcaatcagtgaatggatttttggacaatattagttcgaaa
catatcgttgaaagattgcgtaagattggagacaactataacggctacaatcttgataag
atttatattgttagtaaattctatgaatcagtttcacaaaagacatatagagattgggaa
acaataaatactgcattagaaattcattacaacaatatattacccggaaatggtaaatct
aaagctgacaaggtaaaaaaagcggtaaagaatgatctgcaaaaaagcattactgaaatc
aatgagcttgttagcaattataaattatgttcggatgataatattaaagctgagacatat
atacatgaaatatcacatattttgaataattttgaagcacaggagcttaagtataatcct
gaaattcatctggtggaaagtgaattgaaagcatctgaattaaaaaatgttctcgatgta
ataatgaatgcttttcattggtgttcggttttcatgacagaggagctggtagataaagat
aataatttttatgccgagttagaagagatatatgacgaaatatatccggtaatttcattg
tataatcttgtgcgtaattatgtaacgcagaagccatatagtacaaaaaaaattaaattg
aattttggtattcctacactagcggatggatggagtaaaagtaaagaatatagtaataat
gcaattattctcatgcgtgataatttgtactatttaggaatatttaatgcaaaaaataag
cctgacaaaaagataattgaaggtaatacatcagaaaataaaggggattataagaagatg
atttataatcttctgccaggaccaaataaaatgatccccaaggtattcctctcttcaaaa
accggagtggaaacatataagccgtctgcctatatattggagggctataaacaaaacaag
catattaaatcctctaaggattttgatataacattttgtcacgatttgattgattatttt
aagaactgtatagcaatacatcctgaatggaagaattttggctttgatttttctgacacc
tccacatatgaagatatcagcggattttacagagaagtcgaattacaaggttataaaatc
gactggacatatatcagcgaaaaggatattgatttgttgcaggaaaaaggacagttatat
ttattccaaatatataacaaagatttttccaagaaaagtaccggaaatgataatcttcat
actatgtatttgaagaatttgtttagtgaagagaatttaaaggatattgtactgaaatta
aacggtgaggcggaaatcttctttagaaaatcaagcataaagaatccaataattcataaa
aaaggctctattcttgttaatagaacatatgaagcagaggaaaaagatcaatttggaaat
atccagatagtcagaaaaaacataccggaaaatatatatcaggagctttataaatatttc
aatgataaaagtgataaagaactttcggatgaagcagctaagcttaagaatgtagtaggt
catcatgaggctgctacaaacatagtaaaagattatagatatacatatgataaatatttt
cttcatatgcctattacaatcaattttaaagccaataagacaggctttattaatgacaga
atattacaatatattgctaaagaaaaggatttgcatgtaataggcattgatcgtggtgaa
agaaacctgatatatgtttcagtaattgatacttgtggaaatattgttgaacaaaaatcg
tttaacattgttaatggatatgattatcagattaagctcaagcagcaggagggggcgcga
caaatcgcacgaaaagaatggaaagaaatcggcaaaataaaagaaattaaagaaggctat
ttatctcttgtaattcatgaaatttcaaagatggttattaaatataatgccataattgca
atggaggatttaagctacggatttaaaaaaggtcgtttcaaggttgagcgacaggtttac
cagaagtttgagacaatgcttatcaacaaactcaactatctggtatttaaagatatatcc
ataacggaaaacggtggtcttctaaagggataccagcttacatatattccagataaactg
aaaaatgtgggtcatcaatgtggctgtatattttatgtacctgctgcctatacatcaaaa
atagatcctacaaccggatttgtaaatatattcaaatttaaagatttaacagttgatgcg
aagagagaatttataaaaaaatttgacagtatcagatatgattcagaaaaaaatctgttt
tgttttacattcgattataataactttattacgcaaaatactgttatgtcaaagtcaagc
tggagtgtatatacgtacggagttaggataaaaagaagatttgtcaatggcaggttctca
aatgaatcggatacaattgatataacaaaagatatggaaaaaacactcgaaatgacagat
ataaattggagagatggtcatgatctgaggcaggatattattgattatgaaatcgtacaa
cacatatttgagatttttagattgactgtacaaatgagaaacagtttaagtgaattagaa
gacagggattatgaccgtttgatttctccggtgctcaatgaaaataatatattttatgat
tcagctaaagcaggagatgcgttacctaaagacgcagatgctaatggtgcatattgtata
gctctaaaaggcttgtatgaaatcaaacaaattacagagaattggaaagaagacggtaag
ttttcaagagataaacttaaaatttccaataaggactggtttgactttattcaaaataaa
aggtatttataa
SEQ atgacaaacaaatttacaaaccagtactcgctttccaaaacacttcgatttgagttgatt
ID ccacaaggaaaaacattggaatttattcaagaaaaaggattgctctctcaagataaacaa
NO: cgagcggagagttatcaagaaatgaaaaaaactattgataaatttcataaatactttatc
28 gatttagctttaagcaatgctaaactaactcatttagaaacttacttggaattatacaat
aaaagtgctgaaacaaaaaaagaacaaaaatttaaagacgatttaaagaaagtacaagac
aatttacgaaaagaaatcgttaaatctttttcagatggtgatgcaaaatcaatttttgca
attttggataaaaaagaactgattaccgtagaacttgaaaaatggtttgaaaacaacgaa
caaaaagacatttattttgacgaaaaattcaaaacgtttactacttattttactggtttt
catcaaaacagaaaaaacatgtattcggttgaacccaattctacagcaattgcttatcga
ttgattcatgaaaatttacctaaatttttagaaaatgctaaagcatttgaaaaaataaaa
caagtagaaagtttgcaagttaattttagagaattaatgggggaatttggagatgaaggg
ctaattttcgtaaatgaattagaagaaatgtttcaaatcaattattataatgatgtgctt
tcacaaaatggaattacaatttataatagtataatttcaggatttaccaaaaatgatata
aaatataaaggtctaaatgaatacataaataattacaatcaaaccaaagacaaaaaagac
cgtttgccaaaattaaaacaattgtataaacagattttgagtgataggatttcactttcg
tttttgcccgatgcttttacggatgggaaacaagttttgaaagccatatttgacttttat
aaaatcaacttactttcttataccattgaaggacaggaagaaagccaaaatcttttacta
ttaattcgtcagacaattgaaaacctttctagttttgatacccaaaaaatttatctaaaa
aatgatacccatttaaccactatttcacaacaagtatttggcgatttttcggtgttttca
actgctttaaattattggtatgaaactaaagtaaatccaaaatttgaaacggaatatagc
aaagccaacgaaaaaaaacgagaaattttagataaagccaaagcggtatttacaaaacaa
gattatttttcaattgcttttttacaagaagtactttcggaatacattcttaccttagat
cacacttctgatattgtaaaaaagcattcctccaactgtattgcggattattttaaaaat
cattttgtagccaaaaaagaaaatgaaaccgacaaaacctttgattttattgctaatatt
actgcaaaataccaatgtattcaaggtattttagaaaatgcagaccaatacgaagacgaa
ctcaaacaagaccaaaaattaattgataatttgaaattctttttagatgctattttagaa
ttgttgcattttattaaacctttgcatttaaaatcagaaagcattaccgaaaaagacact
gctttttatgatgtgtttgaaaattattacgaagcattgagtttgttgaccccattatat
aatatggtgcgaaactatgtaacgcaaaagccgtacagcaccgaaaaaataaaattaaat
tttgaaaatgcacaattattgaatggttgggatgccaataaagaaggtgattacctaact
accattttgaaaaaagacggtaattattttttagccataatggataaaaagcataacaaa
gcgtttcaaaagtttccagaaggaaaagaaaattatgaaaaaatggtgtataaactattg
cctggagtaaataagatgttgccaaaagtatttttttccaataaaaatattgcttacttc
aacccatcaaaagagttattagaaaactataaaaaagagacgcacaaaaaaggagacaca
ttcaatttagaacattgtcatacgttgatcgattttttcaaggactctttaaacaaacat
gaagactggaaatactttgattttcaattttctgaaacaaaatcgtatcaagatttgagt
ggtttttatagagaagtagaacatcaaggctacaaaatcaattttaaaaatatcgattca
gaatatattgatggtttggtgaacgaaggtaaattgtttctatttcaaatttacagcaaa
gatttttcgcctttttccaaagggaaaccgaacatgcacactttgtattggaaagcctta
tttgaagaacaaaatttgcaaaatgtaatctataaattgaatggacaagccgaaatattt
tttagaaaagcctctataaaacctaaaaatataatattgcacaaaaagaaaattaaaatt
gccaaaaagcattttattgataaaaaaacaaaaacatctgaaattgttcctgttcaaaca
ataaaaaacctcaatatgtactaccaaggaaaaataagtgaaaaagaattaacacaagat
gatttaaggtatattgataattttagcattttcaatgaaaaaaataaaacaattgatatt
ataaaagacaaacgatttacggttgataaatttcagtttcatgtgccgattaccatgaac
tttaaagcaacgggcggaagttatatcaatcaaaccgtattagaatatttgcaaaacaat
cccgaagttaagattattggattggatagaggcgaacgccatttggtatatctgacactg
atagaccagcaaggaaacatcttgaaacaagaaagtttgaatacaatcaccgattctaaa
atctcgacaccttatcataagttgttggataacaaggaaaacgagcgtgacttggctcga
aaaaattggggaacggtggaaaacatcaaagaactcaaagaaggctacatcagtcaagtg
gtgcataaaattgctacgttgatgctggaagaaaatgccattgtggtaatggaagatttg
aattttggatttaaacgtggacgttttaaagtggaaaaacaaatttatcaaaagctggaa
aaaatgttgattgacaaattgaattatttggttttaaaagacaaacaacctcaggaatta
ggcggattgtacaacgcattacaactcaccaataaatttgaaagtttccaaaaaatgggt
aaacaatcgggctttttgttttatgtacccgcttggaacacctccaaaatagacccaacc
acagggtttgtcaattatttttataccaaatatgaaaatgttgacaaagccaaagccttt
tttgaaaaatttgaggcgattcgtttcaatgcagaaaagaagtattttgaatttgaagta
aaaaaatatagcgattttaacccaaaagccgaaggcactcaacaagcctggaccatttgc
acgtatggcgaacgaatagaaaccaaacgacaaaaagaccaaaacaacaaatttgtaagc
actccaattaatctaaccgaaaagatagaagactttttgggtaaaaaccaaattgtttat
ggtgatggtaattgcatcaaatctcaaattgctagcaaagacgacaaggctttttttgaa
accttattgtattggttcaaaatgactttacaaatgcgaaacagcgaaacaagaacagat
atagattatctaatttcgcccgtgatgaatgacaacggaacattttacaacagccgagat
tatgaaaaattagaaaatccaactttgcccaaagatgccgatgccaacggagcgtatcat
attgccaaaaaaggattgatgcttttgaataaaatagaccaagccgacttgacaaaaaaa
gtggatttatctattagtaacagagattggttgcaatttgtacaaaaaaataaataa
SEQ atggaacaggagtactatttaggactggatatgggaaccggatctgtaggatgggctgtt
ID acagattcggaatatcatgtcttgcgtaaacatggaaaagcactatggggagtccgatta
NO: tttgaaagtgcatcgacagcagaagaacgaagaatgttccgaacatcaagaagaagacta
29 gatcgaagaaactggagaattgaaattttacaggaaatttttgcagaggaaataagtaag
aaagatccaggatttttcttgcgaatgaaagaaagcaaatattatccagaagataagcga
gatatcaatggaaattgtccggaactgccatatgcattatttgttgatgacgattttaca
gataaagattatcataaaaaatttccgacaatttatcatctcaggaaaatgttgatgaat
acagaggagacaccggatatccggttggtgtatctggcaattcatcatatgatgaagcat
aggggccatttcttgttatctggtgacattaatgagattaaggagttcggaacgacattt
tcaaaattgttggagaatatcaaaaatgaggaattggattggaatcttgaactgggaaaa
gaagaatatgctgttgtagaaagtattttaaaagataacatgttaaaccgatccacaaag
aaaaccagattaataaaagcattaaaagcaaaatcaatatgtgaaaaggctgtactgaat
ttattggctggtggaacggtgaaattgagtgatatatttggtcttgaagaattaaatgag
acagaaagaccgaagatttcctttgctgataatggatacgatgattatatcggagaagtt
gaaaatgagctgggagaacaattctatattatagagacggcaaaagcagtgtatgactgg
gcggtattagttgaaatattgggaaaatatacgtcaatttcagaagcgaaagtagcaacg
tatgaaaaacataaatcggatttacaatttttgaaaaagatagttcggaaatatctgaca
aaggaggaatataaagatatttttgtaagtacgagtgacaaattgaaaaattactctgct
tatataggaatgacgaaaataaatggaaaaaaggttgatttgcagagcaaacggtgcagt
aaagaagaattctatgattttattaagaaaaacgtacttaaaaagctagaaggacaacct
gaatatgaatatttgaaagaagagctagaaagagaaacatttctaccaaaacaggtgaac
agggataatggtgtaataccgtatcagattcatttgtacgagttgaaaaagatattagga
aatttacgggataaaatagacctcattaaagagaacgaagataaactggttcaattattt
gaattcagaattccgtattatgttggtccgctgaataagatagatgacggaaaagaggga
aaatttacatgggctgtacggaaaagtaatgaaaagatatatccatggaattttgaaaat
gtagttgatatagaagcaagtgcagaaaaatttatccggagaatgacaaataagtgtaca
tatctgatgggcgaagatgtattgccgaaggattcattgctttacagtaaatatatggtt
ttaaatgaattaaataatgtaaagttggatggcgaaaaattatctgtagaattgaaacaa
cggttgtatacagatgtattttgtaagtatcggaaagtaactgtaaagaagataaaaaat
tacttgaaatgtgaaggtatcatatccggcaatgtcgaaataactggaattgatggtgat
tttaaggcatcgttaacggcatatcatgattttaaagaaatcttgacaggaacagaattg
gctaaaaagga
caaagaaaatattattaccaatatagtattgtttggagatgataaaaagctgctgaaaaa
gagactgaatcgattatatcctcagattacgccgaatcagttgaagaaaatatgtgcgct
atcctatacaggctggggaagattttctaaaaagttcttagaagaaataacagctccaga
tccggaaacgggagaggtatggaatatcattacggcattgtgggaatcgaataataatct
gatgcaattattaagtaatgaatatcggtttatggaagaagtcgaaacatacaatatggg
aaaacagactaaaacattgtcgtacgaaacagtagagaatatgtatgtttctccatctgt
gaaaagacagatatggcagacgctgaaaatcgtgaaagaattagaaaaagtaatgaaaga
atctccgaaacgtgtatttattgagatggcgagagaaaagcaagaaagtaagagaaccga
atcgcgtaaaaaacaactaatagatttgtataaggcttgtaaaaatgaagaaaaagattg
ggtaaaagaactgggagatcaggaagaacagaaattacgaagcgataagttgtacctata
ttatacgcaaaagggtcgttgtatgtattctggcgaggtaatagaactgaaagacttatg
ggataatacaaaatatgatattgatcatatatatccacaatctaaaacgatggatgacag
tcttaataatcgcgtattggtaaaaaagaaatataatgcaacaaaatcagataagtatcc
attaaatgaaaatatacgacatgagagaaaaggcttttggaagtcactgttagatggagg
gtttataagtaaagaaaaatatgaacgcttaataagaaatacagaattgagtccggaaga
attagcaggatttattgaaaggcagattgttgaaacgaggcagagtacaaaagctgtagc
ggaaatattaaagcaagtgtttccggaaagtgaaattgtatatgtcaaagcaggtacggt
ttcaagattcagaaaagattttgaattactgaaagttcgagaagtgaatgatttgcatca
cgcaaaggatgcgtatttaaatattgtagttggtaatagttattatgtgaaatttactaa
gaatgcatcatggtttataaaagaaaatccgggacgtacttacaacttaaaaaagatgtt
tacatcaggttggaatattgaacgaaatggagaagttgcatgggaagtcgggaaaaaagg
aacaattgtaacggtaaaacaaataatgaataaaaataatatattggtgacaagacaggt
tcatgaagcgaaaggtgggctgtttgatcagcagattatgaaaaaaggaaaaggtcagat
tgctataaaggaaactgatgaacgtcttgcatcaatagaaaagtatggaggctataataa
agctgccggggcatattttatgctggtagaatctaaagataaaaaaggaaaaacaattcg
aacgatagaatttataccattatatttaaagaataaaatcgagtcggatgaatcaatagc
attgaactttttagaaaaaggcagaggtttgaaagaaccaaagatactattgaaaaaaat
taagattgatacattatttgatgtggacggattcaaaatgtggttgtctggaagaacagg
ggacagactactatttaaatgtgcaaatcaattgattttggatgagaaaataattgtaac
aatgaaaaaaattgtaaagtttattcaaaggagacaagaaaatagagaattaaaattatc
tgataaagatggaattgataatgaagtacttatggaaatatataacacttttgtggataa
gttagaaaacacagtgtatagaatacgattatccgaacaggcaaaaacgcttatagataa
acaaaaagaatttgaaaggttatcactagaggataaaagtagtactttgtttgaaatttt
acatatttttcagtgtcaaagtagtgcggccaatttaaaaatgataggcggacctggaaa
agcaggaatattagttatgaataataatataagtaagtgtaacaaaatttctattataaa
tcagtctccaacaggaattttcgaaaatgagattgatttgttaaagat
SEQ ATGAAATCTTTCGATTCATTCACAAATCTTTATTCTCTTTCAAAAACCTTGAAATTTGAG
ID ATGAGACCTGTCGGAAATACCCAAAAAATGCTCGACAATGCAGGAGTATTTGAAAAAGAC
NO: AAACTAATTCAAAAAAAGTACGGAAAAACAAAGCCGTATTTCGACAGACTCCACAGAGAA
30 TTTATAGAAGAAGCGCTCACGGGGGTAGAGCTAATAGGACTAGATGAGAACTTTAGGACA
CTTGTTGACTGGCAAAAAGATAAGAAAAATAATGTCGCAATGAAAGCGTATGAAAATAGT
TTGCAGCGGCTGAGAACGGAAATAGGTAAAATATTTAACCTAAAGGCTGAGGATTGGGTA
AAGAACAAATATCCAATATTAGGGCTGAAAAATAAAAATACCGATATTTTATTCGAAGAG
GCTGTATTCGGGATATTGAAAGCCCGATATGGAGAAGAAAAAGATACTTTTATAGAAGTA
GAGGAAATAGATAAAACCGGCAAATCAAAGATCAATCAAATATCAATTTTCGATAGTTGG
AAAGGATTTACAGGATATTTCAAAAAATTTTTTGAAACCAGAAAGAATTTTTACAAAAAC
GACGGAACTTCTACAGCAATTGCTACAAGGATCATTGATCAAAATCTGAAAAGATTCATA
GATAATCTGTCAATAGTTGAAAGTGTGAGACAAAAGGTTGATCTCGCCGAGACAGAAAAA
TCTTTCAGCATATCTCTATCGCAATTCTTCTCAATAGACTTTTATAACAAGTGTCTCCTT
CAAGATGGTATTGATTACTACAACAAGATAATCGGTGGAGAAACTCTCAAAAATGGCGAA
AAACTAATAGGTCTCAATGAACTAATAAATCAATATAGGCAGAATAATAAGGATCAGAAA
ATCCCATTTTTCAAACTTCTTGATAAACAAATTTTGAGTGAAAAGATATTATTTTTGGAT
GAAATAAAAAATGACACAGAACTGATCGAGGCGCTGAGTCAGTTCGCAAAAACAGCCGAA
GAAAAAACAAAAATTGTCAAAAAGCTTTTTGCCGATTTTGTAGAAAATAATTCCAAATAC
GATCTTGCACAGATTTATATTTCCCAAGAAGCATTCAATACTATATCAAACAAGTGGACA
AGCGAAACTGAGACGTTCGCTAAATATCTATTCGAAGCAATGAAGAGTGGAAAACTTGCA
AAGTATGAGAAAAAAGATAATAGCTATAAATTTCCTGATTTTATTGCCCTTTCACAGATG
AAGAGTGCTTTATTAAGTATCAGCCTTGAGGGACATTTTTGGAAAGAGAAATACTACAAA
ATTTCAAAATTCCAAGAGAAGACCAATTGGGAGCAGTTTCTTGCAATTTTTCTATACGAG
TTTAACTCTCTTTTCAGCGACAAAATAAATACAAAAGATGGAGAAACAAAGCAAGTTGGA
TACTATCTATTTGCCAAAGACCTGCATAATCTTATCTTAAGTGAGCAGATTGATATTCCA
AAAGATTCAAAAGTCACAATAAAAGATTTTGCCGATTCTGTACTCACAATCTACCAAATG
GCAAAATATTTTGCGGTAGAAAAAAAACGAGCGTGGCTTGCCGAGTATGAACTAGATTCA
TTTTATACCCAGCCAGACACAGGCTATTTACAGTTTTATGATAACGCCTACGAGGATATT
GTGCAGGTATACAACAAGCTTCGAAACTATCTGACCAAAAAGCCATATAGCGAGGAGAAA
TGGAAGTTGAATTTTGAAAATTCTACGCTGGCAAATGGATGGGATAAGAACAAAGAATCT
GATAATTCAGCAGTTATTCTACAAAAAGGTGGAAAATATTATTTGGGACTGATTACTAAA
GGACACAACAAAATTTTTGATGACCGTTTTCAAGAAAAATTTATTGTGGGAATTGAAGGT
GGAAAATATGAAAAAATAGTCTATAAATTTTTCCCCGACCAGGCAAAAATGTTTCCCAAA
GTGTGCTTTTCTGCAAAAGGACTCGAATTTTTTAGACCGTCTGAAGAAATTTTAAGAATT
TATAACAATGCAGAGTTTAAAAAAGGAGAAACTTATTCAATAGATAGTATGCAGAAGTTG
ATTGATTTTTATAAAGATTGCTTGACTAAATATGAAGGCTGGGCATGTTATACCTTTCGG
CATCTAAAACCCACAGAAGAATACCAAAACAATATTGGAGAGTTTTTTCGAGATGTTGCA
GAGGACGGATACAGGATTGATTTTCAAGGCATTTCAGATCAATATATTCATGAAAAAAAC
GAGAAAGGCGAACTTCACCTTTTTGAAATCCACAATAAAGATTGGAATTTGGATAAGGCA
CGAGACGGAAAGTCAAAAACAACACAAAAAAACCTTCATACACTCTATTTCGAATCGCTC
TTTTCAAACGATAATGTTGTTCAAAACTTTCCAATAAAACTCAATGGTCAAGCTGAAATT
TTTTATAGACCGAAAACGGAAAAAGACAAATTAGAATCAAAAAAAGATAAGAAAGGGAAT
AAAGTGATTGACCATAAACGCTATAGTGAGAATAAGATTTTTTTTCATGTTCCTCTCACA
CTAAACCGCACTAAAAATGACTCATATCGCTTTAATGCTCAAATCAACAACTTTCTCGCA
AATAATAAAGATATCAACATCATCGGTGTAGATAGGGGAGAAAAGCATTTAGTCTATTAT
TCGGTGATTACACAAGCTAGTGACATCTTAGAAAGTGGCTCACTAAATGAGCTAAATGGC
GTGAATTATGCTGAAAAACTGGGAAAAAAGGCAGAAAATCGAGAACAAGCACGCAGAGAC
TGGCAAGACGTACAAGGGATCAAAGACCTCAAGAAAGGATATATTTCACAGGTGGTGCGA
AAGCTTGCTGATTTAGCAATTAAACACAATGCCATTATCATTCTTGAAGATTTGAATATG
AGATTTAAACAAGTTCGGGGCGGTATCGAAAAATCCATTTATCAACAGTTAGAAAAAGCA
CTGATAGATAAATTAAGCTTTCTTGTAGACAAAGGTGAAAAAAATCCCGAGCAAGCAGGA
CATCTTCTGAAAGCATATCAGCTTTCGGCCCCATTTGAGACATTTCAAAAAATGGGCAAA
CAGACGGGTATAATCTTTTATACACAAGCTTCGTATACCTCAAAAAGTGACCCTGTAACA
GGTTGGCGACCACACCTGTATCTCAAATATTTCAGTGCCAAAAAAGCAAAAGACGATATT
GCAAAGTTTACAAAAATAGAATTTGTAAACGATAGGTTTGAGCTTACCTATGATATAAAG
GACTTTCAGCAAGCAAAAGAATATCCAAATAAAACTGTTTGGAAAGTTTGCTCAAATGTA
GAGAGATTCAGGTGGGACAAAAACCTCAATCAAAACAAAGGCGGATATACTCACTACACA
AATATAACTGAGAATATCCAAGAGCTTTTTACAAAATATGGAATTGATATCACAAAAGAT
TTGCTCACACAGATTTCTACAATTGATGAAAAACAAAATACCTCATTTTTTAGAGATTTT
ATTTTTTATTTCAACCTTATTTGCCAAATCAGAAATACCGATGATTCTGAGATTGCTAAA
AAGAATGGGAAAGATGATTTTATACTGTCACCTGTTGAGCCGTTTTTCGATAGCCGAAAA
GACAATGGAAATAAACTTCCTGAGAATGGAGATGATAACGGCGCGTATAACATAGCAAGA
AAAGGGATTGTCATACTCAACAAA
ATCTCACAATATTCAGAGAAAAACGAAAATTGCGAGAAAATGAAATGGGGGGATTTGTAT
GTATCAAACATTGACTGGGACAATTTTGTAACCCAAGCTAATGCACGGCATTAA
SEQ ATGATTATCTTATATATTAGTACCTCGAATATGAACATGGAAGGAGTATTTATGGAAAAT
ID TTTAAAAACTTGTATCCAATAAACAAAACACTTCGATTTGAATTAAGACCCTATGGAAAA
NO: ACATTGGAAAATTTTAAAAAATCCGGACTTTTAGAAAAAGATGCCTTTAAGGCAAATAGT
31 AGACGAAGTATGCAAGCTATAATCGATGAAAAATTCAAAGAGACTATCGAAGAACGCTTA
AAGTACACTGAATTCAGTGAATGTGATCTTGGAAACATGACATCAAAAGATAAAAAAATA
ACTGATAAAGCAGCTACAAATTTAAAAAAGCAAGTTATCTTATCTTTTGACGATGAAATA
TTTAATAATTACCTAAAACCTGATAAAAATATTGACGCATTATTTAAAAATGATCCTTCA
AATCCTGTAATCTCTACATTTAAAGGTTTTACGACATATTTTGTGAATTTTTTTGAAATT
CGAAAACATATTTTCAAGGGAGAATCATCAGGCTCAATGGCATACCGAATTATAGATGAA
AACCTGACAACATACTTGAATAATATTGAAAAAATAAAAAAACTGCCAGAAGAATTAAAA
TCACAGCTAGAAGGCATTGATCAGATTGATAAACTTAATAATTATAATGAGTTCATTACA
CAGTCAGGTATAACACACTATAATGAAATCATCGGCGGTATATCAAAATCAGAGAATGTC
AAAATACAGGGAATTAATGAAGGAATTAATCTATACTGTCAGAAGAACAAAGTTAAACTT
CCTCGACTGACTCCGCTATACAAAATGATATTATCAGACAGAGTTTCCAACTCTTTTGTA
TTAGACACTATTGAAAATGACACAGAATTAATTGAAATGATAAGTGATTTGATTAATAAG
ACTGAGATTTCGCAAGATGTTATAATGTCAGATATTCAAAATATTTTCATAAAATACAAA
CAACTTGGTAATTTGCCGGGTATCTCATATTCTTCAATAGTTAATGCTATTTGCTCGGAT
TATGACAACAATTTCGGAGATGGGAAGCGAAAAAAATCTTACGAAAATGATCGCAAAAAG
CATTTGGAGACTAATGTATACTCCATAAATTATATTTCTGAATTGCTTACAGATACCGAT
GTTTCATCAAATATCAAGATGAGATATAAAGAGCTTGAGCAAAATTATCAGGTTTGCAAA
GAAAATTTTAATGCCACAAACTGGATGAATATTAAAAATATAAAACAATCTGAAAAAACA
AACCTTATTAAAGATTTGTTAGATATACTTAAATCGATTCAACGTTTCTATGATTTGTTT
GATATTGTTGACGAAGATAAAAATCCAAGTGCTGAATTTTATACCTGGTTATCAAAAAAT
GCTGAAAAGCTTGACTTTGAATTCAATTCTGTATATAACAAGTCACGAAACTATCTCACC
AGGAAACAATACTCTGATAAAAAAATCAAGCTGAATTTTGATTCTCCAACATTGGCCAAA
GGGTGGGATGCTAACAAAGAAATAGATAACTCCACGATTATAATGCGTAAATTTAATAAT
GACAGAGGCGATTATGATTACTTCCTTGGCATATGGAATAAATCCACACCTGCAAATGAA
AAAATAATCCCACTGGAGGATAATGGATTATTCGAAAAAATGCAATATAAGCTGTATCCA
GATCCTAGTAAGATGTTACCGAAACAATTTCTATCAAAAATATGGAAGGCAAAGCATCCT
ACGACACCTGAATTTGATAAAAAATATAAAGAGGGAAGACATAAAAAAGGTCCTGATTTC
GAAAAAGAATTCCTGCATGAATTGATTGATTGCTTCAAACATGGTCTTGTTAATCACGAT
GAAAAATATCAGGATGTTTTTGGCTTCAATCTCCGTAACACTGAAGATTATAATTCATAT
ACAGAGTTTCTCGAAGATGTGGAAAGATGCAATTACAATCTTTCATTTAACAAAATTGCT
GATACTTCAAACCTTATTAATGATGGGAAATTGTATGTATTTCAGATATGGTCAAAAGAC
TTTTCTATTGATTCAAAAGGTACTAAAAACTTGAATACAATCTATTTTGAATCACTATTT
TCAGAAGAAAACATGATAGAAAAAATGTTCAAGCTTTCTGGAGAGGCTGAGATATTCTAT
CGACCAGCATCGTTGAATTATTGTGAAGATATCATAAAAAAAGGTCATCACCATGCAGAA
TTAAAAGATAAGTTTGACTATCCTATAATAAAAGATAAGCGATATTCACAAGATAAGTTT
TTCTTTCATGTGCCAATGGTTATAAATTATAAATCTGAGAAACTGAATTCCAAAAGCCTT
AACAACCGAACAAATGAAAACCTGGGACAGTTTACACATATTATAGGTATAGACAGGGGC
GAGCGGCACTTGATTTATTTAACTGTTGTTGATGTTTCCACTGGTGAAATCGTTGAACAG
AAACATCTGGACGAAATTATCAATACTGATACCAAGGGAGTTGAACACAAAACCCATTAT
TTGAATAAATTGGAAGAAAAATCTAAAACAAGAGATAACGAGCGTAAATCATGGGAAGCT
ATTGAAACTATCAAAGAATTAAAAGAAGGCTATATTTCTCATGTAATTAATGAAATACAA
AAGCTGCAAGAAAAATATAATGCCTTAATCGTAATGGAAAATCTTAACTATGGGTTCAAA
AACTCACGAATCAAAGTTGAAAAACAGGTTTATCAAAAATTCGAGACAGCATTGATTAAA
AAGTTCAATTATATTATTGATAAAAAAGATCCAGAAACCTATATACATGGTTACCAGCTT
ACAAATCCTATTACCACTCTGGATAAGATTGGAAATCAATCTGGAATAGTGCTGTATATT
CCTGCGTGGAATACTTCTAAGATAGATCCCGTCACAGGATTTGTAAACCTTCTGTACGCA
GATGATTTGAAGTATAAAAATCAGGAGCAGGCCAAATCATTCATTCAGAAAATAGACAAC
ATATATTTTGAAAATGGAGAGTTTAAATTTGATATTGATTTTTCCAAATGGAATAATCGC
TACTCAATAAGTAAAACTAAATGGACGTTAACAAGTTATGGGACTCGCATCCAGACATTT
AGAAATCCCCAGAAAAACAATAAGTGGGATTCTGCTGAATATGATTTGACAGAAGAGTTT
AAATTAATTTTAAATATAGACGGAACGTTAAAGTCACAGGACGTAGAAACATACAAAAAA
TTCATGTCTTTATTTAAACTAATGCTACAGCTTCGAAACTCTGTTACAGGAACCGACATT
GATTATATGATCTCTCCTGTCACTGATAAAACAGGAACACATTTCGATTCAAGAGAAAAT
ATTAAAAATCTTCCTGCCGATGCAGATGCCAATGGTGCCTACAACATTGCGCGCAAAGGA
ATAATGGCTATTGAAAATATAATGAACGGTATAAGCGATCCACTAAAAATAAGCAACGAA
GACTATTTAAAGTATATTCAGAATCAACAGGAATAA
SEQ ATGACCCAATTTGAAGGTTTTACCAATTTATACCAAGTTTCGAAGACCCTTCGTTTTGAA
ID CTGATTCCCCAAGGAAAAACACTCAAACATATCCAGGAGCAAGGGTTCATTGAGGAGGAT
NO: AAAGCTCGCAATGACCATTACAAAGAGTTAAAACCAATCATTGACCGCATCTATAAGACT
32 TATGCTGATCAATGTCTCCAACTGGTACAGCTTGACTGGGAGAATCTATCTGCAGCCATA
GACTCCTATCGTAAGGAAAAAACCGAAGAAACACGAAATGCGCTGATTGAGGAGCAAGCA
ACATATAGAAATGCGATTCATGACTACTTTATAGGTCGGACGGATAATCTGACAGATGCC
ATAAATAAGCGCCATGCTGAAATCTATAAAGGACTTTTTAAAGCTGAACTTTTCAATGGA
AAAGTTTTAAAGCAATTAGGGACCGTAACCACGACAGAACATGAAAATGCTCTACTCCGT
TCGTTTGACAAATTTACGACCTATTTTTCCGGCTTTTATGAAAACCGAAAAAATGTCTTT
AGCGCTGAAGATATCAGCACGGCAATTCCCCATCGAATCGTCCAGGACAATTTCCCTAAA
TTTAAGGAAAACTGCCATATTTTTACAAGATTGATAACCGCAGTTCCTTCTTTGCGGGAG
CATTTTGAAAATGTCAAAAAGGCCATTGGAATCTTTGTTAGTACGTCTATTGAAGAAGTC
TTTTCCTTTCCCTTTTATAATCAACTTCTAACCCAAACGCAAATTGATCTTTATAATCAA
CTTCTCGGCGGCATATCTAGGGAAGCAGGCACAGAAAAAATCAAGGGACTTAATGAAGTT
CTCAATCTGGCTATCCAAAAAAATGATGAAACAGCCCATATAATCGCGTCCCTGCCGCAT
CGTTTTATTCCTCTTTTTAAACAAATTCTTTCCGATCGAAATACGTTATCCTTTATTTTG
GAAGAATTCAAAAGCGATGAGGAAGTCATCCAATCCTTCTGCAAATATAAAACCCTCTTG
AGAAACGAAAATGTACTGGAGACTGCAGAAGCCCTTTTCAATGAATTAAATTCCATTGAT
TTGACTCATATCTTTATTTCCCATAAAAAGTTAGAAACCATCTCTTCAGCGCTTTGTGAC
CATTGGGATACCTTGCGCAATGCACTTTACGAAAGACGGATTTCTGAACTCACTGGCAAA
ATAACAAAAAGTGCCAAAGAAAAAGTTCAAAGGTCATTAAAACATGAGGATATAAATCTC
CAAGAAATTATTTCTGCTGCAGGAAAAGAACTATCAGAAGCATTCAAACAAAAAACAAGT
GAAATTCTTTCCCATGCCCATGCTGCACTTGACCAGCCTCTTCCCACAACATTAAAAAAA
CAGGAAGAAAAAGAAATCCTCAAATCACAGCTCGATTCGCTTTTAGGCCTTTATCATCTT
CTTGATTGGTTTGCTGTCGATGAAAGCAATGAAGTCGACCCAGAATTCTCAGCACGGCTG
ACAGGCATTAAACTAGAAATGGAACCAAGCCTTTCGTTTTATAATAAAGCAAGAAATTAT
GCGACAAAAAAGCCCTATTCGGTGGAAAAATTTAAATTGAATTTTCAAATGCCAACCCTT
GCCTCTGGTTGGGATGTCAATAAAGAAAAAAATAATGGAGCTATTTTATTCGTAAAAAAT
GGTCTCTATTACCTTGGTATCATGCCTAAACAGAAGGGGCGCTATAAAGCCCTGTCTTTT
GAGCCGACAGAAAAAACATCAGAAGGATTCGATAAGATGTACTATGACTACTTCCCAGAT
GCCGCAAAAATGATTCCTAAGTGTTCCACTCAGCTAAAGGCTGTAACCGCTCATTTTCAA
ACTCATACCACCCCCATTCTTCTCTCAAATAATTTCATTGAACCTCTTGAAATCACAAAA
GAAATTTATGACCTGAACAATCCTGAAAAGGAGCCTAAAAAGTTTCAAACGGCTTATGCA
AAGAAGACAGGCGATCAAAAAGGCTATAGAGAAGCGCTTTGCAAATGGATTGACTTTACG
CGGGATTTTCTCTCTAAATATACGAAAACAACTTCAATCGATTTATCTTCACTCCGCCCT
TCTTCGCAATATAAAGATTTAGGGGAATATTACGCCGAACTGAATCCGCTTCTCTATCAT
ATCTCCTTCCAACGAATTGCTGAAAAGGAAATCATGGATGCTGTAGAAACGGGAAAATTG
TATCTGTTCCAAATCTACAATAAGGATTTTGCGAAGGGCCATCACGGGAAACCAAATCTC
CACACCCTGTATTGGACAGGTCTCTTCAGTCCTGAAAACCTTGCGAAAACCAGCATCAAA
CTTAATGGTCAAGCAGAATTGTTCTATCGACCTAAAAGCCGCATGAAGCGGATGGCCCAT
CGTCTTGGGGAAAAAATGCTGAACAAAAAACTAAAGGACCAGAAGACACCGATTCCAGAT
ACCCTCTACCAAGAACTGTACGATTATGTCAACCACCGGCTAAGCCATGATCTTTCCGAT
GAAGCAAGGGCCCTGCTTCCAAATGTTATCACCAAAGAAGTCTCCCATGAAATTATAAAG
GATCGGCGGTTTACTTCCGATAAATTTTTCTTCCATGTTCCCATTACACTGAATTATCAA
GCAGCCAATAGTCCCAGTAAATTCAACCAGCGTGTCAATGCCTACCTTAAGGAGCATCCG
GAAACGCCCATCATTGGTATCGATCGTGGAGAACGCAATCTAATCTATATTACCGTCATT
GACAGTACTGGGAAAATTTTGGAGCAGCGTTCCCTGAATACCATCCAGCAATTTGACTAC
CAAAAAAAATTGGACAACAGGGAAAAAGAGCGTGTTGCCGCCCGTCAAGCCTGGTCCGTC
GTCGGAACGATCAAAGACCTTAAACAAGGCTACTTGTCACAGGTCATCCATGAAATTGTA
GACCTGATGATTCATTACCAAGCTGTTGTCGTCCTTGAAAACCTCAACTTCGGATTTAAA
TCAAAACGGACAGGCATTGCCGAAAAAGCAGTCTACCAACAATTTGAAAAGATGCTAATA
GATAAACTCAACTGTTTGGTTCTCAAAGATTATCCTGCTGAGAAAGTGGGAGGCGTCTTA
AACCCGTATCAACTTACAGATCAGTTCACGAGCTTTGCAAAAATGGGCACGCAAAGCGGC
TTCCTTTTCTATGTACCGGCCCCTTATACCTCAAAGATTGATCCCCTGACTGGTTTTGTC
GATCCCTTTGTATGGAAGACCATTAAAAATCATGAAAGTCGGAAGCATTTCCTAGAAGGA
TTTGATTTCCTGCATTATGATGTCAAAACAGGTGATTTTATCCTCCATTTTAAAATGAAT
CGGAATCTCTCTTTCCAGAGAGGGCTTCCTGGCTTCATGCCAGCTTGGGATATTGTTTTC
GAAAAGAATGAAACCCAATTTGATGCAAAAGGGACGCCCTTCATTGCAGGAAAACGAATT
GTTCCTGTAATCGAAAATCATCGTTTTACGGGTCGTTACAGAGACCTCTATCCCGCTAAT
GAACTCATTGCCCTTCTGGAAGAAAAAGGCATTGTCTTTAGAGACGGAAGTAATATATTA
CCCAAACTTTTAGAAAATGATGATTCTCATGCAATTGATACGATGGTCGCCTTGATTCGC
AGTGTACTCCAAATGAGAAACAGCAATGCCGCAACGGGGGAAGACTACATCAACTCTCCC
GTTAGGGATCTGAACGGGGTGTGTTTCGACAGTCGATTCCAAAATCCAGAATGGCCAATG
GATGCGGATGCCAACGGAGCTTATCATATTGCCTTAAAAGGGCAGCTTCTTCTGAACCAC
CTCAAAGAAAGCAAAGATCTGAAATTACAAAACGGCATCAGCAACCAAGATTGGCTGGCC
TACATTCAGGAACTGAGAAACTGA
SEQ ATGGCCGTCAAATCCATCAAAGTGAAACTTCGTCTCGACGATATGCCGGAGATTCGGGCC
ID GGTCTATGGAAACTTCATAAGGAAGTCAATGCGGGGGTTCGATATTACACGGAATGGCTC
NO: AGTCTTCTCCGTCAAGAGAACTTGTATCGAAGAAGTCCGAATGGGGACGGAGAGCAAGAA
33 TGTGATAAGACTGCAGAAGAATGCAAAGCCGAATTGTTGGAGCGGCTGCGCGCGCGTCAA
GTGGAGAATGGACACCGTGGTCCGGCGGGATCGGACGATGAATTGCTGCAGTTGGCGCGT
CAACTCTATGAGTTGTTGGTTCCGCAGGCGATAGGTGCGAAAGGCGACGCGCAGCAAATT
GCCCGCAAATTTTTGAGCCCCTTGGCCGACAAGGACGCAGTTGGTGGGCTTGGAATCGCG
AAGGCGGGGAACAAACCGCGGTGGGTTCGCATGCGCGAAGCGGGGGAACCAGGCTGGGAA
GAGGAGAAGGAGAAGGCTGAGACGAGGAAATCTGCGGATCGGACTGCGGATGTTTTGCGC
GCGCTCGCGGATTTTGGGTTAAAGCCACTGATGCGCGTATACACCGATTCTGAGATGTCA
TCGGTGGAGTGGAAACCGCTTCGGAAGGGACAAGCCGTTCGGACGTGGGATAGGGACATG
TTCCAACAAGCTATCGAACGGATGATGTCGTGGGAGTCGTGGAATCAGCGCGTTGGGCAA
GAGTACGCGAAACTCGTAGAACAAAAAAATCGATTTGAGCAGAAGAATTTCGTCGGCCAG
GAACATCTGGTCCATCTCGTCAATCAGTTGCAACAAGATATGAAAGAAGCATCGCCCGGA
CTCGAATCGAAAGAGCAAACCGCGCACTATGTGACGGGACGGGCATTGCGCGGATCGGAC
AAGGTATTTGAGAAGTGGGGGAAACTCGCCCCCGATGCACCTTTCGATTTGTACGACGCC
GAAATCAAGAATGTGCAGAGACGTAACACGAGACGATTCGGATCACATGACTTGTTCGCA
AAATTGGCAGAGCCAGAGTATCAGGCCCTGTGGCGCGAAGATGCTTCGTTTCTCACGCGT
TACGCGGTGTACAACAGCATCCTTCGCAAACTGAATCACGCCAAAATGTTCGCGACGTTT
ACTTTGCCGGATGCAACGGCGCACCCGATTTGGACTCGCTTCGATAAATTGGGTGGGAAT
TTGCACCAGTACACCTTTTTGTTCAACGAATTTGGAGAACGCAGGCACGCGATTCGTTTT
CACAAGCTATTGAAAGTCGAGAATGGTGTCGCAAGAGAAGTTGATGATGTCACCGTGCCC
ATTTCAATGTCAGAGCAATTGGATAATCTGCTTCCCAGAGATCCCAATGAACCGATTGCG
CTATATTTTCGAGATTACGGAGCCGAACAGCATTTCACAGGTGAATTTGGTGGCGCGAAG
ATCCAGTGCCGCCGGGATCAGCTGGCTCATATGCACCGACGCAGAGGGGCGAGGGATGTT
TATCTCAATGTCAGCGTACGTGTGCAGAGTCAGTCTGAGGCGCGGGGAGAACGTCGCCCG
CCGTATGCGGCAGTATTTCGTCTGGTCGGGGACAACCATCGCGCGTTTGTCCATTTCGAT
AAACTATCGGATTATCTTGCGGAACATCCGGATGATGGGAAGCTCGGGTCGGAGGGGTTG
CTTTCCGGGCTGCGGGTGATGAGTGTCGATCTCGGCCTTCGCACATCTGCATCGATTTCC
GTTTTTCGCGTTGCCCGGAAGGACGAGTTGAAGCCGAACTCAAAAGGTCGTGTACCGTTT
TTCTTTCCGATAAAAGGGAATGACAATCTCGTCGCGGTTCATGAGCGATCACAACTCTTG
AAGCTGCCTGGCGAAACGGAGTCGAAGGACCTGCGTGCTATCCGAGAAGAACGCCAACGG
ACATTGCGGCAGTTGCGGACGCAACTGGCGTATTTGCGGCTGCTCGTGCGGTGTGGGTCG
GAAGATGTGGGGCGGCGTGAACGGAGTTGGGCAAAGCTTATCGAGCAGCCGGTGGATGCG
GCCAATCACATGACACCGGATTGGCGCGAGGCTTTTGAAAACGAACTTCAGAAGCTTAAG
TCACTCCATGGTATCTGTAGCGACAAGGAATGGATGGATGCTGTCTACGAGAGCGTTCGC
CGCGTGTGGCGTCACATGGGCAAACAGGTTCGCGATTGGCGAAAGGACGTACGAAGCGGA
GAGCGGCCCAAGATTCGCGGCTATGCGAAAGACGTGGTCGGTGGAAACTCGATTGAGCAA
ATCGAGTATCTGGAACGTCAGTACAAGTTCCTCAAGAGTTGGAGCTTCTTTGGTAAGGTG
TCGGGACAAGTGATTCGTGCGGAGAAGGGATCTCGTTTTGCGATCACGCTGCGCGAACAC
ATTGATCACGCGAAGGAAGATCGGCTGAAGAAATTGGCGGATCGCATCATTATGGAGGCT
CTCGGCTATGTGTACGCGTTGGATGAGCGCGGCAAAGGAAAGTGGGTTGCGAAGTATCCG
CCGTGCCAGCTCATCCTGCTGGAGGAATTGAGCGAGTACCAGTTCAATAACGACAGGCCT
CCGAGCGAAAACAACCAGTTGATGCAATGGAGTCATCGCGGCGTGTTCCAGGAGTTGATA
AATCAGGCCCAAGTCCATGATTTACTCGTTGGGACGATGTATGCAGCGTTCTCGTCGCGA
TTCGACGCGCGAACTGGGGCACCGGGTATCCGCTGTCGCCGGGTTCCGGCGCGTTGCACC
CAGGAGCACAATCCAGAACCATTTCCTTGGTGGCTGAACAAGTTTGTGGTGGAACATACG
TTGGATGCTTGTCCCCTACGCGCAGACGACCTCATCCCAACGGGTGAAGGAGAGATTTTT
GTCTCGCCGTTCAGCGCGGAGGAGGGGGACTTTCATCAGATTCACGCCGACCTGAATGCG
GCGCAAAATCTGCAGCAGCGACTCTGGTCTGATTTTGATATCAGTCAAATTCGGTTGCGG
TGTGATTGGGGTGAAGTGGACGGTGAACTCGTTCTGATCCCAAGGCTTACAGGAAAACGA
ACGGCGGATTCATATAGCAACAAGGTGTTTTATACCAATACAGGTGTCACCTATTATGAG
CGAGAGCGGGGGAAGAAGCGGAGAAAGGTTTTCGCGCAAGAGAAATTGTCGGAGGAAGAG
GCGGAGTTGCTCGTGGAAGCAGACGAGGCGAGGGAGAAATCGGTCGTTTTGATGCGTGAT
CCGTCTGGCATCATCAATCGGGGAAATTGGACCAGGCAAAAGGAATTTTGGTCGATGGTG
AACCAGCGGATCGAAGGATACTTGGTCAAGCAGATTCGCTCGCGCGTTCCATTACAAGAT
AGTGCGTGTGAAAACACGGGGGATATTTAA
SEQ ATGGCGACACGCAGTTTTATTTTAAAAATTGAACCAAATGAAGAAGTTAAAAAGGGATTA
ID TGGAAGACGCATGAGGTATTGAATCATGGAATTGCCTACTACATGAATATTCTGAAACTA
NO: ATTAGACAGGAAGCTATTTATGAACATCATGAACAAGATCCTAAAAATCCGAAAAAAGTT
34 TCAAAAGCAGAAATACAAGCCGAGTTATGGGATTTTGTTTTAAAAATGCAAAAATGTAAT
AGTTTTACACATGAAGTTGACAAAGATGTTGTTTTTAACATCCTGCGTGAACTATATGAA
GAGTTGGTCCCTAGTTCAGTCGAGAAAAAGGGTGAAGCCAATCAATTATCGAATAAGTTT
CTGTACCCGCTAGTTGATCCGAACAGTCAAAGTGGGAAAGGGACGGCATCATCCGGACGT
AAACCTCGGTGGTATAATTTAAAAATAGCAGGCGACCCATCGTGGGAGGAAGAAAAGAAA
AAATGGGAAGAGGATAAAAAGAAAGATCCCCTTGCTAAAATCTTAGGTAAGTTAGCAGAA
TATGGGCTTATTCCGCTATTTATTCCATTTACTGACAGCAACGAACCAATTGTAAAAGAA
ATTAAATGGATGGAAAAAAGTCGTAATCAAAGTGTCCGGCGACTTGATAAGGATATGTTT
ATCCAAGCATTAGAGCGTTTTCTTTCATGGGAAAGCTGGAACCTTAAAGTAAAGGAAGAG
TATGAAAAAGTTGAAAAGGAACACAAAACACTAGAGGAAAGGATAAAAGAGGACATTCAA
GCATTTAAATCCCTTGAACAATATGAAAAAGAACGGCAGGAGCAACTTCTTAGAGATACA
TTGAATACAAATGAATACCGATTAAGCAAAAGAGGATTACGTGGTTGGCGTGAAATTATC
CAAAAATGGCTAAAGATGGATGAAAATGAACCATCAGAAAAATATTTAGAAGTATTTAAA
GATTATCAACGGAAACATCCACGAGAAGCCGGGGACTATTCTGTCTATGAATTTTTAAGC
AAGAAAGAAAATCATTTTATTTGGCGAAATCATCCTGAATATCCTTATTTGTATGCTACA
TTTTGTGAAATTGACAAAAAAAAGAAAGACGCTAAGCAACAGGCAACTTTTACTTTGGCT
GACCCGATTAACCATCCGTTATGGGTACGATTTGAAGAAAGAAGCGGTTCGAACTTAAAC
AAATATCGAATTTTAACAGAGCAATTACACACTGAAAAGTTAAAAAAGAAATTAACAGTT
CAACTTGATCGTTTAATTTATCCAACTGAATCCGGCGGTTGGGAGGAAAAAGGTAAAGTA
GATATCGTTTTGTTGCCGTCAAGACAATTTTATAATCAAATCTTCCTTGATATAGAAGAA
AAGGGGAAACATGCTTTTACTTATAAGGATGAAAGTATTAAATTCCCCCTTAAAGGTACA
CTTGGTGGTGCAAGAGTGCAGTTTGACCGTGACCATTTGCGGAGATATCCGCATAAAGTA
GAATCAGGAAATGTTGGACGGATTTATTTTAACATGACAGTAAATATTGAACCAACTGAG
AGCCCTGTTAGTAAGTCTTTGAAAATACATAGGGACGATTTCCCCAAGTTCGTTAATTTT
AAACCGAAAGAGCTCACCGAATGGATAAAAGATAGTAAAGGGAAAAAATTAAAAAGTGGT
ATAGAATCCCTTGAAATTGGTCTACGGGTGATGAGTATCGACTTAGGTCAACGTCAAGCG
GCTGCTGCATCGATTTTTGAAGTAGTTGATCAGAAACCGGATATTGAAGGGAAGTTATTT
TTTCCAATCAAAGGAACTGAGCTTTATGCTGTTCACCGGGCAAGTTTTAACATTAAATTA
CCGGGTGAAACATTAGTAAAATCACGGGAAGTATTGCGGAAAGCTCGGGAGGACAACTTA
AAATTAATGAATCAAAAGTTAAACTTTCTAAGAAATGTTCTACATTTCCAACAGTTTGAA
GATATCACAGAAAGAGAGAAGCGTGTAACTAAATGGATTTCTAGACAAGAAAATAGTGAT
GTTCCTCTTGTATATCAAGATGAGCTAATTCAAATTCGTGAATTAATGTATAAACCCTAT
AAAGATTGGGTTGCCTTTTTAAAACAACTCCATAAACGGCTAGAAGTCGAGATTGGCAAA
GAGGTTAAGCATTGGCGAAAATCATTAAGTGACGGGAGAAAAGGTCTTTACGGAATCTCC
CTAAAAAATATTGATGAAATTGATCGAACAAGGAAATTCCTTTTAAGATGGAGCTTACGT
CCAACAGAACCTGGGGAAGTAAGACGCTTGGAACCAGGACAGCGTTTTGCGATTGATCAA
TTAAACCACCTAAATGCATTAAAAGAAGATCGATTAAAAAAGATGGCAAATACGATTATC
ATGCATGCCTTAGGTTACTGTTATGATGTAAGAAAGAAAAAGTGGCAGGCAAAAAATCCA
GCATGTCAAATTATTTTATTTGAAGATTTATCTAACTACAATCCTTACGAGGAAAGGTCC
CGTTTTGAAAACTCAAAACTGATGAAGTGGTCACGGAGAGAAATTCCACGACAAGTCGCC
TTACAAGGTGAAATTTACGGATTACAAGTTGGGGAAGTAGGTGCCCAATTCAGTTCAAGA
TTCCATGCGAAAACCGGGTCGCCGGGAATTCGTTGCAGTGTTGTAACGAAAGAAAAATTG
CAGGATAATCGCTTTTTTAAAAATTTACAAAGAGAAGGACGACTTACTCTTGATAAAATC
GCAGTTTTAAAAGAAGGAGACTTATATCCAGATAAAGGTGGAGAAAAGTTTATTTCTTTA
TCAAAGGATCGAAAGTTGGTAACTACGCATGCTGATATTAACGCGGCCCAAAATTTACAG
AAGCGTTTTTGGACAAGAACACATGGATTTTATAAAGTTTACTGCAAAGCCTATCAGGTT
GATGGACAAACTGTTTATATTCCGGAGAGCAAGGACCAAAAACAAAAAATAATTGAAGAA
TTTGGGGAAGGCTATTTTATTTTAAAAGATGGTGTATATGAATGGGGTAATGCGGGGAAA
CTAAAAATTAAAAAAGGTTCCTCTAAACAATCATCGAGTGAATTAGTAGATTCGGACATA
CTGAAAGATTCATTTGATTTAGCAAGTGAACTTAAGGGAGAGAAACTCATGTTATATCGA
GATCCGAGTGGAAACGTATTTCCTTCCGACAAGTGGATGGCAGCAGGAGTATTTTTTGGC
AAATTAGAAAGAATATTGATTTCTAAGTTAACAAATCAATACTCAATATCAACAATAGAA
GATGATTCTTCAAAACAATCAATGTAA
SEQ ATGCCCACCCGCACCATCAATCTGAAACTTGTTCTTGGGAAAAATCCTGAAAACGCAACA
ID TTGCGACGCGCCCTATTTTCGACACACCGTTTGGTTAACCAAGCGACGAAACGTATTGAG
NO: GAATTCTTGTTGCTGTGTCGTGGAGAAGCCTACAGAACAGTGGATAATGAGGGGAAGGAA
35 GCCGAGATTCCACGTCATGCAGTCCAAGAAGAAGCTCTTGCCTTTGCCAAAGCTGCTCAA
CGCCACAACGGCTGTATATCCACCTATGAAGACCAAGAGATTCTTGATGTACTGCGGCAA
CTGTACGAACGTCTTGTTCCTTCGGTCAACGAAAACAACGAGGCAGGCGATGCTCAAGCT
GCTAACGCCTGGGTCAGTCCGCTCATGTCGGCAGAAAGCGAAGGAGGCTTGTCGGTCTAC
GACAAGGTGCTTGATCCACCGCCGGTTTGGATGAAGCTTAAAGAAGAAAAGGCTCCAGGA
TGGGAAGCCGCTTCTCAAATTTGGATTCAGAGTGATGAGGGACAGTCGTTACTTAATAAG
CCAGGTAGCCCTCCCCGCTGGATTCGAAAACTGCGATCTGGGCAACCGTGGCAAGATGAT
TTCGTCAGTGACCAAAAGAAAAAGCAAGATGAGCTGACCAAAGGGAACGCACCACTTATA
AAACAACTCAAAGAAATGGGGTTGTTGCCTCTTGTTAACCCATTTTTTAGACATCTTCTT
GACCCTGAAGGTAAAGGCGTGAGTCCATGGGACCGTCTTGCTGTACGCGCTGCAGTGGCT
CACTTTATCTCCTGGGAAAGTTGGAATCATAGAACACGTGCAGAATACAATTCCTTGAAA
CTACGGCGAGACGAGTTTGAGGCAGCATCCGACGAATTCAAAGACGATTTTACTTTGCTC
CGACAATATGAAGCCAAACGCCATAGTACATTGAAAAGCATCGCGCTGGCCGACGATTCG
AACCCTTACCGGATTGGAGTACGTTCTCTGCGTGCCTGGAACCGCGTTCGTGAAGAATGG
ATAGACAAGGGTGCAACAGAAGAACAACGCGTGACCATATTGTCAAAGCTTCAAACACAA
CTTCGGGGAAAATTCGGCGATCCCGATCTGTTCAACTGGCTAGCTCAGGATAGGCATGTC
CATTTGTGGTCTCCTCGGGACAGCGTGACACCATTGGTTCGCATCAATGCGGTAGATAAA
GTTCTGCGTCGACGAAAACCGTATGCATTGATGACCTTTGCCCATCCCCGCTTCCACCCT
CGATGGATACTGTACGAGGCTCCAGGAGGAAGCAATCTCCGTCAATATGCATTGGATTGT
ACAGAAAACGCTCTACACATCACGTTGCCTTTGCTTGTCGACGATGCGCACGGAACCTGG
ATTGAAAAAAAGATCAGGGTGCCGCTGGCACCATCCGGACAAATTCAAGATTTAACTCTG
GAAAAACTTGAGAAGAAAAAAAATCGTTTATACTACCGTTCCGGTTTTCAGCAGTTTGCC
GGCTTGGCTGGCGGAGCTGAGGTTCTTTTCCACAGACCCTATATGGAACACGACGAACGC
AGCGAGGAGTCTCTTTTGGAACGTCCGGGAGCCGTTTGGTTCAAATTGACCCTGGATGTG
GCAACACAGGCTCCCCCGAACTGGCTTGATGGTAAGGGCCGTGTCCGTACACCGCCGGAG
GTACATCATTTTAAAACCGCATTGTCGAATAAAAGCAAACATACACGTACGCTGCAGCCG
GGTCTCCGTGTCTTGTCAGTAGACTTGGGCATGCGAACATTCGCCTCCTGCTCAGTATTT
GAACTCATCGAGGGAAAGCCTGAGACAGGCCGTGCCTTCCCTGTTGCCGATGAGAGATCA
ATGGACAGCCCGAATAAACTGTGGGCCAAGCATGAACGTAGTTTTAAACTGACGCTCCCC
GGCGAAACCCCTTCTCGAAAGGAAGAGGAAGAGCGTAGCATAGCAAGAGCGGAAATTTAT
GCACTGAAACGCGACATACAACGCCTCAAAAGCCTACTCCGCTTAGGTGAAGAAGATAAC
GATAACCGTCGTGATGCATTGCTTGAACAGTTCTTTAAAGGATGGGGAGAAGAAGACGTT
GTGCCTGGACAAGCGTTTCCACGCTCTCTTTTCCAAGGGTTGGGAGCTGCCCCGTTTCGC
TCAACTCCAGAGTTATGGCGTCAGCATTGCCAAACATATTATGACAAAGCGGAAGCCTGT
CTGGCTAAACATATCAGTGATTGGCGCAAGCGAACTCGTCCCCGTCCGACATCGCGGGAG
ATGTGGTACAAAACACGTTCCTATCATGGCGGCAAGTCCATTTGGATGTTGGAATATCTT
GATGCCGTTCGAAAACTGCTTCTCAGTTGGAGCTTACGTGGTCGTACTTACGGTGCCATT
AATCGCCAGGATACAGCCCGGTTTGGTTCTTTGGCATCACGGCTGCTCCACCATATCAAT
TCCCTAAAGGAAGACCGCATCAAAACAGGAGCCGACTCTATCGTTCAGGCTGCTCGCGGG
TATATTCCTCTCCCTCATGGCAAGGGTTGGGAACAAAGATATGAGCCTTGTCAGCTCATA
TTATTTGAAGACCTCGCACGATATCGCTTTCGCGTGGATCGACCTCGTCGAGAGAACAGC
CAACTCATGCAGTGGAACCATCGAGCCATCGTGGCAGAAACAACGATGCAAGCCGAACTC
TACGGACAAATTGTCGAAAATACTGCAGCGGGGTTCAGCAGTCGTTTTCACGCGGCGACA
GGTGCCCCCGGTGTACGTTGTCGTTTTCTTCTAGAAAGAGACTTTGATAACGATTTGCCC
AAACCGTACCTTCTCAGGGAACTTTCTTGGATGCTCGGCAATACAAAAGTCGAGTCTGAA
GAAGAAAAGCTTCGATTGCTGTCTGAAAAAATCAGGCCAGGCAGTCTTGTTCCTTGGGAT
GGAGGCGAACAGTTCGCTACCCTGCATCCCAAAAGACAAACACTTTGCGTCATTCATGCC
GATATGAATGCTGCCCAAAATTTACAACGCCGGTTTTTCGGTCGATGCGGCGAGGCCTTT
CGGCTTGTTTGTCAACCCCACGGTGACGACGTGTTACGACTCGCATCCACCCCAGGAGCT
CGTCTTCTTGGAGCCCTGCAGCAGCTTGAAAATGGACAAGGAGCTTTCGAGTTGGTTCGA
GACATGGGGTCAACAAGTCAAATGAACCGGTTCGTCATGAAGTCTTTGGGAAAAAAGAAA
ATAAAACCCCTTCAGGACAACAATGGAGACGACGAGCTTGAAGACGTGTTGTCCGTACTC
CCGGAGGAAGACGACACAGGACGTATCACAGTCTTCCGCGATTCATCAGGAATCTTTTTT
CCTTGCAACGTCTGGATACCGGCCAAACAGTTTTGGCCAGCAGTACGCGCCATGATTTGG
AAGGTCATGGCTTCCCATTCTTTGGGGTGA
SEQ ATGACAAAGTTAAGACACCGACAGAAAAAATTAACACACGACTGGGCTGGCTCCAAAAAG
ID AGGGAAGTATTAGGCTCAAATGGCAAGCTTCAGAATCCGTTGTTAATGCCGGTTAAAAAA
NO: GGTCAGGTTACTGAGTTCCGGAAAGCGTTTTCTGCGTATGCTCGCGCAACGAAAGGAGAA
36 ATGACTGACGGCCGAAAGAATATGTTTACGCATAGTTTCGAGCCATTTAAGACAAAGCCC
TCGCTTCATCAGTGTGAATTGGCAGATAAAGCATATCAATCTTTACATTCGTATCTGCCT
GGTTCTCTTGCTCATTTTCTATTATCTGCTCACGCATTAGGTTTTCGTATTTTTTCAAAA
TCTGGTGAAGCAACTGCATTCCAGGCATCCTCTAAAATTGAAGCTTACGAATCAAAATTG
GCAAGCGAATTAGCTTGTGTAGATTTATCTATTCAAAACTTGACTATTTCAACGCTTTTT
AATGCGCTTACAACGTCTGTAAGAGGGAAGGGCGAAGAAACTAGCGCTGACCCCTTAATT
GCACGATTTTACACCTTACTTACTGGCAAGCCTCTGTCTCGAGACACTCAAGGGCCTGAA
CGTGATTTAGCAGAAGTTATCTCGCGTAAGATAGCTAGTTCTTTTGGCACATGGAAAGAA
ATGACGGCAAACCCTCTTCAGTCATTACAATTTTTTGAAGAGGAACTCCATGCGCTGGAT
GCCAATGTCTCGCTCTCACCCGCCTTCGACGTTTTAATTAAAATGAATGATTTGCAGGGC
GATTTAAAAAATCGAACCATTGTTTTTGATCCTGACGCCCCTGTTTTTGAATATAACGCA
GAAGACCCTGCCGACATAATTATTAAACTTACAGCTCGTTACGCTAAAGAAGCTGTCATC
AAAAATCAAAACGTAGGAAATTACGTTAAAAACGCTATTACTACCACAAATGCCAATGGT
CTTGGTTGGCTTTTGAACAAAGGTTTGTCGTTACTCCCTGTCTCGACCGATGACGAATTG
CTAGAGTTTATTGGCGTTGAACGATCTCATCCCTCATGCCATGCCTTAATTGAATTGATT
GCACAATTAGAAGCCCCCGAGCTCTTTGAGAAGAACGTATTTTCAGATACTCGTTCTGAA
GTTCAAGGTATGATTGATTCAGCTGTTTCTAATCATATTGCTCGTCTTTCCAGCTCTAGA
AATAGCTTGTCAATGGATAGTGAAGAATTAGAACGTTTAATCAAAAGCTTTCAGATACAC
ACACCTCATTGCTCACTTTTTATTGGCGCCCAATCACTTTCACAGCAGTTAGAATCTTTG
CCTGAAGCCCTTCAATCGGGCGTTAATTCAGCCGATATTTTACTAGGCTCTACTCAATAT
ATGCTCACCAATTCTTTGGTTGAAGAGTCAATTGCAACTTATCAAAGAACACTTAATCGC
ATCAATTACTTGTCAGGTGTTGCAGGTCAGATTAACGGCGCAATAAAGCGAAAAGCGATA
GATGGAGAAAAAATTCACTTGCCTGCAGCTTGGTCAGAGTTGATATCTTTACCATTTATA
GGCCAGCCTGTTATAGATGTTGAAAGCGATTTAGCTCATCTAAAAAATCAATACCAAACA
CTTTCAAATGAGTTTGATACTCTTATATCTGCTTTGCAAAAGAATTTTGATTTGAACTTT
AATAAAGCGCTCCTTAATCGTACTCAGCATTTTGAAGCCATGTGTAGAAGCACTAAGAAA
AACGCTTTATCCAAACCAGAGATCGTTTCCTATCGCGACCTGCTTGCTCGATTAACTTCT
TGTTTGTATCGAGGCTCTTTAGTTTTGCGTCGTGCCGGCATTGAAGTGTTAAAAAAACAT
AAAATATTTGAGTCAAACAGCGAACTTCGTGAACATGTTCATGAAAGAAAGCATTTCGTG
TTTGTTAGTCCTCTAGATCGCAAAGCCAAGAAACTCCTTCGATTAACTGATTCGCGTCCA
GACTTGTTACATGTTATTGATGAAATATTGCAGCACGATAATCTTGAAAACAAAGACCGC
GAGTCACTTTGGCTAGTTCGCTCTGGTTATTTGCTTGCAGGACTTCCAGATCAACTTTCT
TCATCTTTTATTAACTTGCCTATCATTACTCAAAAAGGAGATAGACGCCTTATAGACCTG
ATTCAGTATGATCAAATTAATCGTGATGCTTTTGTTATGTTAGTGACCTCTGCATTCAAG
TCTAATTTGTCTGGTCTGCAGTATCGTGCCAATAAGCAATCGTTCGTTGTTACTCGCACG
CTAAGCCCTTATCTCGGCTCAAAACTTGTCTACGTACCCAAGGATAAAGATTGGTTAGTT
CCTTCTCAAATGTTTGAAGGACGATTTGCTGACATTCTTCAATCAGATTATATGGTCTGG
AAAGATGCCGGTCGTCTTTGTGTTATTGATACTGCAAAACACCTTTCTAATATAAAGAAG
TCTGTATTTTCATCCGAAGAAGTTCTCGCTTTTTTAAGAGAACTCCCTCACCGCACATTT
ATCCAGACCGAAGTTCGCGGCCTTGGCGTTAATGTCGATGGAATTGCATTTAATAATGGT
GATATTCCGTCATTAAAAACCTTTTCAAATTGCGTTCAGGTAAAAGTTTCTCGGACTAAT
ACATCCCTAGTTCAAACACTTAATCGTTGGTTTGAAGGAGGAAAAGTTTCTCCTCCGAGC
ATTCAATTTGAACGGGCGTATTATAAAAAAGACGATCAAATTCATGAAGACGCAGCGAAA
AGAAAGATACGATTCCAGATGCCCGCAACTGAGTTGGTTCATGCTTCTGACGATGCGGGG
TGGACACCAAGTTATTTGCTCGGCATTGATCCTGGCGAGTATGGAATGGGTCTTTCATTG
GTTTCGATTAATAACGGAGAAGTCTTAGATTCAGGCTTTATTCATATTAATTCTCTGATC
AATTTTGCCTCTAAAAAGAGCAACCATCAAACTAAGGTTGTTCCGCGTCAGCAGTACAAA
TCTCCTTATGCAAATTATTTAGAACAATCTAAAGATTCTGCTGCTGGTGATATTGCGCAT
ATACTCGATCGACTTATATACAAATTAAATGCGTTGCCTGTTTTTGAGGCTCTTTCAGGT
AATTCTCAGAGTGCTGCTGATCAAGTTTGGACGAAAGTCTTATCGTTTTACACTTGGGGT
GATAATGACGCTCAGAATTCTATTAGAAAGCAGCATTGGTTTGGAGCCAGTCATTGGGAT
ATCAAAGGTATGTTAAGGCAACCCCCTACGGAGAAGAAGCCTAAACCGTATATTGCTTTT
CCTGGCTCTCAGGTTTCTTCGTATGGTAATTCCCAACGTTGCTCTTGCTGCGGTCGCAAT
CCTATTGAACAACTTCGAGAAATGGCAAAGGATACCTCTATTAAAGAGCTAAAAATTCGC
AATTCTGAGATACAGCTTTTTGACGGAACCATTAAATTATTTAATCCAGACCCATCCACT
GTGATAGAGAGAAGGCGACATAATCTTGGTCCATCAAGAATTCCTGTTGCTGACCGTACT
TTCAAAAACATCAGTCCATCAAGTCTAGAATTTAAAGAATTGATTACTATCGTGTCTCGA
TCTATCCGTCATTCACCTGAGTTTATCGCTAAAAAACGCGGCATAGGGTCTGAGTATTTT
TGCGCTTATTCCGATTGCAACTCATCCTTAAATTCTGAAGCTAACGCAGCTGCTAACGTA
GCGCAAAAATTTCAAAAACAGTTATTTTTTGAGTTATAA
SEQ ATGAAGAGAATTCTGAACAGTCTGAAAGTTGCTGCCTTGAGACTTCTGTTTCGAGGCAAA
ID GGTTCTGAATTAGTGAAGACAGTCAAATATCCATTGGTTTCCCCGGTTCAAGGCGCGGTT
NO: GAAGAACTTGCTGAAGCAATTCGGCACGACAACCTGCACCTTTTTGGGCAGAAGGAAATA
37 GTGGATCTTATGGAGAAAGACGAAGGAACCCAGGTGTATTCGGTTGTGGATTTTTGGTTG
GATACCCTGCGTTTAGGGATGTTTTTCTCACCATCAGCGAATGCGTTGAAAATCACGCTG
GGAAAATTCAATTCTGATCAGGTTTCACCTTTTCGTAAGGTTTTGGAGCAGTCACCTTTT
TTTCTTGCGGGTCGCTTGAAGGTTGAACCTGCGGAAAGGATACTTTCTGTTGAAATCAGA
AAGATTGGTAAAAGAGAAAACAGAGTTGAGAACTATGCCGCCGATGTGGAGACATGCTTC
ATTGGTCAGCTTTCTTCAGATGAGAAACAGAGTATCCAGAAGCTGGCAAATGATATCTGG
GATAGCAAGGATCATGAGGAACAGAGAATGTTGAAGGCGGATTTTTTTGCTATACCTCTT
ATAAAAGACCCCAAAGCTGTCACAGAAGAAGATCCTGAAAATGAAACGGCGGGAAAACAG
AAACCGCTTGAATTATGTGTTTGTCTTGTTCCTGAGTTGTATACCCGAGGTTTCGGCTCC
ATTGCTGATTTTCTGGTTCAGCGACTTACCTTGCTGCGTGACAAAATGAGTACCGACACG
GCGGAAGATTGCCTCGAGTATGTTGGCATTGAGGAAGAAAAAGGCAATGGAATGAATTCC
TTGCTCGGCACTTTTTTGAAGAACCTGCAGGGTGATGGTTTTGAACAGATTTTTCAGTTT
ATGCTTGGGTCTTATGTTGGCTGGCAGGGGAAGGAAGATGTACTGCGCGAACGATTGGAT
TTGCTGGCCGAAAAAGTCAAAAGATTACCAAAGCCAAAATTTGCCGGAGAATGGAGTGGT
CATCGTATGTTTCTCCATGGTCAGCTGAAAAGCTGGTCGTCGAATTTCTTCCGTCTTTTT
AATGAGACGCGGGAACTTCTGGAAAGTATCAAGAGTGATATTCAACATGCCACCATGCTC
ATTAGCTATGTGGAAGAGAAAGGAGGCTATCATCCACAGCTGTTGAGTCAGTATCGGAAG
TTAATGGAACAATTACCGGCGTTGCGGACTAAGGTTTTGGATCCTGAGATTGAGATGACG
CATATGTCCGAGGCTGTTCGAAGTTACATTATGATACACAAGTCTGTAGCGGGATTTCTG
CCGGATTTACTCGAGTCTTTGGATCGAGATAAGGATAGGGAATTTTTGCTTTCCATCTTT
CCTCGTATTCCAAAGATAGATAAGAAGACGAAAGAGATCGTTGCATGGGAGCTACCGGGC
GAGCCAGAGGAAGGCTATTTGTTCACAGCAAACAACCTTTTCCGGAATTTTCTTGAGAAT
CCGAAACATGTGCCACGATTTATGGCAGAGAGGATTCCCGAGGATTGGACGCGTTTGCGC
TCGGCCCCTGTGTGGTTTGATGGGATGGTGAAGCAATGGCAGAAGGTGGTGAATCAGTTG
GTTGAATCTCCAGGCGCCCTTTATCAGTTCAATGAAAGTTTTTTGCGTCAAAGACTGCAA
GCAATGCTTACGGTCTATAAGCGGGATCTCCAGACTGAGAAGTTTCTGAAGCTGCTGGCT
GATGTCTGTCGTCCACTCGTTGATTTTTTCGGACTTGGAGGAAATGATATTATCTTCAAG
TCATGTCAGGATCCAAGAAAGCAATGGCAGACTGTTATTCCACTCAGTGTCCCAGCGGAT
GTTTATACAGCATGTGAAGGCTTGGCTATTCGTCTCCGCGAAACTCTTGGATTCGAATGG
AAAAATCTGAAAGGACACGAGCGGGAAGATTTTTTACGGCTGCATCAGTTGCTGGGAAAT
CTGCTGTTCTGGATCAGGGATGCGAAACTTGTCGTGAAGCTGGAAGACTGGATGAACAAT
CCTTGTGTTCAGGAGTATGTGGAAGCACGAAAAGCCATTGATCTTCCCTTGGAGATTTTC
GGATTTGAGGTGCCGATTTTTCTCAATGGCTATCTCTTTTCGGAACTGCGCCAGCTGGAA
TTGTTGCTGAGGCGTAAGTCGGTGATGACGTCTTACAGCGTCAAAACGACAGGCTCGCCA
AATAGGCTCTTCCAGTTGGTTTACCTACCTCTAAACCCTTCAGATCCGGAAAAGAAAAAT
TCCAACAACTTTCAGGAGCGCCTCGATACACCTACCGGTTTGTCGCGTCGTTTTCTGGAT
CTTACGCTGGATGCATTTGCTGGCAAACTCTTGACGGATCCGGTAACTCAGGAACTGAAG
ACGATGGCCGGTTTTTACGATCATCTCTTTGGCTTCAAGTTGCCGTGTAAACTGGCGGCG
ATGAGTAACCATCCAGGATCCTCTTCCAAAATGGTGGTTCTGGCAAAACCAAAGAAGGGT
GTTGCTAGTAACATCGGCTTTGAACCTATTCCCGATCCTGCTCATCCTGTGTTCCGGGTG
AGAAGTTCCTGGCCGGAGTTGAAGTACCTGGAGGGGTTGTTGTATCTTCCCGAAGATACA
CCACTGACCATTGAACTGGCGGAAACGTCGGTCAGTTGTCAGTCTGTGAGTTCAGTCGCT
TTCGATTTGAAGAATCTGACGACTATCTTGGGTCGTGTTGGTGAATTCAGGGTGACGGCA
GATCAACCTTTCAAGCTGACGCCCATTATTCCTGAGAAAGAGGAATCCTTCATCGGGAAG
ACCTACCTCGGTCTTGATGCTGGAGAGCGATCTGGCGTTGGTTTCGCGATTGTGACGGTT
GACGGCGATGGGTATGAGGTGCAGAGGTTGGGTGTGCATGAAGATACTCAGCTTATGGCG
CTTCAGCAAGTCGCCAGCAAGTCTCTTAAGGAGCCGGTTTTCCAGCCACTCCGTAAGGGC
ACATTTCGTCAGCAGGAGCGCATTCGCAAAAGCCTCCGCGGTTGCTACTGGAATTTCTAT
CATGCATTGATGATCAAGTACCGAGCTAAAGTTGTGCATGAGGAATCGGTGGGTTCATCC
GGTCTGGTGGGGCAGTGGCTGCGTGCATTTCAGAAGGATCTCAAAAAGGCTGATGTTCTG
CCCAAGAAGGGTGGAAAAAATGGTGTAGACAAAAAAAAGAGAGAAAGCAGCGCTCAGGAT
ACCTTATGGGGAGGAGCTTTCTCGAAGAAGGAAGAGCAGCAGATAGCCTTTGAGGTTCAG
GCAGCTGGATCAAGCCAGTTTTGTCTGAAGTGTGGTTGGTGGTTTCAGTTGGGGATGCGG
GAAGTAAATCGTGTGCAGGAGAGTGGCGTGGTGCTGGACTGGAACCGGTCCATTGTAACC
TTCCTCATCGAATCCTCAGGAGAAAAGGTATATGGTTTCAGTCCTCAGCAACTGGAAAAA
GGCTTTCGTCCTGACATCGAAACGTTCAAAAAAATGGTAAGGGATTTTATGAGACCCCCC
ATGTTTGATCGCAAAGGTCGGCCGGCCGCGGCGTATGAAAGATTCGTACTGGGACGTCGT
CACCGTCGTTATCGCTTTGATAAAGTTTTTGAAGAGAGATTTGGTCGCAGTGCTCTTTTC
ATCTGCCCGCGGGTCGGGTGTGGGAATTTCGATCACTCCAGTGAGCAGTCAGCCGTTGTC
CTTGCCCTTATTGGTTACATTGCTGATAAGGAAGGGATGAGTGGTAAGAAGCTTGTTTAT
GTGAGGCTGGCTGAACTTATGGCTGAGTGGAAGCTGAAGAAACTGGAGAGATCAAGGGTG
GAAGAACAGAGCTCGGCACAATAA
SEQ ATGGCAGAAAGCAAGCAGATGCAATGCCGCAAGTGCGGCGCAAGCATGAAGTATGAAGTA
ID ATTGGATTGGGCAAGAAGTCATGCAGATATATGTGCCCAGATTGCGGCAATCACACCAGC
NO: GCGCGCAAGATTCAGAACAAGAAAAAGCGCGACAAAAAGTATGGATCCGCAAGCAAAGCG
38 CAGAGCCAGAGGATAGCTGTGGCTGGCGCGCTTTATCCAGACAAAAAAGTGCAGACCATA
AAGACCTACAAATACCCAGCGGATCTTAATGGCGAAGTTCATGACAGCGGCGTCGCAGAG
AAGATTGCGCAGGCGATTCAGGAAGATGAGATCGGCCTGCTTGGCCCGTCCAGCGAATAC
GCTTGCTGGATTGCTTCACAAAAACAGAGCGAGCCGTATTCAGTTGTAGATTTTTGGTTT
GACGCGGTGTGCGCAGGCGGAGTATTCGCGTATTCTGGCGCGCGCCTGCTTTCCACAGTC
CTCCAGTTGAGTGGCGAGGAAAGCGTTTTGCGCGCTGCTTTAGCATCTAGCCCGTTTGTA
GATGACATTAATTTGGCGCAAGCGGAAAAGTTCCTAGCCGTTAGCCGGCGCACAGGCCAA
GATAAGCTAGGCAAGCGCATTGGAGAATGTTTTGCGGAAGGCCGGCTTGAAGCGCTTGGC
ATCAAAGATCGCATGCGCGAATTCGTGCAAGCGATTGATGTGGCCCAAACCGCGGGCCAG
CGGTTCGCGGCCAAGCTAAAGATATTCGGCATCAGTCAGATGCCTGAAGCCAAGCAATGG
AACAATGATTCCGGGCTCACTGTATGTATTTTGCCGGATTATTATGTCCCGGAAGAAAAC
CGCGCGGACCAGCTGGTTGTTTTGCTTCGGCGCTTACGCGAGATCGCGTATTGCATGGGA
ATTGAGGATGAAGCAGGATTTGAGCATCTAGGCATTGACCCTGGTGCTCTTTCCAATTTT
TCCAATGGCAATCCAAAGCGAGGATTTCTCGGCCGCCTGCTCAATAATGACATTATAGCG
CTGGCAAACAACATGTCAGCCATGACGCCGTATTGGGAAGGCAGAAAAGGCGAGTTGATT
GAGCGCCTTGCATGGCTTAAACATCGCGCTGAAGGATTGTATTTGAAAGAGCCACATTTC
GGCAACTCCTGGGCAGACCACCGCAGCAGGATTTTCAGTCGCATTGCGGGCTGGCTTTCC
GGATGCGCGGGCAAGCTCAAGATTGCCAAGGATCAGATTTCAGGCGTGCGTACGGATTTG
TTTCTGCTCAAGCGCCTTCTGGATGCGGTACCGCAAAGCGCGCCGTCGCCGGACTTTATT
GCTTCCATCAGCGCGCTGGATCGGTTTTTGGAAGCGGCAGAAAGCAGCCAGGATCCGGCA
GAACAGGTACGCGCTTTGTACGCGTTTCATCTGAACGCGCCTGCGGTCCGATCCATCGCC
AACAAGGCGGTACAGAGGTCTGATTCCCAGGAGTGGCTTATCAAGGAACTGGATGCTGTA
GATCACCTTGAATTCAACAAAGCATTTCCGTTTTTTTCGGATACAGGAAAGAAAAAGAAG
AAAGGAGCGAATAGCAACGGAGCGCCTTCTGAAGAAGAATACACGGAAACAGAATCCATT
CAACAACCAGAAGATGCAGAGCAGGAAGTGAATGGTCAAGAAGGAAATGGCGCTTCAAAG
AACCAGAAAAAGTTTCAGCGCATTCCTCGATTTTTCGGGGAAGGGTCAAGGAGTGAGTAT
CGAATTTTAACAGAAGCGCCGCAATATTTTGACATGTTCTGCAATAATATGCGCGCGATC
TTTATGCAGCTAGAGAGTCAGCCGCGCAAGGCGCCTCGTGATTTCAAATGCTTTCTGCAG
AATCGTTTGCAGAAGCTTTACAAGCAAACCTTTCTCAATGCTCGCAGTAATAAATGCCGC
GCGCTTCTGGAATCCGTCCTTATTTCATGGGGAGAATTTTATACTTATGGCGCGAATGAA
AAGAAGTTTCGTCTGCGCCATGAAGCGAGCGAGCGCAGCTCGGATCCGGACTATGTGGTT
CAGCAGGCATTGGAAATCGCGCGCCGGCTTTTCTTGTTCGGATTTGAGTGGCGCGATTGC
TCTGCTGGAGAGCGCGTGGATTTGGTTGAAATCCACAAAAAAGCAATCTCATTTTTGCTT
GCAATCACTCAGGCCGAGGTTTCAGTTGGTTCCTATAACTGGCTTGGGAATAGCACCGTG
AGCCGGTATCTTTCGGTTGCTGGCACAGACACATTGTACGGCACTCAACTGGAGGAGTTT
TTGAACGCCACAGTGCTTTCACAGATGCGTGGGCTGGCGATTCGGCTTTCATCTCAGGAG
TTAAAAGACGGATTTGATGTTCAGTTGGAGAGTTCGTGCCAGGACAATCTCCAGCATCTG
CTGGTGTATCGCGCTTCGCGCGACTTGGCTGCGTGCAAACGCGCTACATGCCCGGCTGAA
TTGGATCCGAAAATTCTTGTTCTGCCGGTTGGTGCGTTTATCGCGAGCGTAATGAAAATG
ATTGAGCGTGGCGATGAACCATTAGCAGGCGCGTATTTGCGTCATCGGCCGCATTCATTC
GGCTGGCAGATACGGGTTCGTGGAGTGGCGGAAGTAGGCATGGATCAGGGCACAGCGCTA
GCATTCCAGAAGCCGACTGAATCAGAGCCGTTTAAAATAAAGCCGTTTTCCGCTCAATAC
GGCCCAGTACTTTGGCTTAATTCTTCATCCTATAGCCAGAGCCAGTATCTGGATGGATTT
TTAAGCCAGCCAAAGAATTGGTCTATGCGGGTGCTACCTCAAGCCGGATCAGTGCGCGTG
GAACAGCGCGTTGCTCTGATATGGAATTTGCAGGCAGGCAAGATGCGGCTGGAGCGCTCT
GGAGCGCGCGCGTTTTTCATGCCAGTGCCATTCAGCTTCAGGCCGTCTGGTTCAGGAGAT
GAAGCAGTATTGGCGCCGAATCGGTACTTGGGACTTTTTCCGCATTCCGGAGGAATAGAA
TACGCGGTGGTGGATGTATTAGATTCCGCGGGTTTCAAAATTCTTGAGCGCGGTACGATT
GCGGTAAATGGCTTTTCCCAGAAGCGCGGCGAACGCCAAGAGGAGGCACACAGAGAAAAA
CAGAGACGCGGAATTTCTGATATAGGCCGCAAGAAGCCGGTGCAAGCTGAAGTTGACGCA
GCCAATGAATTGCACCGCAAATACACCGATGTTGCCACTCGTTTAGGGTGCAGAATTGTG
GTTCAGTGGGCGCCCCAGCCAAAGCCGGGCACAGCGCCGACCGCGCAAACAGTATACGCG
CGCGCAGTGCGGACCGAAGCGCCGCGATCTGGAAATCAAGAGGATCATGCTCGTATGAAA
TCCTCTTGGGGATATACCTGGGGCACCTATTGGGAGAAGCGCAAACCAGAGGATATTTTG
GGCATCTCAACCCAAGTATACTGGACCGGCGGTATAGGCGAGTCATGTCCCGCAGTCGCG
GTTGCGCTTTTGGGGCACATTAGGGCAACATCCACTCAAACTGAATGGGAAAAAGAGGAG
GTTGTATTCGGTCGACTGAAGAAGTTCTTTCCAAGCTAG
SEQ ATGGAAAAGAGAATAAACAAGATACGAAAGAAACTATCGGCCGATAATGCCACAAAGCCT
ID GTGAGCAGGAGCGGCCCCATGAAAACACTCCTTGTCCGGGTCATGACGGACGACTTGAAA
NO: AAAAGACTGGAGAAGCGTCGGAAAAAGCCGGAAGTTATGCCGCAGGTTATTTCAAATAAC
39 GCAGCAAACAATCTTAGAATGCTCCTTGATGACTATACAAAGATGAAGGAGGCGATACTA
CAAGTTTACTGGCAGGAATTTAAGGACGACCATGTGGGCTTGATGTGCAAATTTGCCCAG
CCTGCTTCCAAAAAAATTGACCAGAACAAACTAAAACCGGAAATGGATGAAAAAGGAAAT
CTAACAACTGCCGGTTTTGCATGTTCTCAATGCGGTCAGCCGCTATTTGTTTATAAGCTT
GAACAGGTGAGTGAAAAAGGCAAGGCTTATACAAATTACTTCGGCCGGTGTAATGTGGCC
GAGCATGAGAAATTGATTCTTCTTGCTCAATTAAAACCTGAAAAAGACAGTGACGAAGCA
GTGACATACTCCCTTGGCAAATTCGGCCAGAGGGCATTGGACTTTTATTCAATCCACGTA
ACAAAAGAATCCACCCATCCAGTAAAGCCCCTGGCACAGATTGCGGGCAACCGCTATGCA
AGCGGACCTGTTGGCAAGGCCCTTTCCGATGCCTGTATGGGCACTATAGCCAGTTTTCTT
TCGAAATATCAAGACATCATCATAGAACATCAAAAGGTTGTGAAGGGTAATCAAAAGAGG
TTAGAGAGTCTCAGGGAATTGGCAGGGAAAGAAAATCTTGAGTACCCATCGGTTACACTG
CCGCCGCAGCCGCATACGAAAGAAGGGGTTGACGCTTATAACGAAGTTATTGCAAGGGTA
CGTATGTGGGTTAATCTTAATCTGTGGCAAAAGCTGAAGCTCAGCCGTGATGACGCAAAA
CCGCTACTGCGGCTAAAAGGATTCCCATCTTTCCCTGTTGTGGAGCGGCGTGAAAACGAA
GTTGACTGGTGGAATACGATTAATGAAGTAAAAAAACTGATTGACGCTAAACGAGATATG
GGACGGGTATTCTGGAGCGGCGTTACCGCAGAAAAGAGAAATACCATCCTTGAAGGATAC
AACTATCTGCCAAATGAGAATGACCATAAAAAGAGAGAGGGCAGTTTGGAAAACCCTAAG
AAGCCTGCCAAACGCCAGTTTGGAGACCTCTTGCTGTATCTTGAAAAGAAATATGCCGGA
GACTGGGGAAAGGTCTTCGATGAGGCATGGGAGAGGATAGATAAGAAAATAGCCGGACTC
ACAAGCCATATAGAGCGCGAAGAAGCAAGAAACGCGGAAGACGCTCAATCCAAAGCCGTA
CTTACAGACTGGCTAAGGGCAAAGGCATCATTTGTTCTTGAAAGACTGAAGGAAATGGAT
GAAAAGGAATTCTATGCGTGTGAAATCCAACTTCAAAAATGGTATGGCGATCTTCGAGGC
AACCCGTTTGCCGTTGAAGCTGAGAATAGAGTTGTTGATATAAGCGGGTTTTCTATCGGA
AGCGATGGCCATTCAATCCAATACAGAAATCTCCTTGCCTGGAAATATCTGGAGAACGGC
AAGCGTGAATTCTATCTGTTAATGAATTATGGCAAGAAAGGGCGCATCAGATTTACAGAT
GGAACAGATATTAAAAAGAGCGGCAAATGGCAGGGACTATTATATGGCGGTGGCAAGGCA
AAGGTTATTGATCTGACTTTCGACCCCGATGATGAACAGTTGATAATCCTGCCGCTGGCC
TTTGGCACAAGGCAAGGCCGCGAGTTTATCTGGAACGATTTGCTGAGTCTTGAAACAGGC
CTGATAAAGCTCGCAAACGGAAGAGTTATCGAAAAAACAATCTATAACAAAAAAATAGGG
CGGGATGAACCGGCTCTATTCGTTGCCTTAACATTTGAGCGCCGGGAAGTTGTTGATCCA
TCAAATATAAAGCCTGTAAACCTTATAGGCGTTGACCGCGGCGAAAACATCCCGGCGGTT
ATTGCATTGACAGACCCTGAAGGTTGTCCTTTACCGGAATTCAAGGATTCATCAGGGGGC
CCAACAGACATCCTGCGAATAGGAGAAGGATATAAGGAAAAGCAGAGGGCTATTCAGGCA
GCAAAGGAGGTAGAGCAAAGGCGGGCTGGCGGTTATTCACGGAAGTTTGCATCCAAGTCG
AGGAACCTGGCGGACGACATGGTGAGAAATTCAGCGCGAGACCTTTTTTACCATGCCGTT
ACCCACGATGCCGTCCTTGTCTTTGAAAACCTGAGCAGGGGTTTTGGAAGGCAGGGCAAA
AGGACCTTCATGACGGAAAGACAATATACAAAGATGGAAGACTGGCTGACAGCGAAGCTC
GCATACGAAGGTCTTACGTCAAAAACCTACCTTTCAAAGACGCTGGCGCAATATACGTCA
AAAACATGCTCCAACTGCGGGTTTACTATAACGACTGCCGATTATGACGGGATGTTGGTA
AGGCTTAAAAAGACTTCTGATGGATGGGCAACTACCCTCAACAACAAAGAATTAAAAGCC
GAAGGCCAGATAACGTATTATAACCGGTATAAAAGGCAAACCGTGGAAAAAGAACTCTCC
GCAGAGCTTGACAGGCTTTCAGAAGAGTCGGGCAATAATGATATTTCTAAGTGGACCAAG
GGTCGCCGGGACGAGGCATTATTTTTGTTAAAGAAAAGATTCAGCCATCGGCCTGTTCAG
GAACAGTTTGTTTGCCTCGATTGCGGCCATGAAGTCCACGCCGATGAACAGGCAGCCTTG
AATATTGCAAGGTCATGGCTTTTTCTAAACTCAAATTCAACAGAATTCAAAAGTTATAAA
TCGGGTAAACAGCCCTTCGTTGGTGCTTGGCAGGCCTTTTACAAAAGGAGGCTTAAAGAG
GTATGGAAGCCCAACGCC
SEQ ATGAAAAGGATAAATAAAATACGAAGGAGATTGGTAAAGGATAGCAACACGAAAAAAGCC
ID GGCAAAACCGGCCCTATGAAAACCTTGCTCGTTCGGGTTATGACACCTGACCTGAGAGAA
NO: AGGTTAGAGAATCTTCGCAAAAAGCCGGAAAACATTCCTCAGCCCATTTCAAATACTTCA
40 CGTGCAAATTTAAATAAACTCCTCACTGACTATACGGAAATGAAGAAAGCAATCCTGCAT
GTTTATTGGGAAGAGTTCCAAAAAGACCCTGTCGGATTGATGAGCAGGGTTGCACAACCA
GCGCCCAAGAATATTGATCAGAGAAAATTGATTCCGGTGAAGGACGGAAATGAGAGACTA
ACAAGTTCTGGATTTGCCTGTTCTCAGTGCTGTCAACCCCTCTATGTTTATAAGCTTGAA
CAAGTGAATGACAAGGGTAAGCCCCATACAAATTACTTTGGCCGTTGTAATGTCTCCGAG
CATGAACGTTTGATATTGCTCTCGCCGCATAAACCGGAGGCAAATGACGAGCTAGTAACG
TATTCGTTGGGGAAGTTCGGTCAAAGGGCATTGGACTTTTATTCAATCCACGTAACAAGA
GAATCGAACCATCCTGTAAAGCCGCTAGAACAGATCGGTGGCAATAGCTGCGCAAGTGGT
CCCGTTGGTAAGGCTTTATCTGATGCCTGTATGGGAGCAGTAGCCAGTTTCCTTACAAAG
TACCAGGACATCATCCTCGAACACCAAAAGGTTATAAAAAAAAACGAAAAGAGATTGGCA
AATCTAAAGGATATAGCAAGTGCAAACGGGCTTGCATTTCCTAAAATCACTCTTCCACCG
CAACCGCATACAAAAGAAGGGATTGAAGCTTATAACAATGTTGTTGCTCAGATAGTGATC
TGGGTAAACCTGAATCTTTGGCAGAAACTCAAAATTGGCAGGGATGAGGCAAAGCCCTTA
CAGCGGCTTAAGGGTTTTCCGTCCTTCCCTCTTGTTGAACGCCAGGCGAATGAGGTTGAT
TGGTGGGATATGGTCTGTAATGTCAAAAAGTTGATTAACGAAAAGAAAGAGGACGGGAAG
GTCTTCTGGCAAAATCTTGCTGGATATAAAAGGCAGGAAGCCTTGCTTCCATATCTTTCG
TCTGAAGAAGACCGTAAAAAAGGAAAAAAGTTTGCGCGTTATCAGTTTGGTGACCTTTTG
CTTCACCTTGAAAAGAAACACGGTGAAGATTGGGGCAAAGTTTATGATGAGGCATGGGAA
AGAATAGATAAAAAAGTTGAAGGTCTGAGTAAGCACATAAAGTTGGAGGAAGAAAGAAGG
TCTGAAGATGCTCAATCAAAGGCTGCCCTCACTGATTGGCTCAGGGCAAAGGCCTCTTTT
GTTATTGAAGGGCTCAAAGAAGCTGATAAGGATGAGTTTTGCAGGTGTGAGTTAAAGCTT
CAAAAGTGGTATGGAGATTTGAGAGGAAAACCATTTGCTATAGAAGCAGAGAACAGCATT
TTAGATATAAGCGGATTTTCTAAACAGTATAATTGTGCATTTATATGGCAGAAAGACGGC
GTAAAGAAGTTAAATCTTTATTTAATAATAAATTACTTCAAAGGTGGTAAGCTACGCTTC
AAAAAAATCAAGCCAGAAGCTTTTGAAGCAAATAGGTTTTATACAGTAATTAATAAAAAA
AGCGGTGAGATTGTGCCTATGGAGGTCAACTTCAATTTTGATGACCCGAATTTGATAATT
CTGCCTTTGGCCTTTGGAAAAAGGCAGGGGAGGGAGTTTATCTGGAACGACCTATTGAGC
CTTGAGACGGGTTCATTGAAACTCGCCAATGGCAGGGTTATTGAAAAAACGCTCTATAAC
AGAAGGACGAGACAGGATGAACCAGCACTTTTTGTTGCCCTGACATTTGAAAGAAGAGAG
GTGCTTGACTCATCGAATATAAAACCGATGAATCTGATAGGAATAGACCGGGGAGAAAAT
ATCCCGGCAGTCATAGCATTAACAGACCCGGAAGGATGCCCCTTGTCAAGATTCAAAGAT
TCATTGGGCAATCCAACGCATATTTTGCGAATAGGAGAAAGTTATAAGGAAAAACAACGG
ACTATTCAGGCTGCTAAAGAAGTTGAACAAAGGCGGGCAGGCGGATATTCGAGAAAATAT
GCATCAAAGGCGAAGAATCTGGCGGACGATATGGTAAGAAATACAGCTCGTGACCTCTTA
TATTATGCTGTTACTCAAGATGCAATGCTCATTTTTGAAAATCTTTCCCGCGGTTTTGGT
AGACAAGGCAAGAGGACTTTTATGGCGGAAAGGCAGTACACGAGGATGGAAGACTGGCTG
ACTGCAAAGCTTGCCTATGAAGGTCTGCCATCAAAAACCTATCTTTCAAAGACTCTGGCA
CAGTATACCTCAAAGACATGTTCTAATTGTGGTTTTACAATCACAAGTGCAGATTATGAC
AGGGTGCTCGAAAAGCTCAAGAAGACGGCTACTGGATGGATGACTACAATCAATGGAAAA
GAGTTAAAAGTTGAAGGACAGATAACATACTATAACCGGTATAAAAGGCAGAATGTGGTA
AAAGACCTCTCTGTAGAGCTGGATAGACTTTCGGAAGAGTCGGTAAATAATGATATTTCT
AGTTGGACAAAAGGCCGCAGTGGTGAAGCTTTATCTCTGCTAAAAAAGAGATTTAGTCAC
AGGCCGGTGCAGGAAAAGTTTGTTTGCCTGAACTGTGGTTTTGAAACCCATGCAGACGAA
CAAGCAGCACTGAATATTGCAAGGTCGTGGCTCTTTCTCCGTTCTCAAGAATATAAGAAG
TATCAAACCAATAAAACGACCGGAAATACTGACAAAAGGGCATTTGTTGAAACATGGCAA
TCCTTTTACAGAAAGAAGCTCAAAGAAGTATGGAAACCA
SEQ ATGGGTAAAATGTATTACCTTGGTTTAGACATTGGCACGAATTCCGTGGGCTACGCGGTG
ID ACCGACCCCTCATACCACCTGCTGAAGTTTAAGGGGGAACCAATGTGGGGTGCGCACGTA
NO: TTTGCCGCCGGTAATCAGAGCGCGGAACGACGCTCGTTCCGCACATCGCGTCGTCGTTTG
41 GACCGACGCCAACAGCGCGTTAAACTGGTACAGGAGATTTTTGCCCCGGTGATTAGTCCG
ATCGACCCACGCTTCTTCATTCGTCTGCATGAATCCGCCCTGTGGCGCGATGACGTCGCG
GAGACGGATAAACATATCTTTTTCAATGATCCTACCTATACCGATAAGGAATATTATAGC
GATTACCCGACTATCCATCACCTGATCGTTGATCTGATGGAAAGCTCTGAGAAACACGAT
CCGCGGCTGGTGTACCTTGCAGTGGCGTGGTTAGTGGCACACCGTGGTCATTTTCTGAAC
GAGGTGGACAAGGATAATATTGGAGATGTGTTGTCGTTCGACGCATTTTATCCGGAGTTT
CTCGCGTTCCTGTCGGACAACGGTGTATCACCGTGGGTGTGCGAAAGCAAAGCGCTGCAG
GCGACCTTGCTGAGCCGTAACTCAGTGAACGACAAATATAAAGCCCTTAAGTCTCTGATC
TTCGGATCCCAGAAACCTGAAGATAACTTCGATGCCAATATTTCGGAAGATGGACTCATT
CAACTGCTGGCCGGCAAAAAGGTAAAAGTTAACAAACTGTTCCCTCAGGAATCGAACGAT
GCATCCTTCACATTGAATGATAAAGAAGACGCGATAGAAGAAATCCTGGGTACGCTTACA
CCAGATGAATGTGAATGGATTGCGCATATACGCCGCCTTTTTGACTGGGCTATCATGAAA
CATGCTCTGAAAGATGGCAGGACTATTAGCGAGTCAAAAGTCAAACTGTATGAGCAGCAC
CATCACGATCTGACCCAACTTAAATACTTCGTGAAAACCTACCTTGCAAAAGAATACGAC
GATATTTTCCGCAACGTGGATAGCGAAACAACGAAAAACTATGTAGCGTATTCCTATCAT
GTGAAAGAGGTGAAAGGCACTCTGCCTAAAAATAAGGCAACGCAAGAAGAGTTTTGTAAG
TATGTCCTGGGCAAGGTTAAAAACATTGAATGCTCTGAAGCAGACAAGGTTGACTTTGAT
GAGATGATTCAGCGTCTTACCGACAACTCTTTTATGCCTAAGCAGGTTTCGGGCGAAAAC
CGCGTTATTCCTTATCAGTTATATTATTATGAACTGAAGACAATTCTGAATAAAGCAGCC
TCGTACCTGCCTTTCCTGACGCAGTGTGGAAAAGATGCAATTTCGAACCAGGACAAACTA
CTGTCGATCATGACGTTCCGTATTCCTTACTTCGTCGGACCCTTGCGAAAAGATAATTCG
GAACATGCATGGCTCGAACGAAAGGCCGGTAAGATTTATCCGTGGAACTTTAACGACAAA
GTGGACTTGGATAAATCAGAAGAAGCGTTCATTCGCCGAATGACCAATACCTGTACCTAT
TATCCCGGCGAAGATGTTTTACCGTTGGATTCGCTGATCTATGAGAAATTTATGATTTTA
AATGAAATCAATAATATTCGTATTGACGGCTACCCGATTAGTGTTGACGTTAAACAGCAG
GTTTTTGGCTTGTTCGAAAAAAAACGACGCGTAACCGTGAAAGATATTCAGAACCTGCTG
CTGTCTCTCGGAGCTCTGGACAAACACGGGAAGCTGACAGGCATCGATACCACTATCCAC
TCAAACTATAATACGTATCACCATTTTAAATCTCTCATGGAACGCGGCGTCCTGACCCGG
GATGACGTGGAACGCATCGTTGAAAGGATGACCTACAGCGACGATACTAAGCGTGTGCGT
CTGTGGCTGAATAACAACTATGGTACTTTAACCGCCGACGATGTGAAACACATTTCGCGT
CTGCGCAAACACGATTTTGGCCGTTTATCCAAAATGTTCTTAACAGGTCTGAAGGGTGTC
CATAAGGAGACCGGTGAACGTGCCTCCATACTGGATTTCATGTGGAACACGAACGATAAC
CTGATGCAGCTCCTTTCCGAATGCTACACGTTCAGTGATGAAATCACAAAGCTGCAAGAG
GCGTATTATGCAAAAGCCCAGTTGTCTTTAAACGATTTTTTAGACTCGATGTACATCTCT
AACGCGGTGAAACGTCCGATTTACAGAACTCTGGCAGTGGTGAACGATATTCGAAAAGCA
TGTGGGACGGCCCCTAAACGCATTTTCATCGAAATGGCTCGTGATGGTGAATCAAAAAAA
AAGAGAAGTGTTACACGTCGCGAGCAGATCAAAAACCTGTACCGCTCGATTCGTAAAGAT
TTCCAGCAGGAAGTTGATTTTCTGGAAAAGATCCTGGAAAATAAATCTGATGGTCAACTT
CAGTCAGATGCTTTGTATCTTTACTTTGCACAATTAGGGCGCGATATGTACACGGGCGAT
CCAATAAAGCTGGAGCACATCAAAGATCAGAGTTTCTATAACATAGACCATATTTACCCG
CAGTCTATGGTGAAAGACGATTCCCTAGATAACAAAGTGCTGGTGCAAAGCGAAATTAAC
GGCGAGAAAAGCTCGCGATACCCTTTGGACGCCGCGATCCGCAATAAAATGAAGCCCCTT
TGGGACGCTTACTATAATCATGGCCTGATCTCCTTAAAGAAATACCAGCGTCTAACGCGC
TCGACCCCGTTTACCGATGATGAAAAATGGGACTTTATTAATCGCCAGTTAGTGGAAACC
CGTCAATCTACCAAAGCGCTGGCCATTTTGTTGAAGCGTAAGTTTCCAGACACCGAAATT
GTGTATTCGAAGGCGGGGTTATCGTCCGACTTCAGACATGAATTCGGCCTTGTAAAAAGT
CGCAATATTAATGATTTGCACCACGCTAAAGACGCATTCTTGGCTATCGTTACCGGCAAT
GTGTACCATGAAAGATTCAATCGCAGATGGTTTATGGTGAACCAGCCGTACTCAGTTAAA
ACTAAAACTCTTTTTACCCACAGCATAAAGAATGGCAACTTCGTTGCCTGGAACGGCGAA
GAAGATCTCGGTCGTATTGTAAAAATGCTGAAGCAAAACAAAAATACCATTCACTTCACG
CGCTTCTCCTTCGATCGCAAAGAAGGATTATTTGATATCCAACCTCTGAAAGCCAGCACC
GGCTTAGTCCCACGAAAAGCCGGTCTGGATGTCGTTAAATACGGCGGATATGACAAATCT
ACCGCGGCCTATTACCTGCTGGTGAGGTTCACGCTCGAGGACAAGAAAACCCAGCACAAG
CTGATGATGATTCCTGTAGAAGGCCTGTACAAGGCTCGCATTGATCATGACAAGGAATTT
CTTACCGATTATGCGCAAACGACTATAAGCGAAATCCTACAGAAAGATAAACAGAAAGTG
ATCAATATTATGTTTCCAATGGGTACGAGGCATATAAAACTCAATTCAATGATTAGTATC
GATGGCTTCTATCTTAGTATCGGCGGAAAGTCCTCTAAAGGTAAGTCAGTTCTATGTCAC
GCAATGGTTCCACTGATCGTCCCTCACAAAATCGAATGTTACATTAAAGCAATGGAAAGC
TTCGCCCGGAAGTTTAAAGAAAACAACAAGCTGCGCATCGTAGAAAAATTCGATAAAATC
ACCGTTGAAGACAACCTGAATCTCTACGAGCTCTTTCTCCAAAAACTGCAGCATAATCCC
TATAATAAGTTTTTTTCGACACAGTTTGACGTACTGACGAACGGCCGTTCTACTTTCACA
AAACTGTCGCCGGAGGAACAGGTACAGACGCTCTTGAACATTTTAAGTATCTTTAAAACA
TGCCGCAGTTCGGGTTGCGACCTGAAATCCATCAACGGCAGTGCCCAGGCAGCGCGCATC
ATGATTAGCGCTGACTTAACTGGACTGTCGAAAAAATATTCAGATATTAGGTTGGTTGAA
CAGTCAGCTTCTGGTTTGTTCGTATCCAAAAGTCAGAACTTACTGGAGTATCTCTAA
SEQ ATGTCATCGCTCACGAAATTCACTAACAAATACTCTAAACAGCTCACCATTAAGAATGAA
ID CTCATCCCAGTTGGCAAAACACTGGAGAACATCAAAGAGAATGGTCTGATAGATGGCGAC
NO: GAACAGCTGAATGAGAATTATCAGAAGGCGAAAATTATTGTGGATGATTTTCTGCGGGAC
42 TTCATTAATAAAGCACTGAATAATACGCAGATCGGGAACTGGCGCGAACTGGCGGATGCC
CTTAATAAAGAGGATGAAGATAACATCGAGAAATTGCAGGATAAAATTCGGGGAATCATT
GTATCCAAATTTGAAACGTTTGATCTGTTTAGCAGCTATTCTATTAAGAAAGATGAAAAG
ATTATTGACGACGACAATGATGTTGAAGAAGAGGAACTGGATCTGGGCAAGAAGACCAGC
TCATTTAAATACATATTTAAAAAAAACCTGTTTAAGTTAGTGTTGCCATCCTACCTGAAA
ACCACAAACCAGGACAAGCTGAAGATTATTAGCTCGTTTGATAATTTTTCAACGTACTTC
CGCGGGTTCTTTGAAAACCGGAAAAACATTTTTACCAAGAAACCGATCTCCACAAGTATT
GCGTATCGCATTGTTCATGATAACTTCCCGAAATTCCTTGATAACATTCGTTGTTTTAAT
GTGTGGCAGACGGAATGCCCGCAACTAATCGTGAAAGCAGATAACTATCTGAAAAGCAAA
AATGTTATAGCGAAAGATAAAAGTTTGGCAAACTATTTTACCGTGGGCGCGTATGACTAT
TTCCTGTCTCAGAATGGTATAGATTTTTACAACAATATTATAGGTGGACTGCCAGCGTTC
GCCGGCCATGAGAAAATCCAAGGTCTCAATGAATTCATCAATCAAGAGTGCCAAAAAGAC
AGCGAGCTGAAAAGTAAGCTGAAAAACCGTCACGCGTTCAAAATGGCGGTACTGTTCAAA
CAGATACTCAGCGATCGTGAAAAAAGTTTTGTAATTGATGAGTTCGAGTCGGATGCTCAA
GTTATTGACGCCGTTAAAAACTTTTACGCCGAACAGTGCAAAGATAACAATGTTATTTTT
AACTTATTAAATCTTATCAAGAATATCGCTTTCTTAAGTGATGACGAACTGGACGGCATA
TTCATTGAAGGGAAATACCTGTCGAGCGTTAGTCAAAAACTCTATAGCGATTGGTCAAAA
TTACGTAACGACATTGAGGATTCGGCTAACTCTAAACAAGGCAATAAAGAGCTGGCCAAG
AAGATCAAAACCAACAAAGGGGATGTAGAAAAAGCGATCTCGAAATATGAGTTCTCGCTG
TCGGAACTGAACTCGATTGTACATGATAACACCAAGTTTTCTGACCTCCTTAGTTGTACA
CTGCATAAGGTGGCTTCTGAGAAACTGGTGAAGGTCAATGAAGGCGACTGGCCGAAACAT
CTCAAGAATAATGAAGAGAAACAAAAAATCAAAGAGCCGCTTGATGCTCTGCTGGAGATC
TATAATACACTTCTGATTTTTAACTGCAAAAGCTTCAATAAAAACGGCAACTTCTATGTC
GACTATGATCGTTGCATCAATGAACTGAGTTCGGTCGTGTATCTGTATAATAAAACACGT
AACTATTGCACTAAAAAACCCTATAACACGGACAAGTTCAAACTCAATTTTAACAGTCCG
CAGCTCGGTGAAGGCTTTTCCAAGTCGAAAGAAAATGACTGTCTGACTCTTTTGTTTAAA
AAAGACGACAACTATTATGTAGGCATTATCCGCAAAGGTGCAAAAATCAATTTTGATGAT
ACACAAGCAATCGCCGATAACACCGACAATTGCATCTTTAAAATGAATTATTTCCTACTT
AAAGACGCAAAAAAATTTATCCCGAAATGTAGCATTCAGCTGAAAGAAGTCAAGGCCCAT
TTTAAGAAATCTGAAGATGATTACATTTTGTCTGATAAAGAGAAATTTGCTAGCCCGCTG
GTCATTAAAAAGAGCACATTTTTGCTGGCAACTGCACATGTGAAAGGGAAAAAAGGCAAT
ATCAAGAAATTTCAGAAAGAATATTCGAAAGAAAACCCCACTGAGTATCGCAATTCTTTA
AACGAATGGATTGCTTTTTGTAAAGAGTTCTTAAAAACTTATAAAGCGGCTACCATTTTT
GATATAACCACATTGAAAAAGGCAGAGGAATATGCTGATATTGTAGAATTCTACAAGGAT
GTCGATAATCTGTGCTACAAACTGGAGTTCTGCCCGATTAAAACCTCGTTTATAGAAAAC
CTGATAGATAACGGCGACCTGTATCTGTTTCGCATCAATAACAAAGACTTCAGCAGTAAA
TCGACCGGCACCAAGAACCTTCATACGTTATATTTACAAGCTATATTCGATGAACGTAAT
CTGAACAATCCGACAATTATGCTGAATGGGGGAGCAGAACTGTTCTATCGTAAAGAAAGT
ATTGAGCAGAAAAACCGTATCACACACAAAGCCGGTTCAATTCTCGTGAATAAGGTGTGT
AAAGACGGTACAAGCCTGGATGATAAGATACGTAATGAAATTTATCAATATGAGAATAAA
TTTATTGATACCCTGTCTGATGAAGCTAAAAAGGTGTTACCGAATGTCATTAAAAAGGAA
GCTACCCATGACATTACAAAAGATAAACGTTTCACTAGTGACAAATTCTTCTTTCACTGC
CCCCTGACAATTAATTATAAGGAAGGCGATACCAAGCAGTTCAATAACGAAGTGCTGAGT
TTTCTGCGTGGAAATCCTGACATCAACATTATCGGCATTGACCGCGGAGAGCGTAATTTA
ATCTATGTAACGGTTATAAACCAGAAAGGCGAGATTCTGGATTCGGTTTCATTCAATACC
GTGACCAACAAGAGTTCAAAAATCGAGCAGACAGTCGATTATGAAGAGAAATTGGCAGTC
CGCGAGAAAGAGAGGATTGAAGCAAAACGTTCCTGGGACTCTATCTCAAAAATTGCGACA
CTAAAGGAAGGTTATCTGAGCGCAATAGTTCACGAGATCTGTCTGTTAATGATTAAACAC
AACGCGATCGTTGTCTTAGAGAATCTTAATGCAGGCTTTAAGCGTATTCGTGGCGGTTTA
TCAGAAAAAAGTGTTTATCAAAAATTCGAAAAAATGTTGATTAACAAACTGAACTATTTT
GTCAGCAAGAAGGAATCCGACTGGAATAAACCGTCTGGTCTGCTGAATGGACTGCAGCTT
TCGGATCAGTTTGAAAGCTTCGAAAAACTGGGTATTCAGTCTGGTTTTATTTTTTACGTG
CCGGCTGCATATACCTCAAAGATTGATCCGACCACGGGCTTCGCCAATGTTCTGAATCTG
TCGAAGGTACGCAATGTTGATGCGATCAAAAGCTTTTTTTCTAACTTCAACGAAATTAGT
TATAGCAAGAAAGAAGCCCTTTTCAAATTCTCATTCGATCTGGATTCACTGAGTAAGAAA
GGCTTTAGTAGCTTTGTGAAATTTAGTAAGAGTAAATGGAACGTCTACACCTTTGGAGAA
CGTATCATAAAGCCAAAGAATAAGCAAGGTTATCGGGAGGACAAAAGAATCAACTTGACC
TTCGAGATGAAGAAGTTACTTAACGAGTATAAGGTTTCTTTTGATCTTGAAAATAACTTG
ATTCCGAATCTCACGAGTGCCAACCTGAAGGATACTTTTTGGAAAGAGCTATTCTTTATC
TTCAAGACTACGCTGCAGCTCCGTAACAGCGTTACTAACGGTAAAGAAGATGTGCTCATC
TCTCCGGTCAAAAATGCGAAGGGTGAATTCTTCGTTTCGGGAACGCATAACAAGACTCTT
CCGCAAGATTGCGATGCGAACGGTGCATACCATATTGCGTTGAAAGGTCTGATGATACTC
GAACGTAACAACCTTGTACGTGAGGAGAAAGATACGAAAAAGATTATGGCGATTTCAAAC
GTGGATTGGTTCGAGTACGTGCAGAAACGTAGAGGCGTTCTGTAA
SEQ ATGAACAACTACGACGAATTCACCAAACTGTACCCGATCCAGAAAACCATCCGTTTCGAA
ID CTGAAACCGCAGGGTCGTACCATGGAACACCTGGAAACCTTCAACTTCTTCGAAGAAGAC
NO: CGTGACCGTGCGGAAAAATACAAAATCCTGAAAGAAGCGATCGACGAATACCACAAAAAA
43 TTCATCGACGAACACCTGACCAACATGTCTCTGGACTGGAACTCTCTGAAACAGATCTCT
GAAAAATACTACAAATCTCGTGAAGAAAAAGACAAAAAAGTTTTCCTGTCTGAACAGAAA
CGTATGCGTCAGGAAATCGTTTCTGAATTCAAAAAAGACGACCGTTTCAAAGACCTGTTC
TCTAAAAAACTGTTCTCTGAACTGCTGAAAGAAGAAATCTACAAAAAAGGTAACCACCAG
GAAATCGACGCGCTGAAATCTTTCGACAAATTCTCTGGTTACTTCATCGGTCTGCACGAA
AACCGTAAAAACATGTACTCTGACGGTGACGAAATCACCGCGATCTCTAACCGTATCGTT
AACGAAAACTTCCCGAAATTCCTGGACAACCTGCAGAAATACCAGGAAGCGCGTAAAAAA
TACCCGGAATGGATCATCAAAGCGGAATCTGCGCTGGTTGCGCACAACATCAAAATGGAC
GAAGTTTTCTCTCTGGAATACTTCAACAAAGTTCTGAACCAGGAAGGTATCCAGCGTTAC
AACCTGGCGCTGGGTGGTTACGTTACCAAATCTGGTGAAAAAATGATGGGTCTGAACGAC
GCGCTGAACCTGGCGCACCAGTCTGAAAAATCTTCTAAAGGTCGTATCCACATGACCCCG
CTGTTCAAACAGATCCTGTCTGAAAAAGAATCTTTCTCTTACATCCCGGACGTTTTCACC
GAAGACTCTCAGCTGCTGCCGTCTATCGGTGGTTTCTTCGCGCAGATCGAAAACGACAAA
GACGGTAACATCTTCGACCGTGCGCTGGAACTGATCTCTTCTTACGCGGAATACGACACC
GAACGTATCTACATCCGTCAGGCGGACATCAACCGTGTTTCTAACGTTATCTTCGGTGAA
TGGGGTACCCTGGGTGGTCTGATGCGTGAATACAAAGCGGACTCTATCAACGACATCAAC
CTGGAACGTACCTGCAAAAAAGTTGACAAATGGCTGGACTCTAAAGAATTCGCGCTGTCT
GACGTTCTGGAAGCGATCAAACGTACCGGTAACAACGACGCGTTCAACGAATACATCTCT
AAAATGCGTACCGCGCGTGAAAAAATCGACGCGGCGCGTAAAGAAATGAAATTCATCTCT
GAAAAAATCTCTGGTGACGAAGAATCTATCCACATCATCAAAACCCTGCTGGACTCTGTT
CAGCAGTTCCTGCACTTCTTCAACCTGTTCAAAGCGCGTCAGGACATCCCGCTGGACGGT
GCGTTCTACGCGGAATTCGACGAAGTTCACTCTAAACTGTTCGCGATCGTTCCGCTGTAC
AACAAAGTTCGTAACTACCTGACCAAAAACAACCTGAACACCAAAAAAATCAAACTGAAC
TTCAAAAACCCGACCCTGGCGAACGGTTGGGACCAGAACAAAGTTTACGACTACGCGTCT
CTGATCTTCCTGCGTGACGGTAACTACTACCTGGGTATCATCAACCCGAAACGTAAAAAA
AACATCAAATTCGAACAGGGTTCTGGTAACGGTCCGTTCTACCGTAAAATGGTTTACAAA
CAGATCCCGGGTCCGAACAAAAACCTGCCGCGTGTTTTCCTGACCTCTACCAAAGGTAAA
AAAGAATACAAACCGTCTAAAGAAATCATCGAAGGTTACGAAGCGGACAAACACATCCGT
GGTGACAAATTCGACCTGGACTTCTGCCACAAACTGATCGACTTCTTCAAAGAATCTATC
GAAAAACACAAAGACTGGTCTAAATTCAACTTCTACTTCTCTCCGACCGAATCTTACGGT
GACATCTCTGAATTCTACCTGGACGTTGAAAAACAGGGTTACCGTATGCACTTCGAAAAC
ATCTCTGCGGAAACCATCGACGAATACGTTGAAAAAGGTGACCTGTTCCTGTTCCAGATC
TACAACAAAGACTTCGTTAAAGCGGCGACCGGTAAAAAAGACATGCACACCATCTACTGG
AACGCGGCGTTCTCTCCGGAAAACCTGCAGGACGTTGTTGTTAAACTGAACGGTGAAGCG
GAACTGTTCTACCGTGACAAATCTGACATCAAAGAAATCGTTCACCGTGAAGGTGAAATC
CTGGTTAACCGTACCTACAACGGTCGTACCCCGGTTCCGGACAAAATCCACAAAAAACTG
ACCGACTACCACAACGGTCGTACCAAAGACCTGGGTGAAGCGAAAGAATACCTGGACAAA
GTTCGTTACTTCAAAGCGCACTACGACATCACCAAAGACCGTCGTTACCTGAACGACAAA
ATCTACTTCCACGTTCCGCTGACCCTGAACTTCAAAGCGAACGGTAAAAAAAACCTGAAC
AAAATGGTTATCGAAAAATTCCTGTCTGACGAAAAAGCGCACATCATCGGTATCGACCGT
GGTGAACGTAACCTGCTGTACTACTCTATCATCGACCGTTCTGGTAAAATCATCGACCAG
CAGTCTCTGAACGTTATCGACGGTTTCGACTACCGTGAAAAACTGAACCAGCGTGAAATC
GAAATGAAAGACGCGCGTCAGTCTTGGAACGCGATCGGTAAAATCAAAGACCTGAAAGAA
GGTTACCTGTCTAAAGCGGTTCACGAAATCACCAAAATGGCGATCCAGTACAACGCGATC
GTTGTTATGGAAGAACTGAACTACGGTTTCAAACGTGGTCGTTTCAAAGTTGAAAAACAG
ATCTACCAGAAATTCGAAAACATGCTGATCGACAAAATGAACTACCTGGTTTTCAAAGAC
GCGCCGGACGAATCTCCGGGTGGTGTTCTGAACGCGTACCAGCTGACCAACCCGCTGGAA
TCTTTCGCGAAACTGGGTAAACAGACCGGTATCCTGTTCTACGTTCCGGCGGCGTACACC
TCTAAAATCGACCCGACCACCGGTTTCGTTAACCTGTTCAACACCTCTTCTAAAACCAAC
GCGCAGGAACGTAAAGAATTCCTGCAGAAATTCGAATCTATCTCTTACTCTGCGAAAGAC
GGTGGTATCTTCGCGTTCGCGTTCGACTACCGTAAATTCGGTACCTCTAAAACCGACCAC
AAAAACGTTTGGACCGCGTACACCAACGGTGAACGTATGCGTTACATCAAAGAAAAAAAA
CGTAACGAACTGTTCGACCCGTCTAAAGAAATCAAAGAAGCGCTGACCTCTTCTGGTATC
AAATACGACGGTGGTCAGAACATCCTGCCGGACATCCTGCGTTCTAACAACAACGGTCTG
ATCTACACCATGTACTCTTCTTTCATCGCGGCGATCCAGATGCGTGTTTACGACGGTAAA
GAAGACTACATCATCTCTCCGATCAAAAACTCTAAAGGTGAATTCTTCCGTACCGACCCG
AAACGTCGTGAACTGCCGATCGACGCGGACGCGAACGGTGCGTACAACATCGCGCTGCGT
GGTGAACTGACCATGCGTGCGATCGCGGAAAAATTCGACCCGGACTCTGAAAAAATGGCG
AAACTGGAACTGAAACACAAAGACTGGTTCGAATTCATGCAGACCCGTGGTGACTAA
SEQ ATGACTAAAACATTTGATTCAGAGTTTTTTAATTTGTACTCGCTGCAAAAAACGGTACGC
ID TTTGAGTTAAAACCCGTGGGAGAAACCGCGTCATTTGTGGAAGACTTTAAAAACGAGGGC
NO: TTGAAACGTGTTGTGAGCGAAGATGAAAGGCGAGCCGTCGATTACCAGAAAGTTAAGGAA
44 ATAATTGACGATTACCATCGGGATTTCATTGAAGAAAGTTTAAATTATTTTCCGGAACAG
GTGAGTAAAGATGCTCTTGAGCAGGCGTTTCATCTTTATCAGAAACTGAAGGCAGCAAAA
GTTGAGGAAAGGGAAAAAGCGCTGAAAGAATGGGAAGCGCTGCAGAAAAAGCTACGTGAA
AAAGTGGTGAAATGCTTCTCGGACTCGAATAAAGCCCGCTTCTCAAGGATTGATAAAAAG
GAACTGATTAAGGAAGACCTGATAAATTGGTTGGTCGCCCAGAATCGCGAGGATGATATC
CCTACGGTCGAAACGTTTAACAACTTCACCACATATTTTACCGGCTTCCATGAGAATCGT
AAAAATATTTACTCCAAAGATGATCACGCCACCGCTATTAGCTTTCGCCTTATTCATGAA
AATCTTCCAAAGTTTTTTGACAACGTGATTAGCTTCAATAAGTTGAAAGAGGGTTTCCCT
GAATTAAAATTTGATAAAGTGAAAGAGGATTTAGAAGTAGATTATGATCTGAAGCATGCG
TTTGAAATAGAATATTTCGTTAACTTCGTGACCCAAGCGGGCATAGATCAGTATAATTAT
CTGTTAGGAGGGAAAACCCTGGAGGACGGGACGAAAAAACAAGGGATGAATGAGCAAATT
AATCTGTTCAAACAACAGCAAACGCGAGATAAAGCGCGTCAGATTCCCAAACTGATCCCC
CTGTTCAAACAGATTCTTAGCGAAAGGACTGAAAGCCAGTCCTTTATTCCTAAACAATTT
GAAAGTGATCAGGAGTTGTTCGATTCACTGCAGAAGTTACATAATAACTGCCAGGATAAA
TTCACCGTGCTGCAACAAGCCATTCTCGGTCTGGCAGAGGCGGATCTTAAGAAGGTCTTC
ATCAAAACCTCTGATTTAAATGCCTTATCTAACACCATTTTCGGGAATTACAGCGTCTTT
TCCGATGCACTGAACCTGTATAAAGAAAGCCTGAAAACGAAAAAAGCGCAGGAGGCTTTT
GAGAAACTACCGGCCCATTCTATTCACGACCTCATTCAATACTTGGAACAGTTCAATTCC
AGCCTGGACGCGGAAAAACAACAGAGCACCGACACCGTCCTGAACTACTTCATCAAGACC
GATGAATTATATTCTCGCTTCATTAAATCCACTAGCGAGGCTTTCACTCAGGTGCAGCCT
TTGTTCGAACTGGAAGCCCTGTCATCTAAGCGCCGCCCACCGGAATCGGAAGATGAAGGG
GCAAAAGGGCAGGAAGGCTTCGAGCAGATCAAGCGTATTAAAGCTTACCTGGATACGCTT
ATGGAAGCGGTACACTTTGCAAAGCCGTTGTATCTTGTTAAGGGTCGTAAAATGATCGAA
GGGCTCGATAAAGACCAGTCCTTTTATGAAGCGTTTGAAATGGCGTACCAAGAACTTGAA
TCGTTAATCATTCCTATCTATAACAAAGCGCGGAGCTATCTGTCGCGGAAACCTTTCAAG
GCCGATAAATTCAAGATTAATTTTGACAACAACACGCTACTGAGCGGATGGGATGCGAAC
AAGGAAACTGCTAACGCGTCCATTCTGTTTAAGAAAGACGGGTTATATTACCTTGGAATT
ATGCCGAAAGGTAAGACCTTTCTCTTTGACTACTTTGTATCGAGCGAGGATTCAGAGAAA
CTGAAACAGCGTCGCCAGAAGACCGCCGAAGAAGCTCTGGCGCAGGATGGTGAAAGTTAC
TTCGAAAAAATTCGTTATAAACTGTTACCAGGGGCTTCAAAGATGTTACCGAAAGTCTTT
TTTAGCAACAAAAATATTGGCTTTTACAACCCGTCGGATGACATTTTACGCATTCGCAAC
ACAGCCTCTCACACCAAAAACGGGACCCCTCAGAAAGGCCACTCAAAAGTTGAGTTTAAC
CTGAATGATTGTCATAAGATGATTGATTTCTTCAAATCATCAATTCAGAAACACCCGGAA
TGGGGGTCTTTTGGCTTTACGTTTTCTGATACCAGTGATTTTGAAGACATGAGTGCCTTC
TACCGGGAAGTAGAAAACCAGGGTTACGTAATTAGCTTTGACAAAATCAAAGAGACCTAT
ATACAGAGCCAGGTGGAACAGGGTAATCTCTACTTATTCCAGATTTATAACAAGGATTTC
TCGCCCTACAGCAAAGGCAAACCAAACCTGCATACTCTGTACTGGAAAGCCCTGTTTGAA
GAAGCGAACCTGAATAACGTAGTGGCGAAGTTGAACGGTGAAGCGGAAATCTTCTTCCGT
CGTCACTCCATTAAGGCCTCTGATAAAGTTGTCCATCCGGCAAATCAGGCCATTGATAAT
AAGAATCCACACACGGAAAAAACGCAGTCAACCTTTGAATATGACCTCGTTAAAGACAAA
CGCTACACGCAAGATAAGTTCTTTTTCCACGTCCCAATCAGCCTCAACTTTAAAGCACAA
GGGGTTTCAAAGTTTAATGATAAAGTCAATGGGTTCCTCAAGGGCAACCCGGATGTCAAC
ATTATAGGTATAGACAGGGGCGAACGCCATCTGCTTTACTTTACCGTAGTGAATCAGAAA
GGTGAAATACTGGTTCAGGAATCATTAAATACCTTGATGTCGGACAAAGGGCACGTTAAT
GATTACCAGCAGAAACTGGATAAAAAAGAACAGGAACGTGATGCTGCGCGTAAATCGTGG
ACCACGGTTGAGAACATTAAAGAGCTGAAAGAGGGGTATCTAAGCCATGTGGTACACAAA
CTGGCGCACCTCATCATTAAATATAACGCAATAGTCTGCCTAGAAGACTTGAATTTTGGC
TTTAAACGCGGCCGCTTCAAAGTGGAAAAACAAGTTTATCAAAAATTTGAAAAGGCGCTT
ATAGATAAACTGAATTATCTGGTTTTTAAAGAAAAGGAACTTGGTGAGGTAGGGCACTAC
TTGACAGCTTATCAACTGACGGCCCCGTTCGAATCATTCAAAAAACTGGGCAAACAGTCT
GGCATTCTGTTTTACGTGCCGGCAGATTATACTTCAAAAATCGATCCAACAACTGGCTTT
GTGAACTTCCTGGACCTGAGATATCAGTCTGTAGAAAAAGCTAAACAACTTCTTAGCGAT
TTTAATGCCATTCGTTTTAACAGCGTTCAGAATTACTTTGAATTCGAAATTGACTATAAA
AAACTTACTCCGAAACGTAAAGTCGGAACCCAAAGTAAATGGGTAATTTGTACGTATGGC
GATGTCAGGTATCAGAACCGTCGGAATCAAAAAGGTCATTGGGAGACCGAAGAAGTGAAC
GTGACCGAAAAGCTGAAGGCTCTGTTCGCCAGCGATTCAAAAACTACAACTGTGATCGAT
TACGCAAATGATGATAACCTGATAGATGTGATTTTAGAGCAGGATAAAGCCAGCTTTTTT
AAAGAACTGTTGTGGCTCCTGAAACTTACGATGACCTTACGACATTCCAAGATCAAATCG
GAAGATGATTTTATTCTGTCACCGGTCAAGAATGAGCAGGGTGAATTCTATGATAGTAGG
AAAGCCGGCGAAGTGTGGCCGAAAGACGCCGACGCCAATGGCGCCTATCATATCGCGCTC
AAAGGGCTTTGGAATTTGCAGCAGATTAACCAGTGGGAAAAAGGTAAAACCCTGAATCTG
GCTATCAAAAACCAGGATTGGTTTAGCTTTATCCAAGAGAAACCGTATCAGGAATGA
SEQ ATGCATACAGGCGGTCTTCTTAGTATGGACGCGAAAGAGTTCACAGGTCAGTATCCGTTG
ID TCGAAAACATTACGATTCGAACTTCGGCCCATCGGCCGCACGTGGGATAACCTGGAGGCC
NO: TCAGGCTACTTAGCGGAAGACCGCCATCGTGCCGAATGTTATCCTCGTGCGAAAGAGTTA
45 TTGGATGACAACCATCGTGCCTTCCTGAATCGTGTGTTGCCACAAATCGATATGGATTGG
CACCCGATTGCGGAGGCCTTTTGTAAGGTACATAAAAACCCTGGTAATAAAGAACTTGCC
CAGGATTACAACCTTCAGTTGTCAAAGCGCCGTAAGGAGATCAGCGCATATCTTCAGGAT
GCAGATGGCTATAAAGGCCTGTTCGCGAAGCCCGCCTTAGACGAAGCTATGAAAATTGCG
AAAGAAAACGGGAACGAAAGTGATATTGAGGTTCTCGAAGCGTTTAACGGTTTTAGCGTA
TACTTCACCGGTTATCATGAGTCACGCGAGAACATTTATAGCGATGAGGATATGGTGAGC
GTAGCCTACCGAATTACTGAGGATAATTTCCCGCGCTTTGTCTCAAACGCTTTGATCTTT
GATAAATTAAACGAAAGCCATCCGGATATTATCTCTGAAGTATCGGGCAATCTTGGAGTT
GATGACATTGGTAAGTACTTTGACGTGTCGAACTATAACAATTTTCTTTCCCAGGCCGGT
ATAGATGACTACAATCACATTATTGGCGGCCATACAACCGAAGACGGACTGATACAAGCG
TTTAATGTCGTATTGAACTTACGTCACCAAAAAGACCCTGGCTTTGAAAAAATTCAGTTC
AAACAGCTCTACAAACAAATCCTGAGCGTGCGTACCAGCAAAAGCTACATCCCGAAACAG
TTTGACAACTCTAAGGAGATGGTTGACTGCATTTGCGATTATGTCAGCAAAATAGAGAAA
TCCGAAACAGTAGAACGGGCCCTGAAACTAGTCCGTAATATCAGTTCTTTCGACTTGCGC
GGGATCTTTGTCAATAAAAAGAACTTGCGCATACTGAGCAACAAACTGATAGGAGATTGG
GACGCGATCGAAACCGCATTGATGCATAGTTCTTCATCAGAAAACGATAAGAAAAGCGTA
TATGATAGCGCGGAGGCTTTTACGTTGGATGACATCTTTTCAAGCGTGAAAAAATTTTCT
GATGCCTCTGCCGAAGATATTGGCAACAGGGCGGAAGACATCTGTAGAGTGATAAGTGAG
ACGGCCCCTTTTATCAACGATCTGCGAGCGGTGGACCTGGATAGCCTGAACGACGATGGT
TATGAAGCGGCCGTCTCAAAAATTCGGGAGTCGCTGGAGCCTTATATGGATCTTTTCCAT
GAACTGGAAATTTTCTCGGTTGGCGATGAGTTCCCAAAATGCGCAGCATTTTACAGCGAA
CTGGAGGAAGTCAGCGAACAGCTGATCGAAATTATTCCGTTATTCAACAAGGCGCGTTCG
TTCTGCACCCGGAAACGCTATAGCACCGATAAGATTAAAGTGAACTTAAAATTCCCGACC
TTGGCGGACGGGTGGGACCTGAACAAAGAGAGAGACAACAAAGCCGCGATTCTGCGGAAA
GACGGTAAGTATTATCTGGCAATTCTGGATATGAAGAAAGATCTGTCAAGCATTAGGACC
AGCGACGAAGATGAATCCAGCTTCGAAAAGATGGAGTATAAACTGTTACCGAGTCCAGTA
AAAATGCTGCCAAAGATATTCGTAAAATCGAAAGCCGCTAAGGAAAAATATGGCCTGACA
GATCGTATGCTTGAATGCTACGATAAAGGTATGCATAAGTCGGGTAGTGCGTTTGATCTT
GGCTTTTGCCATGAACTCATTGATTATTACAAGCGTTGTATCGCGGAGTACCCAGGCTGG
GATGTGTTCGATTTCAAGTTTCGCGAAACTTCCGATTATGGGTCCATGAAAGAGTTCAAT
GAAGATGTGGCCGGAGCCGGTTACTATATGAGTCTGAGAAAAATTCCGTGCAGCGAAGTG
TACCGTCTGTTAGACGAGAAATCGATTTATCTATTTCAAATTTATAACAAAGATTACTCT
GAAAATGCACATGGTAATAAGAACATGCATACCATGTACTGGGAGGGTCTCTTTTCCCCG
CAAAACCTGGAGTCGCCCGTTTTCAAGTTGTCGGGTGGGGCAGAACTTTTCTTTCGAAAA
TCCTCAATCCCTAACGATGCCAAAACAGTACACCCGAAAGGCTCAGTGCTGGTTCCACGT
AATGATGTTAACGGTCGGCGTATTCCAGATTCAATCTACCGCGAACTGACACGCTATTTT
AACCGTGGCGATTGCCGAATCAGTGACGAAGCCAAAAGTTATCTTGACAAGGTTAAGACT
AAAAAAGCGGACCATGACATTGTGAAAGATCGCCGCTTTACCGTGGATAAAATGATGTTC
CACGTCCCGATTGCGATGAACTTTAAGGCGATCAGTAAACCGAACTTAAACAAAAAAGTC
ATTGATGGCATCATTGATGATCAGGATCTGAAAATCATTGGTATTGATCGTGGCGAGCGG
AACTTAATTTACGTCACGATGGTTGACAGAAAAGGGAATATCTTATATCAGGATTCTCTT
AACATCCTCAATGGCTACGACTATCGTAAAGCTCTGGATGTGCGCGAATATGACAACAAG
GAAGCGCGTCGTAACTGGACTAAAGTGGAGGGCATTCGCAAAATGAAGGAAGGCTATCTG
TCATTAGCGGTCTCGAAATTAGCGGATATGATTATCGAAAATAACGCCATCATCGTTATG
GAGGACCTGAACCACGGATTCAAAGCGGGCCGCTCAAAGATTGAAAAACAAGTTTATCAG
AAATTTGAGAGTATGCTGATTAACAAACTGGGCTATATGGTGTTAAAAGACAAGTCAATT
GACCAATCAGGTGGCGCGCTGCATGGATACCAGCTGGCGAACCATGTTACCACCTTAGCA
TCAGTTGGAAAGCAGTGTGGGGTTATCTTTTATATACCGGCAGCGTTCACTAGTAAAATA
GATCCGACCACTGGTTTCGCCGATCTCTTTGCCCTGAGTAACGTTAAAAACGTAGCGAGC
ATGCGTGAATTCTTTTCCAAAATGAAATCTGTCATTTATGATAAAGCTGAAGGCAAATTC
GCATTCACCTTTGATTACTTGGATTACAACGTGAAGAGCGAATGTGGTCGTACGCTGTGG
ACCGTTTACACCGTTGGTGAGCGCTTCACCTATTCCCGTGTGAACCGCGAATATGTACGT
AAAGTCCCCACCGATATTATCTATGATGCCCTCCAGAAAGCAGGCATTAGCGTCGAAGGA
GACTTAAGGGACAGAATTGCCGAAAGCGATGGCGATACGCTGAAGTCTATTTTTTACGCA
TTCAAATACGCGCTAGATATGCGCGTTGAGAATCGCGAGGAAGACTACATTCAATCACCT
GTGAAAAATGCCTCTGGGGAATTTTTTTGTTCAAAAAATGCTGGTAAAAGCCTCCCACAA
GATAGCGATGCAAACGGTGCATATAACATTGCCCTGAAAGGTATTCTTCAATTACGCATG
CTGTCTGAGCAGTACGACCCCAACGCGGAATCTATTAGACTTCCGCTGATAACCAATAAA
GCCTGGCTGACATTCATGCAGTCTGGCATGAAGACCTGGAAAAATTAG
SEQ ATGGATAGTTTAAAAGATTTTACGAATCTATATCCCGTAAGCAAAACTCTTCGTTTTGAA
ID CTGAAACCTGTTGGAAAAACGTTGGAGAATATCGAGAAAGCGGGCATCCTGAAAGAAGAC
NO: GAGCACCGTGCCGAAAGCTACAGGCGTGTCAAAAAGATTATCGATACTTATCACAAAGTG
46 TTCATTGATAGCAGTCTGGAGAACATGGCAAAAATGGGCATAGAAAATGAAATCAAAGCA
ATGCTGCAGAGCTTTTGCGAGCTCTACAAGAAAGATCACCGAACGGAAGGTGAAGATAAA
GCACTGGACAAAATTCGCGCCGTTCTTCGCGGTCTGATTGTTGGCGCGTTCACCGGCGTG
TGCGGCCGCCGTGAAAACACCGTGCAGAACGAAAAGTACGAGTCGCTGTTCAAAGAAAAA
CTGATAAAAGAAATTTTGCCTGACTTTGTGCTTTCGACCGAAGCGGAATCCCTGCCATTT
TCTGTCGAAGAAGCGACCCGCAGCCTGAAAGAATTTGACTCATTCACAAGTTACTTTGCA
GGCTTCTACGAAAACCGTAAAAACATCTACAGCACGAAGCCACAGAGCACGGCTATTGCT
TATCGCCTGATTCATGAGAACCTGCCGAAGTTCATCGATAACATCCTTGTTTTTCAAAAA
ATTAAAGAGCCGATTGCGAAAGAGTTAGAACATATTCGAGCTGACTTTTCTGCGGGTGGG
TACATTAAAAAAGATGAGCGGCTGGAAGACATCTTCAGTCTAAACTATTATATCCACGTT
CTGTCGCAGGCAGGCATTGAGAAATATAATGCGCTGATTGGTAAGATTGTCACAGAAGGC
GATGGTGAGATGAAAGGTCTTAATGAACATATCAATCTGTATAACCAGCAGCGTGGTCGC
GAAGACCGTCTTCCACTGTTCCGCCCACTGTATAAACAGATCCTGTCTGACCGGGAACAG
CTGTCCTACCTGCCGGAAAGCTTTGAAAAGGATGAAGAGCTACTTCGCGCATTAAAGGAG
TTTTACGACCATATTGCGGAAGACATTTTGGGTAGAACGCAGCAACTGATGACGTCAATT
TCTGAATACGATCTGAGTAGAATCTACGTTAGGAATGATAGCCAGCTGACCGATATTAGC
AAAAAAATGCTGGGCGACTGGAACGCTATCTATATGGCACGTGAACGTGCATATGATCAT
GAACAAGCACCGAAACGTATAACCGCGAAATATGAGCGTGATCGCATTAAGGCGCTAAAG
GGAGAAGAAAGCATCTCACTCGCAAACCTGAACTCCTGTATCGCTTTCTTAGATAACGTG
CGCGATTGTCGCGTCGACACGTATCTGTCAACCCTTGGGCAGAAAGAGGGTCCACATGGT
CTGTCTAACCTGGTGGAAAATGTCTTTGCGAGTTACCATGAAGCGGAACAACTGCTGTCT
TTTCCATACCCCGAAGAAAACAATCTAATACAGGATAAAGATAACGTGGTGTTAATCAAA
AACCTGCTGGACAACATCAGCGATCTGCAACGTTTCCTGAAACCTTTGTGGGGTATGGGT
GACGAGCCAGACAAAGACGAACGTTTTTATGGTGAGTATAATTATATACGTGGCGCCCTT
GACCAAGTTATTCCGCTGTATAACAAAGTACGGAACTATCTGACCCGTAAGCCATATTCT
ACCCGTAAAGTGAAACTGAACTTTGGCAACTCGCAACTGCTGTCGGGTTGGGATCGTAAC
AAAGAAAAAGATAATAGTTGTGTTATCCTGCGTAAGGGACAAAATTTTTACCTCGCGATT
ATGAACAACAGACACAAGCGTTCATTTGAAAATAAGGTTCTGCCGGAGTATAAAGAGGGC
GAACCGTACTTCGAGAAAATGGATTATAAGTTCTTACCAGACCCTAATAAGATGTTACCG
AAAGTCTTTCTTTCGAAAAAAGGCATAGAAATCTATAAGCCGTCCCCGAAATTACTCGAA
CAGTATGGGCACGGGACCCACAAGAAAGGGGATACTTTTAGCATGGACGATCTGCACGAA
CTGATCGATTTTTTTAAACACTCCATCGAAGCCCATGAAGACTGGAAACAGTTTGGGTTC
AAGTTCTCTGATACAGCCACATACGAGAATGTGTCTAGTTTTTATCGGGAAGTGGAGGAT
CAGGGCTACAAACTTAGTTTTCGTAAAGTTTCAGAGAGTTATGTTTATAGTTTAATTGAT
CAGGGAAAACTTTACCTGTTCCAGATCTACAACAAAGATTTCTCGCCATGTAGTAAGGGT
ACCCCGAATCTGCATACACTCTATTGGAGAATGTTATTCGATGAGCGTAACTTAGCGGAT
GTCATTTATAAATTGGACGGGAAAGCAGAGATCTTTTTTCGTGAAAAATCACTGAAGAAT
GACCACCCGACTCATCCGGCCGGGAAACCGATCAAAAAAAAATCCCGCCAGAAAAAAGGA
GAAGAGTCTCTGTTTGAATATGATCTGGTGAAAGACCGTCATTACACTATGGATAAATTT
CAATTTCATGTTCCAATTACAATGAACTTCAAATGTTCGGCGGGTTCCAAAGTAAATGAT
ATGGTAAACGCCCATATTCGCGAAGCGAAAGATATGCATGTTATTGGCATCGATAGAGGC
GAAAGAAACCTGCTTTATATTTGCGTAATTGACAGCCGTGGTACCATTCTGGACCAGATC
TCTTTAAACACCATCAATGACATCGATTATCACGACCTGTTGGAGTCTCGGGACAAGGAC
CGCCAGCAGGAGCGCCGTAATTGGCAGACAATTGAAGGCATAAAAGAATTAAAACAGGGT
TACCTTTCCCAGGCCGTACACCGCATAGCGGAACTGATGGTGGCCTACAAAGCCGTAGTT
GCCCTGGAAGACTTGAATATGGGGTTTAAACGTGGCCGTCAAAAAGTCGAGAGCAGCGTG
TATCAGCAATTTGAAAAACAGTTGATTGACAAGTTGAATTATTTGGTTGATAAAAAGAAA
CGTCCAGAAGATATTGGTGGCTTACTGCGTGCATACCAGTTTACGGCACCTTTTAAGTCC
TTCAAAGAAATGGGTAAACAGAACGGGTTTCTGTTTTACATCCCGGCCTGGAATACATCC
AACATCGATCCTACCACCGGGTTTGTCAACCTGTTTCATGCACAATATGAAAACGTGGAT
AAAGCGAAGAGTTTTTTCCAAAAATTCGATAGTATTTCGTATAACCCAAAAAAAGATTGG
TTTGAGTTTGCGTTCGATTATAAAAATTTTACTAAAAAGGCTGAGGGATCCCGCAGTATG
TGGATCCTCTGCACCCATGGCAGTCGTATTAAAAATTTTCGTAATTCGCAAAAGAATGGC
CAGTGGGACTCGGAAGAGTTTGCCCTGACCGAAGCGTTCAAATCGCTGTTTGTACGCTAC
GAAATTGACTACACAGCAGATCTGAAAACAGCCATCGTCGATGAAAAACAGAAAGATTTT
TTTGTAGATCTCCTAAAACTGTTCAAACTGACTGTTCAGATGCGCAATTCCTGGAAAGAG
AAAGACCTGGATTATCTGATTAGCCCGGTAGCCGGTGCTGATGGACGATTTTTCGATACT
CGTGAAGGTAACAAAAGTCTCCCGAAAGATGCTGATGCCAATGGTGCATACAATATTGCA
TTAAAGGGGCTATGGGCCTTGCGACAGATCCGCCAGACCAGCGAAGGCGGCAAGCTGAAA
TTGGCCATATCGAATAAGGAATGGTTACAATTTGTTCAGGAACGTAGCTATGAAAAAGAT
TGA
SEQ ATGAACAACGGCACAAATAATTTTCAGAACTTCATCGGGATCTCAAGTTTGCAGAAAACG
ID CTGCGCAATGCTCTGATCCCCACGGAAACCACGCAACAGTTCATCGTCAAGAACGGAATA
NO: ATTAAAGAAGATGAGTTACGTGGCGAGAACCGCCAGATTCTGAAAGATATCATGGATGAC
47 TACTACCGCGGATTCATCTCTGAGACTCTGAGTTCTATTGATGACATAGATTGGACTAGC
CTGTTCGAAAAAATGGAAATTCAGCTGAAAAATGGTGATAATAAAGATACCTTAATTAAG
GAACAGACAGAGTATCGGAAAGCAATCCATAAAAAATTTGCGAACGACGATCGGTTTAAG
AACATGTTTAGCGCCAAACTGATTAGTGACATATTACCTGAATTTGTCATCCACAACAAT
AATTATTCGGCATCAGAGAAAGAGGAAAAAACCCAGGTGATAAAATTGTTTTCGCGCTTT
GCGACTAGCTTTAAAGATTACTTCAAGAACCGTGCAAATTGCTTTTCAGCGGACGATATT
TCATCAAGCAGCTGCCATCGCATCGTCAACGACAATGCAGAGATATTCTTTTCAAATGCG
CTGGTCTACCGCCGGATCGTAAAATCGCTGAGCAATGACGATATCAACAAAATTTCGGGC
GATATGAAAGATTCATTAAAAGAAATGAGTCTGGAAGAAATATATTCTTACGAGAAGTAT
GGGGAATTTATTACCCAGGAAGGCATTAGCTTCTATAATGATATCTGTGGGAAAGTGAAT
TCTTTTATGAACCTGTATTGTCAGAAAAATAAAGAAAACAAAAATTTATACAAACTTCAG
AAACTTCACAAACAGATTCTATGCATTGCGGACACTAGCTATGAGGTCCCGTATAAATTT
GAAAGTGACGAGGAAGTGTACCAATCAGTTAACGGCTTCCTTGATAACATTAGCAGCAAA
CATATAGTCGAAAGATTACGCAAAATCGGCGATAACTATAACGGCTACAACCTGGATAAA
ATTTATATCGTGTCCAAATTTTACGAGAGCGTTAGCCAAAAAACCTACCGCGACTGGGAA
ACAATTAATACCGCCCTCGAAATTCATTACAATAATATCTTGCCGGGTAACGGTAAAAGT
AAAGCCGACAAAGTAAAAAAAGCGGTTAAGAATGATTTACAGAAATCCATCACCGAAATA
AATGAACTAGTGTCAAACTATAAGCTGTGCAGTGACGACAACATCAAAGCGGAGACTTAT
ATACATGAGATTAGCCATATCTTGAATAACTTTGAAGCACAGGAATTGAAATACAATCCG
GAAATTCACCTAGTTGAATCCGAGCTCAAAGCGAGTGAGCTTAAAAACGTGCTGGACGTG
ATCATGAATGCGTTTCATTGGTGTTCGGTTTTTATGACTGAGGAACTTGTTGATAAAGAC
AACAATTTTTATGCGGAACTGGAGGAGATTTACGATGAAATTTATCCAGTAATTAGTCTG
TACAACCTGGTTCGTAACTACGTTACCCAGAAACCGTACAGCACGAAAAAGATTAAATTG
AACTTTGGAATACCGACGTTAGCAGACGGTTGGTCAAAGTCCAAAGAGTATTCTAATAAC
GCTATCATACTGATGCGCGACAATCTGTATTATCTGGGCATCTTTAATGCGAAGAATAAA
CCGGACAAGAAGATTATCGAGGGTAATACGTCAGAAAATAAGGGTGACTACAAAAAGATG
ATTTATAATTTGCTCCCGGGTCCCAACAAAATGATCCCGAAAGTTTTCTTGAGCAGCAAG
ACGGGGGTGGAAACGTATAAACCGAGCGCCTATATCCTAGAGGGGTATAAACAGAATAAA
CATATCAAGTCTTCAAAAGACTTTGATATCACTTTCTGTCATGATCTGATCGACTACTTC
AAAAACTGTATTGCAATTCATCCCGAGTGGAAAAACTTCGGTTTTGATTTTAGCGACACC
AGTACTTATGAAGACATTTCCGGGTTTTATCGTGAGGTAGAGTTACAAGGTTACAAGATT
GATTGGACATACATTAGCGAAAAAGACATTGATCTGCTGCAGGAAAAAGGTCAACTGTAT
CTGTTCCAGATATATAACAAAGATTTTTCGAAAAAATCAACCGGGAATGACAACCTTCAC
ACCATGTACCTGAAAAATCTTTTCTCAGAAGAAAATCTTAAGGATATCGTCCTGAAACTT
AACGGCGAAGCGGAAATCTTCTTCAGGAAGAGCAGCATAAAGAACCCAATCATTCATAAA
AAAGGCTCGATTTTAGTCAACCGTACCTACGAAGCAGAAGAAAAAGACCAGTTTGGCAAC
ATTCAAATTGTGCGTAAAAATATTCCGGAAAACATTTATCAGGAGCTGTACAAATACTTC
AACGATAAAAGCGACAAAGAGCTGTCTGATGAAGCAGCCAAACTGAAGAATGTAGTGGGA
CACCACGAGGCAGCGACGAATATAGTCAAGGACTATCGCTACACGTATGATAAATACTTC
CTTCATATGCCTATTACGATCAATTTCAAAGCCAATAAAACGGGTTTTATTAATGATAGG
ATCTTACAGTATATCGCTAAAGAAAAAGACTTACATGTGATCGGCATTGATCGGGGCGAG
CGTAACCTGATCTACGTGTCCGTGATTGATACTTGTGGTAATATAGTTGAACAGAAAAGC
TTTAACATTGTAAACGGCTACGACTATCAGATAAAACTGAAACAACAGGAGGGCGCTAGA
CAGATTGCGCGGAAAGAATGGAAAGAAATTGGTAAAATTAAAGAGATCAAAGAGGGCTAC
CTGAGCTTAGTAATCCACGAGATCTCTAAAATGGTAATCAAATACAATGCAATTATAGCG
ATGGAGGATTTGTCTTATGGTTTTAAAAAAGGGCGCTTTAAGGTCGAACGGCAAGTTTAC
CAGAAATTTGAAACCATGCTCATCAATAAACTCAACTATCTGGTATTTAAAGATATTTCG
ATTACCGAGAATGGCGGTCTCCTGAAAGGTTATCAGCTGACATACATTCCTGATAAACTT
AAAAACGTGGGTCATCAGTGCGGCTGCATTTTTTATGTGCCTGCTGCATACACGAGCAAA
ATTGATCCGACCACCGGCTTTGTGAATATCTTTAAATTTAAAGACCTGACAGTGGACGCA
AAACGTGAATTCATTAAAAAATTTGACTCAATTCGTTATGACAGTGAAAAAAATCTGTTC
TGCTTTACATTTGACTACAATAACTTTATTACGCAAAACACGGTCATGAGCAAATCATCG
TGGAGTGTGTATACATACGGCGTGCGCATCAAACGTCGCTTTGTGAACGGCCGCTTCTCA
AACGAAAGTGATACCATTGACATAACCAAAGATATGGAGAAAACGTTGGAAATGACGGAC
ATTAACTGGCGCGATGGCCACGATCTTCGTCAAGACATTATAGATTATGAAATTGTTCAG
CACATATTCGAAATTTTCCGTTTAACAGTGCAAATGCGTAACTCCTTGTCTGAACTGGAG
GACCGTGATTACGATCGTCTCATTTCACCTGTACTGAACGAAAATAACATTTTTTATGAC
AGCGCGAAAGCGGGGGATGCACTTCCTAAGGATGCCGATGCAAATGGTGCGTATTGTATT
GCATTAAAAGGGTTATATGAAATTAAACAAATTACCGAAAATTGGAAAGAAGATGGTAAA
TTTTCGCGCGATAAACTCAAAATCAGCAATAAAGATTGGTTCGACTTTATCCAGAATAAG
CGCTATCTCTAA
SEQ ATGACCAATAAATTCACTAACCAGTATTCTCTCTCTAAGACCCTGCGCTTTGAACTGATT
ID CCGCAGGGGAAAACCTTGGAGTTCATTCAAGAAAAAGGCCTCTTGTCTCAGGATAAACAG
NO: AGGGCTGAATCTTACCAAGAAATGAAGAAAACTATTGATAAGTTTCATAAATATTTCATT
48 GATTTAGCCTTGTCTAACGCCAAATTAACTCACTTGGAAACGTATCTGGAGTTATACAAC
AAATCTGCCGAAACTAAGAAAGAACAGAAATTTAAAGACGATTTGAAAAAAGTACAGGAC
AATCTGCGTAAAGAAATTGTCAAATCCTTCAGTGACGGCGATGCTAAAAGCATTTTTGCC
ATTCTGGACAAAAAAGAGTTGATTACTGTGGAATTAGAAAAGTGGTTTGAAAACAATGAG
CAGAAAGACATCTACTTCGATGAGAAATTCAAAACTTTCACCACCTATTTTACAGGATTT
CATCAAAACCGGAAGAACATGTACTCAGTAGAACCGAACTCCACGGCCATTGCGTATCGT
TTGATCCATGAGAATCTGCCTAAATTTCTGGAGAATGCGAAAGCCTTTGAAAAGATTAAG
CAGGTCGAATCGCTGCAAGTGAATTTTCGTGAACTCATGGGCGAATTTGGTGACGAAGGT
CTAATCTTCGTTAACGAACTGGAAGAAATGTTTCAGATTAATTACTACAATGACGTGCTA
TCGCAGAACGGTATCACAATCTACAATAGTATTATCTCAGGGTTCACAAAAAACGATATA
AAATACAAAGGCCTGAACGAGTATATCAATAACTACAACCAAACAAAGGACAAAAAGGAT
AGGCTTCCGAAACTGAAGCAGTTATACAAACAGATTTTATCTGACAGAATCTCCCTGAGC
TTTCTGCCGGATGCTTTCACTGATGGGAAGCAGGTTCTGAAAGCGATTTTCGATTTTTAT
AAGATTAACTTACTGAGCTACACGATTGAAGGTCAAGAAGAATCTCAAAACTTACTGCTC
TTGATCCGTCAAACCATTGAAAATCTATCATCGTTCGATACGCAGAAAATCTACCTCAAA
AACGATACTCACCTGACTACGATCTCTCAGCAGGTTTTCGGGGATTTTAGTGTATTTTCA
ACAGCTCTGAACTACTGGTATGAAACCAAAGTCAATCCGAAATTCGAGACGGAATATTCT
AAGGCCAACGAAAAAAAACGTGAGATTCTTGATAAAGCTAAAGCCGTATTTACTAAACAG
GATTACTTTTCTATTGCTTTCCTGCAGGAAGTTTTATCGGAGTATATCCTGACCCTGGAT
CATACATCTGATATCGTTAAAAAACACAGCAGCAATTGCATCGCTGACTATTTCAAAAAC
CACTTTGTCGCCAAAAAAGAAAACGAAACAGACAAGACTTTCGATTTCATTGCTAACATC
ACCGCAAAATACCAGTGTATTCAGGGTATCTTGGAAAACGCCGACCAATACGAAGACGAA
CTGAAACAAGATCAGAAGCTGATCGATAATTTAAAATTCTTCTTAGATGCAATCCTGGAG
CTGCTGCACTTCATCAAACCGCTTCATTTAAAGAGCGAGTCCATTACCGAAAAGGACACC
GCCTTCTATGACGTTTTTGAAAATTATTATGAAGCCCTCTCCTTGCTGACTCCGCTGTAT
AATATGGTACGCAATTACGTAACCCAGAAACCATATTCTACCGAAAAAATTAAACTGAAC
TTTGAAAACGCACAGCTGCTCAACGGTTGGGACGCGAATAAAGAAGGTGACTACCTCACC
ACCATCCTGAAAAAAGATGGTAACTATTTTCTGGCAATTATGGATAAGAAACATAATAAA
GCATTCCAGAAATTTCCTGAAGGGAAAGAAAATTACGAAAAGATGGTGTACAAACTCTTA
CCTGGAGTTAACAAAATGTTGCCGAAAGTATTTTTTAGTAATAAGAACATCGCGTACTTT
AACCCGTCCAAAGAACTGCTGGAAAATTATAAAAAGGAGACGCATAAGAAAGGGGATACC
TTTAACCTGGAACATTGCCATACCTTAATAGACTTCTTCAAGGATTCCCTGAATAAACAC
GAGGATTGGAAATATTTCGATTTTCAGTTTAGTGAGACCAAGTCATACCAGGATCTTAGC
GGCTTTTATCGCGAAGTAGAACACCAAGGCTATAAAATTAACTTCAAAAACATCGACAGC
GAATACATCGACGGTTTAGTTAACGAGGGCAAACTGTTTCTGTTCCAGATCTATTCAAAG
GATTTTAGCCCGTTCTCTAAAGGCAAACCAAATATGCATACGTTGTACTGGAAAGCACTG
TTTGAAGAGCAAAACCTGCAGAATGTGATTTATAAACTGAACGGCCAAGCTGAGATTTTT
TTCCGTAAAGCCTCGATTAAACCGAAAAATATCATCCTTCATAAGAAGAAAATAAAGATC
GCTAAAAAACACTTCATAGATAAAAAAACCAAAACCTCCGAAATAGTGCCTGTTCAAACA
ATTAAGAACTTGAATATGTACTACCAGGGCAAGATATCGGAAAAGGAGTTGACTCAAGAC
GATCTTCGCTATATCGATAACTTTTCGATTTTTAACGAAAAAAACAAGACGATCGACATC
ATCAAAGATAAACGCTTCACTGTAGATAAGTTCCAGTTTCATGTGCCGATTACTATGAAC
TTCAAAGCTACCGGGGGTAGCTATATCAACCAAACGGTGTTGGAATACCTGCAGAATAAC
CCGGAAGTCAAAATCATTGGGCTGGACCGCGGAGAACGTCACCTTGTGTACTTGACCTTA
ATCGATCAGCAAGGCAACATCTTAAAACAAGAATCGCTGAATACCATTACGGATTCAAAG
ATTAGCACCCCGTATCATAAGCTGCTCGATAACAAGGAGAATGAGCGCGACCTGGCCCGT
AAAAACTGGGGCACGGTGGAAAACATTAAGGAGTTAAAGGAGGGTTATATTTCCCAGGTA
GTGCATAAGATCGCCACTCTCATGCTCGAGGAAAATGCGATCGTTGTCATGGAAGACTTA
AACTTCGGATTTAAACGTGGGCGATTTAAAGTAGAGAAACAAATCTACCAGAAGTTAGAA
AAAATGCTGATTGACAAATTAAATTACTTGGTCCTAAAAGACAAACAGCCGCAAGAATTG
GGTGGATTATACAACGCCCTCCAACTTACCAATAAATTCGAAAGTTTTCAGAAAATGGGT
AAACAGTCAGGCTTTCTTTTTTATGTTCCTGCGTGGAACACATCCAAAATCGACCCTACA
ACCGGCTTCGTCAATTACTTCTATACTAAATATGAAAACGTCGACAAAGCAAAAGCATTC
TTTGAAAAGTTCGAAGCAATACGTTTTAACGCTGAGAAAAAATATTTCGAGTTCGAAGTC
AAGAAATACTCAGACTTTAACCCCAAAGCTGAGGGCACACAGCAAGCGTGGACAATCTGC
ACCTACGGCGAGCGCATCGAAACGAAGCGTCAAAAAGATCAGAATAACAAATTTGTTTCA
ACACCTATCAACCTGACCGAGAAGATTGAAGACTTCTTAGGTAAAAATCAGATTGTTTAT
GGCGACGGTAACTGTATAAAATCTCAAATAGCCTCAAAGGATGATAAAGCATTTTTCGAA
ACATTATTATATTGGTTCAAAATGACACTGCAGATGCGCAATAGTGAGACGCGTACAGAT
ATTGATTATCTTATCAGCCCGGTCATGAACGACAACGGTACTTTTTACAACTCCAGAGAC
TATGAAAAACTTGAGAATCCAACTCTCCCCAAAGATGCTGATGCGAACGGTGCTTATCAC
ATCGCGAAAAAAGGTCTGATGCTGCTGAACAAAATCGACCAAGCCGATCTGACTAAGAAA
GTTGACCTAAGCATTTCAAATCGGGACTGGTTACAGTTTGTTCAAAAGAACAAATGA
SEQ ATGGAACAGGAATATTATCTGGGCTTGGACATGGGCACCGGTTCCGTCGGCTGGGCTGTT
ID ACTGACAGTGAATATCACGTTCTAAGAAAGCATGGTAAGGCATTGTGGGGTGTAAGACTT
NO: TTCGAATCTGCTTCCACTGCTGAAGAGCGTAGAATGTTTAGAACGAGTCGACGTAGGCTA
49 GACAGGCGCAATTGGAGAATCGAAATTTTACAAGAAATTTTTGCGGAAGAGATATCTAAG
AAAGACCCAGGCTTTTTCCTGAGAATGAAGGAATCTAAGTATTACCCTGAGGATAAAAGA
GATATAAATGGTAACTGTCCCGAATTGCCTTACGCATTATTTGTGGACGATGATTTTACC
GATAAGGATTACCATAAAAAGTTCCCAACTATCTACCATTTACGCAAAATGTTAATGAAT
ACAGAGGAAACCCCAGACATAAGACTAGTTTATCTGGCAATACACCATATGATGAAACAT
AGAGGCCATTTCTTACTTTCCGGGGATATCAACGAAATCAAAGAGTTTGGTACCACATTT
AGTAAGTTACTGGAAAACATAAAGAATGAAGAATTGGATTGGAACTTAGAACTCGGAAAA
GAAGAATACGCGGTTGTCGAATCTATCCTGAAGGATAATATGCTGAATAGGTCGACCAAA
AAAACTAGGCTGATCAAAGCACTGAAAGCCAAATCTATCTGCGAAAAAGCTGTTTTAAAT
TTACTTGCTGGTGGCACTGTTAAGTTATCAGACATTTTTGGTTTGGAAGAATTGAACGAA
ACCGAGCGTCCAAAAATTAGTTTCGCTGATAATGGCTACGATGATTACATTGGTGAGGTG
GAAAACGAGTTGGGCGAACAATTTTATATTATAGAGACAGCTAAGGCAGTCTATGACTGG
GCTGTTTTAGTAGAAATCCTTGGTAAATACACATCTATCTCCGAAGCGAAAGTTGCTACT
TACGAAAAGCACAAGTCCGATCTCCAGTTTTTGAAGAAAATTGTCAGGAAATATCTGACT
AAGGAAGAATATAAAGATATTTTCGTTAGTACCTCTGACAAACTGAAAAATTACTCCGCT
TACATCGGGATGACCAAGATTAATGGCAAAAAAGTTGATCTGCAAAGCAAAAGGTGTTCG
AAGGAAGAATTTTATGATTTCATTAAAAAGAATGTCTTAAAAAAATTAGAAGGTCAGCCA
GAATACGAATATTTGAAAGAAGAACTGGAAAGAGAGACATTCTTACCAAAACAAGTCAAC
AGAGATAATGGGGTAATTCCATATCAAATTCACCTCTACGAATTAAAAAAAATTTTAGGC
AATTTACGCGATAAAATTGACCTTATCAAAGAAAATGAGGATAAGCTGGTTCAACTCTTT
GAATTCAGAATACCCTATTATGTGGGCCCACTGAACAAGATTGATGACGGCAAAGAAGGT
AAATTCACATGGGCCGTCCGCAAATCCAATGAAAAAATTTACCCATGGAACTTTGAAAAT
GTAGTAGATATTGAAGCGTCTGCGGAGAAATTTATTCGAAGAATGACTAATAAATGCACT
TACTTGATGGGAGAGGATGTTCTGCCTAAAGACAGCTTATTATACAGCAAGTACATGGTT
CTAAACGAACTTAACAACGTTAAGTTGGACGGTGAGAAATTAAGTGTAGAATTGAAACAA
AGATTGTATACTGACGTCTTCTGCAAGTACAGAAAAGTGACAGTTAAAAAAATTAAGAAT
TACTTGAAGTGCGAAGGTATAATTTCTGGAAACGTAGAGATTACTGGTATTGATGGTGAT
TTCAAAGCATCCCTAACAGCTTACCACGATTTCAAGGAAATCCTGACAGGAACTGAACTC
GCAAAAAAAGATAAAGAAAACATTATTACTAATATTGTTCTTTTCGGTGATGACAAGAAA
TTGTTGAAGAAAAGACTGAATAGACTTTACCCCCAGATTACTCCCAATCAACTTAAGAAA
ATTTGTGCTTTGTCTTACACAGGATGGGGTCGTTTTTCAAAAAAGTTCTTAGAAGAGATT
ACCGCACCTGATCCAGAAACAGGCGAAGTATGGAATATAATTACCGCCTTATGGGAATCG
AACAATAATCTTATGCAACTTCTGAGCAATGAATATCGTTTCATGGAAGAAGTTGAGACT
TACAACATGGGCAAACAGACGAAGACTTTATCCTATGAAACTGTGGAAAATATGTATGTA
TCACCTTCTGTCAAGAGACAAATTTGGCAAACCTTAAAAATTGTCAAAGAATTAGAAAAG
GTAATGAAGGAGTCTCCTAAACGTGTGTTTATTGAAATGGCTAGAGAAAAACAAGAGTCA
AAAAGAACCGAGTCAAGAAAGAAGCAGTTAATCGATTTATATAAGGCTTGTAAAAACGAA
GAGAAAGATTGGGTTAAAGAATTGGGGGACCAAGAGGAACAAAAACTACGGTCGGATAAG
TTGTATTTATACTATACGCAAAAGGGACGATGTATGTATTCCGGCGAGGTAATAGAATTG
AAGGATTTATGGGACAATACAAAATATGACATAGACCATATATATCCCCAATCAAAAACG
ATGGACGATAGCTTGAACAATAGAGTACTCGTGAAAAAAAAATATAATGCGACCAAATCT
GATAAGTATCCTCTGAATGAAAATATCAGACATGAAAGAAAGGGGTTCTGGAAGTCCTTG
TTAGATGGTGGGTTTATAAGCAAAGAAAAGTACGAGCGTCTAATAAGAAACACGGAGTTA
TCGCCAGAAGAACTCGCTGGTTTTATTGAGAGGCAAATCGTGGAAACGAGACAATCTACC
AAAGCCGTTGCTGAGATCCTAAAGCAAGTTTTCCCAGAGTCGGAGATTGTCTATGTCAAA
GCTGGCACAGTGAGCAGGTTTAGGAAAGACTTCGAACTATTAAAGGTAAGAGAAGTGAAC
GATTTACATCACGCAAAGGACGCTTACCTAAATATCGTTGTAGGTAACTCATATTATGTT
AAATTTACCAAGAACGCCTCTTGGTTTATAAAGGAGAACCCAGGTAGAACATATAACCTG
AAAAAGATGTTCACCTCTGGTTGGAATATTGAGAGAAACGGAGAAGTCGCATGGGAAGTT
GGTAAGAAAGGGACTATAGTGACAGTAAAGCAAATTATGAACAAAAATAATATCCTCGTT
ACAAGGCAGGTTCATGAAGCAAAGGGCGGCCTTTTTGACCAACAAATTATGAAGAAAGGG
AAAGGTCAAATTGCAATAAAAGAAACCGATGAGAGACTAGCGTCAATAGAAAAGTATGGT
GGCTATAATAAAGCTGCGGGTGCATACTTTATGCTTGTTGAATCAAAAGACAAGAAAGGT
AAGACTATTAGAACTATAGAATTTATACCCCTGTACCTTAAAAACAAAATTGAATCGGAT
GAGTCAATCGCGTTAAATTTTCTAGAGAAAGGAAGGGGTTTAAAAGAACCAAAGATCCTG
TTAAAAAAGATTAAGATTGACACCTTGTTCGATGTAGATGGATTTAAAATGTGGTTATCT
GGCAGAACAGGCGATAGACTTTTGTTTAAGTGCGCTAATCAATTAATTTTGGATGAGAAA
ATCATTGTCACAATGAAAAAAATAGTTAAGTTTATTCAGAGAAGACAAGAAAACAGGGAG
TTGAAATTATCTGATAAAGATGGTATCGACAATGAAGTTTTAATGGAAATCTACAATACA
TTCGTTGATAAACTTGAAAATACCGTATATCGAATCAGGTTAAGTGAACAAGCCAAAACA
TTAATTGATAAACAAAAAGAATTTGAAAGGCTATCACTGGAAGACAAATCCTCCACCCTA
TTTGAAATTTTGCATATATTCCAGTGCCAATCTTCAGCAGCTAATTTAAAAATGATTGGC
GGACCTGGGAAAGCCGGCATCCTAGTGATGAACAATAATATCTCCAAGTGTAACAAAATA
TCAATTATTAACCAATCTCCGACAGGTATTTTTGAAAATGAAATAGACTTGCTTAAGATA
TAA
SEQ ATGTCTTTCGACTCTTTCACCAACCTGTACTCTCTGTCTAAAACCCTGAAATTCGAAATG
ID CGTCCGGTTGGTAACACCCAGAAAATGCTGGACAACGCGGGTGTTTTCGAAAAAGACAAA
NO: CTGATCCAGAAAAAATACGGTAAAACCAAACCGTACTTCGACCGTCTGCACCGTGAATTC
50 ATCGAAGAAGCGCTGACCGGTGTTGAACTGATCGGTCTGGACGAAAACTTCCGTACCCTG
GTTGACTGGCAGAAAGACAAAAAAAACAACGTTGCGATGAAAGCGTACGAAAACTCTCTG
CAGCGTCTGCGTACCGAAATCGGTAAAATCTTCAACCTGAAAGCGGAAGACTGGGTTAAA
AACAAATACCCGATCCTGGGTCTGAAAAACAAAAACACCGACATCCTGTTCGAAGAAGCG
GTTTTCGGTATCCTGAAAGCGCGTTACGGTGAAGAAAAAGACACCTTCATCGAAGTTGAA
GAAATCGACAAAACCGGTAAATCTAAAATCAACCAGATCTCTATCTTCGACTCTTGGAAA
GGTTTCACCGGTTACTTCAAAAAATTCTTCGAAACCCGTAAAAACTTCTACAAAAACGAC
GGTACCTCTACCGCGATCGCGACCCGTATCATCGACCAGAACCTGAAACGTTTCATCGAC
AACCTGTCTATCGTTGAATCTGTTCGTCAGAAAGTTGACCTGGCGGAAACCGAAAAATCT
TTCTCTATCTCTCTGTCTCAGTTCTTCTCTATCGACTTCTACAACAAATGCCTGCTGCAG
GACGGTATCGACTACTACAACAAAATCATCGGTGGTGAAACCCTGAAAAACGGTGAAAAA
CTGATCGGTCTGAACGAACTGATCAACCAGTACCGTCAGAACAACAAAGACCAGAAAATC
CCGTTCTTCAAACTGCTGGACAAACAGATCCTGTCTGAAAAAATCCTGTTCCTGGACGAA
ATCAAAAACGACACCGAACTGATCGAAGCGCTGTCTCAGTTCGCGAAAACCGCGGAAGAA
AAAACCAAAATCGTTAAAAAACTGTTCGCGGACTTCGTTGAAAACAACTCTAAATACGAC
CTGGCGCAGATCTACATCTCTCAGGAAGCGTTCAACACCATCTCTAACAAATGGACCTCT
GAAACCGAAACCTTCGCGAAATACCTGTTCGAAGCGATGAAATCTGGTAAACTGGCGAAA
TACGAAAAAAAAGACAACTCTTACAAATTCCCGGACTTCATCGCGCTGTCTCAGATGAAA
TCTGCGCTGCTGTCTATCTCTCTGGAAGGTCACTTCTGGAAAGAAAAATACTACAAAATC
TCTAAATTCCAGGAAAAAACCAACTGGGAACAGTTCCTGGCGATCTTCCTGTACGAATTC
AACTCTCTGTTCTCTGACAAAATCAACACCAAAGACGGTGAAACCAAACAGGTTGGTTAC
TACCTGTTCGCGAAAGACCTGCACAACCTGATCCTGTCTGAACAGATCGACATCCCGAAA
GACTCTAAAGTTACCATCAAAGACTTCGCGGACTCTGTTCTGACCATCTACCAGATGGCG
AAATACTTCGCGGTTGAAAAAAAACGTGCGTGGCTGGCGGAATACGAACTGGACTCTTTC
TACACCCAGCCGGACACCGGTTACCTGCAGTTCTACGACAACGCGTACGAAGACATCGTT
CAGGTTTACAACAAACTGCGTAACTACCTGACCAAAAAACCGTACTCTGAAGAAAAATGG
AAACTGAACTTCGAAAACTCTACCCTGGCGAACGGTTGGGACAAAAACAAAGAATCTGAC
AACTCTGCGGTTATCCTGCAGAAAGGTGGTAAATACTACCTGGGTCTGATCACCAAAGGT
CACAACAAAATCTTCGACGACCGTTTCCAGGAAAAATTCATCGTTGGTATCGAAGGTGGT
AAATACGAAAAAATCGTTTACAAATTCTTCCCGGACCAGGCGAAAATGTTCCCGAAAGTT
TGCTTCTCTGCGAAAGGTCTGGAATTCTTCCGTCCGTCTGAAGAAATCCTGCGTATCTAC
AACAACGCGGAATTCAAAAAAGGTGAAACCTACTCTATCGACTCTATGCAGAAACTGATC
GACTTCTACAAAGACTGCCTGACCAAATACGAAGGTTGGGCGTGCTACACCTTCCGTCAC
CTGAAACCGACCGAAGAATACCAGAACAACATCGGTGAATTCTTCCGTGACGTTGCGGAA
GACGGTTACCGTATCGACTTCCAGGGTATCTCTGACCAGTACATCCACGAAAAAAACGAA
AAAGGTGAACTGCACCTGTTCGAAATCCACAACAAAGACTGGAACCTGGACAAAGCGCGT
GACGGTAAATCTAAAACCACCCAGAAAAACCTGCACACCCTGTACTTCGAATCTCTGTTC
TCTAACGACAACGTTGTTCAGAACTTCCCGATCAAACTGAACGGTCAGGCGGAAATCTTC
TACCGTCCGAAAACCGAAAAAGACAAACTGGAATCTAAAAAAGACAAAAAAGGTAACAAA
GTTATCGACCACAAACGTTACTCTGAAAACAAAATCTTCTTCCACGTTCCGCTGACCCTG
AACCGTACCAAAAACGACTCTTACCGTTTCAACGCGCAGATCAACAACTTCCTGGCGAAC
AACAAAGACATCAACATCATCGGTGTTGACCGTGGTGAAAAACACCTGGTTTACTACTCT
GTTATCACCCAGGCGTCTGACATCCTGGAATCTGGTTCTCTGAACGAACTGAACGGTGTT
AACTACGCGGAAAAACTGGGTAAAAAAGCGGAAAACCGTGAACAGGCGCGTCGTGACTGG
CAGGACGTTCAGGGTATCAAAGACCTGAAAAAAGGTTACATCTCTCAGGTTGTTCGTAAA
CTGGCGGACCTGGCGATCAAACACAACGCGATCATCATCCTGGAAGACCTGAACATGCGT
TTCAAACAGGTTCGTGGTGGTATCGAAAAATCTATCTACCAGCAGCTGGAAAAAGCGCTG
ATCGACAAACTGTCTTTCCTGGTTGACAAAGGTGAAAAAAACCCGGAACAGGCGGGTCAC
CTGCTGAAAGCGTACCAGCTGTCTGCGCCGTTCGAAACCTTCCAGAAAATGGGTAAACAG
ACCGGTATCATCTTCTACACCCAGGCGTCTTACACCTCTAAATCTGACCCGGTTACCGGT
TGGCGTCCGCACCTGTACCTGAAATACTTCTCTGCGAAAAAAGCGAAAGACGACATCGCG
AAATTCACCAAAATCGAATTCGTTAACGACCGTTTCGAACTGACCTACGACATCAAAGAC
TTCCAGCAGGCGAAAGAATACCCGAACAAAACCGTTTGGAAAGTTTGCTCTAACGTTGAA
CGTTTCCGTTGGGACAAAAACCTGAACCAGAACAAAGGTGGTTACACCCACTACACCAAC
ATCACCGAAAACATCCAGGAACTGTTCACCAAATACGGTATCGACATCACCAAAGACCTG
CTGACCCAGATCTCTACCATCGACGAAAAACAGAACACCTCTTTCTTCCGTGACTTCATC
TTCTACTTCAACCTGATCTGCCAGATCCGTAACACCGACGACTCTGAAATCGCGAAAAAA
AACGGTAAAGACGACTTCATCCTGTCTCCGGTTGAACCGTTCTTCGACTCTCGTAAAGAC
AACGGTAACAAACTGCCGGAAAACGGTGACGACAACGGTGCGTACAACATCGCGCGTAAA
GGTATCGTTATCCTGAACAAAATCTCTCAGTACTCTGAAAAAAACGAAAACTGCGAAAAA
ATGAAATGGGGTGACCTGTACGTTTCTAACATCGACTGGGACAACTTCGTT
SEQ ATGGAAAACTTTAAAAACTTATACCCAATAAACAAAACGTTACGTTTTGAACTGCGTCCA
ID TATGGTAAAACACTGGAAAACTTTAAAAAAAGCGGTTTGTTGGAGAAGGATGCATTTAAA
NO: GCGAACTCTCGCAGATCCATGCAGGCCATCATTGATGAAAAATTTAAAGAGACGATCGAA
51 GAACGTCTGAAATACACGGAATTTAGTGAGTGTGACTTAGGTAATATGACTTCTAAAGAT
AAGAAAATCACCGATAAGGCGGCGACCAACCTGAAGAAGCAAGTCATTTTATCTTTTGAT
GATGAAATCTTTAACAACTATTTGAAACCGGACAAAAACATCGATGCCTTATTTAAAAAT
GACCCTTCGAACCCGGTGATTAGCACATTTAAGGGCTTCACAACGTATTTTGTCAATTTT
TTTGAAATTCGTAAACATATCTTCAAAGGAGAATCAAGCGGCTCTATGGCTTATCGCATT
ATTGATGAAAACCTGACGACCTATTTGAATAACATTGAAAAAATCAAAAAACTGCCAGAG
GAATTAAAGTCTCAGTTAGAAGGCATCGACCAGATCGACAAACTCAACAACTATAACGAA
TTTATTACGCAGTCTGGTATCACCCACTATAATGAAATTATTGGAGGTATCAGTAAATCA
GAAAATGTGAAAATCCAAGGGATTAATGAAGGCATTAACCTCTATTGCCAGAAAAATAAA
GTGAAACTGCCGAGGCTGACTCCACTCTACAAAATGATCCTGTCTGACCGCGTCTCGAAT
AGCTTTGTCCTGGACACAATTGAAAACGATACGGAATTGATTGAGATGATAAGCGATCTG
ATTAACAAAACCGAAATTTCACAGGATGTAATCATGAGTGATATACAAAACATCTTTATT
AAATATAAACAGCTTGGTAATCTGCCTGGAATTAGCTATTCGTCAATAGTGAACGCAATC
TGTTCTGATTATGATAACAATTTTGGCGACGGTAAGCGTAAAAAGAGTTATGAAAACGAT
AGGAAAAAACACCTGGAAACTAACGTGTATTCTATCAACTATATCAGCGAACTGCTTACG
GACACCGATGTGAGTTCAAACATTAAGATGCGGTATAAGGAGCTTGAACAGAACTACCAG
GTCTGTAAGGAAAACTTCAACGCAACCAACTGGATGAACATTAAAAATATCAAACAATCC
GAGAAGACCAACTTAATCAAAGATCTGCTGGATATTTTGAAGAGCATTCAACGTTTTTAT
GATCTGTTCGATATCGTTGATGAAGACAAGAATCCTAGTGCGGAATTTTATACATGGCTG
TCTAAAAATGCGGAGAAATTGGATTTCGAATTCAATTCTGTTTATAATAAATCACGCAAC
TATTTGACCCGCAAACAATACAGCGACAAAAAGATAAAACTAAACTTCGACAGTCCGACA
TTGGCAAAGGGCTGGGACGCAAATAAGGAAATCGATAACTCTACGATAATTATGCGTAAG
TTCAATAATGATCGAGGTGATTATGATTATTTCTTAGGCATTTGGAACAAAAGCACCCCG
GCCAACGAAAAGATAATTCCACTGGAGGATAACGGTCTGTTCGAAAAAATGCAGTACAAA
TTATATCCGGATCCAAGCAAGATGCTTCCAAAGCAGTTTCTGTCTAAAATTTGGAAAGCT
AAGCATCCGACCACCCCAGAATTTGACAAGAAATATAAGGAAGGCCGCCATAAGAAAGGT
CCCGATTTTGAAAAAGAATTCTTGCACGAACTGATTGATTGCTTTAAACATGGCTTAGTC
AATCACGATGAAAAGTATCAAGATGTTTTTGGATTCAATTTGAGAAACACAGAAGACTAC
AATTCCTACACTGAGTTTCTCGAAGATGTGGAACGATGTAATTATAATCTGAGCTTTAAC
AAAATCGCGGACACCTCGAATCTGATTAACGATGGTAAACTTTATGTTTTCCAGATCTGG
AGCAAGGATTTCTCTATTGACAGCAAAGGCACCAAAAACCTGAACACCATTTACTTTGAA
AGTCTCTTCAGCGAAGAAAATATGATTGAGAAAATGTTTAAACTTAGCGGTGAAGCTGAA
ATATTCTATCGCCCGGCAAGCCTGAACTATTGCGAAGACATTATCAAAAAGGGTCATCAC
CACGCTGAACTGAAAGATAAATTTGATTATCCTATCATAAAAGATAAACGCTATAGCCAG
GATAAATTTTTTTTTCATGTTCCTATGGTCATTAACTACAAATCAGAAAAACTGAACTCT
AAAAGCCTCAATAATCGAACCAATGAAAACCTTGGGCAGTTTACCCATATAATTGGAATT
GATCGCGGAGAGCGTCATTTAATCTACCTGACCGTAGTCGATGTATCGACCGGCGAGATC
GTCGAGCAGAAGCACTTAGACGAGATTATCAACACTGATACCAAAGGTGTTGAGCATAAG
ACGCACTATCTAAACAAGCTGGAGGAAAAATCGAAAACCCGTGATAATGAACGTAAGAGT
TGGGAGGCAATTGAAACGATTAAAGAACTGAAGGAGGGTTATATCAGCCACGTAATCAAT
GAAATTCAAAAACTGCAGGAAAAATACAACGCCCTGATCGTTATGGAAAATCTGAATTAC
GGTTTCAAAAATTCTCGCATCAAAGTGGAAAAACAGGTATATCAGAAGTTCGAGACGGCA
TTAATTAAAAAGTTTAATTACATCATTGACAAAAAAGATCCGGAAACTTATATTCATGGC
TATCAGCTGACGAACCCGATCACCACACTGGATAAAATTGGTAACCAGTCTGGTATCGTG
CTTTACATCCCTGCCTGGAATACCAGTAAAATCGATCCGGTAACGGGATTCGTCAACCTT
CTATATGCAGATGACCTCAAATATAAGAATCAGGAACAGGCCAAGTCTTTTATTCAGAAA
ATCGATAACATTTACTTTGAGAATGGGGAATTCAAATTTGATATTGATTTTTCTAAATGG
AACAATCGTTATAGTATATCTAAGACGAAATGGACGCTCACCTCGTACGGAACCCGAATC
CAGACATTCCGCAATCCGCAGAAGAACAATAAATGGGACAGCGCCGAGTATGATCTCACT
GAAGAATTCAAATTGATTCTGAACATTGACGGTACCCTGAAAAGCCAGGATGTCGAAACC
TATAAAAAATTTATGTCTCTGTTCAAGCTGATGCTGCAACTTAGGAACTCTGTTACCGGC
ACTGATATCGATTATATGATCTCCCCTGTCACTGATAAAACAGGTACGCATTTCGATTCG
CGCGAAAATATCAAAAATCTGCCCGCAGATGCCGACGCCAATGGGGCGTACAATATTGCA
CGCAAGGGTATCATGGCGATCGAAAACATTATGAATGGTATCAGCGACCCGCTGAAAATC
TCAAACGAAGATTATTTGAAATATATCCAAAACCAGCAGGAATAA
SEQ ATGACCCAGTTCGAAGGTTTCACCAACCTGTACCAGGTTTCTAAAACCCTGCGTTTCGAA
ID CTGATCCCGCAGGGTAAAACCCTGAAACACATCCAGGAACAGGGTTTCATCGAAGAAGAC
NO: AAAGCGCGTAACGACCACTACAAAGAACTGAAACCGATCATCGACCGTATCTACAAAACC
52 TACGCGGACCAGTGCCTGCAGCTGGTTCAGCTGGACTGGGAAAACCTGTCTGCGGCGATC
GACTCTTACCGTAAAGAAAAAACCGAAGAAACCCGTAACGCGCTGATCGAAGAACAGGCG
ACCTACCGTAACGCGATCCACGACTACTTCATCGGTCGTACCGACAACCTGACCGACGCG
ATCAACAAACGTCACGCGGAAATCTACAAAGGTCTGTTCAAAGCGGAACTGTTCAACGGT
AAAGTTCTGAAACAGCTGGGTACCGTTACCACCACCGAACACGAAAACGCGCTGCTGCGT
TCTTTCGACAAATTCACCACCTACTTCTCTGGTTTCTACGAAAACCGTAAAAACGTTTTC
TCTGCGGAAGACATCTCTACCGCGATCCCGCACCGTATCGTTCAGGACAACTTCCCGAAA
TTCAAAGAAAACTGCCACATCTTCACCCGTCTGATCACCGCGGTTCCGTCTCTGCGTGAA
CACTTCGAAAACGTTAAAAAAGCGATCGGTATCTTCGTTTCTACCTCTATCGAAGAAGTT
TTCTCTTTCCCGTTCTACAACCAGCTGCTGACCCAGACCCAGATCGACCTGTACAACCAG
CTGCTGGGTGGTATCTCTCGTGAAGCGGGTACCGAAAAAATCAAAGGTCTGAACGAAGTT
CTGAACCTGGCGATCCAGAAAAACGACGAAACCGCGCACATCATCGCGTCTCTGCCGCAC
CGTTTCATCCCGCTGTTCAAACAGATCCTGTCTGACCGTAACACCCTGTCTTTCATCCTG
GAAGAATTCAAATCTGACGAAGAAGTTATCCAGTCTTTCTGCAAATACAAAACCCTGCTG
CGTAACGAAAACGTTCTGGAAACCGCGGAAGCGCTGTTCAACGAACTGAACTCTATCGAC
CTGACCCACATCTTCATCTCTCACAAAAAACTGGAAACCATCTCTTCTGCGCTGTGCGAC
CACTGGGACACCCTGCGTAACGCGCTGTACGAACGTCGTATCTCTGAACTGACCGGTAAA
ATCACCAAATCTGCGAAAGAAAAAGTTCAGCGTTCTCTGAAACACGAAGACATCAACCTG
CAGGAAATCATCTCTGCGGCGGGTAAAGAACTGTCTGAAGCGTTCAAACAGAAAACCTCT
GAAATCCTGTCTCACGCGCACGCGGCGCTGGACCAGCCGCTGCCGACCACCCTGAAAAAA
CAGGAAGAAAAAGAAATCCTGAAATCTCAGCTGGACTCTCTGCTGGGTCTGTACCACCTG
CTGGACTGGTTCGCGGTTGACGAATCTAACGAAGTTGACCCGGAATTCTCTGCGCGTCTG
ACCGGTATCAAACTGGAAATGGAACCGTCTCTGTCTTTCTACAACAAAGCGCGTAACTAC
GCGACCAAAAAACCGTACTCTGTTGAAAAATTCAAACTGAACTTCCAGATGCCGACCCTG
GCGTCTGGTTGGGACGTTAACAAAGAAAAAAACAACGGTGCGATCCTGTTCGTTAAAAAC
GGTCTGTACTACCTGGGTATCATGCCGAAACAGAAAGGTCGTTACAAAGCGCTGTCTTTC
GAACCGACCGAAAAAACCTCTGAAGGTTTCGACAAAATGTACTACGACTACTTCCCGGAC
GCGGCGAAAATGATCCCGAAATGCTCTACCCAGCTGAAAGCGGTTACCGCGCACTTCCAG
ACCCACACCACCCCGATCCTGCTGTCTAACAACTTCATCGAACCGCTGGAAATCACCAAA
GAAATCTACGACCTGAACAACCCGGAAAAAGAACCGAAAAAATTCCAGACCGCGTACGCG
AAAAAAACCGGTGACCAGAAAGGTTACCGTGAAGCGCTGTGCAAATGGATCGACTTCACC
CGTGACTTCCTGTCTAAATACACCAAAACCACCTCTATCGACCTGTCTTCTCTGCGTCCG
TCTTCTCAGTACAAAGACCTGGGTGAATACTACGCGGAACTGAACCCGCTGCTGTACCAC
ATCTCTTTCCAGCGTATCGCGGAAAAAGAAATCATGGACGCGGTTGAAACCGGTAAACTG
TACCTGTTCCAGATCTACAACAAAGACTTCGCGAAAGGTCACCACGGTAAACCGAACCTG
CACACCCTGTACTGGACCGGTCTGTTCTCTCCGGAAAACCTGGCGAAAACCTCTATCAAA
CTGAACGGTCAGGCGGAACTGTTCTACCGTCCGAAATCTCGTATGAAACGTATGGCGCAC
CGTCTGGGTGAAAAAATGCTGAACAAAAAACTGAAAGACCAGAAAACCCCGATCCCGGAC
ACCCTGTACCAGGAACTGTACGACTACGTTAACCACCGTCTGTCTCACGACCTGTCTGAC
GAAGCGCGTGCGCTGCTGCCGAACGTTATCACCAAAGAAGTTTCTCACGAAATCATCAAA
GACCGTCGTTTCACCTCTGACAAATTCTTCTTCCACGTTCCGATCACCCTGAACTACCAG
GCGGCGAACTCTCCGTCTAAATTCAACCAGCGTGTTAACGCGTACCTGAAAGAACACCCG
GAAACCCCGATCATCGGTATCGACCGTGGTGAACGTAACCTGATCTACATCACCGTTATC
GACTCTACCGGTAAAATCCTGGAACAGCGTTCTCTGAACACCATCCAGCAGTTCGACTAC
CAGAAAAAACTGGACAACCGTGAAAAAGAACGTGTTGCGGCGCGTCAGGCGTGGTCTGTT
GTTGGTACCATCAAAGACCTGAAACAGGGTTACCTGTCTCAGGTTATCCACGAAATCGTT
GACCTGATGATCCACTACCAGGCGGTTGTTGTTCTGGAAAACCTGAACTTCGGTTTCAAA
TCTAAACGTACCGGTATCGCGGAAAAAGCGGTTTACCAGCAGTTCGAAAAAATGCTGATC
GACAAACTGAACTGCCTGGTTCTGAAAGACTACCCGGCGGAAAAAGTTGGTGGTGTTCTG
AACCCGTACCAGCTGACCGACCAGTTCACCTCTTTCGCGAAAATGGGTACCCAGTCTGGT
TTCCTGTTCTACGTTCCGGCGCCGTACACCTCTAAAATCGACCCGCTGACCGGTTTCGTT
GACCCGTTCGTTTGGAAAACCATCAAAAACCACGAATCTCGTAAACACTTCCTGGAAGGT
TTCGACTTCCTGCACTACGACGTTAAAACCGGTGACTTCATCCTGCACTTCAAAATGAAC
CGTAACCTGTCTTTCCAGCGTGGTCTGCCGGGTTTCATGCCGGCGTGGGACATCGTTTTC
GAAAAAAACGAAACCCAGTTCGACGCGAAAGGTACCCCGTTCATCGCGGGTAAACGTATC
GTTCCGGTTATCGAAAACCACCGTTTCACCGGTCGTTACCGTGACCTGTACCCGGCGAAC
GAACTGATCGCGCTGCTGGAAGAAAAAGGTATCGTTTTCCGTGACGGTTCTAACATCCTG
CCGAAACTGCTGGAAAACGACGACTCTCACGCGATCGACACCATGGTTGCGCTGATCCGT
TCTGTTCTGCAGATGCGTAACTCTAACGCGGCGACCGGTGAAGACTACATCAACTCTCCG
GTTCGTGACCTGAACGGTGTTTGCTTCGACTCTCGTTTCCAGAACCCGGAATGGCCGATG
GACGCGGACGCGAACGGTGCGTACCACATCGCGCTGAAAGGTCAGCTGCTGCTGAACCAC
CTGAAAGAATCTAAAGACCTGAAACTGCAGAACGGTATCTCTAACCAGGACTGGCTGGCG
TACATCCAGGAACTGCGTAACTA
SEQ ATGGCGGTTAAATCTATCAAAGTTAAACTGCGTCTGGACGACATGCCGGAAATCCGTGCG
ID GGTCTGTGGAAACTGCACAAAGAAGTTAACGCGGGTGTTCGTTACTACACCGAATGGCTG
NO: TCTCTGCTGCGTCAGGAAAACCTGTACCGTCGTTCTCCGAACGGTGACGGTGAACAGGAA
53 TGCGACAAAACCGCGGAAGAATGCAAAGCGGAACTGCTGGAACGTCTGCGTGCGCGTCAG
GTTGAAAACGGTCACCGTGGTCCGGCGGGTTCTGACGACGAACTGCTGCAGCTGGCGCGT
CAGCTGTACGAACTGCTGGTTCCGCAGGCGATCGGTGCGAAAGGTGACGCGCAGCAGATC
GCGCGTAAATTCCTGTCTCCGCTGGCGGACAAAGACGCGGTTGGTGGTCTGGGTATCGCG
AAAGCGGGTAACAAACCGCGTTGGGTTCGTATGCGTGAAGCGGGTGAACCGGGTTGGGAA
GAAGAAAAAGAAAAAGCGGAAACCCGTAAATCTGCGGACCGTACCGCGGACGTTCTGCGT
GCGCTGGCGGACTTCGGTCTGAAACCGCTGATGCGTGTTTACACCGACTCTGAAATGTCT
TCTGTTGAATGGAAACCGCTGCGTAAAGGTCAGGCGGTTCGTACCTGGGACCGTGACATG
TTCCAGCAGGCGATCGAACGTATGATGTCTTGGGAATCTTGGAACCAGCGTGTTGGTCAG
GAATACGCGAAACTGGTTGAACAGAAAAACCGTTTCGAACAGAAAAACTTCGTTGGTCAG
GAACACCTGGTTCACCTGGTTAACCAGCTGCAGCAGGACATGAAAGAAGCGTCTCCGGGT
CTGGAATCTAAAGAACAGACCGCGCACTACGTTACCGGTCGTGCGCTGCGTGGTTCTGAC
AAAGTTTTCGAAAAATGGGGTAAACTGGCGCCGGACGCGCCGTTCGACCTGTACGACGCG
GAAATCAAAAACGTTCAGCGTCGTAACACCCGTCGTTTCGGTTCTCACGACCTGTTCGCG
AAACTGGCGGAACCGGAATACCAGGCGCTGTGGCGTGAAGACGCGTCTTTCCTGACCCGT
TACGCGGTTTACAACTCTATCCTGCGTAAACTGAACCACGCGAAAATGTTCGCGACCTTC
ACCCTGCCGGACGCGACCGCGCACCCGATCTGGACCCGTTTCGACAAACTGGGTGGTAAC
CTGCACCAGTACACCTTCCTGTTCAACGAATTCGGTGAACGTCGTCACGCGATCCGTTTC
CACAAACTGCTGAAAGTTGAAAACGGTGTTGCGCGTGAAGTTGACGACGTTACCGTTCCG
ATCTCTATGTCTGAACAGCTGGACAACCTGCTGCCGCGTGACCCGAACGAACCGATCGCG
CTGTACTTCCGTGACTACGGTGCGGAACAGCACTTCACCGGTGAATTCGGTGGTGCGAAA
ATCCAGTGCCGTCGTGACCAGCTGGCGCACATGCACCGTCGTCGTGGTGCGCGTGACGTT
TACCTGAACGTTTCTGTTCGTGTTCAGTCTCAGTCTGAAGCGCGTGGTGAACGTCGTCCG
CCGTACGCGGCGGTTTTCCGTCTGGTTGGTGACAACCACCGTGCGTTCGTTCACTTCGAC
AAACTGTCTGACTACCTGGCGGAACACCCGGACGACGGTAAACTGGGTTCTGAAGGTCTG
CTGTCTGGTCTGCGTGTTATGTCTGTTGACCTGGGTCTGCGTACCTCTGCGTCTATCTCT
GTTTTCCGTGTTGCGCGTAAAGACGAACTGAAACCGAACTCTAAAGGTCGTGTTCCGTTC
TTCTTCCCGATCAAAGGTAACGACAACCTGGTTGCGGTTCACGAACGTTCTCAGCTGCTG
AAACTGCCGGGTGAAACCGAATCTAAAGACCTGCGTGCGATCCGTGAAGAACGTCAGCGT
ACCCTGCGTCAGCTGCGTACCCAGCTGGCGTACCTGCGTCTGCTGGTTCGTTGCGGTTCT
GAAGACGTTGGTCGTCGTGAACGTTCTTGGGCGAAACTGATCGAACAGCCGGTTGACGCG
GCGAACCACATGACCCCGGACTGGCGTGAAGCGTTCGAAAACGAACTGCAGAAACTGAAA
TCTCTGCACGGTATCTGCTCTGACAAAGAATGGATGGACGCGGTTTACGAATCTGTTCGT
CGTGTTTGGCGTCACATGGGTAAACAGGTTCGTGACTGGCGTAAAGACGTTCGTTCTGGT
GAACGTCCGAAAATCCGTGGTTACGCGAAAGACGTTGTTGGTGGTAACTCTATCGAACAG
ATCGAATACCTGGAACGTCAGTACAAATTCCTGAAATCTTGGTCTTTCTTCGGTAAAGTT
TCTGGTCAGGTTATCCGTGCGGAAAAAGGTTCTCGTTTCGCGATCACCCTGCGTGAACAC
ATCGACCACGCGAAAGAAGACCGTCTGAAAAAACTGGCGGACCGTATCATCATGGAAGCG
CTGGGTTACGTTTACGCGCTGGACGAACGTGGTAAAGGTAAATGGGTTGCGAAATACCCG
CCGTGCCAGCTGATCCTGCTGGAAGAACTGTCTGAATACCAGTTCAACAACGACCGTCCG
CCGTCTGAAAACAACCAGCTGATGCAGTGGTCTCACCGTGGTGTTTTCCAGGAACTGATC
AACCAGGCGCAGGTTCACGACCTGCTGGTTGGTACCATGTACGCGGCGTTCTCTTCTCGT
TTCGACGCGCGTACCGGTGCGCCGGGTATCCGTTGCCGTCGTGTTCCGGCGCGTTGCACC
CAGGAACACAACCCGGAACCGTTCCCGTGGTGGCTGAACAAATTCGTTGTTGAACACACC
CTGGACGCGTGCCCGCTGCGTGCGGACGACCTGATCCCGACCGGTGAAGGTGAAATCTTC
GTTTCTCCGTTCTCTGCGGAAGAAGGTGACTTCCACCAGATCCACGCGGACCTGAACGCG
GCGCAGAACCTGCAGCAGCGTCTGTGGTCTGACTTCGACATCTCTCAGATCCGTCTGCGT
TGCGACTGGGGTGAAGTTGACGGTGAACTGGTTCTGATCCCGCGTCTGACCGGTAAACGT
ACCGCGGACTCTTACTCTAACAAAGTTTTCTACACCAACACCGGTGTTACCTACTACGAA
CGTGAACGTGGTAAAAAACGTCGTAAAGTTTTCGCGCAGGAAAAACTGTCTGAAGAAGAA
GCGGAACTGCTGGTTGAAGCGGACGAAGCGCGTGAAAAATCTGTTGTTCTGATGCGTGAC
CCGTCTGGTATCATCAACCGTGGTAACTGGACCCGTCAGAAAGAATTCTGGTCTATGGTT
AACCAGCGTATCGAAGGTTACCTGGTTAAACAGATCCGTTCTCGTGTTCCGCTGCAGGAC
TCTGCGTGCGAAAACACCGGTGACATCTAA
SEQ ATGGCGACCCGTTCTTTCATCCTGAAAATCGAACCGAACGAAGAAGTTAAAAAAGGTCTG
ID TGGAAAACCCACGAAGTTCTGAACCACGGTATCGCGTACTACATGAACATCCTGAAACTG
NO: ATCCGTCAGGAAGCGATCTACGAACACCACGAACAGGACCCGAAAAACCCGAAAAAAGTT
54 TCTAAAGCGGAAATCCAGGCGGAACTGTGGGACTTCGTTCTGAAAATGCAGAAATGCAAC
TCTTTCACCCACGAAGTTGACAAAGACGTTGTTTTCAACATCCTGCGTGAACTGTACGAA
GAACTGGTTCCGTCTTCTGTTGAAAAAAAAGGTGAAGCGAACCAGCTGTCTAACAAATTC
CTGTACCCGCTGGTTGACCCGAACTCTCAGTCTGGTAAAGGTACCGCGTCTTCTGGTCGT
AAACCGCGTTGGTACAACCTGAAAATCGCGGGTGACCCGTCTTGGGAAGAAGAAAAAAAA
AAATGGGAAGAAGACAAAAAAAAAGACCCGCTGGCGAAAATCCTGGGTAAACTGGCGGAA
TACGGTCTGATCCCGCTGTTCATCCCGTTCACCGACTCTAACGAACCGATCGTTAAAGAA
ATCAAATGGATGGAAAAATCTCGTAACCAGTCTGTTCGTCGTCTGGACAAAGACATGTTC
ATCCAGGCGCTGGAACGTTTCCTGTCTTGGGAATCTTGGAACCTGAAAGTTAAAGAAGAA
TACGAAAAAGTTGAAAAAGAACACAAAACCCTGGAAGAACGTATCAAAGAAGACATCCAG
GCGTTCAAATCTCTGGAACAGTACGAAAAAGAACGTCAGGAACAGCTGCTGCGTGACACC
CTGAACACCAACGAATACCGTCTGTCTAAACGTGGTCTGCGTGGTTGGCGTGAAATCATC
CAGAAATGGCTGAAAATGGACGAAAACGAACCGTCTGAAAAATACCTGGAAGTTTTCAAA
GACTACCAGCGTAAACACCCGCGTGAAGCGGGTGACTACTCTGTTTACGAATTCCTGTCT
AAAAAAGAAAACCACTTCATCTGGCGTAACCACCCGGAATACCCGTACCTGTACGCGACC
TTCTGCGAAATCGACAAAAAAAAAAAAGACGCGAAACAGCAGGCGACCTTCACCCTGGCG
GACCCGATCAACCACCCGCTGTGGGTTCGTTTCGAAGAACGTTCTGGTTCTAACCTGAAC
AAATACCGTATCCTGACCGAACAGCTGCACACCGAAAAACTGAAAAAAAAACTGACCGTT
CAGCTGGACCGTCTGATCTACCCGACCGAATCTGGTGGTTGGGAAGAAAAAGGTAAAGTT
GACATCGTTCTGCTGCCGTCTCGTCAGTTCTACAACCAGATCTTCCTGGACATCGAAGAA
AAAGGTAAACACGCGTTCACCTACAAAGACGAATCTATCAAATTCCCGCTGAAAGGTACC
CTGGGTGGTGCGCGTGTTCAGTTCGACCGTGACCACCTGCGTCGTTACCCGCACAAAGTT
GAATCTGGTAACGTTGGTCGTATCTACTTCAACATGACCGTTAACATCGAACCGACCGAA
TCTCCGGTTTCTAAATCTCTGAAAATCCACCGTGACGACTTCCCGAAATTCGTTAACTTC
AAACCGAAAGAACTGACCGAATGGATCAAAGACTCTAAAGGTAAAAAACTGAAATCTGGT
ATCGAATCTCTGGAAATCGGTCTGCGTGTTATGTCTATCGACCTGGGTCAGCGTCAGGCG
GCGGCGGCGTCTATCTTCGAAGTTGTTGACCAGAAACCGGACATCGAAGGTAAACTGTTC
TTCCCGATCAAAGGTACCGAACTGTACGCGGTTCACCGTGCGTCTTTCAACATCAAACTG
CCGGGTGAAACCCTGGTTAAATCTCGTGAAGTTCTGCGTAAAGCGCGTGAAGACAACCTG
AAACTGATGAACCAGAAACTGAACTTCCTGCGTAACGTTCTGCACTTCCAGCAGTTCGAA
GACATCACCGAACGTGAAAAACGTGTTACCAAATGGATCTCTCGTCAGGAAAACTCTGAC
GTTCCGCTGGTTTACCAGGACGAACTGATCCAGATCCGTGAACTGATGTACAAACCGTAC
AAAGACTGGGTTGCGTTCCTGAAACAGCTGCACAAACGTCTGGAAGTTGAAATCGGTAAA
GAAGTTAAACACTGGCGTAAATCTCTGTCTGACGGTCGTAAAGGTCTGTACGGTATCTCT
CTGAAAAACATCGACGAAATCGACCGTACCCGTAAATTCCTGCTGCGTTGGTCTCTGCGT
CCGACCGAACCGGGTGAAGTTCGTCGTCTGGAACCGGGTCAGCGTTTCGCGATCGACCAG
CTGAACCACCTGAACGCGCTGAAAGAAGACCGTCTGAAAAAAATGGCGAACACCATCATC
ATGCACGCGCTGGGTTACTGCTACGACGTTCGTAAAAAAAAATGGCAGGCGAAAAACCCG
GCGTGCCAGATCATCCTGTTCGAAGACCTGTCTAACTACAACCCGTACGAAGAACGTTCT
CGTTTCGAAAACTCTAAACTGATGAAATGGTCTCGTCGTGAAATCCCGCGTCAGGTTGCG
CTGCAGGGTGAAATCTACGGTCTGCAGGTTGGTGAAGTTGGTGCGCAGTTCTCTTCTCGT
TTCCACGCGAAAACCGGTTCTCCGGGTATCCGTTGCTCTGTTGTTACCAAAGAAAAACTG
CAGGACAACCGTTTCTTCAAAAACCTGCAGCGTGAAGGTCGTCTGACCCTGGACAAAATC
GCGGTTCTGAAAGAAGGTGACCTGTACCCGGACAAAGGTGGTGAAAAATTCATCTCTCTG
TCTAAAGACCGTAAACTGGTTACCACCCACGCGGACATCAACGCGGCGCAGAACCTGCAG
AAACGTTTCTGGACCCGTACCCACGGTTTCTACAAAGTTTACTGCAAAGCGTACCAGGTT
GACGGTCAGACCGTTTACATCCCGGAATCTAAAGACCAGAAACAGAAAATCATCGAAGAA
TTCGGTGAAGGTTACTTCATCCTGAAAGACGGTGTTTACGAATGGGGTAACGCGGGTAAA
CTGAAAATCAAAAAAGGTTCTTCTAAACAGTCTTCTTCTGAACTGGTTGACTCTGACATC
CTGAAAGACTCTTTCGACCTGGCGTCTGAACTGAAAGGTGAAAAACTGATGCTGTACCGT
GACCCGTCTGGTAACGTTTTCCCGTCTGACAAATGGATGGCGGCGGGTGTTTTCTTCGGT
AAACTGGAACGTATCCTGATCTCTAAACTGACCAACCAGTACTCTATCTCTACCATCGAA
GACGACTCTTCTAAACAGTCTATGTAA
SEQ ATGCCGACCCGTACCATCAACCTGAAACTGGTTCTGGGTAAAAACCCGGAAAACGCGACC
ID CTGCGTCGTGCGCTGTTCTCTACCCACCGTCTGGTTAACCAGGCGACCAAACGTATCGAA
NO: GAATTCCTGCTGCTGTGCCGTGGTGAAGCGTACCGTACCGTTGACAACGAAGGTAAAGAA
55 GCGGAAATCCCGCGTCACGCGGTTCAGGAAGAAGCGCTGGCGTTCGCGAAAGCGGCGCAG
CGTCACAACGGTTGCATCTCTACCTACGAAGACCAGGAAATCCTGGACGTTCTGCGTCAG
CTGTACGAACGTCTGGTTCCGTCTGTTAACGAAAACAACGAAGCGGGTGACGCGCAGGCG
GCGAACGCGTGGGTTTCTCCGCTGATGTCTGCGGAATCTGAAGGTGGTCTGTCTGTTTAC
GACAAAGTTCTGGACCCGCCGCCGGTTTGGATGAAACTGAAAGAAGAAAAAGCGCCGGGT
TGGGAAGCGGCGTCTCAGATCTGGATCCAGTCTGACGAAGGTCAGTCTCTGCTGAACAAA
CCGGGTTCTCCGCCGCGTTGGATCCGTAAACTGCGTTCTGGTCAGCCGTGGCAGGACGAC
TTCGTTTCTGACCAGAAAAAAAAACAGGACGAACTGACCAAAGGTAACGCGCCGCTGATC
AAACAGCTGAAAGAAATGGGTCTGCTGCCGCTGGTTAACCCGTTCTTCCGTCACCTGCTG
GACCCGGAAGGTAAAGGTGTTTCTCCGTGGGACCGTCTGGCGGTTCGTGCGGCGGTTGCG
CACTTCATCTCTTGGGAATCTTGGAACCACCGTACCCGTGCGGAATACAACTCTCTGAAA
CTGCGTCGTGACGAATTCGAAGCGGCGTCTGACGAATTCAAAGACGACTTCACCCTGCTG
CGTCAGTACGAAGCGAAACGTCACTCTACCCTGAAATCTATCGCGCTGGCGGACGACTCT
AACCCGTACCGTATCGGTGTTCGTTCTCTGCGTGCGTGGAACCGTGTTCGTGAAGAATGG
ATCGACAAAGGTGCGACCGAAGAACAGCGTGTTACCATCCTGTCTAAACTGCAGACCCAG
CTGCGTGGTAAATTCGGTGACCCGGACCTGTTCAACTGGCTGGCGCAGGACCGTCACGTT
CACCTGTGGTCTCCGCGTGACTCTGTTACCCCGCTGGTTCGTATCAACGCGGTTGACAAA
GTTCTGCGTCGTCGTAAACCGTACGCGCTGATGACCTTCGCGCACCCGCGTTTCCACCCG
CGTTGGATCCTGTACGAAGCGCCGGGTGGTTCTAACCTGCGTCAGTACGCGCTGGACTGC
ACCGAAAACGCGCTGCACATCACCCTGCCGCTGCTGGTTGACGACGCGCACGGTACCTGG
ATCGAAAAAAAAATCCGTGTTCCGCTGGCGCCGTCTGGTCAGATCCAGGACCTGACCCTG
GAAAAACTGGAAAAAAAAAAAAACCGTCTGTACTACCGTTCTGGTTTCCAGCAGTTCGCG
GGTCTGGCGGGTGGTGCGGAAGTTCTGTTCCACCGTCCGTACATGGAACACGACGAACGT
TCTGAAGAATCTCTGCTGGAACGTCCGGGTGCGGTTTGGTTCAAACTGACCCTGGACGTT
GCGACCCAGGCGCCGCCGAACTGGCTGGACGGTAAAGGTCGTGTTCGTACCCCGCCGGAA
GTTCACCACTTCAAAACCGCGCTGTCTAACAAATCTAAACACACCCGTACCCTGCAGCCG
GGTCTGCGTGTTCTGTCTGTTGACCTGGGTATGCGTACCTTCGCGTCTTGCTCTGTTTTC
GAACTGATCGAAGGTAAACCGGAAACCGGTCGTGCGTTCCCGGTTGCGGACGAACGTTCT
ATGGACTCTCCGAACAAACTGTGGGCGAAACACGAACGTTCTTTCAAACTGACCCTGCCG
GGTGAAACCCCGTCTCGTAAAGAAGAAGAAGAACGTTCTATCGCGCGTGCGGAAATCTAC
GCGCTGAAACGTGACATCCAGCGTCTGAAATCTCTGCTGCGTCTGGGTGAAGAAGACAAC
GACAACCGTCGTGACGCGCTGCTGGAACAGTTCTTCAAAGGTTGGGGTGAAGAAGACGTT
GTTCCGGGTCAGGCGTTCCCGCGTTCTCTGTTCCAGGGTCTGGGTGCGGCGCCGTTCCGT
TCTACCCCGGAACTGTGGCGTCAGCACTGCCAGACCTACTACGACAAAGCGGAAGCGTGC
CTGGCGAAACACATCTCTGACTGGCGTAAACGTACCCGTCCGCGTCCGACCTCTCGTGAA
ATGTGGTACAAAACCCGTTCTTACCACGGTGGTAAATCTATCTGGATGCTGGAATACCTG
GACGCGGTTCGTAAACTGCTGCTGTCTTGGTCTCTGCGTGGTCGTACCTACGGTGCGATC
AACCGTCAGGACACCGCGCGTTTCGGTTCTCTGGCGTCTCGTCTGCTGCACCACATCAAC
TCTCTGAAAGAAGACCGTATCAAAACCGGTGCGGACTCTATCGTTCAGGCGGCGCGTGGT
TACATCCCGCTGCCGCACGGTAAAGGTTGGGAACAGCGTTACGAACCGTGCCAGCTGATC
CTGTTCGAAGACCTGGCGCGTTACCGTTTCCGTGTTGACCGTCCGCGTCGTGAAAACTCT
CAGCTGATGCAGTGGAACCACCGTGCGATCGTTGCGGAAACCACCATGCAGGCGGAACTG
TACGGTCAGATCGTTGAAAACACCGCGGCGGGTTTCTCTTCTCGTTTCCACGCGGCGACC
GGTGCGCCGGGTGTTCGTTGCCGTTTCCTGCTGGAACGTGACTTCGACAACGACCTGCCG
AAACCGTACCTGCTGCGTGAACTGTCTTGGATGCTGGGTAACACCAAAGTTGAATCTGAA
GAAGAAAAACTGCGTCTGCTGTCTGAAAAAATCCGTCCGGGTTCTCTGGTTCCGTGGGAC
GGTGGTGAACAGTTCGCGACCCTGCACCCGAAACGTCAGACCCTGTGCGTTATCCACGCG
GACATGAACGCGGCGCAGAACCTGCAGCGTCGTTTCTTCGGTCGTTGCGGTGAAGCGTTC
CGTCTGGTTTGCCAGCCGCACGGTGACGACGTTCTGCGTCTGGCGTCTACCCCGGGTGCG
CGTCTGCTGGGTGCGCTGCAGCAGCTGGAAAACGGTCAGGGTGCGTTCGAACTGGTTCGT
GACATGGGTTCTACCTCTCAGATGAACCGTTTCGTTATGAAATCTCTGGGTAAAAAAAAA
ATCAAACCGCTGCAGGACAACAACGGTGACGACGAACTGGAAGACGTTCTGTCTGTTCTG
CCGGAAGAAGACGACACCGGTCGTATCACCGTTTTCCGTGACTCTTCTGGTATCTTCTTC
CCGTGCAACGTTTGGATCCCGGCGAAACAGTTCTGGCCGGCGGTTCGTGCGATGATCTGG
AAAGTTATGGCGTCTCACTCTCTGGGTTAA
SEQ ATGACCAAACTGCGTCACCGTCAGAAAAAACTGACCCACGACTGGGCGGGTTCTAAAAAA
ID CGTGAAGTTCTGGGTTCTAACGGTAAACTGCAGAACCCGCTGCTGATGCCGGTTAAAAAA
NO: GGTCAGGTTACCGAATTCCGTAAAGCGTTCTCTGCGTACGCGCGTGCGACCAAAGGTGAA
56 ATGACCGACGGTCGTAAAAACATGTTCACCCACTCTTTCGAACCGTTCAAAACCAAACCG
TCTCTGCACCAGTGCGAACTGGCGGACAAAGCGTACCAGTCTCTGCACTCTTACCTGCCG
GGTTCTCTGGCGCACTTCCTGCTGTCTGCGCACGCGCTGGGTTTCCGTATCTTCTCTAAA
TCTGGTGAAGCGACCGCGTTCCAGGCGTCTTCTAAAATCGAAGCGTACGAATCTAAACTG
GCGTCTGAACTGGCGTGCGTTGACCTGTCTATCCAGAACCTGACCATCTCTACCCTGTTC
AACGCGCTGACCACCTCTGTTCGTGGTAAAGGTGAAGAAACCTCTGCGGACCCGCTGATC
GCGCGTTTCTACACCCTGCTGACCGGTAAACCGCTGTCTCGTGACACCCAGGGTCCGGAA
CGTGACCTGGCGGAAGTTATCTCTCGTAAAATCGCGTCTTCTTTCGGTACCTGGAAAGAA
ATGACCGCGAACCCGCTGCAGTCTCTGCAGTTCTTCGAAGAAGAACTGCACGCGCTGGAC
GCGAACGTTTCTCTGTCTCCGGCGTTCGACGTTCTGATCAAAATGAACGACCTGCAGGGT
GACCTGAAAAACCGTACCATCGTTTTCGACCCGGACGCGCCGGTTTTCGAATACAACGCG
GAAGACCCGGCGGACATCATCATCAAACTGACCGCGCGTTACGCGAAAGAAGCGGTTATC
AAAAACCAGAACGTTGGTAACTACGTTAAAAACGCGATCACCACCACCAACGCGAACGGT
CTGGGTTGGCTGCTGAACAAAGGTCTGTCTCTGCTGCCGGTTTCTACCGACGACGAACTG
CTGGAATTCATCGGTGTTGAACGTTCTCACCCGTCTTGCCACGCGCTGATCGAACTGATC
GCGCAGCTGGAAGCGCCGGAACTGTTCGAAAAAAACGTTTTCTCTGACACCCGTTCTGAA
GTTCAGGGTATGATCGACTCTGCGGTTTCTAACCACATCGCGCGTCTGTCTTCTTCTCGT
AACTCTCTGTCTATGGACTCTGAAGAACTGGAACGTCTGATCAAATCTTTCCAGATCCAC
ACCCCGCACTGCTCTCTGTTCATCGGTGCGCAGTCTCTGTCTCAGCAGCTGGAATCTCTG
CCGGAAGCGCTGCAGTCTGGTGTTAACTCTGCGGACATCCTGCTGGGTTCTACCCAGTAC
ATGCTGACCAACTCTCTGGTTGAAGAATCTATCGCGACCTACCAGCGTACCCTGAACCGT
ATCAACTACCTGTCTGGTGTTGCGGGTCAGATCAACGGTGCGATCAAACGTAAAGCGATC
GACGGTGAAAAAATCCACCTGCCGGCGGCGTGGTCTGAACTGATCTCTCTGCCGTTCATC
GGTCAGCCGGTTATCGACGTTGAATCTGACCTGGCGCACCTGAAAAACCAGTACCAGACC
CTGTCTAACGAATTCGACACCCTGATCTCTGCGCTGCAGAAAAACTTCGACCTGAACTTC
AACAAAGCGCTGCTGAACCGTACCCAGCACTTCGAAGCGATGTGCCGTTCTACCAAAAAA
AACGCGCTGTCTAAACCGGAAATCGTTTCTTACCGTGACCTGCTGGCGCGTCTGACCTCT
TGCCTGTACCGTGGTTCTCTGGTTCTGCGTCGTGCGGGTATCGAAGTTCTGAAAAAACAC
AAAATCTTCGAATCTAACTCTGAACTGCGTGAACACGTTCACGAACGTAAACACTTCGTT
TTCGTTTCTCCGCTGGACCGTAAAGCGAAAAAACTGCTGCGTCTGACCGACTCTCGTCCG
GACCTGCTGCACGTTATCGACGAAATCCTGCAGCACGACAACCTGGAAAACAAAGACCGT
GAATCTCTGTGGCTGGTTCGTTCTGGTTACCTGCTGGCGGGTCTGCCGGACCAGCTGTCT
TCTTCTTTCATCAACCTGCCGATCATCACCCAGAAAGGTGACCGTCGTCTGATCGACCTG
ATCCAGTACGACCAGATCAACCGTGACGCGTTCGTTATGCTGGTTACCTCTGCGTTCAAA
TCTAACCTGTCTGGTCTGCAGTACCGTGCGAACAAACAGTCTTTCGTTGTTACCCGTACC
CTGTCTCCGTACCTGGGTTCTAAACTGGTTTACGTTCCGAAAGACAAAGACTGGCTGGTT
CCGTCTCAGATGTTCGAAGGTCGTTTCGCGGACATCCTGCAGTCTGACTACATGGTTTGG
AAAGACGCGGGTCGTCTGTGCGTTATCGACACCGCGAAACACCTGTCTAACATCAAAAAA
TCTGTTTTCTCTTCTGAAGAAGTTCTGGCGTTCCTGCGTGAACTGCCGCACCGTACCTTC
ATCCAGACCGAAGTTCGTGGTCTGGGTGTTAACGTTGACGGTATCGCGTTCAACAACGGT
GACATCCCGTCTCTGAAAACCTTCTCTAACTGCGTTCAGGTTAAAGTTTCTCGTACCAAC
ACCTCTCTGGTTCAGACCCTGAACCGTTGGTTCGAAGGTGGTAAAGTTTCTCCGCCGTCT
ATCCAGTTCGAACGTGCGTACTACAAAAAAGACGACCAGATCCACGAAGACGCGGCGAAA
CGTAAAATCCGTTTCCAGATGCCGGCGACCGAACTGGTTCACGCGTCTGACGACGCGGGT
TGGACCCCGTCTTACCTGCTGGGTATCGACCCGGGTGAATACGGTATGGGTCTGTCTCTG
GTTTCTATCAACAACGGTGAAGTTCTGGACTCTGGTTTCATCCACATCAACTCTCTGATC
AACTTCGCGTCTAAAAAATCTAACCACCAGACCAAAGTTGTTCCGCGTCAGCAGTACAAA
TCTCCGTACGCGAACTACCTGGAACAGTCTAAAGACTCTGCGGCGGGTGACATCGCGCAC
ATCCTGGACCGTCTGATCTACAAACTGAACGCGCTGCCGGTTTTCGAAGCGCTGTCTGGT
AACTCTCAGTCTGCGGCGGACCAGGTTTGGACCAAAGTTCTGTCTTTCTACACCTGGGGT
GACAACGACGCGCAGAACTCTATCCGTAAACAGCACTGGTTCGGTGCGTCTCACTGGGAC
ATCAAAGGTATGCTGCGTCAGCCGCCGACCGAAAAAAAACCGAAACCGTACATCGCGTTC
CCGGGTTCTCAGGTTTCTTCTTACGGTAACTCTCAGCGTTGCTCTTGCTGCGGTCGTAAC
CCGATCGAACAGCTGCGTGAAATGGCGAAAGACACCTCTATCAAAGAACTGAAAATCCGT
AACTCTGAAATCCAGCTGTTCGACGGTACCATCAAACTGTTCAACCCGGACCCGTCTACC
GTTATCGAACGTCGTCGTCACAACCTGGGTCCGTCTCGTATCCCGGTTGCGGACCGTACC
TTCAAAAACATCTCTCCGTCTTCTCTGGAATTCAAAGAACTGATCACCATCGTTTCTCGT
TCTATCCGTCACTCTCCGGAATTCATCGCGAAAAAACGTGGTATCGGTTCTGAATACTTC
TGCGCGTACTCTGACTGCAACTCTTCTCTGAACTCTGAAGCGAACGCGGCGGCGAACGTT
GCGCAGAAATTCCAGAAACAGCTGTTCTTCGAACTGTAA
SEQ ATGAAACGTATCCTGAACTCTCTGAAAGTTGCGGCGCTGCGTCTGCTGTTCCGTGGTAAA
ID GGTTCTGAACTGGTTAAAACCGTTAAATACCCGCTGGTTTCTCCGGTTCAGGGTGCGGTT
NO: GAAGAACTGGCGGAAGCGATCCGTCACGACAACCTGCACCTGTTCGGTCAGAAAGAAATC
57 GTTGACCTGATGGAAAAAGACGAAGGTACCCAGGTTTACTCTGTTGTTGACTTCTGGCTG
GACACCCTGCGTCTGGGTATGTTCTTCTCTCCGTCTGCGAACGCGCTGAAAATCACCCTG
GGTAAATTCAACTCTGACCAGGTTTCTCCGTTCCGTAAAGTTCTGGAACAGTCTCCGTTC
TTCCTGGCGGGTCGTCTGAAAGTTGAACCGGCGGAACGTATCCTGTCTGTTGAAATCCGT
AAAATCGGTAAACGTGAAAACCGTGTTGAAAACTACGCGGCGGACGTTGAAACCTGCTTC
ATCGGTCAGCTGTCTTCTGACGAAAAACAGTCTATCCAGAAACTGGCGAACGACATCTGG
GACTCTAAAGACCACGAAGAACAGCGTATGCTGAAAGCGGACTTCTTCGCGATCCCGCTG
ATCAAAGACCCGAAAGCGGTTACCGAAGAAGACCCGGAAAACGAAACCGCGGGTAAACAG
AAACCGCTGGAACTGTGCGTTTGCCTGGTTCCGGAACTGTACACCCGTGGTTTCGGTTCT
ATCGCGGACTTCCTGGTTCAGCGTCTGACCCTGCTGCGTGACAAAATGTCTACCGACACC
GCGGAAGACTGCCTGGAATACGTTGGTATCGAAGAAGAAAAAGGTAACGGTATGAACTCT
CTGCTGGGTACCTTCCTGAAAAACCTGCAGGGTGACGGTTTCGAACAGATCTTCCAGTTC
ATGCTGGGTTCTTACGTTGGTTGGCAGGGTAAAGAAGACGTTCTGCGTGAACGTCTGGAC
CTGCTGGCGGAAAAAGTTAAACGTCTGCCGAAACCGAAATTCGCGGGTGAATGGTCTGGT
CACCGTATGTTCCTGCACGGTCAGCTGAAATCTTGGTCTTCTAACTTCTTCCGTCTGTTC
AACGAAACCCGTGAACTGCTGGAATCTATCAAATCTGACATCCAGCACGCGACCATGCTG
ATCTCTTACGTTGAAGAAAAAGGTGGTTACCACCCGCAGCTGCTGTCTCAGTACCGTAAA
CTGATGGAACAGCTGCCGGCGCTGCGTACCAAAGTTCTGGACCCGGAAATCGAAATGACC
CACATGTCTGAAGCGGTTCGTTCTTACATCATGATCCACAAATCTGTTGCGGGTTTCCTG
CCGGACCTGCTGGAATCTCTGGACCGTGACAAAGACCGTGAATTCCTGCTGTCTATCTTC
CCGCGTATCCCGAAAATCGACAAAAAAACCAAAGAAATCGTTGCGTGGGAACTGCCGGGT
GAACCGGAAGAAGGTTACCTGTTCACCGCGAACAACCTGTTCCGTAACTTCCTGGAAAAC
CCGAAACACGTTCCGCGTTTCATGGCGGAACGTATCCCGGAAGACTGGACCCGTCTGCGT
TCTGCGCCGGTTTGGTTCGACGGTATGGTTAAACAGTGGCAGAAAGTTGTTAACCAGCTG
GTTGAATCTCCGGGTGCGCTGTACCAGTTCAACGAATCTTTCCTGCGTCAGCGTCTGCAG
GCGATGCTGACCGTTTACAAACGTGACCTGCAGACCGAAAAATTCCTGAAACTGCTGGCG
GACGTTTGCCGTCCGCTGGTTGACTTCTTCGGTCTGGGTGGTAACGACATCATCTTCAAA
TCTTGCCAGGACCCGCGTAAACAGTGGCAGACCGTTATCCCGCTGTCTGTTCCGGCGGAC
GTTTACACCGCGTGCGAAGGTCTGGCGATCCGTCTGCGTGAAACCCTGGGTTTCGAATGG
AAAAACCTGAAAGGTCACGAACGTGAAGACTTCCTGCGTCTGCACCAGCTGCTGGGTAAC
CTGCTGTTCTGGATCCGTGACGCGAAACTGGTTGTTAAACTGGAAGACTGGATGAACAAC
CCGTGCGTTCAGGAATACGTTGAAGCGCGTAAAGCGATCGACCTGCCGCTGGAAATCTTC
GGTTTCGAAGTTCCGATCTTCCTGAACGGTTACCTGTTCTCTGAACTGCGTCAGCTGGAA
CTGCTGCTGCGTCGTAAATCTGTTATGACCTCTTACTCTGTTAAAACCACCGGTTCTCCG
AACCGTCTGTTCCAGCTGGTTTACCTGCCGCTGAACCCGTCTGACCCGGAAAAAAAAAAC
TCTAACAACTTCCAGGAACGTCTGGACACCCCGACCGGTCTGTCTCGTCGTTTCCTGGAC
CTGACCCTGGACGCGTTCGCGGGTAAACTGCTGACCGACCCGGTTACCCAGGAACTGAAA
ACCATGGCGGGTTTCTACGACCACCTGTTCGGTTTCAAACTGCCGTGCAAACTGGCGGCG
ATGTCTAACCACCCGGGTTCTTCTTCTAAAATGGTTGTTCTGGCGAAACCGAAAAAAGGT
GTTGCGTCTAACATCGGTTTCGAACCGATCCCGGACCCGGCGCACCCGGTTTTCCGTGTT
CGTTCTTCTTGGCCGGAACTGAAATACCTGGAAGGTCTGCTGTACCTGCCGGAAGACACC
CCGCTGACCATCGAACTGGCGGAAACCTCTGTTTCTTGCCAGTCTGTTTCTTCTGTTGCG
TTCGACCTGAAAAACCTGACCACCATCCTGGGTCGTGTTGGTGAATTCCGTGTTACCGCG
GACCAGCCGTTCAAACTGACCCCGATCATCCCGGAAAAAGAAGAATCTTTCATCGGTAAA
ACCTACCTGGGTCTGGACGCGGGTGAACGTTCTGGTGTTGGTTTCGCGATCGTTACCGTT
GACGGTGACGGTTACGAAGTTCAGCGTCTGGGTGTTCACGAAGACACCCAGCTGATGGCG
CTGCAGCAGGTTGCGTCTAAATCTCTGAAAGAACCGGTTTTCCAGCCGCTGCGTAAAGGT
ACCTTCCGTCAGCAGGAACGTATCCGTAAATCTCTGCGTGGTTGCTACTGGAACTTCTAC
CACGCGCTGATGATCAAATACCGTGCGAAAGTTGTTCACGAAGAATCTGTTGGTTCTTCT
GGTCTGGTTGGTCAGTGGCTGCGTGCGTTCCAGAAAGACCTGAAAAAAGCGGACGTTCTG
CCGAAAAAAGGTGGTAAAAACGGTGTTGACAAAAAAAAACGTGAATCTTCTGCGCAGGAC
ACCCTGTGGGGTGGTGCGTTCTCTAAAAAAGAAGAACAGCAGATCGCGTTCGAAGTTCAG
GCGGCGGGTTCTTCTCAGTTCTGCCTGAAATGCGGTTGGTGGTTCCAGCTGGGTATGCGT
GAAGTTAACCGTGTTCAGGAATCTGGTGTTGTTCTGGACTGGAACCGTTCTATCGTTACC
TTCCTGATCGAATCTTCTGGTGAAAAAGTTTACGGTTTCTCTCCGCAGCAGCTGGAAAAA
GGTTTCCGTCCGGACATCGAAACCTTCAAAAAAATGGTTCGTGACTTCATGCGTCCGCCG
ATGTTCGACCGTAAAGGTCGTCCGGCGGCGGCGTACGAACGTTTCGTTCTGGGTCGTCGT
CACCGTCGTTACCGTTTCGACAAAGTTTTCGAAGAACGTTTCGGTCGTTCTGCGCTGTTC
ATCTGCCCGCGTGTTGGTTGCGGTAACTTCGACCACTCTTCTGAACAGTCTGCGGTTGTT
CTGGCGCTGATCGGTTACATCGCGGACAAAGAAGGTATGTCTGGTAAAAAACTGGTTTAC
GTTCGTCTGGCGGAACTGATGGCGGAATGGAAACTGAAAAAACTGGAACGTTCTCGTGTT
GAAGAACAGTCTTCTGCGCAGTAA
SEQ ATGGCGGAATCTAAACAGATGCAGTGCCGTAAATGCGGTGCGTCTATGAAATACGAAGTT
ID ATCGGTCTGGGTAAAAAATCTTGCCGTTACATGTGCCCGGACTGCGGTAACCACACCTCT
NO: GCGCGTAAAATCCAGAACAAAAAAAAACGTGACAAAAAATACGGTTCTGCGTCTAAAGCG
58 CAGTCTCAGCGTATCGCGGTTGCGGGTGCGCTGTACCCGGACAAAAAAGTTCAGACCATC
AAAACCTACAAATACCCGGCGGACCTGAACGGTGAAGTTCACGACTCTGGTGTTGCGGAA
AAAATCGCGCAGGCGATCCAGGAAGACGAAATCGGTCTGCTGGGTCCGTCTTCTGAATAC
GCGTGCTGGATCGCGTCTCAGAAACAGTCTGAACCGTACTCTGTTGTTGACTTCTGGTTC
GACGCGGTTTGCGCGGGTGGTGTTTTCGCGTACTCTGGTGCGCGTCTGCTGTCTACCGTT
CTGCAGCTGTCTGGTGAAGAATCTGTTCTGCGTGCGGCGCTGGCGTCTTCTCCGTTCGTT
GACGACATCAACCTGGCGCAGGCGGAAAAATTCCTGGCGGTTTCTCGTCGTACCGGTCAG
GACAAACTGGGTAAACGTATCGGTGAATGCTTCGCGGAAGGTCGTCTGGAAGCGCTGGGT
ATCAAAGACCGTATGCGTGAATTCGTTCAGGCGATCGACGTTGCGCAGACCGCGGGTCAG
CGTTTCGCGGCGAAACTGAAAATCTTCGGTATCTCTCAGATGCCGGAAGCGAAACAGTGG
AACAACGACTCTGGTCTGACCGTTTGCATCCTGCCGGACTACTACGTTCCGGAAGAAAAC
CGTGCGGACCAGCTGGTTGTTCTGCTGCGTCGTCTGCGTGAAATCGCGTACTGCATGGGT
ATCGAAGACGAAGCGGGTTTCGAACACCTGGGTATCGACCCGGGTGCGCTGTCTAACTTC
TCTAACGGTAACCCGAAACGTGGTTTCCTGGGTCGTCTGCTGAACAACGACATCATCGCG
CTGGCGAACAACATGTCTGCGATGACCCCGTACTGGGAAGGTCGTAAAGGTGAACTGATC
GAACGTCTGGCGTGGCTGAAACACCGTGCGGAAGGTCTGTACCTGAAAGAACCGCACTTC
GGTAACTCTTGGGCGGACCACCGTTCTCGTATCTTCTCTCGTATCGCGGGTTGGCTGTCT
GGTTGCGCGGGTAAACTGAAAATCGCGAAAGACCAGATCTCTGGTGTTCGTACCGACCTG
TTCCTGCTGAAACGTCTGCTGGACGCGGTTCCGCAGTCTGCGCCGTCTCCGGACTTCATC
GCGTCTATCTCTGCGCTGGACCGTTTCCTGGAAGCGGCGGAATCTTCTCAGGACCCGGCG
GAACAGGTTCGTGCGCTGTACGCGTTCCACCTGAACGCGCCGGCGGTTCGTTCTATCGCG
AACAAAGCGGTTCAGCGTTCTGACTCTCAGGAATGGCTGATCAAAGAACTGGACGCGGTT
GACCACCTGGAATTCAACAAAGCGTTCCCGTTCTTCTCTGACACCGGTAAAAAAAAAAAA
AAAGGTGCGAACTCTAACGGTGCGCCGTCTGAAGAAGAATACACCGAAACCGAATCTATC
CAGCAGCCGGAAGACGCGGAACAGGAAGTTAACGGTCAGGAAGGTAACGGTGCGTCTAAA
AACCAGAAAAAATTCCAGCGTATCCCGCGTTTCTTCGGTGAAGGTTCTCGTTCTGAATAC
CGTATCCTGACCGAAGCGCCGCAGTACTTCGACATGTTCTGCAACAACATGCGTGCGATC
TTCATGCAGCTGGAATCTCAGCCGCGTAAAGCGCCGCGTGACTTCAAATGCTTCCTGCAG
AACCGTCTGCAGAAACTGTACAAACAGACCTTCCTGAACGCGCGTTCTAACAAATGCCGT
GCGCTGCTGGAATCTGTTCTGATCTCTTGGGGTGAATTCTACACCTACGGTGCGAACGAA
AAAAAATTCCGTCTGCGTCACGAAGCGTCTGAACGTTCTTCTGACCCGGACTACGTTGTT
CAGCAGGCGCTGGAAATCGCGCGTCGTCTGTTCCTGTTCGGTTTCGAATGGCGTGACTGC
TCTGCGGGTGAACGTGTTGACCTGGTTGAAATCCACAAAAAAGCGATCTCTTTCCTGCTG
GCGATCACCCAGGCGGAAGTTTCTGTTGGTTCTTACAACTGGCTGGGTAACTCTACCGTT
TCTCGTTACCTGTCTGTTGCGGGTACCGACACCCTGTACGGTACCCAGCTGGAAGAATTC
CTGAACGCGACCGTTCTGTCTCAGATGCGTGGTCTGGCGATCCGTCTGTCTTCTCAGGAA
CTGAAAGACGGTTTCGACGTTCAGCTGGAATCTTCTTGCCAGGACAACCTGCAGCACCTG
CTGGTTTACCGTGCGTCTCGTGACCTGGCGGCGTGCAAACGTGCGACCTGCCCGGCGGAA
CTGGACCCGAAAATCCTGGTTCTGCCGGTTGGTGCGTTCATCGCGTCTGTTATGAAAATG
ATCGAACGTGGTGACGAACCGCTGGCGGGTGCGTACCTGCGTCACCGTCCGCACTCTTTC
GGTTGGCAGATCCGTGTTCGTGGTGTTGCGGAAGTTGGTATGGACCAGGGTACCGCGCTG
GCGTTCCAGAAACCGACCGAATCTGAACCGTTCAAAATCAAACCGTTCTCTGCGCAGTAC
GGTCCGGTTCTGTGGCTGAACTCTTCTTCTTACTCTCAGTCTCAGTACCTGGACGGTTTC
CTGTCTCAGCCGAAAAACTGGTCTATGCGTGTTCTGCCGCAGGCGGGTTCTGTTCGTGTT
GAACAGCGTGTTGCGCTGATCTGGAACCTGCAGGCGGGTAAAATGCGTCTGGAACGTTCT
GGTGCGCGTGCGTTCTTCATGCCGGTTCCGTTCTCTTTCCGTCCGTCTGGTTCTGGTGAC
GAAGCGGTTCTGGCGCCGAACCGTTACCTGGGTCTGTTCCCGCACTCTGGTGGTATCGAA
TACGCGGTTGTTGACGTTCTGGACTCTGCGGGTTTCAAAATCCTGGAACGTGGTACCATC
GCGGTTAACGGTTTCTCTCAGAAACGTGGTGAACGTCAGGAAGAAGCGCACCGTGAAAAA
CAGCGTCGTGGTATCTCTGACATCGGTCGTAAAAAACCGGTTCAGGCGGAAGTTGACGCG
GCGAACGAACTGCACCGTAAATACACCGACGTTGCGACCCGTCTGGGTTGCCGTATCGTT
GTTCAGTGGGCGCCGCAGCCGAAACCGGGTACCGCGCCGACCGCGCAGACCGTTTACGCG
CGTGCGGTTCGTACCGAAGCGCCGCGTTCTGGTAACCAGGAAGACCACGCGCGTATGAAA
TCTTCTTGGGGTTACACCTGGGGTACCTACTGGGAAAAACGTAAACCGGAAGACATCCTG
GGTATCTCTACCCAGGTTTACTGGACCGGTGGTATCGGTGAATCTTGCCCGGCGGTTGCG
GTTGCGCTGCTGGGTCACATCCGTGCGACCTCTACCCAGACCGAATGGGAAAAAGAAGAA
GTTGTTTTCGGTCGTCTGAAAAAATTCTTCCCGTCTTAA
SEQ ATGGAAAAACGTATCAACAAAATCCGTAAAAAACTGTCTGCGGACAACGCGACCAAACCG
ID GTTTCTCGTTCTGGTCCGATGAAAACCCTGCTGGTTCGTGTTATGACCGACGACCTGAAA
NO: AAACGTCTGGAAAAACGTCGTAAAAAACCGGAAGTTATGCCGCAGGTTATCTCTAACAAC
59 GCGGCGAACAACCTGCGTATGCTGCTGGACGACTACACCAAAATGAAAGAAGCGATCCTG
CAGGTTTACTGGCAGGAATTCAAAGACGACCACGTTGGTCTGATGTGCAAATTCGCGCAG
CCGGCGTCTAAAAAAATCGACCAGAACAAACTGAAACCGGAAATGGACGAAAAAGGTAAC
CTGACCACCGCGGGTTTCGCGTGCTCTCAGTGCGGTCAGCCGCTGTTCGTTTACAAACTG
GAACAGGTTTCTGAAAAAGGTAAAGCGTACACCAACTACTTCGGTCGTTGCAACGTTGCG
GAACACGAAAAACTGATCCTGCTGGCGCAGCTGAAACCGGAAAAAGACTCTGACGAAGCG
GTTACCTACTCTCTGGGTAAATTCGGTCAGCGTGCGCTGGACTTCTACTCTATCCACGTT
ACCAAAGAATCTACCCACCCGGTTAAACCGCTGGCGCAGATCGCGGGTAACCGTTACGCG
TCTGGTCCGGTTGGTAAAGCGCTGTCTGACGCGTGCATGGGTACCATCGCGTCTTTCCTG
TCTAAATACCAGGACATCATCATCGAACACCAGAAAGTTGTTAAAGGTAACCAGAAACGT
CTGGAATCTCTGCGTGAACTGGCGGGTAAAGAAAACCTGGAATACCCGTCTGTTACCCTG
CCGCCGCAGCCGCACACCAAAGAAGGTGTTGACGCGTACAACGAAGTTATCGCGCGTGTT
CGTATGTGGGTTAACCTGAACCTGTGGCAGAAACTGAAACTGTCTCGTGACGACGCGAAA
CCGCTGCTGCGTCTGAAAGGTTTCCCGTCTTTCCCGGTTGTTGAACGTCGTGAAAACGAA
GTTGACTGGTGGAACACCATCAACGAAGTTAAAAAACTGATCGACGCGAAACGTGACATG
GGTCGTGTTTTCTGGTCTGGTGTTACCGCGGAAAAACGTAACACCATCCTGGAAGGTTAC
AACTACCTGCCGAACGAAAACGACCACAAAAAACGTGAAGGTTCTCTGGAAAACCCGAAA
AAACCGGCGAAACGTCAGTTCGGTGACCTGCTGCTGTACCTGGAAAAAAAATACGCGGGT
GACTGGGGTAAAGTTTTCGACGAAGCGTGGGAACGTATCGACAAAAAAATCGCGGGTCTG
ACCTCTCACATCGAACGTGAAGAAGCGCGTAACGCGGAAGACGCGCAGTCTAAAGCGGTT
CTGACCGACTGGCTGCGTGCGAAAGCGTCTTTCGTTCTGGAACGTCTGAAAGAAATGGAC
GAAAAAGAATTCTACGCGTGCGAAATCCAGCTGCAGAAATGGTACGGTGACCTGCGTGGT
AACCCGTTCGCGGTTGAAGCGGAAAACCGTGTTGTTGACATCTCTGGTTTCTCTATCGGT
TCTGACGGTCACTCTATCCAGTACCGTAACCTGCTGGCGTGGAAATACCTGGAAAACGGT
AAACGTGAATTCTACCTGCTGATGAACTACGGTAAAAAAGGTCGTATCCGTTTCACCGAC
GGTACCGACATCAAAAAATCTGGTAAATGGCAGGGTCTGCTGTACGGTGGTGGTAAAGCG
AAAGTTATCGACCTGACCTTCGACCCGGACGACGAACAGCTGATCATCCTGCCGCTGGCG
TTCGGTACCCGTCAGGGTCGTGAATTCATCTGGAACGACCTGCTGTCTCTGGAAACCGGT
CTGATCAAACTGGCGAACGGTCGTGTTATCGAAAAAACCATCTACAACAAAAAAATCGGT
CGTGACGAACCGGCGCTGTTCGTTGCGCTGACCTTCGAACGTCGTGAAGTTGTTGACCCG
TCTAACATCAAACCGGTTAACCTGATCGGTGTTGACCGTGGTGAAAACATCCCGGCGGTT
ATCGCGCTGACCGACCCGGAAGGTTGCCCGCTGCCGGAATTCAAAGACTCTTCTGGTGGT
CCGACCGACATCCTGCGTATCGGTGAAGGTTACAAAGAAAAACAGCGTGCGATCCAGGCG
GCGAAAGAAGTTGAACAGCGTCGTGCGGGTGGTTACTCTCGTAAATTCGCGTCTAAATCT
CGTAACCTGGCGGACGACATGGTTCGTAACTCTGCGCGTGACCTGTTCTACCACGCGGTT
ACCCACGACGCGGTTCTGGTTTTCGAAAACCTGTCTCGTGGTTTCGGTCGTCAGGGTAAA
CGTACCTTCATGACCGAACGTCAGTACACCAAAATGGAAGACTGGCTGACCGCGAAACTG
GCGTACGAAGGTCTGACCTCTAAAACCTACCTGTCTAAAACCCTGGCGCAGTACACCTCT
AAAACCTGCTCTAACTGCGGTTTCACCATCACCACCGCGGACTACGACGGTATGCTGGTT
CGTCTGAAAAAAACCTCTGACGGTTGGGCGACCACCCTGAACAACAAAGAACTGAAAGCG
GAAGGTCAGATCACCTACTACAACCGTTACAAACGTCAGACCGTTGAAAAAGAACTGTCT
GCGGAACTGGACCGTCTGTCTGAAGAATCTGGTAACAACGACATCTCTAAATGGACCAAA
GGTCGTCGTGACGAAGCGCTGTTCCTGCTGAAAAAACGTTTCTCTCACCGTCCGGTTCAG
GAACAGTTCGTTTGCCTGGACTGCGGTCACGAAGTTCACGCGGACGAACAGGCGGCGCTG
AACATCGCGCGTTCTTGGCTGTTCCTGAACTCTAACTCTACCGAATTCAAATCTTACAAA
TCTGGTAAACAGCCGTTCGTTGGTGCGTGGCAGGCGTTCTACAAACGTCGTCTGAAAGAA
GTTTGGAAACCGAACGCG
SEQ ATGAAACGTATCAACAAAATCCGTCGTCGTCTGGTTAAAGACTCTAACACCAAAAAAGCG
ID GGTAAAACCGGTCCGATGAAAACCCTGCTGGTTCGTGTTATGACCCCGGACCTGCGTGAA
NO: CGTCTGGAAAACCTGCGTAAAAAACCGGAAAACATCCCGCAGCCGATCTCTAACACCTCT
60 CGTGCGAACCTGAACAAACTGCTGACCGACTACACCGAAATGAAAAAAGCGATCCTGCAC
GTTTACTGGGAAGAATTCCAGAAAGACCCGGTTGGTCTGATGTCTCGTGTTGCGCAGCCG
GCGCCGAAAAACATCGACCAGCGTAAACTGATCCCGGTTAAAGACGGTAACGAACGTCTG
ACCTCTTCTGGTTTCGCGTGCTCTCAGTGCTGCCAGCCGCTGTACGTTTACAAACTGGAA
CAGGTTAACGACAAAGGTAAACCGCACACCAACTACTTCGGTCGTTGCAACGTTTCTGAA
CACGAACGTCTGATCCTGCTGTCTCCGCACAAACCGGAAGCGAACGACGAACTGGTTACC
TACTCTCTGGGTAAATTCGGTCAGCGTGCGCTGGACTTCTACTCTATCCACGTTACCCGT
GAATCTAACCACCCGGTTAAACCGCTGGAACAGATCGGTGGTAACTCTTGCGCGTCTGGT
CCGGTTGGTAAAGCGCTGTCTGACGCGTGCATGGGTGCGGTTGCGTCTTTCCTGACCAAA
TACCAGGACATCATCCTGGAACACCAGAAAGTTATCAAAAAAAACGAAAAACGTCTGGCG
AACCTGAAAGACATCGCGTCTGCGAACGGTCTGGCGTTCCCGAAAATCACCCTGCCGCCG
CAGCCGCACACCAAAGAAGGTATCGAAGCGTACAACAACGTTGTTGCGCAGATCGTTATC
TGGGTTAACCTGAACCTGTGGCAGAAACTGAAAATCGGTCGTGACGAAGCGAAACCGCTG
CAGCGTCTGAAAGGTTTCCCGTCTTTCCCGCTGGTTGAACGTCAGGCGAACGAAGTTGAC
TGGTGGGACATGGTTTGCAACGTTAAAAAACTGATCAACGAAAAAAAAGAAGACGGTAAA
GTTTTCTGGCAGAACCTGGCGGGTTACAAACGTCAGGAAGCGCTGCTGCCGTACCTGTCT
TCTGAAGAAGACCGTAAAAAAGGTAAAAAATTCGCGCGTTACCAGTTCGGTGACCTGCTG
CTGCACCTGGAAAAAAAACACGGTGAAGACTGGGGTAAAGTTTACGACGAAGCGTGGGAA
CGTATCGACAAAAAAGTTGAAGGTCTGTCTAAACACATCAAACTGGAAGAAGAACGTCGT
TCTGAAGACGCGCAGTCTAAAGCGGCGCTGACCGACTGGCTGCGTGCGAAAGCGTCTTTC
GTTATCGAAGGTCTGAAAGAAGCGGACAAAGACGAATTCTGCCGTTGCGAACTGAAACTG
CAGAAATGGTACGGTGACCTGCGTGGTAAACCGTTCGCGATCGAAGCGGAAAACTCTATC
CTGGACATCTCTGGTTTCTCTAAACAGTACAACTGCGCGTTCATCTGGCAGAAAGACGGT
GTTAAAAAACTGAACCTGTACCTGATCATCAACTACTTCAAAGGTGGTAAACTGCGTTTC
AAAAAAATCAAACCGGAAGCGTTCGAAGCGAACCGTTTCTACACCGTTATCAACAAAAAA
TCTGGTGAAATCGTTCCGATGGAAGTTAACTTCAACTTCGACGACCCGAACCTGATCATC
CTGCCGCTGGCGTTCGGTAAACGTCAGGGTCGTGAATTCATCTGGAACGACCTGCTGTCT
CTGGAAACCGGTTCTCTGAAACTGGCGAACGGTCGTGTTATCGAAAAAACCCTGTACAAC
CGTCGTACCCGTCAGGACGAACCGGCGCTGTTCGTTGCGCTGACCTTCGAACGTCGTGAA
GTTCTGGACTCTTCTAACATCAAACCGATGAACCTGATCGGTATCGACCGTGGTGAAAAC
ATCCCGGCGGTTATCGCGCTGACCGACCCGGAAGGTTGCCCGCTGTCTCGTTTCAAAGAC
TCTCTGGGTAACCCGACCCACATCCTGCGTATCGGTGAATCTTACAAAGAAAAACAGCGT
ACCATCCAGGCGGCGAAAGAAGTTGAACAGCGTCGTGCGGGTGGTTACTCTCGTAAATAC
GCGTCTAAAGCGAAAAACCTGGCGGACGACATGGTTCGTAACACCGCGCGTGACCTGCTG
TACTACGCGGTTACCCAGGACGCGATGCTGATCTTCGAAAACCTGTCTCGTGGTTTCGGT
CGTCAGGGTAAACGTACCTTCATGGCGGAACGTCAGTACACCCGTATGGAAGACTGGCTG
ACCGCGAAACTGGCGTACGAAGGTCTGCCGTCTAAAACCTACCTGTCTAAAACCCTGGCG
CAGTACACCTCTAAAACCTGCTCTAACTGCGGTTTCACCATCACCTCTGCGGACTACGAC
CGTGTTCTGGAAAAACTGAAAAAAACCGCGACCGGTTGGATGACCACCATCAACGGTAAA
GAACTGAAAGTTGAAGGTCAGATCACCTACTACAACCGTTACAAACGTCAGAACGTTGTT
AAAGACCTGTCTGTTGAACTGGACCGTCTGTCTGAAGAATCTGTTAACAACGACATCTCT
TCTTGGACCAAAGGTCGTTCTGGTGAAGCGCTGTCTCTGCTGAAAAAACGTTTCTCTCAC
CGTCCGGTTCAGGAAAAATTCGTTTGCCTGAACTGCGGTTTCGAAACCCACGCGGACGAA
CAGGCGGCGCTGAACATCGCGCGTTCTTGGCTGTTCCTGCGTTCTCAGGAATACAAAAAA
TACCAGACCAACAAAACCACCGGTAACACCGACAAACGTGCGTTCGTTGAAACCTGGCAG
TCTTTCTACCGTAAAAAACTGAAAGAAGTTTGGAAACCG
SEQ AAAATTCcatGCAAAATGCTCCGGTTTCATGTCATCAAAATGATGACGTAATTAAGCATT
ID GATAATTGAGATCCCTCTCCCTGACAGGATGATTACATAAATAATAGTGACAAAAATAAA
NO: TTATTTATTTATCCAGAAAATGAATTGGAAAATCAGGAGAGCGTTTTCAATCCTACCTCT
61 GGCGCAGTTGATATGTcaaaCAGGTtgccgtcactgcgtcttttactggctcttctcgct
aaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccat
gacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattattt
gcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctac
ctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagcac
cgcctatctcgtgtgagataggcggagatacgaactttaagAAGGAGatataccATGGGT
AAAATGTATTACCTTGGTTTAGACATTGGCACGAATTCCGTGGGCTACGCGGTGACCGAC
CCCTCATACCACCTGCTGAAGTTTAAGGGGGAACCAATGTGGGGTGCGCACGTATTTGCC
GCCGGTAATCAGAGCGCGGAACGACGCTCGTTCCGCACATCGCGTCGTCGTTTGGACCGA
CGCCAACAGCGCGTTAAACTGGTACAGGAGATTTTTGCCCCGGTGATTAGTCCGATCGAC
CCACGCTTCTTCATTCGTCTGCATGAATCCGCCCTGTGGCGCGATGACGTCGCGGAGACG
GATAAACATATCTTTTTCAATGATCCTACCTATACCGATAAGGAATATTATAGCGATTAC
CCGACTATCCATCACCTGATCGTTGATCTGATGGAAAGCTCTGAGAAACACGATCCGCGG
CTGGTGTACCTTGCAGTGGCGTGGTTAGTGGCACACCGTGGTCATTTTCTGAACGAGGTG
GACAAGGATAATATTGGAGATGTGTTGTCGTTCGACGCATTTTATCCGGAGTTTCTCGCG
TTCCTGTCGGACAACGGTGTATCACCGTGGGTGTGCGAAAGCAAAGCGCTGCAGGCGACC
TTGCTGAGCCGTAACTCAGTGAACGACAAATATAAAGCCCTTAAGTCTCTGATCTTCGGA
TCCCAGAAACCTGAAGATAACTTCGATGCCAATATTTCGGAAGATGGACTCATTCAACTG
CTGGCCGGCAAAAAGGTAAAAGTTAACAAACTGTTCCCTCAGGAATCGAACGATGCATCC
TTCACATTGAATGATAAAGAAGACGCGATAGAAGAAATCCTGGGTACGCTTACACCAGAT
GAATGTGAATGGATTGCGCATATACGCCGCCTTTTTGACTGGGCTATCATGAAACATGCT
CTGAAAGATGGCAGGACTATTAGCGAGTCAAAAGTCAAACTGTATGAGCAGCACCATCAC
GATCTGACCCAACTTAAATACTTCGTGAAAACCTACCTTGCAAAAGAATACGACGATATT
TTCCGCAACGTGGATAGCGAAACAACGAAAAACTATGTAGCGTATTCCTATCATGTGAAA
GAGGTGAAAGGCACTCTGCCTAAAAATAAGGCAACGCAAGAAGAGTTTTGTAAGTATGTC
CTGGGCAAGGTTAAAAACATTGAATGCTCTGAAGCAGACAAGGTTGACTTTGATGAGATG
ATTCAGCGTCTTACCGACAACTCTTTTATGCCTAAGCAGGTTTCGGGCGAAAACCGCGTT
ATTCCTTATCAGTTATATTATTATGAACTGAAGACAATTCTGAATAAAGCAGCCTCGTAC
CTGCCTTTCCTGACGCAGTGTGGAAAAGATGCAATTTCGAACCAGGACAAACTACTGTCG
ATCATGACGTTCCGTATTCCTTACTTCGTCGGACCCTTGCGAAAAGATAATTCGGAACAT
GCATGGCTCGAACGAAAGGCCGGTAAGATTTATCCGTGGAACTTTAACGACAAAGTGGAC
TTGGATAAATCAGAAGAAGCGTTCATTCGCCGAATGACCAATACCTGTACCTATTATCCC
GGCGAAGATGTTTTACCGTTGGATTCGCTGATCTATGAGAAATTTATGATTTTAAATGAA
ATCAATAATATTCGTATTGACGGCTACCCGATTAGTGTTGACGTTAAACAGCAGGTTTTT
GGCTTGTTCGAAAAAAAACGACGCGTAACCGTGAAAGATATTCAGAACCTGCTGCTGTCT
CTCGGAGCTCTGGACAAACACGGGAAGCTGACAGGCATCGATACCACTATCCACTCAAAC
TATAATACGTATCACCATTTTAAATCTCTCATGGAACGCGGCGTCCTGACCCGGGATGAC
GTGGAACGCATCGTTGAAAGGATGACCTACAGCGACGATACTAAGCGTGTGCGTCTGTGG
CTGAATAACAACTATGGTACTTTAACCGCCGACGATGTGAAACACATTTCGCGTCTGCGC
AAACACGATTTTGGCCGTTTATCCAAAATGTTCTTAACAGGTCTGAAGGGTGTCCATAAG
GAGACCGGTGAACGTGCCTCCATACTGGATTTCATGTGGAACACGAACGATAACCTGATG
CAGCTCCTTTCCGAATGCTACACGTTCAGTGATGAAATCACAAAGCTGCAAGAGGCGTAT
TATGCAAAAGCCCAGTTGTCTTTAAACGATTTTTTAGACTCGATGTACATCTCTAACGCG
GTGAAACGTCCGATTTACAGAACTCTGGCAGTGGTGAACGATATTCGAAAAGCATGTGGG
ACGGCCCCTAAACGCATTTTCATCGAAATGGCTCGTGATGGTGAATCAAAAAAAAAGAGA
AGTGTTACACGTCGCGAGCAGATCAAAAACCTGTACCGCTCGATTCGTAAAGATTTCCAG
CAGGAAGTTGATTTTCTGGAAAAGATCCTGGAAAATAAATCTGATGGTCAACTTCAGTCA
GATGCTTTGTATCTTTACTTTGCACAATTAGGGCGCGATATGTACACGGGCGATCCAATA
AAGCTGGAGCACATCAAAGATCAGAGTTTCTATAACATAGACCATATTTACCCGCAGTCT
ATGGTGAAAGACGATTCCCTAGATAACAAAGTGCTGGTGCAAAGCGAAATTAACGGCGAG
AAAAGCTCGCGATACCCTTTGGACGCCGCGATCCGCAATAAAATGAAGCCCCTTTGGGAC
GCTTACTATAATCATGGCCTGATCTCCTTAAAGAAATACCAGCGTCTAACGCGCTCGACC
CCGTTTACCGATGATGAAAAATGGGACTTTATTAATCGCCAGTTAGTGGAAACCCGTCAA
TCTACCAAAGCGCTGGCCATTTTGTTGAAGCGTAAGTTTCCAGACACCGAAATTGTGTAT
TCGAAGGCGGGGTTATCGTCCGACTTCAGACATGAATTCGGCCTTGTAAAAAGTCGCAAT
ATTAATGATTTGCACCACGCTAAAGACGCATTCTTGGCTATCGTTACCGGCAATGTGTAC
CATGAAAGATTCAATCGCAGATGGTTTATGGTGAACCAGCCGTACTCAGTTAAAACTAAA
ACTCTTTTTACCCACAGCATAAAGAATGGCAACTTCGTTGCCTGGAACGGCGAAGAAGAT
CTCGGTCGTATTGTAAAAATGCTGAAGCAAAACAAAAATACCATTCACTTCACGCGCTTC
TCCTTCGATCGCAAAGAAGGATTATTTGATATCCAACCTCTGAAAGCCAGCACCGGCTTA
GTCCCACGAAAAGCCGGTCTGGATGTCGTTAAATACGGCGGATATGACAAATCTACCGCG
GCCTATTACCTGCTGGTGAGGTTCACGCTCGAGGACAAGAAAACCCAGCACAAGCTGATG
ATGATTCCTGTAGAAGGCCTGTACAAGGCTCGCATTGATCATGACAAGGAATTTCTTACC
GATTATGCGCAAACGACTATAAGCGAAATCCTACAGAAAGATAAACAGAAAGTGATCAAT
ATTATGTTTCCAATGGGTACGAGGCATATAAAACTCAATTCAATGATTAGTATCGATGGC
TTCTATCTTAGTATCGGCGGAAAGTCCTCTAAAGGTAAGTCAGTTCTATGTCACGCAATG
GTTCCACTGATCGTCCCTCACAAAATCGAATGTTACATTAAAGCAATGGAAAGCTTCGCC
CGGAAGTTTAAAGAAAACAACAAGCTGCGCATCGTAGAAAAATTCGATAAAATCACCGTT
GAAGACAACCTGAATCTCTACGAGCTCTTTCTCCAAAAACTGCAGCATAATCCCTATAAT
AAGTTTTTTTCGACACAGTTTGACGTACTGACGAACGGCCGTTCTACTTTCACAAAACTG
TCGCCGGAGGAACAGGTACAGACGCTCTTGAACATTTTAAGTATCTTTAAAACATGCCGC
AGTTCGGGTTGCGACCTGAAATCCATCAACGGCAGTGCCCAGGCAGCGCGCATCATGATT
AGCGCTGACTTAACTGGACTGTCGAAAAAATATTCAGATATTAGGTTGGTTGAACAGTCA
GCTTCTGGTTTGTTCGTATCCAAAAGTCAGAACTTACTGGAGTATCTCTAAGAAATCATC
CTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATTTATTATATCGCGTTGATTATTGAT
GCTGTTTTTAGTTTTAACGGCAATTAATATATGTGTTATTAATTGAATGAATTTTATCAT
TCATAATAAGTATGTGTAGGATCAAGCTCAGGTTAAATATTCACTCAGGAAGTTATTACT
CAGGAAGCAAAGAGGATTACAGAATTATCTCATAACAAGTGTTAAGGGATGTTATTTCC
SEQ AAAATTCcatGCAAAATGCTCCGGTTTCATGTCATCAAAATGATGACGTAATTAAGCATT
ID GATAATTGAGATCCCTCTCCCTGACAGGATGATTACATAAATAATAGTGACAAAAATAAA
NO: TTATTTATTTATCCAGAAAATGAATTGGAAAATCAGGAGAGCGTTTTCAATCCTACCTCT
62 GGCGCAGTTGATATGTcaaaCAGGTtgccgtcactgcgtcttttactggctcttctcgct
aaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccat
gacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattattt
gcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctac
ctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagcac
cgcctatctcgtgtgagataggcggagatacgaactttaagAAGGAGatataccATGTCA
TCGCTCACGAAATTCACTAACAAATACTCTAAACAGCTCACCATTAAGAATGAACTCATC
CCAGTTGGCAAAACACTGGAGAACATCAAAGAGAATGGTCTGATAGATGGCGACGAACAG
CTGAATGAGAATTATCAGAAGGCGAAAATTATTGTGGATGATTTTCTGCGGGACTTCATT
AATAAAGCACTGAATAATACGCAGATCGGGAACTGGCGCGAACTGGCGGATGCCCTTAAT
AAAGAGGATGAAGATAACATCGAGAAATTGCAGGATAAAATTCGGGGAATCATTGTATCC
AAATTTGAAACGTTTGATCTGTTTAGCAGCTATTCTATTAAGAAAGATGAAAAGATTATT
GACGACGACAATGATGTTGAAGAAGAGGAACTGGATCTGGGCAAGAAGACCAGCTCATTT
AAATACATATTTAAAAAAAACCTGTTTAAGTTAGTGTTGCCATCCTACCTGAAAACCACA
AACCAGGACAAGCTGAAGATTATTAGCTCGTTTGATAATTTTTCAACGTACTTCCGCGGG
TTCTTTGAAAACCGGAAAAACATTTTTACCAAGAAACCGATCTCCACAAGTATTGCGTAT
CGCATTGTTCATGATAACTTCCCGAAATTCCTTGATAACATTCGTTGTTTTAATGTGTGG
CAGACGGAATGCCCGCAACTAATCGTGAAAGCAGATAACTATCTGAAAAGCAAAAATGTT
ATAGCGAAAGATAAAAGTTTGGCAAACTATTTTACCGTGGGCGCGTATGACTATTTCCTG
TCTCAGAATGGTATAGATTTTTACAACAATATTATAGGTGGACTGCCAGCGTTCGCCGGC
CATGAGAAAATCCAAGGTCTCAATGAATTCATCAATCAAGAGTGCCAAAAAGACAGCGAG
CTGAAAAGTAAGCTGAAAAACCGTCACGCGTTCAAAATGGCGGTACTGTTCAAACAGATA
CTCAGCGATCGTGAAAAAAGTTTTGTAATTGATGAGTTCGAGTCGGATGCTCAAGTTATT
GACGCCGTTAAAAACTTTTACGCCGAACAGTGCAAAGATAACAATGTTATTTTTAACTTA
TTAAATCTTATCAAGAATATCGCTTTCTTAAGTGATGACGAACTGGACGGCATATTCATT
GAAGGGAAATACCTGTCGAGCGTTAGTCAAAAACTCTATAGCGATTGGTCAAAATTACGT
AACGACATTGAGGATTCGGCTAACTCTAAACAAGGCAATAAAGAGCTGGCCAAGAAGATC
AAAACCAACAAAGGGGATGTAGAAAAAGCGATCTCGAAATATGAGTTCTCGCTGTCGGAA
CTGAACTCGATTGTACATGATAACACCAAGTTTTCTGACCTCCTTAGTTGTACACTGCAT
AAGGTGGCTTCTGAGAAACTGGTGAAGGTCAATGAAGGCGACTGGCCGAAACATCTCAAG
AATAATGAAGAGAAACAAAAAATCAAAGAGCCGCTTGATGCTCTGCTGGAGATCTATAAT
ACACTTCTGATTTTTAACTGCAAAAGCTTCAATAAAAACGGCAACTTCTATGTCGACTAT
GATCGTTGCATCAATGAACTGAGTTCGGTCGTGTATCTGTATAATAAAACACGTAACTAT
TGCACTAAAAAACCCTATAACACGGACAAGTTCAAACTCAATTTTAACAGTCCGCAGCTC
GGTGAAGGCTTTTCCAAGTCGAAAGAAAATGACTGTCTGACTCTTTTGTTTAAAAAAGAC
GACAACTATTATGTAGGCATTATCCGCAAAGGTGCAAAAATCAATTTTGATGATACACAA
GCAATCGCCGATAACACCGACAATTGCATCTTTAAAATGAATTATTTCCTACTTAAAGAC
GCAAAAAAATTTATCCCGAAATGTAGCATTCAGCTGAAAGAAGTCAAGGCCCATTTTAAG
AAATCTGAAGATGATTACATTTTGTCTGATAAAGAGAAATTTGCTAGCCCGCTGGTCATT
AAAAAGAGCACATTTTTGCTGGCAACTGCACATGTGAAAGGGAAAAAAGGCAATATCAAG
AAATTTCAGAAAGAATATTCGAAAGAAAACCCCACTGAGTATCGCAATTCTTTAAACGAA
TGGATTGCTTTTTGTAAAGAGTTCTTAAAAACTTATAAAGCGGCTACCATTTTTGATATA
ACCACATTGAAAAAGGCAGAGGAATATGCTGATATTGTAGAATTCTACAAGGATGTCGAT
AATCTGTGCTACAAACTGGAGTTCTGCCCGATTAAAACCTCGTTTATAGAAAACCTGATA
GATAACGGCGACCTGTATCTGTTTCGCATCAATAACAAAGACTTCAGCAGTAAATCGACC
GGCACCAAGAACCTTCATACGTTATATTTACAAGCTATATTCGATGAACGTAATCTGAAC
AATCCGACAATTATGCTGAATGGGGGAGCAGAACTGTTCTATCGTAAAGAAAGTATTGAG
CAGAAAAACCGTATCACACACAAAGCCGGTTCAATTCTCGTGAATAAGGTGTGTAAAGAC
GGTACAAGCCTGGATGATAAGATACGTAATGAAATTTATCAATATGAGAATAAATTTATT
GATACCCTGTCTGATGAAGCTAAAAAGGTGTTACCGAATGTCATTAAAAAGGAAGCTACC
CATGACATTACAAAAGATAAACGTTTCACTAGTGACAAATTCTTCTTTCACTGCCCCCTG
ACAATTAATTATAAGGAAGGCGATACCAAGCAGTTCAATAACGAAGTGCTGAGTTTTCTG
CGTGGAAATCCTGACATCAACATTATCGGCATTGACCGCGGAGAGCGTAATTTAATCTAT
GTAACGGTTATAAACCAGAAAGGCGAGATTCTGGATTCGGTTTCATTCAATACCGTGACC
AACAAGAGTTCAAAAATCGAGCAGACAGTCGATTATGAAGAGAAATTGGCAGTCCGCGAG
AAAGAGAGGATTGAAGCAAAACGTTCCTGGGACTCTATCTCAAAAATTGCGACACTAAAG
GAAGGTTATCTGAGCGCAATAGTTCACGAGATCTGTCTGTTAATGATTAAACACAACGCG
ATCGTTGTCTTAGAGAATCTTAATGCAGGCTTTAAGCGTATTCGTGGCGGTTTATCAGAA
AAAAGTGTTTATCAAAAATTCGAAAAAATGTTGATTAACAAACTGAACTATTTTGTCAGC
AAGAAGGAATCCGACTGGAATAAACCGTCTGGTCTGCTGAATGGACTGCAGCTTTCGGAT
CAGTTTGAAAGCTTCGAAAAACTGGGTATTCAGTCTGGTTTTATTTTTTACGTGCCGGCT
GCATATACCTCAAAGATTGATCCGACCACGGGCTTCGCCAATGTTCTGAATCTGTCGAAG
GTACGCAATGTTGATGCGATCAAAAGCTTTTTTTCTAACTTCAACGAAATTAGTTATAGC
AAGAAAGAAGCCCTTTTCAAATTCTCATTCGATCTGGATTCACTGAGTAAGAAAGGCTTT
AGTAGCTTTGTGAAATTTAGTAAGAGTAAATGGAACGTCTACACCTTTGGAGAACGTATC
ATAAAGCCAAAGAATAAGCAAGGTTATCGGGAGGACAAAAGAATCAACTTGACCTTCGAG
ATGAAGAAGTTACTTAACGAGTATAAGGTTTCTTTTGATCTTGAAAATAACTTGATTCCG
AATCTCACGAGTGCCAACCTGAAGGATACTTTTTGGAAAGAGCTATTCTTTATCTTCAAG
ACTACGCTGCAGCTCCGTAACAGCGTTACTAACGGTAAAGAAGATGTGCTCATCTCTCCG
GTCAAAAATGCGAAGGGTGAATTCTTCGTTTCGGGAACGCATAACAAGACTCTTCCGCAA
GATTGCGATGCGAACGGTGCATACCATATTGCGTTGAAAGGTCTGATGATACTCGAACGT
AACAACCTTGTACGTGAGGAGAAAGATACGAAAAAGATTATGGCGATTTCAAACGTGGAT
TGGTTCGAGTACGTGCAGAAACGTAGAGGCGTTCTGTAAGAAATCATCCTTAGCGAAAGC
TAAGGATTTTTTTTATCTGAAATTTATTATATCGCGTTGATTATTGATGCTGTTTTTAGT
TTTAACGGCAATTAATATATGTGTTATTAATTGAATGAATTTTATCATTCATAATAAGTA
TGTGTAGGATCAAGCTCAGGTTAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAG
AGGATTACAGAATTATCTCATAACAAGTGTTAAGGGATGTTATTTCC
SEQ AATCAGGAGAGCGTTTTCAATCCTACCTCTGGCGCAGTTGATATGTCAAACAGGTTGCCG
ID TCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAG
NO: CATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAA
63 TCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC
ATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGT
TTCTCCATACCCGTTTTTTTGGGCTAGCAGTAATACGACTCACTATAGGGGTCTCATCTC
GTGTGAGATAGGCGGAGATACGAACTTTAAGAGGAGGATATACCATGCACCATCATCATC
ACCATAACAACTACGACGAATTCACCAAACTGTACCCGATCCAGAAAACCATCCGTTTCG
AACTGAAACCGCAGGGTCGTACCATGGAACACCTGGAAACCTTCAACTTCTTCGAAGAAG
ACCGTGACCGTGCGGAAAAATACAAAATCCTGAAAGAAGCGATCGACGAATACCACAAAA
AATTCATCGACGAACACCTGACCAACATGTCTCTGGACTGGAACTCTCTGAAACAGATCT
CTGAAAAATACTACAAATCTCGTGAAGAAAAAGACAAAAAAGTTTTCCTGTCTGAACAGA
AACGTATGCGTCAGGAAATCGTTTCTGAATTCAAAAAAGACGACCGTTTCAAAGACCTGT
TCTCTAAAAAACTGTTCTCTGAACTGCTGAAAGAAGAAATCTACAAAAAAGGTAACCACC
AGGAAATCGACGCGCTGAAATCTTTCGACAAATTCTCTGGTTACTTCATCGGTCTGCACG
AAAACCGTAAAAACATGTACTCTGACGGTGACGAAATCACCGCGATCTCTAACCGTATCG
TTAACGAAAACTTCCCGAAATTCCTGGACAACCTGCAGAAATACCAGGAAGCGCGTAAAA
AATACCCGGAATGGATCATCAAAGCGGAATCTGCGCTGGTTGCGCACAACATCAAAATGG
ACGAAGTTTTCTCTCTGGAATACTTCAACAAAGTTCTGAACCAGGAAGGTATCCAGCGTT
ACAACCTGGCGCTGGGTGGTTACGTTACCAAATCTGGTGAAAAAATGATGGGTCTGAACG
ACGCGCTGAACCTGGCGCACCAGTCTGAAAAATCTTCTAAAGGTCGTATCCACATGACCC
CGCTGTTCAAACAGATCCTGTCTGAAAAAGAATCTTTCTCTTACATCCCGGACGTTTTCA
CCGAAGACTCTCAGCTGCTGCCGTCTATCGGTGGTTTCTTCGCGCAGATCGAAAACGACA
AAGACGGTAACATCTTCGACCGTGCGCTGGAACTGATCTCTTCTTACGCGGAATACGACA
CCGAACGTATCTACATCCGTCAGGCGGACATCAACCGTGTTTCTAACGTTATCTTCGGTG
AATGGGGTACCCTGGGTGGTCTGATGCGTGAATACAAAGCGGACTCTATCAACGACATCA
ACCTGGAACGTACCTGCAAAAAAGTTGACAAATGGCTGGACTCTAAAGAATTCGCGCTGT
CTGACGTTCTGGAAGCGATCAAACGTACCGGTAACAACGACGCGTTCAACGAATACATCT
CTAAAATGCGTACCGCGCGTGAAAAAATCGACGCGGCGCGTAAAGAAATGAAATTCATCT
CTGAAAAAATCTCTGGTGACGAAGAATCTATCCACATCATCAAAACCCTGCTGGACTCTG
TTCAGCAGTTCCTGCACTTCTTCAACCTGTTCAAAGCGCGTCAGGACATCCCGCTGGACG
GTGCGTTCTACGCGGAATTCGACGAAGTTCACTCTAAACTGTTCGCGATCGTTCCGCTGT
ACAACAAAGTTCGTAACTACCTGACCAAAAACAACCTGAACACCAAAAAAATCAAACTGA
ACTTCAAAAACCCGACCCTGGCGAACGGTTGGGACCAGAACAAAGTTTACGACTACGCGT
CTCTGATCTTCCTGCGTGACGGTAACTACTACCTGGGTATCATCAACCCGAAACGTAAAA
AAAACATCAAATTCGAACAGGGTTCTGGTAACGGTCCGTTCTACCGTAAAATGGTTTACA
AACAGATCCCGGGTCCGAACAAAAACCTGCCGCGTGTTTTCCTGACCTCTACCAAAGGTA
AAAAAGAATACAAACCGTCTAAAGAAATCATCGAAGGTTACGAAGCGGACAAACACATCC
GTGGTGACAAATTCGACCTGGACTTCTGCCACAAACTGATCGACTTCTTCAAAGAATCTA
TCGAAAAACACAAAGACTGGTCTAAATTCAACTTCTACTTCTCTCCGACCGAATCTTACG
GTGACATCTCTGAATTCTACCTGGACGTTGAAAAACAGGGTTACCGTATGCACTTCGAAA
ACATCTCTGCGGAAACCATCGACGAATACGTTGAAAAAGGTGACCTGTTCCTGTTCCAGA
TCTACAACAAAGACTTCGTTAAAGCGGCGACCGGTAAAAAAGACATGCACACCATCTACT
GGAACGCGGCGTTCTCTCCGGAAAACCTGCAGGACGTTGTTGTTAAACTGAACGGTGAAG
CGGAACTGTTCTACCGTGACAAATCTGACATCAAAGAAATCGTTCACCGTGAAGGTGAAA
TCCTGGTTAACCGTACCTACAACGGTCGTACCCCGGTTCCGGACAAAATCCACAAAAAAC
TGACCGACTACCACAACGGTCGTACCAAAGACCTGGGTGAAGCGAAAGAATACCTGGACA
AAGTTCGTTACTTCAAAGCGCACTACGACATCACCAAAGACCGTCGTTACCTGAACGACA
AAATCTACTTCCACGTTCCGCTGACCCTGAACTTCAAAGCGAACGGTAAAAAAAACCTGA
ACAAAATGGTTATCGAAAAATTCCTGTCTGACGAAAAAGCGCACATCATCGGTATCGACC
GTGGTGAACGTAACCTGCTGTACTACTCTATCATCGACCGTTCTGGTAAAATCATCGACC
AGCAGTCTCTGAACGTTATCGACGGTTTCGACTACCGTGAAAAACTGAACCAGCGTGAAA
TCGAAATGAAAGACGCGCGTCAGTCTTGGAACGCGATCGGTAAAATCAAAGACCTGAAAG
AAGGTTACCTGTCTAAAGCGGTTCACGAAATCACCAAAATGGCGATCCAGTACAACGCGA
TCGTTGTTATGGAAGAACTGAACTACGGTTTCAAACGTGGTCGTTTCAAAGTTGAAAAAC
AGATCTACCAGAAATTCGAAAACATGCTGATCGACAAAATGAACTACCTGGTTTTCAAAG
ACGCGCCGGACGAATCTCCGGGTGGTGTTCTGAACGCGTACCAGCTGACCAACCCGCTGG
AATCTTTCGCGAAACTGGGTAAACAGACCGGTATCCTGTTCTACGTTCCGGCGGCGTACA
CCTCTAAAATCGACCCGACCACCGGTTTCGTTAACCTGTTCAACACCTCTTCTAAAACCA
ACGCGCAGGAACGTAAAGAATTCCTGCAGAAATTCGAATCTATCTCTTACTCTGCGAAAG
ACGGTGGTATCTTCGCGTTCGCGTTCGACTACCGTAAATTCGGTACCTCTAAAACCGACC
ACAAAAACGTTTGGACCGCGTACACCAACGGTGAACGTATGCGTTACATCAAAGAAAAAA
AACGTAACGAACTGTTCGACCCGTCTAAAGAAATCAAAGAAGCGCTGACCTCTTCTGGTA
TCAAATACGACGGTGGTCAGAACATCCTGCCGGACATCCTGCGTTCTAACAACAACGGTC
TGATCTACACCATGTACTCTTCTTTCATCGCGGCGATCCAGATGCGTGTTTACGACGGTA
AAGAAGACTACATCATCTCTCCGATCAAAAACTCTAAAGGTGAATTCTTCCGTACCGACC
CGAAACGTCGTGAACTGCCGATCGACGCGGACGCGAACGGTGCGTACAACATCGCGCTGC
GTGGTGAACTGACCATGCGTGCGATCGCGGAAAAATTCGACCCGGACTCTGAAAAAATGG
CGAAACTGGAACTGAAACACAAAGACTGGTTCGAATTCATGCAGACCCGTGGTGACTAAG
AAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATGTAGGGAGACCCTCAGG
TTAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACA
SEQ AAAATTCcatGCAAAATGCTCCGGTTTCATGTCATCAAAATGATGACGTAATTAAGCATT
ID GATAATTGAGATCCCTCTCCCTGACAGGATGATTACATAAATAATAGTGACAAAAATAAA
NO: TTATTTATTTATCCAGAAAATGAATTGGAAAATCAGGAGAGCGTTTTCAATCCTACCTCT
64 GGCGCAGTTGATATGTcaaaCAGGTtgccgtcactgcgtcttttactggctcttctcgct
aaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccat
gacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattattt
gcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctac
ctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagcac
cgcctatctcgtgtgagataggcggagatacgaactttaagAAGGAGatataccATGACT
AAAACATTTGATTCAGAGTTTTTTAATTTGTACTCGCTGCAAAAAACGGTACGCTTTGAG
TTAAAACCCGTGGGAGAAACCGCGTCATTTGTGGAAGACTTTAAAAACGAGGGCTTGAAA
CGTGTTGTGAGCGAAGATGAAAGGCGAGCCGTCGATTACCAGAAAGTTAAGGAAATAATT
GACGATTACCATCGGGATTTCATTGAAGAAAGTTTAAATTATTTTCCGGAACAGGTGAGT
AAAGATGCTCTTGAGCAGGCGTTTCATCTTTATCAGAAACTGAAGGCAGCAAAAGTTGAG
GAAAGGGAAAAAGCGCTGAAAGAATGGGAAGCGCTGCAGAAAAAGCTACGTGAAAAAGTG
GTGAAATGCTTCTCGGACTCGAATAAAGCCCGCTTCTCAAGGATTGATAAAAAGGAACTG
ATTAAGGAAGACCTGATAAATTGGTTGGTCGCCCAGAATCGCGAGGATGATATCCCTACG
GTCGAAACGTTTAACAACTTCACCACATATTTTACCGGCTTCCATGAGAATCGTAAAAAT
ATTTACTCCAAAGATGATCACGCCACCGCTATTAGCTTTCGCCTTATTCATGAAAATCTT
CCAAAGTTTTTTGACAACGTGATTAGCTTCAATAAGTTGAAAGAGGGTTTCCCTGAATTA
AAATTTGATAAAGTGAAAGAGGATTTAGAAGTAGATTATGATCTGAAGCATGCGTTTGAA
ATAGAATATTTCGTTAACTTCGTGACCCAAGCGGGCATAGATCAGTATAATTATCTGTTA
GGAGGGAAAACCCTGGAGGACGGGACGAAAAAACAAGGGATGAATGAGCAAATTAATCTG
TTCAAACAACAGCAAACGCGAGATAAAGCGCGTCAGATTCCCAAACTGATCCCCCTGTTC
AAACAGATTCTTAGCGAAAGGACTGAAAGCCAGTCCTTTATTCCTAAACAATTTGAAAGT
GATCAGGAGTTGTTCGATTCACTGCAGAAGTTACATAATAACTGCCAGGATAAATTCACC
GTGCTGCAACAAGCCATTCTCGGTCTGGCAGAGGCGGATCTTAAGAAGGTCTTCATCAAA
ACCTCTGATTTAAATGCCTTATCTAACACCATTTTCGGGAATTACAGCGTCTTTTCCGAT
GCACTGAACCTGTATAAAGAAAGCCTGAAAACGAAAAAAGCGCAGGAGGCTTTTGAGAAA
CTACCGGCCCATTCTATTCACGACCTCATTCAATACTTGGAACAGTTCAATTCCAGCCTG
GACGCGGAAAAACAACAGAGCACCGACACCGTCCTGAACTACTTCATCAAGACCGATGAA
TTATATTCTCGCTTCATTAAATCCACTAGCGAGGCTTTCACTCAGGTGCAGCCTTTGTTC
GAACTGGAAGCCCTGTCATCTAAGCGCCGCCCACCGGAATCGGAAGATGAAGGGGCAAAA
GGGCAGGAAGGCTTCGAGCAGATCAAGCGTATTAAAGCTTACCTGGATACGCTTATGGAA
GCGGTACACTTTGCAAAGCCGTTGTATCTTGTTAAGGGTCGTAAAATGATCGAAGGGCTC
GATAAAGACCAGTCCTTTTATGAAGCGTTTGAAATGGCGTACCAAGAACTTGAATCGTTA
ATCATTCCTATCTATAACAAAGCGCGGAGCTATCTGTCGCGGAAACCTTTCAAGGCCGAT
AAATTCAAGATTAATTTTGACAACAACACGCTACTGAGCGGATGGGATGCGAACAAGGAA
ACTGCTAACGCGTCCATTCTGTTTAAGAAAGACGGGTTATATTACCTTGGAATTATGCCG
AAAGGTAAGACCTTTCTCTTTGACTACTTTGTATCGAGCGAGGATTCAGAGAAACTGAAA
CAGCGTCGCCAGAAGACCGCCGAAGAAGCTCTGGCGCAGGATGGTGAAAGTTACTTCGAA
AAAATTCGTTATAAACTGTTACCAGGGGCTTCAAAGATGTTACCGAAAGTCTTTTTTAGC
AACAAAAATATTGGCTTTTACAACCCGTCGGATGACATTTTACGCATTCGCAACACAGCC
TCTCACACCAAAAACGGGACCCCTCAGAAAGGCCACTCAAAAGTTGAGTTTAACCTGAAT
GATTGTCATAAGATGATTGATTTCTTCAAATCATCAATTCAGAAACACCCGGAATGGGGG
TCTTTTGGCTTTACGTTTTCTGATACCAGTGATTTTGAAGACATGAGTGCCTTCTACCGG
GAAGTAGAAAACCAGGGTTACGTAATTAGCTTTGACAAAATCAAAGAGACCTATATACAG
AGCCAGGTGGAACAGGGTAATCTCTACTTATTCCAGATTTATAACAAGGATTTCTCGCCC
TACAGCAAAGGCAAACCAAACCTGCATACTCTGTACTGGAAAGCCCTGTTTGAAGAAGCG
AACCTGAATAACGTAGTGGCGAAGTTGAACGGTGAAGCGGAAATCTTCTTCCGTCGTCAC
TCCATTAAGGCCTCTGATAAAGTTGTCCATCCGGCAAATCAGGCCATTGATAATAAGAAT
CCACACACGGAAAAAACGCAGTCAACCTTTGAATATGACCTCGTTAAAGACAAACGCTAC
ACGCAAGATAAGTTCTTTTTCCACGTCCCAATCAGCCTCAACTTTAAAGCACAAGGGGTT
TCAAAGTTTAATGATAAAGTCAATGGGTTCCTCAAGGGCAACCCGGATGTCAACATTATA
GGTATAGACAGGGGCGAACGCCATCTGCTTTACTTTACCGTAGTGAATCAGAAAGGTGAA
ATACTGGTTCAGGAATCATTAAATACCTTGATGTCGGACAAAGGGCACGTTAATGATTAC
CAGCAGAAACTGGATAAAAAAGAACAGGAACGTGATGCTGCGCGTAAATCGTGGACCACG
GTTGAGAACATTAAAGAGCTGAAAGAGGGGTATCTAAGCCATGTGGTACACAAACTGGCG
CACCTCATCATTAAATATAACGCAATAGTCTGCCTAGAAGACTTGAATTTTGGCTTTAAA
CGCGGCCGCTTCAAAGTGGAAAAACAAGTTTATCAAAAATTTGAAAAGGCGCTTATAGAT
AAACTGAATTATCTGGTTTTTAAAGAAAAGGAACTTGGTGAGGTAGGGCACTACTTGACA
GCTTATCAACTGACGGCCCCGTTCGAATCATTCAAAAAACTGGGCAAACAGTCTGGCATT
CTGTTTTACGTGCCGGCAGATTATACTTCAAAAATCGATCCAACAACTGGCTTTGTGAAC
TTCCTGGACCTGAGATATCAGTCTGTAGAAAAAGCTAAACAACTTCTTAGCGATTTTAAT
GCCATTCGTTTTAACAGCGTTCAGAATTACTTTGAATTCGAAATTGACTATAAAAAACTT
ACTCCGAAACGTAAAGTCGGAACCCAAAGTAAATGGGTAATTTGTACGTATGGCGATGTC
AGGTATCAGAACCGTCGGAATCAAAAAGGTCATTGGGAGACCGAAGAAGTGAACGTGACC
GAAAAGCTGAAGGCTCTGTTCGCCAGCGATTCAAAAACTACAACTGTGATCGATTACGCA
AATGATGATAACCTGATAGATGTGATTTTAGAGCAGGATAAAGCCAGCTTTTTTAAAGAA
CTGTTGTGGCTCCTGAAACTTACGATGACCTTACGACATTCCAAGATCAAATCGGAAGAT
GATTTTATTCTGTCACCGGTCAAGAATGAGCAGGGTGAATTCTATGATAGTAGGAAAGCC
GGCGAAGTGTGGCCGAAAGACGCCGACGCCAATGGCGCCTATCATATCGCGCTCAAAGGG
CTTTGGAATTTGCAGCAGATTAACCAGTGGGAAAAAGGTAAAACCCTGAATCTGGCTATC
AAAAACCAGGATTGGTTTAGCTTTATCCAAGAGAAACCGTATCAGGAATGAGAAATCATC
CTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATTTATTATATCGCGTTGATTATTGAT
GCTGTTTTTAGTTTTAACGGCAATTAATATATGTGTTATTAATTGAATGAATTTTATCAT
TCATAATAAGTATGTGTAGGATCAAGCTCAGGTTAAATATTCACTCAGGAAGTTATTACT
CAGGAAGCAAAGAGGATTACAGAATTATCTCATAACAAGTGTTAAGGGATGTTATTTCC
SEQ AATCAGGAGAGCGTTTTCAATCCTACCTCTGGCGCAGTTGATATGTCAAACAGGTTGCCG
ID TCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAG
NO: CATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAA
65 TCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC
ATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGT
TTCTCCATACCCGTTTTTTTGGGCTAGCAGTAATACGACTCACTATAGGGGTCTCATCTC
GTGTGAGATAGGCGGAGATACGAACTTTAAGAGGAGGATATACCATGCACCATCATCATC
ACCATCATACAGGCGGTCTTCTTAGTATGGACGCGAAAGAGTTCACAGGTCAGTATCCGT
TGTCGAAAACATTACGATTCGAACTTCGGCCCATCGGCCGCACGTGGGATAACCTGGAGG
CCTCAGGCTACTTAGCGGAAGACCGCCATCGTGCCGAATGTTATCCTCGTGCGAAAGAGT
TATTGGATGACAACCATCGTGCCTTCCTGAATCGTGTGTTGCCACAAATCGATATGGATT
GGCACCCGATTGCGGAGGCCTTTTGTAAGGTACATAAAAACCCTGGTAATAAAGAACTTG
CCCAGGATTACAACCTTCAGTTGTCAAAGCGCCGTAAGGAGATCAGCGCATATCTTCAGG
ATGCAGATGGCTATAAAGGCCTGTTCGCGAAGCCCGCCTTAGACGAAGCTATGAAAATTG
CGAAAGAAAACGGGAACGAAAGTGATATTGAGGTTCTCGAAGCGTTTAACGGTTTTAGCG
TATACTTCACCGGTTATCATGAGTCACGCGAGAACATTTATAGCGATGAGGATATGGTGA
GCGTAGCCTACCGAATTACTGAGGATAATTTCCCGCGCTTTGTCTCAAACGCTTTGATCT
TTGATAAATTAAACGAAAGCCATCCGGATATTATCTCTGAAGTATCGGGCAATCTTGGAG
TTGATGACATTGGTAAGTACTTTGACGTGTCGAACTATAACAATTTTCTTTCCCAGGCCG
GTATAGATGACTACAATCACATTATTGGCGGCCATACAACCGAAGACGGACTGATACAAG
CGTTTAATGTCGTATTGAACTTACGTCACCAAAAAGACCCTGGCTTTGAAAAAATTCAGT
TCAAACAGCTCTACAAACAAATCCTGAGCGTGCGTACCAGCAAAAGCTACATCCCGAAAC
AGTTTGACAACTCTAAGGAGATGGTTGACTGCATTTGCGATTATGTCAGCAAAATAGAGA
AATCCGAAACAGTAGAACGGGCCCTGAAACTAGTCCGTAATATCAGTTCTTTCGACTTGC
GCGGGATCTTTGTCAATAAAAAGAACTTGCGCATACTGAGCAACAAACTGATAGGAGATT
GGGACGCGATCGAAACCGCATTGATGCATAGTTCTTCATCAGAAAACGATAAGAAAAGCG
TATATGATAGCGCGGAGGCTTTTACGTTGGATGACATCTTTTCAAGCGTGAAAAAATTTT
CTGATGCCTCTGCCGAAGATATTGGCAACAGGGCGGAAGACATCTGTAGAGTGATAAGTG
AGACGGCCCCTTTTATCAACGATCTGCGAGCGGTGGACCTGGATAGCCTGAACGACGATG
GTTATGAAGCGGCCGTCTCAAAAATTCGGGAGTCGCTGGAGCCTTATATGGATCTTTTCC
ATGAACTGGAAATTTTCTCGGTTGGCGATGAGTTCCCAAAATGCGCAGCATTTTACAGCG
AACTGGAGGAAGTCAGCGAACAGCTGATCGAAATTATTCCGTTATTCAACAAGGCGCGTT
CGTTCTGCACCCGGAAACGCTATAGCACCGATAAGATTAAAGTGAACTTAAAATTCCCGA
CCTTGGCGGACGGGTGGGACCTGAACAAAGAGAGAGACAACAAAGCCGCGATTCTGCGGA
AAGACGGTAAGTATTATCTGGCAATTCTGGATATGAAGAAAGATCTGTCAAGCATTAGGA
CCAGCGACGAAGATGAATCCAGCTTCGAAAAGATGGAGTATAAACTGTTACCGAGTCCAG
TAAAAATGCTGCCAAAGATATTCGTAAAATCGAAAGCCGCTAAGGAAAAATATGGCCTGA
CAGATCGTATGCTTGAATGCTACGATAAAGGTATGCATAAGTCGGGTAGTGCGTTTGATC
TTGGCTTTTGCCATGAACTCATTGATTATTACAAGCGTTGTATCGCGGAGTACCCAGGCT
GGGATGTGTTCGATTTCAAGTTTCGCGAAACTTCCGATTATGGGTCCATGAAAGAGTTCA
ATGAAGATGTGGCCGGAGCCGGTTACTATATGAGTCTGAGAAAAATTCCGTGCAGCGAAG
TGTACCGTCTGTTAGACGAGAAATCGATTTATCTATTTCAAATTTATAACAAAGATTACT
CTGAAAATGCACATGGTAATAAGAACATGCATACCATGTACTGGGAGGGTCTCTTTTCCC
CGCAAAACCTGGAGTCGCCCGTTTTCAAGTTGTCGGGTGGGGCAGAACTTTTCTTTCGAA
AATCCTCAATCCCTAACGATGCCAAAACAGTACACCCGAAAGGCTCAGTGCTGGTTCCAC
GTAATGATGTTAACGGTCGGCGTATTCCAGATTCAATCTACCGCGAACTGACACGCTATT
TTAACCGTGGCGATTGCCGAATCAGTGACGAAGCCAAAAGTTATCTTGACAAGGTTAAGA
CTAAAAAAGCGGACCATGACATTGTGAAAGATCGCCGCTTTACCGTGGATAAAATGATGT
TCCACGTCCCGATTGCGATGAACTTTAAGGCGATCAGTAAACCGAACTTAAACAAAAAAG
TCATTGATGGCATCATTGATGATCAGGATCTGAAAATCATTGGTATTGATCGTGGCGAGC
GGAACTTAATTTACGTCACGATGGTTGACAGAAAAGGGAATATCTTATATCAGGATTCTC
TTAACATCCTCAATGGCTACGACTATCGTAAAGCTCTGGATGTGCGCGAATATGACAACA
AGGAAGCGCGTCGTAACTGGACTAAAGTGGAGGGCATTCGCAAAATGAAGGAAGGCTATC
TGTCATTAGCGGTCTCGAAATTAGCGGATATGATTATCGAAAATAACGCCATCATCGTTA
TGGAGGACCTGAACCACGGATTCAAAGCGGGCCGCTCAAAGATTGAAAAACAAGTTTATC
AGAAATTTGAGAGTATGCTGATTAACAAACTGGGCTATATGGTGTTAAAAGACAAGTCAA
TTGACCAATCAGGTGGCGCGCTGCATGGATACCAGCTGGCGAACCATGTTACCACCTTAG
CATCAGTTGGAAAGCAGTGTGGGGTTATCTTTTATATACCGGCAGCGTTCACTAGTAAAA
TAGATCCGACCACTGGTTTCGCCGATCTCTTTGCCCTGAGTAACGTTAAAAACGTAGCGA
GCATGCGTGAATTCTTTTCCAAAATGAAATCTGTCATTTATGATAAAGCTGAAGGCAAAT
TCGCATTCACCTTTGATTACTTGGATTACAACGTGAAGAGCGAATGTGGTCGTACGCTGT
GGACCGTTTACACCGTTGGTGAGCGCTTCACCTATTCCCGTGTGAACCGCGAATATGTAC
GTAAAGTCCCCACCGATATTATCTATGATGCCCTCCAGAAAGCAGGCATTAGCGTCGAAG
GAGACTTAAGGGACAGAATTGCCGAAAGCGATGGCGATACGCTGAAGTCTATTTTTTACG
CATTCAAATACGCGCTAGATATGCGCGTTGAGAATCGCGAGGAAGACTACATTCAATCAC
CTGTGAAAAATGCCTCTGGGGAATTTTTTTGTTCAAAAAATGCTGGTAAAAGCCTCCCAC
AAGATAGCGATGCAAACGGTGCATATAACATTGCCCTGAAAGGTATTCTTCAATTACGCA
TGCTGTCTGAGCAGTACGACCCCAACGCGGAATCTATTAGACTTCCGCTGATAACCAATA
AAGCCTGGCTGACATTCATGCAGTCTGGCATGAAGACCTGGAAAAATTAGGAAATCATCC
TTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATGTAGGGAGACCCTCAGGTTAAATATT
CACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACA
SEQ AAAATTCcatGCAAAATGCTCCGGTTTCATGTCATCAAAATGATGACGTAATTAAGCATT
ID GATAATTGAGATCCCTCTCCCTGACAGGATGATTACATAAATAATAGTGACAAAAATAAA
NO: TTATTTATTTATCCAGAAAATGAATTGGAAAATCAGGAGAGCGTTTTCAATCCTACCTCT
66 GGCGCAGTTGATATGTcaaaCAGGTtgccgtcactgcgtcttttactggctcttctcgct
aaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccat
gacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattattt
gcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctac
ctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagcac
cgcctatctcgtgtgagataggcggagatacgaactttaagAAGGAGatataccatgGAT
AGTTTGAAAGATTTCACCAATCTGTACCCTGTCAGTAAGACATTGAGATTTGAATTAAAG
CCCGTTGGAAAGACTTTAGAAAATATCGAGAAAGCAGGTATTTTGAAAGAGGATGAGCAT
CGTGCAGAAAGTTATCGGAGGGTGAAGAAAATAATTGATACTTATCATAAGGTATTTATC
GATTCTTCTCTTGAAAATATGGCTAAAATGGGTATTGAGAATGAAATAAAAGCAATGCTC
CAAAGTTTCTGCGAATTGTATAAAAAAGATCATCGCACTGAGGGTGAAGACAAGGCATTA
GATAAAATTCGAGCAGTACTTCGTGGCCTGATTGTTGGGGCTTTCACTGGTGTTTGCGGA
AGACGGGAAAATACAGTCCAAAACGAGAAGTACGAGAGTTTGTTCAAAGAAAAGTTGATA
AAAGAAATTTTACCTGATTTTGTGCTCTCTACTGAGGCTGAAAGCTTGCCTTTCTCTGTT
GAAGAAGCTACGAGGTCACTGAAGGAGTTTGATAGCTTTACATCCTACTTTGCTGGTTTT
TACGAGAATAGAAAGAATATATACTCGACGAAACCTCAATCCACTGCCATTGCTTATCGT
CTTATTCATGAGAACTTGCCGAAGTTCATTGATAATATTCTTGTTTTTCAGAAGATCAAA
GAGCCTATAGCCAAAGAGCTGGAACATATTCGTGCGGACTTTTCTGCCGGGGGGTACATA
AAAAAGGATGAGAGATTGGAGGATATTTTTTCGTTGAACTATTATATCCACGTGTTATCT
CAGGCTGGGATCGAAAAATATAACGCATTGATTGGGAAGATTGTGACAGAAGGAGATGGA
GAGATGAAAGGGCTCAATGAACACATCAACCTTTACAACCAACAAAGAGGCAGAGAGGAT
CGGCTCCCTCTTTTTAGGCCTCTTTATAAACAGATATTGAGTGACAGAGAGCAATTATCA
TACTTGCCTGAGAGTtttGAAAAAGAtGAGGAGctcctcAGGGctctAAAAGAGttctAt
GAtcAtAtcGcAGAAGACATTCTCGGACGTACTCAACAGTTGATGACTTCTATTTCAGAA
TATGATTTATCTCGGATATACGTAAGGAACGATAGCCAATTGACTGATATATCAAAAAAA
ATGTTGGGAGATTGGAATGCTATCTACATGGCTAGAGAACGAGCATATGACCACGAGCAG
GCTCCCAAAAGAATCACGGCGAAATACGAGAGGGACAGGATTAAAGCTCTTAAAGGAGAA
GAGAGTATAAGTCTGGCAAATCTTAATAGTTGTATTGCCTTTCTGGACAATGTTAGAGAT
TGCCGTGTAGATACTTATCTTTCCACACTGGGCCAGAAGGAAGGACCACATGGTCTATCT
AATCTCGTTGAGAACGTTTTTGCCTCATACCATGAAGCAGAGCAATTGTTGAGCTTTCCA
TACCCCGAAGAGAATAATCTGATTCAGGACAAGGACAATGTGGTGTTAATTAAGAATCTT
CTCGACAATATCAGTGATCTGCAGAGGTTCTTGAAACCTCTTTGGGGTATGGGAGACGAA
CCCGATAAAGATGAAAGATTTTATGGAGAGTATAATTATATCCGAGGAGCTCTAGATCAG
GTGATCCCTCTGTACAATAAGGTAAGGAACTACCTCACTCGGAAGCCTTATTCGACCAGA
AAAGTAAAACTCAATTTTGGGAATTCTCAATTGCTTAGTGGTTGGGATAGAAATAAGGAA
AAGGATAATAGCTGTGTGATTTTGCGTAAGGGGCAGAACTTCTATTTGGCTATTATGAAC
AATAGGCACAAAAGAAGTTTCGAAAACAAGGTGTTGCCCGAGTATAAGGAGGGAGAACCT
TACTTCGAAAAGATGGATTATAAATTTTTGCCTGATCCTAATAAAATGCTTCCTAAGGTT
TTTCTTTCGAAAAAAGGAATAGAGATATACAAACCAAGTCCGAAGCTTTTAGAACAATAT
GGACATGGAACTCACAAAAAGGGAGATACCTTTAGTATGGATGATTTGCACGAACTGATC
GATTTCTTCAAACACTCAATCGAGGCTCATGAAGATTGGAAGCAATTCGGATTCAAATTT
TCTGATACGGCTACTTATGAGAATGTATCTAGTTTCTATAGAGAAGTTGAGGATCAGGGG
TATAAGCTCTCTTTCCGAAAAGTTTCGGAATCTTATGTCTATTCATTAATAGATCAAGGC
AAGTTGTATTTATTTCAGATATACAACAAGGACTTTTCTCCCTGCAGCAAAGGGACACCT
AATCTGCATACCTTGTATTGGAGAATGCTTTTTGACGAGCGCAATTTGGCAGATGTCATA
TACAAACTGGATGGGAAGGCTGAAATCTTTTTCCGAGAGAAGAGTTTGAAAAATGATCAT
CCCACGCATCCGGCTGGTAAGCCTATCAAAAAGAAAAGTCGACAAAAAAAAGGAGAGGAG
AGTCTGTTTGAGTATGATTTAGTCAAGGATAGGCACTATACGATGGATAAGTTCCAGTTT
CATGTGCCTATTACTATGAATTTTAAATGTTCTGCAGGAAGCAAAGTCAATGATATGGTT
AATGCTCATATTCGAGAGGCAAAGGATATGCATGTCATTGGAATTGATCGTGGAGAACGC
AATCTGCTGTATATATGCGTGATAGATAGTCGAGGGACGATTTTGGATCAAATTTCTCTG
AATACGATTAACGATATAGACTATCATGATTTATTGGAGAGTCGAGACAAAGACCGTCAG
CAGGAGCGCCGAAACTGGCAAACTATCGAAGGGATCAAGGAGCTAAAACAAGGCTACCTT
AGTCAGGCGGTTCATCGGATAGCCGAACTGATGGTGGCTTATAAGGCTGTAGTTGCTTTG
GAGGATTTGAATATGGGGTTCAAACGTGGGCGGCAGAAAGTAGAAAGTTCTGTTTATCAG
CAGTTTGAGAAACAGCTGATAGATAAGCTCAACTATCTTGTGGACAAGAAGAAAAGGCCT
GAAGATATTGGAGGATTGTTGAGAGCCTATCAATTTACGGCCCCATTTAAGAGTTTTAAG
GAAATGGGAAAGCAAAACGGCTTCTTGTTTTATATCCCGGCTTGGAACACGAGCAACATA
GATCCGACTACTGGATTTGTTAATTTATTTCATGCCCAGTATGAAAATGTAGATAAAGCG
AAGAGCTTCTTTCAAAAGTTTGATTCAATTAGTTACAACCCGAAGAAAGACTGGTTTGAG
TTTGCATTCGATTATAAAAACTTTACTAAAAAGGCTGAAGGAAGTCGTTCTATGTGGATA
TTATGCACACATGGTTCCCGAATAAAGAATTTTAGAAATTCCCAGAAGAATGGTCAATGG
GATTCCGAAGAATTCGCCTTGACGGAGGCTTTTAAGTCTCTTTTTGTGCGATATGAGATA
GATTATACCGCTGATTTGAAAACAGCTATTGTGGACGAAAAGCAAAAAGACTTCTTCGTG
GATCTTCTGAAGCTATTCAAATTGACAGTACAGATGCGCAACAGCTGGAAAGAGAAGGAT
TTGGATTATCTAATCTCTCCTGTAGCAGGGGCTGATGGCCGTTTCTTCGATACAAGAGAG
GGAAATAAAAGTCTGCCTAAGGATGCAGATGCCAATGGAGCTTATAATATTGCCCTAAAA
GGACTTTGGGCTCTACGCCAGATTCGGCAAACTTCAGAAGGCGGTAAACTCAAATTGGCG
ATTTCCAATAAGGAATGGCTACAGTTTGTGCAAGAGAGATCTTACGAGAAAGACtgaGAA
ATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATTTATTATATCGCGTTGATT
ATTGATGCTGTTTTTAGTTTTAACGGCAATTAATATATGTGTTATTAATTGAATGAATTT
TATCATTCATAATAAGTATGTGTAGGATCAAGCTCAGGTTAAATATTCACTCAGGAAGTT
ATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATAACAAGTGTTAAGGGATGTTA
TTTCC
SEQ AAAATTCcatGCAAAATGCTCCGGTTTCATGTCATCAAAATGATGACGTAATTAAGCATT
ID GATAATTGAGATCCCTCTCCCTGACAGGATGATTACATAAATAATAGTGACAAAAATAAA
NO: TTATTTATTTATCCAGAAAATGAATTGGAAAATCAGGAGAGCGTTTTCAATCCTACCTCT
67 GGCGCAGTTGATATGTcaaaCAGGTtgccgtcactgcgtcttttactggctcttctcgct
aaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccat
gacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattattt
gcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctac
ctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagcac
cgcctatctcgtgtgagataggcggagatacgaactttaagAAGGAGatataccATGAAC
AACGGCACAAATAATTTTCAGAACTTCATCGGGATCTCAAGTTTGCAGAAAACGCTGCGC
AATGCTCTGATCCCCACGGAAACCACGCAACAGTTCATCGTCAAGAACGGAATAATTAAA
GAAGATGAGTTACGTGGCGAGAACCGCCAGATTCTGAAAGATATCATGGATGACTACTAC
CGCGGATTCATCTCTGAGACTCTGAGTTCTATTGATGACATAGATTGGACTAGCCTGTTC
GAAAAAATGGAAATTCAGCTGAAAAATGGTGATAATAAAGATACCTTAATTAAGGAACAG
ACAGAGTATCGGAAAGCAATCCATAAAAAATTTGCGAACGACGATCGGTTTAAGAACATG
TTTAGCGCCAAACTGATTAGTGACATATTACCTGAATTTGTCATCCACAACAATAATTAT
TCGGCATCAGAGAAAGAGGAAAAAACCCAGGTGATAAAATTGTTTTCGCGCTTTGCGACT
AGCTTTAAAGATTACTTCAAGAACCGTGCAAATTGCTTTTCAGCGGACGATATTTCATCA
AGCAGCTGCCATCGCATCGTCAACGACAATGCAGAGATATTCTTTTCAAATGCGCTGGTC
TACCGCCGGATCGTAAAATCGCTGAGCAATGACGATATCAACAAAATTTCGGGCGATATG
AAAGATTCATTAAAAGAAATGAGTCTGGAAGAAATATATTCTTACGAGAAGTATGGGGAA
TTTATTACCCAGGAAGGCATTAGCTTCTATAATGATATCTGTGGGAAAGTGAATTCTTTT
ATGAACCTGTATTGTCAGAAAAATAAAGAAAACAAAAATTTATACAAACTTCAGAAACTT
CACAAACAGATTCTATGCATTGCGGACACTAGCTATGAGGTCCCGTATAAATTTGAAAGT
GACGAGGAAGTGTACCAATCAGTTAACGGCTTCCTTGATAACATTAGCAGCAAACATATA
GTCGAAAGATTACGCAAAATCGGCGATAACTATAACGGCTACAACCTGGATAAAATTTAT
ATCGTGTCCAAATTTTACGAGAGCGTTAGCCAAAAAACCTACCGCGACTGGGAAACAATT
AATACCGCCCTCGAAATTCATTACAATAATATCTTGCCGGGTAACGGTAAAAGTAAAGCC
GACAAAGTAAAAAAAGCGGTTAAGAATGATTTACAGAAATCCATCACCGAAATAAATGAA
CTAGTGTCAAACTATAAGCTGTGCAGTGACGACAACATCAAAGCGGAGACTTATATACAT
GAGATTAGCCATATCTTGAATAACTTTGAAGCACAGGAATTGAAATACAATCCGGAAATT
CACCTAGTTGAATCCGAGCTCAAAGCGAGTGAGCTTAAAAACGTGCTGGACGTGATCATG
AATGCGTTTCATTGGTGTTCGGTTTTTATGACTGAGGAACTTGTTGATAAAGACAACAAT
TTTTATGCGGAACTGGAGGAGATTTACGATGAAATTTATCCAGTAATTAGTCTGTACAAC
CTGGTTCGTAACTACGTTACCCAGAAACCGTACAGCACGAAAAAGATTAAATTGAACTTT
GGAATACCGACGTTAGCAGACGGTTGGTCAAAGTCCAAAGAGTATTCTAATAACGCTATC
ATACTGATGCGCGACAATCTGTATTATCTGGGCATCTTTAATGCGAAGAATAAACCGGAC
AAGAAGATTATCGAGGGTAATACGTCAGAAAATAAGGGTGACTACAAAAAGATGATTTAT
AATTTGCTCCCGGGTCCCAACAAAATGATCCCGAAAGTTTTCTTGAGCAGCAAGACGGGG
GTGGAAACGTATAAACCGAGCGCCTATATCCTAGAGGGGTATAAACAGAATAAACATATC
AAGTCTTCAAAAGACTTTGATATCACTTTCTGTCATGATCTGATCGACTACTTCAAAAAC
TGTATTGCAATTCATCCCGAGTGGAAAAACTTCGGTTTTGATTTTAGCGACACCAGTACT
TATGAAGACATTTCCGGGTTTTATCGTGAGGTAGAGTTACAAGGTTACAAGATTGATTGG
ACATACATTAGCGAAAAAGACATTGATCTGCTGCAGGAAAAAGGTCAACTGTATCTGTTC
CAGATATATAACAAAGATTTTTCGAAAAAATCAACCGGGAATGACAACCTTCACACCATG
TACCTGAAAAATCTTTTCTCAGAAGAAAATCTTAAGGATATCGTCCTGAAACTTAACGGC
GAAGCGGAAATCTTCTTCAGGAAGAGCAGCATAAAGAACCCAATCATTCATAAAAAAGGC
TCGATTTTAGTCAACCGTACCTACGAAGCAGAAGAAAAAGACCAGTTTGGCAACATTCAA
ATTGTGCGTAAAAATATTCCGGAAAACATTTATCAGGAGCTGTACAAATACTTCAACGAT
AAAAGCGACAAAGAGCTGTCTGATGAAGCAGCCAAACTGAAGAATGTAGTGGGACACCAC
GAGGCAGCGACGAATATAGTCAAGGACTATCGCTACACGTATGATAAATACTTCCTTCAT
ATGCCTATTACGATCAATTTCAAAGCCAATAAAACGGGTTTTATTAATGATAGGATCTTA
CAGTATATCGCTAAAGAAAAAGACTTACATGTGATCGGCATTGATCGGGGCGAGCGTAAC
CTGATCTACGTGTCCGTGATTGATACTTGTGGTAATATAGTTGAACAGAAAAGCTTTAAC
ATTGTAAACGGCTACGACTATCAGATAAAACTGAAACAACAGGAGGGCGCTAGACAGATT
GCGCGGAAAGAATGGAAAGAAATTGGTAAAATTAAAGAGATCAAAGAGGGCTACCTGAGC
TTAGTAATCCACGAGATCTCTAAAATGGTAATCAAATACAATGCAATTATAGCGATGGAG
GATTTGTCTTATGGTTTTAAAAAAGGGCGCTTTAAGGTCGAACGGCAAGTTTACCAGAAA
TTTGAAACCATGCTCATCAATAAACTCAACTATCTGGTATTTAAAGATATTTCGATTACC
GAGAATGGCGGTCTCCTGAAAGGTTATCAGCTGACATACATTCCTGATAAACTTAAAAAC
GTGGGTCATCAGTGCGGCTGCATTTTTTATGTGCCTGCTGCATACACGAGCAAAATTGAT
CCGACCACCGGCTTTGTGAATATCTTTAAATTTAAAGACCTGACAGTGGACGCAAAACGT
GAATTCATTAAAAAATTTGACTCAATTCGTTATGACAGTGAAAAAAATCTGTTCTGCTTT
ACATTTGACTACAATAACTTTATTACGCAAAACACGGTCATGAGCAAATCATCGTGGAGT
GTGTATACATACGGCGTGCGCATCAAACGTCGCTTTGTGAACGGCCGCTTCTCAAACGAA
AGTGATACCATTGACATAACCAAAGATATGGAGAAAACGTTGGAAATGACGGACATTAAC
TGGCGCGATGGCCACGATCTTCGTCAAGACATTATAGATTATGAAATTGTTCAGCACATA
TTCGAAATTTTCCGTTTAACAGTGCAAATGCGTAACTCCTTGTCTGAACTGGAGGACCGT
GATTACGATCGTCTCATTTCACCTGTACTGAACGAAAATAACATTTTTTATGACAGCGCG
AAAGCGGGGGATGCACTTCCTAAGGATGCCGATGCAAATGGTGCGTATTGTATTGCATTA
AAAGGGTTATATGAAATTAAACAAATTACCGAAAATTGGAAAGAAGATGGTAAATTTTCG
CGCGATAAACTCAAAATCAGCAATAAAGATTGGTTCGACTTTATCCAGAATAAGCGCTAT
CTCTAAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATTTATTATATC
GCGTTGATTATTGATGCTGTTTTTAGTTTTAACGGCAATTAATATATGTGTTATTAATTG
AATGAATTTTATCATTCATAATAAGTATGTGTAGGATCAAGCTCAGGTTAAATATTCACT
CAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATAACAAGTGTTAA
GGGATGTTATTTCC
SEQ AATCAGGAGAGCGTTTTCAATCCTACCTCTGGCGCAGTTGATATGTCAAACAGGTTGCCG
ID TCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAG
NO: CATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAA
68 TCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC
ATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGT
TTCTCCATACCCGTTTTTTTGGGCTAGCAGTAATACGACTCACTATAGGGGTCTCATCTC
GTGTGAGATAGGCGGAGATACGAACTTTAAGAGGAGGATATACCATGCACCATCATCATC
ACCATACCAATAAATTCACTAACCAGTATTCTCTCTCTAAGACCCTGCGCTTTGAACTGA
TTCCGCAGGGGAAAACCTTGGAGTTCATTCAAGAAAAAGGCCTCTTGTCTCAGGATAAAC
AGAGGGCTGAATCTTACCAAGAAATGAAGAAAACTATTGATAAGTTTCATAAATATTTCA
TTGATTTAGCCTTGTCTAACGCCAAATTAACTCACTTGGAAACGTATCTGGAGTTATACA
ACAAATCTGCCGAAACTAAGAAAGAACAGAAATTTAAAGACGATTTGAAAAAAGTACAGG
ACAATCTGCGTAAAGAAATTGTCAAATCCTTCAGTGACGGCGATGCTAAAAGCATTTTTG
CCATTCTGGACAAAAAAGAGTTGATTACTGTGGAATTAGAAAAGTGGTTTGAAAACAATG
AGCAGAAAGACATCTACTTCGATGAGAAATTCAAAACTTTCACCACCTATTTTACAGGAT
TTCATCAAAACCGGAAGAACATGTACTCAGTAGAACCGAACTCCACGGCCATTGCGTATC
GTTTGATCCATGAGAATCTGCCTAAATTTCTGGAGAATGCGAAAGCCTTTGAAAAGATTA
AGCAGGTCGAATCGCTGCAAGTGAATTTTCGTGAACTCATGGGCGAATTTGGTGACGAAG
GTCTAATCTTCGTTAACGAACTGGAAGAAATGTTTCAGATTAATTACTACAATGACGTGC
TATCGCAGAACGGTATCACAATCTACAATAGTATTATCTCAGGGTTCACAAAAAACGATA
TAAAATACAAAGGCCTGAACGAGTATATCAATAACTACAACCAAACAAAGGACAAAAAGG
ATAGGCTTCCGAAACTGAAGCAGTTATACAAACAGATTTTATCTGACAGAATCTCCCTGA
GCTTTCTGCCGGATGCTTTCACTGATGGGAAGCAGGTTCTGAAAGCGATTTTCGATTTTT
ATAAGATTAACTTACTGAGCTACACGATTGAAGGTCAAGAAGAATCTCAAAACTTACTGC
TCTTGATCCGTCAAACCATTGAAAATCTATCATCGTTCGATACGCAGAAAATCTACCTCA
AAAACGATACTCACCTGACTACGATCTCTCAGCAGGTTTTCGGGGATTTTAGTGTATTTT
CAACAGCTCTGAACTACTGGTATGAAACCAAAGTCAATCCGAAATTCGAGACGGAATATT
CTAAGGCCAACGAAAAAAAACGTGAGATTCTTGATAAAGCTAAAGCCGTATTTACTAAAC
AGGATTACTTTTCTATTGCTTTCCTGCAGGAAGTTTTATCGGAGTATATCCTGACCCTGG
ATCATACATCTGATATCGTTAAAAAACACAGCAGCAATTGCATCGCTGACTATTTCAAAA
ACCACTTTGTCGCCAAAAAAGAAAACGAAACAGACAAGACTTTCGATTTCATTGCTAACA
TCACCGCAAAATACCAGTGTATTCAGGGTATCTTGGAAAACGCCGACCAATACGAAGACG
AACTGAAACAAGATCAGAAGCTGATCGATAATTTAAAATTCTTCTTAGATGCAATCCTGG
AGCTGCTGCACTTCATCAAACCGCTTCATTTAAAGAGCGAGTCCATTACCGAAAAGGACA
CCGCCTTCTATGACGTTTTTGAAAATTATTATGAAGCCCTCTCCTTGCTGACTCCGCTGT
ATAATATGGTACGCAATTACGTAACCCAGAAACCATATTCTACCGAAAAAATTAAACTGA
ACTTTGAAAACGCACAGCTGCTCAACGGTTGGGACGCGAATAAAGAAGGTGACTACCTCA
CCACCATCCTGAAAAAAGATGGTAACTATTTTCTGGCAATTATGGATAAGAAACATAATA
AAGCATTCCAGAAATTTCCTGAAGGGAAAGAAAATTACGAAAAGATGGTGTACAAACTCT
TACCTGGAGTTAACAAAATGTTGCCGAAAGTATTTTTTAGTAATAAGAACATCGCGTACT
TTAACCCGTCCAAAGAACTGCTGGAAAATTATAAAAAGGAGACGCATAAGAAAGGGGATA
CCTTTAACCTGGAACATTGCCATACCTTAATAGACTTCTTCAAGGATTCCCTGAATAAAC
ACGAGGATTGGAAATATTTCGATTTTCAGTTTAGTGAGACCAAGTCATACCAGGATCTTA
GCGGCTTTTATCGCGAAGTAGAACACCAAGGCTATAAAATTAACTTCAAAAACATCGACA
GCGAATACATCGACGGTTTAGTTAACGAGGGCAAACTGTTTCTGTTCCAGATCTATTCAA
AGGATTTTAGCCCGTTCTCTAAAGGCAAACCAAATATGCATACGTTGTACTGGAAAGCAC
TGTTTGAAGAGCAAAACCTGCAGAATGTGATTTATAAACTGAACGGCCAAGCTGAGATTT
TTTTCCGTAAAGCCTCGATTAAACCGAAAAATATCATCCTTCATAAGAAGAAAATAAAGA
TCGCTAAAAAACACTTCATAGATAAAAAAACCAAAACCTCCGAAATAGTGCCTGTTCAAA
CAATTAAGAACTTGAATATGTACTACCAGGGCAAGATATCGGAAAAGGAGTTGACTCAAG
ACGATCTTCGCTATATCGATAACTTTTCGATTTTTAACGAAAAAAACAAGACGATCGACA
TCATCAAAGATAAACGCTTCACTGTAGATAAGTTCCAGTTTCATGTGCCGATTACTATGA
ACTTCAAAGCTACCGGGGGTAGCTATATCAACCAAACGGTGTTGGAATACCTGCAGAATA
ACCCGGAAGTCAAAATCATTGGGCTGGACCGCGGAGAACGTCACCTTGTGTACTTGACCT
TAATCGATCAGCAAGGCAACATCTTAAAACAAGAATCGCTGAATACCATTACGGATTCAA
AGATTAGCACCCCGTATCATAAGCTGCTCGATAACAAGGAGAATGAGCGCGACCTGGCCC
GTAAAAACTGGGGCACGGTGGAAAACATTAAGGAGTTAAAGGAGGGTTATATTTCCCAGG
TAGTGCATAAGATCGCCACTCTCATGCTCGAGGAAAATGCGATCGTTGTCATGGAAGACT
TAAACTTCGGATTTAAACGTGGGCGATTTAAAGTAGAGAAACAAATCTACCAGAAGTTAG
AAAAAATGCTGATTGACAAATTAAATTACTTGGTCCTAAAAGACAAACAGCCGCAAGAAT
TGGGTGGATTATACAACGCCCTCCAACTTACCAATAAATTCGAAAGTTTTCAGAAAATGG
GTAAACAGTCAGGCTTTCTTTTTTATGTTCCTGCGTGGAACACATCCAAAATCGACCCTA
CAACCGGCTTCGTCAATTACTTCTATACTAAATATGAAAACGTCGACAAAGCAAAAGCAT
TCTTTGAAAAGTTCGAAGCAATACGTTTTAACGCTGAGAAAAAATATTTCGAGTTCGAAG
TCAAGAAATACTCAGACTTTAACCCCAAAGCTGAGGGCACACAGCAAGCGTGGACAATCT
GCACCTACGGCGAGCGCATCGAAACGAAGCGTCAAAAAGATCAGAATAACAAATTTGTTT
CAACACCTATCAACCTGACCGAGAAGATTGAAGACTTCTTAGGTAAAAATCAGATTGTTT
ATGGCGACGGTAACTGTATAAAATCTCAAATAGCCTCAAAGGATGATAAAGCATTTTTCG
AAACATTATTATATTGGTTCAAAATGACACTGCAGATGCGCAATAGTGAGACGCGTACAG
ATATTGATTATCTTATCAGCCCGGTCATGAACGACAACGGTACTTTTTACAACTCCAGAG
ACTATGAAAAACTTGAGAATCCAACTCTCCCCAAAGATGCTGATGCGAACGGTGCTTATC
ACATCGCGAAAAAAGGTCTGATGCTGCTGAACAAAATCGACCAAGCCGATCTGACTAAGA
AAGTTGACCTAAGCATTTCAAATCGGGACTGGTTACAGTTTGTTCAAAAGAACAAATGAG
AAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATGTAGGGAGACCCTCAGG
TTAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACA
SEQ AAAATTCcatGCAAAATGCTCCGGTTTCATGTCATCAAAATGATGACGTAATTAAGCATT
ID GATAATTGAGATCCCTCTCCCTGACAGGATGATTACATAAATAATAGTGACAAAAATAAA
NO: TTATTTATTTATCCAGAAAATGAATTGGAAAATCAGGAGAGCGTTTTCAATCCTACCTCT
69 GGCGCAGTTGATATGTcaaaCAGGTtgccgtcactgcgtcttttactggctcttctcgct
aaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccat
gacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattattt
gcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctac
ctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagcac
cgcctatctcgtgtgagataggcggagatacgaactttaagAAGGAGatataccATGGAA
CAGGAATATTATCTGGGCTTGGACATGGGCACCGGTTCCGTCGGCTGGGCTGTTACTGAC
AGTGAATATCACGTTCTAAGAAAGCATGGTAAGGCATTGTGGGGTGTAAGACTTTTCGAA
TCTGCTTCCACTGCTGAAGAGCGTAGAATGTTTAGAACGAGTCGACGTAGGCTAGACAGG
CGCAATTGGAGAATCGAAATTTTACAAGAAATTTTTGCGGAAGAGATATCTAAGAAAGAC
CCAGGCTTTTTCCTGAGAATGAAGGAATCTAAGTATTACCCTGAGGATAAAAGAGATATA
AATGGTAACTGTCCCGAATTGCCTTACGCATTATTTGTGGACGATGATTTTACCGATAAG
GATTACCATAAAAAGTTCCCAACTATCTACCATTTACGCAAAATGTTAATGAATACAGAG
GAAACCCCAGACATAAGACTAGTTTATCTGGCAATACACCATATGATGAAACATAGAGGC
CATTTCTTACTTTCCGGGGATATCAACGAAATCAAAGAGTTTGGTACCACATTTAGTAAG
TTACTGGAAAACATAAAGAATGAAGAATTGGATTGGAACTTAGAACTCGGAAAAGAAGAA
TACGCGGTTGTCGAATCTATCCTGAAGGATAATATGCTGAATAGGTCGACCAAAAAAACT
AGGCTGATCAAAGCACTGAAAGCCAAATCTATCTGCGAAAAAGCTGTTTTAAATTTACTT
GCTGGTGGCACTGTTAAGTTATCAGACATTTTTGGTTTGGAAGAATTGAACGAAACCGAG
CGTCCAAAAATTAGTTTCGCTGATAATGGCTACGATGATTACATTGGTGAGGTGGAAAAC
GAGTTGGGCGAACAATTTTATATTATAGAGACAGCTAAGGCAGTCTATGACTGGGCTGTT
TTAGTAGAAATCCTTGGTAAATACACATCTATCTCCGAAGCGAAAGTTGCTACTTACGAA
AAGCACAAGTCCGATCTCCAGTTTTTGAAGAAAATTGTCAGGAAATATCTGACTAAGGAA
GAATATAAAGATATTTTCGTTAGTACCTCTGACAAACTGAAAAATTACTCCGCTTACATC
GGGATGACCAAGATTAATGGCAAAAAAGTTGATCTGCAAAGCAAAAGGTGTTCGAAGGAA
GAATTTTATGATTTCATTAAAAAGAATGTCTTAAAAAAATTAGAAGGTCAGCCAGAATAC
GAATATTTGAAAGAAGAACTGGAAAGAGAGACATTCTTACCAAAACAAGTCAACAGAGAT
AATGGGGTAATTCCATATCAAATTCACCTCTACGAATTAAAAAAAATTTTAGGCAATTTA
CGCGATAAAATTGACCTTATCAAAGAAAATGAGGATAAGCTGGTTCAACTCTTTGAATTC
AGAATACCCTATTATGTGGGCCCACTGAACAAGATTGATGACGGCAAAGAAGGTAAATTC
ACATGGGCCGTCCGCAAATCCAATGAAAAAATTTACCCATGGAACTTTGAAAATGTAGTA
GATATTGAAGCGTCTGCGGAGAAATTTATTCGAAGAATGACTAATAAATGCACTTACTTG
ATGGGAGAGGATGTTCTGCCTAAAGACAGCTTATTATACAGCAAGTACATGGTTCTAAAC
GAACTTAACAACGTTAAGTTGGACGGTGAGAAATTAAGTGTAGAATTGAAACAAAGATTG
TATACTGACGTCTTCTGCAAGTACAGAAAAGTGACAGTTAAAAAAATTAAGAATTACTTG
AAGTGCGAAGGTATAATTTCTGGAAACGTAGAGATTACTGGTATTGATGGTGATTTCAAA
GCATCCCTAACAGCTTACCACGATTTCAAGGAAATCCTGACAGGAACTGAACTCGCAAAA
AAAGATAAAGAAAACATTATTACTAATATTGTTCTTTTCGGTGATGACAAGAAATTGTTG
AAGAAAAGACTGAATAGACTTTACCCCCAGATTACTCCCAATCAACTTAAGAAAATTTGT
GCTTTGTCTTACACAGGATGGGGTCGTTTTTCAAAAAAGTTCTTAGAAGAGATTACCGCA
CCTGATCCAGAAACAGGCGAAGTATGGAATATAATTACCGCCTTATGGGAATCGAACAAT
AATCTTATGCAACTTCTGAGCAATGAATATCGTTTCATGGAAGAAGTTGAGACTTACAAC
ATGGGCAAACAGACGAAGACTTTATCCTATGAAACTGTGGAAAATATGTATGTATCACCT
TCTGTCAAGAGACAAATTTGGCAAACCTTAAAAATTGTCAAAGAATTAGAAAAGGTAATG
AAGGAGTCTCCTAAACGTGTGTTTATTGAAATGGCTAGAGAAAAACAAGAGTCAAAAAGA
ACCGAGTCAAGAAAGAAGCAGTTAATCGATTTATATAAGGCTTGTAAAAACGAAGAGAAA
GATTGGGTTAAAGAATTGGGGGACCAAGAGGAACAAAAACTACGGTCGGATAAGTTGTAT
TTATACTATACGCAAAAGGGACGATGTATGTATTCCGGCGAGGTAATAGAATTGAAGGAT
TTATGGGACAATACAAAATATGACATAGACCATATATATCCCCAATCAAAAACGATGGAC
GATAGCTTGAACAATAGAGTACTCGTGAAAAAAAAATATAATGCGACCAAATCTGATAAG
TATCCTCTGAATGAAAATATCAGACATGAAAGAAAGGGGTTCTGGAAGTCCTTGTTAGAT
GGTGGGTTTATAAGCAAAGAAAAGTACGAGCGTCTAATAAGAAACACGGAGTTATCGCCA
GAAGAACTCGCTGGTTTTATTGAGAGGCAAATCGTGGAAACGAGACAATCTACCAAAGCC
GTTGCTGAGATCCTAAAGCAAGTTTTCCCAGAGTCGGAGATTGTCTATGTCAAAGCTGGC
ACAGTGAGCAGGTTTAGGAAAGACTTCGAACTATTAAAGGTAAGAGAAGTGAACGATTTA
CATCACGCAAAGGACGCTTACCTAAATATCGTTGTAGGTAACTCATATTATGTTAAATTT
ACCAAGAACGCCTCTTGGTTTATAAAGGAGAACCCAGGTAGAACATATAACCTGAAAAAG
ATGTTCACCTCTGGTTGGAATATTGAGAGAAACGGAGAAGTCGCATGGGAAGTTGGTAAG
AAAGGGACTATAGTGACAGTAAAGCAAATTATGAACAAAAATAATATCCTCGTTACAAGG
CAGGTTCATGAAGCAAAGGGCGGCCTTTTTGACCAACAAATTATGAAGAAAGGGAAAGGT
CAAATTGCAATAAAAGAAACCGATGAGAGACTAGCGTCAATAGAAAAGTATGGTGGCTAT
AATAAAGCTGCGGGTGCATACTTTATGCTTGTTGAATCAAAAGACAAGAAAGGTAAGACT
ATTAGAACTATAGAATTTATACCCCTGTACCTTAAAAACAAAATTGAATCGGATGAGTCA
ATCGCGTTAAATTTTCTAGAGAAAGGAAGGGGTTTAAAAGAACCAAAGATCCTGTTAAAA
AAGATTAAGATTGACACCTTGTTCGATGTAGATGGATTTAAAATGTGGTTATCTGGCAGA
ACAGGCGATAGACTTTTGTTTAAGTGCGCTAATCAATTAATTTTGGATGAGAAAATCATT
GTCACAATGAAAAAAATAGTTAAGTTTATTCAGAGAAGACAAGAAAACAGGGAGTTGAAA
TTATCTGATAAAGATGGTATCGACAATGAAGTTTTAATGGAAATCTACAATACATTCGTT
GATAAACTTGAAAATACCGTATATCGAATCAGGTTAAGTGAACAAGCCAAAACATTAATT
GATAAACAAAAAGAATTTGAAAGGCTATCACTGGAAGACAAATCCTCCACCCTATTTGAA
ATTTTGCATATATTCCAGTGCCAATCTTCAGCAGCTAATTTAAAAATGATTGGCGGACCT
GGGAAAGCCGGCATCCTAGTGATGAACAATAATATCTCCAAGTGTAACAAAATATCAATT
ATTAACCAATCTCCGACAGGTATTTTTGAAAATGAAATAGACTTGCTTAAGATATAAGAA
ATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATTTATTATATCGCGTTGATT
ATTGATGCTGTTTTTAGTTTTAACGGCAATTAATATATGTGTTATTAATTGAATGAATTT
TATCATTCATAATAAGTATGTGTAGGATCAAGCTCAGGTTAAATATTCACTCAGGAAGTT
ATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATAACAAGTGTTAAGGGATGTTA
TTTCC
SEQ AATCAGGAGAGCGTTTTCAATCCTACCTCTGGCGCAGTTGATATGTCAAACAGGTTGCCG
ID TCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAG
NO: CATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAA
70 TCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC
ATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGT
TTCTCCATACCCGTTTTTTTGGGCTAGCAGTAATACGACTCACTATAGGGGTCTCATCTC
GTGTGAGATAGGCGGAGATACGAACTTTAAGAGGAGGATATACCATGCACCATCATCATC
ACCATTCTTTCGACTCTTTCACCAACCTGTACTCTCTGTCTAAAACCCTGAAATTCGAAA
TGCGTCCGGTTGGTAACACCCAGAAAATGCTGGACAACGCGGGTGTTTTCGAAAAAGACA
AACTGATCCAGAAAAAATACGGTAAAACCAAACCGTACTTCGACCGTCTGCACCGTGAAT
TCATCGAAGAAGCGCTGACCGGTGTTGAACTGATCGGTCTGGACGAAAACTTCCGTACCC
TGGTTGACTGGCAGAAAGACAAAAAAAACAACGTTGCGATGAAAGCGTACGAAAACTCTC
TGCAGCGTCTGCGTACCGAAATCGGTAAAATCTTCAACCTGAAAGCGGAAGACTGGGTTA
AAAACAAATACCCGATCCTGGGTCTGAAAAACAAAAACACCGACATCCTGTTCGAAGAAG
CGGTTTTCGGTATCCTGAAAGCGCGTTACGGTGAAGAAAAAGACACCTTCATCGAAGTTG
AAGAAATCGACAAAACCGGTAAATCTAAAATCAACCAGATCTCTATCTTCGACTCTTGGA
AAGGTTTCACCGGTTACTTCAAAAAATTCTTCGAAACCCGTAAAAACTTCTACAAAAACG
ACGGTACCTCTACCGCGATCGCGACCCGTATCATCGACCAGAACCTGAAACGTTTCATCG
ACAACCTGTCTATCGTTGAATCTGTTCGTCAGAAAGTTGACCTGGCGGAAACCGAAAAAT
CTTTCTCTATCTCTCTGTCTCAGTTCTTCTCTATCGACTTCTACAACAAATGCCTGCTGC
AGGACGGTATCGACTACTACAACAAAATCATCGGTGGTGAAACCCTGAAAAACGGTGAAA
AACTGATCGGTCTGAACGAACTGATCAACCAGTACCGTCAGAACAACAAAGACCAGAAAA
TCCCGTTCTTCAAACTGCTGGACAAACAGATCCTGTCTGAAAAAATCCTGTTCCTGGACG
AAATCAAAAACGACACCGAACTGATCGAAGCGCTGTCTCAGTTCGCGAAAACCGCGGAAG
AAAAAACCAAAATCGTTAAAAAACTGTTCGCGGACTTCGTTGAAAACAACTCTAAATACG
ACCTGGCGCAGATCTACATCTCTCAGGAAGCGTTCAACACCATCTCTAACAAATGGACCT
CTGAAACCGAAACCTTCGCGAAATACCTGTTCGAAGCGATGAAATCTGGTAAACTGGCGA
AATACGAAAAAAAAGACAACTCTTACAAATTCCCGGACTTCATCGCGCTGTCTCAGATGA
AATCTGCGCTGCTGTCTATCTCTCTGGAAGGTCACTTCTGGAAAGAAAAATACTACAAAA
TCTCTAAATTCCAGGAAAAAACCAACTGGGAACAGTTCCTGGCGATCTTCCTGTACGAAT
TCAACTCTCTGTTCTCTGACAAAATCAACACCAAAGACGGTGAAACCAAACAGGTTGGTT
ACTACCTGTTCGCGAAAGACCTGCACAACCTGATCCTGTCTGAACAGATCGACATCCCGA
AAGACTCTAAAGTTACCATCAAAGACTTCGCGGACTCTGTTCTGACCATCTACCAGATGG
CGAAATACTTCGCGGTTGAAAAAAAACGTGCGTGGCTGGCGGAATACGAACTGGACTCTT
TCTACACCCAGCCGGACACCGGTTACCTGCAGTTCTACGACAACGCGTACGAAGACATCG
TTCAGGTTTACAACAAACTGCGTAACTACCTGACCAAAAAACCGTACTCTGAAGAAAAAT
GGAAACTGAACTTCGAAAACTCTACCCTGGCGAACGGTTGGGACAAAAACAAAGAATCTG
ACAACTCTGCGGTTATCCTGCAGAAAGGTGGTAAATACTACCTGGGTCTGATCACCAAAG
GTCACAACAAAATCTTCGACGACCGTTTCCAGGAAAAATTCATCGTTGGTATCGAAGGTG
GTAAATACGAAAAAATCGTTTACAAATTCTTCCCGGACCAGGCGAAAATGTTCCCGAAAG
TTTGCTTCTCTGCGAAAGGTCTGGAATTCTTCCGTCCGTCTGAAGAAATCCTGCGTATCT
ACAACAACGCGGAATTCAAAAAAGGTGAAACCTACTCTATCGACTCTATGCAGAAACTGA
TCGACTTCTACAAAGACTGCCTGACCAAATACGAAGGTTGGGCGTGCTACACCTTCCGTC
ACCTGAAACCGACCGAAGAATACCAGAACAACATCGGTGAATTCTTCCGTGACGTTGCGG
AAGACGGTTACCGTATCGACTTCCAGGGTATCTCTGACCAGTACATCCACGAAAAAAACG
AAAAAGGTGAACTGCACCTGTTCGAAATCCACAACAAAGACTGGAACCTGGACAAAGCGC
GTGACGGTAAATCTAAAACCACCCAGAAAAACCTGCACACCCTGTACTTCGAATCTCTGT
TCTCTAACGACAACGTTGTTCAGAACTTCCCGATCAAACTGAACGGTCAGGCGGAAATCT
TCTACCGTCCGAAAACCGAAAAAGACAAACTGGAATCTAAAAAAGACAAAAAAGGTAACA
AAGTTATCGACCACAAACGTTACTCTGAAAACAAAATCTTCTTCCACGTTCCGCTGACCC
TGAACCGTACCAAAAACGACTCTTACCGTTTCAACGCGCAGATCAACAACTTCCTGGCGA
ACAACAAAGACATCAACATCATCGGTGTTGACCGTGGTGAAAAACACCTGGTTTACTACT
CTGTTATCACCCAGGCGTCTGACATCCTGGAATCTGGTTCTCTGAACGAACTGAACGGTG
TTAACTACGCGGAAAAACTGGGTAAAAAAGCGGAAAACCGTGAACAGGCGCGTCGTGACT
GGCAGGACGTTCAGGGTATCAAAGACCTGAAAAAAGGTTACATCTCTCAGGTTGTTCGTA
AACTGGCGGACCTGGCGATCAAACACAACGCGATCATCATCCTGGAAGACCTGAACATGC
GTTTCAAACAGGTTCGTGGTGGTATCGAAAAATCTATCTACCAGCAGCTGGAAAAAGCGC
TGATCGACAAACTGTCTTTCCTGGTTGACAAAGGTGAAAAAAACCCGGAACAGGCGGGTC
ACCTGCTGAAAGCGTACCAGCTGTCTGCGCCGTTCGAAACCTTCCAGAAAATGGGTAAAC
AGACCGGTATCATCTTCTACACCCAGGCGTCTTACACCTCTAAATCTGACCCGGTTACCG
GTTGGCGTCCGCACCTGTACCTGAAATACTTCTCTGCGAAAAAAGCGAAAGACGACATCG
CGAAATTCACCAAAATCGAATTCGTTAACGACCGTTTCGAACTGACCTACGACATCAAAG
ACTTCCAGCAGGCGAAAGAATACCCGAACAAAACCGTTTGGAAAGTTTGCTCTAACGTTG
AACGTTTCCGTTGGGACAAAAACCTGAACCAGAACAAAGGTGGTTACACCCACTACACCA
ACATCACCGAAAACATCCAGGAACTGTTCACCAAATACGGTATCGACATCACCAAAGACC
TGCTGACCCAGATCTCTACCATCGACGAAAAACAGAACACCTCTTTCTTCCGTGACTTCA
TCTTCTACTTCAACCTGATCTGCCAGATCCGTAACACCGACGACTCTGAAATCGCGAAAA
AAAACGGTAAAGACGACTTCATCCTGTCTCCGGTTGAACCGTTCTTCGACTCTCGTAAAG
ACAACGGTAACAAACTGCCGGAAAACGGTGACGACAACGGTGCGTACAACATCGCGCGTA
AAGGTATCGTTATCCTGAACAAAATCTCTCAGTACTCTGAAAAAAACGAAAACTGCGAAA
AAATGAAATGGGGTGACCTGTACGTTTCTAACATCGACTGGGACAACTTCGTTGAAATCA
TCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATGTAGGGAGACCCTCAGGTTAAAT
ATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACA
SEQ AATCAGGAGAGCGTTTTCAATCCTACCTCTGGCGCAGTTGATATGTCAAACAGGTTGCCG
ID TCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAG
NO: CATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAA
71 TCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC
ATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGT
TTCTCCATACCCGTTTTTTTGGGCTAGCAGTAATACGACTCACTATAGGGGTCTCATCTC
GTGTGAGATAGGCGGAGATACGAACTTTAAGAGGAGGATATACCATGCACCATCATCATC
ACCATAACAAATTCGAAAACTTCACCGGTCTGTACCCGATCTCTAAAACCCTGCGTTTCG
AACTGATCCCGCAGGGTAAAACCCTGGAATACATCGAAAAATCTGAAATCCTGGAAAACG
ACAACTACCGTGCGGAAAAATACGAAGAAGTTAAAGACATCATCGACGGTTACCACAAAT
GGTTCATCAACGAAACCCTGCACGACCTGCACATCAACTGGTCTGAACTGAAAGTTGCGC
TGGAAAACAACCGTATCGAAAAATCTGACGCGTCTAAAAAAGAACTGCAGCGTGTTCAGA
AAATCAAACGTGAAGAAATCTACAACGCGTTCATCGAACACGAAGCGTTCCAGTACCTGT
TCAAAGAAAACCTGCTGTCTGACCTGCTGCCGATCCAGATCGAACAGTCTGAAGACCTGG
ACGCGGAAAAAAAAAAACAGGCGGTTGAAACCTTCAACCGTTTCTCTACCTACTTCACCG
GTTTCCACGAAAACCGTAAAAACATCTACTCTAAAGAAGGTATCTCTACCTCTGTTACCT
ACCGTATCGTTCACGACAACTTCCCGAAATTCCTGGAAAACATGAAAGTTTTCGAAATCC
TGCGTAACGAATGCCCGGAAGTTATCTCTGACACCGCGAACGAACTGGCGCCGTTCATCG
ACGGTGTTCGTATCGAAGACATCTTCCTGATCGACTTCTTCAACTCTACCTTCTCTCAGA
ACGGTATCGACTACTACAACCGTATCCTGGGTGGTGTTACCACCGAAACCGGTGAAAAAT
ACCGTGGTATCAACGAATTCACCAACCTGTACCGTCAGCAGCACCCGGAATTCGGTAAAT
CTAAAAAAGCGACCAAAATGGTTGTTCTGTTCAAACAGATCCTGTCTGACCGTGACACCC
TGTCTTTCATCCCGGAAATGTTCGGTAACGACAAACAGGTTCAGAACTCTATCCAGCTGT
TCTACAACCGTGAAATCTCTCAGTTCGAAAACGAAGGTGTTAAAACCGACGTTTGCACCG
CGCTGGCGACCCTGACCTCTAAAATCGCGGAATTCGACACCGAAAAAATCTACATCCAGC
AGCCGGAACTGCCGAACGTTTCTCAGCGTCTGTTCGGTTCTTGGAACGAACTGAACGCGT
GCCTGTTCAAATACGCGGAACTGAAATTCGGTACCGCGGAAAAAGTTGCGAACCGTAAAA
AAATCGACAAATGGCTGAAATCTGACCTGTTCTCTTTCACCGAACTGAACAAAGCGCTGG
AATTCTCTGGTAAAGACGAACGTATCGAAAACTACTTCTCTGAAACCGGTATCTTCGCGC
AGCTGGTTAAAACCGGTTTCGACGAAGCGCAGTCTATCCTGGAAACCGAATACACCTCTG
AAGTTCACCTGAAAGACCAGCAGACCGACATCGAAAAAATCAAAACCTTCCTGGACGCGC
TGCAGAACCTGATGCACCTGCTGAAATCTCTGTGCGTTTCTGAAGAAGCGGACCGTGACG
CGGCGTTCTACAACGAATTCGACATGCTGTACAACCAGCTGAAACTGGTTGTTCCGCTGT
ACAACAAAGTTCGTAACTACATCACCCAGAAACTGTTCCGTTCTGACAAAATCAAAATCT
ACTTCGAAAACAAAGGTCAGTTCCTGGGTGGTTGGGTTGACTCTCAGACCGAAAACTCTG
ACAACGGTACCCAGGCGGGTGGTTACATCTTCCGTAAAGAAAACGTTATCAACGAATACG
ACTACTACCTGGGTATCTGCTCTGACCCGAAACTGTTCCGTCGTACCACCATCGTTTCTG
AAAACGACCGTTCTTCTTTCGAACGTCTGGACTACTACCAGCTGAAAACCGCGTCTGTTT
ACGGTAACTCTTACTGCGGTAAACACCCGTACACCGAAGACAAAAACGAACTGGTTAACT
CTATCGACCGTTTCGTTCACCTGTCTGGTAACAACATCCTGATCGAAAAAATCGCGAAAG
ACAAAGTTAAATCTAACCCGACCACCAACACCCCGTCTGGTTACCTGAACTTCATCCACC
GTGAAGCGCCGAACACCTACGAATGCCTGCTGCAGGACGAAAACTTCGTTTCTCTGAACC
AGCGTGTTGTTTCTGCGCTGAAAGCGACCCTGGCGACCCTGGTTCGTGTTCCGAAAGCGC
TGGTTTACGCGAAAAAAGACTACCACCTGTTCTCTGAAATCATCAACGACATCGACGAAC
TGTCTTACGAAAAAGCGTTCTCTTACTTCCCGGTTTCTCAGACCGAATTCGAAAACTCTT
CTAACCGTACCATCAAACCGCTGCTGCTGTTCAAAATCTCTAACAAAGACCTGTCTTTCG
CGGAAAACTTCGAAAAAGGTAACCGTCAGAAAATCGGTAAAAAAAACCTGCACACCCTGT
ACTTCGAAGCGCTGATGAAAGGTAACCAGGACACCATCGACATCGGTACCGGTATGGTTT
TCCACCGTGTTAAATCTCTGAACTACAACGAAAAAACCCTGAAATACGGTCACCACTCTA
CCCAGCTGAACGAAAAATTCTCTTACCCGATCATCAAAGACAAACGTTTCGCGTCTGACA
AATTCCTGTTCCACCTGTCTACCGAAATCAACTACAAAGAAAAACGTAAACCGCTGAACA
ACTCTATCATCGAATTCCTGACCAACAACCCGGACATCAACATCATCGGTCTGGACCGTG
GTGAACGTCACCTGATCTACCTGACCCTGATCAACCAGAAAGGTGAAATCCTGCGTCAGA
AAACCTTCAACATCGTTGGTAACACCAACTACCACGAAAAACTGAACCAGCGTGAAAAAG
AACGTGACAACGCGCGTAAATCTTGGGCGACCATCGGTAAAATCAAAGAACTGAAAGAAG
GTTTCCTGTCTCTGGTTATCCACGAAATCGCGAAAATCATGGTTGAAAACAACGCGATCG
TTGTTCTGGAAGACCTGAACTTCGGTTTCAAACGTGGTCGTTTCAAAGTTGAAAAACAGA
TCTACCAGAAATTCGAAAAAATGCTGATCGACAAACTGAACTACCTGGTTTTCAAAGACA
AAAAAGCGAACGAAGCGGGTGGTGTTCTGAAAGGTTACCAGCTGGCGGAAAAATTCGAAT
CTTTCCAGAAAATGGGTAAACAGTCTGGTTTCCTGTTCTACGTTCCGGCGGCGTACACCT
CTAAAATCGACCCGACCACCGGTTTCGTTAACATGCTGAACCTGAACTACACCAACATGA
AAGACGCGCAGACCCTGCTGTCTGGTATGGACAAAATCTCTTTCAACGCGGACGCGAACT
ACTTCGAATTCGAACTGGACTACGAAAAATTCAAAACCAACCAGACCGACCACACCAACA
AATGGACCATCTGCACCGTTGGTGAAAAACGTTTCACCTACAACTCTGCGACCAAAGAAA
CCACCACCGTTAACGTTACCGAAGACCTGAAAAAACTGCTGGACAAATTCGAAGTTAAAT
ACTCTAACGGTGACAACATCAAAGACGAAATCTGCCGTCAGACCGACGCGAAATTCTTCG
AAATCATCCTGTGGCTGCTGAAACTGACCATGCAGATGCGTAACTCTAACACCAAAACCG
AAGAAGACTTCATCCTGTCTCCGGTTAAAAACTCTAACGGTGAATTCTTCCGTTCTAACG
ACGACGCGAACGGTATCTGGCCGGCGGACGCGGACGCGAACGGTGCGTACCACATCGCGC
TGAAAGGTCTGTACCTGGTTAAAGAATGCTTCAACAAAAACGAAAAATCTCTGAAAATCG
AACACAAAAACTGGTTCAAATTCGCGCAGACCCGTTTCAACGGTTCTCTGACCAAAAACG
GTTAAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATGTAGGGAGACC
CTCAGGTTAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACA
SEQ AATCAGGAGAGCGTTTTCAATCCTACCTCTGGCGCAGTTGATATGTCAAACAGGTTGCCG
ID TCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAG
NO: CATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAA
72 TCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC
ATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGT
TTCTCCATACCCGTTTTTTTGGGCTAGCAGTAATACGACTCACTATAGGGGTCTCATCTC
GTGTGAGATAGGCGGAGATACGAACTTTAAGAGGAGGATATACCATGCACCATCATCATC
ACCATACCCAGTTCGAAGGTTTCACCAACCTGTACCAGGTTTCTAAAACCCTGCGTTTCG
AACTGATCCCGCAGGGTAAAACCCTGAAACACATCCAGGAACAGGGTTTCATCGAAGAAG
ACAAAGCGCGTAACGACCACTACAAAGAACTGAAACCGATCATCGACCGTATCTACAAAA
CCTACGCGGACCAGTGCCTGCAGCTGGTTCAGCTGGACTGGGAAAACCTGTCTGCGGCGA
TCGACTCTTACCGTAAAGAAAAAACCGAAGAAACCCGTAACGCGCTGATCGAAGAACAGG
CGACCTACCGTAACGCGATCCACGACTACTTCATCGGTCGTACCGACAACCTGACCGACG
CGATCAACAAACGTCACGCGGAAATCTACAAAGGTCTGTTCAAAGCGGAACTGTTCAACG
GTAAAGTTCTGAAACAGCTGGGTACCGTTACCACCACCGAACACGAAAACGCGCTGCTGC
GTTCTTTCGACAAATTCACCACCTACTTCTCTGGTTTCTACGAAAACCGTAAAAACGTTT
TCTCTGCGGAAGACATCTCTACCGCGATCCCGCACCGTATCGTTCAGGACAACTTCCCGA
AATTCAAAGAAAACTGCCACATCTTCACCCGTCTGATCACCGCGGTTCCGTCTCTGCGTG
AACACTTCGAAAACGTTAAAAAAGCGATCGGTATCTTCGTTTCTACCTCTATCGAAGAAG
TTTTCTCTTTCCCGTTCTACAACCAGCTGCTGACCCAGACCCAGATCGACCTGTACAACC
AGCTGCTGGGTGGTATCTCTCGTGAAGCGGGTACCGAAAAAATCAAAGGTCTGAACGAAG
TTCTGAACCTGGCGATCCAGAAAAACGACGAAACCGCGCACATCATCGCGTCTCTGCCGC
ACCGTTTCATCCCGCTGTTCAAACAGATCCTGTCTGACCGTAACACCCTGTCTTTCATCC
TGGAAGAATTCAAATCTGACGAAGAAGTTATCCAGTCTTTCTGCAAATACAAAACCCTGC
TGCGTAACGAAAACGTTCTGGAAACCGCGGAAGCGCTGTTCAACGAACTGAACTCTATCG
ACCTGACCCACATCTTCATCTCTCACAAAAAACTGGAAACCATCTCTTCTGCGCTGTGCG
ACCACTGGGACACCCTGCGTAACGCGCTGTACGAACGTCGTATCTCTGAACTGACCGGTA
AAATCACCAAATCTGCGAAAGAAAAAGTTCAGCGTTCTCTGAAACACGAAGACATCAACC
TGCAGGAAATCATCTCTGCGGCGGGTAAAGAACTGTCTGAAGCGTTCAAACAGAAAACCT
CTGAAATCCTGTCTCACGCGCACGCGGCGCTGGACCAGCCGCTGCCGACCACCCTGAAAA
AACAGGAAGAAAAAGAAATCCTGAAATCTCAGCTGGACTCTCTGCTGGGTCTGTACCACC
TGCTGGACTGGTTCGCGGTTGACGAATCTAACGAAGTTGACCCGGAATTCTCTGCGCGTC
TGACCGGTATCAAACTGGAAATGGAACCGTCTCTGTCTTTCTACAACAAAGCGCGTAACT
ACGCGACCAAAAAACCGTACTCTGTTGAAAAATTCAAACTGAACTTCCAGATGCCGACCC
TGGCGTCTGGTTGGGACGTTAACAAAGAAAAAAACAACGGTGCGATCCTGTTCGTTAAAA
ACGGTCTGTACTACCTGGGTATCATGCCGAAACAGAAAGGTCGTTACAAAGCGCTGTCTT
TCGAACCGACCGAAAAAACCTCTGAAGGTTTCGACAAAATGTACTACGACTACTTCCCGG
ACGCGGCGAAAATGATCCCGAAATGCTCTACCCAGCTGAAAGCGGTTACCGCGCACTTCC
AGACCCACACCACCCCGATCCTGCTGTCTAACAACTTCATCGAACCGCTGGAAATCACCA
AAGAAATCTACGACCTGAACAACCCGGAAAAAGAACCGAAAAAATTCCAGACCGCGTACG
CGAAAAAAACCGGTGACCAGAAAGGTTACCGTGAAGCGCTGTGCAAATGGATCGACTTCA
CCCGTGACTTCCTGTCTAAATACACCAAAACCACCTCTATCGACCTGTCTTCTCTGCGTC
CGTCTTCTCAGTACAAAGACCTGGGTGAATACTACGCGGAACTGAACCCGCTGCTGTACC
ACATCTCTTTCCAGCGTATCGCGGAAAAAGAAATCATGGACGCGGTTGAAACCGGTAAAC
TGTACCTGTTCCAGATCTACAACAAAGACTTCGCGAAAGGTCACCACGGTAAACCGAACC
TGCACACCCTGTACTGGACCGGTCTGTTCTCTCCGGAAAACCTGGCGAAAACCTCTATCA
AACTGAACGGTCAGGCGGAACTGTTCTACCGTCCGAAATCTCGTATGAAACGTATGGCGC
ACCGTCTGGGTGAAAAAATGCTGAACAAAAAACTGAAAGACCAGAAAACCCCGATCCCGG
ACACCCTGTACCAGGAACTGTACGACTACGTTAACCACCGTCTGTCTCACGACCTGTCTG
ACGAAGCGCGTGCGCTGCTGCCGAACGTTATCACCAAAGAAGTTTCTCACGAAATCATCA
AAGACCGTCGTTTCACCTCTGACAAATTCTTCTTCCACGTTCCGATCACCCTGAACTACC
AGGCGGCGAACTCTCCGTCTAAATTCAACCAGCGTGTTAACGCGTACCTGAAAGAACACC
CGGAAACCCCGATCATCGGTATCGACCGTGGTGAACGTAACCTGATCTACATCACCGTTA
TCGACTCTACCGGTAAAATCCTGGAACAGCGTTCTCTGAACACCATCCAGCAGTTCGACT
ACCAGAAAAAACTGGACAACCGTGAAAAAGAACGTGTTGCGGCGCGTCAGGCGTGGTCTG
TTGTTGGTACCATCAAAGACCTGAAACAGGGTTACCTGTCTCAGGTTATCCACGAAATCG
TTGACCTGATGATCCACTACCAGGCGGTTGTTGTTCTGGAAAACCTGAACTTCGGTTTCA
AATCTAAACGTACCGGTATCGCGGAAAAAGCGGTTTACCAGCAGTTCGAAAAAATGCTGA
TCGACAAACTGAACTGCCTGGTTCTGAAAGACTACCCGGCGGAAAAAGTTGGTGGTGTTC
TGAACCCGTACCAGCTGACCGACCAGTTCACCTCTTTCGCGAAAATGGGTACCCAGTCTG
GTTTCCTGTTCTACGTTCCGGCGCCGTACACCTCTAAAATCGACCCGCTGACCGGTTTCG
TTGACCCGTTCGTTTGGAAAACCATCAAAAACCACGAATCTCGTAAACACTTCCTGGAAG
GTTTCGACTTCCTGCACTACGACGTTAAAACCGGTGACTTCATCCTGCACTTCAAAATGA
ACCGTAACCTGTCTTTCCAGCGTGGTCTGCCGGGTTTCATGCCGGCGTGGGACATCGTTT
TCGAAAAAAACGAAACCCAGTTCGACGCGAAAGGTACCCCGTTCATCGCGGGTAAACGTA
TCGTTCCGGTTATCGAAAACCACCGTTTCACCGGTCGTTACCGTGACCTGTACCCGGCGA
ACGAACTGATCGCGCTGCTGGAAGAAAAAGGTATCGTTTTCCGTGACGGTTCTAACATCC
TGCCGAAACTGCTGGAAAACGACGACTCTCACGCGATCGACACCATGGTTGCGCTGATCC
GTTCTGTTCTGCAGATGCGTAACTCTAACGCGGCGACCGGTGAAGACTACATCAACTCTC
CGGTTCGTGACCTGAACGGTGTTTGCTTCGACTCTCGTTTCCAGAACCCGGAATGGCCGA
TGGACGCGGACGCGAACGGTGCGTACCACATCGCGCTGAAAGGTCAGCTGCTGCTGAACC
ACCTGAAAGAATCTAAAGACCTGAAACTGCAGAACGGTATCTCTAACCAGGACTGGCTGG
CGTACATCCAGGAACTGCGTAACTAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTT
ATCTGAAATGTAGGGAGACCCTCAGGTTAAATATTCACTCAGGAAGTTATTACTCAGGAA
GCAAAGAGGATTACA
SEQ AATCAGGAGAGCGTTTTCAATCCTACCTCTGGCGCAGTTGATATGTCAAACAGGTTGCCG
ID TCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAG
NO: CATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAA
73 TCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC
ATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGT
TTCTCCATACCCGTTTTTTTGGGCTAGCAGTAATACGACTCACTATAGGGGTCTCATCTC
GTGTGAGATAGGCGGAGATACGAACTTTAAGAGGAGGATATACCATGCACCATCATCATC
ACCATGCGGTTAAATCTATCAAAGTTAAACTGCGTCTGGACGACATGCCGGAAATCCGTG
CGGGTCTGTGGAAACTGCACAAAGAAGTTAACGCGGGTGTTCGTTACTACACCGAATGGC
TGTCTCTGCTGCGTCAGGAAAACCTGTACCGTCGTTCTCCGAACGGTGACGGTGAACAGG
AATGCGACAAAACCGCGGAAGAATGCAAAGCGGAACTGCTGGAACGTCTGCGTGCGCGTC
AGGTTGAAAACGGTCACCGTGGTCCGGCGGGTTCTGACGACGAACTGCTGCAGCTGGCGC
GTCAGCTGTACGAACTGCTGGTTCCGCAGGCGATCGGTGCGAAAGGTGACGCGCAGCAGA
TCGCGCGTAAATTCCTGTCTCCGCTGGCGGACAAAGACGCGGTTGGTGGTCTGGGTATCG
CGAAAGCGGGTAACAAACCGCGTTGGGTTCGTATGCGTGAAGCGGGTGAACCGGGTTGGG
AAGAAGAAAAAGAAAAAGCGGAAACCCGTAAATCTGCGGACCGTACCGCGGACGTTCTGC
GTGCGCTGGCGGACTTCGGTCTGAAACCGCTGATGCGTGTTTACACCGACTCTGAAATGT
CTTCTGTTGAATGGAAACCGCTGCGTAAAGGTCAGGCGGTTCGTACCTGGGACCGTGACA
TGTTCCAGCAGGCGATCGAACGTATGATGTCTTGGGAATCTTGGAACCAGCGTGTTGGTC
AGGAATACGCGAAACTGGTTGAACAGAAAAACCGTTTCGAACAGAAAAACTTCGTTGGTC
AGGAACACCTGGTTCACCTGGTTAACCAGCTGCAGCAGGACATGAAAGAAGCGTCTCCGG
GTCTGGAATCTAAAGAACAGACCGCGCACTACGTTACCGGTCGTGCGCTGCGTGGTTCTG
ACAAAGTTTTCGAAAAATGGGGTAAACTGGCGCCGGACGCGCCGTTCGACCTGTACGACG
CGGAAATCAAAAACGTTCAGCGTCGTAACACCCGTCGTTTCGGTTCTCACGACCTGTTCG
CGAAACTGGCGGAACCGGAATACCAGGCGCTGTGGCGTGAAGACGCGTCTTTCCTGACCC
GTTACGCGGTTTACAACTCTATCCTGCGTAAACTGAACCACGCGAAAATGTTCGCGACCT
TCACCCTGCCGGACGCGACCGCGCACCCGATCTGGACCCGTTTCGACAAACTGGGTGGTA
ACCTGCACCAGTACACCTTCCTGTTCAACGAATTCGGTGAACGTCGTCACGCGATCCGTT
TCCACAAACTGCTGAAAGTTGAAAACGGTGTTGCGCGTGAAGTTGACGACGTTACCGTTC
CGATCTCTATGTCTGAACAGCTGGACAACCTGCTGCCGCGTGACCCGAACGAACCGATCG
CGCTGTACTTCCGTGACTACGGTGCGGAACAGCACTTCACCGGTGAATTCGGTGGTGCGA
AAATCCAGTGCCGTCGTGACCAGCTGGCGCACATGCACCGTCGTCGTGGTGCGCGTGACG
TTTACCTGAACGTTTCTGTTCGTGTTCAGTCTCAGTCTGAAGCGCGTGGTGAACGTCGTC
CGCCGTACGCGGCGGTTTTCCGTCTGGTTGGTGACAACCACCGTGCGTTCGTTCACTTCG
ACAAACTGTCTGACTACCTGGCGGAACACCCGGACGACGGTAAACTGGGTTCTGAAGGTC
TGCTGTCTGGTCTGCGTGTTATGTCTGTTGACCTGGGTCTGCGTACCTCTGCGTCTATCT
CTGTTTTCCGTGTTGCGCGTAAAGACGAACTGAAACCGAACTCTAAAGGTCGTGTTCCGT
TCTTCTTCCCGATCAAAGGTAACGACAACCTGGTTGCGGTTCACGAACGTTCTCAGCTGC
TGAAACTGCCGGGTGAAACCGAATCTAAAGACCTGCGTGCGATCCGTGAAGAACGTCAGC
GTACCCTGCGTCAGCTGCGTACCCAGCTGGCGTACCTGCGTCTGCTGGTTCGTTGCGGTT
CTGAAGACGTTGGTCGTCGTGAACGTTCTTGGGCGAAACTGATCGAACAGCCGGTTGACG
CGGCGAACCACATGACCCCGGACTGGCGTGAAGCGTTCGAAAACGAACTGCAGAAACTGA
AATCTCTGCACGGTATCTGCTCTGACAAAGAATGGATGGACGCGGTTTACGAATCTGTTC
GTCGTGTTTGGCGTCACATGGGTAAACAGGTTCGTGACTGGCGTAAAGACGTTCGTTCTG
GTGAACGTCCGAAAATCCGTGGTTACGCGAAAGACGTTGTTGGTGGTAACTCTATCGAAC
AGATCGAATACCTGGAACGTCAGTACAAATTCCTGAAATCTTGGTCTTTCTTCGGTAAAG
TTTCTGGTCAGGTTATCCGTGCGGAAAAAGGTTCTCGTTTCGCGATCACCCTGCGTGAAC
ACATCGACCACGCGAAAGAAGACCGTCTGAAAAAACTGGCGGACCGTATCATCATGGAAG
CGCTGGGTTACGTTTACGCGCTGGACGAACGTGGTAAAGGTAAATGGGTTGCGAAATACC
CGCCGTGCCAGCTGATCCTGCTGGAAGAACTGTCTGAATACCAGTTCAACAACGACCGTC
CGCCGTCTGAAAACAACCAGCTGATGCAGTGGTCTCACCGTGGTGTTTTCCAGGAACTGA
TCAACCAGGCGCAGGTTCACGACCTGCTGGTTGGTACCATGTACGCGGCGTTCTCTTCTC
GTTTCGACGCGCGTACCGGTGCGCCGGGTATCCGTTGCCGTCGTGTTCCGGCGCGTTGCA
CCCAGGAACACAACCCGGAACCGTTCCCGTGGTGGCTGAACAAATTCGTTGTTGAACACA
CCCTGGACGCGTGCCCGCTGCGTGCGGACGACCTGATCCCGACCGGTGAAGGTGAAATCT
TCGTTTCTCCGTTCTCTGCGGAAGAAGGTGACTTCCACCAGATCCACGCGGACCTGAACG
CGGCGCAGAACCTGCAGCAGCGTCTGTGGTCTGACTTCGACATCTCTCAGATCCGTCTGC
GTTGCGACTGGGGTGAAGTTGACGGTGAACTGGTTCTGATCCCGCGTCTGACCGGTAAAC
GTACCGCGGACTCTTACTCTAACAAAGTTTTCTACACCAACACCGGTGTTACCTACTACG
AACGTGAACGTGGTAAAAAACGTCGTAAAGTTTTCGCGCAGGAAAAACTGTCTGAAGAAG
AAGCGGAACTGCTGGTTGAAGCGGACGAAGCGCGTGAAAAATCTGTTGTTCTGATGCGTG
ACCCGTCTGGTATCATCAACCGTGGTAACTGGACCCGTCAGAAAGAATTCTGGTCTATGG
TTAACCAGCGTATCGAAGGTTACCTGGTTAAACAGATCCGTTCTCGTGTTCCGCTGCAGG
ACTCTGCGTGCGAAAACACCGGTGACATCTAAGAAATCATCCTTAGCGAAAGCTAAGGAT
TTTTTTTATCTGAAATGTAGGGAGACCCTCAGGTTAAATATTCACTCAGGAAGTTATTAC
TCAGGAAGCAAAGAGGATTACA
SEQ AATCAGGAGAGCGTTTTCAATCCTACCTCTGGCGCAGTTGATATGTCAAACAGGTTGCCG
ID TCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAG
NO: CATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAA
74 TCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC
ATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGT
TTCTCCATACCCGTTTTTTTGGGCTAGCAGTAATACGACTCACTATAGGGGTCTCATCTC
GTGTGAGATAGGCGGAGATACGAACTTTAAGAGGAGGATATACCATGCACCATCATCATC
ACCATGCGACCCGTTCTTTCATCCTGAAAATCGAACCGAACGAAGAAGTTAAAAAAGGTC
TGTGGAAAACCCACGAAGTTCTGAACCACGGTATCGCGTACTACATGAACATCCTGAAAC
TGATCCGTCAGGAAGCGATCTACGAACACCACGAACAGGACCCGAAAAACCCGAAAAAAG
TTTCTAAAGCGGAAATCCAGGCGGAACTGTGGGACTTCGTTCTGAAAATGCAGAAATGCA
ACTCTTTCACCCACGAAGTTGACAAAGACGTTGTTTTCAACATCCTGCGTGAACTGTACG
AAGAACTGGTTCCGTCTTCTGTTGAAAAAAAAGGTGAAGCGAACCAGCTGTCTAACAAAT
TCCTGTACCCGCTGGTTGACCCGAACTCTCAGTCTGGTAAAGGTACCGCGTCTTCTGGTC
GTAAACCGCGTTGGTACAACCTGAAAATCGCGGGTGACCCGTCTTGGGAAGAAGAAAAAA
AAAAATGGGAAGAAGACAAAAAAAAAGACCCGCTGGCGAAAATCCTGGGTAAACTGGCGG
AATACGGTCTGATCCCGCTGTTCATCCCGTTCACCGACTCTAACGAACCGATCGTTAAAG
AAATCAAATGGATGGAAAAATCTCGTAACCAGTCTGTTCGTCGTCTGGACAAAGACATGT
TCATCCAGGCGCTGGAACGTTTCCTGTCTTGGGAATCTTGGAACCTGAAAGTTAAAGAAG
AATACGAAAAAGTTGAAAAAGAACACAAAACCCTGGAAGAACGTATCAAAGAAGACATCC
AGGCGTTCAAATCTCTGGAACAGTACGAAAAAGAACGTCAGGAACAGCTGCTGCGTGACA
CCCTGAACACCAACGAATACCGTCTGTCTAAACGTGGTCTGCGTGGTTGGCGTGAAATCA
TCCAGAAATGGCTGAAAATGGACGAAAACGAACCGTCTGAAAAATACCTGGAAGTTTTCA
AAGACTACCAGCGTAAACACCCGCGTGAAGCGGGTGACTACTCTGTTTACGAATTCCTGT
CTAAAAAAGAAAACCACTTCATCTGGCGTAACCACCCGGAATACCCGTACCTGTACGCGA
CCTTCTGCGAAATCGACAAAAAAAAAAAAGACGCGAAACAGCAGGCGACCTTCACCCTGG
CGGACCCGATCAACCACCCGCTGTGGGTTCGTTTCGAAGAACGTTCTGGTTCTAACCTGA
ACAAATACCGTATCCTGACCGAACAGCTGCACACCGAAAAACTGAAAAAAAAACTGACCG
TTCAGCTGGACCGTCTGATCTACCCGACCGAATCTGGTGGTTGGGAAGAAAAAGGTAAAG
TTGACATCGTTCTGCTGCCGTCTCGTCAGTTCTACAACCAGATCTTCCTGGACATCGAAG
AAAAAGGTAAACACGCGTTCACCTACAAAGACGAATCTATCAAATTCCCGCTGAAAGGTA
CCCTGGGTGGTGCGCGTGTTCAGTTCGACCGTGACCACCTGCGTCGTTACCCGCACAAAG
TTGAATCTGGTAACGTTGGTCGTATCTACTTCAACATGACCGTTAACATCGAACCGACCG
AATCTCCGGTTTCTAAATCTCTGAAAATCCACCGTGACGACTTCCCGAAATTCGTTAACT
TCAAACCGAAAGAACTGACCGAATGGATCAAAGACTCTAAAGGTAAAAAACTGAAATCTG
GTATCGAATCTCTGGAAATCGGTCTGCGTGTTATGTCTATCGACCTGGGTCAGCGTCAGG
CGGCGGCGGCGTCTATCTTCGAAGTTGTTGACCAGAAACCGGACATCGAAGGTAAACTGT
TCTTCCCGATCAAAGGTACCGAACTGTACGCGGTTCACCGTGCGTCTTTCAACATCAAAC
TGCCGGGTGAAACCCTGGTTAAATCTCGTGAAGTTCTGCGTAAAGCGCGTGAAGACAACC
TGAAACTGATGAACCAGAAACTGAACTTCCTGCGTAACGTTCTGCACTTCCAGCAGTTCG
AAGACATCACCGAACGTGAAAAACGTGTTACCAAATGGATCTCTCGTCAGGAAAACTCTG
ACGTTCCGCTGGTTTACCAGGACGAACTGATCCAGATCCGTGAACTGATGTACAAACCGT
ACAAAGACTGGGTTGCGTTCCTGAAACAGCTGCACAAACGTCTGGAAGTTGAAATCGGTA
AAGAAGTTAAACACTGGCGTAAATCTCTGTCTGACGGTCGTAAAGGTCTGTACGGTATCT
CTCTGAAAAACATCGACGAAATCGACCGTACCCGTAAATTCCTGCTGCGTTGGTCTCTGC
GTCCGACCGAACCGGGTGAAGTTCGTCGTCTGGAACCGGGTCAGCGTTTCGCGATCGACC
AGCTGAACCACCTGAACGCGCTGAAAGAAGACCGTCTGAAAAAAATGGCGAACACCATCA
TCATGCACGCGCTGGGTTACTGCTACGACGTTCGTAAAAAAAAATGGCAGGCGAAAAACC
CGGCGTGCCAGATCATCCTGTTCGAAGACCTGTCTAACTACAACCCGTACGAAGAACGTT
CTCGTTTCGAAAACTCTAAACTGATGAAATGGTCTCGTCGTGAAATCCCGCGTCAGGTTG
CGCTGCAGGGTGAAATCTACGGTCTGCAGGTTGGTGAAGTTGGTGCGCAGTTCTCTTCTC
GTTTCCACGCGAAAACCGGTTCTCCGGGTATCCGTTGCTCTGTTGTTACCAAAGAAAAAC
TGCAGGACAACCGTTTCTTCAAAAACCTGCAGCGTGAAGGTCGTCTGACCCTGGACAAAA
TCGCGGTTCTGAAAGAAGGTGACCTGTACCCGGACAAAGGTGGTGAAAAATTCATCTCTC
TGTCTAAAGACCGTAAACTGGTTACCACCCACGCGGACATCAACGCGGCGCAGAACCTGC
AGAAACGTTTCTGGACCCGTACCCACGGTTTCTACAAAGTTTACTGCAAAGCGTACCAGG
TTGACGGTCAGACCGTTTACATCCCGGAATCTAAAGACCAGAAACAGAAAATCATCGAAG
AATTCGGTGAAGGTTACTTCATCCTGAAAGACGGTGTTTACGAATGGGGTAACGCGGGTA
AACTGAAAATCAAAAAAGGTTCTTCTAAACAGTCTTCTTCTGAACTGGTTGACTCTGACA
TCCTGAAAGACTCTTTCGACCTGGCGTCTGAACTGAAAGGTGAAAAACTGATGCTGTACC
GTGACCCGTCTGGTAACGTTTTCCCGTCTGACAAATGGATGGCGGCGGGTGTTTTCTTCG
GTAAACTGGAACGTATCCTGATCTCTAAACTGACCAACCAGTACTCTATCTCTACCATCG
AAGACGACTCTTCTAAACAGTCTATGTAAGAAATCATCCTTAGCGAAAGCTAAGGATTTT
TTTTATCTGAAATGTAGGGAGACCCTCAGGTTAAATATTCACTCAGGAAGTTATTACTCA
GGAAGCAAAGAGGATTACA
SEQ AATCAGGAGAGCGTTTTCAATCCTACCTCTGGCGCAGTTGATATGTCAAACAGGTTGCCG
ID TCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAG
NO: CATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAA
75 TCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC
ATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGT
TTCTCCATACCCGTTTTTTTGGGCTAGCAGTAATACGACTCACTATAGGGGTCTCATCTC
GTGTGAGATAGGCGGAGATACGAACTTTAAGAGGAGGATATACCATGCACCATCATCATC
ACCATCCGACCCGTACCATCAACCTGAAACTGGTTCTGGGTAAAAACCCGGAAAACGCGA
CCCTGCGTCGTGCGCTGTTCTCTACCCACCGTCTGGTTAACCAGGCGACCAAACGTATCG
AAGAATTCCTGCTGCTGTGCCGTGGTGAAGCGTACCGTACCGTTGACAACGAAGGTAAAG
AAGCGGAAATCCCGCGTCACGCGGTTCAGGAAGAAGCGCTGGCGTTCGCGAAAGCGGCGC
AGCGTCACAACGGTTGCATCTCTACCTACGAAGACCAGGAAATCCTGGACGTTCTGCGTC
AGCTGTACGAACGTCTGGTTCCGTCTGTTAACGAAAACAACGAAGCGGGTGACGCGCAGG
CGGCGAACGCGTGGGTTTCTCCGCTGATGTCTGCGGAATCTGAAGGTGGTCTGTCTGTTT
ACGACAAAGTTCTGGACCCGCCGCCGGTTTGGATGAAACTGAAAGAAGAAAAAGCGCCGG
GTTGGGAAGCGGCGTCTCAGATCTGGATCCAGTCTGACGAAGGTCAGTCTCTGCTGAACA
AACCGGGTTCTCCGCCGCGTTGGATCCGTAAACTGCGTTCTGGTCAGCCGTGGCAGGACG
ACTTCGTTTCTGACCAGAAAAAAAAACAGGACGAACTGACCAAAGGTAACGCGCCGCTGA
TCAAACAGCTGAAAGAAATGGGTCTGCTGCCGCTGGTTAACCCGTTCTTCCGTCACCTGC
TGGACCCGGAAGGTAAAGGTGTTTCTCCGTGGGACCGTCTGGCGGTTCGTGCGGCGGTTG
CGCACTTCATCTCTTGGGAATCTTGGAACCACCGTACCCGTGCGGAATACAACTCTCTGA
AACTGCGTCGTGACGAATTCGAAGCGGCGTCTGACGAATTCAAAGACGACTTCACCCTGC
TGCGTCAGTACGAAGCGAAACGTCACTCTACCCTGAAATCTATCGCGCTGGCGGACGACT
CTAACCCGTACCGTATCGGTGTTCGTTCTCTGCGTGCGTGGAACCGTGTTCGTGAAGAAT
GGATCGACAAAGGTGCGACCGAAGAACAGCGTGTTACCATCCTGTCTAAACTGCAGACCC
AGCTGCGTGGTAAATTCGGTGACCCGGACCTGTTCAACTGGCTGGCGCAGGACCGTCACG
TTCACCTGTGGTCTCCGCGTGACTCTGTTACCCCGCTGGTTCGTATCAACGCGGTTGACA
AAGTTCTGCGTCGTCGTAAACCGTACGCGCTGATGACCTTCGCGCACCCGCGTTTCCACC
CGCGTTGGATCCTGTACGAAGCGCCGGGTGGTTCTAACCTGCGTCAGTACGCGCTGGACT
GCACCGAAAACGCGCTGCACATCACCCTGCCGCTGCTGGTTGACGACGCGCACGGTACCT
GGATCGAAAAAAAAATCCGTGTTCCGCTGGCGCCGTCTGGTCAGATCCAGGACCTGACCC
TGGAAAAACTGGAAAAAAAAAAAAACCGTCTGTACTACCGTTCTGGTTTCCAGCAGTTCG
CGGGTCTGGCGGGTGGTGCGGAAGTTCTGTTCCACCGTCCGTACATGGAACACGACGAAC
GTTCTGAAGAATCTCTGCTGGAACGTCCGGGTGCGGTTTGGTTCAAACTGACCCTGGACG
TTGCGACCCAGGCGCCGCCGAACTGGCTGGACGGTAAAGGTCGTGTTCGTACCCCGCCGG
AAGTTCACCACTTCAAAACCGCGCTGTCTAACAAATCTAAACACACCCGTACCCTGCAGC
CGGGTCTGCGTGTTCTGTCTGTTGACCTGGGTATGCGTACCTTCGCGTCTTGCTCTGTTT
TCGAACTGATCGAAGGTAAACCGGAAACCGGTCGTGCGTTCCCGGTTGCGGACGAACGTT
CTATGGACTCTCCGAACAAACTGTGGGCGAAACACGAACGTTCTTTCAAACTGACCCTGC
CGGGTGAAACCCCGTCTCGTAAAGAAGAAGAAGAACGTTCTATCGCGCGTGCGGAAATCT
ACGCGCTGAAACGTGACATCCAGCGTCTGAAATCTCTGCTGCGTCTGGGTGAAGAAGACA
ACGACAACCGTCGTGACGCGCTGCTGGAACAGTTCTTCAAAGGTTGGGGTGAAGAAGACG
TTGTTCCGGGTCAGGCGTTCCCGCGTTCTCTGTTCCAGGGTCTGGGTGCGGCGCCGTTCC
GTTCTACCCCGGAACTGTGGCGTCAGCACTGCCAGACCTACTACGACAAAGCGGAAGCGT
GCCTGGCGAAACACATCTCTGACTGGCGTAAACGTACCCGTCCGCGTCCGACCTCTCGTG
AAATGTGGTACAAAACCCGTTCTTACCACGGTGGTAAATCTATCTGGATGCTGGAATACC
TGGACGCGGTTCGTAAACTGCTGCTGTCTTGGTCTCTGCGTGGTCGTACCTACGGTGCGA
TCAACCGTCAGGACACCGCGCGTTTCGGTTCTCTGGCGTCTCGTCTGCTGCACCACATCA
ACTCTCTGAAAGAAGACCGTATCAAAACCGGTGCGGACTCTATCGTTCAGGCGGCGCGTG
GTT
ACATCCCGCTGCCGCACGGTAAAGGTTGGGAACAGCGTTACGAACCGTGCCAGCTGATCC
TGTTCGAAGACCTGGCGCGTTACCGTTTCCGTGTTGACCGTCCGCGTCGTGAAAACTCTC
AGCTGATGCAGTGGAACCACCGTGCGATCGTTGCGGAAACCACCATGCAGGCGGAACTGT
ACGGTCAGATCGTTGAAAACACCGCGGCGGGTTTCTCTTCTCGTTTCCACGCGGCGACCG
GTGCGCCGGGTGTTCGTTGCCGTTTCCTGCTGGAACGTGACTTCGACAACGACCTGCCGA
AACCGTACCTGCTGCGTGAACTGTCTTGGATGCTGGGTAACACCAAAGTTGAATCTGAAG
AAGAAAAACTGCGTCTGCTGTCTGAAAAAATCCGTCCGGGTTCTCTGGTTCCGTGGGACG
GTGGTGAACAGTTCGCGACCCTGCACCCGAAACGTCAGACCCTGTGCGTTATCCACGCGG
ACATGAACGCGGCGCAGAACCTGCAGCGTCGTTTCTTCGGTCGTTGCGGTGAAGCGTTCC
GTCTGGTTTGCCAGCCGCACGGTGACGACGTTCTGCGTCTGGCGTCTACCCCGGGTGCGC
GTCTGCTGGGTGCGCTGCAGCAGCTGGAAAACGGTCAGGGTGCGTTCGAACTGGTTCGTG
ACATGGGTTCTACCTCTCAGATGAACCGTTTCGTTATGAAATCTCTGGGTAAAAAAAAAA
TCAAACCGCTGCAGGACAACAACGGTGACGACGAACTGGAAGACGTTCTGTCTGTTCTGC
CGGAAGAAGACGACACCGGTCGTATCACCGTTTTCCGTGACTCTTCTGGTATCTTCTTCC
CGTGCAACGTTTGGATCCCGGCGAAACAGTTCTGGCCGGCGGTTCGTGCGATGATCTGGA
AAGTTATGGCGTCTCACTCTCTGGGTTAAGAAATCATCCTTAGCGAAAGCTAAGGATTTT
TTTTATCTGAAATGTAGGGAGACCCTCAGGTTAAATATTCACTCAGGAAGTTATTACTCA
GGAAGCAAAGAGGATTACA
SEQ AATCAGGAGAGCGTTTTCAATCCTACCTCTGGCGCAGTTGATATGTCAAACAGGTTGCCG
ID TCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAG
NO: CATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAA
76 TCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC
ATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGT
TTCTCCATACCCGTTTTTTTGGGCTAGCAGTAATACGACTCACTATAGGGGTCTCATCTC
GTGTGAGATAGGCGGAGATACGAACTTTAAGAGGAGGATATACCATGCACCATCATCATC
ACCATACCAAACTGCGTCACCGTCAGAAAAAACTGACCCACGACTGGGCGGGTTCTAAAA
AACGTGAAGTTCTGGGTTCTAACGGTAAACTGCAGAACCCGCTGCTGATGCCGGTTAAAA
AAGGTCAGGTTACCGAATTCCGTAAAGCGTTCTCTGCGTACGCGCGTGCGACCAAAGGTG
AAATGACCGACGGTCGTAAAAACATGTTCACCCACTCTTTCGAACCGTTCAAAACCAAAC
CGTCTCTGCACCAGTGCGAACTGGCGGACAAAGCGTACCAGTCTCTGCACTCTTACCTGC
CGGGTTCTCTGGCGCACTTCCTGCTGTCTGCGCACGCGCTGGGTTTCCGTATCTTCTCTA
AATCTGGTGAAGCGACCGCGTTCCAGGCGTCTTCTAAAATCGAAGCGTACGAATCTAAAC
TGGCGTCTGAACTGGCGTGCGTTGACCTGTCTATCCAGAACCTGACCATCTCTACCCTGT
TCAACGCGCTGACCACCTCTGTTCGTGGTAAAGGTGAAGAAACCTCTGCGGACCCGCTGA
TCGCGCGTTTCTACACCCTGCTGACCGGTAAACCGCTGTCTCGTGACACCCAGGGTCCGG
AACGTGACCTGGCGGAAGTTATCTCTCGTAAAATCGCGTCTTCTTTCGGTACCTGGAAAG
AAATGACCGCGAACCCGCTGCAGTCTCTGCAGTTCTTCGAAGAAGAACTGCACGCGCTGG
ACGCGAACGTTTCTCTGTCTCCGGCGTTCGACGTTCTGATCAAAATGAACGACCTGCAGG
GTGACCTGAAAAACCGTACCATCGTTTTCGACCCGGACGCGCCGGTTTTCGAATACAACG
CGGAAGACCCGGCGGACATCATCATCAAACTGACCGCGCGTTACGCGAAAGAAGCGGTTA
TCAAAAACCAGAACGTTGGTAACTACGTTAAAAACGCGATCACCACCACCAACGCGAACG
GTCTGGGTTGGCTGCTGAACAAAGGTCTGTCTCTGCTGCCGGTTTCTACCGACGACGAAC
TGCTGGAATTCATCGGTGTTGAACGTTCTCACCCGTCTTGCCACGCGCTGATCGAACTGA
TCGCGCAGCTGGAAGCGCCGGAACTGTTCGAAAAAAACGTTTTCTCTGACACCCGTTCTG
AAGTTCAGGGTATGATCGACTCTGCGGTTTCTAACCACATCGCGCGTCTGTCTTCTTCTC
GTAACTCTCTGTCTATGGACTCTGAAGAACTGGAACGTCTGATCAAATCTTTCCAGATCC
ACACCCCGCACTGCTCTCTGTTCATCGGTGCGCAGTCTCTGTCTCAGCAGCTGGAATCTC
TGCCGGAAGCGCTGCAGTCTGGTGTTAACTCTGCGGACATCCTGCTGGGTTCTACCCAGT
ACATGCTGACCAACTCTCTGGTTGAAGAATCTATCGCGACCTACCAGCGTACCCTGAACC
GTATCAACTACCTGTCTGGTGTTGCGGGTCAGATCAACGGTGCGATCAAACGTAAAGCGA
TCGACGGTGAAAAAATCCACCTGCCGGCGGCGTGGTCTGAACTGATCTCTCTGCCGTTCA
TCGGTCAGCCGGTTATCGACGTTGAATCTGACCTGGCGCACCTGAAAAACCAGTACCAGA
CCCTGTCTAACGAATTCGACACCCTGATCTCTGCGCTGCAGAAAAACTTCGACCTGAACT
TCAACAAAGCGCTGCTGAACCGTACCCAGCACTTCGAAGCGATGTGCCGTTCTACCAAAA
AAAACGCGCTGTCTAAACCGGAAATCGTTTCTTACCGTGACCTGCTGGCGCGTCTGACCT
CTTGCCTGTACCGTGGTTCTCTGGTTCTGCGTCGTGCGGGTATCGAAGTTCTGAAAAAAC
ACAAAATCTTCGAATCTAACTCTGAACTGCGTGAACACGTTCACGAACGTAAACACTTCG
TTTTCGTTTCTCCGCTGGACCGTAAAGCGAAAAAACTGCTGCGTCTGACCGACTCTCGTC
CGGACCTGCTGCACGTTATCGACGAAATCCTGCAGCACGACAACCTGGAAAACAAAGACC
GTGAATCTCTGTGGCTGGTTCGTTCTGGTTACCTGCTGGCGGGTCTGCCGGACCAGCTGT
CTTCTTCTTTCATCAACCTGCCGATCATCACCCAGAAAGGTGACCGTCGTCTGATCGACC
TGATCCAGTACGACCAGATCAACCGTGACGCGTTCGTTATGCTGGTTACCTCTGCGTTCA
AATCTAACCTGTCTGGTCTGCAGTACCGTGCGAACAAACAGTCTTTCGTTGTTACCCGTA
CCCTGTCTCCGTACCTGGGTTCTAAACTGGTTTACGTTCCGAAAGACAAAGACTGGCTGG
TTCCGTCTCAGATGTTCGAAGGTCGTTTCGCGGACATCCTGCAGTCTGACTACATGGTTT
GGAAAGACGCGGGTCGTCTGTGCGTTATCGACACCGCGAAACACCTGTCTAACATCAAAA
AATCTGTTTTCTCTTCTGAAGAAGTTCTGGCGTTCCTGCGTGAACTGCCGCACCGTACCT
TCATCCAGACCGAAGTTCGTGGTCTGGGTGTTAACGTTGACGGTATCGCGTTCAACAACG
GTGACATCCCGTCTCTGAAAACCTTCTCTAACTGCGTTCAGGTTAAAGTTTCTCGTACCA
ACACCTCTCTGGTTCAGACCCTGAACCGTTGGTTCGAAGGTGGTAAAGTTTCTCCGCCGT
CTATCCAGTTCGAACGTGCGTACTACAAAAAAGACGACCAGATCCACGAAGACGCGGCGA
AACGTAAAATCCGTTTCCAGATGCCGGCGACCGAACTGGTTCACGCGTCTGACGACGCGG
GTTGGACCCCGTCTTACCTGCTGGGTATCGACCCGGGTGAATACGGTATGGGTCTGTCTC
TGGTTTCTATCAACAACGGTGAAGTTCTGGACTCTGGTTTCATCCACATCAACTCTCTGA
TCAACTTCGCGTCTAAAAAATCTAACCACCAGACCAAAGTTGTTCCGCGTCAGCAGTACA
AATCTCCGTACGCGAACTACCTGGAACAGTCTAAAGACTCTGCGGCGGGTGACATCGCGC
ACATCCTGGACCGTCTGATCTACAAACTGAACGCGCTGCCGGTTTTCGAAGCGCTGTCTG
GTAACTCTCAGTCTGCGGCGGACCAGGTTTGGACCAAAGTTCTGTCTTTCTACACCTGGG
GTGACAACGACGCGCAGAACTCTATCCGTAAACAGCACTGGTTCGGTGCGTCTCACTGGG
ACATCAAAGGTATGCTGCGTCAGCCGCCGACCGAAAAAAAACCGAAACCGTACATCGCGT
TCCCGGGTTCTCAGGTTTCTTCTTACGGTAACTCTCAGCGTTGCTCTTGCTGCGGTCGTA
ACCCGATCGAACAGCTGCGTGAAATGGCGAAAGACACCTCTATCAAAGAACTGAAAATCC
GTAACTCTGAAATCCAGCTGTTCGACGGTACCATCAAACTGTTCAACCCGGACCCGTCTA
CCGTTATCGAACGTCGTCGTCACAACCTGGGTCCGTCTCGTATCCCGGTTGCGGACCGTA
CCTTCAAAAACATCTCTCCGTCTTCTCTGGAATTCAAAGAACTGATCACCATCGTTTCTC
GTTCTATCCGTCACTCTCCGGAATTCATCGCGAAAAAACGTGGTATCGGTTCTGAATACT
TCTGCGCGTACTCTGACTGCAACTCTTCTCTGAACTCTGAAGCGAACGCGGCGGCGAACG
TTGCGCAGAAATTCCAGAAACAGCTGTTCTTCGAACTGTAAGAAATCATCCTTAGCGAAA
GCTAAGGATTTTTTTTATCTGAAATGTAGGGAGACCCTCAGGTTAAATATTCACTCAGGA
AGTTATTACTCAGGAAGCAAAGAGGATTACA
SEQ AATCAGGAGAGCGTTTTCAATCCTACCTCTGGCGCAGTTGATATGTCAAACAGGTTGCCG
ID TCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAG
NO: CATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAA
77 TCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC
ATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGT
TTCTCCATACCCGTTTTTTTGGGCTAGCAGTAATACGACTCACTATAGGGGTCTCATCTC
GTGTGAGATAGGCGGAGATACGAACTTTAAGAGGAGGATATACCATGCACCATCATCATC
ACCATAAACGTATCCTGAACTCTCTGAAAGTTGCGGCGCTGCGTCTGCTGTTCCGTGGTA
AAGGTTCTGAACTGGTTAAAACCGTTAAATACCCGCTGGTTTCTCCGGTTCAGGGTGCGG
TTGAAGAACTGGCGGAAGCGATCCGTCACGACAACCTGCACCTGTTCGGTCAGAAAGAAA
TCGTTGACCTGATGGAAAAAGACGAAGGTACCCAGGTTTACTCTGTTGTTGACTTCTGGC
TGGACACCCTGCGTCTGGGTATGTTCTTCTCTCCGTCTGCGAACGCGCTGAAAATCACCC
TGGGTAAATTCAACTCTGACCAGGTTTCTCCGTTCCGTAAAGTTCTGGAACAGTCTCCGT
TCTTCCTGGCGGGTCGTCTGAAAGTTGAACCGGCGGAACGTATCCTGTCTGTTGAAATCC
GTAAAATCGGTAAACGTGAAAACCGTGTTGAAAACTACGCGGCGGACGTTGAAACCTGCT
TCATCGGTCAGCTGTCTTCTGACGAAAAACAGTCTATCCAGAAACTGGCGAACGACATCT
GGGACTCTAAAGACCACGAAGAACAGCGTATGCTGAAAGCGGACTTCTTCGCGATCCCGC
TGATCAAAGACCCGAAAGCGGTTACCGAAGAAGACCCGGAAAACGAAACCGCGGGTAAAC
AGAAACCGCTGGAACTGTGCGTTTGCCTGGTTCCGGAACTGTACACCCGTGGTTTCGGTT
CTATCGCGGACTTCCTGGTTCAGCGTCTGACCCTGCTGCGTGACAAAATGTCTACCGACA
CCGCGGAAGACTGCCTGGAATACGTTGGTATCGAAGAAGAAAAAGGTAACGGTATGAACT
CTCTGCTGGGTACCTTCCTGAAAAACCTGCAGGGTGACGGTTTCGAACAGATCTTCCAGT
TCATGCTGGGTTCTTACGTTGGTTGGCAGGGTAAAGAAGACGTTCTGCGTGAACGTCTGG
ACCTGCTGGCGGAAAAAGTTAAACGTCTGCCGAAACCGAAATTCGCGGGTGAATGGTCTG
GTCACCGTATGTTCCTGCACGGTCAGCTGAAATCTTGGTCTTCTAACTTCTTCCGTCTGT
TCAACGAAACCCGTGAACTGCTGGAATCTATCAAATCTGACATCCAGCACGCGACCATGC
TGATCTCTTACGTTGAAGAAAAAGGTGGTTACCACCCGCAGCTGCTGTCTCAGTACCGTA
AACTGATGGAACAGCTGCCGGCGCTGCGTACCAAAGTTCTGGACCCGGAAATCGAAATGA
CCCACATGTCTGAAGCGGTTCGTTCTTACATCATGATCCACAAATCTGTTGCGGGTTTCC
TGCCGGACCTGCTGGAATCTCTGGACCGTGACAAAGACCGTGAATTCCTGCTGTCTATCT
TCCCGCGTATCCCGAAAATCGACAAAAAAACCAAAGAAATCGTTGCGTGGGAACTGCCGG
GTGAACCGGAAGAAGGTTACCTGTTCACCGCGAACAACCTGTTCCGTAACTTCCTGGAAA
ACCCGAAACACGTTCCGCGTTTCATGGCGGAACGTATCCCGGAAGACTGGACCCGTCTGC
GTTCTGCGCCGGTTTGGTTCGACGGTATGGTTAAACAGTGGCAGAAAGTTGTTAACCAGC
TGGTTGAATCTCCGGGTGCGCTGTACCAGTTCAACGAATCTTTCCTGCGTCAGCGTCTGC
AGGCGATGCTGACCGTTTACAAACGTGACCTGCAGACCGAAAAATTCCTGAAACTGCTGG
CGGACGTTTGCCGTCCGCTGGTTGACTTCTTCGGTCTGGGTGGTAACGACATCATCTTCA
AATCTTGCCAGGACCCGCGTAAACAGTGGCAGACCGTTATCCCGCTGTCTGTTCCGGCGG
ACGTTTACACCGCGTGCGAAGGTCTGGCGATCCGTCTGCGTGAAACCCTGGGTTTCGAAT
GGAAAAACCTGAAAGGTCACGAACGTGAAGACTTCCTGCGTCTGCACCAGCTGCTGGGTA
ACCTGCTGTTCTGGATCCGTGACGCGAAACTGGTTGTTAAACTGGAAGACTGGATGAACA
ACCCGTGCGTTCAGGAATACGTTGAAGCGCGTAAAGCGATCGACCTGCCGCTGGAAATCT
TCGGTTTCGAAGTTCCGATCTTCCTGAACGGTTACCTGTTCTCTGAACTGCGTCAGCTGG
AACTGCTGCTGCGTCGTAAATCTGTTATGACCTCTTACTCTGTTAAAACCACCGGTTCTC
CGAACCGTCTGTTCCAGCTGGTTTACCTGCCGCTGAACCCGTCTGACCCGGAAAAAAAAA
ACTCTAACAACTTCCAGGAACGTCTGGACACCCCGACCGGTCTGTCTCGTCGTTTCCTGG
ACCTGACCCTGGACGCGTTCGCGGGTAAACTGCTGACCGACCCGGTTACCCAGGAACTGA
AAACCATGGCGGGTTTCTACGACCACCTGTTCGGTTTCAAACTGCCGTGCAAACTGGCGG
CGATGTCTAACCACCCGGGTTCTTCTTCTAAAATGGTTGTTCTGGCGAAACCGAAAAAAG
GTGTTGCGTCTAACATCGGTTTCGAACCGATCCCGGACCCGGCGCACCCGGTTTTCCGTG
TTCGTTCTTCTTGGCCGGAACTGAAATACCTGGAAGGTCTGCTGTACCTGCCGGAAGACA
CCCCGCTGACCATCGAACTGGCGGAAACCTCTGTTTCTTGCCAGTCTGTTTCTTCTGTTG
CGTTCGACCTGAAAAACCTGACCACCATCCTGGGTCGTGTTGGTGAATTCCGTGTTACCG
CGGACCAGCCGTTCAAACTGACCCCGATCATCCCGGAAAAAGAAGAATCTTTCATCGGTA
AAACCTACCTGGGTCTGGACGCGGGTGAACGTTCTGGTGTTGGTTTCGCGATCGTTACCG
TTGACGGTGACGGTTACGAAGTTCAGCGTCTGGGTGTTCACGAAGACACCCAGCTGATGG
CGCTGCAGCAGGTTGCGTCTAAATCTCTGAAAGAACCGGTTTTCCAGCCGCTGCGTAAAG
GTACCTTCCGTCAGCAGGAACGTATCCGTAAATCTCTGCGTGGTTGCTACTGGAACTTCT
ACCACGCGCTGATGATCAAATACCGTGCGAAAGTTGTTCACGAAGAATCTGTTGGTTCTT
CTGGTCTGGTTGGTCAGTGGCTGCGTGCGTTCCAGAAAGACCTGAAAAAAGCGGACGTTC
TGCCGAAAAAAGGTGGTAAAAACGGTGTTGACAAAAAAAAACGTGAATCTTCTGCGCAGG
ACACCCTGTGGGGTGGTGCGTTCTCTAAAAAAGAAGAACAGCAGATCGCGTTCGAAGTTC
AGGCGGCGGGTTCTTCTCAGTTCTGCCTGAAATGCGGTTGGTGGTTCCAGCTGGGTATGC
GTGAAGTTAACCGTGTTCAGGAATCTGGTGTTGTTCTGGACTGGAACCGTTCTATCGTTA
CCTTCCTGATCGAATCTTCTGGTGAAAAAGTTTACGGTTTCTCTCCGCAGCAGCTGGAAA
AAGGTTTCCGTCCGGACATCGAAACCTTCAAAAAAATGGTTCGTGACTTCATGCGTCCGC
CGATGTTCGACCGTAAAGGTCGTCCGGCGGCGGCGTACGAACGTTTCGTTCTGGGTCGTC
GTCACCGTCGTTACCGTTTCGACAAAGTTTTCGAAGAACGTTTCGGTCGTTCTGCGCTGT
TCATCTGCCCGCGTGTTGGTTGCGGTAACTTCGACCACTCTTCTGAACAGTCTGCGGTTG
TTCTGGCGCTGATCGGTTACATCGCGGACAAAGAAGGTATGTCTGGTAAAAAACTGGTTT
ACGTTCGTCTGGCGGAACTGATGGCGGAATGGAAACTGAAAAAACTGGAACGTTCTCGTG
TTGAAGAACAGTCTTCTGCGCAGTAAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTT
TATCTGAAATGTAGGGAGACCCTCAGGTTAAATATTCACTCAGGAAGTTATTACTCAGGA
AGCAAAGAGGATTACA
SEQ AATCAGGAGAGCGTTTTCAATCCTACCTCTGGCGCAGTTGATATGTCAAACAGGTTGCCG
ID TCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAG
NO: CATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAA
78 TCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC
ATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGT
TTCTCCATACCCGTTTTTTTGGGCTAGCAGTAATACGACTCACTATAGGGGTCTCATCTC
GTGTGAGATAGGCGGAGATACGAACTTTAAGAGGAGGATATACCATGCACCATCATCATC
ACCATGCGGAATCTAAACAGATGCAGTGCCGTAAATGCGGTGCGTCTATGAAATACGAAG
TTATCGGTCTGGGTAAAAAATCTTGCCGTTACATGTGCCCGGACTGCGGTAACCACACCT
CTGCGCGTAAAATCCAGAACAAAAAAAAACGTGACAAAAAATACGGTTCTGCGTCTAAAG
CGCAGTCTCAGCGTATCGCGGTTGCGGGTGCGCTGTACCCGGACAAAAAAGTTCAGACCA
TCAAAACCTACAAATACCCGGCGGACCTGAACGGTGAAGTTCACGACTCTGGTGTTGCGG
AAAAAATCGCGCAGGCGATCCAGGAAGACGAAATCGGTCTGCTGGGTCCGTCTTCTGAAT
ACGCGTGCTGGATCGCGTCTCAGAAACAGTCTGAACCGTACTCTGTTGTTGACTTCTGGT
TCGACGCGGTTTGCGCGGGTGGTGTTTTCGCGTACTCTGGTGCGCGTCTGCTGTCTACCG
TTCTGCAGCTGTCTGGTGAAGAATCTGTTCTGCGTGCGGCGCTGGCGTCTTCTCCGTTCG
TTGACGACATCAACCTGGCGCAGGCGGAAAAATTCCTGGCGGTTTCTCGTCGTACCGGTC
AGGACAAACTGGGTAAACGTATCGGTGAATGCTTCGCGGAAGGTCGTCTGGAAGCGCTGG
GTATCAAAGACCGTATGCGTGAATTCGTTCAGGCGATCGACGTTGCGCAGACCGCGGGTC
AGCGTTTCGCGGCGAAACTGAAAATCTTCGGTATCTCTCAGATGCCGGAAGCGAAACAGT
GGAACAACGACTCTGGTCTGACCGTTTGCATCCTGCCGGACTACTACGTTCCGGAAGAAA
ACCGTGCGGACCAGCTGGTTGTTCTGCTGCGTCGTCTGCGTGAAATCGCGTACTGCATGG
GTATCGAAGACGAAGCGGGTTTCGAACACCTGGGTATCGACCCGGGTGCGCTGTCTAACT
TCTCTAACGGTAACCCGAAACGTGGTTTCCTGGGTCGTCTGCTGAACAACGACATCATCG
CGCTGGCGAACAACATGTCTGCGATGACCCCGTACTGGGAAGGTCGTAAAGGTGAACTGA
TCGAACGTCTGGCGTGGCTGAAACACCGTGCGGAAGGTCTGTACCTGAAAGAACCGCACT
TCGGTAACTCTTGGGCGGACCACCGTTCTCGTATCTTCTCTCGTATCGCGGGTTGGCTGT
CTGGTTGCGCGGGTAAACTGAAAATCGCGAAAGACCAGATCTCTGGTGTTCGTACCGACC
TGTTCCTGCTGAAACGTCTGCTGGACGCGGTTCCGCAGTCTGCGCCGTCTCCGGACTTCA
TCGCGTCTATCTCTGCGCTGGACCGTTTCCTGGAAGCGGCGGAATCTTCTCAGGACCCGG
CGGAACAGGTTCGTGCGCTGTACGCGTTCCACCTGAACGCGCCGGCGGTTCGTTCTATCG
CGAACAAAGCGGTTCAGCGTTCTGACTCTCAGGAATGGCTGATCAAAGAACTGGACGCGG
TTGACCACCTGGAATTCAACAAAGCGTTCCCGTTCTTCTCTGACACCGGTAAAAAAAAAA
AAAAAGGTGCGAACTCTAACGGTGCGCCGTCTGAAGAAGAATACACCGAAACCGAATCTA
TCCAGCAGCCGGAAGACGCGGAACAGGAAGTTAACGGTCAGGAAGGTAACGGTGCGTCTA
AAAACCAGAAAAAATTCCAGCGTATCCCGCGTTTCTTCGGTGAAGGTTCTCGTTCTGAAT
ACCGTATCCTGACCGAAGCGCCGCAGTACTTCGACATGTTCTGCAACAACATGCGTGCGA
TCTTCATGCAGCTGGAATCTCAGCCGCGTAAAGCGCCGCGTGACTTCAAATGCTTCCTGC
AGAACCGTCTGCAGAAACTGTACAAACAGACCTTCCTGAACGCGCGTTCTAACAAATGCC
GTGCGCTGCTGGAATCTGTTCTGATCTCTTGGGGTGAATTCTACACCTACGGTGCGAACG
AAAAAAAATTCCGTCTGCGTCACGAAGCGTCTGAACGTTCTTCTGACCCGGACTACGTTG
TTCAGCAGGCGCTGGAAATCGCGCGTCGTCTGTTCCTGTTCGGTTTCGAATGGCGTGACT
GCTCTGCGGGTGAACGTGTTGACCTGGTTGAAATCCACAAAAAAGCGATCTCTTTCCTGC
TGGCGATCACCCAGGCGGAAGTTTCTGTTGGTTCTTACAACTGGCTGGGTAACTCTACCG
TTTCTCGTTACCTGTCTGTTGCGGGTACCGACACCCTGTACGGTACCCAGCTGGAAGAAT
TCCTGAACGCGACCGTTCTGTCTCAGATGCGTGGTCTGGCGATCCGTCTGTCTTCTCAGG
AACTGAAAGACGGTTTCGACGTTCAGCTGGAATCTTCTTGCCAGGACAACCTGCAGCACC
TGCTGGTTTACCGTGCGTCTCGTGACCTGGCGGCGTGCAAACGTGCGACCTGCCCGGCGG
AACTGGACCCGAAAATCCTGGTTCTGCCGGTTGGTGCGTTCATCGCGTCTGTTATGAAAA
TGATCGAACGTGGTGACGAACCGCTGGCGGGTGCGTACCTGCGTCACCGTCCGCACTCTT
TCGGTTGGCAGATCCGTGTTCGTGGTGTTGCGGAAGTTGGTATGGACCAGGGTACCGCGC
TGGCGTTCCAGAAACCGACCGAATCTGAACCGTTCAAAATCAAACCGTTCTCTGCGCAGT
ACGGTCCGGTTCTGTGGCTGAACTCTTCTTCTTACTCTCAGTCTCAGTACCTGGACGGTT
TCCTGTCTCAGCCGAAAAACTGGTCTATGCGTGTTCTGCCGCAGGCGGGTTCTGTTCGTG
TTGAACAGCGTGTTGCGCTGATCTGGAACCTGCAGGCGGGTAAAATGCGTCTGGAACGTT
CTGGTGCGCGTGCGTTCTTCATGCCGGTTCCGTTCTCTTTCCGTCCGTCTGGTTCTGGTG
ACGAAGCGGTTCTGGCGCCGAACCGTTACCTGGGTCTGTTCCCGCACTCTGGTGGTATCG
AATACGCGGTTGTTGACGTTCTGGACTCTGCGGGTTTCAAAATCCTGGAACGTGGTACCA
TCGCGGTTAACGGTTTCTCTCAGAAACGTGGTGAACGTCAGGAAGAAGCGCACCGTGAAA
AACAGCGTCGTGGTATCTCTGACATCGGTCGTAAAAAACCGGTTCAGGCGGAAGTTGACG
CGGCGAACGAACTGCACCGTAAATACACCGACGTTGCGACCCGTCTGGGTTGCCGTATCG
TTGTTCAGTGGGCGCCGCAGCCGAAACCGGGTACCGCGCCGACCGCGCAGACCGTTTACG
CGCGTGCGGTTCGTACCGAAGCGCCGCGTTCTGGTAACCAGGAAGACCACGCGCGTATGA
AATCTTCTTGGGGTTACACCTGGGGTACCTACTGGGAAAAACGTAAACCGGAAGACATCC
TGGGTATCTCTACCCAGGTTTACTGGACCGGTGGTATCGGTGAATCTTGCCCGGCGGTTG
CGGTTGCGCTGCTGGGTCACATCCGTGCGACCTCTACCCAGACCGAATGGGAAAAAGAAG
AAGTTGTTTTCGGTCGTCTGAAAAAATTCTTCCCGTCTTAAGAAATCATCCTTAGCGAAA
GCTAAGGATTTTTTTTATCTGAAATGTAGGGAGACCCTCAGGTTAAATATTCACTCAGGA
AGTTATTACTCAGGAAGCAAAGAGGATTACA
SEQ AATCAGGAGAGCGTTTTCAATCCTACCTCTGGCGCAGTTGATATGTCAAACAGGTTGCCG
ID TCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAG
NO: CATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAA
79 TCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC
ATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGT
TTCTCCATACCCGTTTTTTTGGGCTAGCAGTAATACGACTCACTATAGGGGTCTCATCTC
GTGTGAGATAGGCGGAGATACGAACTTTAAGAGGAGGATATACCATGCACCATCATCATC
ACCATGAAAAACGTATCAACAAAATCCGTAAAAAACTGTCTGCGGACAACGCGACCAAAC
CGGTTTCTCGTTCTGGTCCGATGAAAACCCTGCTGGTTCGTGTTATGACCGACGACCTGA
AAAAACGTCTGGAAAAACGTCGTAAAAAACCGGAAGTTATGCCGCAGGTTATCTCTAACA
ACGCGGCGAACAACCTGCGTATGCTGCTGGACGACTACACCAAAATGAAAGAAGCGATCC
TGCAGGTTTACTGGCAGGAATTCAAAGACGACCACGTTGGTCTGATGTGCAAATTCGCGC
AGCCGGCGTCTAAAAAAATCGACCAGAACAAACTGAAACCGGAAATGGACGAAAAAGGTA
ACCTGACCACCGCGGGTTTCGCGTGCTCTCAGTGCGGTCAGCCGCTGTTCGTTTACAAAC
TGGAACAGGTTTCTGAAAAAGGTAAAGCGTACACCAACTACTTCGGTCGTTGCAACGTTG
CGGAACACGAAAAACTGATCCTGCTGGCGCAGCTGAAACCGGAAAAAGACTCTGACGAAG
CGGTTACCTACTCTCTGGGTAAATTCGGTCAGCGTGCGCTGGACTTCTACTCTATCCACG
TTACCAAAGAATCTACCCACCCGGTTAAACCGCTGGCGCAGATCGCGGGTAACCGTTACG
CGTCTGGTCCGGTTGGTAAAGCGCTGTCTGACGCGTGCATGGGTACCATCGCGTCTTTCC
TGTCTAAATACCAGGACATCATCATCGAACACCAGAAAGTTGTTAAAGGTAACCAGAAAC
GTCTGGAATCTCTGCGTGAACTGGCGGGTAAAGAAAACCTGGAATACCCGTCTGTTACCC
TGCCGCCGCAGCCGCACACCAAAGAAGGTGTTGACGCGTACAACGAAGTTATCGCGCGTG
TTCGTATGTGGGTTAACCTGAACCTGTGGCAGAAACTGAAACTGTCTCGTGACGACGCGA
AACCGCTGCTGCGTCTGAAAGGTTTCCCGTCTTTCCCGGTTGTTGAACGTCGTGAAAACG
AAGTTGACTGGTGGAACACCATCAACGAAGTTAAAAAACTGATCGACGCGAAACGTGACA
TGGGTCGTGTTTTCTGGTCTGGTGTTACCGCGGAAAAACGTAACACCATCCTGGAAGGTT
ACAACTACCTGCCGAACGAAAACGACCACAAAAAACGTGAAGGTTCTCTGGAAAACCCGA
AAAAACCGGCGAAACGTCAGTTCGGTGACCTGCTGCTGTACCTGGAAAAAAAATACGCGG
GTGACTGGGGTAAAGTTTTCGACGAAGCGTGGGAACGTATCGACAAAAAAATCGCGGGTC
TGACCTCTCACATCGAACGTGAAGAAGCGCGTAACGCGGAAGACGCGCAGTCTAAAGCGG
TTCTGACCGACTGGCTGCGTGCGAAAGCGTCTTTCGTTCTGGAACGTCTGAAAGAAATGG
ACGAAAAAGAATTCTACGCGTGCGAAATCCAGCTGCAGAAATGGTACGGTGACCTGCGTG
GTAACCCGTTCGCGGTTGAAGCGGAAAACCGTGTTGTTGACATCTCTGGTTTCTCTATCG
GTTCTGACGGTCACTCTATCCAGTACCGTAACCTGCTGGCGTGGAAATACCTGGAAAACG
GTAAACGTGAATTCTACCTGCTGATGAACTACGGTAAAAAAGGTCGTATCCGTTTCACCG
ACGGTACCGACATCAAAAAATCTGGTAAATGGCAGGGTCTGCTGTACGGTGGTGGTAAAG
CGAAAGTTATCGACCTGACCTTCGACCCGGACGACGAACAGCTGATCATCCTGCCGCTGG
CGTTCGGTACCCGTCAGGGTCGTGAATTCATCTGGAACGACCTGCTGTCTCTGGAAACCG
GTCTGATCAAACTGGCGAACGGTCGTGTTATCGAAAAAACCATCTACAACAAAAAAATCG
GTCGTGACGAACCGGCGCTGTTCGTTGCGCTGACCTTCGAACGTCGTGAAGTTGTTGACC
CGTCTAACATCAAACCGGTTAACCTGATCGGTGTTGACCGTGGTGAAAACATCCCGGCGG
TTATCGCGCTGACCGACCCGGAAGGTTGCCCGCTGCCGGAATTCAAAGACTCTTCTGGTG
GTCCGACCGACATCCTGCGTATCGGTGAAGGTTACAAAGAAAAACAGCGTGCGATCCAGG
CGGCGAAAGAAGTTGAACAGCGTCGTGCGGGTGGTTACTCTCGTAAATTCGCGTCTAAAT
CTCGTAACCTGGCGGACGACATGGTTCGTAACTCTGCGCGTGACCTGTTCTACCACGCGG
TTACCCACGACGCGGTTCTGGTTTTCGAAAACCTGTCTCGTGGTTTCGGTCGTCAGGGTA
AACGTACCTTCATGACCGAACGTCAGTACACCAAAATGGAAGACTGGCTGACCGCGAAAC
TGGCGTACGAAGGTCTGACCTCTAAAACCTACCTGTCTAAAACCCTGGCGCAGTACACCT
CTAAAACCTGCTCTAACTGCGGTTTCACCATCACCACCGCGGACTACGACGGTATGCTGG
TTCGTCTGAAAAAAACCTCTGACGGTTGGGCGACCACCCTGAACAACAAAGAACTGAAAG
CGGAAGGTCAGATCACCTACTACAACCGTTACAAACGTCAGACCGTTGAAAAAGAACTGT
CTGCGGAACTGGACCGTCTGTCTGAAGAATCTGGTAACAACGACATCTCTAAATGGACCA
AAGGTCGTCGTGACGAAGCGCTGTTCCTGCTGAAAAAACGTTTCTCTCACCGTCCGGTTC
AGGAACAGTTCGTTTGCCTGGACTGCGGTCACGAAGTTCACGCGGACGAACAGGCGGCGC
TGAACATCGCGCGTTCTTGGCTGTTCCTGAACTCTAACTCTACCGAATTCAAATCTTACA
AATCTGGTAAACAGCCGTTCGTTGGTGCGTGGCAGGCGTTCTACAAACGTCGTCTGAAAG
AAGTTTGGAAACCGAACGCGTAAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTAT
CTGAAATGTAGGGAGACCCTCAGGTTAAATATTCACTCAGGAAGTTATTACTCAGGAAGC
AAAGAGGATTACA
SEQ AATCAGGAGAGCGTTTTCAATCCTACCTCTGGCGCAGTTGATATGTCAAACAGGTTGCCG
ID TCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAG
NO: CATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAA
80 TCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC
ATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGT
TTCTCCATACCCGTTTTTTTGGGCTAGCAGTAATACGACTCACTATAGGGGTCTCATCTC
GTGTGAGATAGGCGGAGATACGAACTTTAAGAGGAGGATATACCATGCACCATCATCATC
ACCATAAACGTATCAACAAAATCCGTCGTCGTCTGGTTAAAGACTCTAACACCAAAAAAG
CGGGTAAAACCGGTCCGATGAAAACCCTGCTGGTTCGTGTTATGACCCCGGACCTGCGTG
AACGTCTGGAAAACCTGCGTAAAAAACCGGAAAACATCCCGCAGCCGATCTCTAACACCT
CTCGTGCGAACCTGAACAAACTGCTGACCGACTACACCGAAATGAAAAAAGCGATCCTGC
ACGTTTACTGGGAAGAATTCCAGAAAGACCCGGTTGGTCTGATGTCTCGTGTTGCGCAGC
CGGCGCCGAAAAACATCGACCAGCGTAAACTGATCCCGGTTAAAGACGGTAACGAACGTC
TGACCTCTTCTGGTTTCGCGTGCTCTCAGTGCTGCCAGCCGCTGTACGTTTACAAACTGG
AACAGGTTAACGACAAAGGTAAACCGCACACCAACTACTTCGGTCGTTGCAACGTTTCTG
AACACGAACGTCTGATCCTGCTGTCTCCGCACAAACCGGAAGCGAACGACGAACTGGTTA
CCTACTCTCTGGGTAAATTCGGTCAGCGTGCGCTGGACTTCTACTCTATCCACGTTACCC
GTGAATCTAACCACCCGGTTAAACCGCTGGAACAGATCGGTGGTAACTCTTGCGCGTCTG
GTCCGGTTGGTAAAGCGCTGTCTGACGCGTGCATGGGTGCGGTTGCGTCTTTCCTGACCA
AATACCAGGACATCATCCTGGAACACCAGAAAGTTATCAAAAAAAACGAAAAACGTCTGG
CGAACCTGAAAGACATCGCGTCTGCGAACGGTCTGGCGTTCCCGAAAATCACCCTGCCGC
CGCAGCCGCACACCAAAGAAGGTATCGAAGCGTACAACAACGTTGTTGCGCAGATCGTTA
TCTGGGTTAACCTGAACCTGTGGCAGAAACTGAAAATCGGTCGTGACGAAGCGAAACCGC
TGCAGCGTCTGAAAGGTTTCCCGTCTTTCCCGCTGGTTGAACGTCAGGCGAACGAAGTTG
ACTGGTGGGACATGGTTTGCAACGTTAAAAAACTGATCAACGAAAAAAAAGAAGACGGTA
AAGTTTTCTGGCAGAACCTGGCGGGTTACAAACGTCAGGAAGCGCTGCTGCCGTACCTGT
CTTCTGAAGAAGACCGTAAAAAAGGTAAAAAATTCGCGCGTTACCAGTTCGGTGACCTGC
TGCTGCACCTGGAAAAAAAACACGGTGAAGACTGGGGTAAAGTTTACGACGAAGCGTGGG
AACGTATCGACAAAAAAGTTGAAGGTCTGTCTAAACACATCAAACTGGAAGAAGAACGTC
GTTCTGAAGACGCGCAGTCTAAAGCGGCGCTGACCGACTGGCTGCGTGCGAAAGCGTCTT
TCGTTATCGAAGGTCTGAAAGAAGCGGACAAAGACGAATTCTGCCGTTGCGAACTGAAAC
TGCAGAAATGGTACGGTGACCTGCGTGGTAAACCGTTCGCGATCGAAGCGGAAAACTCTA
TCCTGGACATCTCTGGTTTCTCTAAACAGTACAACTGCGCGTTCATCTGGCAGAAAGACG
GTGTTAAAAAACTGAACCTGTACCTGATCATCAACTACTTCAAAGGTGGTAAACTGCGTT
TCAAAAAAATCAAACCGGAAGCGTTCGAAGCGAACCGTTTCTACACCGTTATCAACAAAA
AATCTGGTGAAATCGTTCCGATGGAAGTTAACTTCAACTTCGACGACCCGAACCTGATCA
TCCTGCCGCTGGCGTTCGGTAAACGTCAGGGTCGTGAATTCATCTGGAACGACCTGCTGT
CTCTGGAAACCGGTTCTCTGAAACTGGCGAACGGTCGTGTTATCGAAAAAACCCTGTACA
ACCGTCGTACCCGTCAGGACGAACCGGCGCTGTTCGTTGCGCTGACCTTCGAACGTCGTG
AAGTTCTGGACTCTTCTAACATCAAACCGATGAACCTGATCGGTATCGACCGTGGTGAAA
ACATCCCGGCGGTTATCGCGCTGACCGACCCGGAAGGTTGCCCGCTGTCTCGTTTCAAAG
ACTCTCTGGGTAACCCGACCCACATCCTGCGTATCGGTGAATCTTACAAAGAAAAACAGC
GTACCATCCAGGCGGCGAAAGAAGTTGAACAGCGTCGTGCGGGTGGTTACTCTCGTAAAT
ACGCGTCTAAAGCGAAAAACCTGGCGGACGACATGGTTCGTAACACCGCGCGTGACCTGC
TGTACTACGCGGTTACCCAGGACGCGATGCTGATCTTCGAAAACCTGTCTCGTGGTTTCG
GTCGTCAGGGTAAACGTACCTTCATGGCGGAACGTCAGTACACCCGTATGGAAGACTGGC
TGACCGCGAAACTGGCGTACGAAGGTCTGCCGTCTAAAACCTACCTGTCTAAAACCCTGG
CGCAGTACACCTCTAAAACCTGCTCTAACTGCGGTTTCACCATCACCTCTGCGGACTACG
ACCGTGTTCTGGAAAAACTGAAAAAAACCGCGACCGGTTGGATGACCACCATCAACGGTA
AAGAACTGAAAGTTGAAGGTCAGATCACCTACTACAACCGTTACAAACGTCAGAACGTTG
TTAAAGACCTGTCTGTTGAACTGGACCGTCTGTCTGAAGAATCTGTTAACAACGACATCT
CTTCTTGGACCAAAGGTCGTTCTGGTGAAGCGCTGTCTCTGCTGAAAAAACGTTTCTCTC
ACCGTCCGGTTCAGGAAAAATTCGTTTGCCTGAACTGCGGTTTCGAAACCCACGCGGACG
AACAGGCGGCGCTGAACATCGCGCGTTCTTGGCTGTTCCTGCGTTCTCAGGAATACAAAA
AATACCAGACCAACAAAACCACCGGTAACACCGACAAACGTGCGTTCGTTGAAACCTGGC
AGTCTTTCTACCGTAAAAAACTGAAAGAAGTTTGGAAACCGGAAATCATCCTTAGCGAAA
GCTAAGGATTTTTTTTATCTGAAATGTAGGGAGACCCTCAGGTTAAATATTCACTCAGGA
AGTTATTACTCAGGAAGCAAAGAGGATTACA
SEQ tgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttatt
ID aaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtc
NO: tataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgcc
81 atagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctct
actgtttctccatacccgtttttttgggctagcaccgcctatctcgtgtgagataggcgg
agatacgaactttaagAAGGAGatatacc
SEQ TGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATT
ID AAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTC
NO: TATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCC
82 ATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCT
ACTGTTTCTCCATACCCGTTTTTTTGGGTAGCGGATCCTACCTGAC
SEQ AATCAGGAGAGCGTTTTCAATCCTACCTCTGGCGCAGTTGATATGTCAAACAGGTTTCTA
ID GAGCACAGCTAACACCACGTCGTCCCTATCTGCTGCCCTAGGTCTATGAGTGGTTGCTGG
NO: ATAACTTTACGGGCATGCATAAGGCTCGTAATATATATTCAGGGAGACCACAACGGTTTC
83 CCTCTACAAATAATTTTGTTTAACTTTTACTAGAGCTAGCAGTAATACGACTCACTATAG
GGGTCTCATCTCGTGTGAGATAGGCGGAGATACGAACTTTAAGAGGAGGATATACCA
SEQ GTTTGAGAGATATGTAAATTCAAAGGATAATCAAAC
ID
NO:
84
SEQ actacattttttaagacctaattttgagt
ID
NO:
85
SEQ ctcaaaactcattcgaatctctactctttgtagat
ID
NO:
86
SEQ CTCTAGCAGGCCTGGCAAATTTCTACTGTTGTAGAT
ID
NO:
87
SEQ CCGTCTAAAACTCATTCAGAATTTCTACTAGTGTAGAT
ID
NO:
88
SEQ GTCTAGGTACTCTCTTTAATTTCTACTATTGT
ID
NO:
89
SEQ gttaagttatatagaataatttctactgttgtaga
ID
NO:
90
SEQ gtttaaaaccactttaaaatttctactattgta
ID
NO:
91
SEQ GTTTGAGAATGATGTAAAAATGTATGGTACACAGAAATGTTTTAATACCATATTTTTACA
ID TCACTCTCAAACATACATCTCTTGTTACTGTTTATCGTATCCAGATTAAATTTCACGTTT
NO: TT
92
SEQ CTCTACAACTGATAAAGAATTTCTACTTTTGTAGAT
ID
NO:93
SEQ GTCTGGCCCCAAATTTTAATTTCTACTGTTGTAGAT
ID
NO:
94
SEQ GTCAAAAGACCTTTTTAATTTCTACTCTTGTAGAT
ID
NO:
95
SEQ GTCTAGAGGACAGAATTTTTCAACGGGTGTGCCAATGGCCACTTTCCAGGTGGCAAAGCC
ID CGTTGAGCTTCTACGGAAGTGGCAC
NO:
96
SEQ CGAGGTTCTGTCTTTTGGTCAGGACAACCGTCTAGCTATAAGTGCTGCAGGGGTGTGAGA
ID AACTCCTATTGCTGGACGATGTCTCTTTTAACGAGGCATTAGCAC
NO:
97
SEQ GAACGAGGGACGTTTTGTCTCCAATGATTTTGCTATGACGACCTCGAACTGTGCCTTCAA
ID GTCTGAGGCGAAAAAGAAATGGAAAAAAGTGTCTCATCGCTCTACCTCGTAGTTAGAGG
NO:
98
SEQ AATTACTGATGTTGTGATGAAGG
ID
NO:
99
SEQ TATACCATAAGGATTTAAAGACT
ID
NO:
100
SEQ GTCTTTACTCTCACCTTTCCACCTG
ID
NO:
101
SEQ ATTTGAAGGTATCTCCGATAAGTAAAACGCATCAAAG
ID
NO:
102
SEQ GTTTGAAGATATCTCCGATAAATAAGAAGCATCAAAG
ID
NO:
103
SEQ TTGTTTTAATACCATATTTTTACATCACTCTCAAAC
ID
NO:
104
SEQ AAAGAACGCTCGCTCAGTGTTCTGACCTTTCGAGCGCCTGTTCAGGGCGAAAACCCTGGG
ID AGGCGCTCGAATCATAGGTGGGACAAGGGATTCGCGGCGAAAA
NO:
105
SEQ GTTTGAGAATGATGTAAAAATGTATGGTACACAGAAATGTTTTAATACCATATTTTTACA
ID TCACTCTCAAACATACATCTCTTGTTACTGTTTATCGTATCCAGATTAAATTTCACGTTT
NO: TT
106
SEQ GTCTAGAGGACAGAATTTTTCAACGGGTGTGCCAATGGCCACTTTCCAGGTGGCAAAGCC
ID CGTTGAGCTTCTACGGAAGTGGCAC
NO:
107
SEQ MSIYQEFVNKYSLSKTLRFELIPQGKTLENIKARGLILDDEKRAKDYKKAKQIIDKYHQF
ID FIEEILSSVCISEDLLQNYSDVYFKLKKSDDDNLQKDFKSAKDTIKKQISEYIKDSEKFK
NO: NLFNQNLIDAKKGQESDLILWLKQSKDNGIELFKANSDITDIDEALEIIKSFKGWTTYFK
108 GFHENRKNVYSSNDIPTSIIYRIVDDNLPKFLENKAKYESLKDKAPEAINYEQIKKDLAE
ELTFDIDYKTSEVNQRVFSLDEVFEIANFNNYLNQSGITKFNTIIGGKFVNGENTKRKGI
NEYINLYSQQINDKTLKKYKMSVLFKQILSDTESKSFVIDKLEDDSDVVTTMQSFYEQIA
AFKTVEEKSIKETLSLLFDDLKAQKLDLSKIYFKNDKSLTDLSQQVFDDYSVIGTAVLEY
ITQQIAPKNLDNPSKKEQELIAKKTEKAKYLSLETIKLALEEFNKHRDIDKQCRFEEILA
NFAAIPMIFDEIAQNKDNLAQISIKYQNQGKKDLLQASAEDDVKAIKDLLDQTNNLLHKL
KIFHISQSEDKANILDKDEHFYLVFEECYFELANIVPLYNKIRNYITQKPYSDEKFKLNF
ENSTLANGWDKNKEPDNTAILFIKDDKYYLGVMNKKNNKIFDDKAIKENKGEGYKKIVYK
LLPGANKMLPKVFFSAKSIKFYNPSEDILRIRNHSTHTKNGSPQKGYEKFEFNIEDCRKF
IDFYKQSISKHPEWKDFGFRFSDTQRYNSIDEFYREVENQGYKLTFENISESYIDSVVNQ
GKLYLFQIYNKDFSAYSKGRPNLHTLYWKALFDERNLQDVVYKLNGEAELFYRKQSIPKK
ITHPAKEAIANKNKDNPKKESVFEYDLIKDKRFTEDKFFFHCPITINFKSSGANKFNDEI
NLLLKEKANDVHILSIDRGERHLAYYTLVDGKGNIIKQDTFNIIGNDRMKTNYHDKLAAI
EKDRDSARKDWKKINNIKEMKEGYLSQVVHEIAKLVIEYNAIVVFEDLNFGFKRGRFKVE
KQVYQKLEKMLIEKLNYLVFKDNEFDKTGGVLRAYQLTAPFETFKKMGKQTGIIYYVPAG
FTSKICPVTGFVNQLYPKYESVSKSQEFFSKFDKICYNLDKGYFEFSFDYKNFGDKAAKG
KWTIASFGSRLINFRNSDKNHNWDTREVYPTKELEKLLKDYSIEYGHGECIKAAICGESD
KKFFAKLTSVLNTILQMRNSKTGTELDYLISPVADVNGNFFDSRQAPKNMPQDADANGAY
HIGLKGLMLLGRIKNNQEGKKLNLVIKNEEYFEFVQNRNN
SEQ MSIYQEFVNKYSLSKTLRFELIPQGKTLENIKARGLILDDEKRAKDYKKAKQIIDKYHQF
ID FIEEILSSVCISEDLLQNYSDVYFKLKKSDDDNLQKDFKSAKDTIKKQISEYIKDSEKFK
NO: NLFNQNLIDAKKGQESDLILWLKQSKDNGIELFKANSDITDIDEALEIIKSFKGWTTYFK
109 GFHENRKNVYSSDDIPTSIIYRIVDDNLPKFLENKAKYESLKDKAPEAINYEQIKKDLAE
ELTFDIDYKTSEVNQRVFSLDEVFEIANFNNYLNQSGITKFNTIIGGKFVNGENTKRKGI
NEYINLYSQQINDKTLKKYKMSVLFKQILSDTESKSFVIDKLEDDSDVVTTMQSFYEQIA
AFKTVEEKSIKETLSLLFDDLKAQKLDLSKIYFKNDKSLTDLSQQVFDDYSVIGTAVLEY
ITQQVAPKNLDNPSKKEQDLIAKKTEKAKYLSLETIKLALEEFNKHRDIDKQCRFEEILA
NFAAIPMIFDEIAQNKDNLAQISLKYQNQGKKDLLQASAEEDVKAIKDLLDQTNNLLHRL
KIFHISQSEDKANILDKDEHFYLVFEECYFELANIVPLYNKIRNYITQKPYSDEKFKLNF
ENSTLANGWDKNKEPDNTAILFIKDDKYYLGVMNKKNNKIFDDKAIKENKGEGYKKIVYK
LLPGANKMLPKVFFSAKSIKFYNPSEDILRIRNHSTHTKNGNPQKGYEKFEFNIEDCRKF
IDFYKESISKHPEWKDFGFRFSDTQRYNSIDEFYREVENQGYKLTFENISESYIDSVVNQ
GKLYLFQIYNKDFSAYSKGRPNLHTLYWKALFDERNLQDVVYKLNGEAELFYRKQSIPKK
ITHPAKEAIANKNKDNPKKESVFEYDLIKDKRFTEDKFFFHCPITINFKSSGANKFNDEI
NLLLKEKANDVHILSIDRGERHLAYYTLVDGKGNIIKQDTFNIIGNDRMKTNYHDKLAAI
EKDRDSARKDWKKINNIKEMKEGYLSQVVHEIAKLVIEHNAIVVFEDLNFGFKRGRFKVE
KQVYQKLEKMLIEKLNYLVFKDNEFDKTGGVLRAYQLTAPFETFKKMGKQTGIIYYVPAG
FTSKICPVTGFVNQLYPKYESVSKSQEFFSKFDKICYNLDKGYFEFSFDYKNFGDKAAKG
KWTIASFGSRLINFRNSDKNHNWDTREVYPTKELEKLLKDYSIEYGHGECIKAAICGESD
KKFFAKLTSVLNTILQMRNSKTGTELDYLISPVADVNGNFFDSRQAPKNMPQDADANGAY
HIGLKGLMLLDRIKNNQEGKKLNLVIKNEEYFEFVQNRNN
SEQ MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAE
ID ATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFG
NO: NIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSD
110 VDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGN
LIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAI
LLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYA
GYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELH
AILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEE
VVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFL
SGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKI
IKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWG
RLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSL
HEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRER
MKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDH
IVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNL
TKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKS
KLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRK
MIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDF
ATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVA
YSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPK
YSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVE
QHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
SEQ PKKKRKV
ID
NO:
111
SEQ KRPAATKKAGQAKKKK
ID
NO:
112
SEQ PAAKRVKLD
ID
NO:
113
SEQ RQRRNELKRSP
ID
NO:
114
SEQ NQSSNFGPMKGGNFGGRSSGPYGGGGQYFAKPRNQGGY
ID
NO:
115
SEQ RMRIZFKNKGKDTAELRRRRVEVSVELRKAKKDEQILKRRNV
ID
NO:
116
SEQ VSRKRPRP
ID
NO:
117
SEQ PPKKARED
ID
NO:
118
SEQ PQPKKKPL
ID
NO:
119
SEQ SALIKKKKKMAP
ID
NO:
120
SEQ DRLRR
ID
NO:
121
SEQ PKQKKRK
ID
NO:
122
SEQ RKLKKKIKKL
ID
NO:
123
SEQ REKKKFLKRR
ID
NO:
124
SEQ KRKGDEVDGVDEVAKKKSKK
ID
NO:
125
SEQ RKCLQAGMNLEARKTKK
ID
NO:
126
SEQ ATGGGTAAGATGTATTATCTGGGTTTGGATATAGGCACTAACTCTGTGGGATATGCAGTA
ID ACTGATCCCTCGTATCACTTGTTAAAGTTCAAAGGCGAACCCATGTGGGGAGCACATGTA
NO: TTTGCTGCGGGTAATCAGAGTGCCGAAAGGCGATCTTTCAGAACATCCAGGAGGCGATTA
127 GATAGGAGACAGCAAAGAGTAAAGCTTGTGCAAGAGATCTTTGCTCCTGTCATTTCACCT
ATAGACCCTCGTTTTTTTATAAGATTGCACGAATCGGCTCTATGGAGAGACGATGTTGCC
GAAACAGATAAACATATCTTTTTCAATGATCCCACTTATACAGACAAGGAATACTACTCC
GACTACCCGACAATTCATCATTTGATCGTCGATCTTATGGAGAGCTCTGAAAAGCATGAC
CCCCGACTTGTCTATTTGGCTGTAGCTTGGTTAGTTGCTCATAGAGGTCATTTCTTGAAT
GAAGTAGATAAAGACAATATAGGTGATGTACTTTCTTTTGATGCTTTCTACCCGGAATTT
TTGGCCTTTTTGTCAGACAATGGCGTCAGTCCCTGGGTCTGTGAGTCGAAGGCCCTTCAA
GCTACTCTGCTGTCTAGGAATAGCGTGAACGACAAATATAAAGCATTAAAATCGCTGATA
TTCGGATCGCAAAAACCGGAAGATAACTTTGACGCTAACATCTCTGAAGATGGTTTAATC
CAATTGCTGGCGGGTAAGAAAGTTAAAGTAAACAAACTATTCCCACAAGAGTCCAACGAT
GCTAGCTTTACGTTGAATGATAAAGAAGACGCTATTGAAGAAATTCTAGGTACTTTAACG
CCTGACGAGTGCGAATGGATCGCTCATATTCGCAGATTGTTCGATTGGGCCATCATGAAA
CACGCGCTAAAGGATGGCAGGACGATATCTGAATGAAAAGTGAAGCTATACGAGCAGCAT
CATCATGACTTGACTCAGTTAAAGTACTTTGTGAAGACCTACCTAGCTAAAGAGTATGAT
GATATCTTCAGAAACGTAGACTCCGAGACAACTAAAAATTATGTAGCTTATTCTTACCAT
GTGAAGGAAGTGAAAGGCACATTACCAAAAAATAAAGCAACGCAAGAAGAATTTTGTAAA
TACGTCCTTGGCAAAGTCAAAAACATTGAATGTTCCGAAGCAGACAAGGTTGATTttGAt
GAAAtGAtAcAAcGActtAcGGAcAAttcttttAtGccAAAGcAAGtctcAGGtGAAAAT
AGAGTAATACCATACCAGTTGTACTACTATGAATTAAAGACAATTTTAAACAAAGCCGCC
TCATATCTACCTTTTTTGACACAATGCGGTAAAGATGCTATTTCTAACCAAGACAAATTA
CTGTCTATAATGACATTTCGCATACCATATTTCGTCGGCCCTTTAAGGAAAGATAATTCA
GAACATGCCTGGTTGGAACGTAAAGCGGGTAAAATTTACCCGTGGAACTTTAATGATAAA
GTAGATCTTGATAAATCGGAGGAAGCCTTTATCCGTAGGATGACCAATACTTGCACGTAT
TACCCAGGAGAAGACGTGTTACCATTAGATTCACTTATCTATGAAAAGTTTATGATCTTG
AATGAGATAAACAATATTAGGATTGACGGATACCCCATTTCTGTTGATGTGAAACAACAA
GTATTTGGTTTATTTGAGAAGAAAAGGCGAGTAACAGTTAAGGATATTCAAAATCTACTA
TTATCTCTTGGAGCGTTGGATAAACACGGTAAGCTGACTGGTATTGACACGACAATACAC
TCTAATTATAACACTTATCATCATTTTAAATCTCTTATGGAGCGGGGAGTATTGACCAGA
GATGATGTGGAAAGAATAGTGGAAAGAATGACATATTCTGACGATACTAAGAGGGTCAGA
CTGTGGTTAAATAATAATTATGGAACTCTAACAGCTGACGATGTTAAGCATATCTCAAGA
CTCAGAAAACACGATTTCGGCCGTTTGTCTAAAATGTTTTTGACAGGATTGAAAGGTGTT
CATAAGGAGACAGGCGAGAGAGCAAGTATACTGGATTTTATGTGGAATACTAACGACAAT
TTAATGCAACTACTGTCCGAATGTTACACATTCTCGGATGAGATCACCAAATTACAAGAG
GCCTACTACGCAAAAGCTCAATTATCGCTAAATGACTTCTTGGACTCTATGTATATATCA
AACGCCGTTAAGAGACCTATTTATCGGACCTTAGCGGTAGTAAATGATATTAGAAAGGCA
TGCGGGACGGCACCTAAAAGAATTTTCATCGAGATGGCGCGAGATGGAGAGTCTAAGAAG
AAAAGATCTGTGACTCGTAGAGAGCAAATTAAAAATCTCTATAGATCAATTCGTAAAGAC
TTTCAACAAGAAGTTGATTTTCTGGAAAAGATATTGGAAAATAAGAGTGACGGGCAGCTT
CAGTCTGACGCTTTATATTTGTATTTTGCTCAATTAGGCAGAGACATGTACACAGGTGAT
CCAATCAAATTAGAACATATTAAAGACCAATCTTTTTACAACATTGATCATATTTATCCT
CAATCGATGGTGAAAGATGACAGTTTGGATAACAAGGTACTAGTCCAAAGCGAAATCAAT
GGCGAAAAGAGTTCGCGCTATCCATTAGACGCAGCCATTAGAAACAAAATGAAGCCGTTG
TGGGATGCCTACTATAATCATGGATTAATTTCTCTTAAGAAATACCAGCGTTTGACGAGA
TCTACTCCATTTACGGACGACGAGAAGTGGGATTTTATCAATCGTCAGCTAGTTGAAACT
AGGCAATCTACTAAAGCTTTAGCAATATTGTTAAAGCGTAAGTTTCCAGATACTGAAATA
GTTTACTCAAAGGCTGGACTATCCAGCGATTTTAGACATGAATTCGGCCTGGTTAAGAGT
AGGAATATTAATGATCTACACCATGCTAAAGATGCCTTTCTCGCAATAGTTACTGGGAAC
GTTTATCATGAAAGATTTAATAGAAGATGGTTTATGGTTAACCAGCCATACTCTGTGAAA
ACTAAGACATTGTTTACCCATTCAATTAAGAATGGCAACTTTGTCGCTTGGAATGGAGAA
GAAGATCTTGGACGTATCGTAAAGATGTTGAAACAAAACAAGAACACAATCCACTTCACC
AGGTTTTCCTTTGATAGGAAGGAGGGATTGTTCGATATTCAACCTCTCAAAGCTTCTACC
GGATTGGTTCCACGAAAAGCAGGGTTGGATGTTGTTAAATATGGAGGATACGATAAAAGC
ACTGCCGCGTATTATTTATTAGTACGTTTTACACTCGAGGATAAGAAGACTCAACACAAA
TTGATGATGATTCCTGTTGAAGGTCTCTACAAAGCACGTATTGACCATGATAAAGAGTTT
TTAACAGATTATGCTCAGACCACGATCAGCGAAATTCTTCAAAAGGACAAGCAGAAAGTG
ATCAACATCATGTTCCCTATGGGCACGAGACATATCAAACTGAATTCGATGATTTCTATT
GATGGATTCTATCTTTCTATTGGTGGGAAGAGTAGCAAAGGTAAGTCAGTACTATGTCAT
GCTATGGTGCCATTAATCGTCCCACACAAGATAGAATGTTATATCAAGGCTATGGAATCG
TTTGCAAGAAAATTCAAAGAAAATAATAAATTGAGGATCGTTGAAAAGTTTGATAAAATA
ACTGTTGAAGATAACTTGAACTTATACGAGCTTTTTCTACAAAAGTTGCAACATAACCCA
TATAATAAATTTTTCTCTACACAATTTGATGTGTTGACGAACGGTAGAAGTACATTCACC
AAATTGTCTCCAGAGGAGCAAGTCCAGACTTTACTTAATATACTGAGTATATTTAAAACT
TGTCGTTCTTCTGGGTGTGATTTAAAATCAATAAATGGTTCCGCTCAAGCGGCTAGAATT
ATGATATCCGCTGATTTAACTGGCTTATCAAAAAAGTATTCAGATATTAGATTAGTTGAG
CAAAGCGCATCAGGTCTATTTGTTTCAAAATCTCAAAATCTCTTGGAATACTTGCCAAAA
AAGAAAAGGAAAGTTTAG
SEQ ATGAGTAGTTTAACAAAGTTTACCAATAAATATAGTAAGCAACTAACTATAAAGAACGAA
ID TTGATACCGGTCGGTAAGACTTTGGAAAACATAAAAGAAAATGGGTTGATTGATGGAGAC
NO: GAGCAATTGAATGAGAATTATCAAAAAGCAAAGATAATAGTAGATGATTTTTTGAGAGAC
128 TTTATTAATAAAGCTCTAAATAACACTCAAATTGGTAACTGGAGAGAGCTAGCCGACGCC
TTGAACAAGGAAGATGAGGATAATATTGAGAAATTACAAGATAAGATTAGAGGGATTATC
GTGTCTAAGTTTGAGACTTTTGATCTGTTCAGTTCGTATTCGATTAAAAAGGACGAGAAA
ATCATCGATGATGATAACGATGTGGAAGAAGAGGAGCTAGACCTTGGGAAGAAGACATCT
AGCTTCAAATACATATTCAAGAAAAATTTGTTCAAACTTGTCCTTCCTTCATATTTAAAA
ACAACAAATCAAGATAAGTTAAAAATCATTTCTTCCTTCGATAATTTTAGTACTTATTTT
CGTGGTTTTTTCGAAAACAGGAAAAATATATTCACTAAAAAGCCTATATCTACCTCTATA
GCTTATAGAATTGTTCACGATAATTTCCCAAAATTTCTAGATAATATCAGGTGTTTTAAT
GTTTGGCAAACCGAGTGTCCTCAGTTAATAGTCAAGGCCGACAACTACCTTAAAAGCAAG
AATGTGATTGCAAAAGATAAGTCTTTGGCTAACTATTTTACAGTCGGTGCCTATGATTAT
TTTCTGAGTCAAAATGGTATCGATTTCTATAACAACATTATTGGCGGCTTACCAGCTTTT
GCCGGGCATGAGAAGATTCAGGGTTTGAACGAATTTATCAATCAAGAATGTCAAAAGGAT
TCTGAATTAAAGTCTAAGCTCAAGAATAGGCACGCTTTCAAAATGGCAGTCTTATTCAAA
CAAATCCTTTCAGACAGAGAAAAGTCATTTGTGATTGACGAGTTCGAATCAGACGCTCAG
GTAATTGATGCTGTTAAAAATTTTTACGCGGAACAATGCAAAGATAATAACGTCATATTT
AATTTATTGAATCTGATCAAGAATATTGCTTTTTTGTCGGATGATGAGTTAGACGGCATT
TTCATAGAGGGTAAATACCTGTCCTCTGTGTCTCAAAAATTGTATAGTGATTGGTCAAAG
TTGAGAAATGATATTGAAGATTCGGCTAATTCTAAACAGGGTAACAAAGAATTAGCGAAG
AAAATCAAAACTAACAAGGGTGATGTTGAAAAGGCTATAAGTAAGTACGAGTTCAGTTTA
TCTGAACTAAATTCAATTGTTCATGATAACACAAAATTTTCCGATCTTTTATCATGCACA
TTACATAAAGTTGCAAGTGAAAAATTAGTCAAAGTAAACGAAGGTGATTGGCCAAAACAT
CTAAAAAACAACGAGGAAAAACAGAAGATAAAAGAACCTCTTGACGCTTTATTGGAAATA
TACAATACTCTATTAATATTTAACTGTAAAAGTTTTAACAAAAATGGTAATTTCTATGTC
GACTACGATCGCTGCATTAATGAGTTGTCCAGTGTTGTGTACTTGTATAATAAAACTCGT
AATTATTGTACGAAAAAGCCGTACAACACTGACAAATTTAAGTTGAATTTCAACTCCCCA
CAACTGGGTGAGGGCTTCTCTAAAAGTAAAGAGAATGATTGCCTTACATTATTATTTAAA
AAAGATGATAATTATTATGTCGGAATCATAAGAAAGGGGGCAAAGATCAACTTCGATGAC
ACTCAGGCCATAGCAGACAACACAGATAACTGTATATTCAAAATGAATTATTTTTTGCTG
AAGGATGCTAAAAAATTTATCCCCAAATGTTCAATACAATTAAAAGAGGTTAAGGCCCAT
TTCAAAAAGTCGGAAGATGACTATATTTTGTCCGATAAGGAAAAATTCGCTAGTCCGCTT
GTTATTAAAAAATCCACATTTCTTCTCGCTACGGCTCATGTGAAAGGAAAGAAGGGCAAT
ATTAAGAAATTTCAGAAAGAATACTCCAAAGAAAATCCTACGGAGTATAGAAATAGTCTG
AACGAATGGATAGCATTCTGCAAAGAGTTCTTGAAGACCTATAAAGCTGCCACCATCTTT
GATATTACAACTTTGAAAAAGGCCGAGGAATACGCTGACATTGTGGAATTCTATAAGGAT
GTAGATAATCTTTGTTACAAGTTAGAATTTTGCCCTATCAAAACTTCTTTTATCGAAAAT
CTTATAGATAATGGCGATTTATACCTGTTTAGAATTAATAACAAGGACTTTTCTTCAAAA
AGTACAGGCACGAAAAACTTACACACATTATACTTGCAGGCTATATTTGACGAGCGAAAC
TTAAACAACCCCACGATAATGTTGAATGGAGGTGCAGAGTTATTCTACAGAAAAGAATCT
ATAGAACAGAAAAATCGGATCACGCACAAAGCCGGTAGTATCTTAGTGAATAAAGTGTGC
AAAGATGGTACAAGTCTAGATGACAAAATCCGTAACGAAATTTACCAGTATGAAAACAAA
TTCATTGATACTCTTTCGGACGAAGCTAAAAAGGTTCTGCCAAACGTTATTAAGAAAGAG
GCTACGCATGATATAACAAAAGATAAACGTTTCACTAGCGACAAATTCTTCTTTCATTGT
CCTTTAACAATCAACTACAAGGAAGGTGACACCAAACAATTTAATAATGAAGTGCTCTCA
TTCCTTAGAGGTAACCCCGATATCAATATTATCGGCATTGATAGAGGAGAAAGAAACCTA
ATCTATGTAACAGTCATTAACCAAAAAGGCGAAATATTGGATAGCGTCTCCTTCAATACT
GTCACCAATAAGTCATCGAAGATAGAACAAACTGTTGATTACGAAGAAAAATTGGCCGTT
AGAGAAAAGGAACGTATCGAAGCGAAGAGATCTTGGGATAGCATATCCAAGATTGCCACC
TTGAAGGAGGGTTATCTAAGCGCGATCGTACATGAAATCTGCTTATTAATGATTAAGCAT
AATGCTATTGTCGTGTTAGAAAACCTGAATGCCGGTTTTAAAAGGATTAGAGGTGGTTTG
TCAGAAAAGTCAGTATATCAAAAGTTTGAAAAGATGCTTATTAATAAACTCAACTACTTC
GTTAGCAAGAAAGAAAGTGATTGGAATAAACCGTCAGGTTTGCTCAATGGTCTTCAGTTA
AGTGATCAATTTGAGTCTTTCGAAAAATTAGGAATTCAAAGTGGATTCATTTTTTATGTA
CCAGCCGCGTACACTTCAAAAATTGACCCTACGACCGGATTTGCCAACGTCTTGAATTTG
TCCAAGGTCAGAAATGTTGACGCCATCAAAAGTTTTTTTAGCAACTTCAATGAAATCTCT
TATTCCAAAAAGGAAGCCCTTTTCAAGTTTTCTTTTGACCTAGACTCGTTATCGAAGAAA
GGATTTTCATCTTTCGTAAAGTTTAGCAAGTCCAAGTGGAATGTATACACATTCGGCGAG
AGAATTATCAAGCCCAAGAACAAACAGGGCTATAGAGAAGACAAGAGAATCAACTTGACT
TTTGAGATGAAAAAATTACTCAACGAATACAAGGTTTCATTTGATTTGGAGAACAACTTG
ATTCCCAATTTGACATCAGCTAACTTGAAGGATACGTTCTGGAAGGAGTTATTCTTTATA
TTCAAAACGACATTACAACTGCGTAATAGTGTTACAAACGGTAAAGAAGATGTATTAATC
TCACCTGTAAAGAATGCCAAAGGAGAATTTTTCGTATCCGGTACTCACAATAAGACACTA
CCACAGGATTGCGACGCTAACGGTGCGTATCATATTGCGTTGAAAGGATTAATGATACTT
GAAAGAAATAACCTTGTTCGCGAAGAAAAAGACACCAAGAAGATCATGGCTATTAGCAAT
GTTGATTGGTTTGAATACGTGCAAAAGAGGAGAGGTGTTTTGTAA
SEQ ATGAACAATTATGACGAGTTCACAAAGCTATACCCTATCCAAAAAACTATCAGGTTCGAA
ID TTGAAACCACAAGGGAGAACAATGGAACATCTGGAGACATTCAACTTTTTTGAAGAGGAC
NO: AGAGACAGAGCGGAGAAATACAAAATTTTAAAAGAGGCCATCGATGAATATCACAAAAAG
129 TTTATCGACGAGCATTTAACAAACATGTCTTTGGACTGGAATTCACTTAAACAAATTTCT
GAGAAATATTATAAGTCTCGGGAGGAAAAAGACAAAAAGGTCTTTTTGTCCGAGCAAAAG
AGAATGAGACAAGAAATTGTCTCGGAGTTTAAAAAAGATGATCGGTTCAAAGATTTGTTT
AGCAAGAAATTGTTTTCTGAATTGTTGAAGGAGGAGATATACAAGAAAGGCAACCATCAA
GAAATAGATGCTTTGAAATCGTTTGACAAGTTCAGCGGTTACTTCATTGGTTTACATGAA
AATAGGAAGAACATGTATAGCGACGGCGATGAGATCACCGCTATATCGAATAGAATCGTT
AACGAAAATTTTCCGAAATTTTTGGATAATTTGCAAAAATACCAGGAAGCTAGGAAAAAG
TACCCTGAATGGATAATAAAGGCGGAATCAGCTTTGGTGGCTCACAACATAAAGATGGAT
GAAGTCTTCTCGCTGGAATATTTTAACAAAGTATTAAATCAGGAAGGAATCCAAAGATAC
AACTTAGCCTTGGGTGGATACGTAACCAAATCAGGTGAGAAAATGATGGGCTTAAATGAT
GCACTTAATCTAGCTCACCAATCCGAAAAGTCCTCTAAAGGGAGGATACACATGACACCA
TTGTTTAAGCAAATCCTTTCGGAGAAAGAATCTTTTTCATATATCCCCGATGTTTTCACT
GAGGATAGTCAATTGTTGCCCAGCATTGGTGGATTTTTTGCACAAATAGAAAATGATAAA
GATGGTAACATCTTCGATAGAGCCTTGGAATTGATAAGCTCCTATGCAGAATACGATACG
GAACGAATATACATTAGACAAGCTGACATCAACAGAGTAAGCAATGTTATTTTTGGTGAG
TGGGGAACTTTAGGTGGATTAATGCGGGAGTACAAAGCTGACTCAATCAATGATATTAAT
TTGGAACGTACGTGCAAAAAAGTCGATAAGTGGCTTGATAGTAAGGAGTTTGCTCTGTCG
GATGTACTAGAAGCAATTAAGAGAACAGGAAACAATGATGCATTTAATGAATATATTAGT
AAAATGAGGACGGCTAGAGAAAAGATAGACGCCGCACGTAAGGAAATGAAGTTTATTTCC
GAGAAAATATCTGGCGATGAAGAGTCGATTCACATCATCAAGACCCTACTCGATTCTGTT
CAGCAATTTCTCCATTTTTTTAACCTCTTCAAAGCAAGACAAGACATTCCCTTAGATGGG
GCTTTTTATGCCGAATTTGATGAAGTTCATTCAAAGTTGTTTGCTATTGTTCCTCTTTAC
AATAAGGTCCGTAATTACCTTACTAAAAATAACTTGAACACCAAGAAAATAAAGTTAAAC
TTCAAGAATCCGACTCTTGCCAACGGGTGGGATCAGAATAAAGTTTATGATTATGCTAGC
TTAATATTTCTAAGAGATGGGAATTATTACTTAGGAATCATCAATCCAAAGCGTAAGAAA
AACATTAAATTTGAACAAGGGTCAGGCAATGGCCCATTCTATAGAAAAATGGTGTATAAG
CAAATACCAGGACCTAACAAGAACTTGCCTCGCGTATTTTTAACTTCAACAAAGGGTAAA
AAAGAATATAAACCAAGCAAAGAAATTATTGAAGGTTACGAAGCAGATAAACACATCAGA
GGTGATAAGTTCGATCTGGATTTCTGCCATAAATTGATTGACTTTTTTAAGGAATCTATA
GAAAAACATAAGGACTGGTCCAAATTTAATTTCTACTTCTCACCTACAGAAAGTTATGGT
GACATTTCAGAATTTTATTTAGACGTTGAGAAACAAGGATATAGGATGCATTTTGAAAAT
ATTTCAGCGGAAACCATCGACGAATACGTTGAGAAGGGTGATTTATTCTTGTTCCAAATT
TACAATAAAGACTTCGTTAAAGCTGCAACCGGAAAGAAGGATATGCATACCATATATTGG
AACGCTGCATTCTCGCCAGAAAACTTACAAGATGTCGTTGTAAAGCTTAATGGAGAAGCT
GAGCTGTTCTATAGAGACAAGAGTGATATAAAAGAGATTGTGCATCGGGAAGGTGAAATT
CTGGTGAACAGAACTTACAATGGTCGTACACCCGTTCCAGACAAAATACATAAAAAACTG
ACCGATTATCATAATGGTAGGACAAAGGACTTGGGCGAGGCCAAGGAGTACCTCGATAAA
GTTAGATATTTCAAGGCACACTATGATATTACGAAAGACAGGAGATATTTAAACGATAAA
ATTTACTTTCATGTCCCTTTGACCCTTAACTTTAAAGCTAATGGTAAAAAGAATTTGAAC
AAAATGGTAATTGAGAAGTTTTTATCGGACGAAAAAGCTCACATAATCGGAATCGACCGC
GGAGAGAGAAATTTACTGTATTATAGTATCATCGACAGAAGTGGAAAGATTATTGATCAG
CAATCTTTGAACGTCATTGATGGGTTTGACTATCGGGAAAAGTTAAATCAAAGGGAAATT
GAAATGAAGGATGCGAGACAATCATGGAATGCCATTGGTAAAATTAAAGATCTCAAGGAG
GGGTACTTATCAAAAGCTGTACACGAGATAACTAAAATGGCTATCCAATATAATGCAATT
GTTGTAATGGAAGAATTGAATTATGGTTTTAAACGCGGCAGGTTTAAAGTCGAAAAACAA
ATATACCAAAAGTTTGAAAACATGTTAATTGATAAGATGAACTATCTTGTTTTCAAAGAT
GCACCTGATGAGAGTCCTGGCGGTGTGCTGAACGCCTATCAATTAACAAACCCATTAGAG
TCCTTTGCTAAACTGGGTAAACAAACTGGCATTCTATTTTATGTTCCAGCCGCTTACACC
TCAAAGATCGATCCAACGACCGGTTTTGTAAACTTATTTAATACTTCTTCCAAAACAAAC
GCGCAAGAACGCAAAGAATTCCTACAAAAATTTGAATCAATATCCTATAGCGCAAAAGAT
GGAGGTATATTCGCTTTCGCTTTTGACTACAGAAAGTTTGGCACTTCCAAGACAGATCAT
AAAAATGTGTGGACCGCTTATACCAACGGAGAAAGGATGCGTTATATTAAAGAAAAAAAG
AGGAACGAACTATTTGATCCATCGAAAGAAATTAAAGAAGCTTTGACAAGCAGCGGAATC
AAATATGATGGAGGTCAAAACATACTTCCAGATATTCTCAGATCTAATAATAACGGTCTT
ATTTACACGATGTATTCATCTTTTATCGCTGCCATCCAAATGCGTGTGTATGATGGCAAG
GAAGATTATATTATATCTCCTATTAAAAATTCAAAGGGTGAATTTTTTCGCACGGATCCA
AAAAGAAGAGAGCTTCCAATTGACGCCGATGCTAACGGTGCTTACAATATTGCATTGCGT
GGTGAACTTACTATGAGAGCCATCGCCGAAAAGTTTGATCCGGACAGTGAAAAAATGGCG
AAATTGGAGCTAAAGCACAAGGATTGGTTTGAATTCATGCAGACCCGTGGCGATTGA
SEQ ATGACTAAAACGTTCGACTCCGAGTTTTTTAATCTCTATTCCTTGCAAAAGACCGTTAGG
ID TTTGAATTGAAACCAGTTGGTGAAACTGCCTCATTTGTCGAAGACTTTAAAAACGAGGGA
NO: TTGAAAAGAGTGGTTAGTGAAGATGAAAGAAGGGCAGTAGACTATCAAAAGGTTAAAGAA
130 ATCATTGACGATTACCACAGAGATTTTATAGAAGAATCTCTGAACTATTTTCCAGAGCAG
GTTTCAAAAGATGCTCTAGAGCAAGCGTTTCATTTGTATCAAAAGTTGAAAGCAGCGAAG
GTGGAAGAAAGGGAAAAAGCTTTAAAAGAATGGGAAGCATTACAGAAAAAATTGCGAGAA
AAAGTCGTCAAATGTTTCAGCGACTCTAATAAAGCTCGCTTTTCTAGAATCGATAAAAAA
GAATTGATTAAGGAAGATTTAATAAATTGGCTGGTAGCACAAAACAGAGAGGATGATATT
CCTACTGTTGAAACGTTCAATAATTTTACTACTTACTTCACTGGTTTCCATGAGAACAGG
AAGAATATTTACTCTAAAGATGATCACGCTACTGCTATAAGTTTTAGGTTGATTCACGAA
AACTTGCCTAAATTTTTTGACAATGTCATCAGTTTTAACAAGTTGAAAGAAGGTTTCCCG
GAATTAAAATTCGACAAAGTTAAAGAAGATTTAGAAGTAGATTACGACTTGAAGCATGCG
TTTGAAATTGAATATTTCGTTAATTTCGTCACACAAGCTGGTATCGACCAATATAATTAC
CTGCTTGGAGGCAAAACTCTAGAAGACGGTACGAAGAAACAAGGAATGAATGAACAGATT
AATTTATTTAAGCAACAACAAACTCGCGATAAAGCTAGACAGATTCCAAAACTGATTCCA
CTTTTCAAACAGATTCTATCTGAGAGAACTGAATCTCAGAGTTTTATCCCTAAGCAGTTC
GAGTCTGATCAGGAACTATTCGATTCCCTGCAGAAATTGCATAACAACTGTCAAGATAAG
TTTACCGTTTTGCAACAGGCGATCTTGGGATTGGCTGAGGCAGATCTTAAAAAGGTCTTT
ATTAAAACTAGTGATCTAAACGCATTGTCTAACACTATTTTTGGAAATTATTCTGTGTTC
TCAGACGCGCTCAATTTATATAAAGAGTCGCTAAAAACTAAAAAGGCTCAAGAAGCTTTT
GAAAAGTTGCCTGCACATAGTATTCATGATTTAATCCAATACTTAGAACAATTTAATTCG
TCTCTCGATGCTGAAAAGCAACAGTCTACCGATACTGTATTAAACTACTTTATTAAAACC
GACGAATTATATAGTCGTTTCATTAAATCCACCTCTGAGGCATTCACCCAAGTACAACCT
CTCTTTGAACTGGAAGCTTTGAGCTCCAAAAGAAGACCCCCAGAAAGTGAAGATGAGGGG
GCTAAAGGCCAAGAAGGTTTCGAACAAATTAAGAGAATCAAAGCTTATCTAGACACTCTA
ATGGAGGCTGTCCACTTTGCTAAGCCTTTGTATCTTGTCAAGGGTAGAAAGATGATAGAG
GGTCTAGACAAGGATCAAAGCTTCTACGAAGCGTTTGAAATGGCCTACCAGGAGTTGGAG
TCTTTAATCATCCCCATTTACAATAAGGCCAGATCTTACCTGTCTAGGAAGCCATTTAAA
GCGGATAAATTCAAAATTAATTTTGACAATAATACACTTCTATCTGGGTGGGATGCTAAC
AAGGAGACGGCTAACGCCAGCATATTGTTTAAGAAGGATGGTTTATACTACCTGGGAATC
ATGCCAAAAGGCAAAACTTTCTTGTTCGATTATTTCGTTAGTTCAGAAGATTCTGAAAAG
TTGAAACAACGGAGACAGAAAACCGCAGAGGAAGCGCTCGCACAGGATGGAGAATCCTAT
TTTGAAAAAATACGGTATAAACTCCTACCAGGTGCTAGTAAGATGTTGCCAAAGGTATTT
TTTAGCAATAAAAATATTGGGTTTTACAATCCCTCAGATGATATTCTACGAATTCGGAAT
ACGGCCTCTCATACTAAGAATGGTACTCCCCAGAAGGGTCATTCCAAGGTAGAATTTAAC
TTGAATGACTGTCACAAAATGATTGATTTTTTTAAATCTTCCATACAGAAACATCCCGAG
TGGGGATCCTTTGGTTTCACTTTTTCTGATACGTCGGACTTTGAAGATATGAGTGCTTTC
TACCGAGAAGTTGAAAATCAAGGTTACGTTATAAGTTTTGATAAAATAAAAGAAACTTAC
ATTCAGTCTCAAGTTGAGCAAGGTAACTTATATTTATTTCAAATTTACAACAAAGATTTT
AGTCCGTATTCAAAGGGAAAGCCAAACCTGCACACTTTATACTGGAAAGCTCTGTTTGAA
GAGGCTAATTTGAATAACGTAGTGGCTAAGCTAAACGGCGAAGCAGAAATCTTTTTCAGA
AGACACAGTATCAAAGCATCTGATAAAGTGGTACATCCTGCTAATCAAGCTATAGATAAT
AAGAATCCCCATACTGAGAAGACGCAGTCCACATTTGAATATGACTTGGTCAAAGACAAA
AGATATACCCAAGACAAATTTTTTTTTCATGTACCGATATCTTTAAACTTTAAGGCTCAG
GGCGTTTCAAAGTTTAATGATAAGGTAAATGGATTCTTAAAGGGCAATCCCGACGTTAAT
ATAATCGGTATAGATCGAGGTGAGAGACATCTTTTATACTTTACCGTGGTGAATCAAAAA
GGAGAAATATTAGTGCAAGAGTCCTTGAATACATTAATGTCTGACAAGGGTCATGTCAAC
GATTATCAACAGAAATTGGACAAGAAGGAACAGGAAAGGGACGCTGCCAGGAAGTCCTGG
ACGACAGTAGAAAATATTAAAGAATTAAAAGAAGGTTATTTATCACATGTGGTTCATAAA
CTTGCACATTTAATCATCAAATATAACGCAATAGTGTGCTTGGAAGATCTTAATTTTGGC
TTCAAGAGGGGTAGGTTCAAGGTCGAAAAACAGGTCTACCAGAAGTTCGAGAAAGCTCTG
ATCGATAAATTGAATTATCTTGTTTTCAAAGAAAAAGAATTAGGAGAAGTTGGTCATTAT
CTTACAGCATACCAACTCACTGCACCATTTGAAAGCTTCAAAAAGCTAGGCAAGCAATCT
GGGATTTTGTTCTATGTTCCGGCTGATTATACATCAAAGATAGATCCTACCACAGGCTTT
GTAAATTTTTTAGATCTTAGGTACCAATCCGTTGAAAAAGCTAAACAGTTGCTGTCCGAT
TTTAATGCGATAAGATTTAATAGTGTTCAGAATTATTTTGAGTTCGAAATTGATTATAAA
AAATTGACACCAAAACGTAAAGTAGGAACACAATCTAAATGGGTTATTTGTACCTATGGA
GATGTTAGATACCAAAACAGAAGAAATCAGAAAGGTCACTGGGAAACTGAAGAAGTTAAC
GTTACTGAAAAACTTAAAGCTCTATTTGCGAGCGATTCAAAAACGACGACGGTGATCGAT
TATGCAAATGATGATAACCTTATTGATGTAATTCTGGAACAAGATAAGGCATCATTTTTT
AAAGAACTACTATGGTTGTTAAAGCTAACCATGACCCTAAGGCACTCCAAGATAAAGTCA
GAGGATGATTTTATCCTCTCTCCAGTGAAAAACGAACAAGGTGAGTTTTACGACTCAAGA
AAGGCGGGTGAAGTCTGGCCTAAGGATGCTGATGCCAATGGAGCTTATCACATCGCTCTG
AAGGGGCTATGGAACTTACAGCAAATTAACCAATGGGAAAAAGGTAAAACTTTAAACCTC
GCCATAAAGAACCAGGATTGGTTCAGCTTTATCCAAGAAAAACCATATCAAGAATAA
SEQ ATGCACACAGGAGGTCTACTCTCGATGGATGCTAAGGAATTTACCGGTCAATATCCGCTG
ID TCCAAAACTTTGCGTTTTGAGCTTAGACCTATTGGCCGAACGTGGGATAACCTAGAGGCT
NO: TCTGGTTATTTGGCGGAAGATAGACATAGAGCTGAGTGTTATCCCCGAGCTAAAGAATTG
131 CTGGATGATAACCACAGGGCGTTCCTGAATAGAGTTCTACCGCAAATCGATATGGATTGG
CATCCAATTGCTGAAGCTTTCTGCAAGGTGCACAAAAATCCAGGTAATAAAGAATTGGCT
CAGGATTATAATTTGCAGCTTAGTAAGAGAAGAAAAGAAATTTCCGCTTATTTGCAGGAT
GCTGATGGATACAAGGGGTTGTTCGCGAAACCTGCCCTGGACGAAGCTATGAAAATAGCT
AAGGAAAACGGCAATGAATCTGATATTGAAGTTTTGGAAGCCTTCAATGGATTTTCCGTT
TATTTCACTGGTTATCATGAGAGTAGGGAGAATATATACTCAGACGAAGATATGGTATCC
GTCGCCTATCGCATAACTGAAGATAATTTTCCAAGGTTCGTGTCGAACGCGTTAATTTTT
GATAAACTAAATGAATCGCACCCGGATATTATTTCGGAAGTGTCCGGTAATCTGGGGGTA
GACGATATTGGTAAATATTTTGATGTGTCCAACTACAATAATTTCCTTAGTCAAGCAGGA
ATTGATGACTACAACCATATTATAGGAGGGCATACAACTGAAGACGGTCTCATTCAAGCT
TTTAACGTAGTGTTAAACCTAAGGCACCAAAAAGACCCAGGTTTTGAGAAAATTCAATTT
AAGCAACTCTACAAGCAGATACTGAGCGTTAGGACTAGTAAGTCATATATCCCAAAGCAA
TTCGATAACTCAAAGGAAATGGTCGACTGTATATGCGACTACGTCTCAAAAATAGAAAAA
TCTGAAACAGTAGAAAGAGCTCTGAAATTGGTAAGAAATATATCTTCTTTTGATTTAAGA
GGTATTTTCGTAAATAAAAAAAACCTTCGAATTTTGTCTAATAAGTTAATTGGAGACTGG
GACGCAATAGAGACAGCTTTGATGCACAGTTCCAGCAGTGAAAACGATAAGAAATCAGTG
TATGACTCTGCAGAGGCATTCACCCTTGATGATATCTTCAGTTCTGTGAAAAAGTTCAGC
GACGCCTCCGCTGAGGATATAGGAAACCGCGCTGAAGACATATGTCGTGTTATCTCAGAA
ACAGCTCCTTTCATTAACGACTTAAGGGCTGTAGATTTGGATTCTTTAAATGATGACGGC
TATGAAGCGGCCGTGTCTAAAATACGGGAATCTCTTGAACCCTACATGGATCTATTTCAC
GAATTGGAGATCTTTAGCGTGGGTGATGAGTTTCCTAAATGTGCTGCCTTTTATAGCGAG
TTGGAAGAGGTCTCAGAACAACTGATTGAAATCATTCCTTTATTTAACAAAGCAAGAAGT
TTTTGCACAAGGAAAAGGTATTCAACCGACAAAATCAAAGTCAATTTAAAATTCCCTACT
CTGGCAGATGGATGGGATCTAAATAAAGAAAGGGATAACAAAGCCGCAATTCTAAGAAAA
GACGGTAAATACTACCTGGCAATTTTAGACATGAAGAAAGATCTCAGTAGTATTCGTACG
AGCGATGAGGACGAGTCTTCTTTTGAAAAGATGGAATATAAATTGCTCCCTTCTCCTGTG
AAAATGCTTCCAAAAATTTTTGTTAAATCGAAAGCCGCCAAAGAAAAGTACGGGTTGACC
GATAGAATGTTAGAATGCTACGATAAAGGTATGCATAAGTCGGGTAGTGCTTTTGATTTG
GGTTTTTGTCATGAATTGATCGATTACTATAAGCGCTGCATTGCCGAGTACCCAGGCTGG
GATGTTTTCGACTTTAAATTTCGTGAGACAAGCGATTACGGATCCATGAAAGAATTTAAT
GAAGACGTCGCTGGCGCAGGTTACTATATGTCACTTAGAAAGATTCCATGTTCCGAAGTT
TATCGTTTACTGGACGAGAAGTCAATTTACTTGTTTCAAATATATAATAAGGATTATAGC
GAAAACGCACATGGGAATAAGAATATGCATACGATGTATTGGGAGGGCTTGTTCTCACCA
CAAAATTTGGAATCACCAGTCTTCAAATTGTCCGGAGGCGCAGAACTTTTTTTCAGAAAG
TCATCTATTCCTAATGACGCTAAAACGGTACATCCGAAAGGTTCAGTTCTTGTTCCCAGA
AACGACGTCAATGGTAGAAGAATACCAGACTCGATCTACAGAGAGTTGACAAGGTATTTT
AACCGTGGGGATTGCAGGATCAGTGATGAAGCTAAGTCTTACCTGGACAAGGTCAAGACA
AAAAAAGCGGACCATGACATTGTTAAGGATAGAAGATTTACTGTAGATAAGATGATGTTC
CATGTTCCGATTGCCATGAATTTTAAAGCTATAAGTAAACCAAATCTTAATAAGAAAGTT
ATTGATGGCATAATAGATGATCAAGATTTGAAAATCATCGGTATCGATCGTGGTGAGAGA
AATCTTATTTATGTGACCATGGTCGATAGGAAGGGGAATATATTGTATCAAGACAGTCTT
AATATTTTAAATGGATACGATTACCGCAAAGCTTTAGACGTGAGGGAATATGATAACAAA
GAAGCTAGAAGGAATTGGACTAAAGTAGAAGGTATTAGAAAAATGAAAGAAGGTTATTTA
TCTTTAGCTGTTAGTAAATTGGCCGATATGATCATCGAAAATAATGCTATAATCGTAATG
GAAGATTTGAATCACGGGTTTAAGGCAGGTCGTTCCAAAATTGAAAAGCAGGTGTATCAA
AAATTCGAATCAATGTTAATCAACAAGTTAGGATACATGGTGCTAAAAGACAAGTCCATT
GACCAGTCTGGTGGAGCCCTTCATGGTTACCAATTAGCCAATCATGTTACGACCTTAGCT
AGCGTGGGTAAACAATGTGGAGTAATTTTTTACATACCTGCAGCTTTTACTTCGAAGATT
GATCCCACCACGGGCTTTGCTGATTTATTCGCTCTCTCTAATGTGAAGAATGTCGCTTCT
ATGAGAGAGTTCTTCTCCAAAATGAAGTCAGTAATATATGACAAGGCGGAAGGCAAATTC
GCCTTTACATTTGATTATTTGGATTATAACGTTAAAAGCGAATGTGGACGTACCTTATGG
ACTGTGTATACAGTTGGTGAACGCTTCACCTACTCTAGAGTAAACCGAGAGTATGTTCGG
AAAGTCCCAACAGATATCATCTATGATGCATTACAAAAAGCTGGTATTAGCGTCGAAGGT
GACCTTAGAGATAGAATCGCGGAAAGCGACGGTGACACATTAAAGTCTATATTCTACGCT
TTTAAATACGCGTTGGATATGAGAGTCGAAAACAGAGAGGAAGACTATATACAGTCACCT
GTGAAGAATGCTTCTGGTGAGTTCTTTTGTTCAAAAAACGCCGGAAAGTCTTTGCCGCAG
GATTCAGATGCAAATGGTGCCTATAATATAGCTCTGAAAGGGATCCTACAACTCAGAATG
TTGAGCGAACAATACGATCCAAATGCAGAATCGATTAGATTGCCACTTATAACTAACAAG
GCATGGTTAACTTTTATGCAATCCGGTATGAAAACTTGGAAGAATTAA
SEQ ATGGATTCTCTTAAGGATTTCACTAATTTATATCCAGTCTCGAAAACATTGCGGTTCGAA
ID TTGAAACCAGTTGGGAAAACTCTAGAAAACATTGAAAAAGCCGGTATATTGAAAGAAGAT
NO: GAACACAGAGCGGAATCCTACCGCCGGGTAAAAAAGATAATTGACACATACCATAAAGTG
132 TTTATTGACAGCTCCTTAGAGAACATGGCTAAAATGGGGATAGAAAATGAAATCAAGGCT
ATGCTGCAGTCTTTTTGTGAACTCTATAAGAAAGACCACAGGACAGAAGGAGAAGATAAA
GCTCTTGATAAAATTAGAGCTGTTCTTAGAGGTTTAATCGTTGGGGCTTTCACTGGTGTA
TGTGGAAGACGAGAAAACACAGTACAAAATGAAAAGTACGAGAGTTTGTTCAAAGAAAAA
TTGATAAAGGAAATTTTGCCAGATTTCGTGTTGTCCACCGAGGCTGAGTCTCTTCCATTC
AGCGTTGAAGAAGCAACAAGGAGCTTAAAAGAGTTTGACTCATTCACTTCTTATTTTGCT
GGTTTTTACGAAAATAGAAAGAATATTTATTCCACGAAACCGCAAAGTACTGCGATAGCC
TACAGATTAATTCATGAAAACTTGCCTAAATTTATAGATAATATTTTGGTCTTCCAGAAG
ATTAAAGAACCAATCGCTAAAGAACTTGAGCACATAAGAGCAGATTTTAGCGCAGGCGGA
TATATCAAAAAAGATGAACGGCTAGAAGACATATTCTCATTAAATTACTACATTCATGTC
CTTTCTCAAGCTGGTATAGAAAAATATAATGCTTTAATCGGGAAGATAGTGACGGAAGGT
GATGGTGAAATGAAAGGTCTTAATGAACATATTAACTTATATAACCAACAGAGGGGTCGA
GAGGATAGGTTGCCCTTGTTTAGGCCTCTATACAAGCAAATCCTGTCCGATAGAGAGCAA
TTGTCTTATTTACCTGAATCATTTGAAAAAGATGAAGAGCTGCTTAGAGCACTTAAGGAA
TTTTACGATCACATCGCCGAAGACATCTTGGGTAGAACACAGCAATTGATGACTTCAATT
TCTGAATACGACTTGTCCCGTATTTATGTCAGAAATGATTCTCAACTTACAGACATCTCG
AAGAAAATGCTAGGAGATTGGAACGCCATTTATATGGCTAGAGAACGAGCCTACGACCAC
GAACAGGCTCCTAAACGTATTACTGCTAAATACGAACGTGATAGAATCAAGGCCTTAAAA
GGTGAAGAGTCAATTTCATTGGCGAATCTGAACAGCTGTATAGCTTTCTTGGACAATGTA
AGGGATTGTCGAGTTGACACATACCTATCAACTTTGGGGCAGAAAGAGGGTCCTCATGGC
TTAAGTAACTTGGTGGAAAACGTCTTCGCCTCATATCATGAAGCAGAACAGTTATTGTCG
TTTCCTTACCCCGAAGAGAACAACCTTATTCAGGACAAAGACAATGTAGTTTTGATCAAA
AACCTATTGGATAATATAAGTGATTTACAACGTTTCCTTAAACCTTTGTGGGGAATGGGC
GATGAACCTGACAAAGACGAAAGGTTTTACGGTGAATACAACTATATTAGAGGAGCGCTT
GACCAGGTAATACCTTTGTACAATAAAGTAAGGAACTACTTGACTCGTAAACCATATTCT
ACTAGAAAAGTTAAATTGAACTTTGGTAATTCACAGCTGCTGAGTGGTTGGGATCGTAAT
AAAGAAAAAGATAACTCCTGTGTTATCTTGCGAAAAGGACAAAACTTTTACTTGGCAATT
ATGAACAACCGTCACAAAAGGTCCTTCGAGAACAAAGTTCTGCCTGAATACAAAGAAGGT
GAACCATATTTTGAAAAAATGGACTATAAATTCCTGCCAGATCCTAATAAAATGTTGCCT
AAGGTCTTCTTGTCTAAAAAAGGTATAGAAATATATAAACCATCCCCGAAGTTGCTGGAG
CAATATGGTCATGGAACGCACAAAAAAGGTGACACTTTTAGTATGGATGACTTGCACGAG
TTGATTGATTTTTTTAAACATTCCATTGAAGCGCACGAAGATTGGAAACAATTTGGTTTC
AAGTTCTCTGACACAGCCACTTACGAAAATGTATCGTCCTTTTATAGAGAAGTGGAAGAT
CAGGGTTATAAACTGTCATTCCGTAAGGTTAGTGAAAGCTATGTGTACTCGTTGATCGAT
CAAGGGAAGCTTTATCTTTTTCAAATCTATAATAAAGATTTCTCTCCTTGTTCAAAGGGC
ACACCTAATCTTCATACACTATACTGGAGAATGCTTTTCGATGAAAGAAATTTGGCTGAT
GTGATCTATAAATTAGACGGTAAAGCTGAGATTTTTTTCAGAGAGAAATCCCTGAAAAAC
GACCATCCAACTCATCCGGCAGGTAAACCGATTAAAAAGAAATCCCGGCAAAAAAAGGGC
GAAGAGAGTTTATTCGAGTATGATTTAGTTAAGGACAGACATTATACAATGGACAAATTT
CAATTTCATGTGCCCATTACTATGAACTTTAAGTGTAGTGCAGGGTCTAAGGTTAATGAT
ATGGTAAACGCACATATTAGAGAAGCTAAAGATATGCACGTCATCGGTATTGATCGCGGA
GAAAGAAATTTACTTTACATTTGCGTTATCGATTCTAGGGGCACCATCTTGGATCAAATC
TCTTTGAACACTATAAATGATATTGACTATCATGATCTACTAGAGAGTCGGGATAAAGAC
AGGCAACAAGAAAGAAGAAATTGGCAAACAATTGAAGGTATTAAAGAATTAAAGCAAGGC
TATCTAAGCCAGGCTGTACACAGAATTGCCGAATTAATGGTAGCATATAAAGCTGTCGTA
GCTCTAGAAGACTTGAACATGGGTTTCAAAAGAGGGCGCCAGAAGGTCGAAAGTAGTGTT
TATCAACAATTTGAAAAACAGTTAATAGATAAGTTGAATTATCTAGTGGATAAAAAAAAG
CGTCCTGAGGACATTGGCGGTTTATTAAGAGCCTACCAATTCACTGCGCCATTTAAATCG
TTCAAAGAAATGGGTAAACAAAACGGTTTTCTATTCTACATCCCCGCATGGAATACCTCA
AATATAGATCCAACTACCGGTTTCGTCAACTTATTTCATGCTCAATATGAGAATGTGGAC
AAAGCAAAATCATTCTTTCAAAAATTTGATAGCATTAGCTACAATCCTAAAAAAGATTGG
TTTGAATTTGCGTTCGATTATAAAAATTTCACCAAGAAGGCTGAAGGTTCCAGATCTATG
TGGATATTGTGCACCCACGGAAGTAGAATTAAGAACTTCCGTAATTCACAGAAAAACGGC
CAGTGGGACAGCGAAGAATTCGCCCTAACCGAAGCTTTCAAAAGTCTTTTCGTAAGATAC
GAGATAGACTATACAGCTGATCTAAAGACAGCTATTGTGGATGAGAAGCAAAAAGACTTC
TTTGTCGACCTTCTTAAGTTGTTCAAGTTAACTGTGCAGATGAGAAATAGTTGGAAGGAA
AAAGACCTAGATTACTTGATTAGCCCAGTCGCTGGTGCAGATGGCAGATTTTTTGATACA
CGTGAAGGCAATAAATCACTACCAAAAGACGCGGACGCTAATGGCGCATACAACATCGCA
TTGAAGGGTTTGTGGGCTCTCAGGCAGATTAGGCAGACAAGTGAGGGTGGTAAGCTTAAG
CTGGCGATTTCTAATAAGGAATGGTTACAGTTTGTTCAAGAAAGATCCTACGAAAAAGAT
TAA
SEQ ATGAACAATGGTACTAATAATTTTCAAAACTTCATAGGGATTTCTAGCCTTCAAAAGACA
ID TTGAGAAATGCTTTAATTCCAACAGAAACGACTCAACAATTCATAGTGAAAAATGGTATT
NO: ATAAAAGAAGACGAGTTGCGTGGCGAGAATAGACAAATTTTGAAAGATATCATGGATGAC
133 TACTACAGAGGGTTCATCTCCGAAACATTGTCTTCTATTGACGACATTGACTGGACCAGC
TTATTCGAAAAAATGGAAATACAGCTGAAGAACGGAGATAACAAGGACACTCTTATAAAG
GAGCAAACGGAATATAGAAAGGCTATACACAAAAAGTTTGCTAATGACGATAGATTTAAA
AACATGTTTAGTGCGAAGTTAATTTCTGATATTCTACCCGAGTTTGTCATTCATAATAAT
AACTACTCTGCATCTGAAAAAGAGGAGAAGACCCAGGTTATAAAGTTGTTTTCAAGATTT
GCCACATCATTTAAAGACTACTTCAAGAACAGGGCGAATTGCTTCTCTGCTGATGATATT
AGCTCTTCCAGCTGTCATAGAATTGTTAACGATAATGCCGAAATTTTTTTTAGTAATGCC
TTGGTATATAGACGCATAGTCAAGTCACTAAGCAATGATGATATAAACAAGATTAGTGGT
GATATGAAAGATAGCCTTAAAGAAATGAGCCTTGAAGAGATATATTCATATGAGAAGTAC
GGTGAATTTATAACTCAAGAAGGAATTTCTTTTTATAACGATATTTGTGGTAAGGTTAAT
TCTTTTATGAATTTGTATTGCCAGAAGAACAAGGAAAATAAGAATCTATATAAACTACAA
AAGTTGCATAAACAGATTTTGTGTATAGCTGATACATCCTACGAAGTTCCGTATAAATTT
GAATCTGATGAGGAAGTTTATCAATCGGTAAACGGTTTTCTTGACAACATTTCCAGCAAA
CATATCGTTGAGAGACTACGTAAAATTGGAGACAACTATAATGGTTACAATCTAGATAAA
ATATACATAGTGTCCAAGTTTTATGAGTCTGTCTCTCAAAAGACATATCGTGATTGGGAG
ACCATTAATACTGCACTTGAAATTCATTATAACAACATATTGCCTGGTAACGGGAAGAGT
AAAGCTGATAAGGTTAAAAAGGCCGTCAAAAACGACTTGCAAAAGTCTATTACCGAGATA
AATGAATTAGTGTCAAACTACAAACTATGCTCAGATGATAATATTAAAGCGGAAACATAC
ATCCACGAAATTTCCCACATACTGAATAACTTTGAAGCTCAGGAGCTTAAATATAACCCG
GAAATACACTTGGTTGAGAGCGAGTTAAAAGCATCTGAGTTGAAAAATGTATTAGACGTC
ATCATGAATGCGTTTCATTGGTGTTCAGTTTTCATGACTGAAGAATTAGTCGACAAAGAT
AACAATTTTTATGCCGAATTAGAGGAAATATATGATGAAATTTATCCCGTAATTAGTTTA
TACAATCTAGTTAGAAATTATGTTACACAAAAGCCGTATAGTACCAAGAAAATAAAGCTT
AATTTCGGAATACCTACGCTTGCTGATGGTTGGTCAAAAAGTAAAGAATATAGCAATAAT
GCAATAATTTTAATGAGAGATAACCTATATTATTTGGGTATTTTTAACGCTAAGAACAAA
CCAGACAAGAAAATAATTGAAGGTAATACATCTGAAAACAAGGGCGACTATAAAAAGATG
ATATACAATTTGCTCCCAGGTCCTAATAAAATGATTCCTAAGGTTTTCCTGAGTAGCAAG
ACTGGCGTTGAAACTTACAAGCCTAGTGCGTATATCCTGGAGGGTTATAAACAGAACAAG
CATATCAAATCCTCTAAGGACTTCGATATCACCTTTTGCCATGACTTAATCGATTATTTT
AAAAATTGTATCGCAATTCATCCAGAATGGAAAAATTTCGGATTTGATTTTAGTGATACC
AGCACTTACGAGGATATCTCTGGGTTCTACAGAGAAGTGGAGTTGCAGGGCTACAAAATC
GATTGGACTTACATATCTGAAAAGGACATAGATTTGCTGCAGGAGAAAGGTCAGCTATAT
TTGTTTCAAATCTACAACAAAGACTTTTCTAAAAAGTCTACCGGTAATGACAATCTGCAC
ACAATGTACTTGAAGAACTTATTCTCCGAGGAGAACTTAAAGGACATTGTACTCAAGTTG
AATGGAGAAGCCGAGATTTTTTTTAGAAAGAGCAGTATAAAGAATCCTATAATCCACAAG
AAGGGCTCAATTCTCGTGAATAGGACGTATGAGGCAGAAGAAAAGGACCAATTTGGGAAT
ATACAAATTGTAAGAAAAAACATCCCAGAAAATATCTACCAGGAATTATATAAGTATTTT
AATGACAAATCTGATAAGGAACTGTCTGACGAAGCCGCTAAGCTCAAGAATGTTGTGGGC
CACCATGAAGCTGCTACTAATATAGTGAAGGACTACAGATATACCTACGATAAATATTTC
CTGCATATGCCAATTACTATAAACTTCAAAGCAAATAAAACAGGTTTTATAAATGATAGA
ATCCTGCAGTATATTGCTAAAGAAAAGGATTTACATGTAATTGGGATTGATAGAGGTGAA
CGCAATCTGATCTATGTCAGCGTAATAGATACTTGTGGTAATATTGTGGAACAAAAGTCC
TTTAATATTGTGAACGGATATGATTACCAAATCAAGTTGAAACAACAAGAGGGAGCACGC
CAAATTGCCCGTAAGGAATGGAAAGAGATAGGTAAGATCAAGGAAATTAAGGAAGGTTAT
CTTTCATTAGTTATTCACGAAATTTCGAAGATGGTAATCAAATACAACGCAATAATTGCT
ATGGAGGACCTGTCATATGGATTTAAGAAAGGTAGATTCAAGGTTGAGAGACAGGTATAC
CAGAAATTTGAAACTATGTTGATCAACAAATTAAATTACTTAGTCTTTAAGGACATATCA
ATAACGGAAAACGGCGGGCTTTTAAAAGGGTATCAACTTACATACATACCTGATAAGTTG
AAAAATGTGGGTCATCAGTGTGGGTGCATCTTTTATGTTCCAGCCGCTTACACATCAAAA
ATCGATCCTACTACTGGGTTCGTAAACATATTTAAATTTAAAGATCTAACCGTTGATGCA
AAAAGAGAGTTTATCAAGAAATTTGATAGCATTAGGTACGATTCAGAAAAAAATCTATTC
TGTTTTACTTTTGACTACAACAACTTTATAACGCAGAATACAGTGATGTCAAAATCGTCC
TGGTCAGTGTATACTTATGGTGTTAGAATTAAGAGACGTTTCGTAAACGGTCGTTTTTCT
AACGAGTCCGATACAATCGACATCACTAAAGATATGGAAAAAACTTTGGAAATGACAGAT
ATAAACTGGAGAGATGGTCACGACCTTAGACAAGATATAATCGATTATGAAATCGTACAG
CATATTTTTGAAATTTTTCGCTTAACAGTTCAGATGCGTAACTCTCTTAGTGAGCTAGAA
GATAGAGATTATGATAGACTTATCTCGCCTGTTCTTAACGAAAATAATATCTTCTATGAC
TCGGCAAAAGCCGGTGATGCACTTCCAAAAGATGCTGATGCAAATGGCGCGTACTGCATC
GCATTGAAGGGGCTCTACGAGATTAAACAAATCACCGAAAACTGGAAAGAAGATGGTAAA
TTTTCTAGGGATAAGTTGAAAATCAGTAATAAAGATTGGTTCGATTTTATACAAAATAAG
CGATACTTATAG
SEQ ATGACCAATAAGTTTACTAATCAATACTCATTGTCTAAAACGTTAAGATTCGAGTTAATT
ID CCCCAGGGAAAGACACTAGAATTTATTCAAGAAAAAGGTCTTCTCTCTCAGGATAAACAA
NO: AGAGCAGAATCATACCAGGAGATGAAAAAAACCATAGATAAATTTCATAAGTACTTCATC
134 GACTTGGCACTATCGAACGCCAAGCTAACACATTTGGAAACCTACCTGGAGTTGTATAAT
AAATCGGCAGAGACGAAAAAGGAACAAAAATTCAAGGATGACCTGAAGAAGGTTCAAGAT
AATCTGCGAAAGGAAATAGTGAAGTCGTTTAGTGATGGTGATGCAAAGTCAATCTTTGCT
ATTTTAGACAAGAAGGAATTAATAACCGTGGAACTTGAAAAGTGGTTTGAAAATAACGAA
CAGAAAGATATTTACTTCGACGAAAAATTTAAAACGTTTACTACGTACTTTACAGGGTTC
CATCAGAACCGCAAAAACATGTACTCCGTTGAACCAAACTCTACTGCAATCGCCTACAGA
TTAATACACGAAAATTTGCCTAAGTTTTTAGAAAATGCAAAGGCTTTTGAAAAGATAAAG
CAAGTCGAATCGTTACAGGTAAACTTTCGCGAATTAATGGGCGAATTTGGAGATGAAGGT
CTTATTTTTGTCAATGAATTAGAGGAAATGTTTCAAATTAATTATTATAACGATGTCTTG
AGTCAGAACGGCATTACTATCTACAACTCAATTATCAGTGGTTTCACTAAGAATGATATA
AAATATAAAGGTTTGAATGAATACATTAATAATTATAATCAAACTAAAGATAAGAAGGAC
AGGCTTCCGAAATTGAAGCAATTGTACAAGCAGATTCTAAGTGATAGGATTAGTTTGTCT
TTCTTGCCAGACGCATTTACTGATGGCAAGCAAGTCTTAAAGGCTATATTCGATTTCTAC
AAGATTAACCTACTTTCGTACACAATTGAAGGTCAAGAAGAATCTCAAAATCTGCTGCTT
TTGATTAGGCAAACTATAGAAAATTTGTCGTCCTTTGACACTCAAAAAATTTACCTGAAG
AATGATACACACCTGACTACAATATCACAGCAGGTCTTTGGGGATTTTTCTGTCTTCTCC
ACGGCCCTAAACTATTGGTATGAGACAAAAGTTAATCCAAAATTTGAAACAGAATATAGT
AAGGCGAATGAAAAAAAGAGAGAAATTTTGGATAAAGCGAAGGCAGTATTCACAAAACAA
GACTATTTTTCTATCGCATTTCTCCAAGAAGTCTTATCCGAATATATTTTGACACTCGAT
CACACCTCTGATATAGTTAAGAAACATTCGTCCAACTGCATCGCAGATTACTTCAAGAAT
CACTTCGTGGCTAAGAAAGAAAACGAAACGGATAAAACTTTTGACTTCATTGCTAACATA
ACCGCTAAATACCAATGTATTCAGGGCATATTAGAAAATGCAGACCAGTACGAAGACGAG
TTAAAACAGGACCAAAAGTTAATAGATAATCTAAAGTTTTTCTTAGATGCTATACTTGAG
TTATTACATTTTATAAAGCCATTGCATCTAAAATCGGAAAGTATTACTGAAAAAGACACT
GCGTTCTATGATGTGTTCGAAAATTATTATGAGGCTTTATCTTTATTGACCCCCCTTTAC
AACATGGTCCGCAATTATGTTACTCAGAAGCCTTACTCTACTGAAAAGATCAAATTAAAC
TTTGAAAATGCTCAGTTGCTGAATGGTTGGGATGCCAATAAGGAAGGTGACTACCTGACG
ACTATTCTAAAAAAAGACGGTAATTATTTCTTAGCAATCATGGATAAAAAACATAACAAG
GCATTTCAAAAATTTCCAGAAGGAAAAGAAAACTATGAAAAGATGGTTTATAAATTGTTG
CCTGGAGTTAATAAAATGTTGCCAAAAGTTTTTTTTAGCAATAAGAACATAGCTTACTTT
AATCCATCTAAGGAACTGCTCGAGAACTACAAGAAGGAAACACATAAAAAAGGTGATACA
TTTAATTTGGAACATTGCCATACTCTGATTGATTTTTTTAAGGACTCTCTTAATAAACAT
GAAGACTGGAAATATTTTGATTTTCAATTTTCGGAAACTAAATCATACCAAGATCTAAGT
GGATTTTACAGAGAAGTTGAACACCAAGGTTATAAGATTAACTTCAAGAATATAGATTCT
GAATACATTGATGGTCTTGTAAACGAGGGTAAACTATTCCTGTTCCAAATCTACTCTAAG
GACTTCTCACCTTTTTCCAAAGGAAAACCTAATATGCATACGTTGTACTGGAAGGCTCTA
TTTGAAGAACAAAATTTGCAAAATGTAATCTACAAACTGAACGGCCAAGCTGAAATATTC
TTCAGAAAAGCCTCAATTAAGCCAAAAAACATTATTCTTCATAAAAAGAAGATCAAGATT
GCGAAGAAACATTTTATTGATAAGAAGACCAAGACTTCCGAAATTGTACCAGTACAAACA
ATCAAGAATCTCAATATGTATTATCAAGGCAAGATAAGTGAGAAAGAGTTAACCCAGGAT
GATTTACGTTATATAGACAATTTCTCTATATTCAACGAGAAGAACAAAACAATAGACATT
ATCAAAGATAAAAGGTTTACTGTTGACAAATTTCAATTTCATGTGCCTATCACAATGAAC
TTTAAGGCCACAGGTGGTTCGTACATTAATCAAACTGTTTTAGAATATCTGCAAAATAAC
CCAGAGGTCAAGATCATCGGTCTTGATAGGGGTGAGAGACATCTGGTGTATCTAACACTC
ATTGATCAACAAGGCAACATCTTGAAGCAAGAATCATTGAACACTATCACAGACTCCAAG
ATCTCGACTCCATATCACAAACTCCTTGACAATAAAGAAAACGAAAGGGATCTTGCCAGA
AAAAATTGGGGTACAGTTGAAAATATTAAGGAACTAAAAGAAGGTTACATTTCGCAAGTA
GTTCACAAGATTGCAACACTCATGTTGGAAGAAAACGCAATCGTTGTCATGGAAGATTTA
AATTTCGGATTTAAGAGAGGAAGATTTAAAGTAGAAAAGCAAATCTACCAGAAGTTGGAG
AAGATGTTAATTGACAAATTGAACTACTTAGTGCTGAAAGACAAACAGCCTCAAGAATTG
GGCGGTCTATACAACGCTTTACAACTGACAAATAAATTTGAGTCATTCCAAAAGATGGGT
AAGCAGAGTGGTTTTTTGTTTTATGTTCCGGCATGGAACACATCCAAAATCGATCCAACT
ACAGGCTTCGTGAATTATTTCTACACTAAATATGAAAATGTGGATAAAGCAAAAGCTTTC
TTTGAGAAGTTCGAGGCGATCCGTTTTAACGCTGAAAAGAAGTACTTCGAGTTCGAGGTC
AAAAAGTATTCAGATTTTAACCCCAAGGCTGAAGGCACCCAGCAAGCATGGACTATTTGC
ACGTACGGTGAGCGAATCGAAACTAAAAGGCAAAAGGATCAAAATAATAAGTTTGTAAGC
ACACCCATTAACTTGACAGAAAAGATAGAAGATTTTCTTGGAAAAAACCAAATTGTATAT
GGTGACGGTAACTGTATCAAGTCACAAATTGCTTCTAAAGACGATAAGGCCTTCTTCGAA
ACTCTGCTATACTGGTTTAAAATGACGTTGCAAATGAGAAACAGTGAAACTAGAACTGAT
ATCGACTATTTAATATCACCCGTGATGAACGATAATGGTACCTTTTACAATTCAAGAGAT
TACGAGAAATTGGAGAACCCCACACTACCAAAAGACGCAGACGCTAATGGTGCCTACCAT
ATTGCTAAAAAGGGACTGATGTTGTTGAACAAGATAGATCAAGCCGACTTAACTAAAAAA
GTTGATTTGTCAATTTCGAATAGAGATTGGTTGCAATTCGTCCAGAAAAATAAGTAA
SEQ ATGGAACAGGAATACTACTTGGGTTTGGATATGGGAACTGGTTCAGTCGGTTGGGCTGTT
ID ACGGACTCCGAGTACCACGTGTTGAGAAAACACGGAAAGGCTTTATGGGGTGTCAGACTA
NO: TTCGAATCAGCATCGACCGCGGAAGAGAGAAGAATGTTTAGAACTTCAAGAAGAAGGCTG
135 GATCGTAGGAATTGGCGGATAGAAATTTTACAAGAAATATTCGCCGAAGAAATCTCTAAA
AAAGATCCAGGATTTTTTCTACGTATGAAGGAATCCAAATACTATCCGGAAGATAAACGT
GATATTAATGGCAATTGTCCAGAGTTACCCTATGCTTTATTTGTGGACGACGATTTCACC
GATAAAGATTACCATAAGAAGTTCCCAACAATTTACCATCTGAGAAAGATGTTAATGAAC
ACTGAAGAAACCCCGGATATAAGACTGGTCTATCTAGCCATTCATCATATGATGAAACAC
AGGGGACACTTCTTGCTATCAGGGGATATAAATGAAATTAAAGAATTTGGTACAACATTT
TCTAAATTATTGGAAAATATTAAAAACGAAGAATTAGATTGGAATTTAGAATTAGGCAAG
GAGGAATACGCAGTTGTCGAATCGATTCTGAAAGATAACATGTTGAACAGATCAACGAAA
AAAACAAGGCTGATCAAGGCTTTAAAAGCGAAATCAATATGCGAAAAAGCAGTATTGAAT
TTGTTAGCTGGGGGGACTGTCAAGTTGTCTGATATTTTCGGATTGGAAGAATTGAATGAA
ACAGAGAGACCGAAGATATCCTTCGCCGATAATGGCTACGATGATTATATAGGCGAAGTC
GAAAATGAGCTGGGCGAACAATTCTACATTATCGAGACTGCCAAGGCTGTTTATGATTGG
GCGGTGTTAGTCGAAATCCTTGGCAAATACACTTCCATCTCCGAAGCTAAGGTGGCAACC
TACGAAAAGCATAAAAGTGATTTGCAATTCCTTAAGAAAATTGTCCGAAAGTACTTGACC
AAAGAAGAGTACAAGGATATTTTCGTATCAACATCGGACAAACTGAAGAATTATTCAGCT
TATATTGGCATGACGAAAATTAATGGTAAGAAAGTTGATTTGCAATCCAAGAGATGTTCT
AAAGAAGAATTTTACGATTTCATTAAAAAAAATGTCCTAAAAAAGTTGGAGGGACAACCT
GAATATGAGTATTTAAAGGAAGAACTGGAAAGAGAAACTTTCCTACCAAAGCAAGTTAAT
CGTGATAATGGCGTTATTCCATACCAAATACACTTGTACGAATTAAAGAAGATCTTGGGT
AACTTGAGGGACAAAATTGATTTAATCAAGGAAAATGAAGACAAACTGGTACAATTATTT
GAATTTAGAATACCTTACTACGTGGGCCCTTTAAACAAAATAGACGATGGTAAGGAAGGG
AAGTTCACATGGGCAGTCAGAAAGTCCAATGAAAAAATTTACCCATGGAATTTCGAAAAC
GTTGTAGATATTGAAGCTTCTGCTGAGAAATTTATTAGGAGAATGACAAATAAATGCACT
TATCTTATGGGGGAAGACGTGTTGCCTAAAGATAGTTTATTATATTCAAAGTATATGGTC
TTAAATGAATTAAACAATGTTAAATTAGATGGTGAAAAACTTTCCGTCGAATTGAAACAA
AGATTGTATACAGATGTATTCTGCAAATATAGAAAAGTAACTGTAAAGAAGATTAAAAAC
TACCTTAAATGTGAAGGCATTATCAGCGGAAATGTTGAGATCACTGGTATCGATGGTGAT
TTTAAGGCATCTTTAACCGCATATCACGACTTTAAGGAAATATTGACGGGTACTGAGCTT
GCTAAAAAAGACAAAGAGAACATTATCACCAATATCGTGCTCTTCGGAGACGACAAGAAA
TTATTGAAAAAGAGATTGAACCGCCTATACCCTCAGATTACCCCTAACCAATTGAAGAAA
ATCTGCGCTCTGTCTTATACTGGATGGGGTCGTTTTAGCAAGAAGTTTCTAGAAGAAATT
ACTGCTCCGGATCCTGAAACTGGGGAAGTCTGGAATATAATTACCGCGCTATGGGAATCG
AATAATAATTTAATGCAATTACTATCTAATGAATACAGATTTATGGAAGAAGTCGAAACT
TACAATATGGGAAAACAAACAAAAACTTTGAGCTACGAAACAGTAGAGAATATGTATGTC
TCACCATCTGTAAAGCGGCAGATCTGGCAAACCTTGAAGATAGTTAAAGAATTAGAAAAA
GTGATGAAGGAAAGTCCAAAAAGGGTTTTTATTGAAATGGCCCGAGAAAAACAAGAATCT
AAAAGGACGGAAAGTAGGAAAAAGCAACTTATAGATCTATATAAAGCCTGCAAAAATGAA
GAAAAAGATTGGGTAAAGGAATTAGGTGACCAGGAAGAGCAAAAATTGAGATCTGACAAG
CTGTACTTGTATTATACGCAAAAGGGCCGGTGTATGTATTCGGGTGAGGTAATAGAATTG
AAAGATTTATGGGATAACACTAAGTATGACATTGACCATATTTACCCCCAGTCTAAGACA
ATGGACGATTCATTAAATAACCGAGTTCTTGTCAAAAAGAAGTACAATGCCACAAAGAGC
GATAAGTACCCATTGAACGAAAATATAAGACATGAACGAAAAGGTTTCTGGAAATCATTG
TTGGACGGTGGATTTATTTCCAAAGAAAAATACGAGAGATTGATTAGAAACACTGAACTA
TCTCCAGAGGAGTTAGCTGGCTTTATCGAAAGACAAATTGTTGAAACTAGACAGTCTACA
AAAGCAGTTGCAGAAATCTTAAAACAAGTATTTCCAGAATCCGAAATTGTGTACGTCAAA
GCCGGAACAGTAAGTAGATTTAGAAAAGACTTTGAATTATTGAAAGTACGAGAGGTTAAC
GACCTACATCATGCTAAGGATGCTTATTTAAATATAGTCGTTGGTAATTCGTATTACGTG
AAATTCACAAAAAACGCATCTTGGTTCATCAAGGAGAATCCTGGTAGGACATACAACTTG
AAAAAGATGTTTACATCAGGATGGAATATCGAAAGAAATGGTGAGGTTGCGTGGGAGGTA
GGCAAGAAGGGAACCATTGTTACTGTAAAGCAAATTATGAATAAAAACAATATACTTGTT
ACGAGACAGGTGCACGAAGCCAAAGGAGGGTTGTTTGACCAGCAAATCATGAAGAAAGGT
AAAGGTCAGATAGCAATAAAAGAGACTGATGAGCGTTTAGCTAGTATAGAAAAATATGGG
GGCTACAATAAGGCAGCTGGTGCTTACTTCATGTTGGTCGAATCAAAGGATAAAAAAGGG
AAGACGATCCGGACCATAGAGTTTATCCCTCTGTACTTGAAGAATAAGATTGAGTCTGAC
GAAAGCATCGCATTGAATTTCTTGGAAAAGGGGCGCGGTCTAAAGGAGCCAAAAATATTG
TTAAAGAAAATTAAAATAGACACCCTATTCGACGTCGATGGGTTTAAGATGTGGCTTAGT
GGTCGTACTGGGGACAGATTATTATTCAAGTGTGCCAATCAGTTAATCCTTGACGAGAAA
ATCATTGTTACAATGAAAAAAATTGTTAAGTTTATTCAAAGGCGACAAGAAAATAGAGAA
CTAAAGTTGAGTGATAAGGATGGAATCGATAATGAAGTGTTAATGGAGATTTATAACACT
TTTGTCGACAAATTGGAGAATACGGTGTACAGAATTAGGCTATCTGAACAGGCTAAAACC
CTAATTGATAAACAGAAGGAGTTTGAGCGACTTTCTCTTGAAGACAAATCTTCAACTCTT
TTCGAGATCCTACATATCTTTCAGTGTCAATCTTCTGCAGCTAATTTGAAAATGATTGGA
GGTCCTGGTAAGGCTGGTATATTAGTCATGAACAACAACATATCTAAGTGTAATAAGATT
AGTATAATTAACCAATCACCGACAGGTATCTTTGAAAATGAAATTGATTTACTTAAA
SEQ ATGAAATCATTCGACTCGTTCACCAACTTGTACTCCCTGTCTAAAACATTGAAATTTGAA
ID ATGCGACCTGTTGGTAACACCCAAAAGATGTTAGATAATGCAGGAGTTTTCGAAAAGGAT
NO: AAACTGATCCAGAAAAAATACGGTAAAACGAAACCATATTTCGATAGGTTGCATCGGGAA
136 TTTATAGAAGAAGCTTTGACTGGTGTAGAATTAATTGGCTTAGATGAGAATTTCCGTACT
CTAGTCGATTGGCAAAAAGATAAAAAGAACAATGTTGCCATGAAGGCATACGAAAATAGT
CTACAAAGACTAAGAACAGAGATCGGGAAAATTTTCAATTTGAAGGCAGAAGACTGGGTG
AAGAACAAATATCCAATATTGGGTCTTAAGAATAAGAATACTGATATATTGTTCGAGGAG
GCCGTTTTCGGTATTCTTAAGGCAAGATATGGTGAAGAGAAAGACACGTTTATTGAAGTT
GAGGAGATTGATAAAACCGGTAAGTCCAAAATCAACCAGATCTCTATCTTCGACAGTTGG
AAGGGCTTCACTGGTTATTTTAAGAAGTTCTTCGAAACTAGGAAGAACTTCTATAAAAAC
GATGGTACTTCCACGGCTATTGCTACAAGAATTATCGACCAAAACCTTAAGCGTTTTATT
GATAACCTATCAATTGTTGAAAGTGTTCGACAGAAAGTAGATTTGGCTGAAACTGAAAAA
TCTTTTAGTATCTCCTTATCCCAGTTTTTCTCTATAGATTTTTATAATAAATGTTTGCTG
CAAGATGGCATTGACTACTATAATAAAATAATTGGTGGAGAGACATTGAAAAACGGAGAG
AAGCTGATTGGCCTTAATGAGTTGATAAATCAATATAGACAAAATAATAAGGACCAGAAA
ATCCCTTTCTTTAAATTGCTAGACAAACAGATTTTGTCTGAAAAGATCCTATTCTTGGAT
GAAATAAAGAACGATACTGAATTGATTGAAGCTTTGTCCCAGTTTGCTAAAACAGCTGAA
GAAAAGACAAAGATTGTGAAAAAATTGTTTGCTGATTTCGTAGAAAACAATTCTAAATAT
GATCTAGCCCAGATTTATATAAGTCAAGAAGCTTTCAATACAATAAGTAATAAGTGGACA
AGTGAAACAGAAACTTTTGCTAAGTATTTATTCGAAGCCATGAAGTCTGGTAAACTTGCC
AAATACGAAAAAAAAGATAACAGTTATAAATTTCCAGACTTTATAGCCCTTTCACAGATG
AAGTCTGCCTTATTGTCGATATCCTTAGAAGGTCATTTTTGGAAGGAAAAATATTATAAG
ATAAGCAAGTTCCAAGAAAAGACTAATTGGGAACAATTTTTGGCTATATTTCTATATGAG
TTCAATTCATTATTTTCCGATAAAATCAACACTAAGGATGGAGAGACTAAGCAAGTTGGC
TACTATTTGTTCGCAAAAGATCTGCACAATTTGATTCTATCAGAACAAATAGATATACCA
AAAGATTCAAAGGTAACTATAAAGGATTTCGCAGATTCCGTCCTCACCATTTATCAAATG
GCTAAATATTTTGCCGTTGAAAAAAAGAGAGCGTGGTTAGCAGAATACGAGTTGGACTCG
TTTTATACTCAGCCAGATACTGGATACTTGCAATTCTACGATAATGCATACGAAGACATT
GTACAGGTATACAATAAACTTAGAAATTACTTAACCAAGAAGCCCTACAGTGAAGAAAAA
TGGAAGCTGAACTTTGAAAATTCGACTTTGGCAAATGGTTGGGATAAAAATAAAGAAAGT
GACAACTCCGCAGTGATTTTGCAAAAGGGTGGGAAATATTACTTGGGTTTAATCACAAAA
GGCCACAATAAGATTTTTGATGATAGATTTCAAGAAAAATTCATAGTTGGTATAGAAGGT
GGCAAATACGAGAAAATTGTCTATAAATTCTTCCCTGATCAAGCCAAAATGTTCCCAAAA
GTTTGCTTTTCTGCTAAAGGATTGGAGTTTTTCCGGCCTAGCGAGGAGATCCTTCGTATC
TACAACAATGCTGAATTCAAAAAAGGAGAAACCTATAGCATAGATTCTATGCAAAAACTG
ATAGATTTTTATAAGGATTGTTTAACAAAGTACGAAGGCTGGGCCTGCTATACATTTAGA
CATTTAAAGCCCACAGAAGAATACCAAAATAACATTGGTGAATTCTTTCGGGACGTTGCC
GAAGACGGCTATAGGATCGATTTTCAAGGTATCTCAGATCAATATATCCACGAAAAGAAC
GAGAAGGGTGAGCTGCACCTTTTCGAAATTCATAATAAGGACTGGAATTTGGATAAGGCG
AGAGATGGTAAATCGAAGACCACTCAAAAGAACTTGCATACTTTATATTTTGAGTCCTTG
TTTTCTAATGATAACGTCGTCCAAAATTTTCCAATAAAGTTGAATGGACAAGCGGAAATT
TTCTATCGGCCTAAGACAGAGAAAGACAAATTAGAATCAAAGAAAGATAAAAAGGGAAAT
AAAGTCATTGATCACAAACGATACTCTGAGAATAAAATATTTTTCCACGTACCATTGACA
CTCAACAGGACTAAGAATGACTCTTATAGATTTAATGCTCAGATTAATAATTTTTTGGCA
AATAACAAGGATATTAACATAATTGGGGTGGATAGAGGTGAAAAGCACTTGGTATATTAC
TCTGTCATCACTCAGGCTTCTGATATATTGGAAAGCGGGTCTCTAAATGAATTGAACGGT
GTTAACTACGCCGAAAAGCTAGGTAAAAAAGCTGAAAACAGAGAGCAGGCTCGGCGCGAT
TGGCAAGATGTTCAAGGAATTAAAGACCTTAAAAAAGGCTACATTAGTCAAGTAGTTAGA
AAGTTAGCCGATCTTGCTATTAAACATAACGCAATCATTATTCTGGAGGACCTAAATATG
CGTTTTAAGCAAGTTAGGGGTGGCATAGAAAAAAGTATTTATCAGCAGCTTGAGAAGGCT
TTGATAGATAAGTTATCGTTCCTAGTTGACAAAGGTGAAAAAAATCCTGAACAAGCTGGT
CATCTGTTGAAAGCTTATCAGCTGAGCGCACCTTTTGAAACATTTCAAAAAATGGGAAAA
CAAACAGGTATTATTTTCTATACTCAAGCGAGTTATACAAGTAAATCTGACCCAGTGACA
GGATGGAGACCACACCTTTATCTAAAATATTTTTCTGCTAAAAAGGCCAAAGATGACATC
GCTAAGTTTACAAAAATAGAATTTGTCAACGATAGATTTGAATTGACTTACGATATTAAA
GATTTTCAGCAAGCAAAAGAATACCCAAATAAGACAGTGTGGAAAGTATGCTCCAATGTG
GAGAGATTTAGATGGGATAAAAATCTCAATCAAAACAAGGGTGGTTACACACATTATACT
AATATAACTGAAAATATTCAAGAATTGTTTACTAAGTACGGAATTGACATAACCAAAGAC
TTACTAACTCAGATTTCAACTATTGACGAAAAACAAAATACCTCATTTTTCCGCGACTTT
ATTTTTTATTTCAACTTGATCTGTCAAATTCGTAACACGGATGATTCCGAAATTGCCAAG
AAGAACGGAAAAGATGATTTCATCCTATCTCCAGTGGAACCATTTTTTGACTCAAGAAAA
GATAATGGTAATAAGTTGCCTGAGAACGGAGATGATAACGGCGCTTATAATATCGCTCGG
AAGGGTATTGTAATTCTTAATAAAATATCTCAGTACTCTGAAAAGAACGAAAACTGCGAG
AAAATGAAGTGGGGCGACTTGTATGTATCTAATATAGATTGGGATAATTTCGTTACTCAA
GCCAACGCGAGACATTGA
SEQ ATGGAAAATTTTAAAAACCTATATCCAATTAATAAGACACTTAGATTCGAGCTTAGGCCA
ID TACGGCAAAACACTAGAAAATTTTAAGAAGTCAGGCCTATTAGAAAAAGACGCCTTTAAG
NO: GCAAATTCCAGAAGATCAATGCAGGCAATTATTGATGAGAAATTTAAAGAGACTATCGAG
137 GAAAGGTTGAAATACACTGAATTCTCTGAGTGCGATCTGGGAAACATGACTTCCAAGGAT
AAAAAGATTACCGATAAGGCTGCTACCAACCTCAAAAAGCAAGTCATCTTATCGTTTGAT
GATGAAATTTTTAATAACTACTTAAAGCCGGACAAAAACATTGACGCCCTATTCAAAAAT
GATCCGTCCAACCCCGTAATTTCAACTTTTAAGGGTTTTACCACGTACTTTGTAAATTTT
TTTGAGATTCGTAAACATATCTTCAAAGGAGAATCGTCGGGTTCCATGGCCTATAGGATA
ATTGATGAAAATCTTACGACTTACTTAAACAATATCGAAAAGATAAAAAAGTTACCAGAA
GAATTAAAGTCTCAATTGGAAGGTATTGACCAAATAGACAAATTAAATAACTATAATGAG
TTCATAACTCAAAGCGGTATCACACATTACAATGAAATTATCGGTGGTATATCTAAAAGT
GAGAACGTAAAAATACAGGGAATAAACGAGGGGATCAATCTATACTGTCAGAAGAATAAA
GTAAAATTACCAAGACTAACGCCATTATACAAAATGATTCTGTCTGATAGAGTTTCCAAC
TCGTTCGTGCTTGATACTATAGAAAATGATACTGAATTAATTGAGATGATTAGCGACTTG
ATTAATAAAACAGAAATATCTCAAGACGTAATAATGTCAGACATTCAGAACATTTTCATA
AAATATAAACAGCTTGGTAATTTACCGGGGATAAGTTACTCTAGCATCGTGAATGCTATT
TGCTCCGATTATGACAATAATTTTGGTGACGGAAAAAGAAAAAAATCATATGAGAACGAT
AGGAAGAAACACCTTGAAACAAACGTATACTCAATTAACTATATATCGGAACTGTTAACA
GACACCGATGTATCATCTAATATAAAAATGAGATATAAGGAACTTGAACAAAATTACCAG
GTGTGTAAGGAGAATTTCAATGCTACCAACTGGATGAACATTAAGAATATTAAACAGAGT
GAAAAGACAAACTTGATTAAAGATCTACTAGATATACTGAAATCAATACAGAGATTCTAC
GATCTGTTTGATATAGTTGATGAAGACAAAAATCCTAGTGCTGAGTTTTACACGTGGCTA
AGTAAAAATGCGGAAAAGTTAGATTTCGAGTTCAACTCTGTTTATAATAAATCTAGGAAT
TATTTAACTAGAAAGCAGTATTCTGATAAAAAGATAAAATTGAACTTCGACTCCCCTACG
TTGGCAAAGGGTTGGGATGCAAACAAAGAAATCGATAACTCCACCATAATAATGCGTAAG
TTTAACAATGATAGGGGGGATTACGATTATTTTTTGGGAATTTGGAACAAATCTACCCCA
GCGAATGAAAAAATTATTCCCCTTGAAGACAATGGTCTTTTTGAAAAAATGCAGTATAAA
TTATATCCAGACCCATCCAAGATGCTTCCAAAGCAATTTCTGTCAAAAATTTGGAAGGCT
AAACACCCTACTACTCCTGAATTTGATAAGAAGTATAAGGAGGGCCGACACAAAAAGGGT
CCAGATTTTGAAAAAGAATTCCTGCATGAATTGATAGATTGTTTTAAGCATGGTTTGGTA
AATCATGATGAAAAATATCAGGATGTCTTTGGATTCAATTTGAGAAATACAGAGGATTAC
AACTCATATACAGAATTTCTCGAGGACGTCGAACGTTGCAATTATAATCTCAGTTTCAAC
AAGATCGCAGACACTTCAAACTTAATTAACGACGGAAAATTGTACGTTTTTCAAATCTGG
TCGAAAGACTTTAGTATTGATTCAAAGGGTACAAAAAACCTAAATACAATATATTTCGAA
AGTCTATTCTCGGAAGAAAACATGATCGAAAAAATGTTCAAACTGTCAGGCGAAGCTGAA
ATATTCTACCGTCCCGCAAGCCTTAATTATTGTGAGGATATCATTAAAAAAGGACATCAC
CATGCAGAGTTAAAAGATAAATTCGATTACCCAATAATTAAAGATAAAAGATACTCCCAG
GATAAGTTCTTTTTCCATGTACCTATGGTTATTAACTACAAGTCGGAAAAACTAAACTCG
AAGTCATTAAATAATAGAACTAACGAGAACTTGGGACAATTCACACATATAATTGGTATT
GATCGTGGCGAAAGACATTTAATATATCTGACTGTTGTTGATGTTTCAACAGGAGAAATT
GTTGAACAGAAACATCTTGATGAAATTATAAACACAGATACAAAAGGCGTTGAGCATAAA
ACTCATTATCTAAATAAATTGGAGGAAAAGTCGAAGACTCGCGATAACGAGAGAAAGAGT
TGGGAAGCAATTGAAACCATAAAAGAGCTTAAAGAAGGTTACATTAGTCACGTCATCAAT
GAAATACAAAAGTTACAAGAAAAGTATAACGCTTTGATTGTAATGGAAAATCTAAATTAT
GGTTTTAAGAATTCAAGAATCAAAGTCGAAAAGCAGGTCTATCAGAAATTTGAAACGGCA
CTTATTAAAAAGTTTAACTACATTATTGATAAAAAGGACCCAGAAACTTATATTCATGGT
TACCAACTGACGAACCCAATCACAACATTGGACAAAATTGGAAACCAAAGTGGAATTGTT
TTATACATTCCAGCTTGGAATACATCCAAAATAGACCCTGTCACGGGGTTTGTCAACTTG
TTATATGCCGACGATTTAAAGTATAAAAACCAAGAACAAGCAAAGTCTTTTATTCAAAAG
ATTGATAATATTTATTTCGAAAACGGTGAATTTAAATTCGACATAGATTTTTCTAAATGG
AACAACCGTTATTCAATAAGTAAAACTAAATGGACACTCACCTCATACGGCACTCGTATC
CAAACCTTTCGGAATCCCCAAAAAAATAACAAATGGGATTCTGCAGAATACGACTTGACC
GAGGAATTTAAATTAATTCTTAATATAGACGGTACACTCAAAAGTCAAGACGTGGAGACA
TACAAGAAGTTTATGTCGTTATTCAAGCTTATGCTTCAGTTGAGGAACTCCGTTACAGGC
ACTGATATTGATTACATGATTTCACCAGTAACGGATAAGACTGGGACTCATTTCGATTCT
AGGGAAAATATTAAAAATTTACCTGCTGACGCAGACGCAAACGGCGCATACAATATAGCA
AGAAAAGGGATTATGGCCATTGAGAATATTATGAATGGCATATCAGATCCATTAAAGATA
AGCAATGAAGACTACTTAAAATACATTCAGAATCAGCAAGAATAA
SEQ ATGACCCAGTTTGAAGGTTTCACCAATTTGTACCAAGTAAGTAAAACCTTGAGGTTCGAA
ID TTGATCCCACAGGGCAAGACATTGAAGCATATTCAAGAGCAAGGATTTATAGAAGAAGAT
NO: AAAGCGAGAAACGATCACTATAAAGAGTTAAAACCCATTATTGACAGGATCTATAAAACA
138 TACGCCGATCAATGCCTTCAATTAGTGCAATTAGATTGGGAAAACTTGAGCGCTGCCATC
GATTCCTACAGGAAGGAAAAAACAGAAGAAACAAGAAATGCCTTAATCGAGGAACAAGCA
ACCTATAGAAACGCTATACACGATTACTTCATCGGTAGAACTGATAATCTAACAGATGCA
ATAAATAAGAGACATGCTGAGATATATAAAGGACTATTTAAAGCAGAATTATTCAACGGA
AAGGTGTTGAAACAGTTAGGTACCGTTACAACTACTGAGCATGAAAATGCCTTGCTGAGA
AGCTTTGACAAGTTTACTACCTACTTTTCGGGTTTCTACGAAAATCGCAAAAATGTATTT
TCTGCGGAAGATATTTCAACTGCAATCCCTCATAGGATTGTTCAAGATAATTTCCCTAAG
TTTAAAGAGAACTGTCACATTTTTACAAGGTTAATTACTGCGGTTCCAAGTCTAAGAGAA
CATTTTGAGAATGTAAAAAAAGCGATTGGTATATTTGTATCCACTAGCATTGAAGAGGTT
TTCAGCTTCCCTTTTTATAACCAATTACTTACCCAAACACAGATCGACCTGTACAACCAA
TTGTTAGGTGGTATATCGAGGGAGGCTGGTACGGAAAAGATTAAAGGATTAAATGAAGTT
CTTAATTTGGCCATACAAAAAAATGATGAAACCGCGCACATTATCGCATCTTTACCACAT
AGGTTTATACCGTTATTCAAGCAAATATTATCTGATCGTAATACCTTATCGTTCATATTA
GAGGAGTTTAAATCTGACGAAGAAGTTATACAATCTTTTTGCAAGTATAAGACGCTATTG
AGAAACGAAAACGTTCTGGAAACAGCCGAAGCACTGTTCAATGAATTAAACAGTATCGAC
TTGACTCATATTTTTATATCGCATAAAAAGTTGGAGACAATTTCTTCAGCATTGTGCGAT
CACTGGGACACTTTAAGGAACGCACTATATGAACGTAGGATCTCAGAATTGACAGGTAAG
ATAACGAAGTCTGCTAAAGAGAAAGTGCAGAGATCCCTAAAACACGAGGATATAAATTTG
CAGGAGATAATTTCAGCTGCAGGTAAAGAGTTGTCTGAAGCGTTCAAGCAAAAGACTTCC
GAAATCTTGTCACACGCACACGCCGCATTAGATCAACCTTTACCCACTACTTTGAAAAAA
CAAGAAGAGAAGGAGATATTAAAATCACAACTTGATTCTTTACTTGGCCTTTATCATCTT
TTAGATTGGTTCGCTGTTGACGAGAGCAATGAAGTGGATCCAGAGTTTTCCGCAAGATTG
ACCGGTATAAAGTTGGAAATGGAACCTTCGTTATCATTTTACAACAAAGCTAGGAACTAT
GCTACAAAAAAACCTTATTCTGTCGAAAAATTTAAACTGAACTTCCAAATGCCTACTCTA
GCAAGTGGCTGGGATGTTAATAAAGAAAAGAACAATGGCGCTATTTTGTTTGTAAAAAAT
GGCCTATACTATCTTGGAATTATGCCTAAACAAAAAGGTCGCTACAAGGCTTTGTCATTT
GAACCTACTGAAAAGACTAGCGAAGGTTTCGATAAGATGTATTACGATTATTTCCCGGAT
GCCGCTAAAATGATCCCCAAGTGCTCTACTCAATTGAAGGCAGTAACTGCTCATTTCCAA
ACGCATACCACGCCAATACTGCTTTCTAACAACTTTATAGAACCACTAGAAATAACGAAA
GAAATTTACGACCTAAATAACCCAGAGAAAGAACCAAAAAAGTTCCAGACGGCCTACGCC
AAAAAGACAGGGGACCAAAAAGGTTACCGCGAGGCGTTATGTAAATGGATTGATTTTACT
AGGGACTTTTTATCAAAATACACTAAAACGACGTCTATTGATCTTAGCTCCTTACGCCCG
TCCTCCCAATACAAGGATCTAGGTGAGTATTACGCAGAGTTGAACCCGCTATTATACCAT
ATTTCCTTCCAAAGGATTGCTGAAAAGGAAATTATGGACGCTGTTGAAACTGGGAAATTG
TACCTGTTTCAGATTTATAATAAGGACTTCGCAAAGGGTCACCATGGTAAGCCTAACCTT
CACACTTTGTACTGGACCGGACTATTCTCGCCTGAAAATTTGGCTAAAACAAGTATCAAG
TTAAACGGTCAGGCCGAGTTATTTTATAGACCCAAATCTAGAATGAAAAGAATGGCCCAT
AGATTAGGCGAAAAGATGTTAAACAAGAAATTAAAGGACCAAAAAACCCCGATACCAGAC
ACTCTATACCAAGAACTGTACGACTATGTGAATCACAGGCTTAGTCACGATTTATCAGAT
GAAGCGAGGGCTTTATTGCCAAATGTCATCACCAAGGAAGTATCACATGAAATAATTAAG
GATAGAAGGTTCACATCTGATAAATTCTTTTTTCATGTCCCAATTACATTGAATTATCAA
GCAGCGAACTCACCATCTAAATTTAATCAGCGCGTCAACGCCTATTTGAAAGAACATCCC
GAAACACCAATCATCGGCATAGATCGAGGTGAGAGAAACTTAATATATATAACTGTGATT
GATTCTACAGGAAAAATCCTGGAGCAACGATCTTTAAATACCATACAACAGTTTGATTAT
CAAAAAAAGTTGGATAACAGAGAAAAAGAACGTGTTGCCGCTAGGCAGGCTTGGTCTGTG
GTAGGAACAATTAAGGACTTAAAGCAGGGCTATCTGTCCCAAGTTATTCATGAAATAGTC
GATCTGATGATACATTATCAGGCAGTTGTCGTGTTGGAAAATTTGAATTTTGGCTTTAAA
TCAAAAAGAACTGGCATAGCAGAAAAAGCTGTGTACCAGCAGTTTGAAAAGATGTTAATC
GATAAGCTAAACTGCCTTGTTCTTAAAGATTACCCCGCAGAAAAAGTAGGTGGTGTTCTT
AATCCATATCAGTTGACAGACCAATTTACATCCTTTGCGAAAATGGGTACGCAAAGCGGG
TTCTTATTCTACGTACCGGCCCCCTATACTTCTAAGATCGACCCACTAACAGGTTTTGTG
GACCCTTTTGTTTGGAAGACGATAAAGAACCACGAGTCACGCAAACATTTCTTAGAGGGC
TTTGATTTCTTGCACTACGACGTGAAAACTGGTGATTTTATCTTACACTTTAAAATGAAC
AGAAATCTCTCTTTCCAACGTGGACTGCCCGGATTCATGCCGGCTTGGGACATCGTTTTT
GAAAAGAATGAAACGCAGTTTGACGCCAAAGGTACACCATTTATAGCGGGTAAGAGAATT
GTGCCGGTCATAGAAAACCATAGATTTACAGGTAGATATAGGGATCTGTACCCTGCTAAT
GAATTGATTGCATTACTCGAAGAGAAAGGAATTGTGTTTCGAGATGGATCGAATATTTTA
CCTAAGTTGTTGGAAAATGATGATTCACACGCAATTGATACTATGGTTGCCCTCATAAGA
TCGGTATTGCAAATGAGAAACTCAAATGCTGCTACGGGAGAGGATTATATAAACAGCCCC
GTTCGCGATCTTAATGGTGTTTGTTTTGATTCACGTTTTCAGAACCCCGAATGGCCAATG
GATGCCGACGCAAACGGAGCATATCATATTGCTCTTAAAGGCCAACTACTATTAAATCAC
TTAAAGGAATCCAAAGACCTAAAATTGCAAAACGGGATATCTAATCAGGATTGGCTGGCT
TACATACAAGAACTACGTAACTAG
SEQ ATGGCCGTTAAGTCAATCAAAGTGAAACTTAGACTGGATGACATGCCAGAGATTCGTGCG
ID GGGTTATGGAAACTTCATAAGGAAGTTAACGCAGGGGTAAGATATTATACCGAATGGTTA
NO: TCATTACTTCGACAAGAGAATTTGTACAGAAGGTCCCCGAACGGCGACGGTGAGCAAGAA
139 TGCGATAAGACGGCTGAAGAATGTAAGGCAGAACTTTTGGAGCGCCTGAGAGCCCGTCAG
GTTGAAAATGGCCATAGAGGTCCTGCGGGATCTGATGATGAGCTTTTACAGCTAGCTAGA
CAATTGTATGAATTGTTGGTCCCTCAGGCTATTGGGGCTAAAGGAGACGCTCAACAAATC
GCCAGAAAGTTCTTGTCACCTCTGGCTGACAAAGATGCCGTGGGAGGATTAGGTATCGCT
AAAGCAGGTAATAAACCAAGATGGGTTAGAATGAGAGAAGCAGGCGAACCTGGTTGGGAA
GAAGAGAAAGAAAAGGCCGAAACTAGAAAAAGCGCTGACAGAACCGCAGATGTTTTACGG
GCCTTGGCTGATTTTGGACTGAAGCCTTTGATGAGAGTGTATACTGATTCAGAAATGTCT
TCCGTTGAATGGAAGCCCCTAAGGAAGGGACAAGCGGTCAGAACCTGGGATAGGGATATG
TTTCAACAGGCTATTGAAAGGATGATGTCATGGGAATCCTGGAATCAAAGAGTAGGTCAA
GAATACGCTAAACTGGTCGAACAAAAGAATAGATTTGAACAAAAAAATTTTGTAGGTCAA
GAACATTTAGTACATTTGGTTAATCAACTTCAACAAGATATGAAAGAGGCATCTCCTGGT
TTGGAATCAAAAGAACAAACAGCACACTATGTTACCGGCCGAGCTTTGCGAGGTTCTGAC
AAAGTATTTGAAAAGTGGGGGAAATTAGCTCCCGATGCCCCCTTTGATCTATATGATGCT
GAAATTAAAAACGTTCAAAGAAGGAACACTAGACGTTTTGGATCCCATGATCTTTTTGCA
AAGCTAGCTGAGCCAGAATACCAGGCTCTATGGCGTGAAGACGCCTCGTTTTTGACTAGA
TACGCAGTATACAATTCAATACTCAGAAAACTAAACCATGCCAAGATGTTTGCTACATTC
ACCCTGCCCGATGCTACCGCTCATCCTATTTGGACTAGATTTGACAAGTTGGGGGGGAAT
CTACATCAGTACACATTTTTATTTAATGAATTCGGTGAAAGAAGACACGCTATTAGATTC
CACAAGCTCCTAAAGGTTGAAAACGGCGTTGCGAGAGAAGTTGATGATGTAACAGTTCCC
ATTTCTATGTCGGAGCAATTGGATAATCTATTGCCTAGAGACCCTAATGAACCAATTGCT
TTGTACTTTCGTGACTACGGTGCAGAACAACACTTTACAGGTGAATTCGGCGGAGCCAAG
ATTCAATGTAGACGTGATCAACTCGCACACATGCATAGAAGAAGAGGCGCTCGTGATGTT
TATTTAAATGTGTCTGTTAGAGTTCAATCCCAATCGGAGGCTAGAGGTGAAAGAAGGCCA
CCATACGCAGCAGTTTTTAGGTTAGTAGGTGATAATCATAGGGCATTTGTCCACTTCGAC
AAATTAAGTGATTATTTAGCAGAGCACCCTGATGATGGAAAGTTGGGCAGTGAGGGATTA
TTAAGTGGGTTGAGGGTAATGTCTGTAGATCTTGGTCTTCGTACTTCTGCGAGTATCTCT
GTCTTTAGAGTAGCACGTAAGGATGAGTTGAAACCTAATAGCAAAGGAAGAGTCCCGTTT
TTTTTTCCTATTAAGGGTAACGATAACCTGGTGGCCGTGCATGAAAGATCACAACTTTTG
AAATTGCCAGGAGAAACGGAGTCCAAGGACTTGAGGGCAATTAGAGAGGAACGTCAGCGT
ACATTGCGACAGCTGAGAACTCAATTGGCTTATTTGAGGTTGTTGGTTAGGTGTGGTTCC
GAGGATGTTGGCAGAAGAGAAAGGTCTTGGGCCAAATTGATAGAACAACCAGTGGACGCC
GCAAATCACATGACACCAGATTGGAGAGAAGCTTTCGAAAATGAACTCCAGAAATTAAAG
AGCCTACATGGCATATGCTCTGATAAAGAGTGGATGGATGCCGTATACGAATCCGTTCGT
AGAGTCTGGCGCCACATGGGTAAGCAAGTACGGGACTGGAGAAAGGATGTTCGTTCCGGC
GAAAGACCGAAGATAAGGGGGTATGCAAAGGACGTTGTAGGCGGTAATTCTATTGAACAG
ATTGAGTATTTGGAAAGGCAGTACAAATTTCTTAAATCCTGGAGCTTCTTCGGCAAAGTG
TCAGGACAAGTCATCAGGGCTGAAAAAGGTTCCAGATTTGCTATTACGCTAAGGGAACAT
ATTGATCATGCGAAAGAAGATAGACTGAAAAAACTAGCAGATAGAATAATTATGGAAGCA
CTTGGTTACGTCTATGCACTTGATGAAAGAGGCAAGGGGAAATGGGTAGCTAAATACCCG
CCTTGTCAACTTATTTTATTAGAAGAATTAAGCGAGTACCAATTTAACAACGATAGACCT
CCATCCGAAAATAATCAGCTGATGCAATGGTCCCATAGGGGTGTTTTTCAAGAATTGATA
AATCAAGCTCAAGTACACGATTTGCTGGTAGGTACTATGTACGCAGCGTTTTCGAGCCGT
TTTGATGCAAGAACTGGTGCCCCAGGTATCAGATGTCGACGTGTTCCGGCCAGATGTACA
CAGGAACATAACCCTGAGCCATTTCCGTGGTGGCTTAATAAGTTTGTTGTCGAGCACACA
TTAGACGCATGCCCTCTGAGAGCAGATGACCTTATACCCACTGGAGAAGGCGAAATATTT
GTTAGTCCATTCTCTGCAGAAGAAGGTGACTTTCACCAGATACATGCAGACTTAAATGCA
GCACAGAATCTCCAACAAAGGTTGTGGTCGGATTTTGATATTTCGCAAATAAGACTAAGA
TGCGATTGGGGAGAGGTTGATGGAGAATTGGTGCTGATTCCAAGATTAACCGGAAAGCGA
ACTGCCGATTCCTATTCTAACAAGGTGTTTTACACAAATACTGGTGTTACCTATTACGAA
AGAGAAAGGGGTAAGAAGAGACGTAAAGTATTTGCTCAAGAAAAATTGTCAGAAGAGGAG
GCAGAACTGTTAGTAGAAGCAGACGAAGCCAGAGAAAAATCAGTTGTGCTTATGCGTGAC
CCTTCCGGCATTATAAATCGTGGTAATTGGACACGACAAAAAGAATTTTGGTCTATGGTC
AATCAACGTATCGAAGGCTACCTAGTTAAGCAAATCAGGTCTAGGGTTCCACTACAAGAT
AGCGCATGTGAAAATACGGGTGATATATAA
SEQ ATGGCTACTAGATCTTTCATTTTAAAAATTGAACCTAATGAAGAAGTGAAGAAGGGTCTC
ID TGGAAAACTCACGAAGTACTTAATCATGGCATTGCCTATTATATGAATATCCTGAAGCTT
NO: ATTCGTCAAGAAGCTATATACGAGCATCATGAGCAAGATCCTAAGAACCCTAAGAAAGTA
140 AGCAAAGCGGAAATTCAGGCTGAATTGTGGGACTTCGTCTTGAAGATGCAGAAGTGTAAC
AGTTTTACGCACGAAGTTGATAAAGATGTGGTGTTTAATATTTTGAGGGAGCTATATGAG
GAGTTGGTGCCCTCGAGTGTCGAAAAAAAAGGAGAAGCTAATCAGCTGTCAAATAAATTT
TTATATCCTCTGGTGGATCCAAACTCTCAATCAGGTAAAGGCACTGCCAGTAGTGGTCGA
AAACCGAGATGGTATAATTTGAAAATCGCAGGTGATCCATCGTGGGAAGAAGAAAAAAAA
AAATGGGAAGAAGATAAAAAAAAAGATCCCCTTGCCAAAATACTAGGTAAGCTAGCCGAG
TATGGACTTATACCATTATTCATTCCTTTCACGGACTCTAATGAACCAATTGTGAAGGAA
ATCAAATGGATGGAAAAATCACGTAATCAGTCTGTTAGGAGGTTGGACAAAGATATGTTT
ATACAGGCTCTTGAGAGGTTTTTGTCGTGGGAGTCCTGGAATTTGAAAGTGAAAGAAGAA
TATGAAAAAGTGGAAAAGGAGCATAAGACGTTGGAAGAAAGGATTAAGGAAGATATTCAG
GCCTTTAAGAGTCTGGAACAGTACGAAAAAGAAAGACAGGAACAGTTATTGAGAGATACT
CTAAACACTAATGAATATAGGCTTTCCAAGAGGGGCTTGCGAGGATGGAGAGAGATAATT
CAGAAATGGTTGAAAATGGATGAGAACGAGCCATCGGAGAAATATCTAGAGGTGTTTAAA
GATTACCAAAGAAAGCACCCTCGCGAAGCTGGTGATTACTCTGTTTATGAATTCCTTTCG
AAGAAGGAAAATCACTTCATCTGGCGAAATCATCCAGAGTACCCATATTTATATGCTACA
TTTTGCGAAATTGACAAGAAAAAAAAAGATGCTAAACAGCAAGCGACATTCACCCTCGCT
GATCCCATCAACCACCCATTATGGGTCAGGTTCGAAGAGAGATCAGGCTCGAACCTGAAT
AAGTACAGGATCTTGACTGAGCAATTGCATACTGAGAAGTTAAAAAAGAAATTGACGGTC
CAACTTGACAGATTGATTTATCCCACTGAATCTGGTGGATGGGAGGAGAAAGGTAAGGTT
GATATTGTCCTATTGCCTTCTCGTCAATTTTACAACCAAATATTTCTGGACATCGAAGAG
AAGGGTAAACATGCTTTTACCTATAAGGATGAGAGTATTAAATTTCCATTGAAGGGAACG
CTTGGCGGCGCTAGAGTTCAGTTCGATAGAGATCATTTGAGAAGATACCCGCATAAAGTG
GAATCTGGTAATGTAGGTCGGATCTACTTTAACATGACGGTAAATATTGAACCTACCGAG
TCACCAGTCAGTAAGTCTTTAAAGATTCATAGGGATGATTTCCCTAAATTTGTCAACTTC
AAGCCTAAGGAACTAACCGAGTGGATCAAAGACAGTAAAGGCAAAAAGTTAAAGAGCGGT
ATTGAGTCCCTGGAGATAGGTCTTAGAGTCATGTCTATCGATTTGGGTCAAAGACAAGCA
GCCGCAGCATCTATTTTCGAAGTTGTTGACCAAAAACCGGATATCGAGGGGAAATTATTT
TTTCCAATAAAAGGAACTGAGCTATACGCTGTGCATCGCGCATCCTTCAATATAAAACTG
CCAGGAGAAACACTAGTAAAATCTAGAGAGGTCTTGCGTAAAGCACGTGAGGACAATCTC
AAATTAATGAATCAGAAGTTAAATTTCCTTAGGAACGTGTTGCATTTCCAACAGTTCGAG
GACATAACTGAACGCGAGAAAAGAGTCACTAAGTGGATCTCAAGACAAGAAAATAGTGAT
GTGCCATTAGTGTATCAAGACGAACTTATTCAAATAAGAGAGCTAATGTATAAACCATAT
AAAGACTGGGTGGCATTCTTAAAACAATTACACAAGCGGCTTGAAGTAGAAATAGGAAAA
GAAGTAAAGCATTGGAGGAAGAGTCTGTCCGATGGTCGCAAAGGCCTGTACGGGATATCA
CTTAAAAATATTGATGAAATTGACAGAACACGAAAATTTTTGTTAAGATGGTCATTGAGA
CCAACCGAACCAGGTGAGGTTAGAAGGTTGGAACCAGGCCAAAGGTTTGCCATCGATCAA
TTAAACCATCTTAACGCACTGAAAGAAGATAGATTGAAGAAGATGGCGAACACTATTATT
ATGCACGCTCTAGGTTATTGCTATGATGTGAGAAAGAAAAAATGGCAAGCCAAGAACCCT
GCATGCCAAATTATTTTGTTTGAAGATCTTTCTAATTACAATCCATACGAAGAGCGTTCA
CGTTTTGAAAACTCTAAATTGATGAAATGGTCTAGAAGAGAGATTCCGAGACAGGTCGCT
CTACAAGGGGAGATTTACGGTCTTCAAGTCGGTGAGGTTGGTGCTCAATTTTCTTCCAGA
TTTCATGCAAAAACTGGGTCTCCAGGCATTAGGTGTTCGGTCGTTACTAAGGAAAAGTTA
CAGGACAACCGTTTCTTCAAAAATTTGCAACGTGAAGGCCGTTTAACACTTGATAAGATA
GCTGTCCTTAAGGAAGGCGATCTGTACCCAGATAAAGGTGGTGAGAAATTCATATCTTTG
AGTAAAGACAGGAAACTGGTTACAACACACGCCGACATTAACGCAGCTCAGAACTTGCAA
AAGAGATTCTGGACAAGGACCCACGGCTTCTATAAGGTGTACTGTAAAGCTTATCAAGTA
GATGGACAAACGGTTTATATTCCTGAATCAAAGGACCAGAAACAAAAAATTATAGAAGAA
TTTGGTGAAGGATACTTTATCTTGAAGGATGGAGTTTATGAGTGGGGCAATGCAGGTAAG
TTAAAGATAAAGAAAGGTTCATCAAAGCAATCAAGTAGCGAACTGGTCGATTCGGATATT
TTAAAGGATAGCTTTGATCTAGCTAGTGAATTGAAGGGAGAAAAGTTAATGTTATACAGA
GATCCCAGTGGGAATGTATTTCCATCTGATAAGTGGATGGCCGCCGGAGTGTTTTTTGGC
AAATTAGAGAGAATCTTGATTTCTAAACTGACCAATCAATACTCAATTTCGACCATCGAA
GACGACTCTTCAAAACAATCCATGTGA
SEQ ATGCCTACTCGCACCATCAATCTGAAGTTAGTTTTGGGGAAGAACCCAGAAAATGCGACT
ID CTAAGACGGGCACTATTCTCTACACATAGACTTGTCAACCAAGCGACTAAGAGAATTGAA
NO: GAATTTTTACTGTTGTGTAGAGGAGAAGCTTATCGTACCGTAGATAATGAAGGTAAAGAA
141 GCTGAGATCCCACGCCATGCTGTTCAAGAAGAGGCGCTTGCTTTTGCAAAAGCTGCACAA
CGACATAACGGCTGTATCTCCACATATGAGGACCAGGAAATCTTGGATGTGCTTAGACAA
TTGTATGAAAGATTAGTACCTAGCGTCAATGAAAACAACGAGGCTGGGGATGCCCAAGCC
GCTAACGCTTGGGTGAGTCCATTAATGAGTGCAGAGTCCGAAGGTGGACTATCGGTCTAT
GATAAAGTGTTAGACCCGCCGCCAGTATGGATGAAACTCAAAGAAGAGAAAGCGCCTGGT
TGGGAAGCTGCTTCTCAGATTTGGATACAGTCCGACGAAGGTCAATCGCTGCTAAATAAA
CCGGGTAGCCCACCACGTTGGATTAGAAAACTTAGATCTGGTCAACCGTGGCAAGATGAC
TTCGTTTCAGACCAAAAAAAAAAGCAAGATGAACTAACGAAAGGTAACGCACCACTCATA
AAACAATTGAAAGAGATGGGCCTCTTGCCTTTAGTTAATCCCTTTTTTAGACATTTGTTG
GATCCCGAGGGTAAGGGTGTATCCCCATGGGACAGATTGGCCGTAAGGGCCGCGGTGGCG
CACTTCATCTCTTGGGAAAGTTGGAACCACAGAACAAGAGCTGAGTATAACAGTTTGAAA
CTGCGAAGAGATGAATTTGAGGCCGCATCTGATGAATTCAAGGACGATTTTACATTGCTA
CGACAATATGAGGCTAAGCGACATAGTACGCTTAAGTCAATTGCCTTAGCTGATGACTCT
AACCCGTACCGAATTGGTGTAAGGTCCTTGAGAGCCTGGAATAGGGTTAGAGAAGAATGG
ATTGACAAAGGCGCAACCGAGGAACAAAGGGTTACCATCCTTAGTAAGCTTCAAACACAA
TTACGGGGTAAATTCGGTGATCCAGACCTATTTAATTGGCTAGCCCAAGATAGACACGTA
CACCTGTGGTCCCCGAGAGATTCCGTCACGCCCCTCGTAAGGATTAATGCCGTCGACAAA
GTGCTTAGAAGACGTAAGCCTTATGCACTGATGACTTTTGCACATCCGAGATTCCATCCA
AGATGGATTCTATACGAAGCGCCTGGTGGTTCTAACTTGCGACAATACGCTTTAGATTGT
ACTGAAAATGCTCTGCATATTACACTTCCATTACTCGTCGACGACGCCCATGGTACATGG
ATTGAGAAAAAAATCCGCGTACCACTCGCTCCTAGTGGACAAATACAAGATTTAACTTTA
GAAAAACTTGAAAAGAAAAAAAACAGATTATACTATAGATCAGGATTCCAACAATTTGCT
GGATTAGCCGGTGGTGCTGAGGTGTTGTTTCATAGGCCGTATATGGAACATGATGAGAGA
TCAGAAGAATCTCTGTTGGAAAGGCCAGGCGCTGTGTGGTTCAAATTAACCTTAGATGTT
GCTACCCAAGCACCACCTAACTGGTTAGATGGTAAAGGCAGAGTTAGGACACCTCCAGAA
GTTCATCATTTCAAAACCGCTCTGTCAAATAAATCTAAACATACGAGAACCTTGCAACCA
GGATTGAGAGTCCTTTCTGTTGATTTGGGTATGAGAACATTTGCTTCTTGTTCTGTTTTC
GAATTGATCGAAGGTAAACCTGAAACAGGTAGAGCATTCCCTGTTGCTGACGAAAGATCA
ATGGATAGTCCAAATAAGTTATGGGCCAAGCACGAGAGAAGCTTTAAACTAACTCTGCCT
GGAGAAACACCGAGCAGAAAGGAGGAAGAAGAGAGAAGCATTGCTAGGGCAGAGATTTAC
GCGCTGAAAAGAGATATTCAAAGACTGAAATCACTCCTAAGATTAGGTGAGGAAGATAAT
GATAATAGAAGAGATGCTTTGTTAGAGCAATTCTTTAAAGGATGGGGTGAAGAGGACGTA
GTTCCTGGTCAAGCTTTCCCTAGAAGCCTCTTTCAGGGATTAGGCGCTGCACCCTTTAGG
TCAACACCCGAATTGTGGAGACAGCACTGTCAGACGTATTACGACAAAGCGGAAGCTTGC
CTGGCAAAGCATATTTCCGACTGGAGGAAGAGAACTAGACCTCGTCCGACTTCGAGAGAG
ATGTGGTATAAGACAAGATCTTACCATGGTGGCAAAAGTATTTGGATGCTAGAATACTTA
GATGCTGTCCGCAAATTACTACTTTCATGGTCGTTAAGAGGTCGTACTTACGGAGCTATT
AATAGACAAGACACCGCTCGTTTTGGTTCCTTAGCTTCTAGATTGTTGCATCATATCAAC
TCTTTAAAGGAAGACCGCATCAAAACCGGTGCAGATAGTATTGTGCAGGCCGCAAGGGGC
TATATTCCTCTCCCACATGGCAAGGGTTGGGAACAGCGTTATGAACCCTGTCAGTTGATA
TTATTTGAAGATCTAGCTAGGTACAGATTTCGTGTAGACAGACCTCGGAGAGAGAATTCG
CAATTGATGCAGTGGAATCATCGAGCTATAGTAGCAGAAACGACGATGCAAGCTGAACTA
TACGGTCAAATAGTCGAAAATACCGCTGCTGGTTTCTCCTCAAGATTTCATGCTGCAACT
GGTGCTCCTGGTGTCAGATGTCGCTTTTTGTTAGAACGAGATTTCGATAATGACCTACCA
AAGCCGTACTTACTGAGAGAACTAAGTTGGATGTTAGGTAACACAAAGGTTGAATCAGAG
GAAGAAAAATTGCGTCTTCTAAGCGAGAAAATTAGACCAGGTTCATTAGTCCCTTGGGAT
GGGGGTGAACAATTCGCGACATTACACCCGAAAAGACAAACTCTTTGTGTCATTCACGCA
GATATGAACGCTGCTCAAAACCTGCAACGCAGATTTTTCGGAAGGTGTGGGGAAGCCTTT
CGCCTTGTGTGTCAGCCACATGGTGATGATGTTTTGAGGCTAGCGTCTACACCAGGTGCA
AGACTTTTGGGTGCATTACAACAACTGGAAAATGGTCAGGGAGCTTTCGAATTAGTTCGT
GATATGGGTAGCACATCACAAATGAATCGTTTCGTCATGAAGTCGTTGGGCAAAAAAAAG
ATCAAGCCATTACAAGACAATAACGGGGATGATGAACTAGAAGACGTGCTATCTGTTTTA
CCTGAAGAAGATGATACCGGACGAATTACTGTATTTCGGGACTCTTCGGGTATATTCTTC
CCTTGTAACGTTTGGATCCCGGCAAAACAGTTCTGGCCTGCGGTCCGTGCTATGATTTGG
AAGGTTATGGCATCACATTCATTGGGTTAG
SEQ ATGACAAAGTTAAGGCATAGACAGAAGAAGTTAACTCACGATTGGGCGGGGTCTAAAAAG
ID AGAGAAGTTCTAGGGAGCAATGGTAAATTACAGAATCCATTGCTAATGCCCGTCAAAAAA
NO: GGTCAGGTGACAGAATTTCGAAAAGCATTTTCCGCATACGCCCGAGCAACCAAAGGGGAA
142 ATGACGGATGGCAGAAAAAATATGTTTACTCACTCATTTGAACCATTCAAGACCAAGCCT
TCGTTACATCAGTGCGAACTGGCTGACAAAGCCTACCAGAGCTTGCATTCATATTTACCG
GGTTCTTTGGCGCATTTTCTTTTATCTGCCCATGCACTTGGTTTTAGGATTTTTAGCAAA
TCAGGGGAAGCCACTGCATTCCAAGCGTCCTCAAAGATTGAAGCTTACGAAAGCAAGTTA
GCTAGCGAGCTTGCTTGTGTTGATTTGTCTATTCAGAACTTGACTATTTCAACTTTGTTC
AACGCATTAACGACTTCCGTAAGAGGTAAAGGTGAGGAGACATCGGCAGATCCACTGATA
GCTAGATTTTACACCTTACTTACCGGTAAACCACTAAGCAGAGACACTCAGGGCCCAGAA
CGAGATTTAGCCGAGGTGATAAGCAGAAAAATTGCAAGTTCTTTTGGAACTTGGAAGGAG
ATGACTGCCAATCCACTTCAATCTCTTCAATTTTTTGAAGAGGAGTTGCATGCGCTAGAT
GCAAATGTTAGTTTGTCACCTGCCTTCGATGTTCTGATTAAGATGAACGACCTGCAGGGT
GACTTGAAGAACAGAACGATAGTTTTTGATCCAGATGCTCCTGTGTTTGAATATAATGCT
GAGGATCCTGCTGACATCATCATTAAACTGACAGCTAGATATGCGAAAGAAGCAGTGATT
AAAAATCAAAATGTCGGGAATTATGTTAAGAACGCTATTACGACAACTAACGCAAACGGA
CTAGGTTGGTTGCTGAACAAAGGCCTTTCCTTATTGCCTGTCTCCACTGATGACGAACTA
TTGGAGTTTATTGGGGTCGAGAGATCCCATCCTAGCTGTCATGCGTTGATAGAACTTATC
GCTCAGTTAGAAGCACCTGAACTGTTCGAAAAAAATGTTTTTTCTGATACTCGTTCCGAG
GTTCAAGGTATGATAGATTCAGCTGTAAGCAATCATATCGCCAGGCTGTCAAGCTCTCGT
AATTCATTGAGCATGGACTCAGAGGAACTTGAGAGATTGATAAAATCTTTTCAAATTCAT
ACACCACATTGTTCATTATTTATAGGGGCTCAATCCTTATCTCAACAATTGGAAAGCCTA
CCCGAAGCATTGCAGTCAGGAGTGAACAGTGCTGATATTCTGCTCGGCTCAACCCAATAC
ATGTTGACAAATTCTTTGGTCGAGGAGTCAATCGCTACGTATCAGAGAACCTTAAATAGA
ATTAACTACCTGTCCGGCGTTGCAGGACAGATTAACGGTGCTATTAAGAGGAAAGCTATT
GATGGTGAGAAGATACATTTACCCGCTGCTTGGTCAGAGTTAATTTCTTTACCCTTTATT
GGGCAACCAGTGATTGATGTTGAATCAGATTTAGCCCACTTAAAGAACCAATACCAGACA
TTGTCTAACGAATTTGATACGCTGATTTCCGCACTGCAAAAGAATTTCGACTTAAATTTT
AATAAAGCCTTGCTTAATCGAACACAACATTTCGAGGCTATGTGTAGATCAACAAAAAAG
AATGCCCTTTCTAAGCCTGAGATCGTTAGTTATAGAGATTTGCTAGCCAGGTTGACTTCT
TGTCTTTATAGGGGCTCTCTAGTCTTGAGGAGGGCGGGTATAGAAGTACTGAAAAAGCAC
AAGATATTTGAGTCCAACTCTGAATTAAGAGAGCACGTTCATGAAAGAAAACACTTCGTA
TTTGTTTCTCCGCTCGATAGAAAAGCCAAGAAGCTCCTACGTTTGACTGACTCTAGGCCT
GATTTATTGCACGTAATTGATGAAATACTACAACATGATAATTTAGAGAACAAGGATAGA
GAATCTTTGTGGTTAGTTCGATCTGGTTATTTACTGGCCGGCCTACCAGACCAACTCTCC
TCTTCCTTTATAAATCTTCCAATCATTACTCAAAAAGGCGATCGTCGCTTGATAGATCTC
ATTCAATACGACCAAATTAATAGAGATGCTTTTGTGATGTTGGTAACTTCCGCTTTTAAG
TCGAACTTAAGTGGGCTGCAGTACAGAGCAAACAAACAATCTTTTGTGGTTACGCGCACT
TTGTCACCATATTTGGGATCTAAATTGGTTTATGTGCCCAAAGATAAAGATTGGCTGGTC
CCTTCCCAAATGTTCGAGGGGAGATTTGCGGACATTTTGCAATCCGATTATATGGTGTGG
AAGGACGCTGGAAGATTGTGTGTTATTGACACAGCTAAGCATTTGTCTAACATTAAAAAA
TCTGTATTCTCAAGTGAAGAAGTCCTCGCGTTTTTAAGAGAATTGCCACACCGTACGTTT
ATCCAAACTGAGGTCAGGGGTTTAGGGGTGAATGTGGACGGTATTGCATTTAATAACGGG
GATATACCCTCTCTGAAGACGTTTAGCAATTGCGTGCAAGTCAAAGTGAGTCGGACAAAC
ACTAGTCTGGTCCAAACATTAAATAGATGGTTTGAAGGCGGTAAGGTCTCGCCGCCTAGC
ATCCAATTTGAGAGAGCATATTACAAAAAAGATGATCAAATCCACGAGGACGCTGCAAAA
AGGAAGATAAGGTTTCAAATGCCAGCTACAGAGTTGGTACACGCGTCAGACGACGCAGGA
TGGACCCCCTCCTATTTACTTGGTATCGATCCCGGTGAATATGGTATGGGTTTGTCATTG
GTCTCAATAAATAATGGCGAAGTTTTAGATAGCGGATTTATACACATAAATTCATTGATA
AATTTCGCTTCTAAGAAATCAAATCATCAAACCAAAGTTGTTCCGAGGCAGCAATACAAG
TCACCATACGCCAACTATCTAGAACAATCTAAAGATTCTGCAGCAGGAGACATAGCTCAT
ATTTTGGATAGACTTATCTACAAGTTGAACGCCCTACCCGTTTTCGAAGCTCTATCTGGC
AATAGTCAAAGCGCAGCGGATCAGGTTTGGACAAAAGTCCTCAGCTTCTACACCTGGGGA
GATAATGATGCACAAAATTCAATTCGTAAGCAACATTGGTTCGGTGCTTCACACTGGGAC
ATTAAAGGCATGTTGAGGCAACCGCCAACAGAAAAAAAGCCCAAACCATACATTGCCTTT
CCCGGTTCACAAGTTTCTTCTTATGGTAATTCTCAAAGGTGTTCATGTTGTGGACGTAAC
CCAATTGAACAATTGCGCGAAATGGCGAAGGACACATCCATTAAGGAGTTGAAGATTAGA
AATTCAGAAATTCAATTGTTCGACGGTACTATAAAGTTATTTAATCCAGACCCGTCAACG
GTCATAGAAAGAAGAAGACATAATTTAGGGCCATCAAGAATTCCTGTAGCTGATAGAACT
TTCAAAAATATAAGTCCAAGCTCACTAGAATTCAAAGAACTAATAACGATTGTGTCACGG
TCTATACGTCATTCCCCAGAATTTATTGCTAAAAAAAGAGGTATAGGTAGTGAGTACTTT
TGTGCTTATAGTGATTGTAATTCCTCCTTAAATTCAGAAGCAAATGCGGCTGCGAACGTT
GCCCAAAAGTTCCAAAAGCAATTGTTTTTCGAATTATAG
SEQ ATGAAAAGAATCTTGAACTCTTTAAAGGTTGCCGCCCTGCGTTTGTTATTTAGAGGTAAA
ID GGATCTGAACTTGTCAAGACTGTTAAATACCCTTTGGTCTCGCCGGTTCAGGGTGCAGTT
NO: GAGGAGTTAGCTGAGGCGATCCGCCATGATAACCTACATCTGTTTGGTCAAAAAGAAATT
143 GTTGACCTTATGGAAAAGGATGAAGGTACGCAAGTTTACTCAGTGGTTGATTTCTGGTTA
GATACCCTTCGTTTGGGGATGTTTTTCAGTCCATCAGCAAACGCATTAAAAATCACGCTG
GGTAAGTTTAATTCTGATCAGGTTAGCCCTTTTAGGAAAGTGTTAGAGCAGTCTCCATTC
TTCTTGGCTGGTAGGCTGAAGGTTGAACCGGCAGAACGTATATTATCTGTCGAGATCCGT
AAGATTGGGAAGAGGGAAAACAGAGTTGAGAACTATGCTGCTGACGTAGAAACGTGTTTT
ATAGGCCAATTAAGTTCAGATGAGAAACAGTCAATACAAAAATTAGCTAATGATATCTGG
GATAGTAAAGATCATGAAGAGCAAAGAATGTTAAAGGCAGATTTCTTCGCTATCCCTTTG
ATTAAGGATCCAAAGGCTGTGACCGAAGAGGATCCTGAAAATGAAACTGCTGGTAAACAA
AAACCCTTGGAGTTGTGTGTCTGCCTTGTCCCAGAACTTTACACAAGAGGATTCGGGTCA
ATAGCCGATTTTTTGGTTCAACGCTTAACTCTTTTAAGGGATAAAATGTCTACAGATACT
GCAGAAGATTGTTTAGAATATGTCGGGATTGAGGAGGAAAAAGGTAACGGCATGAACTCA
TTGTTGGGAACGTTCTTAAAGAATTTGCAAGGCGATGGATTTGAGCAGATTTTCCAATTT
ATGTTAGGGAGCTATGTCGGTTGGCAAGGGAAGGAAGATGTTTTAAGAGAGAGATTAGAC
TTATTGGCTGAAAAAGTGAAGAGGTTACCGAAACCAAAATTTGCTGGCGAATGGTCTGGT
CATAGGATGTTCTTGCATGGCCAATTGAAGTCTTGGTCTTCAAATTTTTTTAGACTATTT
AACGAGACAAGGGAACTTCTAGAGTCTATTAAGTCAGATATACAGCATGCCACAATGCTA
ATATCATATGTAGAAGAAAAAGGTGGTTATCATCCTCAATTACTTAGTCAATATAGAAAA
CTTATGGAACAACTACCAGCTTTGCGTACCAAGGTATTGGACCCTGAGATTGAAATGACA
CATATGTCCGAAGCAGTTCGCTCTTATATAATGATACATAAATCTGTTGCGGGTTTTTTA
CCGGATTTATTAGAATCATTAGATAGAGACAAGGATCGTGAGTTTCTGCTTAGTATTTTT
CCAAGAATCCCAAAAATTGATAAAAAAACCAAGGAAATTGTAGCTTGGGAACTGCCGGGA
GAACCAGAAGAAGGTTATTTATTTACTGCTAATAACTTGTTCAGAAACTTCTTAGAGAAT
CCGAAACATGTCCCGAGATTTATGGCCGAAAGGATCCCAGAAGATTGGACTCGATTACGC
TCTGCTCCTGTCTGGTTCGATGGAATGGTAAAACAATGGCAAAAAGTCGTTAACCAGTTA
GTAGAATCACCAGGTGCTTTATATCAATTTAACGAATCCTTCTTGAGACAAAGGTTACAG
GCCATGTTAACTGTGTATAAGAGGGACTTACAAACTGAAAAATTTCTTAAACTTTTGGCG
GATGTTTGTAGGCCTCTTGTAGATTTTTTTGGTTTGGGTGGAAATGATATTATTTTTAAG
AGCTGTCAAGACCCAAGAAAACAATGGCAAACCGTTATTCCTCTCTCTGTTCCGGCAGAT
GTCTATACTGCTTGCGAAGGTTTGGCGATTAGACTAAGGGAGACATTAGGATTCGAATGG
AAGAATTTGAAAGGTCACGAGAGAGAAGATTTCTTAAGATTGCACCAGTTATTGGGCAAT
TTACTTTTCTGGATTCGTGATGCTAAATTGGTAGTAAAATTAGAGGATTGGATGAACAAC
CCATGTGTTCAGGAATATGTAGAAGCCCGGAAAGCTATCGATCTTCCACTAGAAATATTC
GGTTTTGAAGTGCCTATCTTCCTGAATGGCTATCTATTTTCGGAGTTGAGACAATTAGAA
CTTTTGCTTAGGAGAAAAAGTGTGATGACTAGCTACAGTGTAAAGACTACTGGATCTCCT
AATAGGCTATTTCAGCTAGTTTATTTACCTCTAAACCCTAGTGACCCCGAAAAGAAGAAC
TCAAATAACTTTCAAGAACGTTTGGATACCCCAACTGGTTTGTCCCGTCGTTTCCTAGAC
CTAACCCTTGATGCATTCGCAGGTAAGTTACTTACCGATCCAGTTACACAAGAATTGAAG
ACAATGGCAGGTTTTTACGATCATCTTTTTGGATTCAAATTGCCATGTAAACTCGCCGCC
ATGTCGAATCATCCAGGTTCTTCTTCAAAGATGGTTGTGTTAGCGAAACCCAAAAAAGGT
GTTGCTTCTAATATAGGGTTTGAACCGATCCCAGATCCCGCTCATCCCGTATTTAGGGTT
AGATCCAGTTGGCCAGAGTTGAAGTACCTCGAGGGGCTATTGTATTTGCCAGAAGACACA
CCTTTGACCATCGAATTAGCAGAGACCTCCGTATCGTGCCAAAGTGTCTCGTCAGTTGCA
TTCGATTTGAAAAACTTGACAACGATCTTAGGTCGTGTGGGAGAATTTAGGGTCACAGCT
GATCAACCCTTTAAACTAACGCCTATAATCCCGGAGAAAGAAGAATCTTTTATTGGTAAA
ACTTATTTGGGTCTCGACGCGGGTGAAAGGAGCGGCGTCGGTTTCGCTATTGTTACAGTG
GACGGAGATGGGTACGAAGTGCAAAGATTGGGGGTCCACGAGGATACACAGCTTATGGCC
TTGCAGCAAGTTGCTAGTAAATCCTTAAAAGAGCCAGTATTTCAGCCTCTAAGAAAAGGC
ACCTTTAGACAACAAGAAAGAATACGGAAATCCTTACGTGGTTGCTACTGGAATTTTTAT
CATGCCTTGATGATAAAATATAGGGCCAAAGTAGTACATGAGGAATCTGTCGGAAGTAGT
GGTCTTGTGGGTCAATGGTTGAGGGCTTTTCAGAAGGATTTGAAGAAAGCCGATGTTCTC
CCCAAGAAGGGCGGTAAAAACGGTGTAGATAAGAAGAAGAGAGAGTCCTCAGCTCAAGAC
ACTCTTTGGGGTGGTGCTTTCTCTAAAAAGGAGGAGCAACAGATTGCGTTTGAGGTGCAA
GCTGCAGGTTCTTCGCAATTTTGTTTGAAGTGCGGATGGTGGTTCCAACTAGGCATGCGT
GAAGTAAACAGGGTACAAGAATCGGGCGTCGTGTTAGATTGGAATAGAAGCATAGTTACC
TTTTTAATAGAATCATCCGGCGAAAAAGTTTATGGTTTCTCCCCACAGCAATTAGAGAAG
GGTTTCAGACCAGACATCGAAACTTTTAAAAAGATGGTAAGAGACTTTATGAGACCTCCT
ATGTTTGATAGAAAAGGCAGACCGGCCGCAGCTTACGAGAGATTTGTTTTAGGAAGGAGA
CATCGAAGGTACAGGTTTGATAAAGTATTTGAGGAAAGATTTGGGAGGTCTGCTCTTTTC
ATTTGTCCTAGAGTAGGTTGTGGAAATTTTGACCACAGCTCCGAACAGTCCGCGGTTGTT
TTGGCCTTGATCGGATATATTGCCGATAAGGAGGGAATGTCAGGTAAGAAGTTGGTTTAT
GTACGGCTGGCCGAACTTATGGCCGAATGGAAACTAAAAAAATTAGAAAGATCCAGAGTT
GAAGAACAATCATCCGCTCAATAA
SEQ ATGGCAGAAAGCAAACAAATGCAGTGTAGGAAATGTGGAGCTAGTATGAAGTACGAAGTC
ID ATCGGTTTGGGTAAAAAGTCATGTAGATACATGTGTCCCGATTGTGGCAACCATACCTCG
NO: GCAAGAAAGATACAAAACAAAAAAAAAAGAGATAAAAAATATGGGTCAGCCAGTAAAGCC
144 CAATCTCAAAGAATTGCTGTAGCAGGTGCTCTTTACCCTGACAAAAAAGTACAAACTATC
AAAACCTATAAATATCCAGCAGACTTGAATGGTGAGGTGCATGATAGCGGTGTTGCCGAG
AAAATCGCACAAGCAATACAAGAGGACGAGATTGGACTTTTGGGACCAAGCTCAGAATAT
GCATGCTGGATTGCATCTCAAAAACAGTCTGAGCCTTACAGTGTAGTCGATTTCTGGTTT
GATGCAGTGTGCGCAGGGGGAGTCTTCGCCTACTCTGGCGCTAGATTATTGAGTACAGTT
TTACAGTTATCCGGTGAGGAATCGGTGCTTAGAGCTGCCTTAGCCTCGTCTCCATTCGTT
GACGATATAAACTTAGCGCAAGCCGAAAAGTTTTTGGCGGTTAGCAGGCGTACAGGTCAA
GATAAGTTAGGTAAGAGAATTGGGGAGTGCTTTGCAGAAGGAAGATTGGAAGCTTTAGGG
ATAAAAGATAGAATGAGGGAATTTGTTCAAGCTATCGATGTTGCACAGACCGCCGGACAA
CGTTTCGCTGCCAAATTGAAGATATTCGGTATAAGTCAGATGCCAGAAGCTAAGCAATGG
AATAACGATTCCGGACTGACTGTCTGTATACTACCTGATTATTATGTTCCCGAAGAGAAT
CGCGCGGACCAACTTGTAGTGTTGTTAAGAAGACTTCGCGAGATTGCATATTGCATGGGT
ATTGAAGATGAAGCGGGTTTCGAACATCTTGGAATAGATCCTGGTGCTCTTTCGAATTTT
TCAAACGGTAACCCTAAGAGAGGATTTCTAGGGAGGCTGTTAAATAACGATATTATTGCG
TTGGCAAACAATATGAGTGCGATGACTCCATATTGGGAAGGGCGTAAGGGTGAACTCATA
GAAAGGCTTGCGTGGTTAAAGCACAGGGCAGAAGGGCTGTATCTTAAAGAACCTCATTTC
GGTAACTCCTGGGCCGATCATAGGTCACGAATTTTCTCAAGGATCGCAGGCTGGTTATCT
GGTTGCGCTGGCAAGTTGAAAATTGCGAAAGACCAAATTTCTGGAGTACGTACAGATCTA
TTTCTGCTAAAAAGACTGCTGGACGCAGTTCCGCAATCGGCGCCATCCCCCGATTTTATT
GCGTCAATTTCGGCACTTGACAGGTTTTTAGAAGCTGCAGAATCGAGCCAGGACCCTGCT
GAACAAGTGAGGGCTCTCTACGCTTTTCACTTGAACGCACCTGCAGTCCGAAGTATAGCC
AATAAAGCAGTGCAAAGGTCCGACAGCCAAGAATGGCTGATAAAAGAACTAGACGCTGTT
GACCATTTAGAATTTAACAAAGCGTTCCCATTTTTCTCTGACACAGGAAAAAAAAAAAAA
AAAGGTGCTAATAGCAACGGTGCTCCATCGGAAGAAGAGTACACTGAAACGGAATCAATA
CAACAACCTGAGGACGCGGAACAGGAAGTAAACGGACAAGAAGGGAACGGAGCGTCTAAA
AATCAAAAGAAATTTCAAAGAATACCTAGATTCTTCGGTGAAGGCTCCAGATCTGAATAC
AGAATTTTAACGGAAGCTCCACAGTATTTCGATATGTTTTGTAATAACATGAGGGCTATA
TTTATGCAGTTAGAAAGTCAACCCCGTAAAGCTCCCAGAGATTTTAAATGTTTCCTACAA
AATCGATTACAAAAATTATACAAACAGACTTTCTTGAATGCACGAAGCAACAAGTGTCGC
GCTCTGCTTGAGTCAGTTTTAATCTCTTGGGGAGAATTTTATACATACGGTGCCAACGAA
AAGAAATTTAGATTAAGACATGAAGCTTCAGAACGCAGCAGTGACCCAGATTACGTAGTT
CAGCAAGCCTTGGAAATCGCGCGTCGTCTATTCCTTTTTGGCTTCGAATGGAGAGATTGC
TCCGCTGGTGAAAGAGTGGATTTGGTTGAAATTCACAAAAAGGCTATCAGTTTTTTGTTG
GCTATTACTCAAGCTGAGGTCTCTGTTGGTTCATACAATTGGCTTGGCAACTCAACAGTA
TCGAGATATTTATCCGTTGCGGGAACTGATACCTTATACGGTACCCAATTGGAAGAATTC
CTGAACGCTACAGTGTTGAGTCAAATGCGTGGTCTGGCCATTAGATTGAGTTCTCAAGAA
CTTAAGGACGGTTTTGATGTGCAGCTCGAGTCTTCCTGCCAGGACAATCTGCAACACCTA
TTGGTGTATAGGGCTTCGAGAGATTTGGCGGCTTGCAAGCGCGCTACTTGTCCAGCCGAA
CTCGATCCTAAGATTTTAGTTTTACCGGTAGGTGCATTCATCGCTTCCGTAATGAAAATG
ATAGAAAGAGGTGACGAACCTTTAGCTGGTGCTTATTTACGGCATAGGCCACACTCTTTC
GGATGGCAAATTAGGGTCCGCGGTGTTGCTGAGGTAGGGATGGATCAGGGTACAGCATTG
GCCTTTCAAAAGCCAACAGAGTCAGAACCTTTTAAAATTAAGCCCTTCTCTGCACAGTAT
GGACCAGTTCTGTGGTTGAACAGTAGTAGTTATTCTCAATCACAATATTTGGACGGTTTT
CTATCTCAACCAAAAAATTGGAGTATGAGGGTGTTGCCTCAGGCGGGTTCAGTTCGCGTC
GAACAACGAGTTGCTTTGATATGGAACTTACAAGCAGGCAAGATGAGACTAGAACGCTCC
GGTGCGAGGGCCTTTTTCATGCCTGTACCGTTTTCATTTAGGCCATCCGGCAGTGGGGAC
GAAGCAGTTTTGGCGCCCAACCGGTACTTGGGTCTGTTCCCTCATTCCGGAGGTATAGAA
TACGCTGTAGTGGATGTCCTGGATTCTGCTGGATTTAAAATTCTTGAAAGAGGCACTATT
GCTGTCAATGGTTTCTCTCAGAAAAGGGGAGAGCGCCAAGAAGAAGCCCATCGTGAAAAA
CAAAGAAGGGGGATAAGTGATATAGGGCGAAAGAAGCCTGTGCAGGCAGAAGTCGATGCG
GCGAACGAATTGCATAGAAAGTACACTGATGTTGCCACAAGATTAGGTTGTAGAATCGTC
GTTCAATGGGCACCACAACCTAAACCAGGGACAGCACCGACAGCGCAAACTGTTTACGCG
AGGGCTGTTAGGACAGAAGCTCCGAGGAGCGGCAACCAAGAAGATCATGCAAGAATGAAA
AGTTCTTGGGGTTACACCTGGGGTACGTATTGGGAGAAACGAAAACCAGAAGATATTTTA
GGGATTTCTACACAGGTGTATTGGACAGGAGGTATAGGCGAATCCTGTCCTGCTGTAGCA
GTCGCTTTATTAGGTCATATTAGAGCAACTTCAACACAAACGGAGTGGGAAAAGGAAGAA
GTTGTCTTTGGAAGACTGAAGAAGTTCTTTCCGAGTTAA
SEQ ATGGAGAAGAGAATTAATAAGATACGGAAAAAATTATCTGCGGATAATGCAACAAAGCCA
ID GTCTCTCGTTCAGGCCCCATGAAAACCCTGCTTGTAAGAGTAATGACGGATGATTTAAAA
NO: AAGAGGTTGGAAAAGCGTAGAAAAAAACCAGAAGTGATGCCGCAAGTGATCTCAAATAAC
145 GCAGCTAATAATCTAAGGATGCTACTTGATGATTATACAAAAATGAAAGAAGCAATCCTG
CAAGTTTACTGGCAGGAATTCAAGGATGACCATGTTGGACTAATGTGCAAATTCGCACAA
CCAGCGTCTAAGAAAATTGACCAAAATAAATTGAAACCCGAAATGGACGAAAAAGGGAAT
TTAACAACTGCCGGGTTTGCCTGCTCGCAATGTGGGCAACCATTATTTGTTTATAAATTA
GAGCAGGTTTCGGAAAAAGGAAAGGCTTACACAAATTACTTCGGCAGATGTAATGTTGCC
GAACACGAAAAACTCATATTGTTAGCTCAGTTGAAGCCTGAGAAAGACTCTGATGAGGCC
GTTACTTACTCGTTGGGGAAGTTTGGTCAAAGAGCTCTCGATTTTTATTCTATTCATGTG
ACAAAGGAGTCCACACATCCCGTCAAGCCCTTGGCACAAATTGCGGGTAATAGATACGCT
TCGGGTCCAGTTGGGAAGGCCCTTTCTGATGCATGTATGGGCACAATTGCTAGCTTTCTT
AGTAAATACCAGGATATCATAATAGAGCATCAAAAAGTTGTAAAGGGTAACCAAAAGAGA
TTAGAATCGCTGCGTGAGTTGGCGGGTAAAGAAAACTTGGAATATCCATCTGTCACTCTG
CCTCCTCAACCTCATACTAAGGAAGGTGTAGATGCGTACAATGAAGTTATCGCTAGAGTC
CGTATGTGGGTGAATTTAAATTTGTGGCAAAAATTGAAGTTATCGCGTGATGATGCAAAA
CCTCTTCTTAGACTAAAGGGCTTTCCTAGCTTCCCTGTAGTGGAAAGACGCGAAAATGAA
GTCGATTGGTGGAATACAATTAACGAAGTCAAAAAACTGATCGATGCAAAGCGAGATATG
GGTCGAGTTTTTTGGTCTGGTGTTACAGCTGAAAAAAGGAATACGATCTTAGAAGGTTAC
AACTACTTGCCAAATGAGAACGATCATAAAAAAAGAGAAGGCAGTTTAGAAAATCCAAAA
AAGCCAGCTAAGAGACAATTTGGTGATTTGCTACTTTACCTAGAAAAAAAGTACGCCGGA
GATTGGGGGAAAGTCTTTGACGAAGCTTGGGAGAGAATAGATAAAAAAATAGCAGGATTG
ACGTCACACATTGAAAGAGAAGAGGCGAGAAATGCAGAAGATGCTCAGTCCAAAGCTGTC
CTCACCGACTGGTTGAGAGCCAAAGCGTCCTTTGTTCTCGAACGCCTAAAAGAAATGGAT
GAGAAGGAATTTTATGCCTGCGAAATCCAGCTACAAAAATGGTACGGAGACTTGAGAGGT
AACCCCTTTGCCGTGGAAGCAGAGAACCGTGTTGTAGATATCTCCGGTTTCTCAATCGGT
AGCGATGGACACTCCATTCAGTATCGCAACTTGTTGGCCTGGAAATATTTGGAAAACGGT
AAGAGGGAATTCTATTTACTTATGAATTATGGCAAGAAAGGTAGAATCAGGTTTACTGAC
GGAACAGACATTAAAAAGAGTGGTAAGTGGCAAGGCCTTTTGTACGGTGGTGGCAAGGCC
AAAGTAATAGACTTAACATTTGACCCCGACGACGAACAACTGATAATACTGCCTTTAGCT
TTTGGTACTCGACAGGGGCGAGAGTTCATTTGGAATGATCTTTTGTCACTCGAGACTGGT
TTGATAAAACTTGCAAATGGAAGAGTCATCGAGAAGACAATTTACAACAAAAAGATAGGT
CGCGATGAGCCTGCACTATTTGTGGCCTTGACCTTTGAGAGAAGGGAAGTTGTCGACCCA
TCCAATATTAAACCAGTCAACCTAATCGGTGTAGATAGAGGTGAAAACATCCCAGCTGTT
ATCGCTCTGACAGACCCTGAAGGTTGCCCTTTGCCAGAATTTAAAGATTCGTCTGGTGGA
CCAACAGATATATTACGTATTGGGGAAGGCTATAAAGAGAAACAACGTGCTATTCAGGCT
GCAAAAGAAGTTGAACAGAGGAGAGCTGGAGGTTACAGTAGAAAATTCGCCAGTAAAAGT
AGAAACTTAGCAGATGACATGGTTAGAAACTCTGCCCGGGATTTGTTCTATCATGCGGTT
ACTCACGATGCAGTCTTAGTCTTTGAAAATCTATCGCGCGGTTTTGGTAGGCAAGGCAAG
AGGACTTTTATGACAGAGAGACAATATACAAAAATGGAAGATTGGTTAACCGCGAAGCTC
GCATATGAAGGTCTTACTTCGAAAACGTACCTCAGCAAAACGCTGGCTCAATATACTTCT
AAAACTTGTTCAAATTGTGGTTTTACTATTACCACGGCAGACTACGACGGGATGTTGGTG
AGATTGAAGAAGACGAGCGATGGTTGGGCAACAACATTGAATAATAAGGAATTAAAAGCA
GAAGGACAGATTACGTATTACAATCGTTATAAACGCCAAACGGTTGAGAAAGAGTTGTCA
GCCGAGTTGGATAGACTAAGTGAAGAGAGCGGTAACAATGATATCTCAAAGTGGACTAAA
GGGAGGCGGGATGAAGCCCTCTTTTTACTAAAGAAGAGATTCTCACATAGACCTGTGCAA
GAACAATTCGTTTGTTTAGATTGTGGCCATGAGGTTCATGCAGACGAACAGGCTGCGTTA
AATATTGCGAGAAGCTGGCTATTTCTAAATTCTAATTCAACAGAGTTCAAGAGCTATAAA
TCCGGAAAACAACCTTTCGTAGGCGCGTGGCAAGCCTTCTATAAAAGGAGATTAAAAGAG
GTTTGGAAACCAAATGCA
SEQ ATGAAAAGAATTAACAAAATTAGAAGGAGGCTGGTCAAAGATTCTAATACCAAGAAAGCT
ID GGTAAGACTGGTCCGATGAAAACCCTATTAGTCAGAGTTATGACCCCAGATTTGAGAGAA
NO: AGATTGGAGAACCTCAGGAAAAAGCCCGAAAACATCCCACAACCCATTAGTAACACATCA
146 AGAGCTAATTTAAACAAGTTATTAACTGACTACACTGAAATGAAAAAAGCAATATTGCAT
GTTTACTGGGAAGAGTTCCAGAAAGATCCTGTTGGGTTGATGTCTAGAGTTGCTCAACCG
GCCCCAAAGAATATAGATCAAAGGAAACTTATTCCTGTGAAGGACGGCAATGAAAGATTA
ACCAGCTCCGGTTTCGCTTGCTCCCAGTGCTGCCAACCCCTGTATGTATACAAACTGGAA
CAAGTAAATGATAAAGGTAAGCCACATACTAACTACTTTGGTAGGTGTAATGTATCCGAG
CATGAAAGATTGATCTTGTTAAGTCCCCATAAACCAGAAGCTAATGATGAGTTAGTAACT
TATAGTTTAGGTAAGTTCGGACAACGAGCTTTAGATTTCTATAGCATCCATGTTACAAGA
GAAAGCAATCACCCCGTCAAACCACTGGAACAAATCGGTGGTAATAGTTGTGCGTCAGGT
CCAGTAGGCAAAGCTTTATCAGACGCTTGCATGGGTGCCGTGGCTAGTTTTTTGACGAAA
TACCAAGATATTATACTGGAACATCAAAAGGTAATTAAAAAGAATGAAAAGAGACTCGCT
AACTTAAAAGATATTGCAAGTGCCAATGGTTTAGCTTTTCCTAAAATTACCTTGCCACCT
CAGCCACATACAAAGGAGGGAATTGAAGCTTACAATAATGTAGTAGCCCAAATAGTTATT
TGGGTGAACCTTAACCTATGGCAAAAGTTAAAAATTGGTAGAGACGAAGCCAAACCCCTG
CAGAGGCTGAAGGGTTTTCCCTCCTTCCCCTTAGTAGAGAGACAAGCTAATGAAGTGGAC
TGGTGGGATATGGTGTGCAATGTTAAAAAATTGATTAATGAGAAGAAAGAGGATGGTAAA
GTGTTTTGGCAGAATCTTGCTGGCTACAAGAGACAGGAAGCTTTACTGCCTTATTTATCT
TCTGAGGAAGATAGGAAAAAAGGTAAAAAATTTGCTAGATATCAATTCGGAGACCTACTT
CTGCATTTAGAAAAAAAACATGGCGAAGATTGGGGTAAAGTTTATGATGAAGCCTGGGAA
AGAATTGATAAGAAGGTAGAAGGTCTCTCCAAACATATTAAATTAGAGGAAGAACGTAGG
TCCGAAGACGCTCAATCAAAGGCAGCATTAACTGATTGGTTGAGAGCAAAAGCCTCTTTC
GTTATTGAAGGATTAAAAGAAGCCGACAAAGATGAATTTTGTAGATGTGAGTTAAAGTTG
CAAAAGTGGTATGGAGACCTCCGTGGTAAACCTTTTGCTATTGAGGCTGAAAATTCTATA
CTCGATATCTCTGGATTTTCAAAACAATATAACTGCGCATTTATATGGCAGAAAGATGGT
GTTAAAAAGCTAAATCTATACTTAATTATCAATTACTTTAAAGGTGGTAAATTGCGTTTT
AAGAAGATAAAGCCTGAAGCCTTTGAGGCAAACCGTTTTTACACTGTTATCAATAAAAAA
TCTGGGGAAATCGTACCAATGGAAGTTAATTTCAATTTCGATGATCCTAATCTTATTATT
TTACCTCTTGCTTTCGGCAAAAGGCAAGGTAGGGAGTTTATTTGGAATGATTTATTGTCG
CTGGAAACGGGGTCTCTCAAACTCGCAAACGGTAGGGTGATAGAAAAAACATTATACAAC
AGGAGAACTCGGCAGGATGAGCCAGCTCTTTTTGTGGCTCTGACATTCGAGAGAAGGGAA
GTTTTAGATTCATCTAACATCAAACCAATGAATTTAATAGGTATTGACCGGGGTGAAAAT
ATACCTGCAGTTATTGCTTTAACTGATCCTGAGGGATGTCCTCTTAGCAGATTCAAGGAC
TCGTTGGGTAACCCTACTCACATCTTAAGGATTGGAGAAAGTTACAAGGAGAAACAAAGG
ACAATACAAGCTGCTAAAGAAGTAGAACAAAGGAGGGCGGGTGGATATAGTCGGAAATAT
GCCAGCAAGGCCAAGAATTTAGCTGACGACATGGTTAGGAATACAGCTAGAGACCTTTTA
TACTATGCCGTCACCCAGGATGCCATGTTGATATTTGAAAATTTAAGTAGAGGCTTCGGT
AGACAAGGTAAGCGCACCTTCATGGCAGAGAGACAATATACTAGAATGGAAGATTGGTTG
ACTGCCAAATTGGCATACGAAGGTCTACCTAGTAAGACGTACTTATCTAAAACACTAGCG
CAGTATACTTCCAAGACATGCAGTAATTGTGGTTTCACAATCACTTCTGCCGATTACGAT
CGCGTCTTGGAAAAACTAAAAAAAACAGCGACAGGTTGGATGACTACTATTAATGGGAAA
GAATTGAAGGTCGAAGGACAAATAACTTACTATAATAGATATAAACGGCAAAACGTTGTA
AAAGACCTGTCAGTCGAACTCGATCGACTTAGTGAAGAATCTGTTAATAATGATATTAGT
TCGTGGACAAAAGGTAGATCCGGTGAAGCTTTGAGCCTCCTGAAAAAACGTTTTAGCCAT
AGGCCTGTCCAAGAAAAGTTTGTATGTTTAAACTGTGGTTTTGAGACCCATGCAGACGAG
CAGGCCGCTCTTAATATTGCTAGATCATGGTTATTTTTAAGATCTCAGGAATACAAGAAG
TACCAGACTAACAAGACAACAGGCAACACAGATAAGCGAGCATTCGTTGAGACTTGGCAA
TCTTTTTATAGAAAGAAATTGAAGGAAGTCTGGAAACCA
SEQ ATGGGAAAAATGTATTATCTAGGCCTGGACATAGGGACCAATTCAGTAGGCTACGCTGTC
ID ACTGACCCCTCCTACCATTTGCTGAAGTTCAAGGGGGAACCCATGTGGGGAGCACACGTG
NO: TTTGCGGCCGGCAACCAGAGCGCAGAGCGGAGAAGCTTCCGCACCTCCAGGAGAAGGCTG
147 GATCGCAGGCAGCAGCGTGTGAAGCTGGTCCAAGAGATATTTGCCCCAGTGATTTCCCCC
ATCGATCCGCGCTTCTTTATTAGGCTCCACGAGTCCGCTCTCTGGCGCGACGACGTGGCC
GAAACTGATAAACATATTTTCTTTAATGACCCAACATACACTGACAAGGAGTACTATTCA
GATTACCCAACAATTCACCATTTGATCGTGGACCTTATGGAAAGTTCGGAGAAGCATGAT
CCTCGACTTGTCTATTTGGCCGTGGCGTGGCTCGTGGCACATAGGGGCCACTTCTTGAAC
GAGGTGGACAAGGATAACATCGGGGATGTGTTATCTTTCGACGCTTTCTATCCTGAATTC
CTTGCTTTTCTGTCTGACAATGGCGTCAGCCCGTGGGTCTGCGAATCCAAGGCCCTCCAG
GCTACGCTATTGTCAAGAAATAGCGTGAACGACAAGTACAAGGCTCTTAAGTCTTTGATT
TTTGGAAGCCAGAAGCCCGAGGACAACTTTGATGCAAATATCTCGGAGGACGGGCTGATT
CAGCTCCTCGCTGGGAAAAAGGTCAAGGTCAATAAGCTGTTTCCACAGGAGTCAAATGAC
GCGAGCTTCACCCTTAACGACAAAGAGGATGCCATTGAAGAGATCCTGGGGACACTCACC
CCAGACGAGTGCGAGTGGATAGCCCATATTAGGCGCCTCTTTGATTGGGCCATAATGAAA
CATGCGCTTAAGGACGGGCGCACGATATCCGAAAGCAAGGTCAAATTGTACGAGCAGCAC
CACCATGATCTGACCCAGCTAAAATATTTTGTAAAAACATATCTGGCCAAGGAGTACGAT
GATATCTTCCGCAACGTGGATAGTGAGACCACCAAAAACTACGTCGCGTACTCATACCAC
GTGAAAGAAGTTAAGGGCACGCTGCCTAAGAACAAGGCAACACAAGAGGAGTTCTGCAAG
TACGTTCTCGGGAAAGTTAAAAATATAGAGTGCAGCGAGGCCGACAAAGTGGATTTTGAC
GAGATGATTCAACGCCTGACCGACAATTCGTTTATGCCTAAACAGGTGAGTGGAGAGAAT
CGCGTGATTCCATATCAGCTCTATTACTATGAACTCAAGACTATTCTGAATAAGGCCGCT
AGCTATTTACCCTTCCTTACGCAGTGCGGGAAGGATGCCATTTCTAACCAGGATAAACTC
TTGAGTATAATGACATTTCGAATTCCCTATTTCGTGGGTCCGCTTCGTAAGGATAACAGT
GAGCACGCTTGGCTGGAGCGGAAGGCTGGCAAAATTTATCCATGGAATTTCAACGACAAG
GTGGATCTGGACAAATCCGAAGAAGCCTTTATCCGCAGGATGACCAATACTTGCACATAC
TATCCTGGGGAGGATGTCCTTCCACTGGACTCTCTGATCTACGAAAAGTTCATGATTTTG
AATGAAATTAACAACATAAGGATCGATGGGTATCCTATTTCCGTCGACGTGAAGCAGCAG
GTGTTCGGGCTCTTTGAGAAGAAGCGACGGGTGACCGTGAAGGATATTCAGAATCTTCTC
TTATCGCTGGGAGCCCTGGATAAACACGGAAAACTGACCGGGATAGATACTACGATTCAT
TCTAATTACAACACGTATCACCATTTTAAGTCACTGATGGAGAGGGGCGTCCTAACAAGA
GATGACGTGGAGAGAATAGTGGAACGAATGACATATTCTGATGACACCAAGAGAGTGCGG
CTTTGGCTGAATAACAACTACGGCACTCTGACGGCGGATGATGTAAAGCATATTTCCCGA
CTCCGTAAGCATGACTTCGGGCGGCTGTCTAAGATGTTTCTAACAGGCCTCAAGGGTGTG
CATAAGGAAACTGGGGAGCGCGCTAGCATCCTGGATTTTATGTGGAACACCAATGATAAC
CTGATGCAGCTCCTGTCAGAATGCTACACATTTTCGGACGAAATCACCAAGCTGCAGGAG
GCTTACTATGCCAAGGCCCAACTAAGCTTGAATGATTTCCTGGATTCTATGTACATCAGC
AACGCCGTAAAACGACCAATTTATAGGACACTGGCAGTGGTTAACGACATTAGGAAAGCA
TGCGGAACAGCTCCCAAGCGAATCTTTATCGAGATGGCCCGCGACGGCGAGAGTAAGAAG
AAAAGGTCAGTGACTAGGCGGGAGCAGATCAAGAACCTTTACCGCTCTATCCGAAAAGAC
TTCCAGCAAGAGGTTGATTTCCTTGAGAAGATCTTAGAGAACAAGTCAGATGGACAGCTC
CAATCCGATGCTCTGTATCTGTACTTCGCTCAGCTGGGACGAGATATGTACACTGGCGAC
CCCATTAAACTAGAACATATCAAGGACCAATCGTTTTATAATATCGACCACATCTACCCT
CAGTCCATGGTGAAAGACGATAGTCTGGACAATAAGGTGCTCGTCCAAAGTGAGATTAAC
GGAGAAAAGTCGAGCAGATATCCTTTGGACGCTGCGATCCGCAACAAGATGAAGCCCCTG
TGGGATGCTTACTACAATCATGGACTGATCAGCCTGAAGAAGTATCAGAGACTGACCCGG
AGTACCCCTTTCACAGACGATGAGAAGTGGGATTTTATCAATAGACAACTGGTGGAAACC
AGGCAGTCCACGAAAGCTCTGGCCATTCTTCTGAAGAGAAAGTTTCCAGACACAGAGATC
GTCTATTCAAAGGCCGGCCTCAGTTCCGACTTTAGACATGAGTTCGGACTCGTTAAATCA
CGAAATATAAACGATCTCCACCATGCAAAGGACGCATTCCTCGCGATTGTGACTGGAAAT
GTCTATCACGAAAGATTTAATAGGCGGTGGTTCATGGTTAACCAGCCATACTCAGTGAAG
ACCAAGACCCTTTTCACTCACTCTATTAAAAATGGCAACTTCGTGGCTTGGAATGGTGAG
GAGGATCTTGGAAGAATTGTGAAGATGTTAAAACAGAATAAGAATACCATCCACTTTACT
AGATTCAGCTTTGACCGAAAAGAGGGGCTATTCGATATTCAACCGTTAAAGGCTTCAACA
GGTCTCGTTCCACGAAAGGCCGGACTGGACGTAGTGAAATACGGCGGCTATGATAAGAGC
ACCGCAGCTTACTACCTCCTTGTGCGATTTACGCTCGAGGATAAGAAGACCCAACACAAG
CTGATGATGATTCCCGTGGAGGGACTGTACAAAGCTCGAATTGACCATGATAAAGAGTTT
CTCACAGATTACGCACAAACCACCATCTCTGAGATTCTCCAGAAAGACAAACAAAAAGTT
ATAAACATAATGTTTCCAATGGGTACAAGGCATATTAAACTGAACAGCATGATCTCCATT
GATGGCTTTTATTTGTCCATTGGAGGAAAGTCTAGTAAAGGCAAGTCTGTCCTCTGCCAT
GCCATGGTACCCCTAATCGTCCCACACAAGATTGAATGCTACATCAAGGCTATGGAGAGT
TTTGCTCGGAAATTTAAAGAGAATAATAAGCTGCGTATTGTGGAAAAATTCGACAAGATA
ACCGTTGAAGACAATCTGAATCTGTACGAGCTCTTTCTGCAGAAGCTGCAGCATAACCCC
TATAATAAGTTCTTCTCCACACAGTTCGATGTACTGACCAACGGGCGATCAACTTTCACA
AAGCTAAGTCCTGAGGAACAGGTGCAAACACTCCTAAACATTCTTTCCATTTTTAAGACC
TGCAGATCTTCAGGATGCGACTTGAAGAGCATTAACGGGAGCGCACAGGCAGCTAGGATC
ATGATCTCAGCTGACCTGACAGGGCTGAGTAAAAAATACTCCGACATTCGGCTTGTAGAG
CAAAGCGCCAGTGGGTTGTTCGTTAGTAAGTCGCAGAACCTGCTGGAATACCTGTAA
SEQ ATGTCTTCTTTGACGAAGTTTACAAACAAATACTCTAAGCAGCTTACAATTAAGAACGAA
ID CTGATTCCCGTAGGAAAGACTCTGGAAAACATCAAAGAGAATGGGCTGATAGACGGCGAC
NO: GAACAACTGAATGAGAACTATCAGAAGGCCAAAATTATCGTGGATGACTTCCTGAGGGAT
148 TTTATTAACAAGGCCCTGAATAATACCCAGATCGGCAATTGGCGGGAACTGGCCGACGCT
CTGAACAAAGAAGATGAGGACAATATCGAAAAATTACAAGACAAAATCAGGGGCATTATT
GTCAGTAAGTTCGAGACATTCGATCTGTTCTCTTCGTACTCCATTAAGAAGGACGAGAAA
ATCATCGATGATGACAATGACGTTGAGGAAGAAGAACTGGACTTGGGTAAAAAGACCTCA
TCCTTCAAGTATATTTTTAAAAAAAATCTGTTTAAATTAGTGCTCCCCAGTTATTTAAAG
ACAACTAACCAGGACAAGCTTAAGATTATCTCCTCTTTTGACAACTTTAGCACCTATTTT
AGAGGCTTCTTTGAAAATCGCAAGAATATTTTCACTAAGAAGCCCATAAGCACCTCTATT
GCCTACAGAATCGTACATGATAACTTCCCAAAATTTTTGGATAACATTAGATGTTTTAAT
GTATGGCAGACCGAATGTCCTCAGTTAATTGTGAAGGCGGATAACTACCTCAAATCCAAG
AATGTGATCGCCAAAGATAAGTCTCTTGCTAACTACTTTACGGTCGGAGCCTACGATTAC
TTCTTATCTCAAAACGGTATTGACTTTTACAATAACATTATCGGGGGATTGCCTGCCTTC
GCCGGCCATGAGAAAATTCAGGGCTTAAACGAGTTCATAAATCAGGAATGTCAAAAGGAC
TCAGAGCTGAAATCAAAGCTTAAGAATCGACACGCATTTAAAATGGCGGTCTTGTTCAAA
CAGATCCTCAGCGATAGAGAGAAAAGCTTCGTTATTGATGAATTCGAGAGCGACGCACAG
GTGATTGATGCCGTGAAGAACTTCTATGCGGAACAGTGTAAAGACAATAATGTTATTTTC
AACCTATTAAACTTGATTAAGAATATCGCGTTTTTAAGTGACGATGAACTCGACGGTATC
TTTATAGAAGGCAAGTACCTGTCCTCTGTCAGCCAAAAACTCTACTCAGATTGGTCCAAG
CTAAGAAATGACATCGAGGACAGTGCTAACAGCAAACAGGGCAATAAAGAGCTGGCAAAG
AAAATCAAGACTAATAAAGGGGATGTGGAGAAGGCGATATCTAAATATGAGTTCTCCCTC
TCCGAACTGAACTCCATCGTCCACGATAATACCAAGTTTAGTGATCTGTTGTCGTGTACA
CTGCACAAAGTGGCCAGTGAAAAACTCGTCAAGGTGAACGAAGGCGATTGGCCCAAACAC
CTGAAAAATAATGAGGAGAAACAGAAGATCAAAGAACCTTTGGATGCGTTGCTCGAAATA
TATAACACACTGTTGATCTTCAACTGTAAAAGCTTCAACAAGAACGGGAACTTTTATGTA
GACTACGATCGATGTATAAATGAACTGAGCAGCGTCGTTTACCTGTACAACAAGACTCGC
AATTATTGTACGAAAAAACCATATAACACCGATAAGTTCAAGCTTAATTTCAACAGTCCC
CAGCTGGGAGAAGGGTTCAGCAAATCAAAAGAAAACGATTGCCTGACATTACTCTTTAAA
AAGGATGATAATTATTATGTTGGGATTATTAGGAAAGGCGCTAAGATCAACTTTGACGAC
ACACAGGCCATAGCTGACAACACTGATAACTGCATCTTTAAAATGAATTACTTTCTGTTG
AAGGACGCCAAAAAATTCATTCCAAAATGCTCTATTCAGCTCAAGGAGGTTAAGGCCCAT
TTCAAGAAGTCTGAAGATGACTACATCCTCTCTGACAAGGAAAAATTCGCTAGTCCTCTG
GTTATCAAAAAAAGTACCTTCTTGCTGGCTACAGCTCACGTGAAAGGCAAGAAAGGGAAC
ATTAAGAAGTTCCAAAAGGAATACAGCAAAGAGAATCCAACCGAGTACAGAAATTCTCTG
AACGAATGGATCGCATTCTGTAAAGAATTTCTAAAGACGTACAAGGCCGCTACCATTTTC
GATATTACCACCTTGAAAAAAGCCGAGGAGTACGCCGACATCGTCGAATTCTATAAAGAC
GTGGATAACCTGTGTTACAAATTGGAATTCTGCCCAATTAAGACCTCTTTCATTGAAAAC
CTCATCGACAATGGGGACCTCTACTTATTTAGAATTAACAATAAGGATTTTTCTTCGAAA
TCTACCGGAACTAAAAATCTGCACACACTGTATCTGCAAGCAATCTTCGATGAACGTAAT
CTCAACAACCCTACAATAATGCTGAACGGCGGTGCTGAACTGTTCTACCGTAAAGAGAGT
ATTGAACAGAAGAATCGAATCACACACAAAGCGGGCAGTATTCTCGTCAATAAGGTGTGC
AAAGACGGGACCAGCCTGGACGATAAGATCAGGAATGAAATATATCAGTATGAGAACAAG
TTTATCGACACCTTGTCGGATGAGGCAAAGAAGGTGCTACCTAACGTTATCAAGAAGGAA
GCTACCCATGACATAACCAAGGATAAGCGGTTCACTTCTGACAAGTTCTTCTTCCACTGT
CCTCTGACCATTAACTACAAGGAAGGAGACACTAAACAATTCAATAATGAAGTACTTAGC
TTTTTGCGGGGTAATCCCGATATTAACATAATTGGTATCGACCGGGGAGAACGGAACCTG
ATATACGTGACAGTAATTAATCAGAAAGGAGAAATCCTGGATTCCGTATCCTTCAATACC
GTGACTAATAAATCTAGTAAAATCGAGCAGACGGTCGACTACGAGGAAAAGTTAGCAGTC
AGAGAGAAGGAGAGAATCGAGGCCAAACGTTCCTGGGATAGTATCAGCAAGATTGCTACT
CTGAAAGAAGGATATCTGTCCGCTATCGTCCATGAGATCTGTTTGTTGATGATCAAGCAC
AATGCTATAGTGGTTCTGGAGAACCTGAACGCAGGCTTCAAGCGAATTAGAGGGGGCCTG
TCGGAAAAAAGCGTTTACCAGAAGTTTGAAAAGATGCTAATCAATAAGTTAAATTACTTT
GTAAGTAAAAAAGAAAGCGATTGGAATAAGCCATCAGGACTTTTAAACGGGCTGCAACTG
AGCGACCAGTTTGAGTCATTCGAAAAACTGGGTATTCAGAGTGGTTTCATATTCTACGTA
CCTGCCGCTTACACTTCAAAGATCGATCCTACAACTGGTTTTGCGAATGTCCTGAATCTG
TCTAAGGTGAGGAATGTGGACGCAATCAAGTCTTTCTTCAGCAACTTCAACGAGATATCT
TACAGCAAGAAAGAGGCTCTGTTTAAATTCAGTTTTGATCTGGATAGCCTGAGCAAGAAA
GGATTCTCTTCTTTCGTAAAGTTTTCTAAGTCCAAATGGAACGTCTACACGTTCGGAGAG
AGAATCATTAAACCAAAGAACAAGCAGGGGTATCGGGAAGACAAAAGGATCAATCTGACT
TTCGAAATGAAGAAACTATTGAATGAGTACAAAGTCTCATTCGATTTGGAGAACAATCTG
ATCCCCAATCTGACCAGCGCTAACCTCAAAGACACATTCTGGAAGGAGCTGTTTTTCATC
TTTAAGACCACCCTGCAGCTACGGAATAGTGTCACAAATGGGAAAGAGGATGTACTGATC
TCACCTGTGAAAAACGCCAAGGGGGAGTTCTTTGTGTCCGGCACCCATAACAAAACCCTG
CCTCAGGACTGTGACGCGAACGGGGCCTACCACATCGCGCTAAAGGGGTTAATGATTCTC
GAACGTAATAATCTGGTGCGCGAAGAAAAAGACACAAAGAAAATTATGGCCATCAGCAAC
GTTGACTGGTTTGAGTACGTGCAGAAGCGTCGAGGAGTTTTGTAA
SEQ ATGAACAACTATGACGAGTTCACTAAACTTTACCCCATTCAGAAAACCATCAGATTTGAA
ID CTGAAGCCTCAGGGTCGTACCATGGAACACTTGGAAACTTTCAACTTTTTCGAGGAGGAC
NO: AGGGATAGAGCTGAGAAATACAAGATCTTGAAAGAGGCCATCGACGAGTATCACAAAAAA
149 TTCATCGATGAGCATCTCACCAACATGTCGCTGGATTGGAACAGTCTCAAGCAGATTTCC
GAGAAGTACTATAAATCTCGGGAGGAGAAAGATAAAAAGGTGTTTTTGAGCGAGCAAAAG
CGAATGCGACAGGAGATAGTCTCTGAATTTAAGAAAGATGATCGGTTTAAAGACCTATTT
TCCAAAAAGCTTTTTTCAGAGCTGCTGAAGGAAGAGATCTATAAAAAAGGCAATCACCAA
GAAATTGATGCCCTGAAATCATTCGACAAATTCAGTGGGTATTTCATAGGACTGCATGAG
AACCGGAAGAATATGTATAGTGATGGAGACGAGATCACAGCCATAAGCAATCGAATCGTT
AACGAGAATTTCCCGAAGTTCCTGGATAACCTGCAGAAGTATCAAGAGGCTAGGAAAAAG
TACCCTGAGTGGATCATCAAGGCTGAATCAGCTCTGGTGGCTCACAATATCAAGATGGAT
GAAGTCTTTAGTCTTGAGTACTTTAATAAAGTCCTTAACCAGGAGGGCATCCAGCGCTAT
AACCTGGCTCTCGGTGGCTACGTCACAAAAAGCGGAGAAAAGATGATGGGTCTCAACGAT
GCACTGAATTTGGCTCATCAGTCGGAGAAGTCATCTAAGGGACGCATACACATGACACCA
CTGTTTAAACAAATCCTGAGCGAAAAGGAATCATTTTCCTACATTCCCGACGTATTCACC
GAGGACTCACAACTGCTGCCTAGTATAGGGGGGTTTTTCGCTCAGATAGAGAACGACAAA
GATGGCAACATTTTTGACAGAGCCTTGGAGTTGATTTCATCTTACGCCGAGTACGATACG
GAGCGCATTTATATTCGCCAGGCGGATATCAACAGGGTTTCCAATGTGATCTTTGGCGAG
TGGGGAACGCTGGGCGGGCTGATGCGGGAATACAAAGCCGACTCGATCAATGACATCAAC
CTGGAGAGAACATGCAAGAAGGTCGATAAATGGTTGGATAGCAAAGAGTTCGCCCTGAGT
GACGTCTTGGAAGCTATCAAAAGAACCGGAAATAATGACGCGTTCAACGAGTATATCTCT
AAAATGAGGACCGCGAGAGAAAAAATTGATGCAGCAAGGAAGGAGATGAAGTTTATATCT
GAGAAGATCTCAGGCGATGAAGAGTCCATCCATATTATTAAAACTCTTCTGGACTCAGTG
CAGCAATTCCTGCACTTTTTTAACCTCTTCAAGGCCAGGCAGGATATACCGTTAGACGGG
GCTTTTTATGCCGAGTTTGATGAAGTTCATTCGAAACTTTTTGCTATAGTGCCTCTCTAT
AATAAAGTTCGCAATTACCTGACAAAGAATAACTTAAACACAAAGAAAATCAAGCTCAAC
TTCAAAAACCCAACACTGGCAAACGGATGGGATCAGAACAAGGTATATGATTACGCCTCA
TTGATTTTCCTCCGGGACGGGAATTACTATCTGGGGATCATCAACCCTAAGCGCAAAAAG
AACATTAAGTTCGAACAGGGATCTGGCAATGGTCCCTTCTATAGGAAAATGGTATACAAA
CAGATTCCTGGCCCCAACAAGAATCTCCCACGCGTCTTTCTGACGTCCACTAAGGGAAAG
AAGGAGTACAAGCCGTCTAAAGAAATTATCGAGGGCTATGAGGCAGACAAGCATATTAGG
GGTGACAAGTTTGACCTAGACTTTTGTCATAAGCTTATCGACTTTTTCAAGGAGTCCATA
GAGAAGCACAAAGATTGGTCAAAGTTTAATTTCTATTTTTCTCCAACAGAGTCCTACGGG
GATATCTCTGAGTTCTATCTGGATGTTGAAAAGCAGGGGTACAGAATGCACTTCGAAAAT
ATCTCAGCAGAAACTATCGATGAGTACGTAGAGAAAGGAGATCTGTTTCTTTTCCAAATC
TACAATAAGGATTTTGTGAAGGCCGCCACTGGGAAGAAGGACATGCACACTATTTACTGG
AACGCTGCATTTTCCCCTGAAAATCTGCAGGACGTAGTAGTGAAATTAAATGGTGAGGCA
GAACTGTTTTACCGCGATAAATCAGACATCAAGGAAATAGTGCACCGGGAAGGCGAGATT
CTTGTTAACCGAACATATAATGGCAGGACACCTGTCCCTGATAAAATTCATAAGAAACTG
ACCGATTACCACAACGGTCGAACCAAGGATCTGGGCGAGGCCAAGGAATACCTCGATAAG
GTGAGGTACTTCAAAGCCCATTATGACATCACCAAGGACCGAAGATACCTTAACGACAAA
ATCTACTTCCATGTCCCACTCACCTTGAACTTCAAAGCTAACGGTAAGAAGAACCTCAAT
AAAATGGTGATTGAAAAATTTCTGTCCGATGAGAAGGCCCATATCATCGGCATTGATCGC
GGCGAGAGAAATCTCCTTTACTATTCTATCATTGATCGGTCGGGAAAGATTATCGACCAA
CAATCACTGAATGTCATCGACGGATTCGACTATAGAGAGAAGCTGAACCAACGGGAAATC
GAGATGAAGGACGCGCGCCAGTCCTGGAACGCTATCGGCAAAATTAAAGATTTGAAAGAA
GGTTACCTCTCCAAAGCAGTGCACGAAATTACCAAAATGGCAATCCAGTACAATGCTATT
GTGGTAATGGAGGAGTTAAATTACGGATTTAAGCGCGGGAGGTTCAAGGTTGAAAAGCAA
ATTTACCAAAAATTTGAGAACATGTTGATTGATAAGATGAACTACCTGGTGTTCAAGGAC
GCACCTGACGAGTCGCCAGGCGGCGTGTTAAATGCATATCAGCTGACAAATCCACTGGAG
AGCTTTGCCAAGCTAGGAAAGCAGACTGGCATTCTCTTTTACGTCCCTGCAGCGTATACA
TCCAAAATTGACCCCACCACTGGCTTCGTCAATCTGTTTAACACCTCCTCCAAAACCAAC
GCACAAGAACGGAAAGAATTTTTGCAAAAGTTTGAGTCCATTAGCTACTCTGCCAAAGAC
GGCGGGATCTTTGCTTTCGCATTCGACTACAGGAAATTCGGGACGAGTAAGACAGACCAC
AAGAACGTCTGGACCGCGTACACTAATGGGGAACGCATGCGCTACATCAAAGAGAAAAAG
AGGAATGAACTTTTTGACCCTTCAAAGGAAATCAAGGAAGCTCTCACCTCAAGCGGTATC
AAATACGATGGCGGGCAGAATATTTTGCCAGATATCCTCAGATCGAACAATAATGGACTT
ATCTATACTATGTACTCCTCCTTCATTGCAGCAATTCAAATGAGAGTGTACGATGGAAAG
GAGGATTACATTATATCGCCAATTAAGAACTCCAAAGGCGAATTCTTCCGCACGGATCCT
AAGCGAAGAGAACTCCCAATCGACGCTGATGCGAACGGCGCCTATAATATAGCCCTGCGG
GGTGAATTAACAATGCGCGCTATTGCCGAGAAGTTCGACCCCGATTCAGAAAAAATGGCT
AAGCTTGAGCTGAAACACAAAGATTGGTTCGAATTCATGCAGACAAGAGGCGACTAA
SEQ ATGACTAAGACCTTCGATTCCGAGTTCTTCAACCTTTATTCCCTGCAGAAAACTGTAAGG
ID TTTGAGCTGAAGCCGGTGGGCGAGACAGCCAGCTTCGTAGAGGATTTCAAGAATGAGGGT
NO: CTCAAACGGGTAGTTAGTGAGGATGAGAGGAGAGCAGTGGACTATCAGAAGGTGAAAGAG
150 ATCATCGATGACTATCACCGGGATTTCATAGAGGAGTCGTTGAATTACTTCCCTGAGCAA
GTATCCAAAGACGCGCTGGAACAGGCCTTTCATCTTTACCAGAAACTGAAGGCAGCGAAG
GTTGAGGAGCGGGAAAAGGCCTTGAAAGAGTGGGAAGCCCTGCAGAAAAAGCTCAGAGAA
AAGGTTGTCAAATGCTTCAGCGACAGCAACAAAGCCAGGTTCAGTAGGATCGATAAGAAA
GAACTGATCAAAGAAGACTTGATCAATTGGCTGGTTGCACAGAACCGGGAAGATGATATT
CCCACCGTAGAGACCTTCAACAACTTCACAACTTACTTCACCGGCTTCCATGAGAATCGT
AAAAACATCTACAGTAAAGATGATCATGCAACCGCCATCTCCTTCCGGTTGATCCACGAG
AATCTCCCCAAGTTCTTTGACAACGTGATAAGTTTCAATAAGTTGAAAGAGGGATTTCCC
GAACTCAAGTTCGATAAAGTGAAGGAGGATCTGGAAGTGGATTATGACCTTAAGCACGCT
TTCGAGATAGAGTACTTCGTGAACTTTGTGACTCAGGCCGGCATCGATCAGTATAACTAC
CTCCTCGGGGGTAAGACGCTCGAGGACGGTACTAAGAAGCAAGGAATGAATGAGCAAATT
AATCTATTTAAACAGCAGCAGACCAGGGATAAGGCTAGACAGATCCCCAAGCTTATTCCT
CTTTTTAAACAGATCCTAAGTGAAAGGACAGAAAGTCAAAGCTTCATACCTAAGCAATTT
GAAAGTGATCAGGAGCTGTTTGACTCCCTGCAAAAGCTGCACAACAATTGCCAGGACAAG
TTTACCGTGCTGCAGCAGGCTATCCTCGGACTGGCTGAGGCGGATCTTAAGAAGGTATTC
ATTAAGACTAGCGACCTCAATGCCCTTAGTAACACCATCTTTGGAAATTACTCCGTTTTC
AGCGATGCCCTCAATCTATACAAAGAGAGCTTGAAGACTAAAAAAGCTCAGGAAGCTTTT
GAAAAATTACCGGCACATTCTATACACGACCTTATACAATACTTAGAGCAGTTCAACAGC
AGCCTCGACGCTGAGAAACAGCAATCCACAGACACCGTCCTGAATTACTTCATCAAAACC
GATGAACTGTACTCCCGATTTATCAAGAGCACTTCAGAAGCCTTCACGCAAGTTCAGCCT
CTGTTCGAGCTGGAGGCACTGTCCAGCAAGAGACGACCGCCAGAGTCTGAAGACGAGGGA
GCCAAGGGTCAAGAGGGGTTTGAACAGATAAAGCGAATTAAGGCTTACTTGGATACTCTC
ATGGAGGCGGTGCATTTCGCTAAGCCTTTGTACCTGGTTAAAGGCCGAAAAATGATTGAG
GGGCTAGATAAGGATCAGTCTTTTTACGAGGCTTTTGAAATGGCCTACCAGGAATTGGAA
TCCTTGATCATTCCAATCTATAATAAAGCCCGGAGTTATCTGAGCAGGAAGCCCTTCAAA
GCCGACAAGTTCAAAATAAATTTTGACAATAATACGCTACTGTCTGGTTGGGACGCTAAC
AAGGAAACAGCCAATGCTTCCATCCTGTTTAAGAAAGACGGCCTGTACTACCTGGGAATT
ATGCCAAAAGGCAAAACTTTTTTGTTCGATTACTTTGTGTCATCAGAGGATAGCGAGAAG
TTAAAGCAAAGACGGCAGAAGACCGCCGAAGAAGCCCTCGCACAAGACGGAGAATCATAT
TTCGAGAAAATTCGATATAAGCTCCTGCCTGGCGCATCAAAGATGTTGCCAAAAGTCTTC
TTTTCCAACAAAAACATCGGCTTTTATAACCCCAGCGATGATATCCTTCGCATCCGGAAC
ACCGCCTCACATACCAAAAATGGAACTCCACAGAAGGGCCACTCGAAGGTTGAATTCAAC
CTTAACGATTGTCACAAAATGATTGATTTTTTTAAGAGCTCCATTCAGAAACACCCCGAA
TGGGGGTCCTTTGGCTTCACCTTTTCTGATACTTCAGACTTCGAGGACATGTCCGCCTTC
TACAGGGAGGTGGAGAACCAGGGCTATGTCATCTCCTTCGACAAAATAAAAGAGACATAC
ATTCAGAGCCAGGTCGAGCAGGGAAATCTGTACCTGTTTCAGATCTATAACAAGGATTTC
AGTCCCTATAGCAAGGGCAAGCCCAATTTACATACCCTGTACTGGAAGGCCCTGTTCGAA
GAGGCAAACCTTAACAATGTAGTTGCTAAGCTGAATGGGGAAGCAGAGATCTTCTTCCGA
AGGCACAGCATCAAGGCAAGCGACAAAGTTGTACATCCTGCTAACCAGGCCATCGATAAC
AAGAACCCGCATACAGAAAAGACACAGTCAACCTTTGAATACGACCTCGTGAAGGACAAG
AGGTACACACAAGATAAATTCTTCTTCCACGTGCCCATCAGCTTGAATTTTAAAGCGCAG
GGAGTGAGCAAATTTAACGACAAGGTCAACGGCTTCCTGAAGGGAAACCCCGACGTGAAT
ATCATCGGAATTGATCGCGGTGAAAGACATCTCCTCTACTTTACTGTGGTGAACCAGAAG
GGTGAGATCCTAGTACAGGAGAGCCTGAACACCCTTATGAGTGATAAGGGCCATGTGAAT
GATTACCAGCAGAAGCTGGACAAGAAGGAACAGGAAAGGGACGCAGCGCGGAAGTCCTGG
ACCACTGTTGAGAATATCAAAGAACTGAAGGAGGGATATCTTAGCCATGTGGTACACAAA
CTTGCACATCTGATTATCAAGTATAATGCCATAGTCTGCCTGGAAGACTTGAACTTCGGT
TTCAAGCGAGGAAGGTTTAAAGTGGAGAAGCAGGTGTACCAGAAGTTTGAGAAAGCCCTT
ATTGATAAGCTAAACTACCTTGTCTTTAAGGAAAAAGAACTCGGCGAAGTTGGCCACTAT
TTAACCGCCTACCAACTAACCGCCCCTTTCGAGTCTTTTAAGAAACTGGGAAAGCAGAGC
GGAATACTCTTCTATGTGCCTGCAGACTACACCTCTAAGATCGACCCCACTACCGGCTTT
GTAAACTTTCTAGATCTCCGCTATCAGTCAGTAGAAAAAGCCAAACAGCTCTTGTCAGAT
TTTAACGCCATCCGATTTAATTCCGTCCAAAATTACTTCGAGTTCGAAATCGACTATAAA
AAACTTACCCCCAAGAGAAAGGTTGGGACGCAGTCTAAGTGGGTAATCTGCACTTACGGT
GACGTGAGATACCAGAACCGCCGAAACCAGAAAGGTCATTGGGAAACCGAGGAAGTGAAT
GTGACTGAGAAGCTCAAGGCCCTCTTCGCTAGCGACAGTAAAACAACAACAGTTATCGAT
TACGCCAATGACGATAATCTTATAGACGTGATCTTGGAACAAGACAAAGCCTCTTTTTTT
AAGGAATTGTTGTGGTTGCTGAAACTTACAATGACCCTTAGGCACAGCAAGATCAAATCA
GAGGATGACTTCATCCTCAGCCCGGTGAAGAATGAACAGGGAGAGTTCTACGATTCACGG
AAGGCTGGAGAGGTGTGGCCCAAGGATGCCGACGCGAACGGGGCCTACCACATAGCTCTA
AAAGGTCTGTGGAACCTGCAACAAATCAATCAATGGGAGAAAGGTAAGACACTGAACCTG
GCCATCAAAAATCAAGATTGGTTCTCATTCATCCAGGAAAAGCCTTATCAAGAGTGA
SEQ ATGCATACGGGAGGCCTTTTATCAATGGACGCAAAAGAGTTCACCGGGCAGTATCCATTA
ID TCTAAGACACTCCGCTTCGAGCTGAGGCCCATTGGCAGGACCTGGGACAACCTGGAGGCG
NO: TCGGGCTACCTGGCTGAGGACAGACATCGCGCAGAATGCTATCCGAGAGCTAAGGAGCTT
151 TTGGACGACAATCATCGCGCGTTCCTTAACCGGGTGCTCCCACAGATCGATATGGACTGG
CACCCGATCGCTGAGGCTTTTTGCAAGGTCCATAAGAACCCTGGGAACAAAGAGCTCGCC
CAGGACTACAACTTGCAGCTGAGCAAGCGACGGAAAGAGATTTCTGCCTACCTTCAAGAC
GCCGATGGCTACAAAGGGCTCTTCGCAAAGCCCGCATTGGATGAGGCCATGAAAATCGCC
AAGGAGAACGGGAATGAAAGTGACATCGAAGTTCTCGAAGCGTTTAACGGATTTAGCGTG
TACTTTACCGGCTATCATGAGTCAAGGGAGAATATTTATAGCGATGAGGACATGGTCTCT
GTGGCCTACCGGATTACCGAGGATAATTTCCCGAGGTTTGTTTCAAATGCACTAATATTC
GACAAGTTAAATGAGAGCCACCCAGACATCATCTCGGAGGTCAGCGGCAACCTCGGAGTT
GACGATATTGGCAAATACTTCGACGTGAGCAACTATAACAACTTCCTCTCACAGGCTGGC
ATCGACGACTATAATCATATTATAGGCGGCCACACTACTGAGGATGGTCTCATTCAGGCA
TTCAATGTAGTCTTGAATCTTAGGCACCAGAAGGACCCTGGGTTTGAAAAGATACAGTTC
AAGCAGCTGTATAAGCAGATATTATCCGTGCGAACATCTAAAAGTTACATCCCCAAACAG
TTTGATAACTCAAAGGAGATGGTGGATTGCATATGCGATTATGTGTCAAAAATTGAAAAG
AGCGAGACTGTGGAGCGGGCTCTGAAGCTCGTCAGGAACATTAGCTCCTTTGACCTTAGA
GGAATTTTCGTCAATAAAAAGAATCTGAGGATCCTGAGCAATAAGCTAATAGGAGATTGG
GACGCCATAGAGACAGCATTGATGCATTCCAGCTCAAGCGAGAATGATAAGAAGTCTGTC
TACGATAGCGCTGAAGCCTTCACGCTGGACGATATCTTCTCTTCCGTGAAAAAATTTAGT
GATGCGTCCGCAGAAGATATCGGGAATCGAGCCGAAGATATCTGCAGGGTAATTTCAGAG
ACCGCCCCTTTCATCAATGACCTGCGCGCCGTGGACCTGGATAGCCTGAATGACGATGGT
TACGAAGCTGCAGTTTCTAAGATCAGGGAGTCTCTGGAGCCATATATGGACTTGTTTCAC
GAACTTGAGATCTTTAGCGTGGGCGACGAGTTCCCGAAATGCGCAGCTTTCTATAGCGAG
TTAGAGGAGGTCAGCGAGCAATTAATCGAGATCATACCCCTGTTTAATAAGGCACGGAGC
TTTTGTACTCGCAAGCGCTACAGCACCGACAAGATTAAAGTTAATCTGAAATTTCCAACT
CTCGCAGACGGGTGGGACCTAAACAAGGAACGCGATAATAAAGCCGCCATCCTTAGAAAG
GACGGAAAGTACTATCTTGCCATCCTAGATATGAAAAAAGATCTGAGTTCCATTCGTACT
AGCGATGAAGACGAATCTTCTTTCGAAAAAATGGAGTATAAGCTGCTCCCCTCGCCAGTC
AAGATGCTACCCAAGATCTTTGTGAAGAGCAAAGCAGCCAAGGAAAAGTACGGGCTGACG
GACAGGATGCTGGAGTGCTACGATAAGGGAATGCATAAATCAGGGTCAGCTTTTGACTTG
GGCTTTTGCCATGAGCTAATCGATTACTACAAGCGCTGTATCGCCGAGTATCCAGGATGG
GACGTTTTCGACTTTAAATTTCGGGAGACTTCTGATTATGGTTCAATGAAGGAGTTCAAC
GAAGATGTCGCTGGTGCCGGTTACTACATGAGCCTTCGCAAGATTCCTTGTTCCGAAGTC
TACCGGCTACTGGACGAGAAATCTATATATTTGTTCCAGATATATAACAAGGACTACAGT
GAGAATGCACATGGGAATAAGAATATGCATACTATGTATTGGGAAGGTCTCTTTTCACCC
CAAAATTTGGAGTCACCCGTGTTCAAACTTAGCGGTGGCGCAGAGCTGTTCTTTAGGAAA
TCCAGTATACCCAATGACGCCAAGACAGTCCACCCAAAGGGTAGCGTCCTGGTGCCCAGA
AACGATGTGAACGGCAGGAGAATCCCTGACAGCATTTACCGAGAACTTACCAGGTACTTC
AACCGCGGCGACTGTAGAATCTCTGATGAGGCAAAGTCTTATCTGGATAAGGTGAAGACT
AAGAAGGCAGATCATGACATTGTGAAAGACCGCCGCTTTACTGTCGACAAAATGATGTTT
CACGTGCCTATCGCAATGAATTTTAAGGCAATCTCAAAACCGAATCTGAACAAGAAGGTG
ATAGATGGCATTATCGATGACCAGGACCTCAAGATCATCGGAATCGACAGAGGTGAGCGA
AACCTGATATACGTCACAATGGTAGATCGGAAGGGTAATATTCTGTACCAGGATTCACTA
AACATCCTCAATGGATATGACTATCGAAAAGCTCTCGATGTCAGGGAATACGACAACAAG
GAGGCGCGACGGAATTGGACAAAGGTGGAAGGCATACGGAAGATGAAGGAAGGCTATCTG
TCACTAGCTGTCTCCAAATTGGCTGATATGATTATAGAGAACAACGCCATTATCGTGATG
GAAGATCTCAACCATGGATTCAAGGCAGGAAGAAGTAAAATTGAGAAGCAGGTGTATCAG
AAGTTCGAAAGCATGCTTATTAATAAGTTGGGTTATATGGTCTTAAAGGACAAGTCTATC
GATCAGAGCGGCGGCGCACTCCATGGGTATCAGCTGGCTAACCATGTCACCACACTAGCA
TCCGTAGGCAAACAGTGTGGCGTGATTTTCTACATTCCTGCTGCGTTCACTTCTAAGATC
GATCCTACCACGGGATTCGCAGACCTGTTCGCACTGAGCAATGTTAAAAACGTGGCCTCC
ATGAGGGAGTTCTTTAGCAAAATGAAAAGCGTGATTTATGACAAGGCCGAGGGCAAGTTC
GCTTTCACATTTGACTACCTGGACTACAATGTGAAATCAGAGTGCGGGAGAACCCTGTGG
ACCGTATACACGGTAGGGGAAAGATTCACTTACAGTCGAGTTAATCGGGAGTATGTCCGT
AAAGTGCCAACTGACATCATCTACGATGCCCTTCAGAAGGCTGGCATAAGTGTTGAGGGG
GATCTAAGGGACAGGATCGCTGAATCGGATGGCGATACTCTCAAATCAATCTTCTACGCC
TTCAAGTATGCCCTCGACATGAGGGTAGAGAACCGGGAGGAGGACTATATACAGTCTCCC
GTGAAGAATGCGTCGGGAGAGTTCTTCTGCTCAAAAAACGCCGGGAAATCTTTGCCGCAG
GATTCTGATGCAAATGGGGCTTATAACATTGCTCTCAAAGGCATCCTGCAGCTGCGCATG
CTATCTGAACAATATGACCCAAACGCTGAAAGCATTAGATTGCCATTGATCACCAATAAG
GCTTGGCTGACTTTCATGCAGAGCGGTATGAAGACATGGAAAAACTAA
SEQ ATGGATTCCCTTAAGGACTTCACAAATCTTTACCCCGTGAGTAAAACCCTGAGATTTGAA
ID CTCAAGCCCGTGGGAAAGACTCTCGAGAATATCGAGAAGGCCGGGATTTTGAAGGAAGAC
NO: GAGCATCGGGCGGAAAGTTACAGACGGGTGAAGAAGATTATAGATACTTATCACAAGGTC
152 TTTATAGACAGCTCTTTAGAGAACATGGCAAAGATGGGCATCGAGAACGAAATCAAGGCC
ATGCTGCAGTCCTTCTGCGAGCTGTATAAAAAGGATCATCGGACCGAAGGCGAAGACAAG
GCGCTGGATAAGATCAGGGCAGTGCTGCGCGGCCTCATTGTGGGTGCCTTCACTGGGGTG
TGCGGGCGGAGAGAGAACACTGTGCAGAATGAGAAATACGAGAGTTTGTTCAAAGAGAAA
CTCATCAAGGAAATCCTGCCCGACTTCGTCTTAAGCACAGAAGCCGAATCTCTCCCATTT
TCTGTCGAGGAGGCCACGCGTTCCCTTAAAGAGTTCGACAGTTTCACTTCATACTTTGCC
GGATTTTATGAAAACCGTAAAAATATATACTCCACTAAACCACAGTCAACTGCAATAGCT
TACAGGTTAATCCACGAAAACCTGCCAAAATTCATCGACAATATACTCGTCTTTCAAAAA
ATCAAGGAACCAATCGCGAAGGAACTTGAACACATCCGGGCTGACTTTAGTGCGGGAGGA
TACATCAAAAAAGACGAGCGCCTGGAGGATATATTTTCACTAAATTATTATATTCATGTA
CTGAGCCAGGCTGGCATAGAAAAGTACAACGCTCTAATTGGGAAAATCGTGACAGAAGGT
GACGGGGAAATGAAAGGGCTAAACGAACATATTAACTTATATAACCAACAGCGGGGTCGA
GAAGATCGTCTGCCCCTGTTCAGACCTCTGTATAAGCAAATACTCTCCGACAGAGAGCAG
CTATCATATCTGCCCGAGTCCTTTGAGAAAGATGAAGAGCTGCTCCGGGCGCTCAAGGAG
TTCTATGATCATATAGCCGAGGACATTTTGGGCAGAACTCAGCAACTCATGACGTCTATT
TCTGAATATGATCTGTCTCGTATCTATGTCAGGAATGATAGCCAGCTGACCGATATATCC
AAGAAGATGCTGGGGGACTGGAACGCCATTTATATGGCGAGGGAGCGAGCATACGATCAC
GAGCAGGCACCCAAGAGAATCACAGCCAAATATGAGAGAGACCGCATTAAGGCGCTGAAG
GGCGAAGAAAGTATCAGTCTGGCCAATCTGAACTCCTGCATAGCTTTCCTTGATAACGTG
AGGGATTGCAGAGTTGATACTTACCTGAGTACCCTGGGCCAGAAGGAAGGGCCTCACGGC
CTCTCTAATCTAGTGGAGAATGTATTTGCCTCCTACCACGAAGCTGAGCAGCTGCTGTCA
TTTCCGTACCCAGAGGAAAATAATTTAATACAGGATAAGGACAACGTAGTGCTTATCAAA
AATCTACTGGATAACATTTCCGACCTCCAGCGCTTTCTCAAACCACTTTGGGGGATGGGC
GACGAGCCTGATAAGGATGAGCGCTTTTACGGCGAGTACAACTACATCAGGGGCGCCTTG
GACCAGGTGATTCCCCTCTATAATAAAGTCAGGAATTACCTGACCCGAAAGCCATACAGT
ACAAGAAAGGTGAAATTAAATTTCGGCAATAGTCAGCTGCTGTCTGGTTGGGACCGAAAT
AAGGAGAAAGACAACAGCTGCGTAATTCTCAGAAAAGGACAGAACTTTTATTTGGCCATC
ATGAATAACAGACACAAGAGATCTTTCGAGAACAAAGTGCTCCCTGAGTATAAGGAGGGG
GAACCCTACTTCGAGAAGATGGACTATAAATTCCTTCCTGATCCAAATAAAATGCTGCCT
AAAGTATTTCTGTCAAAAAAAGGTATAGAAATCTACAAACCTTCACCTAAGCTACTTGAA
CAGTATGGCCACGGCACCCATAAAAAAGGGGACACGTTCAGCATGGACGACCTACACGAA
CTGATTGACTTCTTTAAGCACAGCATAGAAGCTCATGAGGACTGGAAACAGTTCGGATTC
AAATTCTCAGATACCGCGACCTACGAAAACGTGTCTAGTTTTTACCGGGAAGTCGAGGAC
CAGGGCTACAAGCTCAGCTTCAGAAAAGTTAGCGAATCTTACGTCTACTCCCTTATAGAT
CAAGGTAAGCTGTATCTCTTTCAAATCTACAACAAGGACTTTTCCCCATGTAGCAAGGGC
ACCCCCAATCTGCACACTCTCTACTGGCGGATGCTGTTCGACGAGCGTAACCTGGCAGAC
GTGATCTACAAATTAGATGGTAAAGCTGAGATCTTCTTTCGTGAAAAGAGCCTAAAGAAC
GATCACCCCACTCACCCCGCCGGAAAGCCCATTAAGAAGAAAAGTAGGCAGAAGAAAGGA
GAAGAATCGCTATTTGAGTACGACCTCGTCAAGGATCGGCATTATACAATGGATAAGTTC
CAGTTCCATGTGCCAATAACTATGAATTTCAAGTGCAGTGCTGGCAGTAAGGTGAATGAC
ATGGTAAACGCTCATATCCGGGAGGCAAAGGACATGCATGTTATTGGAATTGATAGGGGT
GAGCGTAATCTCCTCTACATCTGTGTTATTGACTCCCGCGGCACAATCCTCGATCAGATT
TCCTTGAATACAATTAATGATATAGACTACCATGACTTGCTTGAGTCTCGCGACAAAGAT
AGACAGCAGGAGAGAAGAAATTGGCAGACCATCGAAGGCATCAAGGAACTCAAGCAAGGC
TACCTTTCTCAGGCAGTGCATCGAATAGCCGAGCTGATGGTGGCTTATAAAGCCGTCGTG
GCACTAGAAGACCTAAATATGGGATTTAAACGAGGCAGGCAGAAGGTGGAATCATCCGTA
TACCAGCAGTTCGAAAAACAGTTGATAGACAAACTCAATTACCTTGTAGACAAGAAGAAG
CGGCCTGAGGACATAGGGGGCCTGCTTAGAGCGTATCAATTTACAGCCCCATTCAAGTCT
TTCAAAGAAATGGGTAAACAGAACGGTTTTCTGTTTTACATCCCAGCGTGGAACACCAGC
AATATAGATCCAACCACTGGCTTCGTCAATCTGTTTCATGCTCAGTATGAAAATGTGGAC
AAGGCCAAATCCTTCTTTCAGAAATTTGACAGCATCTCCTATAACCCAAAGAAAGACTGG
TTTGAATTCGCCTTTGACTATAAGAATTTCACTAAGAAGGCCGAGGGATCAAGAAGCATG
TGGATATTGTGCACGCATGGCTCACGTATAAAGAACTTTAGAAACTCGCAAAAAAACGGG
CAGTGGGACTCAGAAGAATTCGCACTCACCGAGGCTTTCAAATCCCTCTTCGTCCGGTAT
GAGATCGATTACACCGCCGATCTGAAGACGGCAATCGTCGACGAGAAACAGAAAGACTTC
TTTGTAGATCTACTTAAGCTCTTTAAGCTAACCGTTCAGATGCGAAACAGTTGGAAAGAA
AAGGATCTCGACTATCTCATTAGTCCAGTGGCTGGCGCGGATGGTAGATTTTTCGATACC
CGGGAAGGTAACAAGTCCCTTCCCAAAGACGCCGACGCGAATGGTGCCTACAATATTGCA
CTAAAGGGGCTCTGGGCGCTGCGGCAAATTAGACAGACATCTGAAGGGGGCAAGCTTAAG
CTGGCTATTTCTAATAAAGAGTGGTTGCAGTTTGTGCAGGAAAGGAGTTATGAGAAGGAC
TAG
SEQ ATGAACAACGGCACCAACAACTTCCAGAACTTCATCGGCATATCGTCTCTGCAGAAAACA
ID CTTAGGAATGCCCTGATTCCAACTGAGACAACACAGCAGTTTATTGTGAAGAATGGGATC
NO: ATCAAAGAGGACGAATTGCGCGGGGAGAATAGGCAGATCCTGAAGGACATCATGGACGAT
153 TACTACAGGGGTTTTATCTCCGAAACGCTGAGCTCGATTGACGATATTGACTGGACGTCC
CTCTTTGAGAAGATGGAAATCCAACTTAAAAATGGCGATAATAAAGATACCCTGATAAAG
GAACAAACCGAATATAGAAAGGCTATACACAAAAAATTCGCAAATGACGACCGCTTTAAG
AACATGTTTTCTGCAAAACTGATTAGCGATATTCTGCCCGAGTTTGTGATTCACAATAAT
AACTATTCCGCTTCGGAGAAGGAGGAAAAGACTCAGGTGATTAAACTGTTTTCTCGGTTC
GCCACTTCTTTCAAAGATTATTTCAAAAATCGCGCCAACTGTTTTTCCGCTGACGACATC
TCCTCCTCTTCCTGCCACCGGATCGTAAACGACAATGCCGAGATCTTTTTTAGTAACGCC
CTTGTGTATCGGAGGATAGTGAAGAGCCTGTCCAATGATGACATAAACAAAATTTCTGGC
GATATGAAGGATAGCCTCAAAGAGATGAGCCTTGAAGAAATTTACTCCTACGAGAAGTAT
GGGGAGTTCATCACCCAGGAGGGGATTTCCTTCTATAATGACATCTGTGGCAAGGTGAAC
AGCTTCATGAACCTGTACTGCCAGAAGAATAAGGAAAACAAAAATCTGTACAAGCTTCAG
AAGTTACATAAGCAGATCCTGTGTATCGCGGATACCTCATATGAGGTTCCTTATAAGTTC
GAGAGTGATGAAGAAGTGTACCAGTCTGTAAATGGATTCTTAGACAATATTTCGTCCAAA
CATATAGTGGAGAGACTGAGAAAGATCGGGGACAATTACAATGGGTACAATCTCGACAAG
ATTTATATCGTGTCGAAGTTTTACGAATCTGTGAGCCAGAAAACATACAGGGATTGGGAA
ACCATTAATACCGCGCTTGAAATTCACTACAATAATATTCTGCCTGGCAACGGAAAAAGC
AAGGCCGATAAGGTAAAAAAGGCAGTCAAAAATGACCTTCAGAAAAGTATCACCGAAATC
AATGAGTTGGTGAGCAACTACAAATTGTGTTCAGACGATAATATTAAAGCGGAAACGTAC
ATACATGAAATTAGCCATATTCTGAATAACTTTGAGGCGCAGGAACTTAAGTACAACCCT
GAAATTCATCTCGTCGAAAGCGAATTGAAGGCCTCTGAATTGAAAAACGTTCTTGACGTG
ATAATGAACGCTTTCCATTGGTGCTCTGTGTTTATGACTGAAGAGCTGGTTGATAAGGAC
AACAACTTTTATGCTGAACTTGAGGAAATCTACGACGAGATCTACCCTGTGATTAGCTTG
TATAACCTCGTCAGAAACTACGTTACCCAGAAGCCGTACAGCACGAAAAAAATAAAGCTG
AACTTTGGTATTCCGACTCTCGCCGATGGATGGAGCAAGTCGAAGGAATATTCCAACAAT
GCCATCATTCTTATGCGAGACAATCTGTATTACCTCGGCATCTTTAACGCCAAAAACAAG
CCGGATAAGAAAATCATTGAAGGGAATACGAGCGAGAATAAGGGCGACTATAAGAAAATG
ATCTACAACTTACTGCCAGGTCCCAATAAAATGATTCCTAAGGTGTTTCTGTCATCGAAA
ACAGGTGTAGAAACATATAAGCCCAGCGCATACATCCTGGAAGGCTACAAGCAAAACAAA
CACATCAAAAGCAGCAAGGACTTTGATATCACATTCTGCCACGATCTAATCGACTACTTC
AAAAATTGCATCGCCATTCACCCTGAGTGGAAGAACTTCGGCTTTGACTTCTCCGACACC
AGTACCTACGAAGACATTTCTGGATTCTACCGTGAGGTTGAGCTGCAGGGTTATAAAATT
GACTGGACATACATCAGTGAAAAAGACATCGATCTACTGCAGGAGAAGGGGCAGCTCTAT
CTCTTCCAGATTTATAATAAGGATTTCAGCAAGAAGTCCACTGGAAACGACAATCTGCAT
ACAATGTATCTTAAGAACTTGTTTAGCGAAGAGAATTTGAAAGATATCGTTCTAAAGTTA
AACGGGGAAGCCGAGATTTTCTTTCGAAAGTCTTCCATTAAGAATCCAATTATTCACAAG
AAGGGCAGTATCCTGGTCAACAGAACCTATGAGGCCGAGGAAAAGGACCAGTTCGGTAAT
ATACAAATTGTGCGCAAGAACATCCCCGAGAACATTTACCAGGAGCTCTATAAATACTTC
AACGACAAAAGCGATAAGGAGCTTTCCGACGAGGCTGCCAAGCTGAAAAACGTGGTGGGA
CACCATGAAGCAGCCACCAACATCGTCAAAGATTATCGTTATACATATGACAAATATTTT
CTGCACATGCCTATTACAATAAACTTTAAGGCAAACAAGACCGGGTTCATCAATGACCGG
ATACTCCAGTACATCGCAAAAGAGAAGGACCTGCATGTGATCGGCATCGACCGCGGTGAA
AGAAATCTCATTTACGTCAGCGTTATCGACACTTGTGGAAACATTGTGGAGCAGAAGTCC
TTCAACATTGTTAACGGCTATGACTATCAGATCAAGCTCAAACAGCAGGAAGGTGCTCGT
CAGATTGCGAGGAAAGAATGGAAAGAGATCGGCAAGATCAAGGAGATCAAAGAAGGGTAT
CTGAGCTTGGTCATTCACGAGATCTCCAAAATGGTCATCAAGTACAACGCTATTATCGCG
ATGGAAGACCTCTCTTACGGCTTTAAGAAGGGGCGCTTTAAAGTGGAGCGCCAGGTCTAT
CAGAAGTTCGAGACTATGCTTATCAATAAGCTGAATTACTTGGTCTTTAAGGATATCAGT
ATCACCGAGAACGGAGGACTGCTGAAAGGTTACCAGCTCACATATATTCCCGATAAGCTC
AAGAATGTGGGCCACCAATGCGGTTGTATTTTTTACGTTCCAGCTGCCTACACATCTAAG
ATCGATCCTACCACCGGATTCGTCAATATATTTAAATTTAAAGATCTAACCGTTGATGCC
AAGCGTGAGTTTATTAAGAAATTTGATTCAATCAGGTACGACAGCGAAAAGAACCTCTTC
TGTTTCACTTTCGACTACAACAACTTCATCACACAAAATACTGTGATGAGCAAGTCATCA
TGGAGCGTTTATACTTATGGTGTAAGGATAAAAAGGCGCTTTGTTAATGGAAGGTTTTCC
AATGAAAGCGATACAATAGACATCACAAAAGACATGGAGAAGACACTGGAGATGACAGAT
ATTAATTGGAGGGACGGGCATGACCTTAGACAGGACATCATCGACTACGAAATCGTCCAA
CACATTTTTGAGATATTCAGACTCACTGTCCAGATGCGAAACAGCCTGTCGGAACTCGAA
GACCGGGACTACGATAGACTGATCTCCCCGGTGTTAAACGAAAATAATATTTTCTACGAT
TCTGCTAAGGCAGGAGACGCTCTTCCTAAAGATGCGGACGCCAATGGCGCTTACTGTATA
GCGTTGAAGGGATTGTATGAGATTAAACAGATCACTGAGAATTGGAAAGAAGACGGTAAA
TTCTCCAGAGACAAGCTGAAAATCTCCAACAAAGACTGGTTTGATTTTATTCAAAATAAG
CGCTACCTGTAA
SEQ ATGACAAACAAATTTACTAATCAGTACAGCCTGTCAAAGACCCTCCGCTTCGAACTGATT
ID CCACAAGGGAAGACCCTTGAATTCATCCAGGAAAAGGGTTTATTATCCCAGGATAAACAA
NO: CGCGCAGAAAGCTATCAAGAGATGAAGAAGACGATCGATAAATTTCATAAGTATTTCATA
154 GATTTAGCCCTGAGCAACGCTAAATTGACCCACCTGGAAACCTATTTGGAGCTGTACAAC
AAGTCAGCCGAGACAAAGAAAGAGCAGAAGTTTAAGGACGACCTGAAAAAAGTACAGGAC
AATTTGCGAAAAGAGATCGTCAAGTCTTTTTCCGACGGAGACGCCAAGTCAATATTTGCC
ATCCTGGACAAAAAGGAACTCATCACTGTGGAGTTGGAGAAGTGGTTTGAGAATAATGAG
CAGAAGGACATCTATTTTGACGAAAAGTTCAAGACATTTACTACTTACTTCACCGGATTT
CACCAAAACCGGAAGAACATGTACTCTGTTGAGCCGAACTCAACCGCCATCGCCTACCGC
CTTATTCACGAAAATCTGCCAAAGTTTCTCGAGAATGCTAAAGCCTTTGAGAAAATTAAG
CAGGTCGAGTCGCTCCAGGTGAACTTTCGAGAGCTGATGGGTGAATTCGGGGACGAGGGC
CTGATTTTCGTGAATGAACTCGAAGAGATGTTTCAGATCAACTACTATAATGATGTACTC
TCACAGAACGGGATCACTATCTACAACAGCATTATCTCTGGATTCACTAAGAACGATATC
AAGTATAAAGGGCTGAATGAATACATCAACAATTATAATCAGACTAAGGACAAAAAGGAC
AGGCTGCCTAAATTGAAACAGCTGTATAAGCAGATCCTCAGTGATAGAATTAGCTTGTCA
TTTCTCCCAGATGCCTTCACTGACGGAAAGCAGGTGCTTAAGGCGATATTCGATTTCTAT
AAGATCAACCTCCTCTCTTATACAATCGAGGGCCAGGAGGAGTCACAGAACCTCCTGCTC
CTGATTCGACAAACTATTGAAAATCTGTCCTCTTTCGATACGCAGAAGATATACCTGAAA
AATGACACCCATCTCACTACAATATCCCAACAGGTATTCGGAGATTTCTCCGTCTTCAGT
ACAGCCCTGAATTACTGGTACGAGACAAAGGTGAACCCTAAGTTCGAAACAGAGTACAGC
AAGGCGAACGAAAAGAAGAGGGAGATCCTGGACAAAGCCAAAGCCGTTTTCACCAAGCAA
GATTACTTTAGCATCGCATTTCTGCAGGAAGTCCTGTCTGAGTACATACTGACACTCGAT
CACACAAGCGACATAGTTAAGAAGCACTCTTCCAATTGTATCGCGGACTACTTCAAAAAT
CATTTTGTCGCGAAAAAGGAGAACGAGACAGATAAGACCTTCGATTTTATCGCGAATATT
ACCGCAAAGTATCAATGCATTCAGGGTATCTTGGAGAACGCCGACCAGTACGAAGACGAG
CTTAAACAGGATCAGAAGCTCATCGACAACCTAAAGTTCTTTTTGGACGCTATACTGGAA
CTCCTTCATTTTATTAAGCCACTACATCTGAAGAGTGAGTCTATCACTGAGAAGGACACT
GCTTTTTACGACGTTTTCGAGAATTACTACGAAGCACTGTCTCTGCTAACCCCTCTGTAT
AACATGGTGAGAAACTATGTGACACAGAAACCTTATAGTACCGAGAAGATTAAGTTGAAC
TTCGAGAACGCACAATTGCTGAATGGGTGGGATGCAAACAAAGAGGGTGATTACCTCACA
ACAATCCTCAAGAAAGATGGCAATTACTTCCTGGCCATTATGGATAAAAAACATAACAAG
GCATTTCAGAAATTTCCCGAGGGGAAGGAAAATTATGAAAAGATGGTATACAAGTTGCTG
CCCGGGGTGAACAAAATGCTCCCGAAGGTGTTTTTCTCGAATAAGAATATCGCGTACTTT
AACCCGTCCAAGGAACTGTTGGAAAATTATAAAAAGGAAACACACAAGAAGGGGGACACT
TTTAATTTGGAGCACTGCCACACACTCATTGACTTCTTTAAAGATAGTCTCAACAAACAT
GAGGATTGGAAATATTTTGACTTTCAGTTTAGCGAGACCAAGTCTTATCAGGATCTGTCG
GGATTTTATAGGGAAGTTGAGCACCAGGGTTACAAGATAAATTTCAAGAACATCGATAGC
GAGTACATTGACGGACTGGTGAACGAAGGGAAGCTGTTCCTGTTTCAGATTTACAGCAAA
GATTTCTCTCCTTTCTCAAAAGGCAAGCCGAACATGCATACCCTGTATTGGAAGGCCCTG
TTCGAGGAGCAAAACCTTCAGAATGTGATTTACAAGCTGAACGGTCAGGCCGAGATTTTT
TTTAGGAAGGCCTCTATCAAGCCCAAAAACATCATTCTGCACAAGAAAAAGATAAAGATC
GCCAAAAAACACTTCATTGATAAAAAGACAAAGACTTCTGAGATCGTACCTGTTCAGACA
ATCAAGAATCTCAACATGTATTATCAGGGGAAGATTAGCGAGAAAGAGCTGACACAGGAC
GATTTGAGGTACATCGACAACTTCTCTATCTTTAACGAGAAGAACAAGACAATCGATATC
ATCAAGGACAAGCGGTTTACCGTCGATAAATTCCAGTTCCATGTGCCTATCACGATGAAT
TTCAAGGCCACCGGTGGGAGTTATATCAACCAGACTGTGCTGGAGTATCTGCAGAACAAC
CCCGAAGTAAAAATTATTGGCCTGGACAGAGGAGAGCGGCATCTGGTGTACTTGACCCTC
ATCGATCAGCAGGGAAATATCCTGAAACAAGAATCTCTGAATACTATTACGGACTCCAAA
ATCAGCACACCTTACCACAAGCTGCTTGATAATAAAGAGAATGAGAGGGACTTGGCCCGC
AAAAATTGGGGCACCGTCGAGAATATTAAGGAATTGAAAGAAGGATACATCTCACAGGTG
GTTCACAAAATCGCAACCCTGATGTTAGAAGAGAACGCTATTGTGGTGATGGAGGACTTA
AACTTCGGATTTAAAAGAGGAAGATTTAAAGTCGAGAAACAGATTTATCAGAAACTGGAA
AAAATGCTCATTGACAAATTAAATTACCTGGTGCTGAAAGATAAACAGCCACAGGAGCTG
GGTGGCCTGTATAATGCTCTGCAGCTGACCAACAAGTTCGAGTCGTTTCAGAAAATGGGC
AAGCAGTCAGGCTTCCTTTTTTACGTGCCCGCTTGGAACACCTCAAAAATCGACCCTACA
ACAGGCTTTGTGAATTATTTCTATACCAAGTATGAAAACGTGGACAAGGCAAAGGCCTTT
TTCGAGAAGTTTGAAGCAATCAGGTTCAATGCCGAGAAAAAATACTTTGAGTTCGAGGTC
AAAAAATATAGCGACTTCAACCCTAAGGCCGAAGGCACGCAACAAGCCTGGACAATATGC
ACGTATGGGGAGAGAATTGAGACTAAGCGGCAGAAGGATCAGAATAACAAATTCGTGAGC
ACACCGATTAACCTGACAGAGAAGATAGAGGACTTCCTCGGCAAGAATCAGATCGTGTAC
GGCGACGGCAATTGCATCAAGTCACAAATTGCATCTAAAGATGACAAAGCATTCTTCGAA
ACACTGCTGTATTGGTTCAAGATGACACTCCAGATGCGAAATAGCGAAACAAGAACAGAT
ATTGACTACCTCATCAGCCCTGTGATGAATGATAACGGCACGTTTTACAATTCCCGGGAC
TATGAAAAATTAGAGAACCCGACACTGCCAAAAGACGCCGACGCAAATGGTGCATATCAC
ATCGCAAAGAAAGGTTTGATGCTGTTGAACAAAATTGATCAGGCTGATCTGACAAAAAAG
GTCGATCTGAGTATCAGTAACCGCGACTGGTTGCAGTTTGTCCAGAAGAACAAATAA
SEQ ATGGAACAAGAGTACTATCTGGGCCTGGACATGGGCACCGGGAGTGTCGGATGGGCAGTC
ID ACCGACTCAGAGTACCACGTCCTCAGAAAGCACGGTAAGGCACTTTGGGGAGTGCGACTC
NO: TTCGAGTCCGCTAGTACTGCTGAAGAGAGGAGGATGTTTCGAACTTCCAGGCGCAGGCTG
155 GATCGGCGAAACTGGAGAATAGAGATTCTCCAGGAGATATTTGCTGAAGAGATTTCAAAG
AAGGATCCTGGTTTTTTCCTGCGCATGAAAGAATCTAAGTATTACCCCGAAGATAAACGC
GACATCAACGGCAATTGTCCTGAACTGCCCTATGCTCTGTTTGTCGACGACGATTTCACC
GACAAAGATTACCACAAGAAATTCCCCACCATATACCACCTGAGAAAGATGTTGATGAAC
ACCGAGGAGACACCCGACATACGTCTGGTTTACCTGGCTATCCATCATATGATGAAGCAC
CGCGGGCATTTCCTGCTGTCTGGAGACATCAATGAGATAAAGGAATTTGGTACTACGTTC
TCCAAGTTGTTAGAAAACATTAAGAATGAAGAGTTGGACTGGAATCTTGAACTGGGAAAG
GAAGAGTATGCAGTTGTAGAGTCGATTTTGAAAGATAACATGTTAAACCGGTCAACTAAG
AAAACCAGGTTAATTAAGGCACTAAAGGCCAAATCGATATGCGAGAAGGCTGTGCTAAAT
CTGCTGGCTGGAGGCACCGTGAAACTGTCTGATATTTTCGGCCTGGAAGAGCTCAATGAA
ACCGAGCGGCCTAAAATTTCTTTCGCCGATAACGGATACGATGACTATATTGGGGAGGTG
GAAAACGAGCTCGGAGAACAATTCTACATTATTGAAACCGCTAAGGCAGTCTATGACTGG
GCCGTGCTCGTCGAGATTTTAGGCAAGTACACCAGCATTAGCGAAGCAAAGGTGGCTACC
TATGAAAAGCACAAATCTGACCTCCAGTTTCTGAAAAAGATTGTGCGCAAATACTTAACA
AAAGAAGAGTACAAGGACATCTTTGTGAGCACATCAGATAAGCTCAAGAATTACTCAGCA
TACATTGGAATGACAAAGATTAACGGGAAGAAGGTGGATCTCCAAAGCAAACGTTGTTCA
AAGGAGGAGTTTTACGATTTCATAAAGAAGAACGTGCTGAAGAAACTGGAGGGACAACCG
GAGTACGAGTATTTAAAGGAGGAGCTCGAGCGAGAAACTTTCCTGCCCAAGCAAGTGAAC
AGAGACAATGGTGTCATTCCTTACCAGATTCACTTATATGAGCTGAAGAAAATCCTGGGG
AACTTGAGAGACAAGATAGACCTCATCAAGGAAAATGAAGATAAGTTGGTCCAGTTGTTC
GAATTCAGAATCCCATATTACGTCGGCCCGCTCAATAAGATCGACGACGGCAAGGAAGGC
AAATTCACTTGGGCGGTGCGAAAAAGCAACGAAAAAATATACCCATGGAACTTTGAGAAC
GTCGTTGACATCGAGGCCAGCGCCGAGAAATTTATAAGACGCATGACTAATAAGTGTACT
TACCTCATGGGCGAGGATGTTCTGCCCAAGGACAGCCTGCTGTATTCCAAGTACATGGTG
CTTAACGAGCTGAATAATGTAAAGTTAGATGGTGAGAAGCTCAGCGTGGAGCTTAAACAG
AGGCTGTACACTGATGTGTTTTGCAAGTATCGGAAAGTTACCGTTAAGAAGATAAAGAAT
TACCTGAAATGCGAAGGGATCATTTCCGGCAACGTGGAAATTACCGGAATCGACGGCGAT
TTTAAGGCGTCGTTGACCGCTTATCATGATTTCAAGGAGATTTTAACCGGCACGGAGCTC
GCGAAGAAAGACAAGGAGAACATAATCACGAATATAGTTCTGTTTGGGGACGATAAAAAA
CTTCTTAAAAAACGACTCAATCGACTGTATCCGCAGATTACCCCCAACCAGCTGAAGAAG
ATTTGCGCTCTGAGCTATACCGGGTGGGGCCGGTTCTCTAAGAAATTCCTCGAGGAGATC
ACAGCACCAGACCCAGAGACTGGTGAGGTGTGGAATATTATTACAGCTCTGTGGGAATCC
AATAATAACCTTATGCAATTGTTGAGCAATGAATATAGGTTCATGGAGGAAGTGGAAACC
TACAATATGGGCAAGCAGACAAAGACCCTATCTTACGAGACCGTTGAGAATATGTATGTC
TCCCCTTCAGTGAAACGGCAAATCTGGCAAACTTTGAAGATCGTGAAGGAGCTCGAAAAG
GTGATGAAAGAGAGCCCGAAGAGGGTTTTTATTGAAATGGCCAGAGAGAAACAGGAGAGC
AAGAGAACAGAGTCTAGGAAGAAGCAGCTAATCGATTTGTATAAAGCCTGCAAGAACGAG
GAAAAAGACTGGGTCAAGGAGCTAGGCGATCAGGAAGAACAGAAGTTGCGCTCTGATAAG
CTGTACTTATATTATACCCAGAAAGGACGGTGCATGTACTCAGGTGAGGTCATTGAGCTG
AAAGATCTGTGGGACAATACTAAGTATGATATTGATCACATCTACCCTCAGTCAAAAACT
ATGGACGACTCCCTCAACAACAGGGTGTTGGTTAAGAAGAAATACAATGCTACAAAGTCC
GATAAATACCCTCTTAACGAAAACATCCGGCACGAAAGAAAGGGCTTCTGGAAGTCCCTG
CTGGATGGGGGTTTTATCAGTAAAGAAAAGTATGAGAGGCTGATCCGAAATACCGAGCTC
TCCCCCGAGGAACTGGCTGGCTTTATCGAAAGGCAGATCGTAGAGACTAGGCAATCTACA
AAGGCAGTCGCTGAGATCCTGAAGCAAGTGTTTCCTGAGTCAGAAATCGTGTACGTCAAA
GCTGGCACAGTGTCACGGTTCCGAAAGGACTTTGAGTTGTTAAAAGTTCGGGAGGTGAAT
GACCTGCACCACGCTAAAGACGCCTATCTGAATATCGTTGTGGGGAACTCCTATTATGTT
AAGTTTACTAAGAATGCGTCCTGGTTTATTAAGGAGAACCCGGGGCGCACCTATAACCTG
AAGAAGATGTTCACCTCCGGCTGGAACATAGAACGGAACGGAGAAGTCGCGTGGGAGGTG
GGTAAGAAAGGGACCATTGTGACCGTCAAACAGATTATGAACAAAAACAACATATTGGTA
ACTCGCCAGGTGCATGAGGCCAAAGGGGGCCTCTTTGATCAGCAGATTATGAAAAAGGGC
AAAGGACAGATCGCAATCAAGGAAACCGACGAGCGCCTGGCATCCATTGAGAAGTACGGA
GGCTACAACAAGGCGGCAGGTGCGTACTTCATGCTCGTCGAGTCCAAAGATAAGAAAGGC
AAAACTATTAGAACAATCGAGTTCATCCCTCTATATTTGAAAAATAAGATCGAAAGTGAC
GAAAGCATCGCCCTTAACTTCTTGGAGAAGGGCCGGGGCTTAAAGGAACCAAAGATTCTG
CTCAAGAAGATCAAGATCGACACACTCTTCGATGTGGATGGTTTTAAGATGTGGCTGTCA
GGCAGGACAGGGGATCGCTTGCTGTTCAAATGCGCAAATCAGTTGATTCTGGACGAAAAG
ATCATTGTGACGATGAAGAAGATCGTTAAATTCATTCAGCGGAGACAGGAAAACAGAGAA
CTGAAACTCTCCGATAAGGATGGAATTGACAATGAAGTCCTCATGGAGATTTACAATACC
TTTGTGGACAAGCTTGAGAACACAGTCTATCGGATCCGACTGTCCGAACAGGCAAAGACT
CTGATCGACAAACAGAAAGAATTCGAAAGACTAAGCTTAGAGGACAAAAGTTCAACTCTC
TTTGAAATTCTCCACATCTTCCAATGTCAAAGTAGTGCAGCCAACTTGAAGATGATCGGG
GGTCCCGGCAAGGCTGGAATCTTAGTCATGAACAACAACATCTCCAAATGTAACAAAATC
TCCATCATAAACCAGTCTCCCACCGGCATTTTCGAGAACGAAATTGATTTACTCAAG
SEQ ATGAAATCTTTCGATTCTTTCACCAACCTCTACTCCCTTAGCAAAACCCTTAAGTTTGAA
ID ATGAGGCCGGTGGGGAATACACAGAAGATGCTTGACAATGCTGGCGTCTTTGAAAAGGAC
NO: AAATTAATCCAGAAGAAGTATGGTAAAACAAAGCCATATTTTGACCGATTGCATCGGGAA
156 TTCATTGAAGAGGCTCTTACAGGAGTAGAATTGATCGGACTGGACGAGAACTTCCGTACC
TTAGTAGACTGGCAGAAGGACAAGAAGAACAACGTGGCAATGAAGGCCTATGAGAACTCA
CTCCAGCGCCTTAGAACCGAGATCGGAAAGATCTTTAATCTTAAGGCGGAAGATTGGGTA
AAAAATAAGTACCCGATCCTGGGACTGAAAAACAAAAACACAGACATCCTGTTTGAAGAA
GCCGTCTTTGGTATCTTGAAGGCCAGGTATGGAGAGGAGAAAGACACGTTTATAGAGGTA
GAGGAGATTGATAAAACAGGCAAGAGTAAGATTAATCAGATCAGTATCTTTGATTCTTGG
AAGGGGTTCACAGGCTACTTTAAGAAGTTTTTCGAAACCAGGAAAAATTTCTATAAGAAC
GATGGCACCTCCACAGCTATCGCGACACGCATCATAGATCAGAATCTGAAACGGTTCATT
GATAATCTGAGCATTGTTGAATCCGTGCGCCAGAAGGTCGACCTAGCTGAGACTGAGAAG
TCTTTCTCTATATCACTCTCCCAGTTCTTCTCAATAGATTTTTATAATAAGTGCCTTCTG
CAAGATGGCATAGACTACTATAACAAGATCATCGGCGGCGAAACTCTCAAAAACGGTGAA
AAGCTCATTGGCCTGAATGAGCTCATCAACCAATATAGACAAAATAACAAGGATCAGAAA
ATCCCATTCTTTAAGCTGCTAGATAAACAGATCCTATCAGAAAAAATCCTGTTCCTCGAC
GAAATCAAAAACGACACCGAACTCATCGAGGCTCTCTCGCAGTTTGCCAAGACGGCTGAG
GAGAAGACGAAGATTGTGAAAAAGCTGTTTGCAGACTTTGTGGAGAACAACTCTAAATAC
GATTTGGCTCAGATTTATATCTCCCAGGAAGCATTTAACACAATCTCCAATAAGTGGACT
AGCGAGACTGAAACCTTCGCCAAATACCTGTTCGAGGCCATGAAAAGCGGCAAGCTCGCC
AAATACGAGAAGAAGGACAATTCCTATAAGTTTCCCGATTTCATCGCATTATCTCAGATG
AAGTCCGCGCTACTTAGCATTAGCCTGGAAGGCCATTTTTGGAAGGAGAAATACTATAAG
ATTTCCAAATTCCAAGAAAAGACCAATTGGGAGCAGTTCTTGGCTATTTTTCTATACGAG
TTCAACTCTTTGTTCAGTGACAAGATCAACACTAAGGACGGTGAGACCAAACAAGTGGGG
TACTACCTCTTCGCCAAAGATCTTCATAACCTGATACTGTCCGAACAGATCGACATACCC
AAGGATTCAAAGGTGACCATCAAGGATTTTGCGGATTCGGTATTGACGATCTATCAGATG
GCGAAGTATTTCGCTGTCGAGAAAAAGCGGGCATGGCTGGCCGAATACGAGTTGGACTCC
TTCTATACTCAACCCGATACAGGGTACCTGCAGTTTTACGATAATGCATACGAGGATATA
GTCCAGGTGTACAATAAACTCAGGAACTACCTCACTAAGAAACCATACTCCGAAGAAAAA
TGGAAACTTAATTTTGAGAATAGTACACTGGCCAATGGATGGGACAAGAACAAGGAATCA
GACAACTCCGCTGTAATTCTCCAGAAGGGTGGCAAGTATTATCTGGGACTGATAACAAAG
GGCCATAACAAGATTTTCGATGACCGTTTTCAGGAGAAGTTTATAGTGGGCATAGAGGGT
GGCAAGTATGAAAAAATAGTCTACAAGTTCTTTCCCGATCAGGCGAAGATGTTCCCCAAA
GTATGCTTCAGTGCTAAAGGCCTCGAGTTTTTCCGGCCATCTGAAGAGATACTCCGCATC
TATAATAACGCAGAGTTTAAAAAGGGAGAGACGTACTCAATCGACTCGATGCAGAAACTC
ATTGACTTCTACAAAGATTGTCTCACAAAATACGAGGGCTGGGCTTGCTACACGTTTCGG
CACTTGAAGCCAACCGAGGAATATCAAAACAACATCGGGGAGTTCTTCCGTGACGTCGCC
GAAGACGGCTATAGAATTGACTTTCAGGGCATAAGTGATCAGTATATTCACGAGAAGAAT
GAGAAAGGTGAGTTGCATCTTTTCGAAATCCACAATAAAGACTGGAATCTTGACAAGGCT
CGCGATGGAAAATCAAAGACTACCCAGAAGAATCTTCATACACTTTACTTCGAGTCCCTC
TTTTCCAACGACAACGTCGTACAGAATTTCCCAATAAAACTGAACGGCCAGGCCGAAATT
TTTTACAGGCCCAAAACCGAAAAAGATAAACTGGAATCCAAGAAAGACAAGAAGGGAAAT
AAGGTGATAGATCACAAAAGGTATTCCGAGAACAAGATTTTTTTCCACGTACCTCTTACC
CTGAACAGAACGAAGAACGACTCTTATAGATTCAATGCCCAGATAAACAACTTTCTCGCA
AACAACAAAGATATCAATATTATCGGCGTCGATAGAGGTGAGAAGCACTTGGTATATTAT
TCTGTGATCACGCAAGCATCCGATATCTTGGAGTCCGGTTCTTTGAACGAACTGAATGGT
GTCAACTACGCCGAGAAACTCGGTAAGAAAGCTGAGAATCGGGAGCAGGCTAGAAGGGAC
TGGCAGGACGTTCAGGGTATCAAGGACCTGAAGAAGGGCTACATTTCTCAGGTGGTTCGA
AAACTGGCTGATTTGGCCATTAAGCACAATGCAATCATCATTTTAGAAGATTTGAACATG
CGGTTTAAACAAGTCAGGGGGGGGATAGAGAAATCAATTTACCAACAGCTGGAAAAAGCT
CTGATTGATAAACTCTCTTTTTTGGTTGATAAGGGCGAAAAGAACCCCGAGCAAGCAGGA
CATCTCCTTAAAGCCTATCAACTGAGCGCACCTTTCGAGACATTCCAGAAGATGGGAAAG
CAAACCGGCATCATTTTCTATACCCAGGCTTCCTATACATCCAAGTCTGATCCAGTGACT
GGGTGGAGACCCCATCTCTACCTCAAGTACTTTTCTGCCAAAAAAGCTAAGGACGACATT
GCTAAGTTCACAAAAATCGAGTTCGTGAACGACAGGTTCGAGCTGACTTATGACATAAAA
GATTTCCAGCAGGCCAAGGAGTACCCAAACAAGACAGTTTGGAAAGTGTGTTCCAATGTG
GAGAGGTTTCGGTGGGACAAGAATCTGAATCAGAATAAAGGGGGATATACTCACTACACC
AACATTACCGAGAACATCCAAGAGTTGTTCACCAAATACGGCATCGACATTACTAAAGAT
CTGCTGACACAGATCTCCACCATCGATGAGAAGCAGAACACATCTTTCTTCCGGGATTTC
ATCTTTTATTTTAACTTGATCTGTCAGATTAGAAATACCGACGACAGTGAGATAGCTAAA
AAAAACGGGAAAGACGATTTCATTCTCTCTCCCGTGGAGCCGTTTTTTGACTCCCGCAAA
GACAATGGCAATAAGCTTCCGGAAAACGGGGACGATAACGGCGCCTACAACATCGCTCGT
AAGGGAATCGTTATCCTCAATAAAATAAGCCAGTATTCCGAGAAGAACGAGAATTGTGAA
AAAATGAAGTGGGGGGACCTTTACGTCAGCAACATCGATTGGGATAACTTTGTGACACAA
GCCAATGCGAGACACTAG
SEQ ATGGAAAACTTCAAAAACCTCTACCCCATCAACAAGACCTTGAGGTTTGAGCTCCGGCCA
ID TATGGGAAGACACTGGAGAACTTCAAAAAGTCCGGTCTGCTGGAAAAGGATGCTTTTAAG
NO: GCTAACTCTAGGAGGTCTATGCAGGCCATTATCGATGAGAAATTCAAGGAGACCATAGAG
157 GAGCGTCTGAAATATACTGAGTTTTCCGAGTGTGACCTAGGAAATATGACCAGTAAGGAC
AAAAAGATCACCGACAAGGCAGCGACAAACCTGAAGAAACAGGTGATTTTAAGCTTTGAT
GATGAGATTTTCAATAACTACTTGAAGCCGGACAAAAACATCGACGCTCTGTTCAAGAAT
GATCCAAGCAACCCGGTCATCTCTACTTTCAAGGGCTTCACCACATACTTTGTAAATTTC
TTCGAAATACGGAAACACATCTTCAAGGGAGAGTCTTCCGGTAGCATGGCTTACAGAATA
ATCGATGAGAACCTAACTACATATCTAAACAATATCGAGAAGATCAAGAAATTGCCTGAA
GAACTGAAATCTCAGCTTGAGGGAATCGATCAAATTGACAAACTGAACAACTATAACGAG
TTCATCACCCAGTCCGGCATTACTCATTATAACGAAATTATTGGAGGGATTTCGAAGTCT
GAAAATGTCAAAATTCAAGGCATTAACGAAGGGATTAATCTTTACTGTCAAAAGAATAAA
GTGAAGCTACCACGCTTAACTCCTCTGTATAAGATGATTCTCTCTGATCGGGTCTCTAAT
TCCTTTGTGCTGGATACCATTGAAAATGATACCGAGTTAATTGAAATGATCTCTGATCTG
ATAAATAAGACAGAGATAAGTCAGGATGTTATTATGTCCGACATCCAAAATATTTTCATC
AAATATAAACAACTCGGCAACTTGCCGGGGATTAGCTACTCATCTATAGTGAATGCTATC
TGTTCGGATTACGACAATAACTTTGGTGACGGCAAACGTAAAAAAAGCTATGAGAATGAT
CGCAAAAAACACCTCGAGACTAACGTGTATAGCATTAACTATATCTCAGAGTTACTGACA
GACACCGACGTCTCCAGCAACATAAAGATGCGGTACAAAGAGCTGGAGCAGAATTATCAG
GTATGCAAGGAAAATTTCAACGCCACTAACTGGATGAACATCAAAAACATTAAGCAGTCT
GAGAAAACCAATCTGATCAAGGACCTTCTTGACATCCTCAAGAGCATCCAGCGGTTTTAT
GATTTGTTTGACATCGTGGATGAAGACAAAAATCCTAGTGCTGAGTTCTATACCTGGCTG
TCTAAAAACGCGGAGAAACTGGACTTCGAGTTTAATTCAGTGTACAACAAGAGCAGGAAC
TACCTCACGAGAAAGCAGTACTCCGATAAAAAGATTAAGTTGAACTTCGATAGTCCTACT
CTCGCCAAGGGGTGGGATGCGAACAAAGAAATTGATAATAGCACAATTATCATGAGGAAG
TTCAACAACGACCGGGGCGATTACGATTACTTCTTGGGGATCTGGAATAAGAGCACACCT
GCCAACGAAAAGATCATCCCATTAGAGGATAATGGACTGTTTGAAAAAATGCAATATAAG
CTGTATCCCGATCCTAGTAAAATGCTGCCAAAGCAATTCCTTTCTAAGATCTGGAAAGCT
AAACATCCAACTACACCCGAGTTTGATAAGAAGTACAAAGAAGGTCGGCACAAGAAGGGG
CCTGATTTTGAGAAAGAGTTTCTGCACGAGTTGATCGATTGCTTTAAGCATGGATTGGTA
AACCACGACGAAAAATATCAGGATGTGTTCGGGTTCAATCTGCGCAACACGGAAGACTAC
AACTCTTATACAGAGTTTCTGGAGGACGTCGAAAGGTGCAACTATAATCTTAGTTTCAAT
AAAATCGCTGACACGTCTAACTTGATAAATGATGGGAAACTCTATGTTTTTCAGATCTGG
AGCAAGGATTTCAGCATAGATAGCAAGGGAACAAAAAACTTGAACACAATATACTTTGAA
TCCCTCTTCTCGGAGGAAAATATGATCGAGAAGATGTTCAAGCTCTCAGGGGAAGCCGAA
ATATTCTATCGTCCAGCAAGTTTGAATTATTGTGAAGATATTATCAAGAAGGGACACCAC
CACGCCGAACTGAAGGACAAATTCGACTATCCCATCATCAAGGACAAGCGATATAGCCAG
GACAAATTTTTTTTTCATGTCCCCATGGTTATCAACTACAAAAGCGAGAAGTTAAACTCC
AAATCACTTAACAATAGGACGAACGAAAATTTAGGCCAATTCACGCACATCATCGGTATC
GACCGCGGAGAGCGACATCTCATCTACCTGACCGTGGTGGATGTGTCCACCGGTGAGATC
GTTGAGCAAAAGCACCTGGATGAAATTATAAATACAGATACAAAAGGCGTCGAGCATAAA
ACTCATTATCTCAATAAATTAGAAGAGAAGTCCAAGACGCGGGATAATGAAAGAAAGTCC
TGGGAAGCAATCGAGACGATTAAGGAGCTGAAAGAAGGCTATATTAGCCACGTGATCAAT
GAAATCCAGAAATTGCAGGAAAAGTATAACGCACTGATAGTGATGGAGAACCTCAATTAT
GGGTTTAAGAACTCGCGTATCAAAGTGGAAAAGCAGGTCTACCAGAAATTCGAGACCGCC
CTGATTAAAAAGTTTAATTACATCATTGACAAGAAAGATCCTGAAACCTACATTCATGGA
TACCAACTGACGAATCCAATCACTACACTCGATAAAATTGGTAACCAGAGCGGTATTGTG
TTGTACATTCCGGCTTGGAATACAAGCAAGATTGATCCAGTCACTGGTTTCGTTAACCTC
CTGTATGCAGACGATTTGAAATACAAGAACCAGGAGCAGGCTAAAAGCTTTATCCAGAAA
ATCGATAATATCTACTTCGAAAATGGTGAGTTTAAATTTGATATAGATTTCAGCAAATGG
AACAACCGCTACTCAATTAGCAAGACGAAATGGACACTGACAAGCTACGGAACCCGGATA
CAGACGTTCCGAAACCCCCAGAAAAATAACAAGTGGGACAGCGCCGAGTATGACCTGACC
GAAGAGTTTAAATTAATCCTGAACATCGATGGTACTCTGAAATCTCAGGATGTGGAAACC
TATAAGAAATTCATGTCTTTATTCAAGCTGATGTTGCAGCTGCGAAACTCCGTTACTGGA
ACAGACATTGACTACATGATTAGCCCTGTGACAGATAAAACTGGAACCCACTTTGATTCA
CGGGAGAATATCAAGAACCTGCCCGCCGATGCTGATGCGAACGGAGCTTACAACATTGCT
AGGAAGGGCATCATGGCAATCGAGAATATTATGAACGGCATTAGCGACCCTCTGAAGATC
AGTAATGAGGACTACCTGAAGTACATTCAGAACCAACAAGAGTAA
SEQ ATGACCCAGTTTGAGGGTTTCACCAATCTTTATCAGGTGTCAAAAACACTCAGATTTGAG
ID CTCATCCCACAGGGTAAAACTTTAAAGCATATTCAAGAGCAGGGCTTTATAGAGGAAGAC
NO: AAAGCCAGAAACGACCATTATAAGGAACTAAAACCGATCATTGACCGCATCTACAAAACC
158 TATGCCGACCAATGCCTTCAGCTCGTCCAACTCGATTGGGAGAATCTGAGCGCCGCTATT
GACAGCTACAGGAAGGAGAAGACCGAGGAGACTAGAAACGCCCTGATCGAGGAGCAGGCG
ACCTATAGAAACGCTATTCACGATTATTTTATCGGCCGCACCGACAATTTGACAGATGCC
ATCAACAAGCGGCACGCCGAAATTTATAAGGGGTTATTTAAGGCCGAGCTGTTCAATGGA
AAAGTACTGAAACAGCTGGGCACCGTAACAACCACCGAACACGAGAATGCTCTGTTGAGG
TCCTTCGACAAGTTTACTACCTACTTTAGCGGCTTCTACGAAAACCGTAAAAACGTGTTT
TCCGCGGAGGATATTTCAACAGCCATTCCTCATAGGATCGTGCAGGATAATTTCCCCAAG
TTTAAGGAGAACTGCCATATCTTTACCAGACTTATCACTGCTGTGCCAAGTTTACGAGAA
CACTTCGAGAATGTTAAGAAGGCTATAGGCATATTCGTTTCCACCTCCATCGAAGAAGTA
TTCAGTTTTCCATTCTACAATCAGTTACTCACGCAGACCCAGATAGATCTCTACAATCAG
CTGCTCGGAGGCATTTCTAGAGAAGCAGGCACGGAAAAGATCAAGGGCTTAAATGAAGTA
CTCAATCTTGCAATTCAGAAGAACGATGAGACAGCACACATTATTGCATCTCTCCCTCAC
AGATTCATTCCCCTGTTCAAACAGATCCTGTCCGATCGCAACACACTAAGCTTTATACTT
GAGGAGTTTAAGTCAGATGAGGAAGTGATCCAGAGCTTCTGTAAGTATAAGACTTTGCTC
CGTAATGAAAACGTGCTTGAGACAGCAGAGGCTCTCTTTAACGAGTTGAATTCCATCGAC
CTGACACACATTTTTATCAGCCATAAAAAGCTGGAAACGATTAGCTCTGCCTTGTGCGAC
CACTGGGACACCCTGCGTAACGCCCTCTATGAAAGGCGCATTTCCGAGCTCACCGGGAAG
ATCACAAAAAGTGCCAAGGAAAAAGTCCAGAGGTCCCTTAAACATGAAGACATCAACCTA
CAAGAGATCATCTCTGCGGCTGGGAAAGAGCTGTCAGAAGCATTTAAACAGAAGACTTCC
GAGATCCTGAGCCACGCACACGCCGCATTAGACCAGCCCCTGCCTACAACTCTTAAAAAA
CAGGAGGAGAAGGAGATTTTAAAGAGCCAGCTGGACTCATTACTCGGCCTGTATCATCTC
CTGGACTGGTTCGCCGTGGACGAATCCAACGAGGTGGACCCAGAATTTAGCGCCAGGCTG
ACAGGAATTAAACTGGAAATGGAGCCAAGTTTGAGCTTTTACAACAAGGCTCGGAACTAT
GCCACTAAAAAGCCCTACAGCGTGGAAAAGTTCAAGCTGAATTTTCAGATGCCGACCCTG
GCTTCCGGGTGGGATGTTAATAAGGAAAAGAATAATGGGGCTATACTGTTCGTCAAAAAT
GGTCTCTACTACCTGGGAATCATGCCCAAACAGAAGGGCAGGTACAAAGCCCTTTCGTTT
GAGCCGACCGAAAAAACCAGCGAAGGCTTTGATAAGATGTATTACGACTATTTCCCAGAT
GCAGCCAAGATGATCCCAAAATGTAGCACTCAGTTGAAGGCGGTAACCGCTCACTTTCAG
ACACACACCACTCCTATCTTGCTCTCCAACAACTTTATTGAGCCGCTGGAGATCACGAAG
GAAATCTACGACCTTAACAACCCAGAGAAGGAACCCAAGAAATTCCAAACAGCTTATGCT
AAGAAGACTGGGGATCAAAAGGGCTATCGAGAGGCTTTGTGTAAGTGGATTGACTTTACA
CGGGATTTCCTGAGTAAGTATACCAAGACCACATCTATTGACCTGTCCTCACTGAGACCT
TCCTCACAATATAAGGATCTCGGAGAGTATTATGCCGAACTCAACCCTCTACTCTATCAC
ATCTCTTTCCAGAGGATCGCCGAAAAGGAAATTATGGACGCCGTCGAGACAGGCAAGCTG
TACCTCTTCCAGATTTACAACAAGGATTTCGCAAAGGGCCACCACGGAAAACCCAATTTG
CACACTTTGTACTGGACAGGGCTCTTCTCTCCCGAAAATTTGGCCAAAACTTCAATAAAA
CTGAACGGGCAAGCCGAGCTGTTCTATCGGCCCAAGTCACGTATGAAGCGGATGGCCCAC
CGGCTGGGCGAGAAGATGCTCAACAAGAAACTGAAGGATCAGAAGACGCCCATACCAGAC
ACTCTTTACCAAGAGCTGTATGACTACGTGAATCACAGACTGAGTCACGACCTGTCTGAT
GAAGCCCGGGCTCTTCTTCCAAATGTGATTACCAAAGAAGTTTCCCACGAAATTATCAAG
GACCGGCGCTTCACCTCTGACAAATTCTTTTTCCACGTCCCAATCACCCTCAACTACCAG
GCAGCCAATTCCCCTTCAAAGTTTAACCAGCGTGTGAATGCCTACCTGAAAGAGCATCCG
GAGACCCCCATCATAGGGATAGACAGAGGAGAGCGGAATCTTATCTACATTACTGTGATT
GACAGCACAGGTAAGATCTTGGAGCAGAGATCTTTAAATACAATCCAGCAGTTTGACTAC
CAGAAGAAACTGGATAACCGAGAGAAGGAAAGGGTTGCTGCAAGACAGGCCTGGTCAGTG
GTCGGCACCATCAAAGACCTGAAGCAGGGCTACTTATCCCAAGTAATTCACGAAATTGTC
GATCTTATGATTCATTATCAAGCCGTTGTTGTGCTGGAGAACCTGAATTTTGGCTTCAAA
AGCAAACGAACAGGTATCGCCGAGAAAGCCGTGTATCAGCAGTTCGAAAAGATGCTCATA
GACAAGCTGAACTGCTTAGTGCTGAAGGATTATCCTGCTGAGAAGGTCGGCGGCGTACTT
AACCCATACCAGCTGACCGATCAGTTCACTAGTTTCGCCAAGATGGGAACGCAAAGTGGC
TTCCTTTTCTACGTGCCCGCTCCCTACACGAGTAAGATCGACCCTCTGACCGGCTTCGTC
GACCCATTCGTCTGGAAGACCATCAAGAATCACGAATCACGGAAACACTTCTTAGAGGGG
TTTGACTTCCTGCACTACGACGTGAAGACAGGGGACTTCATCTTACACTTTAAGATGAAT
CGAAACCTCTCCTTCCAGCGGGGCCTGCCTGGTTTCATGCCCGCATGGGACATCGTGTTT
GAGAAAAACGAGACACAGTTTGACGCTAAGGGAACCCCCTTTATTGCGGGGAAGCGGATT
GTCCCAGTCATCGAAAACCATCGGTTCACCGGGCGATACCGGGATCTGTACCCGGCCAAC
GAGCTCATCGCGCTGCTGGAGGAGAAGGGTATTGTGTTTAGGGATGGATCCAACATTCTG
CCTAAGTTGCTGGAAAATGATGATTCGCACGCCATTGATACCATGGTTGCACTGATTAGA
TCCGTACTGCAGATGAGGAATAGCAATGCTGCAACCGGGGAGGATTATATTAATTCCCCA
GTGCGAGATCTGAATGGTGTCTGTTTTGACTCGCGCTTTCAGAATCCAGAATGGCCAATG
GATGCAGACGCTAACGGGGCGTACCACATTGCTCTGAAAGGCCAGCTACTCCTGAACCAC
CTCAAGGAGAGCAAAGATCTGAAGCTGCAGAACGGCATTTCCAACCAAGACTGGCTCGCC
TACATACAAGAACTGCGCAATTAA
SEQ ATGGCTGTCAAATCCATCAAGGTTAAATTACGGCTTGATGACATGCCCGAGATCCGCGCC
ID GGGCTCTGGAAACTCCATAAAGAAGTGAATGCTGGCGTTAGATACTACACAGAATGGCTC
NO: TCCCTGCTGCGCCAGGAAAATTTGTACCGCCGGTCACCTAATGGAGATGGAGAGCAGGAA
159 TGCGATAAAACAGCAGAAGAGTGCAAAGCCGAATTGCTGGAGCGACTGCGGGCACGGCAG
GTTGAGAATGGACACCGAGGTCCGGCGGGATCGGACGACGAGCTGCTCCAGCTCGCCAGA
CAATTATATGAACTGCTGGTGCCTCAGGCTATTGGGGCAAAGGGTGACGCACAGCAGATT
GCTAGAAAATTTCTGTCTCCCCTCGCCGACAAAGACGCTGTCGGCGGCCTTGGGATAGCC
AAAGCCGGCAACAAACCCCGATGGGTGCGCATGAGGGAGGCTGGTGAGCCTGGCTGGGAG
GAAGAAAAGGAAAAGGCCGAAACCAGAAAGTCCGCCGACAGGACCGCGGACGTACTCCGA
GCATTGGCCGATTTTGGGCTGAAGCCCTTAATGCGAGTCTACACCGATAGTGAAATGTCT
AGCGTGGAGTGGAAGCCATTACGCAAAGGGCAGGCAGTGCGGACGTGGGACCGTGACATG
TTCCAGCAAGCCATCGAGCGAATGATGAGCTGGGAGAGCTGGAACCAGAGAGTGGGGCAG
GAGTATGCCAAGCTGGTCGAGCAGAAAAACCGGTTTGAGCAAAAAAATTTTGTAGGTCAG
GAACACCTGGTGCATCTCGTTAACCAGCTCCAGCAAGATATGAAGGAAGCTTCGCCTGGA
TTAGAGAGCAAAGAGCAGACTGCACACTATGTAACCGGAAGAGCACTGAGGGGCAGTGAC
AAAGTGTTCGAAAAATGGGGAAAACTGGCTCCCGATGCCCCCTTTGACCTGTACGACGCA
GAAATAAAAAACGTGCAGCGGCGAAACACCAGGCGATTTGGTAGCCATGATCTGTTCGCC
AAATTGGCAGAGCCGGAATATCAGGCTCTTTGGCGAGAAGACGCATCATTTCTCACTAGG
TACGCGGTCTATAACTCCATTTTGAGGAAATTGAACCACGCAAAAATGTTTGCCACCTTC
ACGTTGCCTGACGCCACCGCTCATCCCATTTGGACACGGTTTGATAAGCTGGGCGGCAAT
CTGCATCAGTATACATTCCTGTTTAACGAGTTTGGAGAGCGAAGACATGCGATACGATTC
CACAAGCTACTGAAGGTCGAAAATGGCGTGGCACGTGAGGTGGACGATGTCACCGTGCCC
ATCAGCATGAGCGAACAGCTGGATAATTTGTTGCCGCGGGACCCAAATGAACCTATAGCC
CTTTATTTTAGGGACTACGGGGCGGAGCAACATTTCACTGGGGAGTTTGGCGGCGCAAAA
ATTCAGTGCCGACGCGACCAGCTCGCCCACATGCATAGAAGACGCGGGGCCCGGGACGTA
TACCTTAACGTCTCTGTGAGGGTGCAGTCCCAGTCAGAGGCAAGAGGGGAACGCAGACCA
CCTTACGCAGCAGTATTCAGGCTGGTAGGCGATAACCACCGGGCGTTTGTACACTTTGAT
AAACTTTCTGACTACCTGGCCGAACACCCGGATGACGGCAAATTAGGATCGGAGGGGCTG
CTTAGCGGCCTGCGTGTGATGAGCGTCGATCTGGGGCTACGGACCTCTGCTTCCATCTCT
GTGTTCCGTGTGGCCCGAAAGGACGAGTTGAAACCTAATTCGAAGGGCCGTGTACCATTC
TTTTTCCCTATTAAGGGAAATGATAATCTCGTCGCGGTGCACGAGCGTTCCCAACTGCTG
AAACTGCCTGGCGAGACCGAGTCCAAAGATCTCAGAGCAATCCGGGAGGAGCGACAACGT
ACACTTAGGCAACTCCGCACCCAGCTGGCCTATCTGCGCTTGCTGGTGCGGTGCGGCTCC
GAGGATGTAGGGAGAAGAGAGCGAAGCTGGGCAAAGCTGATAGAGCAACCAGTTGACGCC
GCGAATCACATGACCCCCGACTGGCGCGAAGCGTTTGAAAATGAGCTGCAGAAGTTGAAA
TCTCTGCATGGGATTTGCTCAGATAAGGAGTGGATGGACGCCGTATACGAGTCTGTTCGC
CGGGTATGGCGGCACATGGGGAAGCAGGTGAGAGATTGGAGAAAGGACGTTCGCTCTGGG
GAACGGCCGAAAATTCGGGGATACGCAAAGGATGTCGTGGGCGGCAATAGCATTGAGCAG
ATCGAGTACCTGGAAAGGCAATACAAATTTCTGAAATCTTGGTCTTTCTTTGGGAAGGTA
AGCGGACAAGTTATCAGAGCCGAAAAGGGATCTCGCTTTGCTATCACATTGAGGGAACAC
ATTGATCACGCCAAAGAAGACAGGTTGAAAAAGTTGGCTGATCGCATTATCATGGAAGCA
CTCGGTTACGTCTACGCCCTTGATGAGCGCGGTAAAGGGAAGTGGGTAGCCAAGTATCCC
CCATGTCAGCTGATCCTGCTCGAGGAACTTTCTGAGTATCAGTTCAATAACGACCGTCCT
CCCTCCGAAAATAATCAGCTCATGCAATGGTCCCACCGGGGTGTGTTCCAAGAACTGATC
AATCAGGCTCAGGTGCACGACCTCCTCGTAGGCACTATGTATGCAGCCTTTAGCTCCCGT
TTTGACGCGCGCACAGGCGCCCCTGGAATACGATGTAGGCGAGTTCCCGCACGGTGCACT
CAAGAACATAACCCGGAGCCTTTCCCATGGTGGCTCAATAAGTTTGTTGTGGAGCATACC
CTCGACGCTTGCCCATTGAGGGCGGATGACTTGATTCCCACAGGCGAGGGGGAGATCTTC
GTGAGCCCATTTTCTGCCGAAGAAGGGGATTTCCACCAAATACATGCCGACTTGAATGCT
GCCCAAAATCTGCAGCAAAGGCTGTGGTCAGACTTCGACATCTCGCAAATCAGACTGCGG
TGTGACTGGGGCGAAGTAGACGGCGAGCTGGTGCTGATACCTAGACTGACGGGTAAGCGT
ACCGCCGATAGCTATAGTAATAAGGTTTTTTATACGAATACGGGGGTGACATATTACGAG
CGTGAGAGAGGCAAGAAGCGTCGGAAGGTGTTCGCGCAGGAGAAGCTGAGCGAAGAGGAG
GCGGAGCTACTGGTAGAGGCAGATGAGGCAAGAGAAAAGTCCGTCGTCCTGATGCGGGAT
CCTAGCGGGATTATTAACAGAGGTAATTGGACACGGCAGAAAGAATTCTGGAGCATGGTG
AATCAAAGAATCGAGGGTTACCTGGTGAAGCAAATTCGAAGCCGGGTGCCCCTTCAAGAC
AGCGCATGTGAAAACACTGGGGACATCTAG
SEQ ATGGCTACTCGGTCCTTCATCCTGAAAATCGAGCCAAATGAAGAGGTGAAAAAGGGCCTG
ID TGGAAGACCCATGAGGTACTTAACCACGGCATAGCATACTATATGAATATCCTAAAACTT
NO: ATACGGCAGGAGGCTATCTACGAGCATCACGAGCAAGATCCTAAAAATCCAAAGAAGGTT
160 AGTAAGGCTGAAATCCAGGCTGAATTGTGGGACTTCGTGCTGAAGATGCAGAAATGCAAC
AGTTTCACGCATGAAGTTGATAAGGACGTCGTGTTTAATATACTCCGGGAGCTGTACGAA
GAACTGGTACCAAGCTCTGTGGAAAAGAAAGGAGAGGCCAACCAGCTAAGTAATAAGTTC
CTCTATCCTCTCGTGGACCCCAATTCACAGAGCGGCAAAGGTACCGCATCTTCTGGGAGG
AAACCACGCTGGTACAACTTGAAGATCGCTGGCGATCCCAGCTGGGAGGAGGAAAAGAAG
AAATGGGAAGAGGATAAAAAGAAAGACCCCCTGGCCAAAATCTTAGGCAAGCTCGCCGAG
TACGGTCTGATTCCACTTTTCATCCCGTTCACAGATAGCAATGAGCCGATCGTCAAGGAG
ATTAAGTGGATGGAAAAGAGCCGCAATCAGAGTGTGCGGAGGCTGGACAAAGACATGTTT
ATTCAGGCCCTGGAACGCTTCCTTAGCTGGGAAAGCTGGAACCTGAAGGTTAAGGAAGAG
TACGAAAAAGTCGAGAAGGAGCATAAGACTTTGGAGGAGCGCATCAAAGAAGACATCCAG
GCCTTTAAGTCTCTAGAACAGTATGAGAAAGAACGGCAGGAACAGCTGCTGCGTGATACA
CTGAACACAAACGAATATCGCCTGAGCAAGAGGGGACTCAGAGGCTGGAGAGAAATCATT
CAAAAGTGGCTCAAAATGGATGAAAATGAGCCGTCTGAAAAATACCTTGAAGTTTTCAAG
GACTACCAGCGGAAGCACCCTAGAGAAGCCGGCGACTATAGTGTTTACGAATTCTTGAGC
AAGAAGGAGAATCATTTTATATGGAGGAATCACCCGGAGTACCCATATCTGTACGCAACC
TTCTGCGAAATCGACAAGAAAAAAAAAGACGCCAAGCAACAGGCTACATTTACTCTGGCC
GACCCTATCAATCACCCTCTATGGGTCCGGTTTGAGGAGCGCTCCGGAAGCAATCTGAAT
AAATATCGTATTCTGACTGAACAGTTACACACAGAGAAGCTCAAGAAGAAACTTACGGTG
CAGCTGGACCGCCTGATATACCCAACAGAGTCCGGAGGATGGGAAGAGAAAGGAAAGGTT
GACATCGTACTGCTTCCATCTCGTCAGTTTTACAACCAGATATTCCTGGACATCGAGGAG
AAGGGGAAACACGCCTTCACATACAAGGACGAGTCCATAAAGTTCCCACTGAAGGGTACT
TTAGGCGGTGCTAGGGTGCAGTTCGACCGCGATCACCTGAGACGGTACCCCCACAAGGTG
GAGAGCGGGAACGTGGGACGAATCTACTTTAATATGACAGTGAACATTGAACCCACAGAG
AGTCCAGTTAGTAAATCCCTGAAAATTCACCGTGACGACTTTCCGAAATTTGTGAATTTC
AAGCCAAAGGAGCTTACGGAGTGGATCAAGGATTCAAAGGGAAAGAAGCTGAAATCTGGT
ATCGAATCTCTCGAGATCGGTCTCCGTGTCATGAGCATCGATCTGGGACAGCGCCAGGCA
GCTGCCGCCAGTATATTCGAGGTGGTAGACCAAAAGCCTGACATCGAGGGAAAGCTCTTC
TTCCCAATCAAAGGCACAGAGCTGTATGCGGTGCACCGGGCGTCCTTTAATATAAAGCTG
CCCGGTGAAACCCTGGTGAAGTCACGGGAGGTGCTTAGAAAAGCGCGAGAGGATAACCTC
AAACTGATGAACCAAAAACTGAACTTTCTGAGGAACGTCCTGCACTTTCAGCAGTTCGAA
GATATTACCGAACGCGAAAAGAGAGTAACCAAGTGGATATCTCGTCAAGAGAACAGCGAC
GTCCCGTTAGTCTATCAGGACGAACTCATCCAAATACGGGAGTTGATGTATAAGCCCTAC
AAGGATTGGGTCGCCTTTCTTAAGCAGCTTCACAAACGCCTAGAGGTCGAAATAGGTAAA
GAGGTGAAACATTGGCGGAAGTCGCTCAGCGACGGGAGGAAGGGACTTTATGGCATCTCT
TTGAAGAACATTGACGAAATCGATAGAACCAGAAAATTTTTGTTGAGATGGTCCCTCCGA
CCCACCGAGCCTGGAGAGGTGAGGCGGTTAGAACCAGGACAGAGGTTCGCTATCGATCAG
CTGAATCACCTCAATGCTCTGAAGGAGGACCGCCTCAAGAAAATGGCCAATACAATCATA
ATGCACGCCCTTGGCTACTGCTACGACGTCCGAAAGAAGAAGTGGCAGGCCAAGAATCCC
GCCTGTCAAATTATCCTTTTTGAGGATCTTAGCAATTACAACCCCTATGAAGAGCGGTCC
AGATTCGAAAATAGTAAGCTCATGAAGTGGAGCCGCAGGGAGATCCCGCGCCAAGTGGCC
CTTCAGGGGGAAATTTATGGGCTGCAGGTAGGCGAGGTCGGGGCCCAATTCTCCTCGCGC
TTTCATGCGAAAACTGGAAGTCCTGGAATCCGGTGCTCAGTGGTGACAAAGGAGAAGTTG
CAAGACAATCGGTTTTTTAAAAACTTACAGCGGGAGGGAAGGCTGACCCTGGATAAGATA
GCCGTACTTAAGGAAGGAGATCTGTACCCTGACAAAGGCGGTGAAAAGTTCATTAGCTTG
AGCAAGGACCGAAAACTTGTGACCACCCACGCTGACATCAATGCGGCACAGAACCTGCAG
AAGAGATTTTGGACTCGCACCCACGGATTCTACAAAGTTTACTGCAAAGCATATCAAGTA
GACGGACAGACCGTATACATCCCCGAGTCCAAAGATCAGAAGCAGAAAATTATTGAAGAG
TTTGGGGAAGGGTACTTTATCCTGAAGGATGGTGTCTACGAATGGGGCAACGCTGGTAAA
CTTAAAATTAAGAAGGGCAGCTCTAAACAGTCCTCCAGCGAGTTAGTTGATTCTGATATT
CTGAAAGACAGTTTCGACCTGGCCAGCGAACTTAAAGGGGAAAAATTAATGCTGTACCGG
GACCCCAGCGGAAACGTCTTTCCATCCGATAAGTGGATGGCCGCTGGAGTGTTCTTTGGC
AAGTTAGAGAGGATTCTCATAAGTAAGCTGACCAACCAATACTCAATCTCCACAATCGAG
GATGACTCATCCAAGCAGTCTATGTGA
SEQ ATGCCTACACGCACTATCAACCTGAAACTGGTTCTTGGCAAGAATCCAGAGAATGCTACC
ID CTTCGTCGGGCACTATTTTCAACGCATAGACTGGTGAATCAGGCTACCAAACGGATTGAA
NO: GAGTTCCTCTTGCTTTGTCGGGGGGAAGCATATAGGACGGTGGATAATGAGGGGAAAGAG
161 GCTGAAATTCCGAGACACGCCGTGCAGGAGGAAGCTCTTGCGTTTGCAAAGGCCGCTCAA
CGGCACAATGGTTGCATCTCTACTTATGAAGACCAGGAAATCCTGGATGTGCTCCGGCAA
CTGTATGAAAGGCTGGTGCCTTCTGTGAATGAAAATAATGAAGCAGGGGACGCTCAAGCC
GCAAACGCGTGGGTGTCGCCACTGATGTCCGCCGAGTCCGAGGGAGGGCTCAGCGTTTAC
GACAAGGTGCTGGACCCACCCCCAGTGTGGATGAAACTCAAAGAGGAAAAAGCTCCGGGC
TGGGAGGCTGCTTCCCAGATCTGGATCCAGTCCGACGAAGGGCAGTCCCTTCTTAACAAG
CCTGGTTCGCCCCCGCGGTGGATTAGGAAACTGAGGTCAGGCCAGCCTTGGCAGGACGAT
TTTGTTAGCGACCAGAAAAAGAAGCAGGACGAGCTGACAAAGGGGAATGCGCCACTGATC
AAACAATTAAAGGAAATGGGCTTATTGCCTCTTGTGAATCCCTTTTTTAGACATCTGCTT
GACCCGGAGGGGAAGGGGGTGTCACCTTGGGACAGACTCGCTGTTAGGGCCGCTGTCGCT
CATTTCATATCATGGGAATCATGGAACCACCGGACACGCGCCGAATACAATAGTTTGAAG
CTGCGGAGGGATGAGTTCGAAGCAGCTTCCGACGAATTCAAGGACGACTTCACGCTGCTT
CGGCAGTACGAGGCTAAGAGGCACTCCACACTGAAGAGTATAGCTTTAGCCGATGATTCA
AACCCTTATAGGATCGGCGTACGCTCCCTCCGCGCTTGGAACCGCGTCCGCGAGGAGTGG
ATCGACAAGGGAGCGACCGAGGAGCAGCGGGTCACCATTCTCAGCAAGTTGCAGACCCAA
CTAAGGGGCAAATTTGGAGATCCTGACTTGTTCAACTGGCTGGCGCAGGACCGGCACGTG
CACCTCTGGAGCCCTAGAGATAGTGTTACCCCACTGGTTAGGATCAACGCTGTTGACAAA
GTATTGCGACGGAGAAAACCGTACGCCTTGATGACTTTTGCCCACCCAAGATTCCACCCT
CGGTGGATACTTTACGAAGCCCCAGGGGGCAGCAATCTCCGCCAGTATGCACTGGATTGT
ACCGAAAATGCTCTGCACATTACACTGCCTCTGCTGGTTGACGATGCACATGGCACATGG
ATTGAGAAAAAAATTAGGGTTCCTCTTGCCCCCAGCGGCCAGATTCAGGACCTGACACTA
GAAAAGCTCGAGAAGAAGAAAAATCGTCTCTACTACCGTTCTGGGTTCCAGCAGTTTGCC
GGCCTGGCCGGAGGTGCCGAGGTGCTTTTCCATCGACCATACATGGAGCACGATGAGAGG
AGCGAGGAGAGCTTATTAGAACGCCCTGGTGCTGTTTGGTTCAAACTCACCTTGGACGTG
GCAACCCAGGCCCCTCCAAACTGGTTGGACGGAAAGGGCCGCGTCCGAACGCCCCCCGAG
GTTCACCACTTCAAGACAGCCCTCAGTAACAAGTCTAAGCACACACGGACCCTCCAGCCC
GGACTCAGAGTGTTATCCGTGGATCTGGGAATGCGCACCTTCGCCTCTTGCTCCGTATTT
GAGCTGATCGAGGGCAAACCAGAGACTGGCAGAGCGTTCCCTGTGGCCGACGAACGTTCC
ATGGATTCACCAAACAAGCTGTGGGCCAAGCACGAAAGATCCTTTAAACTCACGCTCCCC
GGCGAAACCCCCAGTCGGAAAGAAGAGGAGGAACGGAGCATTGCAAGAGCCGAAATCTAT
GCGTTGAAAAGAGATATTCAGAGATTAAAAAGTCTTCTGCGCCTGGGGGAAGAGGATAAC
GATAATAGACGCGATGCACTTCTTGAGCAATTTTTCAAGGGCTGGGGCGAGGAAGACGTG
GTTCCAGGTCAGGCCTTTCCCCGGAGTCTGTTCCAGGGGCTGGGGGCCGCCCCATTCAGA
TCCACCCCTGAGTTGTGGAGACAACACTGTCAAACCTATTATGATAAAGCAGAGGCGTGC
CTGGCTAAACACATCAGCGATTGGCGCAAGAGAACCAGGCCTAGGCCTACCTCACGTGAG
ATGTGGTACAAGACACGCTCTTATCACGGCGGAAAGTCAATCTGGATGCTGGAATACCTC
GACGCTGTGAGGAAACTGCTCTTATCCTGGAGCCTCAGAGGCCGGACCTACGGGGCTATC
AACAGACAGGACACAGCAAGGTTCGGGAGCTTAGCCAGCCGGCTCCTTCACCACATTAAC
TCACTCAAAGAGGATCGAATAAAGACCGGAGCCGACTCGATCGTGCAGGCAGCCCGAGGG
TACATCCCCCTGCCTCATGGGAAGGGCTGGGAGCAGCGATATGAACCCTGCCAGCTGATC
TTGTTTGAGGACCTTGCCCGTTATAGATTTCGCGTTGATAGACCTCGCCGTGAGAATTCT
CAGCTGATGCAGTGGAACCACAGAGCGATCGTGGCTGAGACCACTATGCAGGCCGAGCTG
TATGGACAGATCGTGGAGAACACCGCCGCAGGGTTCAGTTCTCGGTTTCATGCTGCCACC
GGAGCTCCCGGCGTCCGGTGCCGCTTCCTCTTAGAGCGTGATTTTGACAATGACCTCCCA
AAGCCCTATCTGCTGAGGGAACTGAGCTGGATGCTGGGGAACACAAAAGTAGAATCGGAG
GAGGAGAAGCTACGGCTCCTCTCCGAAAAGATACGTCCAGGCTCTCTGGTACCATGGGAC
GGAGGAGAGCAGTTCGCGACACTGCATCCTAAGAGACAGACGTTATGTGTGATTCACGCC
GATATGAACGCCGCTCAGAATCTGCAGCGAAGATTCTTTGGCCGCTGCGGCGAAGCCTTC
AGGCTGGTATGTCAGCCCCACGGGGATGATGTGCTGCGGCTGGCCTCAACCCCTGGGGCT
AGACTCTTGGGGGCACTCCAGCAGCTGGAAAATGGCCAAGGGGCTTTCGAACTCGTTCGG
GACATGGGCAGCACAAGCCAGATGAACAGATTCGTCATGAAGAGCCTGGGAAAGAAAAAG
ATCAAACCCTTACAGGACAATAATGGCGACGACGAACTGGAGGACGTGTTGTCCGTGCTG
CCAGAGGAAGACGACACAGGCCGCATCACTGTCTTCCGCGACTCAAGTGGGATATTCTTT
CCTTGCAACGTGTGGATTCCGGCCAAACAGTTCTGGCCTGCCGTCAGAGCCATGATTTGG
AAAGTGATGGCTAGTCATTCATTGGGATGA
SEQ ATGACAAAGCTGAGGCACAGACAAAAGAAGCTTACACACGACTGGGCAGGGAGCAAGAAA
ID CGTGAGGTCCTTGGGTCAAATGGAAAACTGCAGAACCCCTTGCTCATGCCTGTAAAGAAG
NO: GGGCAGGTAACAGAATTTAGAAAAGCATTCTCCGCGTACGCTCGGGCAACTAAGGGGGAA
162 ATGACCGATGGACGGAAGAACATGTTCACCCATTCTTTCGAGCCATTCAAAACAAAGCCG
TCATTGCACCAATGCGAGCTGGCCGATAAGGCTTACCAGTCTTTGCATAGTTACCTCCCC
GGTTCCCTGGCCCATTTCTTGCTTTCCGCACACGCACTGGGCTTTCGTATTTTCTCTAAA
TCTGGGGAGGCAACTGCCTTCCAGGCCAGCTCAAAAATCGAGGCCTATGAGTCCAAGCTC
GCTTCGGAGCTAGCCTGTGTCGATTTGAGTATCCAGAATTTGACGATTAGTACTCTTTTC
AACGCTCTCACAACTTCAGTTCGGGGCAAGGGGGAGGAAACTTCAGCAGATCCCCTTATC
GCACGGTTCTACACTCTCCTGACGGGCAAGCCCCTGAGCCGAGACACACAGGGCCCAGAA
CGGGACTTGGCAGAGGTCATCTCCAGAAAGATCGCCTCGTCCTTCGGCACATGGAAGGAA
ATGACTGCCAACCCTCTGCAGAGCCTCCAGTTCTTCGAAGAAGAGCTTCATGCACTAGAT
GCCAACGTGTCTTTATCTCCAGCTTTTGATGTGTTAATCAAGATGAATGATCTCCAAGGT
GATCTGAAGAACCGTACTATAGTGTTCGACCCAGATGCACCCGTGTTCGAGTACAACGCT
GAGGATCCAGCCGATATCATCATAAAGCTGACAGCTCGGTATGCGAAGGAGGCCGTCATC
AAGAATCAGAACGTGGGCAATTATGTGAAAAACGCCATTACCACCACTAATGCCAATGGG
CTGGGGTGGCTCCTCAATAAAGGGCTTTCACTACTGCCAGTTTCTACTGACGATGAGCTG
CTCGAATTCATTGGGGTGGAGAGAAGCCATCCCAGCTGTCACGCGCTGATAGAGCTGATT
GCCCAGCTAGAGGCGCCGGAACTGTTTGAGAAGAATGTGTTTAGTGACACCCGTTCCGAG
GTTCAGGGTATGATCGACAGTGCAGTGTCGAACCACATTGCTCGGCTGTCCAGCAGCCGA
AACTCCCTGAGCATGGACAGCGAGGAATTGGAACGCTTGATTAAATCTTTCCAGATTCAT
ACTCCCCATTGTTCTCTGTTCATAGGCGCTCAGTCCTTATCTCAGCAGCTGGAGAGCTTA
CCTGAGGCGCTGCAGTCCGGAGTGAACAGCGCTGATATCTTATTAGGCAGCACACAGTAT
ATGCTGACCAACTCTCTCGTTGAAGAGTCAATTGCAACATATCAAAGGACATTAAATAGG
ATCAATTACCTGAGTGGGGTGGCTGGGCAGATTAACGGTGCTATCAAAAGAAAGGCAATC
GACGGCGAAAAAATACACCTGCCTGCCGCCTGGAGTGAGCTCATCTCCTTACCTTTCATT
GGACAGCCGGTGATTGATGTGGAGAGCGACCTGGCACACTTAAAAAACCAGTACCAGACC
CTGTCCAATGAATTTGACACCCTCATTTCGGCCCTGCAGAAGAACTTCGATTTGAATTTC
AACAAAGCACTCCTTAACCGCACGCAGCATTTCGAGGCAATGTGCCGGAGCACAAAAAAA
AATGCTTTATCTAAGCCCGAGATCGTGTCCTACAGAGATCTGCTGGCGCGGCTGACCAGT
TGCCTTTATCGAGGCTCGCTGGTTCTCAGAAGGGCGGGAATCGAAGTTCTGAAAAAGCAC
AAAATCTTTGAGTCGAATAGTGAGCTGAGAGAACACGTCCACGAGCGAAAGCACTTCGTG
TTCGTTAGTCCATTGGACAGAAAGGCAAAAAAACTGTTGCGCCTGACCGATTCCCGCCCT
GACTTGCTCCATGTGATCGATGAGATCCTGCAACATGACAATCTGGAGAATAAGGACAGA
GAGTCCCTTTGGCTGGTCCGGTCTGGGTACCTCCTTGCTGGTCTGCCGGACCAGCTGAGT
TCTTCGTTTATCAATCTCCCCATAATCACGCAAAAGGGCGATCGCCGGCTGATTGACCTG
ATTCAGTATGACCAGATCAATCGCGATGCTTTCGTAATGTTGGTGACAAGTGCTTTCAAA
AGCAATCTCTCTGGGTTGCAGTACCGCGCTAACAAGCAGTCTTTCGTGGTCACCCGCACC
CTGTCTCCTTACCTGGGTAGTAAGCTCGTATACGTCCCTAAAGACAAAGATTGGCTGGTC
CCATCCCAGATGTTTGAGGGAAGATTCGCCGATATTCTGCAGAGTGACTACATGGTCTGG
AAGGATGCCGGACGCCTGTGCGTGATCGACACTGCCAAACATCTCTCTAACATTAAAAAA
AGCGTGTTTAGTAGCGAAGAAGTCCTTGCTTTTCTTCGAGAGCTGCCTCACCGGACCTTC
ATCCAGACCGAGGTACGGGGGTTAGGAGTGAACGTCGATGGAATCGCATTTAATAACGGG
GATATCCCGAGCTTGAAGACATTCTCGAATTGTGTGCAGGTGAAGGTGAGTAGGACTAAT
ACTAGTCTCGTGCAGACTCTAAACAGGTGGTTCGAGGGTGGCAAAGTGTCACCTCCCTCT
ATTCAGTTCGAAAGAGCTTACTACAAAAAAGACGATCAGATTCACGAGGACGCAGCCAAG
AGAAAGATACGCTTCCAGATGCCAGCAACGGAATTAGTGCACGCCAGCGATGACGCTGGT
TGGACCCCCAGCTACCTGCTGGGCATCGACCCCGGTGAGTACGGAATGGGTCTCAGTTTG
GTGTCCATCAACAATGGAGAGGTCCTGGATTCTGGATTCATCCACATTAATTCCCTGATC
AATTTCGCGTCCAAAAAAAGCAATCACCAGACCAAAGTAGTCCCCCGCCAGCAGTACAAG
TCCCCCTACGCGAATTATCTCGAGCAGTCAAAGGATTCAGCAGCAGGGGATATAGCTCAC
ATTCTGGATCGGCTAATCTACAAATTGAACGCCTTGCCTGTGTTCGAGGCGCTGTCTGGC
AACAGTCAGAGTGCTGCTGATCAGGTATGGACCAAAGTTCTATCCTTCTATACATGGGGA
GACAACGACGCACAGAACAGTATACGGAAGCAGCACTGGTTCGGTGCCTCACACTGGGAT
ATTAAGGGGATGCTGCGCCAACCCCCAACCGAAAAAAAACCCAAACCATATATAGCCTTT
CCCGGGAGTCAAGTGTCATCCTATGGAAATAGTCAAAGGTGTAGTTGTTGCGGCCGCAAT
CCCATTGAGCAGTTGCGTGAGATGGCAAAGGACACGAGTATCAAGGAGCTGAAAATCCGA
AATAGTGAGATCCAACTATTCGATGGTACAATCAAGCTGTTTAACCCCGACCCTTCCACC
GTCATCGAGAGGCGGCGGCATAACCTAGGACCCTCACGCATTCCTGTGGCAGACCGAACT
TTCAAGAATATTAGCCCTTCTTCGTTAGAGTTCAAGGAGCTCATTACTATCGTTTCTCGA
AGCATCCGCCATAGCCCCGAATTTATTGCTAAGAAACGGGGTATCGGGTCTGAGTACTTT
TGTGCTTATTCTGACTGCAACTCCTCACTGAACTCAGAGGCCAATGCCGCGGCCAATGTG
GCACAGAAGTTTCAGAAGCAACTCTTTTTCGAACTCTGA
SEQ ATGAAACGTATTCTGAACTCTCTGAAAGTCGCCGCACTGAGGCTGCTGTTTCGAGGAAAG
ID GGCTCAGAGCTGGTGAAGACCGTCAAGTACCCTCTGGTTTCGCCCGTCCAGGGTGCTGTG
NO: GAAGAACTCGCCGAAGCAATACGCCACGACAACCTACATTTATTTGGGCAGAAGGAAATC
163 GTAGATCTGATGGAGAAGGACGAGGGCACCCAGGTCTACTCGGTGGTGGACTTTTGGCTC
GACACACTCCGTCTAGGGATGTTCTTCAGTCCAAGTGCTAATGCCCTTAAGATCACTCTG
GGGAAGTTTAACAGCGACCAAGTTTCCCCTTTCAGGAAGGTTCTGGAGCAGTCCCCTTTC
TTTCTCGCGGGTAGACTCAAAGTGGAGCCCGCTGAACGTATCCTCAGCGTGGAGATCCGC
AAGATCGGTAAGAGGGAGAATAGAGTGGAGAACTACGCCGCAGATGTAGAGACTTGTTTT
ATCGGTCAGCTGTCTAGTGATGAAAAGCAGTCTATCCAGAAGCTCGCTAACGATATCTGG
GACTCTAAGGATCACGAAGAGCAAAGGATGCTTAAGGCGGATTTCTTTGCCATTCCCCTC
ATCAAAGACCCAAAGGCAGTGACCGAGGAAGATCCCGAGAATGAAACCGCAGGCAAACAG
AAGCCTCTCGAATTATGTGTGTGCTTAGTGCCCGAGTTGTACACCCGCGGGTTCGGTTCA
ATAGCGGACTTCCTGGTCCAGCGTCTGACACTATTAAGAGACAAAATGAGCACAGACACA
GCAGAAGACTGCCTTGAGTATGTCGGCATAGAGGAGGAGAAGGGTAATGGGATGAACTCG
CTGCTGGGGACGTTCCTCAAGAACCTGCAGGGAGACGGGTTCGAACAGATCTTCCAATTT
ATGCTCGGCAGTTACGTGGGATGGCAAGGTAAGGAAGACGTCCTACGCGAACGGCTTGAT
TTGCTAGCGGAGAAGGTTAAAAGACTGCCGAAACCTAAGTTTGCCGGCGAGTGGTCCGGC
CATCGGATGTTCCTGCATGGTCAATTGAAGAGCTGGTCCTCTAACTTTTTCCGCCTGTTT
AACGAGACTAGGGAGCTCCTCGAAAGCATAAAATCCGACATCCAACACGCGACCATGTTA
ATCAGCTACGTCGAAGAGAAAGGGGGATACCACCCACAACTCTTGTCACAGTACAGGAAA
CTAATGGAGCAGCTGCCAGCTCTCAGAACAAAGGTGTTAGATCCAGAGATAGAAATGACT
CACATGAGCGAGGCGGTAAGGTCGTACATTATGATCCACAAGTCGGTAGCAGGATTTCTG
CCTGACTTACTCGAGTCCCTCGATAGGGACAAGGACAGGGAATTCCTGCTGAGTATATTT
CCAAGGATCCCCAAAATTGACAAAAAAACTAAGGAAATCGTGGCCTGGGAGCTCCCAGGC
GAGCCCGAAGAAGGATACCTGTTCACTGCCAATAATCTTTTTCGCAACTTTCTGGAGAAT
CCTAAACATGTTCCACGTTTCATGGCAGAAAGGATCCCGGAAGATTGGACGCGCCTGCGG
TCCGCTCCCGTATGGTTTGACGGCATGGTGAAACAATGGCAGAAAGTGGTAAACCAGCTG
GTGGAGTCACCTGGAGCATTGTATCAGTTCAATGAAAGCTTTCTCCGACAACGTTTACAG
GCAATGCTGACAGTGTATAAGAGAGACCTGCAGACAGAGAAATTCCTTAAGTTGTTGGCT
GATGTCTGCAGGCCTCTGGTGGACTTCTTTGGGCTGGGGGGAAACGATATCATCTTCAAA
AGCTGCCAGGACCCGAGGAAACAATGGCAAACTGTCATTCCCTTGAGTGTCCCCGCTGAT
GTGTACACCGCGTGTGAGGGGCTGGCAATCCGGCTTCGTGAGACATTGGGATTTGAGTGG
AAGAACCTTAAGGGCCATGAAAGGGAGGACTTTCTAAGACTGCACCAGCTTTTAGGGAAT
CTGCTTTTCTGGATTCGAGATGCCAAACTGGTGGTGAAATTGGAAGATTGGATGAATAAT
CCCTGTGTTCAGGAGTACGTTGAGGCTCGTAAGGCCATTGATCTCCCACTGGAGATCTTC
GGCTTTGAGGTCCCCATCTTCCTGAACGGATATCTGTTTAGTGAACTGAGGCAGTTAGAA
CTGCTGCTCCGCCGTAAGTCGGTTATGACCAGCTATTCGGTTAAGACAACTGGCAGTCCA
AACAGGCTTTTCCAGTTAGTCTACCTGCCATTAAATCCTTCCGACCCTGAGAAAAAAAAT
TCTAATAACTTTCAGGAACGCCTGGACACCCCCACTGGCTTATCACGTCGCTTCCTGGAC
CTTACTCTGGACGCCTTCGCCGGCAAGTTGCTGACAGACCCCGTGACTCAAGAGCTTAAA
ACTATGGCTGGGTTCTACGATCACCTGTTTGGTTTCAAGCTCCCATGTAAGCTGGCAGCC
ATGTCTAACCACCCTGGCTCTAGCAGCAAGATGGTCGTGTTGGCCAAACCTAAAAAAGGG
GTTGCATCTAATATAGGATTCGAACCAATCCCTGATCCCGCGCACCCCGTATTCCGGGTG
AGATCATCATGGCCAGAGCTGAAGTATCTGGAGGGGTTACTGTATCTTCCAGAAGACACT
CCACTGACAATAGAGCTCGCAGAGACAAGTGTTAGTTGTCAGAGCGTCAGTAGCGTGGCA
TTCGATCTGAAAAATCTGACTACTATCCTTGGACGCGTGGGTGAGTTCCGTGTGACCGCA
GACCAGCCTTTTAAGTTGACCCCCATCATCCCTGAGAAGGAGGAGTCCTTCATAGGAAAA
ACATATCTAGGCCTTGATGCCGGGGAACGCTCAGGCGTAGGGTTCGCTATCGTCACAGTC
GACGGGGATGGGTACGAGGTACAGCGCCTGGGGGTGCATGAAGATACACAGCTGATGGCC
CTACAGCAGGTGGCCTCTAAAAGCTTGAAGGAGCCGGTGTTCCAGCCGCTCAGAAAGGGT
ACTTTTCGGCAGCAGGAACGTATTAGAAAATCTCTCAGAGGATGTTATTGGAACTTCTAT
CACGCTCTGATGATTAAGTACCGCGCCAAGGTAGTGCACGAAGAGAGCGTGGGCAGTTCC
GGCCTGGTTGGGCAGTGGTTACGAGCATTCCAGAAGGACCTCAAGAAAGCCGATGTGTTG
CCAAAAAAGGGAGGCAAAAACGGAGTCGATAAGAAAAAGAGAGAGTCTTCTGCACAAGAC
ACATTGTGGGGAGGGGCTTTTAGCAAGAAGGAAGAACAGCAGATAGCTTTCGAAGTCCAA
GCTGCTGGTTCTAGCCAGTTCTGCCTGAAGTGCGGATGGTGGTTCCAACTCGGAATGCGT
GAGGTTAATCGCGTGCAGGAATCCGGCGTCGTGCTGGATTGGAATCGGAGTATTGTCACA
TTCCTGATTGAGAGCTCTGGCGAGAAAGTGTATGGGTTCTCCCCTCAGCAACTCGAAAAG
GGGTTCAGACCAGACATTGAAACCTTCAAGAAGATGGTTCGGGATTTCATGCGCCCGCCT
ATGTTTGACCGGAAGGGTCGCCCAGCAGCTGCCTACGAAAGGTTTGTCTTGGGACGCCGG
CATCGGCGGTATAGATTCGACAAGGTTTTTGAAGAACGATTCGGACGATCCGCGCTATTC
ATTTGCCCGAGGGTTGGCTGTGGCAACTTTGACCACAGCAGCGAGCAGTCAGCCGTAGTG
CTGGCTCTAATCGGATATATTGCCGACAAAGAGGGGATGAGCGGAAAAAAGCTAGTCTAC
GTGCGTCTGGCAGAACTAATGGCGGAATGGAAATTGAAGAAACTGGAGAGGAGTAGAGTT
GAGGAGCAAAGCTCCGCTCAGTGA
SEQ ATGGCGGAGTCGAAGCAAATGCAGTGCAGGAAGTGTGGAGCCTCTATGAAGTACGAAGTG
ID ATCGGCCTCGGGAAGAAAAGCTGCAGATATATGTGTCCCGACTGCGGGAATCACACATCT
NO: GCAAGAAAGATTCAGAATAAGAAGAAAAGGGACAAGAAGTATGGATCTGCCAGTAAAGCA
164 CAAAGCCAACGAATCGCAGTTGCAGGGGCCTTATACCCGGATAAAAAGGTTCAGACCATC
AAGACTTATAAGTATCCAGCCGACCTGAATGGTGAGGTCCATGACTCAGGGGTGGCCGAA
AAAATAGCCCAAGCAATCCAGGAGGATGAAATAGGGCTCCTCGGCCCCTCTTCCGAGTAC
GCCTGTTGGATCGCTAGCCAGAAACAGAGCGAGCCCTACAGTGTTGTAGACTTTTGGTTT
GACGCTGTGTGCGCCGGAGGCGTGTTCGCCTATTCTGGGGCTAGATTGCTGTCTACCGTC
CTGCAGCTATCTGGGGAGGAGAGCGTCCTACGCGCAGCCCTGGCATCCTCCCCTTTTGTC
GACGATATCAATCTGGCACAGGCCGAAAAATTTCTGGCGGTGTCCAGGCGAACCGGCCAA
GATAAGCTGGGGAAGCGCATTGGAGAGTGCTTCGCAGAGGGCCGACTTGAGGCCCTAGGC
ATCAAGGACCGGATGCGTGAATTTGTCCAGGCTATCGATGTCGCTCAGACCGCTGGGCAG
CGTTTTGCCGCGAAACTGAAAATCTTTGGGATTTCTCAGATGCCCGAGGCAAAGCAGTGG
AACAATGACAGCGGACTCACCGTGTGCATCCTGCCCGACTATTACGTCCCAGAAGAAAAT
CGCGCAGATCAGTTGGTCGTCCTGCTAAGACGACTGAGAGAGATAGCATACTGTATGGGG
ATCGAAGATGAGGCCGGTTTTGAACATCTTGGAATTGATCCTGGCGCACTATCAAATTTT
TCCAATGGCAATCCTAAACGCGGATTTTTGGGCCGCCTGCTGAACAATGATATTATTGCC
TTAGCGAACAACATGTCCGCCATGACGCCTTACTGGGAGGGCAGGAAGGGAGAACTGATT
GAAAGATTGGCTTGGCTGAAGCACCGTGCAGAGGGGCTTTATCTGAAGGAACCGCATTTT
GGAAATAGTTGGGCCGACCATAGGTCTAGAATTTTTTCCAGAATAGCCGGGTGGCTTTCT
GGGTGCGCTGGGAAGCTAAAGATCGCCAAAGACCAGATCAGCGGAGTGCGTACTGATCTG
TTCCTTCTGAAGAGACTGCTGGATGCGGTCCCGCAGTCCGCCCCTTCTCCCGACTTCATA
GCCTCTATCTCTGCCTTGGATCGCTTCCTGGAGGCCGCAGAATCTAGTCAGGATCCTGCC
GAACAGGTGAGGGCCCTATACGCCTTTCATCTGAACGCACCCGCGGTGCGAAGCATCGCC
AACAAGGCAGTCCAGCGATCCGACAGCCAAGAATGGCTTATAAAGGAACTGGACGCTGTG
GACCACCTGGAGTTTAACAAGGCCTTTCCCTTCTTCTCTGATACGGGAAAGAAGAAAAAG
AAAGGGGCTAACTCGAATGGCGCTCCGTCCGAGGAGGAGTACACCGAGACTGAGAGCATC
CAGCAGCCCGAGGACGCTGAGCAAGAGGTTAATGGTCAGGAAGGCAACGGGGCCTCGAAG
AACCAGAAGAAGTTTCAGAGAATCCCCCGATTCTTCGGCGAGGGGAGTCGCAGCGAGTAT
CGCATCCTCACTGAAGCCCCGCAGTACTTCGACATGTTCTGTAACAACATGCGGGCCATC
TTTATGCAATTAGAATCCCAACCGCGTAAAGCTCCCAGGGATTTTAAGTGTTTCCTGCAG
AATCGGCTGCAGAAATTGTATAAGCAGACATTCCTGAACGCTCGATCCAACAAGTGCCGG
GCATTACTAGAGTCCGTATTGATTAGTTGGGGAGAGTTTTACACCTACGGGGCTAACGAG
AAAAAATTTCGACTGCGTCATGAAGCTTCTGAGCGCTCCTCGGACCCAGATTACGTGGTG
CAACAGGCGCTGGAGATCGCTCGGAGGCTGTTTCTCTTCGGCTTTGAGTGGAGGGACTGT
AGCGCAGGTGAAAGAGTGGATCTGGTCGAAATACATAAGAAAGCCATATCTTTCCTGTTG
GCCATCACTCAGGCTGAGGTGTCTGTGGGCAGCTATAACTGGCTGGGCAATTCTACCGTG
AGTCGGTACCTGTCCGTGGCAGGGACTGATACCCTTTACGGCACCCAGCTGGAAGAATTC
TTAAATGCAACCGTGTTATCTCAGATGCGGGGGCTGGCTATCAGGTTATCATCTCAGGAA
CTGAAGGATGGATTTGACGTACAGCTGGAGTCTAGTTGCCAGGATAATCTGCAACACTTG
CTCGTGTACAGGGCTTCACGAGACCTTGCCGCCTGCAAGCGCGCTACTTGTCCAGCTGAG
TTGGATCCTAAGATTCTGGTACTGCCCGTGGGGGCCTTTATCGCTAGCGTGATGAAAATG
ATTGAAAGAGGGGATGAGCCTTTAGCTGGAGCTTATCTGAGACACAGACCCCATAGTTTC
GGGTGGCAGATCCGCGTTCGAGGTGTGGCAGAGGTGGGAATGGACCAAGGGACCGCCCTG
GCGTTCCAGAAACCGACCGAGAGCGAACCCTTCAAGATAAAGCCGTTTTCCGCTCAATAC
GGCCCCGTTCTATGGCTGAACAGCTCCAGTTATAGCCAGAGCCAGTACCTGGACGGGTTC
CTATCACAGCCCAAGAACTGGAGTATGCGGGTGCTGCCACAGGCCGGCTCAGTGCGGGTA
GAACAGCGCGTCGCCTTGATTTGGAATCTCCAGGCCGGAAAGATGAGGCTGGAACGGAGC
GGAGCGCGGGCTTTCTTCATGCCCGTCCCATTCAGTTTCCGCCCCAGTGGCAGCGGCGAC
GAGGCAGTCCTGGCTCCAAATAGGTACCTGGGACTCTTTCCACACAGCGGCGGCATAGAG
TACGCTGTGGTCGATGTTCTTGACTCTGCCGGCTTCAAAATACTCGAGAGAGGAACAATA
GCCGTCAATGGCTTCTCCCAGAAACGAGGAGAAAGACAAGAGGAAGCCCATCGCGAAAAA
CAAAGACGCGGTATCTCCGATATTGGGCGCAAGAAGCCAGTCCAGGCCGAAGTCGATGCG
GCCAACGAGCTCCATCGAAAATACACCGATGTTGCTACTCGGCTGGGGTGTCGAATTGTC
GTTCAATGGGCACCCCAACCCAAACCAGGCACTGCGCCGACCGCTCAGACTGTGTACGCT
AGGGCCGTGAGGACTGAAGCACCAAGATCCGGCAATCAGGAAGATCACGCCAGGATGAAA
TCTTCCTGGGGATACACATGGGGTACGTATTGGGAAAAAAGGAAGCCCGAGGACATCCTC
GGCATTAGTACCCAGGTGTATTGGACAGGCGGGATCGGCGAGTCCTGCCCGGCTGTCGCC
GTCGCGCTATTGGGACACATCAGGGCCACCTCAACCCAGACTGAATGGGAGAAAGAGGAA
GTCGTGTTTGGGCGATTGAAAAAGTTCTTCCCATCCTGA
SEQ ATGGAGAAGCGCATCAATAAAATTCGCAAGAAGCTGTCTGCCGATAACGCCACAAAACCA
ID GTTAGTCGAAGCGGCCCAATGAAGACCCTGCTAGTTCGAGTGATGACTGATGATCTGAAG
NO: AAAAGGCTCGAAAAGCGACGCAAGAAGCCTGAGGTAATGCCTCAGGTTATAAGTAACAAT
165 GCAGCAAACAATCTGCGGATGCTGCTTGACGATTACACAAAGATGAAGGAAGCCATTCTC
CAGGTGTATTGGCAGGAGTTCAAGGATGATCACGTAGGCCTGATGTGTAAATTCGCGCAA
CCTGCAAGCAAGAAGATCGACCAAAACAAGCTGAAACCCGAGATGGATGAAAAAGGCAAT
TTAACAACCGCCGGATTCGCTTGTTCCCAGTGTGGGCAGCCACTGTTCGTGTACAAGTTA
GAACAGGTGTCGGAAAAAGGAAAGGCATACACTAACTACTTTGGACGGTGCAATGTTGCA
GAACACGAAAAGCTGATACTGCTTGCCCAGCTTAAGCCCGAAAAAGACAGCGACGAAGCG
GTGACCTACAGCCTGGGAAAATTCGGGCAGCGGGCACTGGACTTCTATTCTATCCACGTT
ACCAAGGAGAGCACCCACCCAGTGAAGCCGTTGGCCCAAATCGCTGGAAACCGGTACGCC
AGCGGACCAGTCGGCAAGGCCCTGTCCGATGCCTGTATGGGCACAATTGCTTCTTTCCTG
TCCAAGTACCAGGACATCATAATCGAGCACCAAAAAGTTGTGAAAGGGAATCAGAAACGC
CTGGAATCCCTTCGAGAACTGGCCGGCAAGGAGAACCTTGAGTACCCGTCCGTGACCCTG
CCTCCACAGCCACATACCAAAGAGGGCGTAGACGCGTATAATGAGGTCATTGCCCGCGTT
CGCATGTGGGTTAATTTAAACCTGTGGCAGAAATTAAAACTAAGCCGAGATGATGCTAAA
CCGTTACTGAGATTGAAGGGATTCCCTAGCTTTCCTGTGGTGGAGAGAAGGGAAAACGAG
GTTGATTGGTGGAATACTATTAATGAGGTGAAAAAGCTTATTGACGCCAAGAGGGATATG
GGCAGGGTGTTCTGGAGCGGGGTGACTGCCGAAAAGAGAAATACCATCCTCGAGGGATAC
AATTACCTCCCCAACGAGAATGATCATAAGAAAAGAGAGGGGAGCTTAGAGAATCCAAAG
AAACCTGCAAAGAGGCAATTCGGTGATCTCCTGCTCTACCTCGAGAAGAAATACGCGGGG
GACTGGGGAAAAGTTTTTGACGAAGCCTGGGAGCGCATTGACAAGAAGATCGCCGGGCTG
ACGTCTCACATTGAACGGGAAGAGGCACGGAATGCAGAGGACGCCCAGTCTAAGGCCGTG
CTGACTGACTGGCTGCGCGCAAAGGCCTCCTTCGTGCTCGAACGTCTGAAGGAAATGGAT
GAGAAAGAGTTTTACGCGTGTGAAATACAGCTGCAGAAGTGGTACGGCGATCTAAGGGGA
AATCCCTTCGCAGTGGAAGCCGAGAATAGGGTAGTTGACATCAGTGGGTTCTCCATCGGC
AGTGATGGACATTCTATCCAGTATAGAAACCTGCTCGCCTGGAAGTACTTAGAGAACGGC
AAGAGAGAGTTCTATCTGCTGATGAACTACGGGAAAAAAGGTAGAATTCGCTTTACAGAT
GGCACCGACATAAAGAAGTCCGGAAAGTGGCAAGGCCTCTTATACGGAGGCGGCAAAGCA
AAGGTGATAGACTTGACTTTTGACCCTGACGACGAACAGCTGATAATCTTGCCGCTGGCC
TTTGGCACAAGACAAGGTAGGGAATTTATCTGGAATGATCTTCTTTCTCTCGAGACCGGA
CTCATCAAGCTCGCAAACGGAAGGGTCATCGAGAAGACAATCTACAATAAAAAGATAGGC
CGAGACGAGCCAGCCCTGTTTGTGGCTTTGACATTTGAGCGGAGAGAGGTCGTAGATCCC
AGCAACATCAAACCCGTGAACCTGATCGGTGTTGACAGGGGCGAGAACATCCCGGCGGTT
ATCGCACTGACGGATCCAGAAGGATGTCCTCTGCCCGAGTTCAAAGATTCATCGGGAGGG
CCAACCGACATTTTGAGGATAGGGGAGGGGTACAAGGAGAAGCAGCGAGCTATCCAGGCG
GCCAAAGAAGTGGAGCAACGAAGAGCTGGTGGTTATTCTCGCAAGTTCGCTTCCAAAAGT
CGTAACCTGGCTGACGATATGGTGCGCAATTCTGCCCGTGACCTTTTCTACCACGCCGTT
ACACACGACGCCGTGTTAGTGTTTGAAAATCTTAGTCGAGGCTTCGGGCGACAGGGGAAG
CGGACCTTTATGACCGAGAGACAGTATACAAAAATGGAGGATTGGCTGACCGCCAAACTG
GCGTATGAAGGACTCACATCCAAGACCTATCTCTCAAAAACTTTGGCCCAGTATACATCT
AAGACGTGCAGTAACTGTGGCTTCACCATTACCACAGCTGACTACGATGGCATGCTGGTC
CGCTTAAAAAAGACATCTGACGGCTGGGCTACTACCCTCAACAATAAAGAGCTCAAAGCC
GAAGGACAAATTACCTATTATAACAGGTATAAAAGACAGACTGTCGAGAAGGAGTTGAGC
GCGGAGCTGGACCGCCTATCAGAGGAGTCAGGGAACAACGATATCTCTAAGTGGACTAAG
GGACGCCGAGACGAGGCGTTGTTCTTGCTGAAAAAGCGGTTCTCTCATCGACCCGTGCAG
GAGCAGTTCGTGTGTCTGGACTGCGGCCACGAGGTTCATGCTGATGAGCAAGCTGCTCTA
AATATTGCCCGTAGTTGGTTGTTCCTGAACAGCAATTCAACAGAGTTCAAGTCATACAAG
AGCGGAAAGCAGCCGTTTGTGGGCGCATGGCAGGCATTTTACAAAAGACGCCTGAAGGAA
GTGTGGAAGCCAAACGCC
SEQ ATGAAAAGGATTAACAAAATCCGAAGGCGGCTTGTAAAGGATTCTAACACCAAAAAGGCT
ID GGCAAGACGGGGCCCATGAAAACATTACTCGTTAGAGTTATGACCCCCGACCTCAGAGAG
NO: CGACTGGAAAATTTACGCAAGAAGCCAGAGAACATACCTCAGCCAATTAGTAATACCTCT
166 CGGGCAAACCTAAACAAGTTGCTTACTGATTACACGGAGATGAAAAAGGCCATACTGCAT
GTGTACTGGGAGGAGTTTCAAAAGGACCCTGTCGGGCTAATGAGCAGGGTGGCTCAGCCT
GCACCTAAAAACATCGACCAGCGGAAACTCATCCCAGTTAAGGACGGAAATGAGAGATTG
ACAAGTTCAGGTTTCGCCTGCTCACAGTGCTGTCAACCGCTGTACGTTTATAAGTTAGAA
CAAGTGAATGACAAAGGAAAGCCTCACACAAATTATTTTGGCCGGTGTAATGTCTCTGAG
CATGAGCGTCTGATTCTGTTGTCCCCGCATAAACCGGAAGCTAATGACGAGCTCGTAACC
TACAGCTTGGGGAAGTTTGGCCAAAGAGCATTGGACTTCTATTCAATCCATGTGACCCGC
GAATCCAATCATCCCGTCAAGCCCTTGGAGCAGATAGGGGGCAATAGTTGCGCTTCTGGC
CCTGTGGGCAAAGCCCTGTCCGACGCCTGTATGGGAGCCGTGGCTTCATTCCTGACCAAA
TATCAGGATATCATCTTGGAGCACCAGAAAGTGATCAAGAAAAATGAAAAAAGGTTAGCA
AACCTCAAGGATATTGCAAGCGCTAACGGCTTGGCTTTTCCTAAAATCACACTTCCACCT
CAGCCTCACACAAAGGAAGGCATCGAGGCATACAACAATGTGGTGGCCCAGATCGTCATC
TGGGTTAACTTAAACCTGTGGCAGAAACTTAAAATTGGCAGGGATGAGGCAAAACCCTTA
CAGCGCCTGAAAGGATTCCCCAGCTTTCCACTGGTGGAGCGCCAGGCTAACGAAGTGGAC
TGGTGGGATATGGTGTGTAACGTCAAGAAGCTCATCAATGAAAAGAAAGAGGACGGTAAA
GTCTTCTGGCAGAACCTCGCCGGTTACAAACGGCAGGAGGCGCTGTTACCTTATCTGTCG
AGTGAAGAGGACCGGAAAAAAGGCAAGAAATTTGCTCGTTATCAGTTTGGTGATTTGCTC
CTACATTTGGAGAAGAAGCACGGCGAGGACTGGGGAAAAGTATACGATGAGGCCTGGGAG
AGGATTGACAAAAAGGTGGAGGGACTGTCAAAGCACATCAAGCTCGAAGAAGAGCGCAGA
AGCGAGGACGCCCAATCCAAAGCAGCGCTGACTGACTGGCTGCGGGCGAAGGCCAGTTTT
GTAATCGAAGGCCTTAAAGAAGCCGACAAGGATGAATTCTGCAGATGCGAATTAAAACTC
CAGAAGTGGTACGGCGATCTCCGAGGTAAGCCTTTCGCAATCGAGGCCGAGAATTCCATA
CTGGACATTAGTGGATTCAGTAAACAGTATAATTGTGCCTTTATATGGCAGAAGGATGGT
GTCAAGAAACTCAACCTGTACCTTATTATTAATTATTTCAAAGGCGGGAAACTGAGATTT
AAGAAGATAAAGCCTGAAGCCTTTGAGGCGAACCGATTCTACACAGTTATTAACAAGAAA
TCTGGTGAAATTGTACCCATGGAGGTAAACTTCAACTTCGATGATCCCAATCTGATTATA
TTGCCACTAGCTTTTGGCAAGCGGCAGGGTAGGGAATTCATTTGGAACGATTTGCTTTCA
CTGGAAACAGGGTCCCTTAAGCTGGCAAACGGGAGAGTGATTGAAAAGACATTGTACAAT
CGGAGGACACGTCAGGATGAACCTGCCCTTTTCGTGGCTCTGACATTCGAGCGCAGGGAG
GTTCTGGACTCTAGCAATATCAAGCCAATGAACCTGATCGGCATAGACCGAGGAGAGAAT
ATTCCGGCTGTGATCGCACTCACCGATCCCGAAGGATGTCCCCTTTCTCGGTTCAAGGAC
TCCTTAGGCAATCCAACTCATATCCTGAGAATCGGCGAGTCATACAAGGAGAAGCAGCGA
ACAATTCAGGCCGCCAAGGAAGTCGAGCAGAGGCGAGCTGGCGGCTACAGCCGTAAATAC
GCTAGTAAAGCTAAGAACCTGGCCGACGATATGGTGCGCAATACTGCTAGAGACCTGCTG
TACTATGCAGTGACGCAGGACGCAATGCTGATATTCGAGAATCTGTCCAGAGGATTCGGA
AGGCAGGGCAAGCGGACGTTCATGGCCGAGCGCCAGTATACAAGGATGGAGGATTGGTTA
ACGGCCAAGCTTGCCTATGAGGGGCTACCTAGTAAGACCTATCTGTCTAAGACGCTGGCT
CAATACACCAGTAAGACCTGCTCAAACTGTGGCTTTACAATCACTTCTGCTGATTATGAT
AGAGTGCTCGAGAAGCTAAAAAAAACTGCCACCGGCTGGATGACTACTATTAATGGGAAG
GAACTGAAAGTGGAAGGACAGATTACCTATTATAATCGCTACAAGCGTCAAAACGTCGTC
AAGGACCTGTCGGTGGAATTGGACAGACTCAGTGAAGAGTCCGTGAACAATGATATCAGC
TCCTGGACAAAAGGGCGCAGTGGGGAGGCACTCAGCTTGCTTAAAAAGAGGTTTTCACAT
CGGCCGGTCCAGGAGAAATTTGTCTGCCTGAACTGCGGATTCGAGACACACGCCGACGAG
CAGGCAGCACTGAACATTGCCAGATCCTGGCTGTTCCTTAGGTCCCAGGAATATAAGAAG
TACCAGACTAACAAAACCACGGGAAACACAGATAAAAGGGCCTTTGTCGAAACTTGGCAA
TCCTTTTACCGGAAGAAGTTAAAGGAAGTGTGGAAGCCC
SEQ ATGGATAAGAAATACTCAATAGGCTTAGCAATCGGCACAAATAGCGTCGGATGGGCGGTG
ID ATCACTGATGAATATAAGGTTCCGTCTAAAAAGTTCAAGGTTCTGGGAAATACAGACCGC
NO: CACAGTATCAAAAAAAATCTTATAGGGGCTCTTTTATTTGACAGTGGAGAGACAGCGGAA
167 GCGACTCGTCTCAAACGGACAGCTCGTAGAAGGTATACACGTCGGAAGAATCGTATTTGT
TATCTACAGGAGATTTTTTCAAATGAGATGGCGAAAGTAGATGATAGTTTCTTTCATCGA
CTTGAAGAGTCTTTTTTGGTGGAAGAAGACAAGAAGCATGAACGTCATCCTATTTTTGGA
AATATAGTAGATGAAGTTGCTTATCATGAGAAATATCCAACTATCTATCATCTGCGAAAA
AAATTGGTAGATTCTACTGATAAAGCGGATTTGCGCTTAATCTATTTGGCCTTAGCGCAT
ATGATTAAGTTTCGTGGTCATTTTTTGATTGAGGGAGATTTAAATCCTGATAATAGTGAT
GTGGACAAACTATTTATCCAGTTGGTACAAACCTACAATCAATTATTTGAAGAAAACCCT
ATTAACGCAAGTGGAGTAGATGCTAAAGCGATTCTTTCTGCACGATTGAGTAAATCAAGA
CGATTAGAAAATCTCATTGCTCAGCTCCCCGGTGAGAAGAAAAATGGCTTATTTGGGAAT
CTCATTGCTTTGTCATTGGGTTTGACCCCTAATTTTAAATCAAATTTTGATTTGGCAGAA
GATGCTAAATTACAGCTTTCAAAAGATACTTACGATGATGATTTAGATAATTTATTGGCG
CAAATTGGAGATCAATATGCTGATTTGTTTTTGGCAGCTAAGAATTTATCAGATGCTATT
TTACTTTCAGATATCCTAAGAGTAAATACTGAAATAACTAAGGCTCCCCTATCAGCTTCA
ATGATTAAACGCTACGATGAACATCATCAAGACTTGACTCTTTTAAAAGCTTTAGTTCGA
CAACAACTTCCAGAAAAGTATAAAGAAATCTTTTTTGATCAATCAAAAAACGGATATGCA
GGTTATATTGATGGGGGAGCTAGCCAAGAAGAATTTTATAAATTTATCAAACCAATTTTA
GAAAAAATGGATGGTACTGAGGAATTATTGGTGAAACTAAATCGTGAAGATTTGCTGCGC
AAGCAACGGACCTTTGACAACGGCTCTATTCCCCATCAAATTCACTTGGGTGAGCTGCAT
GCTATTTTGAGAAGACAAGAAGACTTTTATCCATTTTTAAAAGACAATCGTGAGAAGATT
GAAAAAATCTTGACTTTTCGAATTCCTTATTATGTTGGTCCATTGGCGCGTGGCAATAGT
CGTTTTGCATGGATGACTCGGAAGTCTGAAGAAACAATTACCCCATGGAATTTTGAAGAA
GTTGTCGATAAAGGTGCTTCAGCTCAATCATTTATTGAACGCATGACAAACTTTGATAAA
AATCTTCCAAATGAAAAAGTACTACCAAAACATAGTTTGCTTTATGAGTATTTTACGGTT
TATAACGAATTGACAAAGGTCAAATATGTTACTGAAGGAATGCGAAAACCAGCATTTCTT
TCAGGTGAACAGAAGAAAGCCATTGTTGATTTACTCTTCAAAACAAATCGAAAAGTAACC
GTTAAGCAATTAAAAGAAGATTATTTCAAAAAAATAGAATGTTTTGATAGTGTTGAAATT
TCAGGAGTTGAAGATAGATTTAATGCTTCATTAGGTACCTACCATGATTTGCTAAAAATT
ATTAAAGATAAAGATTTTTTGGATAATGAAGAAAATGAAGATATCTTAGAGGATATTGTT
TTAACATTGACCTTATTTGAAGATAGGGAGATGATTGAGGAAAGACTTAAAACATATGCT
CACCTCTTTGATGATAAGGTGATGAAACAGCTTAAACGTCGCCGTTATACTGGTTGGGGA
CGTTTGTCTCGAAAATTGATTAATGGTATTAGGGATAAGCAATCTGGCAAAACAATATTA
GATTTTTTGAAATCAGATGGTTTTGCCAATCGCAATTTTATGCAGCTGATCCATGATGAT
AGTTTGACATTTAAAGAAGACATTCAAAAAGCACAAGTGTCTGGACAAGGCGATAGTTTA
CATGAACATATTGCAAATTTAGCTGGTAGCCCTGCTATTAAAAAAGGTATTTTACAGACT
GTAAAAGTTGTTGATGAATTGGTCAAAGTAATGGGGCGGCATAAGCCAGAAAATATCGTT
ATTGAAATGGCACGTGAAAATCAGACAACTCAAAAGGGCCAGAAAAATTCGCGAGAGCGT
ATGAAACGAATCGAAGAAGGTATCAAAGAATTAGGAAGTCAGATTCTTAAAGAGCATCCT
GTTGAAAATACTCAATTGCAAAATGAAAAGCTCTATCTCTATTATCTCCAAAATGGAAGA
GACATGTATGTGGACCAAGAATTAGATATTAATCGTTTAAGTGATTATGATGTCGATCAC
ATTGTTCCACAAAGTTTCCTTAAAGACGATTCAATAGACAATAAGGTCTTAACGCGTTCT
GATAAAAATCGTGGTAAATCGGATAACGTTCCAAGTGAAGAAGTAGTCAAAAAGATGAAA
AACTATTGGAGACAACTTCTAAACGCCAAGTTAATCACTCAACGTAAGTTTGATAATTTA
ACGAAAGCTGAACGTGGAGGTTTGAGTGAACTTGATAAAGCTGGTTTTATCAAACGCCAA
TTGGTTGAAACTCGCCAAATCACTAAGCATGTGGCACAAATTTTGGATAGTCGCATGAAT
ACTAAATACGATGAAAATGATAAACTTATTCGAGAGGTTAAAGTGATTACCTTAAAATCT
AAATTAGTTTCTGACTTCCGAAAAGATTTCCAATTCTATAAAGTACGTGAGATTAACAAT
TACCATCATGCCCATGATGCGTATCTAAATGCCGTCGTTGGAACTGCTTTGATTAAGAAA
TATCCAAAACTTGAATCGGAGTTTGTCTATGGTGATTATAAAGTTTATGATGTTCGTAAA
ATGATTGCTAAGTCTGAGCAAGAAATAGGCAAAGCAACCGCAAAATATTTCTTTTACTCT
AATATCATGAACTTCTTCAAAACAGAAATTACACTTGCAAATGGAGAGATTCGCAAACGC
CCTCTAATCGAAACTAATGGGGAAACTGGAGAAATTGTCTGGGATAAAGGGCGAGATTTT
GCCACAGTGCGCAAAGTATTGTCCATGCCCCAAGTCAATATTGTCAAGAAAACAGAAGTA
CAGACAGGCGGATTCTCCAAGGAGTCAATTTTACCAAAAAGAAATTCGGACAAGCTTATT
GCTCGTAAAAAAGACTGGGATCCAAAAAAATATGGTGGTTTTGATAGTCCAACGGTAGCT
TATTCAGTCCTAGTGGTTGCTAAGGTGGAAAAAGGGAAATCGAAGAAGTTAAAATCCGTT
AAAGAGTTACTAGGGATCACAATTATGGAAAGAAGTTCCTTTGAAAAAAATCCGATTGAC
TTTTTAGAAGCTAAAGGATATAAGGAAGTTAAAAAAGACTTAATCATTAAACTACCTAAA
TATAGTCTTTTTGAGTTAGAAAACGGTCGTAAACGGATGCTGGCTAGTGCCGGAGAATTA
CAAAAAGGAAATGAGCTGGCTCTGCCAAGCAAATATGTGAATTTTTTATATTTAGCTAGT
CATTATGAAAAGTTGAAGGGTAGTCCAGAAGATAACGAACAAAAACAATTGTTTGTGGAG
CAGCATAAGCATTATTTAGATGAGATTATTGAGCAAATCAGTGAATTTTCTAAGCGTGTT
ATTTTAGCAGATGCCAATTTAGATAAAGTTCTTAGTGCATATAACAAACATAGAGACAAA
CCAATACGTGAACAAGCAGAAAATATTATTCATTTATTTACGTTGACGAATCTTGGAGCT
CCCGCTGCTTTTAAATATTTTGATACAACAATTGATCGTAAACGATATACGTCTACAAAA
GAAGTTTTAGATGCCACTCTTATCCATCAATCCATCACTGGTCTTTATGAAACACGCATT
GATTTGAGTCAGCTAGGAGGTGACTGA
SEQ ATGGATAAGAAGTATTCAATTGGACTTGCGATTGGCACTAACAGTGTGGGCTGGGCGGTG
ID ATTACAGACGAGTATAAGGTGCCGTCAAAAAAGTTTAAAGTTCTGGGCAACACTGATCGC
NO: CATTCCATCAAGAAAAACCTAATCGGGGCCCTTCTTTTTGATAGTGGCGAAACGGCCGAG
168 GCGACGCGTCTAAAACGTACCGCGCGGCGTCGCTACACCCGACGAAAAAACCGTATTTGT
TACCTTCAGGAGATCTTCAGTAACGAAATGGCTAAGGTGGACGATTCATTCTTCCACCGT
CTGGAGGAGTCCTTTTTAGTTGAAGAAGACAAGAAGCATGAGCGACACCCAATTTTTGGT
AACATTGTCGACGAAGTCGCCTATCACGAAAAATATCCGACCATTTATCACCTGCGCAAA
AAACTGGTCGATAGCACGGATAAAGCGGATCTGCGGCTTATTTACCTGGCGCTTGCCCAC
ATGATCAAGTTCCGCGGCCACTTCCTGATAGAAGGAGACCTGAACCCGGATAATAGCGAT
GTAGACAAACTGTTTATTCAGCTGGTCCAGACCTACAACCAGCTGTTTGAAGAAAATCCG
ATTAATGCGTCAGGCGTGGATGCGAAAGCGATACTGAGTGCCCGCCTGTCGAAATCTCGC
CGTCTCGAAAATCTGATTGCACAGCTGCCCGGCGAAAAAAAAAACGGTCTTTTTGGCAAT
CTGATCGCGCTGTCACTGGGCCTGACACCAAATTTTAAGAGCAACTTCGACCTGGCAGAG
GATGCGAAGCTTCAACTGTCGAAGGACACCTATGACGATGATCTGGATAATCTTCTGGCA
CAAATCGGTGATCAGTATGCGGATTTATTCCTTGCAGCGAAAAACCTATCTGACGCAATT
CTGTTGAGCGATATCCTCCGCGTCAACACCGAAATCACTAAAGCCCCCCTGTCAGCGTCG
ATGATTAAACGTTATGATGAGCACCATCAGGATCTGACCTTGCTAAAGGCGCTGGTGCGA
CAGCAGCTTCCCGAAAAATATAAAGAGATCTTTTTTGATCAATCGAAGAATGGTTATGCC
GGATACATTGATGGCGGAGCCAGTCAGGAAGAATTTTACAAATTCATCAAACCGATCCTG
GAAAAAATGGATGGCACAGAAGAACTGCTTGTGAAATTGAACCGGGAAGATTTACTGCGC
AAACAGCGTACGTTCGACAACGGCTCCATACCCCATCAGATTCACTTAGGTGAGCTGCAT
GCAATACTCCGTCGCCAGGAAGATTTTTATCCATTTTTAAAAGACAACCGTGAGAAGATT
GAAAAAATTTTAACTTTTCGTATTCCATATTACGTCGGGCCTTTGGCCCGAGGTAACTCT
CGATTCGCCTGGATGACGAGAAAAAGCGAGGAGACCATCACTCCGTGGAATTTTGAAGAG
GTTGTTGATAAAGGCGCGAGCGCCCAGTCGTTTATCGAACGTATGACCAACTTTGATAAA
AATCTGCCGAATGAAAAAGTGCTTCCGAAGCATTCTCTGTTGTATGAATATTTCACTGTG
TACAATGAGTTAACGAAAGTGAAATATGTGACCGAAGGCATGCGGAAACCTGCTTTTCTG
TCCGGAGAACAGAAAAAAGCAATTGTGGACCTGCTGTTCAAAACGAACCGGAAAGTAACT
GTGAAGCAGCTGAAAGAGGACTACTTCAAAAAAATCGAATGCTTCGACTCAGTAGAGATC
TCTGGTGTTGAAGATCGCTTCAACGCGAGTCTGGGAACGTACCATGATTTGTTGAAAATC
ATCAAAGATAAAGACTTTCTGGATAACGAAGAGAATGAGGACATTCTTGAAGATATTGTT
TTGACACTGACTCTGTTTGAGGATCGCGAAATGATTGAAGAGCGCCTGAAAACGTATGCC
CATTTATTCGATGACAAAGTCATGAAGCAGCTGAAACGTCGCCGCTATACTGGGTGGGGC
AGACTTTCACGTAAATTGATCAATGGTATAAGAGACAAACAGAGCGGCAAAACTATCTTA
GATTTCCTGAAGAGTGATGGATTTGCCAACCGGAATTTTATGCAGCTTATACATGATGAC
TCGCTAACGTTTAAAGAAGACATTCAGAAGGCGCAGGTCAGCGGCCAGGGTGATTCGCTG
CATGAACACATTGCAAATCTTGCCGGATCGCCAGCGATCAAAAAAGGCATCCTTCAGACA
GTAAAAGTTGTGGATGAACTGGTGAAAGTAATGGGTCGTCACAAGCCAGAAAATATTGTG
ATCGAAATGGCCCGGGAAAATCAGACTACTCAAAAAGGTCAGAAAAATTCTCGCGAGCGT
ATGAAACGTATTGAAGAAGGCATCAAAGAGCTAGGCAGCCAGATATTAAAGGAACATCCG
GTTGAGAACACTCAGCTGCAGAATGAAAAACTGTATCTGTATTATCTTCAGAACGGCCGT
GACATGTATGTTGATCAAGAACTGGATATCAATCGCTTGTCCGATTATGACGTGGATCAT
ATTGTTCCGCAAAGCTTTCTGAAAGACGATTCTATTGACAATAAAGTACTGACACGTTCG
GACAAAAACCGTGGTAAAAGCGATAACGTACCGTCGGAAGAAGTTGTTAAGAAAATGAAA
AATTATTGGCGCCAACTCCTGAATGCTAAATTGATTACCCAGCGGAAATTTGATAACTTA
ACCAAAGCCGAGCGGGGTGGCTTAAGTGAACTGGATAAAGCGGGTTTTATTAAACGCCAA
CTGGTAGAAACCCGCCAGATAACGAAACATGTAGCTCAAATCCTCGATAGTCGCATGAAT
ACGAAATATGACGAAAATGATAAATTGATCCGTGAAGTAAAAGTGATTACTCTTAAAAGC
AAATTGGTATCTGATTTTCGGAAAGATTTCCAATTCTATAAGGTGAGAGAAATTAACAAT
TACCATCATGCACATGATGCGTATTTAAATGCAGTTGTTGGCACCGCCTTAATCAAAAAA
TATCCGAAATTAGAATCTGAGTTCGTGTATGGTGATTATAAAGTTTATGATGTTCGAAAA
ATGATTGCTAAGTCTGAACAGGAAATCGGCAAAGCGACCGCAAAGTATTTTTTTTATAGC
AATATTATGAATTTTTTTAAAACTGAGATTACCCTGGCGAATGGCGAAATTCGCAAACGT
CCTCTGATTGAAACCAATGGCGAAACCGGCGAGATAGTATGGGACAAGGGCCGTGATTTT
GCGACCGTCCGGAAAGTCCTGTCAATGCCGCAGGTGAATATTGTCAAGAAAACAGAAGTT
CAGACAGGCGGTTTTAGTAAAGAGTCTATTCTGCCCAAACGTAATTCGGATAAATTGATT
GCCCGCAAGAAAGATTGGGATCCGAAGAAATATGGTGGATTCGATTCTCCGACGGTCGCC
TATAGCGTTCTAGTCGTCGCCAAGGTCGAAAAAGGTAAATCCAAAAAACTGAAATCTGTG
AAAGAACTGTTAGGCATTACAATCATGGAACGTAGTAGTTTTGAAAAGAACCCGATCGAC
TTCCTCGAGGCGAAAGGCTACAAAGAAGTCAAGAAGGATTTGATTATTAAACTCCCAAAA
TATTCATTATTTGAGTTAGAAAACGGTAGGAAGCGTATGCTGGCGAGTGCTGGGGAATTA
CAGAAAGGGAATGAGTTAGCACTGCCGTCAAAATATGTGAACTTTCTGTATCTGGCCTCC
CATTACGAGAAACTGAAAGGTAGCCCGGAAGATAATGAACAGAAACAACTATTTGTCGAG
CAACACAAACATTATCTGGATGAAATTATTGAACAGATTAGTGAATTCTCTAAACGTGTT
ATTTTAGCGGATGCCAACCTTGACAAGGTGCTGAGCGCATATAATAAACACCGTGATAAA
CCCATTCGTGAACAGGCTGAAAATATCATACATCTGTTCACGTTAACCAACTTGGGAGCT
CCTGCCGCTTTTAAATATTTCGATACCACAATTGACCGCAAACGTTATACGTCTACAAAA
GAGGTGCTCGATGCGACCCTGATCCACCAGTCTATTACAGGCCTGTATGAAACTCGTATC
GACCTGTCACAACTGGGCGGCGACTGA
SEQ ATGGACAAGAAATATTCAATCGGTTTAGCAATAGGAACTAACTCAGTAGGTTGGGCTGTA
ID ATTACAGACGAATACAAGGTACCGTCCAAAAAGTTTAAGGTGTTGGGGAACACAGATAGA
NO: CACTCTATAAAAAAAAATTTAATAGGCGCTTTACTTTTCGATTCAGGCGAAACTGCAGAA
169 GCGACACGTCTGAAGAGAACCGCTAGACGTAGATACACGAGGAGAAAGAACAGAATATGT
TACCTACAAGAAATTTTTTCTAATGAGATGGCTAAGGTGGATGATTCGTTTTTTCATAGA
CTCGAAGAATCTTTCTTAGTTGAAGAAGATAAAAAACACGAAAGGCATCCTATCTTTGGA
AACATAGTTGATGAGGTGGCTTACCATGAAAAATATCCCACTATATATCACCTTAGAAAA
AAGTTGGTTGATTCAACCGACAAAGCGGATCTAAGGTTAATTTACCTCGCGTTGGCTCAC
ATGATAAAATTTAGAGGACATTTCTTGATCGAAGGTGATTTAAATCCCGATAACTCTGAT
GTAGATAAACTGTTCATCCAGTTGGTTCAAACATATAATCAGTTGTTCGAAGAGAACCCC
ATTAACGCATCAGGTGTTGATGCTAAAGCAATCTTATCAGCAAGGTTGAGCAAGAGCAGA
CGTCTGGAAAACTTGATTGCCCAATTGCCAGGTGAAAAGAAGAACGGTCTTTTTGGAAAT
TTAATTGCACTTTCACTTGGGTTGACACCGAATTTTAAAAGCAATTTCGACCTCGCTGAG
GATGCTAAACTCCAGTTATCTAAGGATACATATGACGATGATTTGGATAATCTATTGGCC
CAGATAGGTGATCAGTATGCAGATTTGTTTTTGGCAGCTAAGAATTTATCAGATGCAATT
CTACTGAGCGATATTTTAAGGGTGAATACAGAAATAACTAAAGCACCTTTGTCTGCATCT
ATGATAAAAAGATACGATGAACACCATCAAGATCTCACACTATTAAAAGCTTTAGTTAGA
CAACAATTACCAGAAAAATATAAAGAAATCTTTTTCGATCAGTCCAAGAACGGATACGCC
GGCTATATAGATGGCGGTGCCTCCCAAGAAGAATTTTACAAATTTATCAAACCCATTTTG
GAAAAGATGGATGGTACTGAAGAATTATTGGTCAAATTAAACAGGGAAGATTTATTAAGA
AAACAAAGGACCTTTGATAATGGTTCTATTCCACACCAAATCCATCTAGGGGAATTACAT
GCGATTCTTAGAAGACAAGAAGATTTTTATCCATTCTTGAAAGATAACAGGGAAAAGATA
GAGAAAATCTTAACTTTTAGAATTCCCTACTACGTCGGGCCCTTAGCTAGGGGGAATTCT
AGATTCGCCTGGATGACACGCAAATCAGAAGAAACAATTACGCCTTGGAATTTTGAAGAA
GTTGTTGATAAAGGAGCCTCTGCTCAATCTTTTATTGAACGAATGACCAATTTTGATAAG
AATTTACCCAATGAAAAGGTCTTACCCAAACATTCACTCCTATACGAGTACTTTACTGTT
TACAATGAGTTGACAAAAGTGAAGTATGTTACCGAGGGTATGCGAAAACCTGCTTTCTTG
AGTGGTGAACAAAAGAAGGCCATTGTTGACTTGTTATTCAAAACTAACAGAAAGGTCACT
GTGAAGCAGCTTAAAGAAGATTATTTCAAAAAGATCGAATGTTTCGACTCGGTAGAAATT
AGTGGTGTGGAAGATAGATTTAATGCTTCTCTTGGAACATATCATGATCTACTAAAGATC
ATCAAAGATAAAGATTTCTTGGACAATGAAGAAAATGAAGATATTCTTGAAGACATCGTG
TTGACACTTACATTGTTTGAGGACAGAGAAATGATTGAAGAAAGGCTGAAGACCTACGCC
CATTTGTTTGATGATAAAGTCATGAAACAGTTAAAGAGGAGAAGGTATACCGGATGGGGT
AGGCTGTCTCGCAAATTGATTAATGGTATTCGTGATAAACAATCGGGTAAAACAATCCTA
GATTTCCTGAAGTCCGATGGTTTCGCCAACAGGAATTTTATGCAATTGATTCATGACGAT
TCTTTGACTTTTAAAGAGGATATTCAGAAAGCACAGGTCTCAGGACAGGGCGATTCACTC
CATGAACATATAGCTAACCTGGCTGGCTCCCCTGCTATTAAGAAAGGTATCTTGCAAACC
GTCAAAGTAGTAGACGAACTTGTTAAAGTTATGGGAAGACACAAACCTGAAAATATCGTT
ATTGAAATGGCTCGCGAAAACCAGACAACACAAAAGGGTCAAAAGAATTCGAGAGAGAGA
ATGAAGCGTATCGAAGAAGGTATTAAAGAACTTGGGTCCCAAATACTTAAAGAACATCCA
GTAGAAAACACTCAGCTTCAAAATGAAAAATTATACTTATATTATCTTCAGAATGGCCGC
GATATGTATGTTGACCAAGAGTTAGATATAAATAGGTTGTCTGATTACGACGTGGATCAT
ATTGTACCTCAATCTTTTCTAAAAGATGATTCAATTGATAATAAGGTATTAACGAGAAGT
GATAAAAATAGAGGTAAATCTGACAACGTGCCAAGCGAAGAGGTGGTGAAGAAAATGAAA
AATTATTGGCGTCAACTGTTGAACGCCAAGTTAATTACGCAGAGAAAGTTTGATAATCTA
ACAAAAGCTGAAAGAGGAGGCCTATCTGAGTTAGATAAGGCCGGTTTTATCAAACGTCAG
TTAGTTGAAACCAGGCAAATCACGAAGCACGTTGCCCAAATTCTAGATTCAAGGATGAAT
ACCAAATACGATGAAAACGATAAACTGATTCGGGAAGTCAAGGTTATAACTCTAAAAAGC
AAACTAGTTTCAGATTTTCGCAAAGATTTTCAATTTTACAAAGTTCGAGAAATCAATAAT
TATCATCATGCTCACGACGCGTACTTGAACGCGGTCGTTGGTACAGCTTTAATAAAGAAA
TATCCTAAACTGGAATCGGAATTTGTATATGGGGATTACAAAGTATACGACGTGAGAAAG
ATGATCGCTAAATCTGAACAAGAAATTGGGAAAGCAACTGCCAAATATTTTTTTTACAGC
AACATAATGAATTTTTTTAAAACGGAAATTACATTGGCAAATGGCGAAATTAGAAAGCGC
CCATTGATAGAGACCAATGGAGAGACTGGGGAAATCGTGTGGGATAAAGGACGTGATTTT
GCCACAGTGAGGAAAGTGTTAAGTATGCCACAAGTTAATATTGTAAAAAAGACCGAGGTC
CAAACGGGTGGATTTAGCAAAGAATCAATTTTACCTAAGAGAAATTCAGATAAATTAATT
GCCCGCAAAAAGGATTGGGATCCTAAAAAATATGGTGGTTTTGATTCCCCAACAGTTGCT
TACTCCGTCCTAGTTGTTGCTAAGGTTGAAAAAGGAAAGTCTAAGAAACTTAAATCCGTA
AAAGAGTTACTGGGAATTACAATAATGGAAAGATCCTCTTTCGAAAAGAACCCTATTGAC
TTCTTGGAGGCGAAAGGTTATAAAGAAGTCAAAAAAGATTTGATCATAAAACTACCAAAG
TATTCTCTATTTGAATTGGAAAACGGCAGAAAAAGGATGTTGGCAAGCGCTGGTGAACTA
CAAAAGGGTAACGAATTGGCATTGCCGAGTAAATACGTGAATTTTCTATATTTGGCATCA
CATTACGAAAAGTTAAAGGGATCACCCGAGGATAACGAGCAGAAACAACTGTTTGTTGAA
CAACACAAACATTATCTTGATGAAATTATAGAACAAATTAGTGAGTTCAGTAAGAGAGTT
ATTTTAGCCGATGCAAATTTAGACAAAGTTTTATCTGCTTATAACAAACATAGAGATAAG
CCTATAAGGGAACAAGCCGAAAATATTATTCATTTGTTTACGTTAACAAATTTAGGGGCA
CCAGCAGCATTCAAGTACTTCGATACGACTATCGATCGTAAGCGTTACACATCTACCAAA
GAAGTTCTTGATGCAACTTTGATTCATCAATCTATAACAGGCTTATATGAAACTAGAATC
GATCTGTCACAACTTGGTGGTGACTAA
SEQ ATGGACAAGAAGTACTCAATTGGGCTTGCTATCGGCACTAACAGCGTTGGCTGGGCGGTC
ID ATCACAGACGAATATAAGGTCCCATCAAAGAAATTCAAAGTCCTTGGCAATACGGACCGA
NO: CATTCAATCAAGAAGAACCTGATTGGAGCTCTGCTGTTTGATTCCGGTGAAACCGCCGAG
170 GCAACACGATTGAAACGTACCGCTCGTAGGAGGTATACGCGGCGGAAAAATAGGATCTGC
TATCTGCAGGAAATATTTAGCAACGAAATGGCCAAGGTAGACGACAGCTTCTTCCACCGG
CTCGAGGAATCTTTCCTCGTGGAAGAAGACAAAAAGCACGAGCGCCACCCCATTTTCGGC
AATATCGTGGACGAGGTAGCTTACCATGAAAAGTATCCAACTATTTACCACTTACGTAAG
AAGTTAGTGGACAGCACCGATAAAGCCGACCTTCGCCTGATTTACCTAGCACTTGCACAC
ATGATTAAGTTCCGAGGCCACTTCTTGATAGAGGGAGACCTGAATCCTGACAATTCCGAT
GTGGATAAATTGTTCATCCAGCTGGTACAGACATACAATCAGTTGTTTGAGGAAAATCCG
ATTAATGCCAGTGGCGTGGACGCCAAGGCTATCCTGTCTGCTCGGCTTAGTAAGAGTAGA
CGCCTGGAAAATCTAATCGCACAGCTGCCCGGCGAAAAGAAAAATGGACTGTTCGGTAAT
TTGATCGCCCTGAGCCTGGGCCTCACCCCTAACTTTAAGTCTAACTTCGACCTGGCCGAA
GATGCTAAGCTCCAGCTGTCCAAAGATACTTACGATGACGATCTCGATAATCTACTGGCT
CAGATCGGGGACCAGTACGCTGACCTGTTTCTAGCTGCCAAGAACCTCAGTGACGCCATT
CTCCTGTCCGATATTCTGAGGGTTAACACTGAAATTACAAAGGCCCCGCTGAGCGCGAGC
ATGATCAAAAGGTACGACGAGCATCACCAGGACCTCACGCTGCTGAAGGCCTTAGTCAGA
CAGCAACTGCCCGAAAAGTACAAAGAAATCTTTTTCGACCAATCCAAGAACGGGTACGCC
GGCTACATTGATGGCGGGGCTTCACAAGAGGAGTTTTACAAGTTTATCAAGCCCATCCTG
GAGAAAATGGACGGCACTGAAGAACTGCTTGTGAAACTCAATAGGGAAGACTTACTGAGG
AAACAGCGCACATTCGATAATGGCTCCATACCCCACCAAATCCATCTGGGAGAGTTGCAT
GCCATCTTGCGAAGGCAGGAGGACTTCTACCCCTTTCTTAAGGACAACAGGGAGAAAATC
GAGAAAATTCTGACTTTCCGTATCCCCTACTACGTGGGCCCACTTGCTCGCGGAAACTCA
CGATTCGCATGGATGACCAGAAAGTCCGAGGAAACAATTACACCCTGGAATTTTGAGGAG
GTAGTAGACAAGGGAGCCAGCGCTCAATCTTTCATTGAGAGGATGACGAATTTCGACAAG
AACCTTCCAAACGAGAAAGTGCTTCCTAAGCACAGCCTGCTGTATGAGTATTTCACGGTG
TACAACGAACTTACGAAGGTCAAGTATGTGACAGAGGGTATGCGGAAACCTGCTTTTCTG
TCTGGTGAACAGAAGAAAGCTATCGTCGATCTCCTGTTTAAAACCAACCGAAAGGTGACG
GTGAAACAGTTGAAGGAGGATTACTTCAAGAAGATCGAGTGTTTTGATTCTGTTGAAATT
TCTGGGGTCGAGGATAGATTCAACGCCAGCCTGGGCACCTACCATGATTTGCTGAAGATT
ATCAAGGATAAGGATTTTCTGGATAATGAGGAGAATGAAGACATTTTGGAGGATATAGTG
CTGACCCTCACCCTGTTCGAGGACCGGGAGATGATCGAGGAGAGACTGAAAACATACGCT
CACCTGTTTGACGACAAGGTCATGAAGCAGCTTAAGAGACGCCGTTACACAGGCTGGGGA
AGATTATCCCGCAAATTAATCAACGGGATACGCGATAAACAAAGTGGCAAGACCATACTC
GACTTCCTAAAGAGCGATGGATTCGCAAATCGCAATTTCATGCAGTTGATCCACGACGAT
AGCCTGACCTTCAAAGAGGACATTCAGAAAGCGCAGGTGAGTGGTCAAGGGGATTCCCTG
CACGAACACATTGCTAACTTGGCTGGATCACCAGCCATTAAGAAAGGCATACTGCAGACC
GTTAAAGTGGTAGATGAGCTTGTGAAAGTCATGGGAAGACATAAGCCAGAGAACATAGTG
ATCGAAATGGCCAGGGAAAATCAGACCACGCAAAAGGGGCAGAAGAACTCAAGAGAGCGT
ATGAAGAGGATCGAGGAGGGCATCAAGGAGCTGGGTAGCCAGATCCTTAAAGAGCACCCA
GTTGAGAATACCCAGCTGCAGAATGAGAAACTTTATCTCTATTATCTCCAGAACGGAAGG
GATATGTATGTCGACCAGGAACTGGACATCAATCGGCTGAGTGATTATGACGTCGACCAC
ATTGTGCCTCAAAGCTTTCTGAAGGATGATTCCATCGACAATAAAGTTCTGACCCGGTCT
GATAAAAATAGAGGCAAATCCGACAACGTACCTAGCGAAGAAGTCGTCAAAAAAATGAAG
AACTATTGGAGGCAGTTGCTGAATGCCAAGCTGATTACACAACGCAAGTTTGACAATCTC
ACCAAGGCAGAAAGGGGGGGCCTGTCAGAACTCGACAAAGCAGGTTTCATTAAAAGGCAG
CTAGTTGAAACTAGGCAGATTACTAAGCACGTGGCCCAGATCCTCGACTCACGGATGAAT
ACAAAGTATGATGAGAATGATAAGCTAATCCGGGAGGTGAAGGTGATTACTCTGAAATCT
AAGCTGGTGTCAGATTTCAGAAAAGACTTCCAGTTCTACAAAGTCAGAGAGATCAACAAT
TATCACCATGCCCACGATGCATATCTTAATGCAGTAGTGGGGACAGCTCTGATCAAAAAA
TATCCTAAACTGGAGTCTGAATTCGTTTATGGTGACTATAAAGTCTATGACGTCAGAAAA
ATGATCGCAAAGAGCGAGCAGGAGATAGGGAAGGCCACAGCAAAGTACTTCTTTTACAGT
AATATCATGAACTTTTTCAAAACTGAGATTACATTGGCTAACGGCGAGATCCGCAAGCGG
CCACTGATAGAGACTAACGGAGAGACAGGGGAGATTGTTTGGGATAAGGGCCGTGACTTC
GCCACCGTTAGGAAAGTGCTGTCCATGCCCCAGGTGAACATTGTGAAGAAGACAGAAGTG
CAGACGGGTGGGTTCTCAAAAGAGTCTATTCTGCCTAAGCGGAATAGTGACAAACTGATC
GCACGTAAAAAGGACTGGGATCCAAAAAAGTACGGCGGATTCGACAGTCCTACCGTTGCA
TATTCCGTGCTTGTGGTCGCTAAGGTGGAGAAGGGAAAAAGCAAGAAACTGAAGTCAGTC
AAAGAACTACTGGGCATAACGATCATGGAGCGCTCCAGTTTCGAAAAAAACCCAATCGAT
TTTCTTGAAGCCAAGGGATACAAGGAGGTAAAGAAAGACCTTATCATTAAGCTGCCTAAG
TACAGTCTGTTCGAACTGGAGAATGGGAGGAAGCGCATGCTGGCATCAGCTGGAGAACTC
CAAAAAGGGAACGAGTTGGCCCTCCCCTCAAAGTATGTCAATTTTCTCTACCTGGCTTCT
CACTACGAGAAGTTAAAGGGGTCTCCAGAGGATAATGAGCAGAAACAGCTGTTTGTGGAA
CAGCACAAGCACTATTTGGACGAAATCATCGAACAAATTTCCGAGTTCAGTAAGAGGGTG
ATTCTGGCCGACGCAAACCTTGACAAAGTTCTGTCCGCATACAATAAGCACAGAGACAAA
CCAATCCGCGAGCAAGCCGAGAATATAATTCACCTTTTCACTCTGACTAATCTGGGGGCC
CCCGCAGCATTTAAATATTTCGATACAACAATCGACCGGAAGCGGTATACATCTACTAAG
GAAGTCCTCGATGCGACACTGATCCACCAGTCAATTACAGGTTTATATGAAACAAGAATC
GACCTGTCCCAGCTGGGCGGCGACTAG
SEQ AAAATTCcatGCAAAATGCTCCGGTTTCATGTCATCAAAATGATGACGTAATTAAGCATT
ID GATAATTGAGATCCCTCTCCCTGACAGGATGATTACATAAATAATAGTGACAAAAATAAA
NO: TTATTTATTTATCCAGAAAATGAATTGGAAAATCAGGAGAGCGTTTTCAATCCTACCTCT
171 GGCGCAGTTGATATGTcaaaCAGGTtgccgtcactgcgtcttttactggctcttctcgct
aaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccat
gacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattattt
gcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctac
ctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagcac
cgcctatctcgtgtgagataggcggagatacgaactttaagAAGGAGatataccATGGAA
CAGGAATATTATCTGGGCTTGGACATGGGCACCGGTTCCGTCGGCTGGGCTGTTACTGAC
AGTGAATATCACGTTCTAAGAAAGCATGGTAAGGCATTGTGGGGTGTAAGACTTTTCGAA
TCTGCTTCCACTGCTGAAGAGCGTAGAATGTTTAGAACGAGTCGACGTAGGCTAGACAGG
CGCAATTGGAGAATCGAAATTTTACAAGAAATTTTTGCGGAAGAGATATCTAAGAAAGAC
CCAGGCTTTTTCCTGAGAATGAAGGAATCTAAGTATTACCCTGAGGATAAAAGAGATATA
AATGGTAACTGTCCCGAATTGCCTTACGCATTATTTGTGGACGATGATTTTACCGATAAG
GATTACCATAAAAAGTTCCCAACTATCTACCATTTACGCAAAATGTTAATGAATACAGAG
GAAACCCCAGACATAAGACTAGTTTATCTGGCAATACACCATATGATGAAACATAGAGGC
CATTTCTTACTTTCCGGGGATATCAACGAAATCAAAGAGTTTGGTACCACATTTAGTAAG
TTACTGGAAAACATAAAGAATGAAGAATTGGATTGGAACTTAGAACTCGGAAAAGAAGAA
TACGCGGTTGTCGAATCTATCCTGAAGGATAATATGCTGAATAGGTCGACCAAAAAAACT
AGGCTGATCAAAGCACTGAAAGCCAAATCTATCTGCGAAAAAGCTGTTTTAAATTTACTT
GCTGGTGGCACTGTTAAGTTATCAGACATTTTTGGTTTGGAAGAATTGAACGAAACCGAG
CGTCCAAAAATTAGTTTCGCTGATAATGGCTACGATGATTACATTGGTGAGGTGGAAAAC
GAGTTGGGCGAACAATTTTATATTATAGAGACAGCTAAGGCAGTCTATGACTGGGCTGTT
TTAGTAGAAATCCTTGGTAAATACACATCTATCTCCGAAGCGAAAGTTGCTACTTACGAA
AAGCACAAGTCCGATCTCCAGTTTTTGAAGAAAATTGTCAGGAAATATCTGACTAAGGAA
GAATATAAAGATATTTTCGTTAGTACCTCTGACAAACTGAAAAATTACTCCGCTTACATC
GGGATGACCAAGATTAATGGCAAAAAAGTTGATCTGCAAAGCAAAAGGTGTTCGAAGGAA
GAATTTTATGATTTCATTAAAAAGAATGTCTTAAAAAAATTAGAAGGTCAGCCAGAATAC
GAATATTTGAAAGAAGAACTGGAAAGAGAGACATTCTTACCAAAACAAGTCAACAGAGAT
AATGGGGTAATTCCATATCAAATTCACCTCTACGAATTAAAAAAAATTTTAGGCAATTTA
CGCGATAAAATTGACCTTATCAAAGAAAATGAGGATAAGCTGGTTCAACTCTTTGAATTC
AGAATACCCTATTATGTGGGCCCACTGAACAAGATTGATGACGGCAAAGAAGGTAAATTC
ACATGGGCCGTCCGCAAATCCAATGAAAAAATTTACCCATGGAACTTTGAAAATGTAGTA
GATATTGAAGCGTCTGCGGAGAAATTTATTCGAAGAATGACTAATAAATGCACTTACTTG
ATGGGAGAGGATGTTCTGCCTAAAGACAGCTTATTATACAGCAAGTACATGGTTCTAAAC
GAACTTAACAACGTTAAGTTGGACGGTGAGAAATTAAGTGTAGAATTGAAACAAAGATTG
TATACTGACGTCTTCTGCAAGTACAGAAAAGTGACAGTTAAAAAAATTAAGAATTACTTG
AAGTGCGAAGGTATAATTTCTGGAAACGTAGAGATTACTGGTATTGATGGTGATTTCAAA
GCATCCCTAACAGCTTACCACGATTTCAAGGAAATCCTGACAGGAACTGAACTCGCAAAA
AAAGATAAAGAAAACATTATTACTAATATTGTTCTTTTCGGTGATGACAAGAAATTGTTG
AAGAAAAGACTGAATAGACTTTACCCCCAGATTACTCCCAATCAACTTAAGAAAATTTGT
GCTTTGTCTTACACAGGATGGGGTCGTTTTTCAAAAAAGTTCTTAGAAGAGATTACCGCA
CCTGATCCAGAAACAGGCGAAGTATGGAATATAATTACCGCCTTATGGGAATCGAACAAT
AATCTTATGCAACTTCTGAGCAATGAATATCGTTTCATGGAAGAAGTTGAGACTTACAAC
ATGGGCAAACAGACGAAGACTTTATCCTATGAAACTGTGGAAAATATGTATGTATCACCT
TCTGTCAAGAGACAAATTTGGCAAACCTTAAAAATTGTCAAAGAATTAGAAAAGGTAATG
AAGGAGTCTCCTAAACGTGTGTTTATTGAAATGGCTAGAGAAAAACAAGAGTCAAAAAGA
ACCGAGTCAAGAAAGAAGCAGTTAATCGATTTATATAAGGCTTGTAAAAACGAAGAGAAA
GATTGGGTTAAAGAATTGGGGGACCAAGAGGAACAAAAACTACGGTCGGATAAGTTGTAT
TTATACTATACGCAAAAGGGACGATGTATGTATTCCGGCGAGGTAATAGAATTGAAGGAT
TTATGGGACAATACAAAATATGACATAGACCATATATATCCCCAATCAAAAACGATGGAC
GATAGCTTGAACAATAGAGTACTCGTGAAAAAAAAATATAATGCGACCAAATCTGATAAG
TATCCTCTGAATGAAAATATCAGACATGAAAGAAAGGGGTTCTGGAAGTCCTTGTTAGAT
GGTGGGTTTATAAGCAAAGAAAAGTACGAGCGTCTAATAAGAAACACGGAGTTATCGCCA
GAAGAACTCGCTGGTTTTATTGAGAGGCAAATCGTGGAAACGAGACAATCTACCAAAGCC
GTTGCTGAGATCCTAAAGCAAGTTTTCCCAGAGTCGGAGATTGTCTATGTCAAAGCTGGC
ACAGTGAGCAGGTTTAGGAAAGACTTCGAACTATTAAAGGTAAGAGAAGTGAACGATTTA
CATCACGCAAAGGACGCTTACCTAAATATCGTTGTAGGTAACTCATATTATGTTAAATTT
ACCAAGAACGCCTCTTGGTTTATAAAGGAGAACCCAGGTAGAACATATAACCTGAAAAAG
ATGTTCACCTCTGGTTGGAATATTGAGAGAAACGGAGAAGTCGCATGGGAAGTTGGTAAG
AAAGGGACTATAGTGACAGTAAAGCAAATTATGAACAAAAATAATATCCTCGTTACAAGG
CAGGTTCATGAAGCAAAGGGCGGCCTTTTTGACCAACAAATTATGAAGAAAGGGAAAGGT
CAAATTGCAATAAAAGAAACCGATGAGAGACTAGCGTCAATAGAAAAGTATGGTGGCTAT
AATAAAGCTGCGGGTGCATACTTTATGCTTGTTGAATCAAAAGACAAGAAAGGTAAGACT
ATTAGAACTATAGAATTTATACCCCTGTACCTTAAAAACAAAATTGAATCGGATGAGTCA
ATCGCGTTAAATTTTCTAGAGAAAGGAAGGGGTTTAAAAGAACCAAAGATCCTGTTAAAA
AAGATTAAGATTGACACCTTGTTCGATGTAGATGGATTTAAAATGTGGTTATCTGGCAGA
ACAGGCGATAGACTTTTGTTTAAGTGCGCTAATCAATTAATTTTGGATGAGAAAATCATT
GTCACAATGAAAAAAATAGTTAAGTTTATTCAGAGAAGACAAGAAAACAGGGAGTTGAAA
TTATCTGATAAAGATGGTATCGACAATGAAGTTTTAATGGAAATCTACAATACATTCGTT
GATAAACTTGAAAATACCGTATATCGAATCAGGTTAAGTGAACAAGCCAAAACATTAATT
GATAAACAAAAAGAATTTGAAAGGCTATCACTGGAAGACAAATCCTCCACCCTATTTGAA
ATTTTGCATATATTCCAGTGCCAATCTTCAGCAGCTAATTTAAAAATGATTGGCGGACCT
GGGAAAGCCGGCATCCTAGTGATGAACAATAATATCTCCAAGTGTAACAAAATATCAATT
ATTAACCAATCTCCGACAGGTATTTTTGAAAATGAAATAGACTTGCTTAAGATATAAGAA
ATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATTTATTATATCGCGTTGATT
ATTGATGCTGTTTTTAGTTTTAACGGCAATTAATATATGTGTTATTAATTGAATGAATTT
TATCATTCATAATAAGTATGTGTAGGATCAAGCTCAGGTTAAATATTCACTCAGGAAGTT
ATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATAACAAGTGTTAAGGGATGTTA
TTTCC
SEQ AATTCAAAGGATAATCAAAC
ID
NO:
172
SEQ AATCTCTACTCTTTGTAGAT
ID
NO:
173
SEQ AATTTCTACTGTTGTAGAT
ID
NO:
174
SEQ AATTTCTACTAGTGTAGAT
ID
NO:
175
SEQ AATTTCTACTATTGT
ID
NO:
176
SEQ AATTTCTACTGTTGTAGA
ID
NO:
177
SEQ AATTTCTACTATTGTA
ID
NO:
178
SEQ AATTTCTACTTTTGTAGAT
ID
NO:
179
SEQ AATTTCTACTGTTGTAGAT
ID
NO:
180
SEQ AATTTCTACTCTTGTAGAT
ID
NO:
181
