IDENTIFICATION AND ISOLATION OF HUMAN CORNEAL ENDOTHELIAL CELLS (HCECS)

The present invention provides methods for the identification, isolation and/or enrichment of human corneal endothelial cells (HCECs). In some embodiments, the method comprises a positive selection process in which a cell population containing human corneal cells is contacted with a positive affinity reagent that selectively binds to HCECs relative to cells other than HCECs (e.g., corneal keratocytes, etc.) in the population and/or a negative selection process in which a cell population containing HCECs is contacted with a negative affinity reagent that selectively binds to cells other than HCECs in the population relative to HCECs. The present invention also provides reagents and kits for the identification, isolation and/or enrichment of HCECs as well as compositions that are enriched in HCECs.

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Description
CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a continuations of U.S. patent application Ser. No. 14/888,875, filed Nov. 3, 2015 (published as US20160102290), which is the U.S. National Stage of International Patent Application No. PCT/US2014/036616, filed May 2, 2014, which claims the benefit of U.S. Patent Application No. 61/819,146, entitled “METHOD FOR IDENTIFYING AND ISOLATING HUMAN CORNEAL ENDOTHELIAL CELLS (HCECS),” filed May 3, 2013, the contents of each of which are hereby incorporated by reference in their entireties.

INCORPORATION-BY-REFERENCE OF MATERIAL ELECTRONICALLY FILED

Incorporated by reference in its entirety herein is a computer-readable nucleotide/amino acid sequence listing submitted concurrently herewith and identified as follows: One 821,867 byte ASCII (text) file named “Seq_List” created on Aug. 13, 2018.

BACKGROUND OF THE INVENTION

When the innermost layer of the cornea, the endothelium, is damaged, for example from trauma (e.g., from cataract surgery), disease or dystrophy, the cornea swells with fluid (edema) and loses its optical clarity. Patients consequently suffer from vision loss and pain, and their only option to treat advanced disease is with corneal transplant surgery (also known as penetrating keratoplasty, PK) or Descemet's stripping endothelial keratoplasty (DSAEK), both technically difficult procedures that are very invasive to the patient and have significant limitations, such as the number of donor corneas available.

Recent studies have proposed the use of human corneal endothelial cells (HCECs) obtained from cadaveric donors to replace the damaged cells. See, e.g., Joyce and Zhu, Cornea. 2004 November; 23(8 Suppl):S8-S19; Engelmann, et al., Exper. Eye Res., vol. 78, no. 3, pp. 573-578, 2004. A potential advantage to such an approach could be the expansion of HCECs ex vivo before implantation into patients, thereby overcoming the limited tissue availability. HCECs can be expanded in defined tissue culture media for at least 5 passages, greatly expanding the number of cells derived from a single donor.

One of the main problems with such a technique is that the lack of defined surface markers specific for HCECs makes it difficult to confirm the identity of HCECs after several passages, or to select HCECs away from contaminating cells, or to identify the subset of HCECs that are likely to have the highest clinical efficacy from among the full population of HCECs, as current identification criteria are limited to cell morphology and the expression of functional genes, such as ATP1A1 (see, e.g., Kaye and Tice, Invest Ophthalmol 1966; 522-32; Leuenberger and Novikoff, J Cell Biol. 1974; 60721-731; McCartney et al., Curr Eye Res, 1987; 61479-1486) or the tight junction marker zonula occludens-1(ZO-1) (see, e.g., Petroll et al., Curr Eye Res. 1999 January; 18(1):10-9), neither of which are specific to HCECs. It is also difficult to isolate HCECs from contaminant fibroblasts in culture, from neighboring cells in whole corneas, or from residual corneas from DSAEK.

In this regard, the current isolation method for obtaining HCECs from intact corneas comprises a peel-off step, where the endothelium and its basement membrane (Descemet's membrane) are peeled off the stroma and collected. See, e.g., Ko-Hua Chen et al., “Transplantation of Adult Human Corneal Endothelium Ex Vivo: A Morphologic Study,” Cornea 20(7): 731-737, 2001. The tissue collected thus contains HCECs, but it may also contain corneal keratocytes (specialized fibroblasts residing the stroma). Corneal keratocytes (also referred to herein simply as “keratocytes”) are undesirable contaminants in the HCECs culture, as they grow faster than the latter cells and they can take over the culture dish, thus making the final product essentially useless. In addition to residual stromal tissue, keratocytes may also arise from human endothelial cells which transform spontaneously into other types of cells such as keratocytes (see, e.g., G S. L. Peh et al., “Optimization of Human Corneal Endothelial Cells for Culture: The Removal of Corneal Stromal Fibroblast Contamination Using Magnetic Cell Separation,” International Journal of Biomaterials, Volume 2012 (2012), Article ID 601302, 8 pages.)

SUMMARY OF THE INVENTION

Some aspects of the invention are directed to methods for the identification, enrichment and/or isolation of human corneal endothelial cells (HCECs).

In some embodiments, the method comprises a positive selection process in which a cell population containing human corneal cells is contacted with a positive affinity reagent that selectively binds to HCECs relative to cells other than HCECs. The cells to which the positive affinity reagent is bound are then selected, with the result being that selected cells are enriched with HCECs. In some embodiments, two or more differing positive affinity reagents which bind to HCECs but which do not bind to cells other than HCECs are employed.

As defined herein “cells other than human corneal endothelial cells” (or “cells other than HCECs”) include corneal keratocytes as well as HCECs of lower utility (e.g., HCECs that have undergone fibroblastic or mesenchymal transformation, etc.).

In other embodiments, the method comprises a negative selection process in which a cell population containing human corneal cells is contacted with a negative affinity reagent that selectively binds to cells other than HCECs (e.g., corneal keratocytes, etc.) relative to HCECs. The cells to which the negative affinity reagent is bound are then removed, with the result being that the cells that are not removed are enriched with HCECs. In some embodiments, two or more differing negative affinity reagents which bind to cells other than HCECs (e.g., corneal keratocytes, etc.) but which do not bind to HCECs are employed.

In some embodiments, the method comprises both (a) positive selection using one or more affinity reagents agents and (b) negative selection using one or more negative affinity reagents.

Other aspects of the invention are directed to affinity reagents and kits useful for the identification, enrichment and/or isolation of HCECs.

Still other aspects of the invention are direct to isolated and/or enriched cell populations that contain HCECs. In some embodiments, such enriched cell populations may contain affinity reagents for the purification of HCECs.

Further aspects of the invention a set forth in the following paragraphs:

    • Aspect 1. A method of forming a composition enriched with human corneal endothelial cells comprising: (a) contacting a cell population containing human corneal cells with a first positive affinity reagent that selectively binds to human corneal endothelial cells relative to cells other than human corneal endothelial cells and (b) selecting cells to which the first positive affinity reagent is bound.
    • Aspect 2. The method of aspect 1, wherein the first positive affinity reagent selectively binds to human corneal endothelial cells relative to corneal keratocytes, human corneal endothelial cells of lower utility, or both.
    • Aspect 3. The method of aspect 1, wherein the first positive affinity reagent selectively binds to a corneal protein selected from protein products of genes X1 through X26 of Table 2, and may comprise, for example, an antibody or aptamer that binds to a corneal protein selected from protein products of genes X1 through X26 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO (1) through SEQ ID NO (58) of Table 2.
    • Aspect 4. The method of aspect 1, wherein the first positive affinity reagent comprises an antibody or aptamer that binds to a protein product of gene X5 of Table 2, an antibody or aptamer that binds to a protein product of gene X15 of Table 2, or an antibody or aptamer that binds to a protein product of X25 of Table 2.
    • Aspect 5. The method of aspect 1, wherein the first positive affinity reagent comprises (a) an antibody or aptamer that binds to SEQ ID NO (8), (b) an antibody or aptamer that binds to one or more of SEQ ID NO (27), SEQ ID NO (28), SEQ ID NO (29), SEQ ID NO (30) or SEQ ID NO (31), or (c) an antibody or aptamer that binds to one or more of SEQ ID NO (53), SEQ ID NO (54), SEQ ID NO (55), SEQ ID NO (56) or SEQ ID NO (57).
    • Aspect 6. The method of any of aspects 1-5, wherein the first positive affinity reagent comprises an antibody or aptamer that is coupled to a solid matrix.
    • Aspect 7. The method of any of aspects 1-6, wherein the first positive affinity reagent comprises an antibody or aptamer that is coupled to a label.
    • Aspect 8. The method of aspect 7, wherein the label is selected from a magnetic label, a hapten (e.g., biotin) and a fluorescent label.
    • Aspect 9. The method of any of aspects 1-8, further comprising (a) contacting said cell population containing human corneal cells with a second positive affinity reagent that selectively binds to human corneal endothelial cells relative to cells other than human corneal endothelial cells and (b) selecting cells to which the second positive affinity reagent is bound, wherein the second positive affinity reagent differs from the first positive affinity reagent.
    • Aspect 10. The method of aspect 9, wherein the second positive affinity reagent selectively binds to a corneal protein selected from protein products of genes X1 through X26 of Table 2, and may comprise, for example, an antibody or aptamer that binds to a corneal protein selected from protein products of genes X1 through X26 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO (1) through SEQ ID NO (58) of Table 2.
    • Aspect 11. The method of aspect 9, wherein the second positive affinity reagent comprises an antibody or aptamer that binds to a protein product of gene X5 of Table 2, an antibody or aptamer that binds to a protein product of gene X15 of Table 2, or an antibody or aptamer that binds to a protein product of X25 of Table 2.
    • Aspect 12. The method of aspect 9, wherein the second positive affinity reagent comprises (a) an antibody or aptamer that binds to SEQ ID NO (8), (b) an antibody or aptamer that binds to one or more of SEQ ID NO (27), SEQ ID NO (28), SEQ ID NO (29), SEQ ID NO (30) or SEQ ID NO (31), or (c) an antibody or aptamer that binds to one or more of SEQ ID NO (53), SEQ ID NO (54), SEQ ID NO (55), SEQ ID NO (56) or SEQ ID NO (57).
    • Aspect 13. The method of any of aspects 9-12, wherein the second positive affinity reagent comprises an antibody or aptamer that is coupled to a solid matrix or a label.
    • Aspect 14. The method of any of aspects 1-13, further comprising (a) contacting said cell population containing human corneal cells with a first negative affinity reagent that selectively binds to cells other than human corneal endothelial cells relative to human corneal endothelial cells and (b) removing the cells to which the first negative affinity reagent is bound.
    • Aspect 15. The method of aspect 14, wherein the first negative affinity reagent selectively binds to corneal keratocytes, human corneal endothelial cells of lower utility, or both, relative to human corneal endothelial cells.
    • Aspect 16. The method of aspect 14, wherein the first negative affinity reagent selectively binds to a corneal protein selected from protein products of genes Y1 through Y23 of Table 2, and may comprise, for example, an antibody or aptamer that binds to a corneal protein selected from protein products of genes Y1 through Y23 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO (59) through SEQ ID NO (96) of Table 2, or wherein the first negative affinity reagent selectively binds to a corneal protein selected from protein products of genes Z1 through Z8 of Table 2, and may comprise, for example, an antibody or aptamer that binds to a corneal protein selected from protein products of genes Z1 through Z8 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO (97) through SEQ ID NO (109) of Table 2.
    • Aspect 17. The method of aspect 14, wherein the first negative affinity reagent comprises an antibody or aptamer that binds to a protein product of gene Y6 of Table 2.
    • Aspect 18. The method of aspect 14, wherein the first negative affinity reagent comprises an antibody or aptamer that binds to one or more of SEQ ID NO (66), SEQ ID NO (67) or SEQ ID NO (68).
    • Aspect 19. The method of any of aspects 14-18, wherein the first negative affinity reagent comprises an antibody or aptamer that is coupled to a solid matrix or a label.
    • Aspect 20. A composition enriched with human corneal endothelial cells that is made by the method of any of aspects 1-19.
    • Aspect 21. A kit comprising (a) a positive affinity reagent that selectively binds to human corneal endothelial cells relative to cells other than human corneal endothelial cells and (b) a negative affinity reagent that selectively binds to cells other than human corneal endothelial cells relative to human corneal endothelial cells.
    • Aspect 22. The kit of aspect 21, wherein the positive affinity reagent selectively binds to a corneal protein selected from protein products of genes X1 through X26 of Table 2, and may comprise, for example, an antibody or aptamer that binds to a corneal protein selected from protein products of genes X1 through X26 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO (1) through SEQ ID NO (58) of Table 2.
    • Aspect 23. The kit of aspect 21, wherein the first positive affinity reagent comprises an antibody or aptamer that binds to a protein product of gene X5 of Table 2, an antibody or aptamer that binds to a protein product of gene X15 of Table 2, or an antibody or aptamer that binds to a protein product of X25 of Table 2.
    • Aspect 24. The kit of aspect 21, wherein the first positive affinity reagent comprises (a) an antibody or aptamer that binds to SEQ ID NO (8), (b) an antibody or aptamer that binds to one or more of SEQ ID NO (27), SEQ ID NO (28), SEQ ID NO (29), SEQ ID NO (30) or SEQ ID NO (31), or (c) an antibody or aptamer that binds to one or more of SEQ ID NO (53), SEQ ID NO (54), SEQ ID NO (55), SEQ ID NO (56) or SEQ ID NO (57).
    • Aspect 25. The kit of any of aspects 21-24, wherein the positive affinity reagent comprises an antibody or aptamer that is coupled to a solid matrix or a label.
    • Aspect 26. The kit of any of aspects 21-25, wherein the negative affinity reagent selectively binds to a corneal protein selected from protein products of genes Y1 through Y23 of Table 2, and may comprise, for example, an antibody or aptamer that binds to a corneal protein selected from protein products of genes Y1 through Y23 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO (59) through SEQ ID NO (96) of Table 2, or wherein the negative affinity reagent selectively binds to a corneal protein selected from protein products of genes Z1 through Z8 of Table 2, and may comprise, for example, an antibody or aptamer that binds to a corneal protein selected from protein products of genes Z1 through Z8 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO (97) through SEQ ID NO (109) of Table 2.
    • Aspect 27. The kit of any of aspects 21-25, wherein the negative affinity reagent comprises an antibody or aptamer that binds to a protein product of gene Y6 of Table 2.
    • Aspect 28. The kit of any of aspects 21-25, wherein the first negative affinity reagent comprises an antibody or aptamer that binds to one or more of SEQ ID NO (66), SEQ ID NO (67) or SEQ ID NO (68).
    • Aspect 29. The kit of any of aspects 21-28, wherein the negative affinity reagent comprises an antibody or aptamer that is coupled to a solid matrix or a label.
    • Aspect 30. An affinity reagent selected from (a) a solid matrix or a label coupled to an antibody or aptamer that binds to a corneal protein selected from protein products of genes X1 through X26 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO (1) through SEQ ID NO (58) of Table 2, and (b) a solid matrix or a label coupled to an antibody or aptamer that binds to a corneal protein selected from protein products of genes Y1 through Y23 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO (59) through SEQ ID NO (96) of Table 2, and protein products of genes Z1 through Z8 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO (97) through SEQ ID NO (109) of Table 2.
    • Aspect 31. A composition enriched with human corneal endothelial cells comprising: (a) human corneal cells and (b) a first positive affinity reagent that selectively binds to human corneal endothelial cells relative to cells other than human corneal endothelial cells.
    • Aspect 32. The composition of aspect 31, comprising human corneal endothelial cells to which the first positive affinity reagent is bound.
    • Aspect 33. The composition of any of aspects 31-32, wherein the first positive affinity reagent selectively binds to human corneal endothelial cells relative to corneal keratocytes, human corneal endothelial cells of lower utility, or both.
    • Aspect 34. The composition of any of aspects 31-33, wherein the first positive affinity reagent selectively binds to a corneal protein selected from protein products of genes X1 through X26 of Table 2, and may comprise, for example, an antibody or aptamer that binds to a corneal protein selected from protein products of genes X1 through X26 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO (1) through SEQ ID NO (58) of Table 2.
    • Aspect 35. The composition of any of aspects 31-33, wherein the first positive affinity reagent comprises an antibody or aptamer that binds to a protein product of gene X5 of Table 2, an antibody or aptamer that binds to a protein product of gene X15 of Table 2, or an antibody or aptamer that binds to a protein product of X25 of Table 2.
    • Aspect 36. The composition of any of aspects 31-33, wherein the first positive affinity reagent comprises (a) an antibody or aptamer that binds to SEQ ID NO (8), (b) an antibody or aptamer that binds to one or more of SEQ ID NO (27), SEQ ID NO (28), SEQ ID NO (29), SEQ ID NO (30) or SEQ ID NO (31), or (c) an antibody or aptamer that binds to one or more of SEQ ID NO (53), SEQ ID NO (54), SEQ ID NO (55), SEQ ID NO (56) or SEQ ID NO (57).
    • Aspect 37. The composition of any of aspects 31-36, wherein the first positive affinity reagent comprises an antibody or aptamer that is coupled to a label.
    • Aspect 38. The composition of aspect 37, wherein the label is selected from a magnetic label, a hapten (e.g., biotin) and a fluorescent label.
    • Aspect 39. The composition of any of aspects 31-38, further comprising a second positive affinity reagent that selectively binds to human corneal endothelial cells relative to cells other than human corneal endothelial cells, wherein the second positive affinity reagent differs from the first positive affinity reagent.
    • Aspect 40. The composition of aspect 39, comprising human corneal endothelial cells to which the second positive affinity reagent is bound.
    • Aspect 41. The composition of any of aspects 39-40, wherein the second positive affinity reagent selectively binds to a corneal protein selected from protein products of genes X1 through X26 of Table 2, and may comprise, for example, an antibody or aptamer that binds to a corneal protein selected from protein products of genes X1 through X26 of Table 2, including an antibody or aptamer that binds to one or more proteins selected from SEQ ID NO (1) through SEQ ID NO (58) of Table 2.
    • Aspect 42. The composition of any of aspects 39-40, wherein the second positive affinity reagent comprises an antibody or aptamer that binds to a protein product of gene X5 of Table 2, an antibody or aptamer that binds to a protein product of gene X15 of Table 2, or an antibody or aptamer that binds to a protein product of X25 of Table 2.
    • Aspect 43. The composition of any of aspects 39-40, wherein the second positive affinity reagent comprises (a) an antibody or aptamer that binds to SEQ ID NO (8), (b) an antibody or aptamer that binds to one or more of SEQ ID NO (27), SEQ ID NO (28), SEQ ID NO (29), SEQ ID NO (30) or SEQ ID NO (31), or (c) an antibody or aptamer that binds to one or more of SEQ ID NO (53), SEQ ID NO (54), SEQ ID NO (55), SEQ ID NO (56) or SEQ ID NO (57).
    • Aspect 44. The composition of any of aspects 39-43, wherein the second positive affinity reagent comprises a label.
    • Aspect 45. The composition of any of aspects 31-44, comprising a measurable amount of one or more negative affinity reagents that selectively binds to cells other than human corneal endothelial cells relative to human corneal endothelial cells.
    • Aspect 46. The composition of aspect 45, wherein the one or more negative affinity reagents selectively binds to corneal keratocytes, human corneal endothelial cells of lower utility, or both, relative to human corneal endothelial cells.
    • Aspect 47. The composition of any of aspects 45-46, wherein the one or more negative affinity reagents selectively bind to a corneal protein selected from protein products of genes Y1 through Y23 of Table 2, and may comprise, for example, one or more antibodies that bind to one or more corneal proteins selected from protein products of genes Y1 through Y23 of Table 2, including one or more antibodies or aptamers that bind to one or more proteins selected from SEQ ID NO (59) through SEQ ID NO (96) of Table 2, or the one or more negative affinity reagents selectively bind to a corneal protein selected from protein products of genes Z1 through Z8 of Table 2, and may comprise, for example, one or more antibodies or aptamers that bind to one or more corneal proteins selected from protein products of genes Z1 through Z8 of Table 2, including one or more antibodies or aptamers that bind to one or more proteins selected from SEQ ID NO (97) through SEQ ID NO (109) of Table 2.
    • Aspect 48. The composition of any of aspects 45-46, wherein the one or more negative affinity reagents comprise an antibody or aptamer that binds to a protein product of gene Y6 of Table 2.
    • Aspect 49. The composition of any of aspects 45-46, wherein the one or more negative affinity reagents comprise an antibody or aptamer that binds to one or more of SEQ ID NO (66), SEQ ID NO (67) or SEQ ID NO (68).
    • Aspect 50. The composition of any of aspects 45-49, wherein the one or more negative affinity reagents comprise an antibody or aptamer that is coupled to a label.

These and various other aspects and embodiments and as well as various advantages of the present invention will become immediately apparent to those of ordinary skill in the art upon review of the Detailed Description and appended claims to follow.

BRIEF DESCRIPTION OF THE DRAWINGS

FIGS. 1A-1C are bright field micrographs of HCECs and keratocytes in culture, with FIG. 1A illustrating a HCEC culture having preserved a typical cobblestone morphology (P2-HCEC-Good), FIG. 1B illustrating a HCEC culture having become fibroblastic (P3-HCEC-Fibroblastic), and FIG. 1C illustrating a human corneal keratocyte culture (P2-HCEC-Keratocytes).

FIG. 2 illustrates in bar graph form expression of four surface markers in different corneal cell populations analyzed by flow cytometry.

FIGS. 3A-3C are dual-color fluorescence histograms of HCECs and keratocytes. These dot plots show the differential expression of two surface markers (CD56:CD166, CD56:CD90 and CD56:CAR) in each cell population, with FIG. 3A corresponding to the P2-HCEC-Good culture shown in FIG. 1A, FIG. 3B corresponding to the P3-HCEC-Fibroblastic culture shown in FIG. 1B, and FIG. 3C corresponding to the P2-HCEC-Keratocytes culture shown in FIG. 1C.

FIGS. 4A-4D present Fluorescence profiles illustrating expression of four surface markers, specifically, CD56 (FIG. 4A), CAR (FIG. 4B), CD109 (FIG. 4C) and CD248 (FIG. 4D) in three different HCEC populations, specifically, canonical (good) HCECs, mixed (canonical and fibroblastic) HCECs, and fibroblastic (HCECs), analyzed by flow cytometry.

FIG. 5 illustrates in bar graph form expression of four surface markers in three different HCEC populations analyzed by flow cytometry.

FIGS. 6A-6C illustrates dual-color fluorescence histograms for various pairs of surface markers, specifically, CD56:CD248 (FIG. 6A), CD56:CD109 (FIG. 6B) and CD56:CAR (FIG. 6C) in three different HCEC populations, specifically, canonical HCECs, mixed HCECs and fibroblastic HCECs.

FIG. 7 illustrates trans-endothelial electrical resistance (TEER) as a function of time for cell cultures of three different HCEC populations.

 DETAILED DESCRIPTION

A more complete understanding of the present invention is available by reference to the following detailed description of numerous aspects and embodiments of the invention. The detailed description of the invention which follows is intended to illustrate but not limit the invention.

As noted above, in some aspects, the present disclosure pertains to positive selection processes in which cell populations containing human corneal cells are contacted with one or more positive affinity reagents that selectively bind to HCECs relative to cells other than HCECs (e.g., corneal keratocytes, etc.), including positive affinity reagents that selectively bind to HCECs that are likely to have a higher clinical efficacy relative to the general HCEC population

In other aspects, the present disclosure pertains to negative selection processes in which cell populations containing human corneal cells are contacted with one or more negative affinity reagents that bind selectively bind to cells other than HCECs (e.g., corneal keratocytes, etc.) relative to HCECs.

These negative and positive selection methods may be used independently or in combination with one another, for example, to identifying HCECs, to isolate HCECs and/or to enrich cell populations with HCECs, among other uses.

Cell populations suitable for HCEC enrichment or isolation include those obtained from intact or residual human corneas, which may come, for instance, from embryonic, fetal, pediatric or adult tissue. For example, intact corneas may be subjected to a peel-off step in which the endothelium and its basement membrane (Descemet's membrane) are peeled off the stroma and collected. See Ko-Hua Chen et al., “Transplantation of Adult Human Corneal Endothelium Ex Vivo: A Morphologic Study,” Cornea 20(7): 731-737, 2001. In other embodiments, cell populations may be obtained from residual corneas (e.g., eye tissue remaining after a corneal button has been used for DSAEK).

Tissue from intact and residual corneas may be separated into individual cells by processes such as enzymatic and/or mechanical dissociation. At this step, cells are incubated for a period of time at room temperature or at 37° C. with a single enzyme or a combination of enzymes including some of the following: collagenase, papain, dispase, elastase, trypsin/EDTA, and/or DNAse. Later the tissues are mechanically dissociated using a conventional pipette or a glass pipette to obtain individual cells or cell clumps than can be then expanded in culture. See, e.g., Li W. et al., Invest Ophthalmol Vis Sci 2007; 48: 614; Ishino Y. et al., Invest Ophthalmol Vis Sci 2004; 45: 800; Chen K. H. et al., Cornea 2001; 20: 731.

The medium in which the cells may be suspended will be any medium which maintains the viability of HCECs. Various media are commercially available and may be used including Minimal Essential Medium (MEM), Dulbecco's Modified Eagle Medium (DMEM), Opti-MEMO, Media 199 or M199, Dulbecco's Modified Eagle Medium with Nutrient Mixture F-12 (DMEM/F-12), F99 Ham's F12, SHEM Ham's F12, EGM-2 endothelial growth medium frequently supplemented with serum of human or animal origin, BSA, HSA, growth factors, antioxidants, antibiotics, antimicotic agents, hormones, amino acids, and peptides. Specific examples of media are shown in Table 1 to follow.

TABLE 1 Base Medium Serum Growth Factors & Supplements [M1] 10%  2 ng/ml bFGF DMEM 50 U/ml penicillin 50 μg/ml streptomycin [M2] 8% 20 ng/ml NGF Opti-MEM-I 5 ng/ml EGF 20 μg/ml ascorbic acid 200 mg/L calcium chloride 100 μg/ml pituitary extract 50 μg/ml gentamicin 1x antibiotic/antimycotic 0.08% chondroitin sulphate [M3] 5% 0.5% DMSO SHEM 2 ng/ml EGF Ham's F12 & DMEM 5 μg/ml insulin (1:1 ratio) 5 μg/ml transferrin 5 ng/ml selenium 0.5 μg/ml hydrocortisone 1 nM cholera toxin 50 μg/ml gentamicin 1.25 μg/ml amphotericin B [M4] 5% 20 μg/ml ascorbic acid F99 20 μg/ml bovine insulin Ham's F 12 & M100 2.5 μg/mol transferrin (1:1 ratio) 0.6 ng/ml sodium selentite 10 ng/ml bFGF

Cell cultures from intact and residual corneas contain unwanted contaminant cells which arise from residual non-endothelial tissue (e.g., stroma, epithelium, etc.) that may be present in the sample. In a culture of HCECs, HCECs that are of low cell transplant utility compared to other HCECs of high cell transplant utility may also be considered, in some fashion, “contaminants”.

Cell populations suitable for HCEC enrichment or isolation also include HCEC cultures in which contaminant cells have out-multiplied HCECs or in which HCECs have transformed spontaneously into other types of cells (e.g., keratocytes, etc.). As previously noted, contaminant cells such as keratocytes are particularly undesirable where it is desired to expand an HCEC culture ex vivo, because such cells grow faster than HCECs and can thus take over a cell culture.

Consequently, various aspects of the invention pertain to methods, reagents and kits for separation of HCECs from other cells, particularly, keratocytes and/or HCECs of lower utility. The HCECs are separated from mixtures of cells by techniques that select cells having particular characteristics.

Human corneal endothelial cells may identified or selected (a) through positive cell markers, which are cell markers that are found on the surfaces of HCECs but which are not found on the surfaces of contaminant cells which may be intermixed with HCECs (e.g., positive selection), (b) through negative cell markers, which are cell markers that are found on surfaces of contaminant cells that are intermixed with HCECs and but which are not found on the surfaces of HCECs (e.g., negative selection), and through a combination of positive and negative cell markers.

For example, in the case where whole human corneas are used as a source of endothelial cells, positive cell markers may be selected from corneal proteins which are found in the endothelium (which is formed from HCECs) but which are not found in other corneal tissue (i.e., the stroma and/or the epithelium). Conversely, negative cell markers may be selected from corneal proteins which are found in corneal tissue other than endothelium tissue (i.e., the stroma and/or the epithelium) but which are not found in corneal endothelium.

As another example, in the case where the source of endothelial cells is an endothelium and basement membrane that have been separated from the stroma and epithelium of an intact cornea, positive cell markers may be selected from corneal cell proteins which are found in the endothelium but which are not found in the stroma, while negative cell markers may be selected from corneal cell proteins which are found in the stroma but which are not found in corneal endothelium.

Corneal proteins which may be useful as cell markers in conjunction with the present invention include the suitable proteins selected from those presented in the Table 2 set forth in Appendix A.

Positive cell markers include suitable corneal proteins selected from protein products of genes X1-X26 in Table 2 (e.g., SEQ ID NO (1) through SEQ ID NO (58)) which are present in the corneal endothelium but are not present in the stroma or the epithelium.

Negative cell markers include (a) suitable corneal proteins selected from protein products of genes Y1-Y23 in Table 2 (e.g., SEQ ID NO (59) through SEQ ID NO (96)), which are present in the stroma and in epithelium but are not present in the endothelium and (b) suitable corneal proteins selected from protein products of genes Z1-Z8 in Table 2 (e.g., SEQ ID NO (97) through SEQ ID NO (109)), which are present in the stroma but are not present in the corneal endothelium (or epithelium).

As previously noted, in some aspects, the present disclosure pertains to (a) positive selection processes in which cell populations containing human corneal cells are contacted with one, two, three, four or more positive affinity reagents that selectively bind to HCECs relative to cells other than HCECs (e.g., corneal keratocytes, etc.), (b) negative selection processes in which cell populations containing human corneal cells are contacted with one, two, three, four or more negative affinity reagents that selectively bind to cells other than HCECs (e.g., corneal keratocytes, etc.) relative to HCECs, and (c) combinations of (a) and (b).

For this purpose, affinity reagents are employed which preferentially bind to various corneal proteins. Positive affinity reagents are those that preferentially bind to positive cell markers associated with HCECs while negative affinity reagents are those that preferably bind to negative cell markers associated with contaminant cells other than HCECs.

Various positive cell markers are described above and include corneal proteins which are found in the endothelium (which is formed from HCECs) but which are not found in other corneal tissue (i.e., the stroma and/or the epithelium). Various negative cell markers are also described above and include corneal proteins which are found in corneal tissue other than endothelium (i.e., the stroma and/or the epithelium) but which are not found in corneal endothelium.

Those skilled in the art will recognize that suitable negative and positive affinity reagents can be employed in any order and/or in any combination.

Affinity reagents suitable for use in the present disclosure may comprise any species which selectively binds to a given surface marker, including positive affinity reagents which selectively bind to positive cell markers and negative affinity reagents which selectively bind to negative cell markers.

Especially useful affinity reagents for the practice of the invention are antibodies (also referred to herein as “affinity antibodies”), nucleic acid aptamers and other engineered forms of protein scaffolds. Antibodies include whole antibodies and antibody fragments, e.g. Fab, F(ab′)2, light or heavy chain fragments, etc.

Affinity antibodies selected for use will have a low level of non-specific interactions.

Affinity antibodies may be polyclonal or monoclonal and, where not commercially available, may be readily produced by techniques known to those skilled in the art.

For instance, affinity antibodies to a given corneal protein may be obtained by immunizing a xenogeneic immunocompetent mammalian host (including murine, rodentia, lagomorpha, ovine, porcine, bovine, etc.) with the corneal protein of interest. Immunizations are performed in accordance with conventional techniques, where the corneal proteins may be injected subcutaneously, intramuscularly, intraperitoneally, intravascularly, etc., over a course of one or more injections. After completion of the immunization schedule, the antiserum may be harvested in accordance with conventional methods to provide polygonal antisera specific for the corneal protein of interest. Lymphocytes may also be harvested from the appropriate lymphoid tissue, e.g. spleen, draining lymph node, etc., and fused with an appropriate fusion partner, for example, a myeloma line, producing a hybridoma secreting a specific monoclonal antibody. Screening clones of hybridomas for the antigenic specificity of interest is performed in accordance with conventional methods.

In numerous embodiments, affinity antibodies are coupled to a suitable substrate, for example, a label or a solid matrix. Labels include magnetic labels such as magnetic beads or micro or nanoparticles including superparamagnetic nanoparticles, which allow for ease of separation. Labels also include biotin, which binds with high affinity to avidin or streptavidin. Labels further include fluorochromes, which can be used with flow cytometry, e.g., fluorescence activated cell sorting (FACS), or the like, to allow for ease of separation of a particular cell type. Fluorescence activated cell sorters have varying degrees of sophistication, such as multiple color channels, low angle and obtuse light scattering detecting channels, impedance channels, etc. Fluorochromes include phycobiliproteins, e.g., phycoerythrin and allophycocyanins, fluorescein and Texas red, cy7 and cy5, among others. Multiple antibodies each with an affinity to a particular corneal protein may each be labeled with a different fluorochrome, to permit independent sorting (multi-color analyses) for each associated cell protein.

Cell selection may also be achieved by “panning” with an affinity antibody attached to a solid matrix, e.g. a plate, an immobilized bead, and so forth. For example, an affinity antibody that has specificity for a particular corneal protein may be bound to a solid matrix and corneal cells displaying that particular corneal protein can be captured by the immobilized antibody while the other cells remain in suspension and can be removed.

Any sorting technique may be employed which is not unduly detrimental to the viability of the selected cells. Combinations of the above techniques may be used.

The precise method for coupling an antibody to a given substrate (e.g., a label, solid matrix, etc.) is not critical to the practice of the present disclosure, and a number of alternatives are known in the art. For example, affinity antibodies may directly or indirectly be coupled to a substrate. Direct coupling to a substrate can be achieved by use of various chemical linking groups, as known in the art. For example, an antibody can be coupled to a substrate through side chain amino or sulfhydryl groups and heterofunctional cross-linking reagents. Many heterofunctional compounds are available for linking to various entities. Specific examples include 3-(2-pyridyldithio)propionic acid N-hydroxysuccinimide ester (SPDP) or 4-(N-maleimidomethyl)-cyclohexane-1-carboxylic acid N-hydroxysuccinimide ester (SMCC), which can react with a reactive sulfhydryl group on the antibody and a reactive amino group on the substrate.

Alternatively, affinity antibodies can be indirectly coupled to a substrate via a hapten or a secondary antibody. For instance, the antibody may be directly conjugated to a hapten, and hapten-specific binding species may be conjugated to the substrate. Suitable haptens include digoxin, digoxigenin, FITC, dinitrophenyl, nitrophenyl, avidin, streptavidin, biotin, etc. For example, an antibody may be coupled to one member of a high affinity binding system (e.g., biotin) and another member of the high affinity binding system (e.g., avidin) attached to a substrate. Methods for conjugation of a hapten to a protein are known in the art, and kits for such conjugations are commercially available. The secondary antibody may be directly or indirectly bound to the substrate.

During cell separation, coupled antibodies may be combined with a suspension of cells and incubated for a period of time sufficient for the antibodies to bind to proteins on the cells. The amount of antibody necessary to bind a particular cell subset may be empirically determined by performing a test separation and analysis. The cells and antibodies are incubated for a period of time sufficient for binding to occur.

The medium in which the cells are separated will be any medium which maintains the viability of the cells. Various media are commercially available and include those listed above.

Coupled affinity antibodies include coupled positive affinity antibodies specific for the corneal proteins which are present on human corneal endothelial cells and which are not present on contaminant cells such as stromal and/or epithelial cells (for positive selection) and coupled negative affinity antibodies specific for corneal proteins which are present on contaminant cells such as stromal and/or epithelial cells and which are not present on human corneal endothelial cells (negative selection).

Once the antibody is bound to the cell, the bound cells are separated in accordance with the specific antibody preparation. For example, FACS separation may be used with fluorochrome labeled antibodies, immunomagnetic selection may be used with magnetic-labeled antibodies, “panning” may be employed with immobilized antibodies, and so forth.

Cells may be separated from affinity antibodies using known techniques, as desired. As a specific example, where an antibody in an immunopanning process is a positive selection antibody, the matrix with attached endothelial cells may be washed to remove unbound cells and the endothelial cells released using a suitable technique (e.g., trypsin digest).

While various specific embodiments employing antibodies as affinity reagents are specifically described herein, it is to be understood that other affinity reagents for binding positive or negative cell markers can be used in the same fashion, including nucleic acid aptamers and other engineered forms of protein scaffolds. Aptamers are synthetic oligonucleotides selected from pools of random-sequence oligonucleotides which bind to a wide range of biomolecular targets with high affinity and specificity. See, e.g., J. Wang and G. Li, “Aptamers against cell surface receptors: selection, modification and application,” Curr Med Chem. 2011; 18(27):4107-16.

The separated cells may be collected in any appropriate medium that maintains the viability of the cells.

Cell populations enriched with HCECs may thus be achieved in this manner. The HCEC population may constitute 50% or more of the cells in the cell composition, preferably at 75% or more of the cells in the cell composition, more preferably at 90% or more of the cells in the cell composition, and may be as many as 95% or more (e.g. substantially pure) of the cells in the cell population. Conversely, the cell populations may contain up to 50% of cells other than HCECs (e.g., corneal keratocytes, etc.), for instance 50% or less of such cells, preferably 25% or less of such cells, more preferably 10% or less of such cells, and may be as few as 5% or less of such cells.

The enriched cell population may be used immediately or stored. For example, at room temperature, at 4° C., at 37° C. or the cells may be frozen at liquid nitrogen temperatures and stored for long periods of time.

In certain embodiments, the enriched cells may be further expanded in vitro by adding culture media as described widely in the literature. See, e.g., Li W et al., Invest Ophthalmol Vis Sci 2007; 48: 614.; Ishino Y et al., Invest Ophthalmol Vis Sci 2004; 45: 800; Chen K H et al., Cornea 2001; 20: 731.

The enriched HCEC compositions thus obtained have a variety of uses in clinical therapy, research, development, and commercial purposes.

For example, for therapeutic purposes, human corneal endothelial cells may be ocularly administered to an eye of a patient in order to treat corneal endothelial cell loss or dysfunction.

Other aspects of the invention pertain to kits for conducting cell separations as described herein. Such kits may include any combination of the following, among other elements: (a) one, two, three or more positive affinity reagents, each of which may be, for example, in the form of a positive affinity antibody attached to a suitable substrate such as a solid matrix (e.g. a plate, immobilized bead, etc.) or label (e.g., magnetic label, fluorescent label, etc.), (b) one, two, three or more unlabeled positive affinity antibodies, which the end user could label using standard methods, choosing their preferred labels (e.g., fluorophores, haptens, etc.), (c) one, two, three or more negative affinity reagents, each of which may be, for example, in the form of a negative affinity antibody attached to a suitable substrate such as a solid matrix (e.g. a plate, immobilized bead, etc.) or label (e.g., magnetic label, fluorescent label, etc.), (d) or one, two, three or more unlabeled negative affinity antibodies, which the end user could label using standard methods, choosing their preferred labels (e.g., fluorophores, haptens, etc.); (e) a combination of (a) and (c); (f) a combination of (b) and (d); (g) packaging; (h) printed materials with one or more of the following: (i) storage information and (ii) instructions regarding how to use the materials contained in the kit (e.g., positive affinity reagents, negative affinity reagents, a combination of antibodies for sequential use, etc.).

Example 1

HCECs were isolated from cadaveric donor corneas (Tampa Lions Eye Bank) and cultured and expanded following the method described by Joyce and Zhu in Cornea. 2004 November; 23(8 Suppl):S8-S19. Briefly, the endothelium and Descemet's membrane were peeled off of the stroma and after overnight stabilization at 37° C. in Opti-MEMO media (Gibco, Life Technologies Corp, Carlsbad, CA), supplemented with 8% fetal bovine serum (FBS), they were incubated for 1 hr at 37° C. with ethylenediaminetetraacetic acid (EDTA) to loosen up the cell-cell interactions. Cells were then mechanically dissociated to obtain a single-cell suspension, they were seeded onto FNC-coated culture wells and labeled as “P0” (passage zero). After reaching confluency, they were trypsinized and further expanded into more wells to increase their number. After one or two rounds of expansion, cells were collected and incubated with different antibodies as indicated below. Keratocytes were also obtained from cadaveric donor corneas using the method described by Stramer et al. in “Monoclonal antibody (3G5)-defined ganglioside: cell surface marker of corneal keratocytes,” Invest. Ophthalmol. Vis. Sci. 2004 vol. 45 no. 3 807-812. While one of the HCEC cultures preserved its typical cobblestone morphology at passage 2 (FIG. 1A), a second culture underwent endothelial-to-mesenchymal transition during passage 3 (P3) and the cells became fibroblastic (FIG. 1B). Such cells are generally referred to herein as human corneal endothelial cells of lower utility (e.g., HCECs that have undergone fibroblastic or mesenchymal transformation, etc.) The keratocyte culture exhibits the typical fibroblastic, elongated cell morphology (FIG. 1C).

HCECs from each culture and keratocytes were collected and incubated with one or more of the following labelled antibodies: (a) APC-CD56 which is a mouse monoclonal antibody against a protein product of gene X15 from Table 2 (referred herein to as CD56 surface protein) coupled to allophycocyanin (BD Biosciences, #555518), (b) PE-CD166, which is a mouse monoclonal antibody against a protein product of gene X1 from Table 2 (referred here to as CD166 surface protein) coupled to phycoerythin (BD Biosciences #559263), (c) FITC-CAR, which is a mouse monoclonal antibody against a protein product of gene X25 from Table 2 (referred to as CAR surface protein) coupled to fluorescein-5-Isothiocyanate (Santa Cruz Biotechnology, Santa Cruz, California, USA #sc-56892) and (d) PECy7-CD90, which is a mouse monoclonal antibody against a protein product of gene Z8 from Table 2 (referred to as CD90 surface protein) coupled to a tandem conjugate of PE (energy donor) which has an excitation wavelength of 565 nm and Cy7 (energy acceptor) which has an emission wavelength of 778 nm) (BD Biosciences #561558).

Expression of surface markers was analyzed using a BD LSR™ II flow cytometry system (BD Biosciences, San Jose, CA). The data shown in FIG. 2 are representative from one experiment. Similar results were obtained upon repeated experimentation. Quantification of the % positive cells for each marker shows that in fibroblastic cultures there is a decreased expression of CD56 and CAR, indicating that antibodies to these proteins may be used in conjunction with positive affinity reagents for “good” HCECs. A significant difference in the expression of CD166 or CD90 was not detected using this particular antibody.

FIGS. 3A-3C are dual-color fluorescence dot plots of the HCECs and keratocytes. These dot plots show the differential expression of two surface markers in each cell population as labeled. The percent of cells positive for an individual marker is shown in FIG. 2.

Example 2

HCECs were isolated from cadaveric donor corneas as described in Example 1. Also as discussed in Example 1, HCEC cultures were obtained (a) which evidenced a typical cobblestone morphology (referred to in this Example 2 as a “canonical” cell culture), (b) where all the cells had undergone an endothelial-to-mesenchymal transition (referred to in this Example as a “fibroblastic” cell culture) and (c) where some HCECs had undergone endothelial-to-mesenchymal transition (referred to in this Example as a “mixed” cell culture).

HCEC surface markers were identified by microarray data, and several with high expression in the endothelium (cultured and freshly dissected) but low expression in stroma were selected to be tested by flow cytometry analysis. In addition to APC-CD56, PE-CD166, FITC-CAR and PECy7-CD90 described in Example 1, also tested were (e) CD109-PE, (i.e., mouse anti-CD109), which is a monoclonal antibody against a protein product of gene Y6 from Table 2 (referred to as CD109 antigen) conjugated to phycoerythrin (PE), BD Biosciences Cat #556040 and (f) CD 248-BV, (i.e., mouse anti-Endosialin), which is an unconjugated monoclonal antibody against a protein product of gene X5 from Table 2 (referred to as CD248 antigen or Endosialin), (Millipore, Temecula, CA, USA, Cat #MAB2626), incubated with Goat polyclonal anti-Mouse IgG secondary antibody conjugated to Brilliant Violent 421 (Biolegend, Inc., San Diego, CA, USA, Cat #405317).

To address whether the expression of those markers in HCECs were affected by the fibroblastic conversion described above, HCEC cultures demonstrating two different morphologies (canonical and fibroblastic) and a corneal keratocyte culture as a control were immunostained for the surface proteins CD90, CAR, CD56 and CD166 (See Example 1, FIG. 2). CD56, CAR, CD109 and CD248 expression was also compared between canonical (good), mixed, and fibroblastic HCECs (see FIGS. 4A-4D and 5). Analysis of the percentage of cells expressing any of the individual markers in canonical and fibroblastic cultures demonstrated that CD56, CAR and CD248 expression was reduced in the fibroblastic culture (see FIG. 5), while CD109 was elevated (see FIG. 5); CD90 and CD166 expression did not significantly change between good/canonical and fibroblastic cultures (see Example 1, FIG. 2). A comparable trend was observed in the keratocyte culture used as control for CD90, CAR, CD56 and CD166 expression (see Example 1, FIG. 2).

Dot plot dual histograms of canonical, mixed and fibroblastic cultures shown in FIGS. 6A-6C demonstrated that canonical HCECs are predominantly CD56, CD248 and CAR positive, and CD109 negative; CD56 and CD248 expression is lost and CD109 expression increases as the culture becomes fibroblastic.

Finally, trans-endothelial electrical resistance (TEER) of cell cultures was measured. HCECs (a) from “good” or “canonical” cultures that expressed high levels of CD56, (b) from mixed cultures and (c) from fibroblastic cultures were plated onto inserts with 0.4 mm pores in 24-well culture plates (Transwell, Corning Costar, Acton, MA) at a density of 20,000 cells/insert and incubated in growth media as described in Example 1. TEER was measured using an EVOM volt-ohm meter with STX2 Electrode (World Precision Instrument, Inc., Sarasota, FL) for up to 65 days after initial plating. TEER measures the apical and basal plasma membrane resistance and the paracellular resistance and is used as an index of monolayer confluence integrity of tight junctions. To calculate final resistance (Ω·cm2), the resistance of blank filters were subtracted from those of filters with cells. Four wells per condition were averaged. HCECs exhibiting a canonical morphology and being CD56-positive demonstrated a superior barrier formation ability measured by TEER (FIG. 7).

Thus, we have identified a panel of surface makers that can be used to characterize a canonical and functionally superior HCEC culture, and may be used as quality control criteria or to potentially separate the best HCEC subpopulations for expansion.

Although various embodiments are specifically illustrated and described herein, it will be appreciated that modifications and variations of the present invention are covered by the above teachings and are within the purview of any appended claims without departing from the spirit and intended scope of the invention.

APPENDIX A

TABLE 2 Gene ID: X1 Gene symbol: ALCAM Gene description: activated leukocyte cell adhesion molecule Unigene: Hs.591293 Genbank: DQ486139 Entrez Gene: 214 Refseq: NM_001627 Protein sequence (SEQ ID NO (1)):    1  meskgasscr llfcllisat vfrpglgwyt vnsaygdtii ipcrldvpqn lmfgkwkyek   61  pdgspvfiaf rsstkksvqy ddvpeykdrl nlsenytlsi snarisdekr fvcmlvtedn  121  vfeaptivkv fkqpskpeiv skalfleteq lkklgdcise dsypdgnitw yrngkvlhpl  181  egavviifkk emdpvtqlyt mtstleyktt kadiqmpftc svtyygpsgq ktihseqavf  241  diyypteqvt iqvlppknai kegdnitlkc lgngnpppee flfylpgqpe girssntytl  301  tdvrrnatgd ykcslidkks miastaitvh yldlslnpsg evtrqigdal pvsctisasr  361  natvvwmkdn irlrsspsfs slhyqdagny vcetalqeve glkkresltl ivegkpqikm  421  tkktdpsgls ktiichvegf pkpaiqwtit gsgsvinqte espyingryy skiiispeen  481  vtltctaenq lertvnslnv saisipehde adeisdenre kvndqakliv givvglllaa  541  lvagvvywly mkksktaskh vnkdlgnmee nkkleennhk tea Gene ID: X2 Gene symbol: ATP1A1 Gene description: sodium/potassium-transporting ATPase subunit alpha-1 Unigene: Hs.371889 Genbank: BC003077 Entrez Gene: 476 Refseq: NM_000701|NM_001160233|NM_001160234 Protein sequence isoform a (SEQ ID NO (2)):    1  mgkgvgrdky epaavseqgd kkgkkgkkdr dmdelkkevs mddhklslde lhrkygtdls   61  rgltsaraae ilardgpnal tpppttpewi kfcrqlfggf smllwigail cflaysiqaa  121  teeepqndnl ylgvvlsavv iitgcfsyyq eaksskimes fknmvpqqal virngekmsi  181  naeevvvgdl vevkggdrip adlriisang ckvdnssltg esepqtrspd ftnenpletr  241  niaffstncv egtargivvy tgdrtvmgri atlasglegg qtpiaaeieh fihiitgvav  301  flgvsffils lileytwlea vifligiiva nvpegllatv tvcltltakr marknclvkn  361  leavetlgst sticsdktgt ltqnrmtvah mwfdnqihea dttenqsgvs fdktsatwla  421  lsriaglcnr avfqanqenl pilkravagd asesallkci elccgsvkem reryakivei  481  pfnstnkyql sihknpntse pqhllvmkga perildress illhgkeqpl deelkdafqn  541  aylelgglge rvlgfchlfl pdeqfpegfq fdtddvnfpi dnlcfvglis midppraavp  601  davgkcrsag ikvimvtgdh pitakaiakg vgiisegnet vediaarlni pvsqvnprda  661  kacvvhgsdl kdmtseqldd ilkyhteivf artspqqkli ivegcqrqga ivavtgdgvn  721  dspalkkadi gvamgiagsd vskqaadmil lddnfasivt gveegrlifd nlkksiaytl  781  tsnipeitpf lifiianipl plgtvtilci dlgtdmvpai slayeqaesd imkrqprnpk  841  tdklvnerli smaygqigmi qalggfftyf vilaengflp ihllglrvdw ddrwindved  901  sygqqwtyeq rkiveftcht affvsivvvq wadlvicktr rnsvfqqgmk nkilifglfe  961  etalaaflsy cpgmgvalrm yplkptwwfc afpysllifv ydevrkliir rrpggwveke 1021  tyy Protein sequence isoform c (SEQ ID NO (3)):    1  mafkvgrdky epaavseqgd kkgkkgkkdr dmdelkkevs mddhklslde lhrkygtdls   61  rgltsaraae ilardgpnal tpppttpewi kfcrqlfggf smllwigail cflaysiqaa  121  teeepqndnl ylgvvlsavv iitgcfsyyq eaksskimes fknmvpqqal virngekmsi  181  naeevvvgdl vevkggdrip adlriisang ckvdnssltg esepqtrspd ftnenpletr  241  niaffstncv egtargivvy tgdrtvmgri atlasglegg qtpiaaeieh fihiitgvav  301  flgvsffils lileytwlea vifligiiva nvpegllatv tvcltltakr marknclvkn  361  leavetlgst sticsdktgt ltqnrmtvah mwfdnqihea dttenqsgvs fdktsatwla  421  lsriaglcnr avfqanqenl pilkravagd asesallkci elccgsvkem reryakivei  481  pfnstnkyql sihknpntse pqhllvmkga perildress illhgkeqpl deelkdafqn  541  aylelgglge rvlgfchifl pdeqfpegfq fdtddvnfpi dnlcfvglis midppraavp  601  davgkcrsag ikvimvtgdh pitakaiakg vgiisegnet vediaarlni pvsqvnprda  661  kacvvhgsdl kdmtseqldd ilkyhteivf artspqqkli ivegcqrqga ivavtgdgvn  721  dspalkkadi gvamgiagsd vskqaadmil lddnfasivt gveegrlifd nlkksiaytl  781  tsnipeitpf lifiianipl plgtvtilci dlgtdmvpai slayeqaesd imkrqprnpk  841  tdklvnerli smaygqigmi qalggfftyf vilaengflp ihllglrvdw ddrwindved  901  sygqqwtyeq rkiveftcht affvsivvvq wadlvicktr rnsvfqqgmk nkilifglfe  961  etalaaflsy cpgmgvalrm yplkptwwfc afpysllifv ydevrkliir rrpggwveke 1021  tyy Protein sequence isoform d (SEQ ID NO (4)):    1  mdelkkevsm ddhklsldel hrkygtdlsr gltsaraaei lardgpnalt pppttpewik   61  fcrqlfggfs mllwigailc flaysiqaat eeepqndnly lgvvlsavvi itgcfsyyqe  121  aksskimesf knmvpqqalv irngekmsin aeevvvgdlv evkggdripa dlriisangc  181  kvdnssltge sepqtrspdf tnenpletrn iaffstncve gtargivvyt gdrtvmgria  241  tlasgleggq tpiaaeiehf ihiitgvavf lgvsffilsl ileytwleav ifligiivan  301  vpegllatvt vcltltakrm arknclvknl eavetlgsts ticsdktgtl tqnrmtvahm  361  wfdnqihead ttenqsgvsf dktsatwlal sriaglcnra vfqanqenlp ilkravagda  421  sesallkcie lccgsvkemr eryakiveip fnstnkyqls ihknpntsep qhllvmkgap  481  erildrcssi llhgkeqpld eelkdafqna ylelgglger vlgfchlflp deqfpegfqf  541  dtddvnfpid nlcfvglism idppraavpd avgkcrsagi kvimvtgdhp itakaiakgv  601  giisegnetv ediaarlnip vsqvnprdak acvvhgsdlk dmtseqlddi lkyhteivfa  661  rtspqqklii vegcqrqgai vavtgdgvnd spalkkadig vamgiagsdv skqaadmill  721  ddnfasivtg veegrlifdn lkksiaytlt snipeitpfl ifiianiplp lgtvtilcid  781  lgtdmvpais layeqaesdi mkrqprnpkt dklvnerlis maygqigmiq alggfftyfv  841  ilaengflpi hllglrvdwd drwindveds ygqqwtyeqr kiveftchta ffvsivvvqw  901  adlvicktrr nsvfqqgmkn kilifglfee talaaflsyc pgmgvalrmy plkptwwfca  961  fpysllifvy devrkliirr rpggwveket yy Gene ID: X3 Gene symbol: CD200 Gene description: CD 200 molecule Unigene: Hs.79015 Genbank: AK297194|AF063591|BC022522|BC031103|AY603771|AK293399 Entrez Gene: 4345 Refseq: NM_001004196|NM_005944 Protein sequence isoform b (SEQ ID NO (5)):    1  merltltrti ggplltatll gkttindyqv irmpfshlst yslvwvmaav vlctaqvqvv   61  tqdereqlyt paslkcslqn aqealivtwq kkkavspenm vtfsenhgvv iqpaykdkin  121  itqlglqnst itfwnitled egcymclfnt fgfgkisgta cltvyvqpiv slhykfsedh  181  lnitcsatar papmvfwkvp rsgienstvt lshpngttsv tsilhikdpk nqvgkevicq  241  vlhlgtvtdf kqtvnkgywf svplllsivs lvillvlisi llywkrhrnq drep Protein sequence isoform a (SEQ ID NO (6)):    1  merlvirmpf shlstyslvw vmaavvlcta qvqvvtqder eqlytpaslk cslqnaqeal   61  ivtwqkkkav spenmvtfse nhgvviqpay kdkinitqlg lqnstitfwn itledegcym  121  clfntfgfgk isgtacltvy vqpivslhyk fsedhlnitc satarpapmv fwkvprsgie  181  nstvtlshpn gttsvtsilh ikdpknqvgk evicqvlhlg tvtdfkqtvn kgywfsvpll  241  lsivslvill vlisillywk rhrnqdrep Gene ID: X4 Gene symbol: LAMB1 Gene description: laminin, beta 1 Unigene: Hs.650585 Genbank: M61916 Entrez Gene: 3912 Refseq: NM_002291 Protein sequence (SEQ ID NO (7)):    1  mgllqllafs flalcrarvr aqepefsygc aegscypatg dlligraqkl svtstcglhk   61  pepycivshl qedkkcficn sqdpyhetln pdshlienvv ttfapnrlki wwqsengven  121  vtiqldleae fhfthlimtf ktfrpaamli erssdfgktw gvyryfaydc easfpgistg  181  pmkkvddiic dsrysdieps tegevifral dpafkiedpy spriqnllki tnlrikfvkl  241  htlgdnllds rmeirekyyy avydmvvrgn cfcyghasec apvdgfneev egmvhghcmc  301  rhntkglnce lcmdfyhdlp wrpaegrnsn ackkcncneh sischfdmav ylatgnvsgg  361  vcddcqhntm grnceqckpf yyqhperdir dpnfcerctc dpagsqnegi cdsytdfstg  421  liagqcrckl nvegehcdvc kegfydisse dpfgckscac nplgtipggn pcdsetghcy  481  ckrlvtgqhc dqclpehwgl sndldgerpc dcdlggalnn scfaesgqcs crphmigrqc  541  nevepgyyfa tldhylyeae eanlgpgvsi verqyiqdri pswtgagfvr vpegayleff  601  idnipysmey diliryepql pdhwekavit vqrpgripts srcgntipdd dnqvvslspg  661  sryvvlprpv cfekgtnytv rlelpqytss dsdvespytl idslvlmpyc ksldiftvgg  721  sgdgvvtnsa wetfqryrcl ensrsvvktp mtdvcrniif sisallhqtg lacecdpqgs  781  lssvcdpngg qcqcrpnvvg rtcnrcapgt fgfgpsgckp cechlqgsvn afcnpvtgqc  841  hcfqgvyarq cdrclpghwg fpscqpcqcn ghaddcdpvt geclncqdyt mghncercla  901  gyygdpiigs gdherpcpcp dgpdsgrqfa rscyqdpvtl qlacvcdpgy igsreddcas  961  gyfgnpsevg gscqpcqchn nidttdpeac dketgrclkc lyhtegehcq fcrfgyygda 1021  lqqdcrkcvc nylgtvqehc ngsdcqcdka tgqclclpnv igqncdrcap ntwqlasgtg 1081  cdpcncnaah sfgpscneft gqcqcmpgfg grtcsecqel fwgdpdvecr acdcdprgie 1141  tpqcdqstgq cvcvegvegp redkctrgys gvfpdctpch qcfalwdvii aeltnrthrf 1201  lekakalkis gvigpyretv dsverkvsei kdilaqspaa eplknignlf eeaeklikdv 1261  temmaqvevk lsdttsqsns takeldslqt eaesldntvk elaeqlefik nsdirgalds 1321  itkyfqmsle aeervnastt epnstveqsa lmrdrvedvm meresqfkek qeeqarllde 1381  lagklqsldl saaaemtcgt ppgascsete cggpncrtde gerkcggpgc gglvtvahna 1441  wqkamdldqd vlsalaeveq lskmvseakl radeakqsae dillktnatk ekmdksneel 1501  rnlikqirnf ltqdsadlds ieavanevlk mempstpqql qnltedirer veslsqvevi 1561  lqhsaadiar aemlleeakr asksatdvkv tadmvkeale eaekaqvaae kaikqadedi 1621  qgtqnlltsi esetaaseet lfnasqrise lernveelkr kaaqnsgeae yiekvvytvk 1681  qsaedvkktl dgeldekykk venliakkte esadarrkae mlqneaktll aqansklqll 1741  kdlerkyedn qryledkaqe larlegevrs llkdisqkva vystcl Gene ID: X5 Gene symbol: CD248 Gene description: endosialin Unigene: Hs.195727 Genbank: AF279142 Entrez Gene: 57124 Refseq: NM_020404 Protein sequence (SEQ ID NO (8)):    1  mllrlllawa aagptlgqdp waaepraacg psscyalfpr rrtfleawra crelggdlat   61  prtpeeaqrv dslvgagpas rllwiglqrq arqcqlqrpl rgftwttgdq dtaftnwaqp  121  asggpcpaqr cvaleasgeh rwlegsctla vdgylcqfgf egacpalqde agqagpavyt  181  tpfhlvstef ewlpfgsvaa vqcqagrgas llcvkqpegg vgwsragplc lgtgcspdng  241  gcehecveev dghvscrcte gfrlaadgrs cedpcaqapc eqqcepggpq gyschcrlgf  301  rpaeddphrc vdtdecqiag vcqqmcvnyv ggfecycseg heleadgisc spagamgaqa  361  sqdlgdelld dgedeedede awkafnggwt empgilwmep tqppdfalay rpsfpedrep  421  qipypeptwp pplsaprvpy hssvlsvtrp vvvsathptl psahqppvip athpalsrdh  481  qipviaanyp dlpsayqpgi lsvshsaqpp ahqppmistk ypelfpahqs pmfpdtrvag  541  tqttthlpgi ppnhaplvtt lgaqlppqap dalvlrtqat qlpiiptaqp sltttsrspv  601  spahqisvpa atqpaalptl lpsqsptnqt spispthphs kapqipredg pspklalwlp  661  spaptaapta lgeaglaehs qrddrwllva llvptcvflv vllalgivyc trcgphapnk  721  ritdcyrwvi hagsksptep mpprgsltgv qtcrtsv Gene ID: X6 Gene symbol: COL4A6 Gene description: collagen, type IV, alpha 6 Unigene: Hs.145586 Genbank: D21337 Entrez Gene: 1288 Refseq: NM_033641|NM_001847 Protein sequence isoform b (SEQ ID NO (9)):    1  mhpglwlllv tlclteelaa ageksygkpc ggqdcsgscq cfpekgargr pgpigiqgpt   61  gpqgftgstg lsglkgergf pgllgpygpk gdkgpmgvpg flgingipgh pgqpgprgpp  121  gldgcngtqg avgfpgpdgy pgllgppglp gqkgskgdpv lapgsfkgmk gdpglpgldg  181  itgpqgapgf pgavgpagpp glqgppgppg plgpdgnmgl gfqgekgvkg dvglpgpagp  241  ppstgelefm gfpkgkkgsk gepgpkgfpg isgppgfpgl gttgekgekg ekgipglpgp  301  rgpmgsegvq gppgqqgkkg tlgfpglngf qgiegqkgdi glpgpdvfid idgavisgnp  361  gdpgvpglpg lkgdegiqgl rgpsgvpglp alsgvpgalg pqgfpglkgd qgnpgrttig  421  aaglpgrdgl pgppgppgpp spefetetlh nkesgfpglr geqgpkgnlg lkgikgdsgf  481  cacdggvpnt gppgepgppg pwgliglpgl kgargdrgsg gaqgpagapg lvgplgpsgp  541  kgkkgepils tiqgmpgdrg dsgsqgfrgv igepgkdgvp glpglpglpg dggqgfpgek  601  glpglpgekg hpgppglpgn glpglpgprg lpgdkgkdgl pgqqglpgsk gitlpciipg  661  sygpsgfpgt pgfpgpkgsr glpgtpgqpg ssgskgepgs pglvhlpelp gfpgprgekg  721  lpgfpglpgk dglpgmigsp glpgskgatg difgaengap geqglqgltg hkgflgdsgl  781  pglkgvhgkp gllgpkgerg spgtpgqvgq pgtpgssgpy gikgksglpg apgfpgisgh  841  pgkkgtrgkk gppgsivkkg lpglkglpgn pglvglkgsp gspgvaglpa lsgpkgekgs  901  vgfvgfpgip glpgipgtrg lkgipgstgk mgpsgragtp gekgdrgnpg pvgipsprrp  961  msnlwlkgdk gsqgsagsng fpgprgdkge agrpgppglp gapglpgiik gvsgkpgppg 1021  fmgirglpgl kgssgitgfp gmpgesgsqg irgspglpga sglpglkgdn gqtveisgsp 1081  gpkgqpgesg fkgtkgrdgl ignigfpgnk gedgkvgvsg dvglpgapgf pgvagmrgep 1141  glpgssghqg aigplgspgl igpkgfpgfp glhglnglpg tkgthgtpgp sitgvpgpag 1201  lpgpkgekgy pgigigapgk pglrgqkgdr gfpglqgpag lpgapgislp sliagqpgdp 1261  grpgldgerg rpgpagppgp pgpssnqgdt gdpgfpgipg pkgpkgdqgi pgfsglpgel 1321  glkgmrgepg fmgtpgkvgp pgdpgfpgmk gkagprgssg lqgdpgqtpt aeavqvppgp 1381  lglpgidgip gltgdpgaqg pvglqgskgl pgipgkdgps glpgppgalg dpglpglqgp 1441  pgfegapgqq gpfgmpgmpg qsmrvgytlv khsqseqvpp cpigmsqlwv gysllfvegq 1501  ekahnqdlgf agsclprfst mpfiycnine vchyarrndk sywlsttapi pmmpvsqtqi 1561  pqyisrcsvc eapsqaiavh sqditipqcp lgwrslwigy sflmhtaaga egggqslvsp 1621  gscledfrat pfiecsgarg tchyfankys fwlttveerq qfgelpvset lkagqlhtrv 1681  srcqvcmksl Protein sequence isoform a (SEQ ID NO (10)):    1  mlinklwlll vtlclteela aageksygkp cggqdesgsc qcfpekgarg rpgpigiqgp   61  tgpqgftgst glsglkgerg fpgllgpygp kgdkgpmgvp gflgingipg hpgqpgprgp  121  pgldgcngtq gavgfpgpdg ypgllgppgl pgqkgskgdp vlapgsfkgm kgdpglpgld  181  gitgpqgapg fpgavgpagp pglqgppgpp gplgpdgnmg lgfqgekgvk gdvglpgpag  241  pppstgelef mgfpkgkkgs kgepgpkgfp gisgppgfpg lgttgekgek gekgipglpg  301  prgpmgsegv qgppgqqgkk gtlgfpglng fqgiegqkgd iglpgpdvfi didgavisgn  361  pgdpgvpglp glkgdegiqg lrgpsgvpgl palsgvpgal gpqgfpglkg dqgnpgrtti  421  gaaglpgrdg lpgppgppgp pspefetetl hnkesgfpgl rgeqgpkgnl glkgikgdsg  481  fcacdggvpn tgppgepgpp gpwgliglpg lkgargdrgs ggaqgpagap glvgplgpsg  541  pkgkkgepil stiqgmpgdr gdsgsqgfrg vigepgkdgv pglpglpglp gdggqgfpge  601  kglpglpgek ghpgppglpg nglpglpgpr glpgdkgkdg lpgqqglpgs kgitlpciip  661  gsygpsgfpg tpgfpgpkgs rglpgtpgqp gssgskgepg spglvhlpel pgfpgprgek  721  glpgfpglpg kdglpgmigs pglpgskgat gdifgaenga pgeqglqglt ghkgflgdsg  781  lpglkgvhgk pgllgpkger gspgtpgqvg qpgtpgssgp ygikgksglp gapgfpgisg  841  hpgkkgtrgk kgppgsivkk glpglkglpg npglvglkgs pgspgvaglp alsgpkgekg  901  svgfvgfpgi pglpgipgtr glkgipgstg kmgpsgragt pgekgdrgnp gpvgipsprr  961  pmsnlwlkgd kgsqgsagsn gfpgprgdkg eagrpgppgl pgapglpgii kgvsgkpgpp 1021  gfmgirglpg lkgssgitgf pgmpgesgsq girgspglpg asglpglkgd ngqtveisgs 1081  pgpkgqpges gfkgtkgrdg lignigfpgn kgedgkvgvs gdvglpgapg fpgvagmrge 1141  pglpgssghq gaigplgspg ligpkgfpgf pglhginglp gtkgthgtpg psitgvpgpa 1201  glpgpkgekg ypgigigapg kpglrgqkgd rgfpglqgpa glpgapgisl psliagqpgd 1261  pgrpgldger grpgpagppg ppgpssnqgd tgdpgfpgip gpkgpkgdqg ipgfsglpge 1321  lglkgmrgep gfmgtpgkvg ppgdpgfpgm kgkagprgss glqgdpgqtp taeavqvppg 1381  plglpgidgi pgltgdpgaq gpvglqgskg lpgipgkdgp sglpgppgal gdpglpglqg 1441  ppgfegapgq qgpfgmpgmp gqsmrvgytl vkhsqseqvp pcpigmsqlw vgysllfveg 1501  qekahnqdlg fagsclprfs tmpfiycnin evchyarrnd ksywlsttap ipmmpvsqtq 1561  ipqyisrcsv ceapsqaiav hsqditipqc plgwrslwig ysflmhtaag aegggqslvs 1621  pgscledfra tpfiecsgar gtchyfanky sfwlttveer qqfgelpvse tlkagqlhtr 1681  vsrcqvcmks l Gene ID: X7 Gene symbol: PCDH7 Gene description: protocadherin 7 Unigene: Hs.479439|Hs.724529 Genbank: AB006755 Entrez Gene: 5099 Refseq: NM_032456|NM_002589|NM_032457|NM_001173523 Protein sequence isoform b (SEQ ID NO (11)):    1  mlrmrtagwa rgwclgccll lplslslaaa kqllryrlae egpadvrign vasdlgivtg   61  sgevtfsles gseylkidnl tgelstserr idreklpqcq mifdenecfl dfevsvigps  121  qswvdlfegq vivldindnt ptfpspvltl tveenrpvgt lyllptatdr dfgrngiery  181  ellqepgggg sggesrraga adsapypggg gngasgggsg gskrrldase ggggtnpggr  241  ssvfelqvad tpdgekqpql ivkgaldreq rdsyeltlrv rdggdpprss qailmvlitd  301  vndnsprfek svyeadlaen sapgtpilql raadldvgvn gqieyvfgaa tesvrrllrl  361  detsgwlsvl hridreevnq lrftvmardr gqppktdkat vvlnikdend nvpsieirki  421  griplkdgva nvaedvlvdt pialvqvsdr dqgengvvtc twvgdvpfql kpasdtegdq  481  nkkkyflhts tpldyeatre fnvvivavds gspslssnns livkvgdtnd nppmfgqsvv  541  evyfpennip gervatvlat dadsgknaei aysldssvmg ifaidpdsgd ilvntvldre  601  qtdryefkvn akdkgipvlq gsttvivqva dkndndpkfm qdvftfyvke nlqpnspvgm  661  vtvmdadkgr naemslyiee nnnifsiend tgtiystmsf drehqttytf rvkavdggdp  721  prsatatvsl fvmdendnap tvtlpknisy tllppssnvr tvvatvlatd sddginadln  781  ysivggnpfk lfeidptsgv vslvgkltqk hyglhrlvvq vndsgqpsqs tttlvhvfvn  841  esvsnataid sqiarslhip ltqdiagdps yeiskqrlsi vigvvagimt viliilivvm  901  arycrsknkn gyeagkkdhe dfftpqqhdk skkpkkdkkn kkskqplyss ivtveaskpn  961  gqrydsvnek lsdspsmgry rsvnggpgsp dlarhyksss plptvqlhpq sptagkkhqa 1021  vqdlppantf vgagdnisig sdhcseyscq tnnkyskqvr cipnifkypr eg Protein sequence isoform a (SEQ ID NO (12)):    1  mlrmrtagwa rgwclgccll lplslslaaa kqllrynlae egpadvrign vasdlgivtg   61  sgevtfsles gseylkidnl tgelstserr idreklpqcq mifdenecfl dfevsvigps  121  qswvdlfegq vivldindnt ptfpspvltl tveenrpvgt lyllptatdr dfgrngiery  181  ellqepgggg sggesrraga adsapypggg gngasgggsg gskrrldase ggggtnpggr  241  ssvfelqvad tpdgekqpql ivkgaldreq rdsyeltlrv rdggdpprss qailrvlitd  301  vndnsprfek svyeadlaen sapgtpilql raadldvgvn gqieyvfgaa tesvrrllrl  361  detsgwlsvl hridreevnq lrftvmardr gqppktdkat vvlnikdend nvpsieirki  421  griplkdgva nvaedvlvdt pialvqvsdr dqgengvvtc twvgdvpfql kpasdtegdq  481  nkkkyflhts tpldyeatre fnvvivavds gspslssnns livkvgdtnd nppmfgqsvv  541  evyfpennip gervatvlat dadsgknaei aysldssvmg ifaidpdsgd ilvntvldre  601  qtdryefkvn akdkgipvlq gsttvivqva dkndndpkfm qdvftfyvke nlqpnspvgm  661  vtvmdadkgr naemslyiee nnnifsiend tgtiystmsf drehqttytf rvkavdggdp  721  prsatatvsl fvmdendnap tvtlpknisy tllppssnvr tvvatvlatd sddginadln  781  ysivggnpfk lfeidptsgv vslvgkltqk hyglhrlwvq vndsgqpsqs tttlvhvfvn  841  esvsnataid sqiarslhip ltqdiagdps yeiskqrlsi vigvvagimt viliilivvm  901  arycrsknkn gyeagkkdhe dfftpqqhdk skkpkkdkkn kkskqplyss ivtveaskpn  961  gqrydsvnek lsdspsmgry rsvnggpgsp dlarhyksss plptvqlhpq sptagkkhqa 1021  vqdlppantf vgagdnisig sdhcseyscq tnnkyskqmr lhpyitvfg Protein sequence isoform c (SEQ ID NO (13)):    1  mlrmrtagwa rgwclgccll lplslslaaa kqllrynlae egpadvrign vasdlgivtg   61  sgevtfsles gseylkidnl tgelstserr idreklpqcq mifdenecfl dfevsvigps  121  qswvdlfegq vivldindnt ptfpspvltl tveenrpvgt lyllptatdr dfgrngiery  181  ellqepgggg sggesrraga adsapypggg gngasgggsg gskrrldase ggggtnpggr  241  ssvfelqvad tpdgekqpql ivkgaldreq rdsyeltlrv rdggdpprss qailrvlitd  301  vndnsprfek svyeadlaen sapgtpilql raadldvgvn gqieyvfgaa tesvrrllrl  361  detsgwlsvl hridreevnq lrftvmardr gqppktdkat vvlnikdend nvpsieirki  421  griplkdgva nvaedvlvdt pialvqvsdr dqgengvvtc tvvgdvpfql kpasdtegdq  481  nkkkyflhts tpldyeatre fnvvivavds gspslssnns livkvgdtnd nppmfgqsvv  541  evyfpennip gervatvlat dadsgknaei aysldssvmg ifaidpdsgd ilvntvldre  601  qtdryefkvn akdkgipvlq gsttvivqva dkndndpkfm qdvftfyvke nlqpnspvgm  661  vtvmdadkgr naemslyiee nnnifsiend tgtiystmsf drehqttytf rvkavdggdp  721  prsatatvsl fvmdendnap tvtlpknisy tllppssnvr tvvatvlatd sddginadln  781  ysivggnpfk lfeidptsgv vslvgkltqk hyglhrlvvq vndsgqpsqs tttlvhvfvn  841  esvsnataid sqiarslhip ltqdiagdps yeiskqrlsi vigvvagimt viliilivvm  901  arycrsknkn gyeagkkdhe dfftpqqhdk skkpkkdkkn kkskqplyss ivtveaskpn  961  gqrydsvnek lsdspsmgry rsvnggpgsp dlarhyksss plptvqlhpq sptagkkhqa 1021  vqdlppantf vgagdnisig sdhcseyscq tnnkyskqpf rrvtfsvvsq pqdphqgslq 1081  scydsglees etpssksssg prlgalplpe dnyerttpdg svdsrplpdv altgkctrec 1141  deyghsdscw mpvrtsperk ksqpklstfm pvdergsqek langeaaimg drnrnllnkk 1201  ltssyetfsa asfskneean pedipltktg eykpspvntl trrevyl Protein sequence isoform d (SEQ ID NO (14)):    1  mlrmrtagwa rgwclgccll lplslslaaa kqllryrlae egpadvrign vasdlgivtg   61  sgevtfsles gseylkidnl tgelstserr idreklpqcq mifdenecfl dfevsvigps  121  qswvdlfegq vivldindnt ptfpspvltl tveenrpvgt lyllptatdr dfgrngiery  181  ellqepgggg sggesrraga adsapypggg gngasgggsg gskrrldase ggggtnpggr  241  ssvfelqvad tpdgekqpql ivkgaldreq rdsyeltlrv rdggdpprss qailrvlitd  301  vndnsprfek svyeadlaen sapgtpilql raadldvgvn gqieyvfgaa tesvrrllrl  361  detsgwlsvl hridreevnq lrftvmardr gqppktdkat vvlnikdend nvpsieirki  421  griplkdgva nvaedvlvdt pialvqvsdr dqgengvvtc tvvgdvpfql kpasdtegdq  481  nkkkyflhts tpldyeatre fnvvivavds gspslssnns livkvgdtnd nppmfgqswv  541  evyfpennip gervatvlat dadsgknaei aysldssvmg ifaidpdsgd ilvntvldre  601  qtdryefkvn akdkgipvlq gsttvivqva dkndndpkfm qdvftfyvke nlqpnspvgm  661  vtvmdadkgr naemslyiee nnnifsiend tgtiystmsf drehqttytf rvkavdggdp  721  prsatatvsl fvmdendnap tvtlpknisy tllppssnvr tvvatvlatd sddginadln  781  ysivggnpfk lfeidptsgv vslvgkltqk hyglhrlvvq vndsgqpsqs tttlvhvfvn  841  esvsnataid sqiarslhip ltqdiagdps yeiskqrlsi vigvvagimt viliilivvm  901  arycrsknkn gyeagkkdhe dfftpqqhdk skkpkkdkkn kkskqplyss ivtveaskpn  961  gqrydsvnek lsdspsmgry rsvnggpgsp dlarhyksss plptvqlhpq sptagkkhqa 1021  vqdlppantf vgagdnisig sdhcseyscq tnnkyskqpf rrvtfsvvsq pqdphqgslq 1081  scydsglees etpssksssg prlgalplpe dnyerttpdg svgeaehmen dsrplpdval 1141  tgkctrecde yghsdscwmp vrtsperkks qpklstfmpv dergsqekla ngeaaimgdr 1201  nrnllnkklt ssyetfsaas fskneeanpe dipltktgey kpspvntltr revyl Gene ID: X8 Gene symbol: NOG Gene description: noggin Unigene: Hs.248201 Genbank: BC034027 Entrez Gene: 9241 Refseq: NM_005450 Protein sequence (SEQ ID NO (15)):    1  mercpslgvt lyalvvvlgl ratpaggqhy lhirpapsdn lplvdliehp dpifdpkekd   61  lnetllrsll gghydpgfma tsppedrpgg gggaaggaed laeldqllrq rpsgampsei  121  kglefsegla qgkkqrlskk lrrklqmwlw sqtfcpvlya wndlgsrfwp ryvkvgscfs  181  krscsvpegm vckpsksvhl tvlrwrcqrr ggqrcgwipi qypiiseckc sc Gene ID: X9 Gene symbol: SULF1 Gene description: sulfatase 1 Unigene: Hs.409602 Genbank: AF545571 Entrez Gene: 23213 Refseq: NM_001128205|NM_015170|NM_001128206|NM_001128204 Protein sequence (SEQ ID NO (16)):    1  mkysccalvl avlgtellgs lcstvrsprf rgriqqerkn irpniilvit ddqdvelgsl   61  qvmnktrkim ehggatfina fvttpmccps rssmitgkyv hnhnvytnne ncsspswqam  121  heprtfavyl nntgyrtaff gkylneyngs yippgwrewl gliknsrfyn ytvcrngike  181  khgfdyakdy ftdlitnesi nyfkmskrmy phrpvmmvis haaphgpeds apqfsklypn  241  asqhitpsyn yapnmdkhwi mqytgpmlpi hmeftnilqr kriqtlmsvd dsverlynml  301  vetgelenty iiytadhgyh igqfglvkgk smpydfdirv pffirgpsve pgsivpqivl  361  nidlaptild iagldtppdv dgksvlklld pekpgnrfrt nkkakiwrdt flvergkflr  421  kkeesskniq qsnhlpkyer vkelcqqary qtaceqpgqk wqciedtsgk lrihkckgps  481  dlltvrqstr nlyargfhdk dkecscresg yrasrsqrks qrqflrnqgt pkykprfvht  541  rqtrslsvef egeiydinle eeeelqvlqp rniakrhdeg hkgprdlqas sggnrgrmla  601  dssnavgppt tvrvthkcfi lpndsihcer elyqsarawk dhkayidkei ealqdkiknl  661  revrghlkrr kpeecscskq syynkekgvk kqeklkshlh pfkeaaqevd sklqlfkenn  721  rrrkkerkek rrqrkgeecs lpgltcfthd nnhwqtapfw nlgsfcacts snnntywclr  781  tvnethnflf cefatgfley fdmntdpyql tntvhtverg ilnqlhvqlm elrscqgykq  841  cnprpknldv gnkdggsydl hrgqlwdgwe g Gene ID: X10 Gene symbol: SORT1 Gene description: sortilin 1 Unigene: Hs.485195 Genbank: X98248 Entrez Gene: 6272 Refseq: NM_002959|NM_001205228 Protein sequence isoform 1 (SEQ ID NO (17)):    1  merpwgaadg lsrwphglgl llllqllpps tlsqdrldap pppaaplprw sgpigvswgl   61  raaaaggafp rggrwrrsap gedeecgrvr dfvaklannt hqhvfddlrg svslswvgds  121  tgvilvlttf hvplvimtfg qsklyrsedy gknfkditdl inntfirtef gmaigpensg  181  kwvltaevsg gsrggrifrs sdfaknfvqt dlpfhpltqm myspqnsdyl lalstenglw  241  vsknfggkwe eihkavclak wgsdntifft tyangsckad lgalelwrts dlgksfktig  301  vkiysfglgg rflfasvmad kdttrrihvs tdqgdtwsma qlpsvgqeqf ysilaanddm  361  vfmhvdepgd tgfgtiftsd drgivysksl drhlytttgg etdftnvtsl rgvyitsvls  421  ednsiqtmit fdqggrwthl rkpensecda taknknecsl hihasysisq klnvpmapls  481  epnavgivia hgsvgdaisv mvpdvyisdd ggyswtkmle gphyytilds ggiivaiehs  541  srpinvikfs tdegqcwqty tftrdpiyft glasepgars mnisiwgfte sfltsqwvsy  601  tidfkdiler nceekdytiw lahstdpedy edgcilgyke qflrlrkssv cqngrdyvvt  661  kqpsiclcsl edflcdfgyy rpendskcve qpelkghdle fclygreehl ttngyrkipg  721  dkcqggvnpv revkdlkkkc tsnflspekq nsksnsvpii laivglmlvt vvagvlivkk  781  yvcggrflvh rysvlqqhae angvdgvdal dtashtnksg yhddsdedll e Protein sequence isoform 2 (SEQ ID NO (18)):    1  mtfgqsklyr sedygknfkd itdlinntfi rtefgmaigp ensgkvvlta evsggsrggr   61  ifrssdfakn fvqtdlpfhp ltqmmyspqn sdyllalste nglwvsknfg gkweeihkav  121  clakwgsdnt iffttyangs ctdlgalelw rtsdlgksfk tigvkiysfg lggrflfasv  181  madkdttrri hvstdqgdtw smaqlpsvgq eqfysilaan ddmvfmhvde pgdtgfgtif  241  tsddrgivys ksldrhlytt tggetdftnv tslrgvyits vlsednsiqt mitfdqggrw  301  thlrkpense cdataknkne cslhihasys isqklnvpma plsepnavgi viahgsvgda  361  isvmvpdvyi sddggyswtk mlegphyyti ldsggiivai ehssrpinvi kfstdegqcw  421  qtytftrdpi yftglasepg arsmnisiwg ftesfltsqw vsytidfkdi lernceekdy  481  tiwlahstdp edyedgcilg ykeqflrlrk ssveqngrdy vvtkqpsicl csledflcdf  541  gyyrpendsk cveqpelkgh dlefclygre ehlttngyrk ipgdkcqggv npvrevkdlk  601  kkctsnflsp ekqnsksnsv piilaivglm lvtvvagvli vkkyvcggrf lvhrysvlqq  661  haeangvdgv daldtashtn ksgyhddsde dlle Gene ID: X11 Gene symbol: ATP1B1 Gene description: sodium/potassium-transporting ATPase subunit beta-1 Unigene: Hs.291196 Genbank: U16799 Entrez Gene: 481 Refseq: NM_001677 Protein sequence (SEQ ID NO (19)):    1  margkakeeg swkkfiwnse kkeflgrtgg swfkillfyv ifygclagif igtiqvmllt   61  isefkptyqd rvappgltqi pqiqkteisf rpndpksyea yvlnivrfle kykdsaqrdd  121  mifedcgdvp sepkergdfn hergerkvcr fklewlgncs glndetygyk egkpciiikl  181  nrvlgfkpkp pknesletyp vmkynpnvlp vqctgkrded kdkvgnveyf glgnspgfpl  241  qyypyygkll qpkylqplla vqftnltmdt eirieckayg enigysekdr fqgrfdvkie  301  vks Gene ID: X12 Gene symbol: AGRN Gene description: Agrin Unigene: Hs.273330 Genbank: AB191264 Entrez Gene: 375790 Refseq: NM_198576 Protein sequence (SEQ ID NO (20)):    1  magrshpgpl rpllpllvva acvlpgaggt cperalerre eeanwvltgt veeilnvdpv   61  qhtysckvrv wrylkgkdlv areslldggn kvvisgfgdp licdnqvstg dtriffvnpa  121  ppylwpahkn elmlnsslmr itlrnleeve fcvedkpgth ftpvpptppd acrgmlcgfg  181  avcepnaegp grascvckks pcpsvvapvc gsdastysne celqraqcsq qrrirllsrg  241  pcgsrdpcsn vtcsfgstca rsadgltasc lcpatcrgap egtvcgsdga dypgecqllr  301  racarqenvf kkfdgpcdpc qgalpdpsrs crvnprtrrp emllrpescp arqapvcgdd  361  gvtyendcvm grsgaargll lqkvrsgqcq grdqcpeper fnavelsrrg rprescdrvt  421  cdgayrpvca qdgrtydsdc wrqqaecrqq raipskhqgp cdqapspclg vqcafgatca  481  vkngqaacec lqacsslydp vcgsdgvtyg saceleatac tlgreiqvar kgpedregqc  541  rfgalceaet grcvcpsecv alaqpvcgsd ghtypsecml hvhacthqis lhvasagpce  601  tcgdavcafg avcsagqcvc prcehpppgp vcgsdgvtyg sacelreaac lqqtqieear  661  agpceqaecg sggsgsgedg dceqelcrqr ggiwdedsed gpcvcdfscq svpgspvcgs  721  dgvtystece lkkarcesqr glyvaaqgac rgptfaplpp vaplhcaqtp ygccqdnita  781  argvglagcp sacqcnphgs yggtcdpatg qcscrpgvgg lrcdrcepgf wnfrgivtdg  841  rsgctpcscd pqgavrddce qmtglcsckp gvagpkcgqc pdgralgpag ceadasapat  901  caemrcefga reveesgsah cvcpmltcpe anatkvcgsd gvtygnecql ktiacrqglq  961  isiqslgpcq eavapsthpt sasvtvttpg lllsqalpap pgalplapss tahsqttppp 1021  ssrprttasv prttvwpvlt vpptapspap slvasafges gstdgssdee lsgdqeasgg 1081  gsgglepleg ssvatpgppv erascynsal gccsdgktps ldaegsncpa tkvfqgvlel 1141  egvegqelfy tpemadpkse lfgetarsie stlddlfrns dvkkdfrsvr lrdlgpgksv 1201  raivdvhfdp ttafrapdva rallrqiqvs rrrslgvrrp lqehvrfmdf dwfpafitga 1261  tsgaiaagat arattasrlp ssavtpraph pshtsqpvak ttaapttrrp pttapsrvpg 1321  rrppapqqpp kpcdsqpcfh ggtcqdwalg ggftcscpag rggavcekvl gapvpafegr 1381  sflafptira yhtlrlalef ralepqglll yngnargkdf lalalldgrv qlrfdtgsgp 1441  avltsavpve pgqwhrlels rhwrrgtlsv dgetpvlges psgtdglnld tdlfvggvpe 1501  dqaavalert fvgaglrgci rlldvnnqrl elgigpgaat rgsgvgecgd hpclpnpchg 1561  gapcqnleag rfhcqcppgr vgptcadeks pcqpnpchga apcrvlpegg aqcecplgre 1621  gtfcqtasgq dgsgpfladf ngfshlelrg lhtfardlge kmalevvfla rgpsglllyn 1681  gqktdgkgdf vslalrdrrl efrydlgkga avirsrepvt lgawtrvsle rngrkgalrv 1741  gdgprvlges pvphtvinik eplyvggapd fsklaraaav ssgfdgaiql vslggrqllt 1801  pehvlrqvdv tsfaghpctr asghpclnga scvpreaayv clcpggfsgp hcekglveks 1861  agdvdtlafd grtfveylna vtesekalqs nhfelslrte atqglvlwsg kateradyva 1921  laivdghlql synlgsqpvv lrstvpvntn rwlrvvahre qregslqvgn eapvtgsspl 1981  gatqldtdga lwlgglpelp vgpalpkayg tgfvgclrdv vvgrhplhll edavtkpelr 2041  pcptp Gene ID: X13 Gene symbol: APP Gene description: Amyloid beta A4 protein Unigene: Hs.434980 Genbank: BC065529|AF282245|AK298861|AK294534|AK295621|AK296229|AK297412| AK297229|AK295373|BC004369|M16765|AK311717 Entrez Gene: 351 Refseq: NM_000484|NM_201413|NM_001136130|NM_201414|NM_001136129 Protein sequence isoform a (SEQ ID NO (21)):    1  mlpglallll aawtaralev ptdgnaglla epqiamfcgr lnmhmnvqng kwdsdpsgtk   61  tcidtkegil qycqevypel qitnvveanq pvtiqnwckr grkqckthph fvipyrclvg  121  efvsdallvp dkckflhqer mdvcethlhw htvaketcse kstnlhdygm llpcgidkfr  181  gvefvccpla eesdnvdsad aeeddsdvww ggadtdyadg sedkvvevae eeevaeveee  241  eadddedded gdeveeeaee pyeeatertt siattttttt esveevvrev cseqaetgpc  301  ramisrwyfd vtegkcapff yggcggnrnn fdteeycmav cgsamsqsll kttqeplard  361  pvklpttaas tpdavdkyle tpgdenehah fqkakerlea khrermsqvm reweeaerqa  421  knlpkadkka viqhfqekve sleqeaaner qqlvethmar veamlndrrr lalenyital  481  qavpprprhv fnmlkkyvra eqkdrqhtlk hfehvrmvdp kkaaqirsqv mthlrviyer  541  mnqslsllyn vpavaeeiqd evdellqkeq nysddvlanm iseprisygn dalmpsltet  601  kttvellpvn gefslddlqp whsfgadsvp antenevepv darpaadrgl ttrpgsgltn  661  ikteeisevk mdaefrhdsg yevhhqklvf faedvgsnkg aiiglmvggv viatvivitl  721  vmlkkkqyts ihhgvvevda avtpeerhls kmqqngyenp tykffeqmqn Protein sequence isoform b (SEQ ID NO (22)):    1  mlpglallll aawtaralev ptdgnaglla epqiamfcgr lnmhmnvqng kwdsdpsgtk   61  tcidtkegil qycqevypel qitnvveanq pvtiqnwckr grkqckthph fvipyrclvg  121  efvsdallvp dkckflhqer mdvcethlhw htvaketcse kstnlhdygm llpcgidkfr  181  gvefvccpla eesdnvdsad aeeddsdvww ggadtdyadg sedkvvevae eeevaeveee  241  eadddedded gdeveeeaee pyeeatertt siattttttt esveevvrev cseqaetgpc  301  ramisrwyfd vtegkcapff yggcggnrnn fdteeycmav cgsaipttaa stpdavdkyl  361  etpgdeneha hfqkakerle akhrermsqv mreweeaerq aknlpkadkk aviqhfqekv  421  eslegeaane rqqlvethma rveamlndrr rlalenyita lqavpprprh vfnmlkkyvr  481  aeqkdrqhtl khfehvrmvd pkkaaqirsq vmthlrviye rmnqslslly nvpavaeeiq  541  devdellqke qnysddvlan miseprisyg ndalmpslte tkttvellpv ngefslddlq  601  pwhsfgadsv pantenevep vdarpaadrg lttrpgsglt nikteeisev kmdaefrhds  661  gyevhhqklv ffaedvgsnk gaiiglmvgg vviatvivit lvmlkkkqyt sihhgvvevd  721  aavtpeerhl skmqqngyen ptykffeqmq n Protein sequence isoform f (SEQ ID NO (23)):    1  mlpglallll aawtaralev ypelqitnvv eanqpvtiqn wckrgrkqck thphfvipyr   61  clvgefvsda llvpdkckfl hqermdvcet hlhwhtvake tcsekstnlh dygmllpcgi  121  dkfrgvefvc cplaeesdnv dsadaeedds dvwwggadtd yadgsedkvv evaeeeevae  181  veeeeaddde ddedgdevee eaeepyeeat erttsiattt ttttesveev vrevcseqae  241  tgpcramisr wyfdvtegkc apffyggcgg nrnnfdteey cmavcgsams qsllkttqep  301  lardpvklpt taastpdavd kyletpgden ehahfqkake rleakhrerm sqvmreweea  361  erqaknlpka dkkaviqhfq ekvesleqea anerqqlvet hmarveamln drrrlaleny  421  italqavppr prhvfnmlkk yvraeqkdrq htlkhfehvr mvdpkkaaqi rsqvmthlrv  481  iyermnqsls llynvpavae eiqdevdell qkeqnysddv lanmisepri sygndalmps  541  ltetkttvel lpvngefsld dlqpwhsfga dsvpantene vepvdarpaa drglttrpgs  601  gltnikteei sevkmdaefr hdsgyevhhq klvffaedvg snkgaiiglm vggvviatvi  661  vitlvmlkkk qytsihhgvv evdaavtpee rhlskmqqng yenptykffe qmqn Protein sequence isoform c (SEQ ID NO (24)):    1  mlpglallll aawtaralev ptdgnaglla epqiamfcgr lnmhmnvqng kwdsdpsgtk   61  tcidtkegil qycqevypel qitnvveanq pvtiqnwckr grkqckthph fvipyrclvg  121  efvsdallvp dkckflhqer mdvcethlhw htvaketcse kstnlhdygm llpcgidkfr  181  gvefvccpla eesdnvdsad aeeddsdvww ggadtdyadg sedkvvevae eeevaeveee  241  eadddedded gdeveeeaee pyeeatertt siattttttt esveevvrvp ttaastpdav  301  dkyletpgde nehahfqkak erleakhrer msqvmrewee aerqaknlpk adkkaviqhf  361  qekvesleqe aanerqqlve thmarveaml ndrrrlalen yitalqavpp rprhvfnmlk  421  kyvraeqkdr qhtlkhfehv rmvdpkkaaq irsqvmthlr viyermnqsl sllynvpava  481  eeiqdevdel lqkeqnysdd vlanmisepr isygndalmp sltetkttve llpvngefsl  541  ddlqpwhsfg adsvpanten evepvdarpa adrglttrpg sgltniktee isevkmdaef  601  rhdsgyevhh qklvffaedv gsnkgaiigl mvggvviatv ivitlvmlkk kqytsihhgv  661  vevdaavtpe erhlskmqqn gyenptykff eqmqn Protein sequence isoform e (SEQ ID NO (25)):    1  mlpglallll aawtaralev ypelqitnvv eanqpvtiqn wckrgrkqck thphfvipyr   61  clvgefvsda llvpdkckfl hqermdvcet hlhwhtvake tcsekstnlh dygmllpcgi  121  dkfrgvefvc cplaeesdnv dsadaeedds dvwwggadtd yadgsedkvv evaeeeevae  181  veeeeaddde ddedgdevee eaeepyeeat erttsiattt ttttesveev vrvpttaast  241  pdavdkylet pgdenehahf qkakerleak hrermsqvmr eweeaerqak nlpkadkkav  301  iqhfqekves leqeaanerq qlvethmarv eamlndrrrl alenyitalq avpprprhvf  361  nmlkkyvrae qkdrqhtlkh fehvrmvdpk kaaqirsqvm thlrviyerm nqslsllynv  421  pavaeeiqde vdellqkeqn ysddvlanmi seprisygnd almpsltetk ttvellpvng  481  efslddlqpw hsfgadsvpa ntenevepvd arpaadrglt trpgsgltni kteeisevkm  541  daefrhdsgy evhhqklvff aedvgsnkga iiglmvggvv iatvivitlv mlkkkqytsi  601  hhgvvevdaa vtpeerhlsk mqqngyenpt ykffeqmqn Gene ID: X14 Gene symbol: COLEC12 Gene description: Collectin sub-family member 12 Unigene: Hs.464422 Genbank: AB038518 Entrez Gene: 81035 Refseq: NM_130386 Protein sequence (SEQ ID NO (26)):    1  mkddfaeeee vqsfgykrfg iqegtqctkc knnwalkfsi illyilcall titvailgyk   61  vvekmdnvtg gmetsrqtyd dkltavesdl kklgdqtgkk aistnselst frsdildlrq  121  qlreitekts knkdtleklq asgdalvdrq sqlketlenn sflittvnkt lqayngyvtn  181  lqqdtsviqg nlqnqmyshn vvimnlnnln ltqvqqrnli tnlqrsvddt sqaiqriknd  241  fqnlqqvflq akkdtdwlke kvqslqtlaa nnsalakann dtledmnsql nsftgqmeni  301  ttisqaneqn lkdlqdlhkd aenrtaikfn qleerfqlfe tdivniisni sytahhlrtl  361  tsnlnevrtt ctdtltkhtd dltslnntla nirldsvslr mqqdlmrsrl dtevanlsvi  421  meemklvdsk hgqliknfti lqgppgprgp rgdrgsqgpp gptgnkgqkg ekgepgppgp  481  agergpigpa gppgerggkg skgsqgpkgs rgspgkpgpq gpsgdpgppg ppgkeglpgp  541  qgppgfqglq gtvgepgvpg prglpglpgv pgmpgpkgpp gppgpsgavv plalqneptp  601  apedngcpph wknftdkcyy fsvekeifed aklfcedkss hlvfintree qqwikkqmvg  661  reshwigltd serenewkwl dgtspdyknw kagqpdnwgh ghgpgedcag liyagqwndf  721  qcedvnnfic ekdretvlss al Gene ID: X15 Gene symbol: NCAM1 Gene description: Neural cell adhesion molecule 1 Unigene: Hs.503878 Genbank: BC047244 Entrez Gene: 4684 Refseq: NM_000615|NM_001076682|NM_181351|NM_001242608| NM_001242607 Protein sequence isoform 1 (SEQ ID NO (27)):    1  mlqtkdliwt lfflgtavsl qvdivpsqge isvgeskffl cqvagdakdk diswfspnge   61  kltpnqqris vvwnddssst ltiynanidd agiykcvvtg edgseseatv nvkifqklmf  121  knaptpqefr egedavivcd vvsslpptii wkhkgrdvil kkdvrfivls nnylqirgik  181  ktdegtyrce grilargein fkdiqvivnv pptiqarqni vnatanlgqs vtlvcdaegf  241  peptmswtkd geqieqeedd ekyifsddss qltikkvdkn deaeyiciae nkageqdati  301  hlkvfakpki tyvenqtame leeqvtltce asgdpipsit wrtstrniss eektldghmv  361  vrsharvssl tlksiqytda geyictasnt igqdsqsmyl evqyapklqg pvavytwegn  421  qvnitcevfa ypsatiswfr dgqllpssny snikiyntps asylevtpds endfgnynct  481  avnrigqesl efilvqadtp sspsidqvep ysstaqvqfd epeatggvpi lkykaewrav  541  geevwhskwy dakeasmegi vtivglkpet tyavrlaaln gkglgeisaa sefktqpvqg  601  epsapklegq mgedgnsikv nlikqddggs pirhylvryr alssewkpei rlpsgsdhvm  661  lksldwnaey evyvvaenqq gkskaahfvf rtsaqptaip angsptsgls tgaivgiliv  721  ifvlllvvvd itcyflnkcg lfmciavnlc gkagpgakgk dmeegkaafs kdeskepive  781  vrteeertpn hdggkhtepn ettpltepek gpveakpecq etetkpapae vktvpndatq  841  tkeneska Protein sequence isoform 3 (SEQ ID NO (28)):    1  mlqtkdliwt lfflgtavsl qvdivpsqge isvgeskffl cqvagdakdk diswfspnge   61  kltpnqqris vvwnddssst ltiynanidd agiykcvvtg edgseseatv nvkifqklmf  121  knaptpqefr egedavivcd vvsslpptii wkhkgrdvil kkdvrfivls nnylqirgik  181  ktdegtyrce grilargein fkdiqvivnv pptiqarqni vnatanlgqs vtlvcdaegf  241  peptmswtkd geqieqeedd ekyifsddss qltikkvdkn deaeyiciae nkageqdati  301  hlkvfakpki tyvenqtame leeqvtltce asgdpipsit wrtstrniss eektldghmv  361  vrsharvssl tlksiqytda geyictasnt igqdsqsmyl evqyapklqg pvavytwegn  421  qvnitcevfa ypsatiswfr dgqllpssny snikiyntps asylevtpds endfgnynct  481  avnrigqesl efilvqadtp sspsidqvep ysstaqvqfd epeatggvpi lkykaewrav  541  geevwhskwy dakeasmegi vtivglkpet tyavrlaaln gkglgeisaa sefktqpvhs  601  ppppasasss tpvplsppdt twplpalatt epakgepsap klegqmgedg nsikvnlikq  661  ddggspirhy lvryralsse wkpeirlpsg sdhvmlksld wnaeyevyvv aenqqgkska  721  ahfvfrtsaq ptaipatlgg nsasytfvsl lfsavtllll c Protein sequence isoform 2 (SEQ ID NO (29)):    1  mlqtkdliwt lfflgtavsl qvdivpsqge isvgeskffl cqvagdakdk diswfspnge   61  kltpnqqris vvwnddssst ltiynanidd agiykcvvtg edgseseatv nvkifqklmf  121  knaptpqefr egedavivcd vvsslpptii wkhkgrdvil kkdvrfivls nnylqirgik  181  ktdegtyrce grilargein fkdiqvivnv pptiqarqni vnatanlgqs vtlvcdaegf  241  peptmswtkd geqieqeedd ekyifsddss qltikkvdkn deaeyiciae nkageqdati  301  hlkvfakpki tyvenqtame leeqvtltce asgdpipsit wrtstrniss eekaswtrpe  361  kqetldghmv vrsharvssl tlksiqytda geyictasnt igqdsqsmyl evqyapklqg  421  pvavytwegn qvnitcevfa ypsatiswfr dgqllpssny snikiyntps asylevtpds  481  endfgnynct avnrigqesl efilvqadtp sspsidqvep ysstaqvqfd epeatggvpi  541  lkykaewrav geevwhskwy dakeasmegi vtivglkpet tyavrlaaln gkglgeisaa  601  sefktqpvqg epsapklegq mgedgnsikv nlikqddggs pirhylvryr alssewkpei  661  rlpsgsdhvm lksldwnaey evyvvaenqq gkskaahfvf rtsaqptaip angsptsgls  721  tgaivgiliv ifvlllvvvd itcyflnkcg lfmciavnlc gkagpgakgk dmeegkaafs  781  kdeskepive vrteeertpn hdggkhtepn ettpltepek gpveakpecq etetkpapae  841  vktvpndatq tkeneska Protein sequence isoform 4 (SEQ ID NO (30)):    1  mlqtkdliwt lfflgtavsl qvdivpsqge isvgeskffl cqvagdakdk diswfspnge   61  kltpnqqris vvwnddssst ltiynanidd agiykcvvtg edgseseatv nvkifqklmf  121  knaptpqefr egedavivcd vvsslpptii wkhkgrdvil kkdvrfivls nnylqirgik  181  ktdegtyrce grilargein fkdiqvivnv pptiqarqni vnatanlgqs vtlvcdaegf  241  peptmswtkd geqieqeedd ekyifsddss qltikkvdkn deaeyiciae nkageqdati  301  hlkvfakpki tyvenqtame leeqvtltce asgdpipsit wrtstrniss eektldghmv  361  vrsharvssl tlksiqytda geyictasnt igqdsqsmyl evqyapklqg pvavytwegn  421  qvnitcevfa ypsatiswfr dgqllpssny snikiyntps asylevtpds endfgnynct  481  avnrigqesl efilvqadtp sspsidqvep ysstaqvqfd epeatggvpi lkykaewrav  541  geevwhskwy dakeasmegi vtivglkpet tyavrlaaln gkglgeisaa sefktqpvqg  601  epsapklegq mgedgnsikv nlikqddggs pirhylvryr alssewkpei rlpsgsdhvm  661  lksldwnaey evyvvaenqq gkskaahfvf rtsaqptaip atlggnsasy tfvsllfsav  721  tllllc Protein sequence isoform 5 (SEQ ID NO (31)):    1  mlqtkdliwt lfflgtavsl qvdivpsqge isvgeskffl cqvagdakdk diswfspnge   61  kltpnqqris vvwnddssst ltiynanidd agiykcvvtg edgseseatv nvkifqklmf  121  knaptpqefr egedavivcd vvsslpptii wkhkgrdvil kkdvrfivls nnylqirgik  181  ktdegtyrce grilargein fkdiqvivnv pptiqarqni vnatanlgqs vtlvcdaegf  241  peptmswtkd geqieqeedd ekyifsddss qltikkvdkn deaeyiciae nkageqdati  301  hlkvfakpki tyvenqtame leeqvtltce asgdpipsit wrtstrniss eekaswtrpe  361  kqevhapwnw qvgrqkgqag sagfpgshet ldghmvvrsh arvssltlks iqytdageyi  421  ctasntigqd sqsmylevqy apklqgpvav ytwegnqvni tcevfaypsa tiswfrdgql  481  lpssnysnik iyntpsasyl evtpdsendf gnynctavnr igqeslefil vqadtpssps  541  idqvepysst aqvqfdepea tggvpilkyk aewravgeev whskwydake asmegivtiv  601  glkpettyav rlaalngkgl geisaasefk tqpvqgepsa pklegqmged gnsikvnlik  661  qddggspirh ylvryralss ewkpeirlps gsdhvmlksl dwnaeyevyv vaenqqgksk  721  aahfvfrtsa qptaipangs ptsglstgai vgilivifvl llvvvditcy flnkcglfmc  781  iavnlcgkag pgakgkdmee gkaafskdes kepivevrte eertpnhdgg khtepnettp  841  ltepekgpve akpecqetet kpapaevktv pndatqtken eska Gene ID: X16 Gene symbol: NRP2 Gene description: Neuropilin-2 Unigene: Hs.471200 Genbank: BX537423|AF016098|BC101525|BC104770|BC117413|BC143238|BC143608| AF022860|AF280545|AF280544|AF022859|AK290934|AF280546|BC009222| AL833606|BX648292|AK130198|BC018631 Entrez Gene: 8828 Refseq: NM_201266|NM_003872|NM_201279|NM_018534|NM_201267|NM_201264 Protein sequence isoform 1 (SEQ ID NO (32)):    1  mdmfpltwvf lalyfsrhqv rgqpdppcgg rlnskdagyi tspgypqdyp shqncewivy   61  apepnqkivl nfnphfeiek hdckydfiei rdgdsesadl lgkhcgniap ptiissgsml  121  yikftsdyar qgagfslrye ifktgsedcs knftspngti espgfpekyp hnldctftil  181  akpkmeiilq flifdlehdp lqvgegdcky dwldiwdgip hvgpligkyc gtktpselrs  241  stgilsltfh tdmavakdgf saryylvhqe plenfqcnvp lgmesgrian eqisasstys  301  dgrwtpqqsr lhgddngwtp nldsnkeylq vdlrfltmlt aiatqgaisr etqngyyvks  361  yklevstnge dwmvyrhgkn hkvfqannda tevvlnklha plltrfvrir pqtwhsgial  421  rlelfgcrvt dapcsnmlgm lsgliadsqi sasstqeylw spsaarlvss rsgwfpripq  481  aqpgeewlqv dlgtpktvkg viiqgarggd sitavearaf vrkfkvsysl ngkdweyiqd  541  prtqqpklfe gnmhydtpdi rrfdpipaqy vrvyperwsp agigmrlevl gcdwtdskpt  601  vetlgptvks eetttpypte eeatecgenc sfeddkdlql psgfncnfdf leepcgwmyd  661  hakwlrttwa sssspndrtf pddrnflrlq sdsqregqya rlisppvhlp rspvcmefqy  721  qatggrgval qvvreasqes kllwviredq ggewkhgrii lpsydmeyqi vfegvigkgr  781  sgeiaiddir istdvplenc mepisafage nfkvdipeih eregyedeid deyevdwsns  841  ssatsgsgap stdkekswly tldpilitii amsslgvllg atcaglllyc tcsysglssr  901  scttlenynf elydglkhkv kmnhqkccse a Protein sequence isoform 2 (SEQ ID NO (33)):    1  mdmfpltwvf lalyfsrhqv rgqpdppcgg rlnskdagyi tspgypqdyp shqncewivy   61  apepnqkivl nfnphfeiek hdckydfiei rdgdsesadl lgkhcgniap ptiissgsml  121  yikftsdyar qgagfslrye ifktgsedcs knftspngti espgfpekyp hnldctftil  181  akpkmeiilq flifdlehdp lqvgegdcky dwldiwdgip hvgpligkyc gtktpselrs  241  stgilsltfh tdmavakdgf saryylvhqe plenfqcnvp lgmesgrian eqisasstys  301  dgrwtpqqsr lhgddngwtp nldsnkeylq vdlrfltmlt aiatqgaisr etqngyyvks  361  yklevstnge dwmvyrhgkn hkvfqannda tevvlnklha plltrfvrir pqtwhsgial  421  rlelfgcrvt dapcsnmlgm lsgliadsqi sasstqeylw spsaarlvss rsgwfpripq  481  aqpgeewlqv dlgtpktvkg viiqgarggd sitavearaf vrkfkvsysl ngkdweyiqd  541  prtqqpklfe gnmhydtpdi rrfdpipaqy vrvyperwsp agigmrlevl gcdwtdskpt  601  vetlgptvks eetttpypte eeatecgenc sfeddkdlql psgfncnfdf leepcgwmyd  661  hakwlrttwa sssspndrtf pddrnflrlq sdsqregqya rlisppvhlp rspvcmefqy  721  qatggrgval qvvreasqes kllwviredq ggewkhgrii lpsydmeyqi vfegvigkgr  781  sgeiaiddir istdvplenc mepisafavd ipeiheregy edeiddeyev dwsnsssats  841  gsgapstdke kswlytldpi litiiamssl gvllgatcag lllyctcsys glssrscttl  901  enynfelydg lkhkvkmnhq kccsea Protein sequence isoform 3 (SEQ ID NO (34)):    1  mdmfpltwvf lalyfsrhqv rgqpdppcgg rlnskdagyi tspgypqdyp shqncewivy   61  apepnqkivl nfnphfeiek hdckydfiei rdgdsesadl lgkhcgniap ptiissgsml  121  yikftsdyar qgagfslrye ifktgsedcs knftspngti espgfpekyp hnldctftil  181  akpkmeiilq flifdlehdp lqvgegdcky dwldiwdgip hvgpligkyc gtktpselrs  241  stgilsltfh tdmavakdgf saryylvhqe plenfqcnvp lgmesgrian eqisasstys  301  dgrwtpqqsr lhgddngwtp nldsnkeylq vdlrfltmlt aiatqgaisr etqngyyvks  361  yklevstnge dwmvyrhgkn hkvfqannda tevvlnklha plltrfvrir pqtwhsgial  421  rlelfgcrvt dapcsnmlgm lsgliadsqi sasstqeylw spsaarlvss rsgwfpripq  481  aqpgeewlqv dlgtpktvkg viiqgarggd sitavearaf vrkfkvsysl ngkdweyiqd  541  prtqqpklfe gnmhydtpdi rrfdpipaqy vrvyperwsp agigmrlevl gcdwtdskpt  601  vetlgptvks eetttpypte eeatecgenc sfeddkdlql psgfncnfdf leepcgwmyd  661  hakwlrttwa sssspndrtf pddrnflrlq sdsqregqya rlisppvhlp rspvcmefqy  721  qatggrgval qvvreasqes kllwviredq ggewkhgrii lpsydmeyqi vfegvigkgr  781  sgeiaiddir istdvplenc mepisafade yevdwsnsss atsgsgapst dkekswlytl  841  dpilitiiam sslgvllgat caglllyctc sysglssrsc ttlenynfel ydglkhkvkm  901  nhqkccsea Protein sequence isoform 4 (SEQ ID NO (35)):    1  mdmfpltwvf lalyfsrhqv rgqpdppcgg rlnskdagyi tspgypqdyp shqncewivy   61  apepnqkivl nfnphfeiek hdckydfiei rdgdsesadl lgkhcgniap ptiissgsml  121  yikftsdyar qgagfslrye ifktgsedcs knftspngti espgfpekyp hnldctftil  181  akpkmeiilq flifdlehdp lqvgegdcky dwldiwdgip hvgpligkyc gtktpselrs  241  stgilsltfh tdmavakdgf saryylvhqe plenfqcnvp lgmesgrian eqisasstys  301  dgrwtpqqsr lhgddngwtp nldsnkeylq vdlrfltmlt aiatqgaisr etqngyyvks  361  yklevstnge dwmvyrhgkn hkvfqannda tevvlnklha plltrfvrir pqtwhsgial  421  rlelfgcrvt dapcsnmlgm lsgliadsqi sasstqeylw spsaarlvss rsgwfpripq  481  aqpgeewlqv dlgtpktvkg viiqgarggd sitavearaf vrkfkvsysl ngkdweyiqd  541  prtqqpklfe gnmhydtpdi rrfdpipaqy vrvyperwsp agigmrlevl gcdwtdskpt  601  vetlgptvks eetttpypte eeatecgenc sfeddkdlql psgfncnfdf leepcgwmyd  661  hakwlrttwa sssspndrtf pddrnflrlq sdsqregqya rlisppvhlp rspvcmefqy  721  qatggrgval qvvreasqes kllwviredq ggewkhgrii lpsydmeyqi vfegvigkgr  781  sgeiaiddir istdvplenc mepisafage nfkggtllpg teptvdtvpm qpipaywyyv  841  maaggavlvl vsvalalvlh yhrfryaakk tdhsitykts hytngaplav eptltikleq  901  drgshc Protein sequence isoform 5 (SEQ ID NO (36)):    1  mdmfpltwvf lalyfsrhqv rgqpdppcgg rlnskdagyi tspgypqdyp shqncewivy   61  apepnqkivl nfnphfeiek hdckydfiei rdgdsesadl lgkhcgniap ptiissgsml  121  yikftsdyar qgagfslrye ifktgsedcs knftspngti espgfpekyp hnldctftil  181  akpkmeiilq flifdlehdp lqvgegdcky dwldiwdgip hvgpligkyc gtktpselrs  241  stgilsltfh tdmavakdgf saryylvhqe plenfqcnvp lgmesgrian eqisasstys  301  dgrwtpqqsr lhgddngwtp nldsnkeylq vdlrfltmlt aiatqgaisr etqngyyvks  361  yklevstnge dwmvyrhgkn hkvfqannda tevvlnklha plltrfvrir pqtwhsgial  421  rlelfgcrvt dapcsnmlgm lsgliadsqi sasstqeylw spsaarlvss rsgwfpripq  481  aqpgeewlqv dlgtpktvkg viiqgarggd sitavearaf vrkfkvsysl ngkdweyiqd  541  prtqqpklfe gnmhydtpdi rrfdpipaqy vrvyperwsp agigmrlevl gcdwtdskpt  601  vetlgptvks eetttpypte eeatecgenc sfeddkdlql psgfncnfdf leepcgwmyd  661  hakwlrttwa sssspndrtf pddrnflrlq sdsqregqya rlisppvhlp rspvcmefqy  721  qatggrgval qvvreasqes kllwviredq ggewkhgrii lpsydmeyqi vfegvigkgr  781  sgeiaiddir istdvplenc mepisafagg tllpgteptv dtvpmqpipa ywyyvmaagg  841  avlvlvsval alvlhyhrfr yaakktdhsi tyktshytng aplaveptlt ikleqdrgsh  901  c Protein sequence isoform 6 (SEQ ID NO (37)):    1  mdmfpltwvf lalyfsrhqv rgqpdppcgg rlnskdagyi tspgypqdyp shqncewivy   61  apepnqkivl nfnphfeiek hdckydfiei rdgdsesadl lgkhcgniap ptiissgsml  121  yikftsdyar qgagfslrye ifktgsedcs knftspngti espgfpekyp hnldctftil  181  akpkmeiilq flifdlehdp lqvgegdcky dwldiwdgip hvgpligkyc gtktpselrs  241  stgilsltfh tdmavakdgf saryylvhqe plenfqcnvp lgmesgrian eqisasstys  301  dgrwtpqqsr lhgddngwtp nldsnkeylq vdlrfltmlt aiatqgaisr etqngyyvks  361  yklevstnge dwmvyrhgkn hkvfqannda tevvlnklha plltrfvrir pqtwhsgial  421  rlelfgcrvt dapcsnmlgm lsgliadsqi sasstqeylw spsaarlvss rsgwfpripq  481  aqpgeewlqv dlgtpktvkg viiqgarggd sitavearaf vrkfkvsysl ngkdweyiqd  541  prtqqpkvgc swrpl Gene ID: X17 Gene symbol: PLXNA2 Gene description: Plexin-A2 Unigene: Hs.497626 Genbank: BC132676 Entrez Gene: 5362 Refseq: NM_025179 Protein sequence (SEQ ID NO (38)):    1  meqrrpwpra levdsrsvvl lsvvwvllap paagmpqfst fhsenrdwtf nhltvhqgtg   61  avyvgainrv ykltgnltiq vahktgpeed nkscyppliv qpcsevltlt nnvnklliid  121  ysenrllacg slyqgvckll rlddlfilve pshkkehyls svnktgtmyg vivrsegedg  181  klfigtavdg kqdyfptlss rklprdpess amldyelhsd fvsslikips dtlalvshfd  241  ifyiygfasg gfvyfltvqp etpegvains agdlfytsri vrlckddpkf hsyvslpfgc  301  tragveyrll qaaylakpgd slaqafnits qddvlfaifs kgqkqyhhpp ddsalcafpi  361  rainlqiker lqscyqgegn lelnwllgkd vqctkapvpi ddnfcgldin qplggstpve  421  gltlyttsrd rmtsvasyvy ngysvvfvgt ksgklkkira dgpphggvqy emvsvlkdgs  481  pilrdmafsi dqrylyvmse rqvtrvpves ceqyttcgec lssgdphcgw calhnmcsrr  541  dkcqqawepn rfaasisqcv slavhpssis vsehsrllsl vvsdapdlsa giacafgnlt  601  evegqvsgsq vicispgpkd vpvipldqdw fglelqlrsk etgkifvste fkfyncsahq  661  lclscvnsaf rchwckyrnl cthdpttcsf qegrinised cpqlvpteei lipvgevkpi  721  tlkarnlpqp qsgqrgyecv lniqgaihrv palrfnsssv qcqnssyqyd gmdisnlavd  781  favvwngnfi idnpqdlkvh lykcaaqres cglclkadrk fecgwcsger rctlhqhcts  841  psspwldwss hnvkcsnpqi teiltvsgpp eggtrvtihg vnlgldfsei ahhvqvagvp  901  ctplpgeyii aeqivcemgh alvgttsgpv rlcigeckpe fmtkshqqyt fvnpsvlsln  961  pirgpesggt mvtitghylg agssvavylg nqtcefygrs mseivcvspp ssnglgpvpv 1021  svsvdrahvd snlqfeyidd prvqriepew siasghtplt itgfnldviq eprirvkfng 1081  kesvnvckvv ntttltclap slttdyrpgl dtverpdefg fvfnnvqsll iyndtkfiyy 1141  pnptfellsp tgvldqkpgs piilkgknlc ppasggakln ytvligetpc avtvsetqll 1201  ceppnltgqh kvmvhvggmv fspgsvsvis dslltlpaiv siaaggslll iiviivliay 1261  krksrendlt lkrlqmqmdn lesrvaleck eafaelqtdi neltsdldrs gipyldyrty 1321  amrvlfpgie dhpvlrelev qgngqqhvek alklfaqlin nkvflltfir tlelqrsfsm 1381  rdrgnvasli mtglqgrley atdvlkqlls dlidknlenk nhpklllrrt esvaekmltn 1441  wfafllhkfl kecageplfm lycaikqqme kgpidaitge aryslsedkl irqqieyktl 1501  ilncvnpdne nspeipvkvl ncdtitqvke kildavyknv pysqrpravd mdlewrqgri 1561  arvvlqdedi ttkiegdwkr lntlmhyqvs drswvalvpk qtssynipas asisrtsisr 1621  ydssfrytgs pdslrsrapm itpdlesgvk vwhlvknhdh gdqkegdrgs kmvseiyltr 1681  llatkgtlqk fvddlfetlf stvhrgsalp laikymfdfl deqadrhsih dtdvrhtwks 1741  nclplrfwvn viknpqfvfd ihkgsitdac lsvvaqtfmd scstsehrlg kdspsnklly 1801  akdipsyksw veryyadiak lpaisdqdmn aylaeqsrlh avefnmlsal neiysyvsky 1861  seeligaleq deqarrqrla ykveqlinam sies Gene ID: X18 Gene symbol: PCDHA4 Gene description: Protocadherin alpha-4 Unigene: Hs.199343 Genbank: AF152482|AF152312 Entrez Gene: 56144 Refseq: NM_018907|NM_031500 Protein sequence isoform 1 (SEQ ID NO (39)):    1  mefswgsgqe srrlllllll laaweagngq lhysvseeak hgtfvgriaq dlglelaelv   61  prlfrvaskg rggllevnlq ngilfvnsri dreelcrrsa ecsihleviv drplqvfhvd  121  vevrdindnp pvfpatqknl siaesrplds rfplegasda digenallty rlspneyfsl  181  ekppddelvk glglilrksl dreeapeifl vltatdggkp eltgtvqlli tvldandnap  241  afdrtiykvr llenvpngtl viklnasdld eglngdivys fsndispnvk skfhidpitg  301  qiivkgyidf eesksyeiiv egidkgqlpl sghcrvivev ednndnvpdl efkslslpir  361  edaplgtvia lisvsdkdmg vnglvtcslt shvpfklvst fknyyslvld saldresvsa  421  yelvvtardg gspslwatas vsvevadvnd napafaqpey tvfvkennpp gchiftvsaw  481  dadaqenalv syslverrvg eralssyvsv haesgkvyal qpldheelel lqfqvtarda  541  gvpplgsnvt lqvfvldend napallapra ggtggavsel vpwsvgvghv vakvravdad  601  sgynawlsye lqpgtggari pfrvglytge isttraldet daprhrllvl vkdhgepalt  661  atatvlvslv esgqapkass ralvgavgpd aalvdvnvyl iiaicavssl lvltlllyta  721  lrcsalpteg acapgkptlv cssavgswsy sqqrrprvcs gegppktdlm afspslpdsr  781  dredqlqtte esfakprqpn pdwrysaslr agmhssvhle eagilragpg gpdqqwptvs  841  satpepeage vsppvgagvn snswtfkygp gnpkqsgpge lpdkfiipgs paiisirqep  901  tnsqidksdf itfgkkeetk kkkkkkkgnk tqekkekgns ttdnsdq Protein sequence isoform 2 (SEQ ID NO (40)):    1  mefswgsgqe srrlllllll laaweagngq lhysvseeak hgtfvgriaq dlglelaelv   61  prlfrvaskg rggllevnlq ngilfvnsri dreelcrrsa ecsihleviv drplqvfhvd  121  vevrdindnp pvfpatqknl siaesrplds rfplegasda digenallty rlspneyfsl  181  ekppddelvk glglilrksl dreeapeifl vltatdggkp eltgtvqlli tvldandnap  241  afdrtiykvr llenvpngtl viklnasdld eglngdivys fsndispnvk skfhidpitg  301  qiivkgyidf eesksyeiiv egidkgqlpl sghcrvivev ednndnvpdl efkslslpir  361  edaplgtvia lisvsdkdmg vnglvtcslt shvpfklvst fknyyslvld saldresvsa  421  yelvvtardg gspslwatas vsvevadvnd napafaqpey tvfvkennpp gchiftvsaw  481  dadaqenalv syslverrvg eralssyvsv haesgkvyal qpldheelel lqfqvtarda  541  gvpplgsnvt lqvfvldend napallapra ggtggavsel vpwsvgvghv vakvravdad  601  sgynawlsye lqpgtggari pfrvglytge isttraldet daprhrllvl vkdhgepalt  661  atatvlvslv esgqapkass ralvgavgpd aalvdvnvyl iiaicavssl lvltlllyta  721  lrcsalpteg acapgkptlv cssavgswsy sqqrrprvcs gegppktdlm afspslpdsr  781  dredqlqtte esfakvsv Gene ID: X19 Gene symbol: PCDHAC2 Gene description: Protocadherin alpha-C2 Unigene: Hs.199343 Genbank: AF152304 Entrez Gene: 56134 Refseq: NM_018899|NM_031883 Protein sequence isoform 1 (SEQ ID NO (41)):    1  meqagtrpaa tehprlrrpm pwllllplll llllllpgpa asqlrysvpe eqapgalvgn   61  varalglelr rlgpgclrin hlgapspryl eldltsgalf vneridreal ceqrprclls  121  levlahnpva vsaveveild indnsprfpr pnyqlqvses vapgarfhie saqdpdvgan  181  svqtyelsps ehfeldlkpl qenskvlelv lrkgldreqa alhhlvltav dggiparsgt  241  aqisvrvldt ndnspafdqs tyrvqlreds ppgtlvvkln asdpdegsng elryslssyt  301  sdrerqlfsi dastgevrvi ggldyeeass yqiyvqatdr gpvpmaghck vlvdivdvnd  361  napevvltdl yspvpenatp ntivavlsvn dqdsgpnrkv slgleatlpf rlngfgnsyt  421  lvvsgpldre rvavynitvt atdggipqlt slrtlkveis dindnppsfl edsysiyiqe  481  nnlpgvllct vqatdpdeke naevtyslle reiqglpvts yvsinsasgs lyavnsfdye  541  kfreffvtve aqdkgsppls stvtanvyvv dmndhaphil yptstnssaa femvprtapa  601  gylvtkviam dsdsgqnawl fyhlaqtsdl dlfkvelhtg eirttrkmgd esgstfnltv  661  vvrdngepsl sasvaitvav vdrvskilpd tqrhvksprt yseitlylii alstvsfifl  721  ltiiilsiik cyrytaygta ccggfcgvre rspaelykqa nnnidariph glkvqphfie  781  vrgngsltkt ycykacltag sgsdtfmfyn tgaqtgpgps gaqaavtdsr nltgqsgqna  841  gnliilknea vsqneprqpn pdwrysaslr agmhssvhle eagilragpg gpdqqwptvs  901  satpepeage vsppvgagvn snswtfkygp gnpkqsgpge lpdkfiipgs paiisirqep  961  tnsqidksdf itfgkkeetk kkkkkkkgnk tqekkekgns ttdnsdq Protein sequence isoform 2 (SEQ ID NO (42)):    1  meqagtrpaa tehprlrrpm pwllllplll llllllpgpa asqlrysvpe eqapgalvgn   61  varalglelr rlgpgclrin hlgapspryl eldltsgalf vneridreal ceqrprclls  121  levlahnpva vsaveveild indnsprfpr pnyqlqvses vapgarfhie saqdpdvgan  181  svqtyelsps ehfeldlkpl qenskvlelv lrkgldreqa alhhlvltav dggiparsgt  241  aqisvrvldt ndnspafdqs tyrvqlreds ppgtlvvkln asdpdegsng elryslssyt  301  sdrerqlfsi dastgevrvi ggldyeeass yqiyvqatdr gpvpmaghck vlvdivdvnd  361  napevvltdl yspvpenatp ntivavlsvn dqdsgpnrkv slgleatlpf rlngfgnsyt  421  lvvsgpldre rvavynitvt atdggipqlt slrtlkveis dindnppsfl edsysiyiqe  481  nnlpgvllct vqatdpdeke naevtyslle reiqglpvts yvsinsasgs lyavnsfdye  541  kfreffvtve aqdkgsppls stvtanvyvv dmndhaphil yptstnssaa femvprtapa  601  gylvtkviam dsdsgqnawl fyhlaqtsdl dlfkvelhtg eirttrkmgd esgstfnltv  661  vvrdngepsl sasvaitvav vdrvskilpd tqrhvksprt yseitlylii alstvsfifl  721  ltiiilsiik cyrytaygta ccggfcgvre rspaelykqa nnnidariph glkvqphfie  781  vrgngsltkt ycykacltag sgsdtfmfyn tgaqtgpgps gaqaavtdsr nltgqsgqna  841  gnliilknea vsqnevrqws ggllqthafv thppiscdla llsh Gene ID: X20 Gene symbol: GPC4 Gene description: Glypican 4 Unigene: Hs.58367 Genbank: AF030186 Entrez Gene: 2239 Refseq: NM_001448 Protein sequence (SEQ ID NO (43)):    1  mrllwklvil lplinssagd gllsrpiftq ephdvifpld lsksevilnc aangypsphy   61  rwkqngtdid ftmsyhyrld ggslainsph tdqdigmyqc latnllgtil srkaklqfay  121  iedfetktrs tvsvregqgv vllcgppphf gdlsyawtfn dnplyvqedn rrfvsqetgn  181  lyiakvepsd vgnytcfitn keaqrsvqgp ptplvqrtdg vmgeyepkie vrfpetiqaa  241  kdssvklecf algnpvpdis wrrldgsplp gkvkysksqa ileipnfqqe degfyecias  301  nlrgrnlakg qlifyappew eqkiqnthls iydnllweck asgkpnpwyt wlkngerlnp  361  eeriqiengt liitmlnvsd sgvyqcaaen kyqiiyanae lrvlasapdf skspvkkksf  421  vqvggdivig ckpnafpraa iswkrgtetl rqskriflle dgslkiynit rsdagsytci  481  atnqfgtakn tgslivkert vitvppskmd vtvgesivlp cqvshdpsie vvfvwffngd  541  vidlkkgvah feriggesvg dlmirniqlh hsgkylctvq ttleslsava diivrgppgp  601  pedvqvedis sttsqlswra gpdnnspiqi ftiqtrtpfs vgwqavatvp eilngktyna  661  tvvglspwve yefrvvagns igigepseps ellrtkasvp vvapvnihgg ggsrselvit  721  wesipeelqn gegfgyiimf rpvgsttwsk ekvssvessr fvyrnesiip lspfevkvgv  781  ynnegegsls tvtivysged epqlaprgts lqsfsaseme vswnaiawnr ntgrvlgyev  841  lywtddskes migkirvsgn vttknitglk antiyfasvr ayntagtgps sppvnvttkk  901  sppsqppani awkltnsklc lnwehvktme nesevlgyki lyrqnrqskt hiletnntsa  961  ellvpfeedy lieirtvsdg gdgssseeir ipkmsslssr giqflepsth flsivivifh 1021  cfaiqpli Gene ID: X21 Gene symbol: CNTN6 Gene description: Contactin 6 Unigene: Hs.387300 Genbank: AB003592 Entrez Gene: 27255 Refseq: NM_014461 Protein sequence (SEQ ID NO (44)):    1  marfglpall ctlavlsaal laaelksksc sevrrlyvsk gfnkndaplh eingdhlkic   61  pqgstccsqe meekyslqsk ddfksvvseq cnhlqavfas rykkfdeffk ellenaeksl  121  ndmfvktygh lymqnselfk dlfvelkryy vvgnvnleem lndfwarlle rmfrivnsqy  181  hftdeylecv skyteqlkpf gdvprklklq vtrafvaart faqglavagd vvskvsvvnp  241  taqcthallk miycshcrgl vtvkpcynyc snimrgclan qgdldfewnn fidamlmvae  301  rlegpfnies vmdpidvkis daimnmqdns vqvsqkvfqg cgppkplpag risrsisesa  361  fsarfrphhp eerpttaagt sldrlvtdvk eklkqakkfw sslpsnvcnd ermaagngne  421  ddcwngkgks rylfavtgng lanqgnnpev qvdtskpdil ilrqimalrv mtskmknayn  481  gndvdffdis dessgegsgs gceyqqcpse fdynatdhag ksanekadsa gvrpgaqayl  541  ltvfcilflv mqrewr Gene ID: X22 Gene symbol: SLC9A7 Gene description: solute carrier family 9 (sodium/hydrogen exchanger), member 7 Unigene: Hs.496057 Genbank: AF298591 Entrez Gene: 84679 Refseq: NM_001257291|NM_032591 Protein sequence isoform 1 (SEQ ID NO (45)):    1  mepgdaarpg sgratgappp rllllplllg wglrvaaaas asssgaaaed ssameelate   61  keaeeshrqd svslltfill ltltiltiwl fkhrrvrflh etglamiygl ivgvilrygt  121  patsgrdksl sctqedrafs tllvnvsgkf feytlkgeis pgkinsveqn dmlrkvtfdp  181  evffnillpp iifhagyslk krhffrnlgs ilayaflgta vscfiignlm ygvvklmkim  241  gqlsdkfyyt dclffgaiis atdpvtvlai fnelhadvdl yallfgesvl ndavaivlss  301  sivayqpagl nthafdaaaf fksvgiflgi fsgsftmgav tgvvtalvtk ftklhcfpll  361  etalfflmsw stfllaeacg ftgvvavlfc gitqahytyn nlsvesrsrt kqlfevlhfl  421  aenfifsymg lalftfqkhv fspifiigaf vaiflgraah iyplsfflnl grrhkigwnf  481  qhmmmfsglr gamafalair dtasyarqmm ftttllivff tvwiigggtt pmlswlnirv  541  gveepseedq nehhwqyfrv gvdpdqdppp nndsfqvlqg dgpdsargnr tkqesawifr  601  lwysfdhnyl kpilthsgpp ltttlpawcg llarcltspq vydnqeplre edsdfilteg  661  dltltygdst vtangssssh tastslegsr rtkssseevl erdlgmgdqk vssrgtrlvf  721  pledna Protein sequence isoform 2 (SEQ ID NO (46)):    1  mepgdaarpg sgratgappp rllllplllg wglrvaaaas asssgaaaed ssameelate   61  keaeeshrqd svslltfill ltltiltiwl fkhrrvrflh etglamiygl ivgvilrygt  121  patsgrdksl sctqedrafs tllvnvsgkf feytlkgeis pgkinsveqn dmlrkvtfdp  181  evffnillpp iifhagyslk krhffrnlgs ilayaflgta vscfiignlm ygvvklmkim  241  gqlsdkfyyt dclffgaiis atdpvtvlai fnelhadvdl yallfgesvl ndavaivlss  301  sivayqpagl nthafdaaaf fksvgiflgi fsgsftmgav tgvnanvtkf tklhcfplle  361  talfflmsws tfllaeacgf tgvvavlfcg itqahytynn lsvesrsrtk qlfevlhfla  421  enfifsymgl alftfqkhvf spifiigafv aiflgraahi yplsfflnlg rrhkigwnfq  481  hmmmfsglrg amafalaird tasyarqmmf tttllivfft vwiigggttp mlswlnirvg  541  veepseedqn ehhwqyfrvg vdpdqdpppn ndsfqvlqgd gpdsargnrt kqesawifrl  601  wysfdhnylk pilthsgppl tttlpawcgl larcltspqv ydnqeplree dsdfiltegd  661  ltltygdstv tangssssht astslegsrr tkssseevle rdlgmgdqkv ssrgtrlvfp  721  ledna Gene ID: X23 Gene symbol: PVRL3 Gene description: poliovirus receptor-related 3 Unigene: Hs.293917 Genbank: AK075105 Entrez Gene: 25945 Refseq: NM_015480|NM_001243286|NM_001243288 Protein sequence isoform 1 (SEQ ID NO (47)):    1  martlrpspl cpgggkaqls sasllgagll lqpptpppll lllfplllfs rlcgalagpi   61  ivephvtavw gknvslkcli evnetitqis wekihgkssq tvavhhpqyg fsvqgeyqgr  121  vlfknyslnd atitlhnigf sdsgkyicka vtfplgnaqs sttvtvlvep tvslikgpds  181  lidggnetva aiciaatgkp vahidwegdl gemestttsf pnetatiisq yklfptrfar  241  grritcvvkh palekdirys fildiqyape vsvtgydgnw fvgrkgvnlk cnadanpppf  301  ksvwsrldgq wpdgllasdn tlhfvhpltf nysgvyickv tnslgqrsdq kviyisdppt  361  tttlqptiqw hpstadiedl atepkklpfp lstlatikdd tiatiiasvv ggalfivlvs  421  vlagifcyrr rrtfrgdyfa knyippsdmq kesqidvlqq deldsypdsv kkenknpvnn  481  lirkdyleep ektqwnnven lnrferpmdy yedlkmgmkf vsdehydene ddlvshvdgs  541  visrrewyv Protein sequence isoform 2 (SEQ ID NO (48)):    1  martlrpspl cpgggkaqls sasllgagll lqpptpppll lllfplllfs rlcgalagpi   61  ivephvtavw gknvslkcli evnetitqis wekihgkssq tvavhhpqyg fsvqgeyqgr  121  vlfknyslnd atitlhnigf sdsgkyicka vtfplgnaqs sttvtvlvep tvslikgpds  181  lidggnetva aiciaatgkp vahidwegdl gemestttsf pnetatiisq yklfptrfar  241  grritcvvkh palekdirys fildiqyape vsvtgydgnw fvgrkgvnlk cnadanpppf  301  ksvwsrldgq wpdgllasdn tlhfvhpltf nysgvyickv tnslgqrsdq kviyisayns  361  vaslnc Protein sequence isoform 3 (SEQ ID NO (49)):    1  maegwrwcfv rrtpgllrgp llprsfsgnp ralagpiive phvtavwgkn vslkclievn   61  etitqiswek ihgkssqtva vhhpqygfsv qgeyqgrvlf knyslndati tlhnigfsds  121  gkyickavtf plgnaqsstt vtvlveptvs likgpdslid ggnetvaaic iaatgkpvah  181  idwegdlgem estttsfpne tatiisqykl fptrfargrr itcvvkhpal ekdirysfil  241  diqyapevsv tgydgnwfvg rkgvnlkcna danpppfksv wsrldgqwpd gllasdntlh  301  fvhpltfnys gvyickvtns lgqrsdqkvi yisdvpfkqt ssiavagavi gavlalfiia  361  ifvtvlltpr kkrpsyldkv idlppthkpp plyeersppl pqkdlfqpeh lplqtqfker  421  evgnlqhsng lnsrsfdyed enpvgedgiq qmyplynqmc yqdrspgkhh qnndpkrvyi  481  dprehyv Gene ID: X24 Gene symbol: SLC4A4 Gene description: solute carrier family 4, sodium bicarbonate cotransporter, member 4 Unigene: Hs.5462 Genbank: AF011390 Entrez Gene: 8671 Refseq: NM_001098484|NM_001134742|NM_003759 Protein sequence isoform 1 (SEQ ID NO (50)):    1  medeavldrg asflkhvcde eeveghhtiy igvhvpksyr rrrrhkrktg hkekkekeri   61  senysdksdi enadessssi lkplispaae rirfilgeed dspappqlft eldellavdg  121  qemewketar wikfeekveq ggerwskphv atlslhslfe lrtcmekgsi mldreasslp  181  qlvemivdhq ietgllkpel kdkvtytllr khrhqtkksn lrsladigkt vssasrmftn  241  pdngspamth rnltssslnd isdkpekdql knkfmkklpr daeasnvlvg evdfldtpfi  301  afvrlqqavm lgaltevpvp trflfillgp kgkaksyhei graiatlmsd evfhdiayka  361  kdrhdliagi defldevivl ppgewdpair ieppkslpss dkrknmysgg envqmngdtp  421  hdgghggggh gdceelqrtg rfcgglikdi krkapffasd fydalniqal sailfiylat  481  vtnaitfggl lgdatdnmqg vlesflgtav sgaifclfag qpltilsstg pvlvferllf  541  nfskdnnfdy lefrlwiglw saflclilva tdasflvqyf trfteegfss lisfifiyda  601  fkkmiklady ypinsnfkvg yntlfsctcv ppdpanisis ndttlapeyl ptmsstdmyh  661  nttfdwafls kkecskyggn lvgnncnfvp ditlmsfilf lgtytssmal kkfktspyfp  721  ttarklisdf aiilsilifc vidalvgvdt pklivpsefk ptspnrgwfv ppfgenpwwv  781  claaaipall vtilifmdqq itavivnrke hklkkgagyh ldlfwvailm vicslmalpw  841  yvaatvisia hidslkmete tsapgeqpkf lgvreqrvtg tlvfiltgls vfmapilkfi  901  pmpvlygvfl ymgvasingv qfmdrlklll mplkhqpdfi ylrhvplrrv hlftflqvlc  961  lallwilkst vaaiifpvmi lalvavrkgm dylfsqhdls flddvipekd kkkkedekkk 1021  kkkkgsldsd nddsdcpyse kvpsikipmd imeqqpflsd skpsdrersp tflerhtsc Protein sequence isoform 3 (SEQ ID NO (51)):    1  medeavldrg asflkhvcde eeveghhtiy igvhvpksyr rrrrhkrktg hkekkekeri   61  senysdksdi enadessssi lkplispaae rirfilgeed dspappqlft eldellavdg  121  qemewketar wikfeekveq ggerwskphv atlslhslfe lrtcmekgsi mldreasslp  181  qlvemivdhq ietgllkpel kdkvtytllr khrhqtkksn lrsladigkt vssasrmftn  241  pdngspamth rnltssslnd isdkpekdql knkfmkklpr daeasnvlvg evdfldtpfi  301  afvrlqqavm lgaltevpvp trflfillgp kgkaksyhei graiatlmsd evfhdiayka  361  kdrhdliagi defldevivl ppgewdpair ieppkslpss dkrknmysgg envqmngdtp  421  hdgghggggh gdceelqrtg rfcgglikdi krkapffasd fydalniqal sailfiylat  481  vtnaitfggl lgdatdnmqg vlesflgtav sgaifclfag qpltilsstg pvlvferllf  541  nfskdnnfdy lefrlwiglw saflclilva tdasflvqyf trfteegfss lisfifiyda  601  fkkmiklady ypinsnfkvg yntlfsctcv ppdpanisis ndttlapeyl ptmsstdmyh  661  nttfdwafls kkecskyggn lvgnncnfvp ditlmsfilf lgtytssmal kkfktspyfp  721  ttarklisdf aiilsilifc vidalvgvdt pklivpsefk ptspnrgwfv ppfgenpwwv  781  claaaipall vtilifmdqq itavivnrke hklkkgagyh ldlfwvailm vicslmalpw  841  yvaatvisia hidslkmete tsapgeqpkf lgvreqrvtg tlvfiltgls vfmapilkfi  901  pmpvlygvfl ymgvaslngv qfmdrlklll mplkhqpdfi ylrhvplrrv hlftflqvlc  961  lallwilkst vaaiifpvmi lalvavrkgm dylfsqhdls flddvipekd kkkkedekkk 1021  kkkkgsldsd nddekdhqhs lnathhadki pflqslgmps pprtpvkvvp qirieleped 1081  ndyfwrskgt ettl Protein sequence isoform 2 (SEQ ID NO (52)):    1  mstenvegkp snlgergrar sstflrvvqp mfnhsiftsa vspaaerirf ilgeeddspa   61  ppqlftelde llavdgqeme wketarwikf eekveqgger wskphvatls lhslfelrtc  121  mekgsimldr easslpqlve mivdhqietg llkpelkdkv tytllrkhrh qtkksnlrsl  181  adigktvssa srmftnpdng spamthrnlt ssslndisdk pekdqlknkf mkklprdaea  241  snvlvgevdf ldtpfiafvr lqqavmlgal tevpvptrfl fillgpkgka ksyheigrai  301  atlmsdevfh diaykakdrh dliagidefl devivlppge wdpairiepp kslpssdkrk  361  nmysggenvq mngdtphdgg hgggghgdce elqrtgrfcg glikdikrka pffasdfyda  421  lniqalsail fiylatvtna itfggllgda tdnmqgvles flgtavsgai fclfagqplt  481  ilsstgpvlv ferllfnfsk dnnfdylefr lwiglwsafl clilvatdas flvqyftrft  541  eegfsslisf ifiydafkkm ikladyypin snfkvgyntl fsctcvppdp anisisndtt  601  lapeylptms stdmyhnttf dwaflskkec skyggnlvgn ncnfvpditl msfilflgty  661  tssmalkkfk tspyfpttar klisdfaiil silifcvida lvgvdtpkli vpsefkptsp  721  nrgwfvppfg enpwwvclaa aipallvtil ifmdqqitav ivnrkehklk kgagyhldlf  781  wvailmvics lmalpwyvaa tvisiahids lkmetetsap geqpkflgvr eqrvtgtlvf  841  iltglsvfma pilkfipmpv lygvflymgv aslngvqfmd rlklllmplk hqpdfiylrh  901  vplrrvhlft flqvlclall wilkstvaai ifpvmilalv avrkgmdylf sqhdlsfldd  961  vipekdkkkk edekkkkkkk gsldsdndds dcpysekvps ikipmdimeq qpflsdskps 1021  drersptfle rhtsc Gene ID: X25 Gene symbol: CXADR Gene description: coxsackie virus and adenovirus receptor Unigene: Hs.634837 Genbank: AY072912|AY072911|AY072910|AK313526| Entrez Gene: 1525 Refseq: NM_001338|NM_001207063|NM_001207064|NM_001207065| NM_001207066 Protein sequence isoform 1 (SEQ ID NO (53)):    1  malllcfvll cgvvdfarsl sittpeemie kakgetaylp ckftlspedq gpldiewlis   61  padnqkvdqv iilysgdkiy ddyypdlkgr vhftsndlks gdasinvtnl qlsdigtyqc  121  kvkkapgvan kkihlvvlvk psgarcyvdg seeigsdfki kcepkegslp lqyewqklsd  181  sqkmptswla emtssvisvk nasseysgty sctvrnrvgs dqcllrlnvv ppsnkaglia  241  gaiigtllal aligliifcc rkkrreekye kevhhdired vpppksrtst arsyigsnhs  301  slgsmspsnm egysktqynq vpsedfertp qsptlppakv aapnlsrmga ipvmipaqsk  361  dgsiv Protein sequence isoform 2 (SEQ ID NO (54)):    1  malllcfvll cgvvdfarsl sittpeemie kakgetaylp ckftlspedq gpldiewlis   61  padnqkvdqv iilysgdkiy ddyypdlkgr vhftsndlks gdasinvtnl qlsdigtyqc  121  kvkkapgvan kkihlvvlvk psgarcyvdg seeigsdfki kcepkegslp lqyewqklsd  181  sqkmptswla gkmchlqrav rplpeatsav iihpwgpcll ptwkdiprls itkyqvktln  241  allrvrlshl lr Protein sequence isoform 3 (SEQ ID NO (55)):    1  malllcfvll cgvvdfarsl sittpeemie kakgetaylp ckftlspedq gpldiewlis   61  padnqkvdqv iilysgdkiy ddyypdlkgr vhftsndlks gdasinvtnl qlsdigtyqc  121  kvkkapgvan kkihlvvlgk mchlqravrp lpeatsavii hpwgpcllpt wkdiprlsit  181  kyqvktlnal lrvrlshllr Protein sequence isoform 4 (SEQ ID NO (56)):    1  malllcfvll cgvvdfarsl sittpeemie kakgetaylp ckftlspedq gpldiewlis   61  padnqkvdqv grcatskepy vhcqklhrq Protein sequence isoform 5 (SEQ ID NO (57)):    1  malllcfvll cgvvdfarsl sittpeemie kakgetaylp ckftlspedq gpldiewlis   61  padnqkvdqv iilysgdkiy ddyypdlkgr vhftsndlks gdasinvtnl qlsdigtyqc  121  kvkkapgvan kkihlvvlvk psgarcyvdg seeigsdfki kcepkegslp lqyewqklsd  181  sqkmptswla emtssvisvk nasseysgty sctvrnrvgs dqcllrlnvv ppsnkaglia  241  gaiigtllal aligliifcc rkkrreekye kevhhdired vpppksrtst arsyigsnhs  301  slgsmspsnm egysktqynq vpsedfertp qsptlppakf kypyktdgit vv Gene ID: X26 Gene symbol: CADM4 Gene description: cell adhesion molecule 4 Unigene: Hs.370984 Genbank: AF363368 Entrez Gene: 199731 Refseq: NM_145296 Protein sequence (SEQ ID NO (58)):    1  mgrarrfqwp llllwaaaag pgagqevqte nvtvaeggva eitcrlhqyd gsivviqnpa   61  rqtlffngtr alkderfqle efsprrvrir lsdarledeg gyfcqlyted thhqiatltv  121  lvapenpvve vreqavegge velsclvprs rpaatlrwyr drkelkgvss sqengkvwsv  181  astvrfrvdr kddggiiice aqnqalpsgh skqtqyvldv qysptariha sqavvregdt  241  lvltcavtgn prpnqirwnr gneslperae avgetltlpg lvsadngtyt ceasnkhgha  301  ralyvlvvyd pgavveaqts vpyaivggil allvfliicv lvgmvwcsvr qkgsylthea  361  sgldeqgear eaflngsdgh krkeeffi Gene ID: Y1 Gene symbol: CLCA2 Gene description: chloride channel accessory 2 Unigene: Hs.241551 Genbank: BC041096 Entrez Gene: 9635 Refseq: NM_006536 Protein sequence (SEQ ID NO (59)):    1  mtqrsiagpi cnlkfvtllv alsselpflg agvqlqdngy nglliainpq vpenqnlisn   61  ikemiteasf ylfnatkrrv ffrnikilip atwkannnsk ikqesyekan vivtdwygah  121  gddpytlqyr gcgkegkyih ftpnfllndn ltagygsrgr vfvhewahlr wgvfdeynnd  181  kpfyingqnq ikvtressdi tgifvcekgp cpqenciisk lfkegctfiy nstqnatasi  241  mfmqslssvv efcnasthnq eapnlqnqmc slrsawdvit dsadfhhsfp mngtelpppp  301  tfslvqagdk wvclvldvss kmaeadrllq lqqaaefylm qiveihtfvg iasfdskgei  361  raqlhqinsn ddrkllvsyl pttvsaktdi sicsglkkgf evveklngka ygsvmilvts  421  gddkllgncl ptvlssgsti hsialgssaa pnleelsrlt gglkffvpdi snsnsmidaf  481  srissgtgdi fqqhiqlest genvkphhql kntvtvdntv gndtmflvtw qasgppeiil  541  fdpdgrkyyt nnfitnltfr taslwipgta kpghwtytln nthhslqalk vtvtsrasns  601  avppatveaf verdslhfph pvmiyanvkq gfypilnatv tatvepetgd pvtlrllddg  661  agadvikndg iysryffsfa angryslkvh vnhspsistp ahsipgsham yvpgytangn  721  iqmnaprksv grneeerkwg fsrvssggsf svlgvpagph pdvfppckii dleavkveee  781  ltlswtapge dfdqgqatsy eirmskslqn iqddfnnail vntskrnpqq agireiftfs  841  pqistngpeh qpngethesh riyvairamd rnslqsavsn iaqaplfipp nsdpvpardy  901  lilkgvltam gligiiclii vvthhtlsrk kradkkengt kll Gene ID: Y2 Gene symbol: ECM1 Gene description: extracellular matrix protein 1 Unigene: Hs.81071 Genbank: U68187|U68186|AK097046 Entrez Gene: 1893 Refseq: NM_004425|NM_022664|NM_001202858 Protein sequence isoform 1 (SEQ ID NO (60)):    1  mgttaraalv ltylavasaa seggftatgq rqlrpehfqe vgyaappspp lsrslpmdhp   61  dssqhgppfe gqsqvqppps qeatplqqek llpaqlpaek evgpplpqea vplqkelpsl  121  qhpneqkegt papfgdqshp epeswnaaqh cqqdrsqggw ghrldgfppg rpspdnlnqi  181  clpnrqhvvy gpwnlpqssy shltrqgetl nfleigysrc chershtnrl ecaklvweea  241  msrfceaefs vktrphwcct rqgearfscf qeeapqphyq lracpshqpd issglelpfp  301  pgvptldnik nichlrrfrs vprnlpatdp lqrellaliq lerefqrcer qgnnhtctwk  361  awedtldkyc dreyavkthh hlccrhppsp trdecfarra pypnydrdil tidigrvtpn  421  lmghlcgnqr vltkhkhipg lihnmtarcc dlpfpeqacc aeeekltfin dlcgprrniw  481  rdpalccyls pgdeqvncfn inylrnvalv sgdtenakgq geqgstggtn isstsepkee Protein sequence isoform 2 (SEQ ID NO (61)):    1  mgttaraalv ltylavasaa seggftatgq rqlrpehfqe vgyaappspp lsrslpmdhp   61  dssqhgppfe gqsqvqppps qeatplqqek llpaqlpaek evgpplpqea vplqkelpsl  121  qhpneqkegt papfgdqshp epeswnaaqh cqqdrsqggw ghrldgfppg rpspdnlnqi  181  clpnrqhvvy gpwnlpqssy shltrqgetl nfleigysre chershtnrl ecaklvwedt  241  ldkycdreya vkthhhlccr hppsptrdec farrapypny drdiltidig rvtpnlmghl  301  cgnqrvltkh khipglihnm tarccdlpfp eqaccaeeek ltfindlcgp rrniwrdpal  361  ccylspgdeq vncfninylr nvalvsgdte nakgqgeqgs tggtnissts epkee Protein sequence isoform 3 (SEQ ID NO (62)):    1  mgttaraalv ltylavasaa seggftatgq rqlrpehfqe vgyaappspp lsrslpmdhp   61  dssqhgppfe gqsgkegrgp rphsqpwlge rvgcshipps ivqpppsqea tplqqekllp  121  aqlpaekevg pplpqeavpl qkelpslqhp neqkegtpap fgdqshpepe swnaaqhcqq  181  drsqggwghr ldgfppgrps pdnlnqiclp nrqhvvygpw nlpqssyshl trqgetlnfl  241  eigysrcchc rshtnrleca klvweeamsr fceaefsvkt rphwcctrqg earfscfqee  301  apqphyqlra cpshqpdiss glelpfppgv ptldniknic hlrrfrsvpr nlpatdplqr  361  ellaliqler efqrccrqgn nhtctwkawe dtldkycdre yavkthhhlc crhppsptrd  421  ecfarrapyp nydrdiltid igrvtpnlmg hlcgnqrvlt khkhipglih nmtarccdlp  481  fpeqaccaee ekltfindlc gprrniwrdp alccylspgd eqvncfniny lrnvalvsgd  541  tenakgqgeq gstggtniss tsepkee Gene ID: Y3 Gene symbol: CLDN1 Gene description: claudin 1 Unigene: Hs.439060 Genbank: AY358652 Entrez Gene: 9076 Refseq: NM_021101 Protein sequence (SEQ ID NO (63)):    1  managlqllg filaflgwig aivstalpqw riysyagdni vtaqamyegl wmscvsqstg   61  qiqckvfdsl lnlsstlqat ralmvvgill gviaifvatv gmkcmkcled devqkmrmav  121  iggaifllag lailvatawy gnrivqefyd pmtpvnarye fgqalftgwa aaslcllgga  181  llccscprkt tsyptprpyp kpapssgkdy v Gene ID: Y4 Gene symbol: SFN Gene description: stratifin Unigene: Hs.523718 Genbank: AF029082 Entrez Gene: 2810 Refseq: NM_006142 Protein sequence (SEQ ID NO (64)):    1  merasliqka klaeqaerye dmaafmkgav ekgeelscee rnllsvaykn vvggqraawr   61  vlssieqksn eegseekgpe vreyrekvet elqgvcdtvl glldshlike agdaesrvfy  121  lkmkgdyyry laevatgddk kriidsarsa yqeamdiskk empptnpirl glalnfsvfh  181  yeianspeea islakttfde amadlhtlse dsykdstlim qllrdnltlw tadnageegg  241  eapqepqs Gene ID: Y5 Gene symbol: CD9 Gene description: CD9 antigen Unigene: Hs.114286 Genbank: AY966455 Entrez Gene: 928 Refseq: NM_001769 Protein sequence (SEQ ID NO (65)):    1  mpvkggtkci kyllfgfnfi fwlagiavla iglwlrfdsq tksifeqetn nnnssfytgv   61  yiligagalm mlvgflgccg avqesqcmlg lffgfllvif aieiaaaiwg yshkdevike  121  vqefykdtyn klktkdepqr etlkaihyal nccglaggve qfisdicpkk dvletftvks  181  cpdaikevfd nkfhiigavg igiavvmifg mifsmilcca irmremv Gene ID: Y6 Gene symbol: CD109 Gene description: CD109 antigen Unigene: Hs.399891 Genbank: AF410459 Entrez Gene: 135228 Refseq: NM_133493|NM_001159587|NM_001159588 Protein sequence isoform 1 (SEQ ID NO (66)):    1  mqgpplltaa hllcvctaal avapgprflv tapgiirpgg nvtigvelle hcpsqvtvka   61  ellktasnlt vsvleaegvf ekgsfktltl pslplnsade iyelrvtgrt qdeilfsnst  121  rlsfetkris vfiqtdkaly kpkqevkfri vtlfsdfkpy ktslnilikd pksnliqqwl  181  sqqsdlgvis ktfqlsshpi lgdwsiqvqv ndqtyyqsfq vseyvlpkfe vtlqtplycs  241  mnskhlngti takytygkpv kgdvtltflp lsfwgkkkni tktfkingsa nfsfndeemk  301  nvmdssngls eyldlsspgp veilttvtes vtgisrnvst nvffkqhdyi ieffdyttvl  361  kpslnftatv kvtradgnql tleerrnnvv itvtqrnyte ywsgsnsgnq kmeavqkiny  421  tvpqsgtfki efpiledsse lqlkayflgs kssmavhslf kspsktyiql ktrdenikvg  481  spfelvvsgn krlkelsymv vsrgqlvavg kqnstmfslt penswtpkac vivyyieddg  541  eiisdvlkip vqlvfknkik lywskvkaep sekvslrisv tqpdsivgiv avdksvnlmn  601  asnditmenv vhelelyntg yylgmfmnsf avfqecglwv ltdanltkdy idgvydnaey  661  aerfmeeneg hivdihdfsl gssphvrkhf petwiwldtn mgyriyqefe vtvpdsitsw  721  vatgfvised lglgltttpv elqafqpffi flnlpysvir geefaleiti fnylkdatev  781  kviieksdkf dilmtsnein atghqqtllv psedgatvlf pirpthlgei pitvtalspt  841  asdavtqmil vkaegieksy sqsilldltd nrlqstlktl sfsfppntvt gservqitai  901  gdvlgpsing laslirmpyg cgeqnminfa pniyildylt kkkqltdnlk ekalsfmrqg  961  yqrellyqre dgsfsafgny dpsgstwlsa fvlrcflead pyididqnvl hrtytwlkgh 1021  qksngefwdp grvihselqg gnkspvtlta yivtsllgyr kyqpnidvqe sihflesefs 1081  rgisdnytla lityalssvg spkakealnm ltwraeqegg mqfwvssesk lsdswqprsl 1141  dievaayall shflqfqtse gipimrwlsr qrnslggfas tqdttvalka lsefaalmnt 1201  ertniqvtvt gpsspspvkf lidthnrlll qtaelavvqp tavnisangf gfaicqlnvv 1261  ynvkasgssr rrrsiqnqea fdldvavken kddlnhvdln vctsfsgpgr sgmalmevnl 1321  lsgfmvpsea islsetvkkv eydhgklnly ldsvnetqfc vnipavrnfk vsntqdasvs 1381  ivdyyeprrq avrsynsevk lsscdlcsdv qgcrpcedga sgshhhssvi fifcfkllyf 1441  melwl Protein sequence isoform 2 (SEQ ID NO (67)):    1  mqgpplltaa hllcvctaal avapgprflv tapgiirpgg nvtigvelle hcpsqvtvka   61  ellktasnlt vsvleaegvf ekgsfktltl pslplnsade iyelrvtgrt qdeilfsnst  121  rlsfetkris vfiqtdkaly kpkqevkfri vtlfsdfkpy ktslnilikd pksnliqqwl  181  sqqsdlgvis ktfqlsshpi lgdwsiqvqv ndqtyyqsfq vseyvlpkfe vtlqtplycs  241  mnskhlngti takytygkpv kgdvtltflp lsfwgkkkni tktfkingsa nfsfndeemk  301  nvmdssngls eyldlsspgp veilttvtes vtgisrnvst nvffkqhdyi ieffdyttvl  361  kpslnftatv kvtradgnql tleerrnnvv itvtqrnyte ywsgsnsgnq kmeavqkiny  421  tvpqsgtfki efpiledsse lqlkayflgs kssmavhslf kspsktyiql ktrdenikvg  481  spfelvvsgn krlkelsymv vsrgqlvavg kqnstmfslt penswtpkac vivyyieddg  541  eiisdvlkip vqlvfknkik lywskvkaep sekvslrisv tqpdsivgiv avdksvnlmn  601  asnditmenv vhelelyntg yylgmfmnsf avfqecglwv ltdanltkdy idgvydnaey  661  aerfmeeneg hivdihdfsl gssphvrkhf petwiwldtn mgyriyqefe vtvpdsitsw  721  vatgfvised lglgltttpv elqafqpffi flnlpysvir geefaleiti fnylkdatev  781  kviieksdkf dilmtsnein atghqqtllv psedgatvlf pirpthlgei pitvtalspt  841  asdavtqmil vkaegieksy sqsillditd nrlqstiktl sfsfppntvt gservqitai  901  gdvlgpsing laslirmpyg cgeqnminfa pniyildylt kkkqltdnlk ekalsfmrqg  961  yqrellyqre dgsfsafgny dpsgstwlsa fvlrcflead pyididqnvl hrtytwlkgh 1021  qksngefwdp grvihselqg gnkspvtlta yivtsllgyr kyqpnidvqe sihflesefs 1081  rgisdnytla lityalssvg spkakealnm ltwraeqegg mqfwvssesk lsdswqprsl 1141  dievaayall shflqfqtse gipimrwlsr qrnslggfas tqdttvalka lsefaalmnt 1201  ertniqvtvt gpsspsplav vqptavnisa ngfgfaicql nvvynvkasg ssrrrrsiqn 1261  qeafdldvav kenkddlnhv dinvctsfsg pgrsgmalme vnllsgfmvp seaislsetv 1321  kkveydhgkl nlyldsvnet qfcvnipavr nfkvsntqda svsivdyyep rrqavrsyns 1381  evklsscdlc sdvqgcrpce dgasgshhhs svififcfkl lyfmelwl Protein sequence isoform 3 (SEQ ID NO (68)):    1  mqgpplltaa hllcvctaal avapgprflv tapgiirpgg nvtigvelle hcpsqvtvka   61  ellktasnlt vsvleaegvf ekgsfktltl psdpksnliq qwlsqqsdlg visktfqlss  121  hpilgdwsiq vqvndqtyyq sfqvseyvlp kfevtlqtpl ycsmnskhin gtitakytyg  181  kpvkgdvtlt flplsfwgkk knitktfkin gsanfsfnde emknvmdssn glseyldlss  241  pgpveilttv tesvtgisrn vstnvffkqh dyiieffdyt tvlkpslnft atvkvtradg  301  nqltleerrn nvvitvtqrn yteywsgsns gnqkmeavqk inytvpqsgt fkiefpiled  361  sselqlkayf lgskssmavh slfkspskty iqlktrdeni kvgspfelwv sgnkrlkels  421  ymvvsrgqlv avgkqnstmf sltpenswtp kacvivyyie ddgeiisdvl kipvqlvfkn  481  kiklywskvk aepsekvslr isvtqpdsiv givavdksvn lmnasnditm enwvhelely  541  ntgyylgmfm nsfavfqecg lwvltdanlt kdyidgvydn aeyaerfmee neghivdihd  601  fslgssphvr khfpetwiwl dtnmgyriyq efevtvpdsi tswvatgfvi sedlglgltt  661  tpvelqafqp ffiflnlpys virgeefale itifnylkda tevkviieks dkfdilmtsn  721  einatghqqt llvpsedgat vlfpirpthl geipitvtal sptasdavtq milvkaegie  781  ksysqsilld ltdnrlqstl ktlsfsfppn tvtgservqi taigdvlgps inglaslirm  841  pygcgeqnmi nfapniyild yltkkkqltd nlkekalsfm rqgyqrelly qredgsfsaf  901  gnydpsgstw lsafvlrcfl eadpyididq nvlhrtytwl kghqksngef wdpgrvihse  961  lqggnkspvt ltayivtsll gyrkyqpnid vqesihfles efsrgisdny tlalityals 1021  svgspkakea lnmltwraeq eggmqfwvss esklsdswqp rsldievaay allshflqfq 1081  tsegipimrw lsrqrnslgg fastqdttva lkalsefaal mntertniqv tvtgpsspsp 1141  vkflidthnr lllqtaelav vqptavnisa ngfgfaicql nvvynvkasg ssrrrrsiqn 1201  qeafdldvav kenkddlnhv dinvctsfsg pgrsgmalme vnllsgfmvp seaislsetv 1261  kkveydhgkl nlyldsvnet qfcvnipavr nfkvsntqda svsivdyyep rrqavrsyns 1321  evklsscdlc sdvqgcrpce dgasgshhhs svififcfkl lyfmelwl Gene ID: Y7 Gene symbol: ITGB8 Gene description: integrin, beta 8 Unigene: Hs.592171 Genbank: M73780 Entrez Gene: 3696 Refseq: NM_002214 Protein sequence (SEQ ID NO (69)):    1  mcgsalafft aafvclqndr rgpasflwaa wvfslvlglg qgednrcass naascarcla   61  lgpecgwcvq edfisggsrs ercdivsnli skgcsvdsie ypsvhviipt eneintqvtp  121  gevsiqlrpg aeanfmlkvh plkkypvdly ylvdvsasmh nnieklnsvg ndlsrkmaff  181  srdfrlgfgs yvdktvspyi sihperihnq csdynldcmp phgyihvlsl tenitefeka  241  vhrqkisgni dtpeggfdam lqaavceshi gwrkeakril lvmtdqtshl aldsklagiv  301  vpndgnchlk nnvyvksttm ehpslgqlse klidnninvi favqgkqfhw ykdllpllpg  361  tiageieska anlnnlvvea yqklisevkv qvenqvqgiy fnitaicpdg srkpgmeger  421  nvtsndevlf nvtvtmkkcd vtggknyaii kpigfnetak ihihrncscq cednrgpkgk  481  cvdetfldsk cfqcdenkch fdedqfsses ckshkdqpvc sgrgvcvcgk cschkiklgk  541  vygkycekdd fscpyhhgnl caghgeceag rcqcfsgweg drcqcpsaaa qhcvnskgqv  601  csgrgtcvcg rcectdprsi grfcehcptc ytackenwnc mqclhphnls qaildqckts  661  calmeqqhyv dqtsecfssp sylriffiif ivtfligllk vliirqvilq wnsnkiksss  721  dyrvsaskkd klilqsvctr avtyrrekpe eikmdiskln ahetfrcnf Gene ID: Y8 Gene symbol: EMP2 Gene description: epithelial membrane protein 2 Unigene: Hs.531561 Genbank: BC009687 Entrez Gene: 2013 Refseq: NM_001424 Protein sequence (SEQ ID NO (70)):    1  mlvllafiia fhitsaallf iatvdnawwv gdeffadvwr ictnntnctv indsfqeyst   61  lqavqatmil stilcciaff ifvlqlfrlk qgerfvltsi iqlmsclcvm iaasiytdrr  121  edihdknakf ypvtregsyg ysyilawvaf actfisgmmy lilrkrk Gene ID: Y9 Gene symbol: FGFBP1 Gene description: fibroblast growth factor binding protein 1 Unigene: Hs.1690 Genbank: BC008910 Entrez Gene: 9982 Refseq: NM_005130 Protein sequence (SEQ ID NO (71)):    1  mkicsltlls flllaaqvll vegkkkvkng lhskvvseqk dtlgntqikq ksrpgnkgkf   61  vtkdqancrw aateqeegis lkvectqldh efscvfagnp tsclklkder vywkqvarnl  121  rsqkdicrys ktavktrvcr kdfpessikl vsstlfgntk prkektemsp rehikgkett  181  psslavtqtm atkapecved pdmanqrkta lefcgetwss lctfflsivq dtsc Gene ID: Y10 Gene symbol: CDH3 Gene description: cadherin 3, type 1, P-cadherin (placental) Unigene: Hs.191842 Genbank: BC041846 Entrez Gene: 1001 Refseq: NM_001793 Protein sequence (SEQ ID NO (72)):    1  mglprgplas llllqvcwlq caasepcrav freaevtlea ggaeqepgqa lgkvfmgcpg   61  qepalfstdn ddftvrnget vqerrslker nplkifpskr ilrrhkrdwv vapisvpeng  121  kgpfpqrinq lksnkdrdtk ifysitgpga dsppegvfav eketgwllln kpldreeiak  181  yelfghavse ngasvedpmn isiivtdqnd hkpkftqdtf rgsvlegvlp gtsvmqvtat  241  deddaiytyn gvvaysihsq epkdphdlmf tihrstgtis vissgldrek vpeytltiqa  301  tdmdgdgstt tavavveild andnapmfdp qkyeahvpen avghevqrlt vtdldapnsp  361  awratylimg gddgdhftit thpesnqgil ttrkgldfea knqhtlyvev tneapfvlkl  421  ptstativvh vedvneapvf vppskvvevq egiptgepvc vytaedpdke nqkisyrilr  481  dpagwlamdp dsgqvtavgt ldredeqfvr nniyevmvla mdngsppttg tgtllltlid  541  vndhgpvpep rqiticnqsp vrqvlnitdk dlsphtspfq aqltddsdiy wtaevneegd  601  tvvlslkkfl kqdtydvhls lsdhgnkeql tviratvcdc hghvetcpgp wkggfilpvl  661  gavlallfll lvllllvrkk rkikeplllp eddtrdnvfy ygeegggeed qdyditqlhr  721  glearpevvl rndvaptiip tpmyrprpan pdeignfiie nlkaantdpt appydtllvf  781  dyegsgsdaa slssltssas dqdqdydyln ewgsrfkkla dmygggedd Gene ID: Y11 Gene symbol: ITGB4 Gene description: integrin, beta 4 Unigene: Hs.632226 Genbank: X53587 Entrez Gene: 3691 Refseq: NM_000213|NM_001005619|NM_001005731 Protein sequence isoform 1 (SEQ ID NO (73)):    1  magprpspwa rlllaalisv slsgtlanrc kkapvkscte cvrvdkdcay ctdemfrdrr   61  cntqaellaa gcqresivvm essfqiteet qidttirrsq mspqglrvrl rpgeerhfel  121  evfeplespv dlyilmdfsn smsddldnik kmgqnlarvl sqltsdytig fgkfvdkvsv  181  pqtdmrpekl kepwpnsdpp fsfknvisit edvdefrnkl qgerisgnld apeggfdail  241  qtavctrdig wrpdsthllv fstesafhye adganvlagi msrnderchl dttgtytqyr  301  tqdypsvptl vrllakhnii pifavtnysy syyeklhtyf pvsslgvlqe dssnivelle  361  eafnrirsnl diraldsprg lrtevtskmf qktrtgsfhi rrgevgiyqv qlralehvdg  421  thvcqlpedq kgnihlkpsf sdglkmdagi icdvctcelq kevrsarcsf ngdfvcgqcv  481  csegwsgqtc nestgslsdi qpclregedk pcsgrgecqc ghcvcygegr yegqfceydn  541  fqcprtsgfl cndrgresmg qcvcepgwtg pscdcplsna tcidsnggic ngrghcecgr  601  chchqqslyt dticeinysa ihpglcedir scvqcqawgt gekkgrtcee cnfkvkmvde  661  lkraeevvvr csfrdedddc tysytmegdg apgpnstvlv hkkkdcppgs fwwlipllll  721  llpllallll lcwkycacck aclallpccn rghmvgfked hymlrenlma sdhldtpmlr  781  sgnlkgrdvv rwkvtnnmqr pgfathaasi nptelvpygl sirlaricte nilkpdtrec  841  aqlrqeveen lnevyrqisg vhklqqtkfr qqpnagkkqd htivdtvlma prsakpallk  901  ltekqveqra fhdlkvapgy ytltadqdar gmvefqegve lvdvrvplfi rpedddekql  961  lveaidvpag tatlgrrlvn itiikeqard vvsfeqpefs vsrgdqvari pvirrvldgg 1021  ksqvsyrtqd gtaqgnrdyi pvegellfqp geawkelqvk llelqevdsl lrgrqvrrfh 1081  vqlsnpkfga hlgqphstti iirdpdeldr sftsqmlssq ppphgdlgap qnpnakaags 1141  rkihfnwlpp sgkpmgyrvk ywiqgdsese ahlldskvps veltnlypyc dyemkvcayg 1201  aqgegpyssl vscrthqevp sepgrlafnv vsstvtqlsw aepaetngei tayevcyglv 1261  nddnrpigpm kkvlvdnpkn rmllienlre sqpyrytvka rngagwgper eaiinlatqp 1321  krpmsipiip dipivdaqsg edydsflmys ddvirspsgs qrpsvsddtg cgwkfepllg 1381  eeldlrrvtw rlppeliprr sassgrssda eaphgppddg gaggkggslp rsatpgppge 1441  hlvngrmdfa fpgstnslhr mtttsaaayg thisphvphr vlstsstltr dynsltrseh 1501  shsttlprdy stltsvsshd srltagvpdt ptrivfsalg ptslrvswqe prcerplqgy 1561  sveyqllngg elhrlnipnp aqtsvvvedl lpnhsyvfrv raqsqegwgr eregvities 1621  qvhpqsplcp lpgsaftist psapgplvft alspdslqls werprrpngd ivgylvtcem 1681  aqgggpataf rvdgdspesr ltvpglsenv pykfkvqart tegfgpereg iitiesqdgg 1741  pfpqlgsrag lfqhplqsey ssittthtsa tepflvdglt lgaqhleagg sltrhvtqef 1801  vsrtlttsgt lsthmdqqff qt Protein sequence isoform 2 (SEQ ID NO (74)):    1  magprpspwa rlllaalisv slsgtlanrc kkapvkscte cvrvdkdcay ctdemfrdrr   61  cntqaellaa gcqresivvm essfqiteet qidttlrrsq mspqglrvrl rpgeerhfel  121  evfeplespv dlyilmdfsn smsddldnik kmgqnlarvl sqltsdytig fgkfvdkvsv  181  pqtdmrpekl kepwpnsdpp fsfknvislt edvdefrnkl qgerisgnld apeggfdail  241  qtavctrdig wrpdsthllv fstesafhye adganvlagi msrnderchl dttgtytqyr  301  tqdypsvptl vrllakhnii pifavtnysy syyeklhtyf pvsslgvlqe dssnivelle  361  eafnrirsnl diraldsprg lrtevtskmf qktrtgsfhi rrgevgiyqv qlralehvdg  421  thvcqlpedq kgnihlkpsf sdglkmdagi icdvctcelq kevrsarcsf ngdfvcgqcv  481  csegwsgqtc ncstgslsdi qpclregedk pcsgrgecqc ghcvcygegr yegqfceydn  541  fqcprtsgfl cndrgresmg qcvcepgwtg pscdcplsna tcidsnggic ngrghcecgr  601  chchqqslyt dticeinysa ihpglcedlr scvqcqawgt gekkgrtcee cnfkvkmvde  661  lkraeevvvr csfrdedddc tysytmegdg apgpnstvlv hkkkdcppgs fwwlipllll  721  llpllallll lcwkycacck aclallpccn rghmvgfked hymlrenlma sdhldtpmlr  781  sgnlkgrdvv rwkvtnnmqr pgfathaasi nptelvpygl slrlarlcte nllkpdtrec  841  aqlrqeveen lnevyrqisg vhklqqtkfr qqpnagkkqd htivdtvlma prsakpallk  901  ltekqveqra fhdlkvapgy ytltadqdar gmvefqegve lvdvrvplfi rpedddekql  961  lveaidvpag tatlgrrlvn itiikeqard vvsfeqpefs vsrgdqvari pvirrvldgg 1021  ksqvsyrtqd gtaqgnrdyi pvegellfqp geawkelqvk llelqevdsl lrgrqvrrfh 1081  vqlsnpkfga hlgqphstti iirdpdeldr sftsqmlssq ppphgdlgap qnpnakaags 1141  rkihfnwlpp sgkpmgyrvk ywiqgdsese ahlldskvps veltnlypyc dyemkvcayg 1201  aqgegpyssl vscrthqevp sepgrlafnv vsstvtqlsw aepaetngei tayevcyglv 1261  nddnrpigpm kkvlvdnpkn rmllienlre sqpyrytvka rngagwgper eaiinlatqp 1321  krpmsipiip dipivdaqsg edydsflmys ddvlrspsgs qrpsvsddte hlvngrmdfa 1381  fpgstnslhr mtttsaaayg thisphvphr vlstsstltr dynsltrseh shsttlprdy 1441  stltsvsshg lppiwehgrs rlplswalgs rsraqmkgfp psrgprdsii lagrpaapsw 1501  gpdsrltagv pdtptrlvfs algptsirvs wqeprcerpl qgysveyqll nggelhrlni 1561  pnpaqtsvvv edllpnhsyv frvraqsqeg wgreregvit iesqvhpqsp lcplpgsaft 1621  lstpsapgpl vftalspdsl qlswerprrp ngdivgylvt cemaqgggpa tafrvdgdsp 1681  esrltvpgls envpykfkvq arttegfgpe regiitiesq dggpfpqlgs raglfqhplq 1741  seyssittth tsatepflvd gltlgaqhle aggsltrhvt qefvsrtltt sgtlsthmdq 1801  qffqt Protein sequence isoform 3 (SEQ ID NO (75)):    1  magprpspwa rlllaalisv slsgtlanrc kkapvkscte cvrvdkdcay ctdemfrdrr   61  cntqaellaa gcqresivvm essfqiteet qidttlrrsq mspqglrvrl rpgeerhfel  121  evfeplespv dlyilmdfsn smsddldnik kmgqnlarvl sqltsdytig fgkfvdkvsv  181  pqtdmrpekl kepwpnsdpp fsfknvisit edvdefrnkl qgerisgnld apeggfdail  241  qtavctrdig wrpdsthllv fstesafhye adganvlagi msrnderchl dttgtytqyr  301  tqdypsvptl vrllakhnii pifavtnysy syyeklhtyf pvsslgvlqe dssnivelle  361  eafnrirsnl diraldsprg lrtevtskmf qktrtgsfhi rrgevgiyqv qlralehvdg  421  thvcqlpedq kgnihlkpsf sdglkmdagi icdvctcelq kevrsarcsf ngdfvcgqcv  481  csegwsgqtc ncstgslsdi qpclregedk pcsgrgecqc ghcvcygegr yegqfceydn  541  fqcprtsgfl cndrgrcsmg qcvcepgwtg pscdcplsna tcidsnggic ngrghcecgr  601  chchqqslyt dticeinysa ihpglcedlr scvqcqawgt gekkgrtcee cnfkvkmvde  661  lkraeevvvr csfrdedddc tysytmegdg apgpnstvlv hkkkdcppgs fwwlipllll  721  llpllallll lcwkycacck aclallpccn rghmvgfked hymlrenlma sdhldtpmlr  781  sgnlkgrdvv rwkvtnnmqr pgfathaasi nptelvpygl sirlaricte nllkpdtrec  841  aqlrqeveen lnevyrqisg vhklqqtkfr qqpnagkkqd htivdtvlma prsakpallk  901  ltekqveqra fhdlkvapgy ytltadqdar gmvefqegve lvdvrvplfi rpedddekql  961  lveaidvpag tatlgrrlvn itiikeqard wvsfeqpefs vsrgdqvari pvirrvldgg 1021  ksqvsyrtqd gtaqgnrdyi pvegellfqp geawkelqvk llelqevdsl lrgrqvrrfh 1081  vqlsnpkfga hlgqphstti iirdpdeldr sftsqmissq ppphgdlgap qnpnakaags 1141  rkihfnwlpp sgkpmgyrvk ywiqgdsese ahlldskvps veltnlypyc dyemkvcayg 1201  aqgegpyssl vscrthqevp sepgrlafnv vsstvtqlsw aepaetngei tayevcyglv 1261  nddnrpigpm kkvlvdnpkn rmllienlre sqpyrytvka rngagwgper eaiinlatqp 1321  krpmsipiip dipivdaqsg edydsflmys ddvlrspsgs qrpsvsddte hlvngrmdfa 1381  fpgstnslhr mtttsaaayg thisphvphr vlstsstltr dynsltrseh shsttlprdy 1441  stltsvsshd srltagvpdt ptrivfsalg ptslrvswqe prcerplqgy sveyqllngg 1501  elhrlnipnp aqtsvwvedl lpnhsyvfrv raqsqegwgr eregvities qvhpqsplcp 1561  lpgsaftlst psapgplvft alspdslqls werprrpngd ivgylvtcem aqgggpataf 1621  rvdgdspesr ltvpglsenv pykfkvqart tegfgpereg iitiesqdgg pfpqlgsrag 1681  lfqhplqsey ssittthtsa tepflvdglt lgaqhleagg sltrhvtqef vsrtlttsgt 1741  lsthmdqqff qt Gene ID: Y12 Gene symbol: LAMB3 Gene description: laminin, beta 3 Unigene: Hs.497636 Genbank: BC075838 Entrez Gene: 3914 Refseq: NM_000228 Protein sequence (SEQ ID NO (76)):    1  mrpffllcfa lpgllhaqqa csrgacyppv gdllvgrtrf lrasstcglt kpetyctqyg   61  ewqmkcckcd srqphnyysh rvenvasssg pmrwwqsqnd vnpvslqldl drrfqlqevm  121  mefqgpmpag mlierssdfg ktwrvyqyla adctstfprv rqgrpqswqd vrcqslpqrp  181  narlnggkvq lnlmdlvsgi patqsqkiqe vgeitnlrvn ftrlapvpqr gyhppsayya  241  vsqlrlqgsc fchghadrca pkpgasagps tavqvhdvcv cqhntagpnc ercapfynnr  301  pwrpaegqda hecqrcdcng hsetchfdpa vfaasqgayg gvcdncrdht egkncercql  361  hyfrnrrpga siqetcisce cdpdgavpga pcdpvtgqcv ckehvqgerc dickpgftgl  421  tyanpqgchr cdcnilgsrr dmpcdeesgr clclpnvvgp kcdqcapyhw klasgqgcep  481  cacdphnsls pqcnqftgqc pcregfggim csaaairqcp drtygdvatg cracdcdfrg  541  tegpgcdkas grclcrpglt gpredqcqrg ycnrypvcva chpcfqtyda dlreqalrfg  601  rlrnataslw sgpgledrgl asrildaksk ieqiravlss pavteqevaq vasailslrr  661  tlqglqldip leeetlsipr dlesidrsfn glltmyqrkr eqfekissad psgafrmlst  721  ayeqsaqaaq qvsdssrlld qlrdsrreae rlvrqagggg gtgspklval rlemsslpdl  781  tptfnklcgn srqmactpis cpgelcpqdn gtacgsrcrg vlpraggafl magqvaeqlr  841  gfnaqlqrtr qmiraaeesa sqiqssaqrl etqvsasrsq meedvrrtrl liqqvrdflt  901  dpdtdaatiq evseavlalw lptdsatvlq kmneiqaiaa rlpnvdlvls qtkqdiarar  961  rlqaeaeear srahavegqv edvvgnlrqg tvalqeaqdt mqgtsrslrl iqdrvaevqq 1021  vlrpaeklvt smtkqlgdfw trmeelrhqa rqqgaeavqa qqlaegaseq alsaqegfer 1081  ikqkyaelkd rlgqssmlge qgariqsvkt eaeelfgetm emmdrmkdme lellrgsqai 1141  mlrsadltgl ekrveqirdh ingrvlyyat ck Gene ID: Y13 Gene symbol: CD55 Gene description: CD55 antigen Unigene: Hs.126517 Genbank: M31516 Entrez Gene: 1604 Refseq: NM_000574 Protein sequence (SEQ ID NO (77)):    1  mtvarpsvpa alpllgelpr llllvllclp avwgdcglpp dvpnaqpale grtsfpedtv   61  itykceesfv kipgekdsvi clkgsqwsdi eefcnrscev ptrinsaslk qpyitqnyfp  121  vgtvveyecr pgyrrepsls pkltclqnlk wstavefckk kscpnpgeir ngqidvpggi  181  lfgatisfsc ntgyklfgst ssfclisgss vqwsdplpec reiycpappq idngiiqger  241  dhygyrqsvt yacnkgftmi gehsiyctvn ndegewsgpp pecrgkslts kvpptvqkpt  301  tvnvpttevs ptsqktttkt ttpnaqatrs tpvsrttkhf hettpnkgsg ttsgttrlls  361  ghtcftltgl lgtlvtmgll t Gene ID: Y14 Gene symbol: CLDN16 Gene description: claudin 16 Unigene: Hs.251391 Genbank: BC069682 Entrez Gene: 10686 Refseq: NM_006580 Protein sequence (SEQ ID NO (78)):    1  mtsrtpllvt aclyysycns rhlqqgvrks krpvfshcqv petqktdtrh lsgaragvcp   61  cchpdgllat mrdllqyiac ffaffsagfl ivatwtdcwm vnaddslevs tkcrglwwec  121  vtnafdgirt cdeydsilae hplklvvtra lmitadilag fgfltlllgl dcvkflpdep  181  yikvricfva gatlliagtp giigsvwyav dvyverstlv lhniflgiqy kfgwscwlgm  241  agslgcflag avltcclylf kdvgpernyp yslrkaysaa gvsmaksysa prtetakmya  301  vdtrv Gene ID: Y15 Gene symbol: LAMA3 Gene description: laminin, alpha 3 Unigene: Hs.436367 Genbank: AY327115 Entrez Gene: 3909 Refseq: NM_198129|NM_001127717|NM_000227|NM_001127718 Protein sequence isoform 1 (SEQ ID NO (79)):    1  maaaarprgr algpvlpptp llllvlrvlp acgatardpg aaaglslhpt yfnlaeaari   61  watatcgerg pgegrpqpel ycklvggpta pgsghtiqgq fcdycnsedp rkahpvtnai  121  dgserwwqsp plssgtqynr vnltldlgql fhvayilikf ansprpdlwv lersvdfgst  181  yspwqyfahs kvdclkefgr eanmavtrdd dvlcvteysr ivplengevv vslingrpga  241  knftfshtlr eftkatnirl rflrtntllg hliskaqrdp tvtrryyysi kdisiggqcv  301  cnghaevcni nnpeklfrce cqhhtcgetc drcctgynqr rwrpaaweqs heceacnchg  361  hasncyydpd verqqasint qgiyagggvc incqhntagv nceqcakgyy rpygvpvdap  421  dgcipcscdp ehadgceqgs grchckpnfh gdncekcaig yynfpfclri pifpvstpss  481  edpvagdikg cdcnlegvlp eicdahgrcl crpgvegprc dtcrsgfysf picqacwcsa  541  lgsyqmpcss vtgqcecrpg vtgqrcdrel sgaydfphcq gsssacdpag tinsnlgycq  601  cklhvegptc srckllywnl dkenpsgcse ckchkagtvs gtgecrqgdg dchckshvgg  661  dscdtcedgy faleksnyfg cqgcqcdigg alssmcsgps gvcqcrehww gkvcqrpenn  721  yyfpdlhhmk yeiedgstpn grdlrfgfdp lafpefswrg yaqmtsvqnd vritlnvgks  781  sgslfrvilr yvnpgteavs ghitiypswg aaqskeiifl pskepafvtv pgngfadpfs  841  itpgiwvaci kaegvlldyl vilprdyyea sviqlpvtep cayagppqen cllyqhlpvt  901  rfpctlacea rhflldgepr pvavrqptpa hpvmvdlsgr evelhirlri pqvghyvvvv  961  eysteaaqlf vvdvnvkssg svlagqvniy scnysvlcrs avidhmsria myelladadi 1021  qlkghmarfl lhqvciipie efsaeyvrpq vhciasygrf vnqsatcvsl ahetpptali 1081  ldvlsgrpfp hlpqqsspsv dvlpgvtlka pqnqvtlrgr vphlgryvfv ihfyqaahpt 1141  fpaqvsvdgg wpragsfhas fcphvlgcrd qviaegqief disepevaat vkvpegkslv 1201  lvrvlvvpae nydyqilhkk smdkslefit ncgknsfyld pqtasrfckn sarslvafyh 1261  kgalpcechp tgatgphcsp eggqcpcqpn vigrqctrca tghygfprck pcscgrrice 1321  emtgqcrepp rtvrpqcevc ethsfsfhpm agcegencsr rgtieaampe cdrdsgqcrc 1381  kpritgrqcd rcasgfyrfp ecvpcncnrd gtepgvcdpg tgaclckenv egtecnvcre 1441  gsfhldpanl kgctscfcfg vnnqchsshk rrtkfvdmlg whletadrvd ipvsfnpgsn 1501  smvadlqelp atihsaswva ptsylgdkvs syggyltyqa ksfglpgdmv llekkpdvql 1561  tgqhmsiiye etntprpdrl hhgrvhvveg nfrhassrap vsreelmtvl srladvriqg 1621  lyftetqrit lsevgleeas dtgsgriala veicacppay agdscqgcsp gyyrdhkgly 1681  tgrcvpcncn ghsnqcqdgs gicvncqhnt agehcercqe gyygnavhgs cracpcphtn 1741  sfatgcvvng gdvrcsckag ytgtqcerca pgyfgnpqkf ggscqpcscn sngqlgschp 1801  ltgdcinqep kdsspaeecd dcdscvmtll ndlatmgeql rlvksqlqgl sasaglleqm 1861  rhmetqakdl rnqllnyrsa isnhgskieg lereltdlnq efetlqekaq vnsrkaqtln 1921  nnvnratqsa keldvkiknv irnvhillkq isgtdgegnn vpsgdfsrew aeaqrmmrel 1981  rnrnfgkhlr eaeadkresq lllnrirtwq kthqgenngl ansirdslne yeaklsdlra 2041  rlqeaaaqak qanglngene ralgaiqrqv keinslqsdf tkylttadss llqtnialql 2101  meksqkeyek laaslnearq elsdkvrels rsagktslve eaekharslq elakqleeik 2161  rnasgdelvr cavdaataye nilnaikaae daanraasas esalqtvike dlprkaktls 2221  snsdklinea kmtqkklkqe vspalnnlqq tlnivtvqke vidtnittlr dglhgiqrgd 2281  idamissaks mvrkanditd evldglnpiq tdverikdty grtqnedfkk altdadnsvn 2341  kltnklpdlw rkiesinqql lplgnisdnm drireliqqa rdaaskvavp mrfngksgve 2401  vrlpndledl kgytslslfl qrpnsrengg tenmfvmylg nkdasrdyig mavvdgqltc 2461  vynlgdreae lqvdqiltks etkeavmdrv kfqriyqfar lnytkgatss kpetpgvydm 2521  dgrnsntlln ldpenvvfyv ggyppdfklp srlsfppykg cielddlnen vislynfkkt 2581  fnlnttevep crrrkeesdk nyfegtgyar vptqphapip tfgqtiqttv drgllffaen 2641  gdrfislnie dgklmvrykl nselpkergv gdainngrdh siqikigklq krmwinvdvq 2701  ntiidgevfd fstyylggip iairerfnis tpafrgcmkn lkktsgwvrl ndtvgvtkkc 2761  sedwklvrsa sfsrggqlsf tdlglpptdh lqasfgfqtf qpsgilldhq twtrnlqvtl 2821  edgyielsts dsgspifksp qtymdgllhy vsvisdnsgl rlliddqllr nskrlkhiss 2881  srqslriggs nfegcisnvf vqrlslspev ldltsnsikr dvslggcsin kppflmllkg 2941  strfnktktf rinqllqdtp vasprsvkvw qdacsplpkt qanhgalqfg diptshllfk 3001  lpqellkprs qfavdmqtts srglvfhtgt knsfmalyls kgrlvfalgt dgkklriksk 3061  ekcndgkwht vvfghdgekg rlvvdglrar egslpgnsti sirapvylgs ppsgkpkslp 3121  tnsfvgclkn fqldskplyt psssfgvssc lggplekgiy fseegghvvl ahsvllgpef 3181  klvfsirprs ltgilihigs qpgkhlcvyl eagkvtasmd sgaggtstsv tpkqslcdgq 3241  whsvavtikq hilhleldtd ssytagqipf ppastqeplh lggapanltt lripvwksff 3301  gclrnihvnh ipvpvteale vqgpvslngc pdq Protein sequence isoform 3 (SEQ ID NO (80)):    1  maaaarprgr algpvlpptp llllvlrvlp acgatardpg aaaglslhpt yfnlaeaari   61  watatcgerg pgegrpqpel ycklvggpta pgsghtiqgq fcdycnsedp rkahpvtnai  121  dgserwwqsp plssgtqynr vnitldlgql fhvayilikf ansprpdlwv lersvdfgst  181  yspwqyfahs kvdclkefgr eanmavtrdd dvlcvteysr ivplengevv vslingrpga  241  knftfshtlr eftkatnirl rflrtntllg hliskaqrdp tvtrryyysi kdisiggqcv  301  cnghaevcni nnpeklfrce cqhhtcgetc drectgynqr rwrpaaweqs heceacnchg  361  hasncyydpd verqqasint qgiyagggvc incqhntagv nceqcakgyy rpygvpvdap  421  dgcipcscdp ehadgceqgs grchckpnfh gdncekcaig yynfpfclri pifpvstpss  481  edpvagdikg cdcnlegvlp eicdahgrel crpgvegprc dtcrsgfysf picqacwcsa  541  lgsyqmpcss vtgqcecrpg vtgqrcdrel sgaydfphcq gsssacdpag tinsnlgycq  601  cklhvegptc srckllywnl dkenpsgcse ckchkagtvs gtgecrqgdg dchckshvgg  661  dscdtcedgy faleksnyfg cqgcqcdigg alssmcsgps gvcqcrehww gkvcqrpenn  721  yyfpdlhhmk yeiedgstpn grdlrfgfdp lafpefswrg yaqmtsvqnd vritinvgks  781  sgslfrvilr yvnpgteavs ghitiypswg aaqskeiifl pskepafvtv pgngfadpfs  841  itpgiwvaci kaegvlldyl vilprdyyea sviqlpvtep cayagppqen cllyqhlpvt  901  rfpctlacea rhflldgepr pvavrqptpa hpvmvdlsgr evelhirlri pqvghyvvvv  961  eysteaaqlf vvdvnvkssg svlagqvniy scnysvlcrs avidhmsria myelladadi 1021  qlkghmarfl lhqvciipie efsaeyvrpq vhciasygrf vnqsatcvsl ahetpptali 1081  ldvlsgrpfp hlpqqsspsv dvlpgvtlka pqnqvtlrgr vphlgryvfv ihfyqaahpt 1141  fpaqvsvdgg wpragsfhas fcphvlgerd qviaegqief disepevaat vkvpegkslv 1201  lvrvlvvpae nydyqilhkk smdkslefit ncgknsfyld pqtasrfckn sarslvafyh 1261  kgalpcechp tgatgphcsp eggqcpcqpn vigrqctrca tghygfprck pcscgrrice 1321  emtgqcrepp rtvrpqcevc ethsfsfhpm agcegencsr rgtieaampe cdrdsgqcrc 1381  kpritgrqcd rcasgfyrfp ecvpcncnrd gtepgvcdpg tgaclckenv egtecnvcre 1441  gsfhldpanl kgctscfcfg vnnqchsshk rrtkfvdmlg whletadrvd ipvsfnpgsn 1501  smvadlqelp atihsaswva ptsylgdkvs syggyltyqa ksfglpgdmv llekkpdvql 1561  tgqhmsiiye etntprpdrl hhgrvhwveg nfrhassrap vsreelmtvl srladvriqg 1621  lyftetqrlt lsevgleeas dtgsgriala veicacppay agdscqgcsp gyyrdhkgly 1681  tgrcvpcncn ghsnqcqdgs gicvncqhnt agehcercqe gyygnavhgs cracpcphtn 1741  sfatgcvvng gdvresckag ytgtqcerca pgyfgnpqkf ggscqpcsen sngqlgschp 1801  ltgdcinqep kdsspaeecd dcdscvmtll ndlatmgeql rlvksqlqgl sasaglleqm 1861  rhmetqakdl rnqllnyrsa isnhgskieg lereltdlnq efetlqekaq vnsrkaqtln 1921  nnvnratqsa keldvkiknv irnvhmlnri rtwqkthqge nnglansird slneyeakls 1981  dlrarlqeaa aqakqangln generalgai qrqvkeinsl qsdftkyltt adssllqtni 2041  alqlmeksqk eyeklaasln earqelsdkv relsrsagkt slveeaekha rslqelakql 2101  eeikrnasgd elvrcavdaa tayenilnai kaaedaanra asasesalqt vikedlprka 2161  ktlssnsdkl lneakmtqkk lkqevspaln nlqqtinivt vqkevidtnl ttlrdglhgi 2221  qrgdidamis saksmvrkan ditdevldgl npiqtdveri kdtygrtqne dfkkaltdad 2281  nsvnkltnkl pdlwrkiesi nqqllplgni sdnmdrirel iqqardaask vavpmrfngk 2341  sgvevrlpnd ledlkgytsl slflqrpnsr enggtenmfv mylgnkdasr dyigmavvdg 2401  qltcvynlgd reaelqvdqi ltksetkeav mdrvkfqriy qfarlnytkg atsskpetpg 2461  vydmdgrnsn tllnldpenv vfyvggyppd fklpsrlsfp pykgcieldd lnenvlslyn 2521  fkktfnintt evepcrrrke esdknyfegt gyarvptqph apiptfgqti qttvdrgllf 2581  faengdrfis lniedgklmv ryklnselpk ergvgdainn grdhsiqiki gklqkrmwin 2641  vdvqntiidg evfdfstyyl ggipiairer fnistpafrg cmknlkktsg wvrindtvgv 2701  tkkcsedwkl vrsasfsrgg qlsftdlglp ptdhlqasfg fqtfqpsgil ldhqtwtrnl 2761  qvtledgyie lstsdsgspi fkspqtymdg llhyvsvisd nsglrllidd qllrnskrik 2821  hisssrqslr lggsnfegci snvfvqrlsl spevldltsn slkrdvslgg cslnkppflm 2881  llkgstrfnk tktfrinqll qdtpvasprs vkvwqdacsp lpktqanhga lqfgdiptsh 2941  llfklpqell kprsqfavdm qttssrglvf htgtknsfma lylskgrlvf algtdgkklr 3001  ikskekcndg kwhtvvfghd gekgrlvvdg lraregslpg nstisirapv ylgsppsgkp 3061  kslptnsfvg clknfqldsk plytpsssfg vssclggple kgiyfseegg hvvlahsvll 3121  gpefklvfsi rprsltgili higsqpgkhl cvyleagkvt asmdsgaggt stsvtpkqsl 3181  cdgqwhsvav tikqhilhle ldtdssytag qipfppastq eplhlggapa nlttlripvw 3241  ksffgclrni hvnhipvpvt ealevqgpvs lngcpdq Protein sequence isoform 2 (SEQ ID NO (81)):    1  mppavrrsac smgwlwifga algqclgyss qqqrvpflqp pgqsqlqasy vefrpsqgcs   61  pgyyrdhkgl ytgrcvpcnc nghsnqcqdg sgicvncqhn tagehcercq egyygnavhg  121  scracpcpht nsfatgcvvn ggdvrcscka gytgtqcerc apgyfgnpqk fggscqpcsc  181  nsngqlgsch pltgdcinqe pkdsspaeec ddcdscvmtl lndlatmgeq lrlvksqlqg  241  lsasaglleq mrhmetqakd lrnqllnyrs aisnhgskie glereltdln qefetlqeka  301  qvnsrkaqtl nnnvnratqs akeldvkikn virnvhillk qisgtdgegn nvpsgdfsre  361  waeaqrmmre lrnrnfgkhl reaeadkres qlllnrirtw qkthqgenng lansirdsln  421  eyeaklsdlr arlqeaaaqa kqanglngen eralgaiqrq vkeinslqsd ftkylttads  481  sllqtnialq lmeksqkeye klaaslnear qelsdkvrel srsagktslv eeaekharsl  541  qelakqleei krnasgdelv rcavdaatay enilnaikaa edaanraasa sesalqtvik  601  edlprkaktl ssnsdkllne akmtqkklkq evspalnnlq qtlnivtvqk evidtnlttl  661  rdglhgiqrg didamissak smvrkandit devldglnpi qtdverikdt ygrtqnedfk  721  kaltdadnsv nkltnklpdl wrkiesinqq llplgnisdn mdrireliqq ardaaskvav  781  pmrfngksgv evripndled lkgytslslf lqrpnsreng gtenmfvmyl gnkdasrdyi  841  gmavvdgqlt cvynlgdrea elqvdqiltk setkeavmdr vkfqriyqfa rlnytkgats  901  skpetpgvyd mdgrnsntll nldpenvvfy vggyppdfkl psrlsfppyk gcielddlne  961  nvlslynfkk tfnlntteve pcrrrkeesd knyfegtgya rvptqphapi ptfgqtiqtt 1021  vdrgliffae ngdrfislni edgklmvryk lnselpkerg vgdainngrd hsiqikigkl 1081  qkrmwinvdv qntiidgevf dfstyylggi piairerfni stpafrgcmk nlkktsgvvr 1141  lndtvgvtkk csedwklvrs asfsrggqls ftdlglpptd hlqasfgfqt fqpsgilldh 1201  qtwtrnlqvt ledgyielst sdsgspifks pqtymdgllh yvsvisdnsg lrlliddqll 1261  rnskrikhis ssrqslrlgg snfegcisnv fvqrlslspe vldltsnslk rdvslggcsl 1321  nkppflmilk gstrfnktkt frinqllqdt pvasprsvkv wqdacsplpk tqanhgalqf 1381  gdiptshllf klpqellkpr sqfavdmqtt ssrglvfhtg tknsfmalyl skgrlvfalg 1441  tdgkklriks kekcndgkwh tvvfghdgek grlvvdglra regslpgnst isirapvylg 1501  sppsgkpksl ptnsfvgclk nfqldskply tpsssfgvss clggplekgi yfseegghvv 1561  lahsvllgpe fklvfsirpr sltgilihig sqpgkhlcvy leagkvtasm dsgaggtsts 1621  vtpkqslcdg qwhsvavtik qhilhleldt dssytagqip fppastqepl hlggapanlt 1681  tlripvwksf fgclrnihvn hipvpvteal evqgpvslng cpdq Protein sequence isoform 4 (SEQ ID NO (82)):    1  mppavrrsac smgwlwifga algqclgyss qqqrvpflqp pgqsqlqasy vefrpsqgcs   61  pgyyrdhkgl ytgrcvpcnc nghsnqcqdg sgicvncqhn tagehcercq egyygnavhg  121  scracpcpht nsfatgcvvn ggdvrescka gytgtqcerc apgyfgnpqk fggscqpcsc  181  nsngqlgsch pltgdcinqe pkdsspaeec ddcdscvmtl lndlatmgeq lrlvksqlqg  241  lsasaglleq mrhmetqakd lrnqllnyrs aisnhgskie glereltdln qefetlqeka  301  qvnsrkaqtl nnnvnratqs akeldvkikn virnvhmlnr irtwqkthqg ennglansir  361  dslneyeakl sdlrarlqea aaqakqangl ngeneralga iqrqvkeins lqsdftkylt  421  tadssllqtn ialqlmeksq keyeklaasl nearqelsdk vrelsrsagk tslveeaekh  481  arslqelakq leeikrnasg delvrcavda atayenilna ikaaedaanr aasasesalq  541  tvikediprk aktlssnsdk llneakmtqk klkqevspal nnlqqtlniv tvqkevidtn  601  lttlrdglhg iqrgdidami ssaksmvrka nditdevldg lnpiqtdver ikdtygrtqn  661  edfkkaltda dnsvnkltnk lpdlwrkies inqqllplgn isdnmdrire liqqardaas  721  kvavpmrfng ksgvevripn dledlkgyts lslflqrpns renggtenmf vmylgnkdas  781  rdyigmavvd gqltcvynlg dreaelqvdq iltksetkea vmdrvkfqri yqfarlnytk  841  gatsskpetp gvydmdgrns ntlinldpen vvfyvggypp dfklpsrlsf ppykgcield  901  dlnenvisly nfkktfnint tevepcrrrk eesdknyfeg tgyarvptqp hapiptfgqt  961  iqttvdrgll ffaengdrfi slniedgklm vryklnselp kergvgdain ngrdhsiqik 1021  igklqkrmwi nvdvqntiid gevfdfstyy lggipiaire rfnistpafr gcmknlkkts 1081  gvvrindtvg vtkkcsedwk lvrsasfsrg gqlsftdlgl pptdhlqasf gfqtfqpsgi 1141  lldhqtwtrn lqvtledgyi elstsdsgsp ifkspqtymd gllhyvsvis dnsgirllid 1201  dqllrnskrl khisssrqsl riggsnfegc isnvfvqrls lspevidits nslkrdvslg 1261  gcslnkppfl mllkgstrfn ktktfrinql lqdtpvaspr svkvwqdacs plpktqanhg 1321  alqfgdipts hilfklpqel lkprsqfavd mqttssrglv fhtgtknsfm alylskgrlv 1381  falgtdgkkl rikskekcnd gkwhtvvfgh dgekgrlvvd glraregslp gnstisirap 1441  vylgsppsgk pkslptnsfv gclknfqlds kplytpsssf gvssclggpl ekgiyfseeg 1501  ghvvlahsvl lgpefklvfs irprsltgil ihigsqpgkh lcvyleagkv tasmdsgagg 1561  tstsvtpkqs lcdgqwhsva vtikqhilhl eldtdssyta gqipfppast qeplhlggap 1621  anlttlripv wksffgclrn ihvnhipvpv tealevqgpv slngcpdq Gene ID: Y16 Gene symbol: CD40 Gene description: CD40 molecule Unigene: Hs.472860 Genbank: AB209660 Entrez Gene: 958 Refseq: NM_001250|NM_152854 Protein sequence isoform 1 (SEQ ID NO (83)):    1  mvrlplqcvl wgclltavhp epptacrekq ylinsqccsl cqpgqklvsd cteftetecl   61  pcgesefldt wnrethchqh kycdpnlglr vqqkgtsetd tictceegwh ctseacescv  121  lhrscspgfg vkqiatgvsd ticepcpvgf fsnvssafek chpwtscetk dlvvqqagtn  181  ktdvvcgpqd rlralvvipi ifgilfaill vlvfikkvak kptnkaphpk qepqeinfpd  241  dlpgsntaap vqetlhgcqp vtqedgkesr isvqerq Protein sequence isoform 2 (SEQ ID NO (84)):    1  mvrlplqcvl wgclltavhp epptacrekq ylinsqccsl cqpgqklvsd cteftetecl   61  pcgesefldt wnrethchqh kycdpnlglr vqqkgtsetd tictceegwh ctseacescv  121  lhrscspgfg vkqiatgvsd ticepcpvgf fsnvssafek chpwtrspgs aespggdphh  181  lrdpvchplg aglyqkggqe anq Gene ID: Y17 Gene symbol: COL17A1 Gene description: collagen, type XVII, alpha 1 Unigene: Hs.117938 Genbank: AL138761 Entrez Gene: 1308 Refseq: NM_000494 Protein sequence (SEQ ID NO (85)):    1  mdvtkknkrd gtevterivt etvttrltsl ppkggtsngy aktaslgggs rlekqslthg   61  ssgyinstgs trghastssy rrahspastl pnspgstfer kthvtrhaye gsssgnsspe  121  yprkefasss trgrsqtres eirvrlqsas pstrwteldd vkrllkgsrs asvsptrnss  181  ntlpipkkgt vetkivtass qsvsgtydat ildanlpshv wsstlpagss mgtyhnnmtt  241  qsssllntna ysagsvfgvp nnmascsptl hpglstsssv fgmqnnlaps lttlshgttt  301  tstaygvkkn mpqspaavnt gvstsaactt svqsddllhk dckflilekd ntpakkemel  361  limtkdsgkv ftaspasiaa tsfsedtikk ekqaaynads glkaeangdl ktvstkgktt  421  tadihsygss ggggsggggg vggagggpwg papawcpcgs ccswwkwllg llltwilllg  481  llfglialae evrklkarvd elerirrsil pygdsmdrie kdrlqgmapa agadldkigl  541  hsdsqeelwm fvrkkimmeq engnirgspg pkgdmgspgp kgdrgfpgtp gipgplghpg  601  pqgpkgqkgs vgdpgmegpm gqrgregpmg prgeagppgs gekgergaag epgphgppgv  661  pgsvgpkgss gspgpqgppg pvglqgirge vglpgvkgdk gpmgppgpkg dqgekgprgl  721  tgepgmrglp gavgepgakg amgpagpdgh qgprgeqglt gmpgirgppg psgdpgkpgl  781  tgpqgpqglp gtpgrpgikg epgapgkivt segssmltvp gppgppgamg ppgppgapgp  841  agpaglpghq eviniqgppg ppgprgppgp sipgppgprg ppgeglpgpp gppgsflsns  901  etflsgppgp pgppgpkgdq gppgprghqg eqglpgfsts gsssfginiq gppgppgpqg  961  pkgdkgdpgv pgalgipsgp seggssstmy vsgppgppgp pgppgsisss gqeiqqyise 1021  ymqsdsirsy lsgvqgppgp pgppgpvtti tgetfdysel ashvvsylrt sgygvslfss 1081  sissedilav lqrddvrqyl rqylmgprgp pgppgasgdg sllsldyael ssrilsymss 1141  sgisiglpgp pgppglpgts yeellsllrg sefrgivgpp gppgppgipg nvwssisved 1201  lssylhtagl sfipgppgpp gppgprgppg vsgalatyaa ensdsfrsel isyltspdvr 1261  sfivgppgpp gpqgppgdsr llstdashsr gssssshsss vrrgssysss mstggggags 1321  lgaggafgea agdrgpygtd igpgggygaa aeggmyagng gllgadfagd ldynelavrv 1381  sesmqrqgll qgmaytvqgp pgqpgpqgpp giskvfsays nvtadlmdff qtygaiqgpp 1441  gqkgemgtpg pkgdrgpagp pghpgppgpr ghkgekgdkg dqvyagrrrr rsiavkp Gene ID: Y18 Gene symbol: DSC2 Gene description: Desmocollin-2 Unigene: Hs.95612 Genbank: BC063291 Entrez Gene: 1824 Refseq: NM_024422|NM_004949 Protein sequence isoform Dsc2a (SEQ ID NO (86)):    1  meaarpsgsw ngalcrllll tlailifasd acknvtlhvp skldaeklvg rvnlkecfta   61  anlihssdpd fqiledgsvy ttntillsse krsftillsn tenqekkkif vflehqtkvl  121  kkrhtkekvl rrakrrwapi pcsmlenslg pfplflqqvq sdtaqnytiy ysirgpgvdq  181  eprnlfyver dtgnlyctrp vdreqyesfe iiafattpdg ytpelplpli ikiedendny  241  pifteetytf tifencrvgt tvgqvcatdk depdtmhtrl kysiigqvpp sptlfsmhpt  301  tgvitttssq ldrelidkyq lkikvqdmdg qyfglqttst ciiniddvnd hlptftrtsy  361  vtsveentvd veilrvtved kdlvntanwr anytilkgne ngnfkivtda ktnegvlcvv  421  kplnyeekqq milqigvvne apfsreaspr samstatvtv nvedqdegpe cnppiqtvrm  481  kenaevgtts ngykaydpet rsssgirykk ltdptgwvti dentgsikvf rsldreaeti  541  kngiynitvl asdqggrtct gtlgiilqdv ndnspfipkk tviickptms saeivavdpd  601  epihgppfdf slesstsevq rmwrlkaind taarlsyqnd ppfgsyvvpi tvrdrlgmss  661  vtsldvtlcd citendcthr vdpriggggv qlgkwailai llgiallfci lftlvcgasg  721  tskqpkvipd dlaqqnlivs nteapgddkv ysangfttqt vgasaqgvcg tvgsgikngg  781  qetiemvkgg hqtsescrga ghhhtldscr gghtevdncr ytysewhsft qprlgekvyl  841  cnqdenhkha qdyvltynye grgsvagsvg ccserqeedg lefldnlepk frtlaeacmk  901  r Protein sequence isoform Dsc2b (SEQ ID NO (87)):    1  meaarpsgsw ngalcrllll tlailifasd acknvtlhvp skldaeklvg rvnlkecfta   61  anlihssdpd fqiledgsvy ttntillsse krsftillsn tenqekkkif vflehqtkvl  121  kkrhtkekvl rrakrrwapi pcsmlenslg pfplflqqvq sdtaqnytiy ysirgpgvdq  181  eprnlfyver dtgnlyctrp vdreqyesfe iiafattpdg ytpelplpli ikiedendny  241  pifteetytf tifencrvgt tvgqvcatdk depdtmhtrl kysiigqvpp sptlfsmhpt  301  tgvitttssq ldrelidkyq lkikvqdmdg qyfglqttst ciiniddvnd hlptftrtsy  361  vtsveentvd veilrvtved kdlvntanwr anytilkgne ngnfkivtda ktnegvlcwv  421  kplnyeekqq milqigvvne apfsreaspr samstatvtv nvedqdegpe cnppiqtvrm  481  kenaevgtts ngykaydpet rsssgirykk ltdptgwvti dentgsikvf rsldreaeti  541  kngiynitvl asdqggrtct gtlgiilqdv ndnspfipkk tviickptms saeivavdpd  601  epihgppfdf slesstsevq rmwrlkaind taarlsyqnd ppfgsyvvpi tvrdilgmss  661  vtsldvtlcd citendcthr vdpriggggv qlgkwailai llgiallfci lftlvcgasg  721  tskqpkvipd dlaqqnlivs nteapgddkv ysangfttqt vgasaqgvcg tvgsgikngg  781  qetiemvkgg hqtsescrga ghhhtldscr gghtevdncr ytysewhsft qprlgeesir  841  ghtlikn Gene ID: Y19 Gene symbol: DSC1 Gene description: Desmocollin-1 Unigene: Hs.567260 Genbank: X72925 Entrez Gene: 1823 Refseq: NM_024421|NM_004948 Protein sequence isoform Dsc1a (SEQ ID NO (88)):    1  malasaapgs ifckqllfsl lvltllcdac qkvylrvpsh lqaetlvgkv nleeclksas   61  lirssdpafr iledgsiytt hdlilsserk sfsiflsdgq rreqqeikvv lsarenkspk  121  krhtkdtalk rskrrwapip aslmenslgp fpqhvqqiqs daaqnytify sisgpgvdke  181  pfnlfyiekd tgdifctrsi drekyeqfal ygyattadgy apeyplplii kieddndnap  241  yfehrvtift vpencrsgts vgkvtatdld epdtlhtrlk ykilqqipdh pkhfsihpdt  301  gvittttpfl drekcdtyql imevrdmggq pfglfntgti tisledendn ppsftetsyv  361  teveenridv eilrmkvqdq dlpntphska vykilqgnen gnfiistdpn tnegvlcvvk  421  plnyevnrqv ilqvgvinea qfskaassqt ptmctttvtv kiidsdegpe chppvkviqs  481  qdgfpagqel lgykaldpei ssgeglryqk lgdednwfei nqhtgdlrtl kvldreskfv  541  knnqynisvv avdavgrsct gtlvvhlddy ndhapqidke vticqnnedf avlkpvdpdg  601  pengppfqff ldnsasknwn ieekdgktai lrqrqnldyn yysvpiqikd rhglvathml  661  tvrvcdcstp secrmkdkst rdvrpnvilg rwailamvig svlllcilft cfcvtakrtv  721  kkcfpediaq qnlivsnteg pgeevteani rlpmqtsnic dtsmsvgtvg gqgiktqqsf  781  emvkggytld snkggghqtl esvkgvgqgd tgryaytdwq sftqprigek vylcgqdeeh  841  khcedyvcsy nyegkgslag svgccsdrqe eeglefldhl epkfrtlakt cikk Protein sequence isoform Dsc1b (SEQ ID NO (89)):    1  malasaapgs ifckqllfsl lvltllcdac qkvylrvpsh lqaetlvgkv nleeclksas   61  lirssdpafr iledgsiytt hdlilsserk sfsiflsdgq rreqqeikvv lsarenkspk  121  krhtkdtalk rskrrwapip aslmenslgp fpqhvqqiqs daaqnytify sisgpgvdke  181  pfnlfyiekd tgdifctrsi drekyeqfal ygyattadgy apeyplplii kieddndnap  241  yfehrvtift vpencrsgts vgkvtatdld epdtlhtrik ykilqqipdh pkhfsihpdt  301  gvittttpfl drekcdtyql imevrdmggq pfglfntgti tisledendn ppsftetsyv  361  teveenridv eilrmkvqdq dlpntphska vykilqgnen gnfiistdpn tnegvlcvvk  421  plnyevnrqv ilqvgvinea qfskaassqt ptmctttvtv kiidsdegpe chppvkviqs  481  qdgfpagqel lgykaldpei ssgeglryqk lgdednwfei nqhtgdirtl kvldreskfv  541  knnqynisvv avdavgrsct gtlvvhiddy ndhapqidke vticqnnedf avlkpvdpdg  601  pengppfqff ldnsasknwn ieekdgktai lrqrqnldyn yysvpiqikd rhglvathml  661  tvrvcdcstp secrmkdkst rdvrpnvilg rwailamvlg svlllcilft cfcvtakrtv  721  kkcfpediaq qnlivsnteg pgeevteani rlpmqtsnic dtsmsvgtvg gqgiktqqsf  781  emvkggytld snkggghqtl esvkgvgqgd tgryaytdwq sftqprlgee sirghtlikn Gene ID: Y20 Gene symbol: ITGA6 Gene description: Integrin alpha-6 Unigene: Hs.133397 Genbank: X59512 Entrez Gene: 3655 Refseq: NM_000210|NM_001079818 Protein sequence isoform b (SEQ ID NO (90)):    1  maaagqlcll ylsagllsrl gaafnldtre dnvirkygdp gslfgfslam hwqlqpedkr   61  lllvgaprae alplqranrt gglyscdita rgpctriefd ndadptsesk edqwmgvtvq  121  sqgpggkvvt cahryekrqh vntkqesrdi fgrcyvlsqn lrieddmdgg dwsfcdgrlr  181  ghekfgscqq gvaatftkdf hyivfgapgt ynwkgivrve qknntffdmn ifedgpyevg  241  getehdeslv pvpansylgf sldsgkgivs kdeitfvsga pranhsgavv llkrdmksah  301  llpehifdge glassfgydv avvdinkdgw qdivigapqy fdrdgevgga vyvymnqqgr  361  wnnvkpirln gtkdsmfgia vknigdinqd gypdiavgap yddlgkvfiy hgsangintk  421  ptqvlkgisp yfgysiagnm dldrnsypdv avgslsdsvt ifrsrpvini qktitvtpnr  481  idlrqktacg apsgiclqvk scfeytanpa gynpsisivg tleaekerrk sglssrvqfr  541  nqgsepkytq eltlkrqkqk vcmeetlwlq dnirdklrpi pitasveiqe pssrrrvnsl  601  pevlpilnsd epktahidvh flkegcgddn vcnsnlkley kfctregnqd kfsylpiqkg  661  vpelvikdqk dialeitvtn spsnprnptk dgddaheakl iatfpdtlty sayrelrafp  721  ekqlscvanq ngsqadcelg npfkrnsnvt fylvisttev tfdtpdldin lklettsnqd  781  nlapitakak vvielllsvs gvakpsqvyf ggtvvgeqam ksedevgsli eyefrvinlg  841  kpltnlgtat lniqwpkeis ngkwllylvk veskglekvt cepqkeinsl nlteshnsrk  901  kreitekqid dnrkfslfae rkyqtlncsv nvncvnircp lrgldskasl ilrsrlwnst  961  fleeysklny ldilmrafid vtaaaenirl pnagtqvrvt vfpsktvagy sgvpwwiilv 1021  ailagilmla llvfilwkcg ffkrnkkdhy datyhkaeih aqpsdkerlt sda Protein sequence isoform a (SEQ ID NO (91)):    1  maaagqlcll ylsagllsrl gaafnldtre dnvirkygdp gslfgfslam hwqlqpedkr   61  lllvgaprae alplqranrt gglyscdita rgpctriefd ndadptsesk edqwmgvtvq  121  sqgpggkvvt cahryekrqh vntkqesrdi fgrcyvlsqn lrieddmdgg dwsfcdgnlr  181  ghekfgscqq gvaatftkdf hyivfgapgt ynwkgivrve qknntffdmn ifedgpyevg  241  getehdeslv pvpansylgf sldsgkgivs kdeitfvsga pranhsgavv llkrdmksah  301  llpehifdge glassfgydv avvdlnkdgw qdivigapqy fdrdgevgga vyvymnqqgr  361  wnnvkpirln gtkdsmfgia vknigdinqd gypdiavgap yddlgkvfiy hgsangintk  421  ptqvlkgisp yfgysiagnm dldrnsypdv avgslsdsvt ifrsrpvini qktitvtpnr  481  idlrqktacg apsgiclqvk scfeytanpa gynpsisivg tleaekerrk sglssrvqfr  541  nqgsepkytq eltlkrqkqk vcmeetlwlq dnirdkirpi pitasveiqe pssrrrvnsl  601  pevlpilnsd epktahidvh flkegcgddn vcnsnlkley kfctregnqd kfsylpiqkg  661  vpelvikdqk dialeitvtn spsnprnptk dgddaheakl iatfpdtlty sayrelrafp  721  ekqlscvanq ngsqadcelg npfkrnsnvt fylvlsttev tfdtpdldin lklettsnqd  781  nlapitakak vvielllsvs gvakpsqvyf ggtvvgeqam ksedevgsli eyefrvinlg  841  kpltnigtat lniqwpkeis ngkwllylvk veskglekvt cepqkeinsl nlteshnsrk  901  kreitekqid dnrkfslfae rkyqtlncsv nvncvnircp lrgldskasl ilrsrlwnst  961  fleeysklny ldilmrafid vtaaaenirl pnagtqvrvt vfpsktvagy sgvpwwiilv 1021  ailagilmla llvfilwkcg ffkrsrydds vpryhavrir keereikdek yidnlekkqw 1081  itkwnenesy s Gene ID: Y21 Gene symbol: ITGB4 Gene description: Integrin beta-4 Unigene: Hs.632226 Genbank: X53587|X51841|X52186 Entrez Gene: 3691 Refseq: NM_000213|NM_001005619|NM_001005731 Protein sequence isoform 1 (SEQ ID NO (92)):    1  magprpspwa rlllaalisv slsgtlanrc kkapvkscte cvrvdkdcay ctdemfrdrr   61  cntqaellaa gcqresivvm essfqiteet qidttlrrsq mspqglrvrl rpgeerhfel  121  evfeplespv dlyilmdfsn smsddldnik kmgqnlarvl sqltsdytig fgkfvdkvsv  181  pqtdmrpekl kepwpnsdpp fsfknvisit edvdefrnkl qgerisgnld apeggfdail  241  qtavctrdig wrpdsthllv fstesafhye adganvlagi msrnderchl dttgtytqyr  301  tqdypsvptl vrllakhnii pifavtnysy syyeklhtyf pvsslgvlqe dssnivelle  361  eafnrirsnl diraldsprg lrtevtskmf qktrtgsfhi rrgevgiyqv qlralehvdg  421  thvcqlpedq kgnihlkpsf sdglkmdagi icdvctcelq kevrsarcsf ngdfvegqcv  481  csegwsgqtc ncstgslsdi qpclregedk pcsgrgecqc ghcvcygegr yegqfceydn  541  fqcprtsgfl cndrgresmg qcvcepgwtg pscdcplsna tcidsnggic ngrghcecgr  601  chchqqslyt dticeinysa ihpglcedlr scvqcqawgt gekkgrtcee cnfkvkmvde  661  lkraeevvvr csfrdedddc tysytmegdg apgpnstvlv hkkkdcppgs fwwlipllll  721  llpllallll lcwkycacck aclallpccn rghmvgfked hymlrenlma sdhldtpmlr  781  sgnlkgrdvv rwkvtnnmqr pgfathaasi nptelvpygl slrlarlcte nllkpdtrec  841  aqlrqeveen lnevyrqisg vhklqqtkfr qqpnagkkqd htivdtvlma prsakpallk  901  ltekqveqra fhdlkvapgy ytltadqdar gmvefqegve lvdvrvplfi rpedddekql  961  lveaidvpag tatlgrrlvn itiikeqard vvsfeqpefs vsrgdqvari pvirrvldgg 1021  ksqvsyrtqd gtaqgnrdyi pvegellfqp geawkelqvk llelqevdsl lrgrqvrrfh 1081  vqlsnpkfga hlgqphstti iirdpdeldr sftsqmlssq ppphgdlgap qnpnakaags 1141  rkihfnwlpp sgkpmgyrvk ywiqgdsese ahlldskvps veltnlypyc dyemkvcayg 1201  aqgegpyssl vscrthqevp sepgrlafnv vsstvtqlsw aepaetngei tayevcyglv 1261  nddnrpigpm kkvlvdnpkn rmllienlre sqpyrytvka rngagwgper eaiinlatqp 1321  krpmsipiip dipivdaqsg edydsflmys ddvirspsgs qrpsvsddtg cgwkfepllg 1381  eeldlrrvtw rlppeliprl sassgrssda eaphgppddg gaggkggslp rsatpgppge 1441  hlvngrmdfa fpgstnslhr mtttsaaayg thlsphvphr vlstsstltr dynsltrseh 1501  shsttlprdy stltsvsshd srltagvpdt ptrlvfsalg ptslrvswqe prcerplqgy 1561  sveyqllngg elhrlnipnp aqtsvvvedl lpnhsyvfrv raqsqegwgr eregvities 1621  qvhpqsplcp lpgsaftlst psapgplvft alspdslqls werprrpngd ivgylvtcem 1681  aqgggpataf rvdgdspesr ltvpglsenv pykfkvqart tegfgpereg iitiesqdgg 1741  pfpqlgsrag lfqhplqsey ssittthtsa tepflvdglt lgaqhleagg sltrhvtqef 1801  vsrtlttsgt lsthmdqqff qt Protein sequence isoform 2 (SEQ ID NO (93)):    1  magprpspwa rlllaalisv slsgtlanrc kkapvkscte cvrvdkdcay ctdemfrdrr   61  cntqaellaa gcqresivvm essfqiteet qidttlrrsq mspqglrvrl rpgeerhfel  121  evfeplespv dlyilmdfsn smsddldnik kmgqnlarvl sqltsdytig fgkfvdkvsv  181  pqtdmrpekl kepwpnsdpp fsfknvisit edvdefrnkl qgerisgnld apeggfdail  241  qtavctrdig wrpdsthllv fstesafhye adganvlagi msrnderchl dttgtytqyr  301  tqdypsvptl vrllakhnii pifavtnysy syyeklhtyf pvsslgvlqe dssnivelle  361  eafnrirsnl diraldsprg lrtevtskmf qktrtgsfhi rrgevgiyqv qlralehvdg  421  thvcqlpedq kgnihlkpsf sdglkmdagi icdvctcelq kevrsarcsf ngdfvcgqcv  481  csegwsgqtc ncstgslsdi qpclregedk pcsgrgecqc ghcvcygegr yegqfceydn  541  fqcprtsgfl cndrgresmg qcvcepgwtg pscdcplsna tcidsnggic ngrghcecgr  601  chchqqslyt dticeinysa ihpglcedir scvqcqawgt gekkgrtcee cnfkvkmvde  661  lkraeevvvr csfrdedddc tysytmegdg apgpnstvlv hkkkdcppgs fwwlipllll  721  llpllallll lcwkycacck aclallpccn rghmvgfked hymlrenlma sdhldtpmlr  781  sgnlkgrdvv rwkvtnnmqr pgfathaasi nptelvpygl sirlaricte nllkpdtrec  841  aqlrqeveen lnevyrqisg vhklqqtkfr qqpnagkkqd htivdtvlma prsakpallk  901  ltekqveqra fhdlkvapgy ytltadqdar gmvefqegve lvdvrvplfi rpedddekql  961  lveaidvpag tatlgrrlvn itiikeqard vvsfeqpefs vsrgdqvari pvirrvldgg 1021  ksqvsyrtqd gtaqgnrdyi pvegellfqp geawkelqvk llelqevdsl lrgrqvrrfh 1081  vqlsnpkfga hlgqphstti iirdpdeldr sftsqmissq ppphgdlgap qnpnakaags 1141  rkihfnwlpp sgkpmgyrvk ywiqgdsese ahlldskvps veltnlypyc dyemkvcayg 1201  aqgegpyssl vscrthqevp sepgrlafnv vsstvtqlsw aepaetngei tayevcyglv 1261  nddnrpigpm kkvlvdnpkn rmllienlre sqpyrytvka rngagwgper eaiinlatqp 1321  krpmsipiip dipivdaqsg edydsflmys ddvlrspsgs qrpsvsddte hlvngrmdfa 1381  fpgstnslhr mtttsaaayg thisphvphr vistsstltr dynsltrseh shsttlprdy 1441  stltsvsshg lppiwehgrs rlplswalgs rsraqmkgfp psrgprdsii lagrpaapsw 1501  gpdsrltagv pdtptrlvfs algptsirvs wqeprcerpl qgysveyqll nggelhrlni 1561  pnpaqtsvvv edllpnhsyv frvraqsqeg wgreregvit iesqvhpqsp lcplpgsaft 1621  lstpsapgpl vftalspdsl qlswerprrp ngdivgylvt cemaqgggpa tafrvdgdsp 1681  esrltvpgls envpykfkvq arttegfgpe regiitiesq dggpfpqlgs raglfqhplq 1741  seyssittth tsatepflvd gltlgaqhle aggsltrhvt qefvsrtltt sgtlsthmdq 1801  qffqt Protein sequence isoform 3 (SEQ ID NO (94)):    1  magprpspwa rlllaalisv slsgtlanrc kkapvkscte cvrvdkdcay ctdemfrdrr   61  cntqaellaa gcqresivvm essfqiteet qidttlrrsq mspqglrvrl rpgeerhfel  121  evfeplespv dlyilmdfsn smsddldnlk kmgqnlarvl sqltsdytig fgkfvdkvsv  181  pqtdmrpekl kepwpnsdpp fsfknvislt edvdefrnkl qgerisgnld apeggfdail  241  qtavctrdig wrpdsthllv fstesafhye adganvlagi msrnderchl dttgtytqyr  301  tqdypsvptl vrllakhnii pifavtnysy syyeklhtyf pvsslgvlqe dssnivelle  361  eafnrirsnl diraldsprg lrtevtskmf qktrtgsfhi rrgevgiyqv qlralehvdg  421  thvcqlpedq kgnihlkpsf sdglkmdagi icdvctcelq kevrsarcsf ngdfvcgqcv  481  csegwsgqtc ncstgslsdi qpclregedk pcsgrgecqc ghcvcygegr yegqfceydn  541  fqcprtsgfl cndrgresmg qcvcepgwtg pscdcplsna tcidsnggic ngrghcecgr  601  chchqqslyt dticeinysa ihpglcedir scvqcqawgt gekkgrtcee cnfkvkmvde  661  lkraeevvvr csfrdedddc tysytmegdg apgpnstvlv hkkkdcppgs fwwlipllll  721  llpllallll lcwkycacck aclallpccn rghmvgfked hymlrenlma sdhldtpmlr  781  sgnlkgrdvv rwkvtnnmqr pgfathaasi nptelvpygl slrlarlcte nllkpdtrec  841  aqlrqeveen lnevyrqisg vhklqqtkfr qqpnagkkqd htivdtvlma prsakpallk  901  ltekqveqra fhdlkvapgy ytltadqdar gmvefqegve lvdvrvplfi rpedddekql  961  lveaidvpag tatlgrrlvn itiikeqard vvsfeqpefs vsrgdqvari pvirrvldgg 1021  ksqvsyrtqd gtaqgnrdyi pvegellfqp geawkelqvk llelqevdsl lrgrqvrrfh 1081  vqlsnpkfga hlgqphstti iirdpdeldr sftsqmlssq ppphgdlgap qnpnakaags 1141  rkihfnwlpp sgkpmgyrvk ywiqgdsese ahlldskvps veltnlypyc dyemkvcayg 1201  aqgegpyssl vscrthqevp sepgrlafnv vsstvtqlsw aepaetngei tayevcyglv 1261  nddnrpigpm kkvlvdnpkn rmllienlre sqpyrytvka rngagwgper eaiinlatqp 1321  krpmsipiip dipivdaqsg edydsflmys ddvlrspsgs qrpsvsddte hlvngrmdfa 1381  fpgstnslhr mtttsaaayg thlsphvphr vlstsstltr dynsltrseh shsttlprdy 1441  stltsvsshd srltagvpdt ptrlvfsalg ptslrvswqe prcerplqgy sveyqllngg 1501  elhrlnipnp aqtsvvvedl lpnhsyvfrv raqsqegwgr eregvities qvhpqsplcp 1561  lpgsaftlst psapgplvft alspdslqls werprrpngd ivgylvtcem aqgggpataf 1621  rvdgdspesr ltvpglsenv pykfkvqart tegfgpereg iitiesqdgg pfpqlgsrag 1681  lfqhplqsey ssittthtsa tepflvdglt lgaqhleagg sltrhvtqef vsrtlttsgt 1741  lsthmdqqff qt Gene ID: Y22 Gene symbol: PVRL4 Gene description: Poliovirus receptor-related protein 4 Unigene: Hs.492490 Genbank: BC010423 Entrez Gene: 81607 Refseq: NM_030916 Protein sequence (SEQ ID NO (95)):    1  mplslgaemw gpeawlllll llasftgrcp ageletsdvv tvvlgqdakl pcfyrgdsge   61  qvgqvawarv dagegaqela llhskyglhv spayegrveq pppprnpldg svllrnavqa  121  degeyecrvs tfpagsfqar lrlrvlvppl pslnpgpale egqgltlaas ctaegspaps  181  vtwdtevkgt tssrsfkhsr saavtsefhl vpsrsmngqp ltcvvshpgl lqdqrithil  241  hvsflaeasv rgledqnlwh igregamlkc lsegqpppsy nwtrldgplp sgvrvdgdtl  301  gfpplttehs giyvchvsne fssrdsqvtv dvldpqedsg kqvdlvsasv vvvgviaall  361  fcllvvvvvl msryhrrkaq qmtqkyeeel tltrensirr lhshhtdprs qpeesvglra  421  eghpdslkdn sscsvmseep egrsystltt vreietqtel lspgsgraee eedqdegikq  481  amnhfvqeng tlrakptgng iyingrghlv Gene ID: Y23 Gene symbol: SDC1 Gene description: Syndecan-1 Unigene: Hs.224607 Genbank: BC008765 Entrez Gene: 6382 Refseq: NM_001006946 Protein sequence (SEQ ID NO (96)):    1  mrraalwlwl calalslqpa lpqivatnlp pedqdgsgdd sdnfsgsgag alqditlsqq   61  tpstwkdtql ltaiptspep tgleataast stlpagegpk egeavvlpev epgltareqe  121  atprprettq lptthqastt tattaqepat shphrdmqpg hhetstpagp sqadlhtpht  181  edggpsater aaedgassql paaegsgeqd ftfetsgent avvavepdrr nqspvdqgat  241  gasqglldrk evlggviagg lvglifavcl vgfmlyrmkk kdegsyslee pkqanggayq  301  kptkqeefya Gene ID: Z1 Gene symbol: ENPP1 Gene description: Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 Unigene: Hs.527295 Genbank: BC059375 Entrez Gene: 5167 Refseq: NM_006208 Protein sequence (SEQ ID NO (97)):    1  merdgcaggg srggeggrap regpagngrd rgrshaaeap gdpqaaasll apmdvgeepl   61  ekaarartak dpntykvlsl vlsvcvltti lgcifglkps cakevksckg rcfertfgnc  121  rcdaacvelg nccldyqetc iepehiwten kfrcgekrlt rslcacsddc kdkgdcciny  181  ssvcqgeksw veepcesine pqcpagfetp ptllfsldgf raeylhtwgg llpvisklkk  241  cgtytknmrp vyptktfpnh ysivtglype shgiidnkmy dpkmnasfsl kskekfnpew  301  ykgepiwvta kyqglksgtf fwpgsdvein gifpdiykmy ngsvpfeeri lavlqwlqlp  361  kderphfytl yleepdssgh sygpvssevi kalqrvdgmv gmlmdglkel nlhrclnlil  421  isdhgmeqgs ckkyiylnky lgdvknikvi ygpaarlrps dvpdkyysfn yegiarnlsc  481  repnqhfkpy lkhflpkrlh faksdriepl tfyldpqwql alnpserkyc gsgfhgsdnv  541  fsnmqalfvg ygpgfkhgie adtfenievy nlmcdllnlt papnngthgs lnhllknpvy  601  tpkhpkevhp lvqcpftrnp rdnlgcscnp silpiedfqt qfnltvaeek iikhetlpyg  661  rprvlqkent icllsqhqfm sgysqdilmp lwtsytvdrn dsfstedfsn clyqdfripl  721  spvhkcsfyk nntkvsygfl sppqlnknss giysealltt nivpmyqsfq viwryfhdtl  781  lrkyaeerng vnvvsgpvfd fdydgrcdsl enlrqkrrvi rnqeilipth ffivltsckd  841  tsqtplhcen ldtlafilph rtdnsescvh gkhdsswvee llmlhrarit dvehitglsf  901  yqqrkepvsd ilklkthlpt fsqed Gene ID: Z2 Gene symbol: CD34 Gene description: Hematopoietic progenitor cell antigen CD34 Unigene: Hs.374990 Genbank: M81104 Entrez Gene: 947 Refseq: NM_001773|NM_001025109 Protein sequence isoform b (SEQ ID NO (98)):    1  mlvrrgarag prmprgwtal cllsllpsgf msldnngtat pelptqgtfs nvstnvsyqe   61  tttpstlgst slhpvsqhgn eattnitett vkftstsvit svygntnssv qsqtsvistv  121  fttpanvstp ettlkpslsp gnvsdlstts tslatsptkp ytssspilsd ikaeikcsgi  181  revkltqgic leqnktssca efkkdrgegl arvlcgeeqa dadagaqvcs lllaqsevrp  241  qclllvlanr teissklqlm kkhqsdlkkl gildfteqdv ashqsysqkt lialvtsgal  301  lavlgitgyf lmnrrswspt gerlelep Protein sequence isoform a (SEQ ID NO (99)):    1  mlvrrgarag prmprgwtal cllsllpsgf msldnngtat pelptqgtfs nvstnvsyqe   61  tttpstlgst slhpvsqhgn eattnitett vkftstsvit svygntnssv qsqtsvistv  121  fttpanvstp ettlkpslsp gnvsdlstts tslatsptkp ytssspilsd ikaeikcsgi  181  revkltqgic leqnktssca efkkdrgegl arvlcgeeqa dadagaqvcs lllaqsevrp  241  qclllvlanr teissklqlm kkhqsdlkkl gildfteqdv ashqsysqkt lialvtsgal  301  lavlgitgyf lmnrrswspt gerlgedpyy tengggqgys sgpgtspeaq gkasvnrgaq  361  engtgqatsr nghsarqhvv adtel Gene ID: Z3 Gene symbol: JAM3 Gene description: Junctional adhesion molecule C Unigene: Hs.150718 Genbank: BC012147 Entrez Gene: 83700 Refseq: NM_032801|NM_001205329 Protein sequence isoform 1 (SEQ ID NO (100)):    1  malrrpprlr lcarlpdffl lllfrgclig avnlkssnrt pvvqefesve lsciitdsqt   61  sdpriewkki qdeqttyvff dnkiqgdlag raeilgktsl kiwnvtrrds alyrcevvar  121  ndrkeideiv ieltvqvkpv tpvcrvpkav pvgkmatlhc qeseghprph yswyrndvpl  181  ptdsranprf rnssfhlnse tgtlvftavh kddsgqyyci asndagsarc eeqemevydl  241  niggiiggvl vvlavlalit lgiccayrrg yfinnkqdge syknpgkpdg vnyirtdeeg  301  dfrhkssfvi Protein sequence isoform 2 (SEQ ID NO (101)):    1  malrrpprlr lcarlpdffl lllfrgclig avnlkssnrt pvvqefesve lsciitdsqt   61  sdpriewkki qdeqttyvff dnkiqvkpvt pvcrvpkavp vgkmatlhcq eseghprphy  121  swyrndvplp tdsranprfr nssfhlnset gtlvftavhk ddsgqyycia sndagsarce  181  eqemevydln iggiiggvlv vlavlalitl giccayrrgy finnkqdges yknpgkpdgv  241  nyirtdeegd frhkssfvi Gene ID: Z4 Gene symbol: CD14 Gene description: Monocyte differentiation antigen CD14 Unigene: Hs.163867 Genbank: BC010507 Entrez Gene: 929 Refseq: NM_000591 Protein sequence (SEQ ID NO (102)):    1  merascllll llplvhvsat tpepceldde dfrcvcnfse pqpdwseafq cvsaveveih   61  agglnlepfl krvdadadpr qyadtvkalr vrrltvgaaq vpaqllvgal rvlaysrlke  121  ltledlkitg tmpplpleat glalsslrlr nvswatgrsw laelqqwlkp glkvlsiaqa  181  hspafsceqv rafpaltsld lsdnpglger glmaalcphk fpaiqnlalr ntgmetptgv  241  caalaaagvq phsldlshns lratvnpsap rcmwssalns lnlsfagleq vpkglpaklr  301  vldlscnrln rapqpdelpe vdnltldgnp flvpgtalph egsmnsgvvp acarstlsvg  361  vsgtlvllqg argfa Gene ID: Z5 Gene symbol: PLSCR4 Gene description: Phospholipid scramblase 4 Unigene: Hs.477869 Genbank: AF199023 Entrez Gene: 57088 Refseq: NM_001128304|NM_001128306|NM_001177304 Protein sequence isoform a (SEQ ID NO (103)):    1  msgvvptape qpagemenqt kppdprpdap peynshflpg ppgtavpppt gypgglpmgy   61  yspqqpstfp lyqpvggihp vryqpgkypm pnqsvpitwm pgptpmancp pgleylvqld  121  nihvlqhfep lemmtcfetn nrydiknnsd qmvyivtedt ddftrnayrt lrpfvlrvtd  181  cmgreimtmq rpfrctcccf ccpsarqele vqcppgvtig fvaehwnlcr avysiqnekk  241  envmrvrgpc stygcgsdsv fevksldgis nigsiirkwn gllsamadad hfdihfpldl  301  dvkmkamifg acflidfmyf ersppqrsr Protein sequence isoform b (SEQ ID NO (104)):    1  msgvvptape qpagemenqt kppdprpdap peynshflpg ppgtavpppt gypgglpmgy   61  yspqqpstfp lyqpvggihp vryqpgkypm pnqsvpitwm pgptpmancp pgleylvqle  121  vqcppgvtig fvaehwnlcr avysiqnekk envmrvrgpc stygcgsdsv fevksldgis  181  nigsiirkwn gllsamadad hfdihfpldl dvkmkamifg acflidfmyf ersppqrsr Protein sequence isoform c (SEQ ID NO (105)):    1  menqtkppdp rpdappeyns hflpgppgta vppptgypgg lpmgyyspqq pstfplyqpv   61  ggihpvryqp gkypmpnqsv pitwmpgptp mancppgley lvqlevqcpp gvtigfvaeh  121  wnlcravysi qnekkenvmr vrgpcstygc gsdsvfevks ldgisnigsi irkwngllsa  181  madadhfdih fpldldvkmk amifgacfli dfmyferspp qrsr Gene ID: Z6 Gene symbol: AMOT Gene description: angiomotin Unigene: Hs.528051 Genbank: AF286598 Entrez Gene: 154796 Refseq: NM_133265|NM_001113490 Protein sequence isoform 2 (SEQ ID NO (106)):    1  mpraqpssas yqpvpadpfa ivsraqqmve ilsdenrnlr qelegcyekv arlqkvetei   61  qrvseayenl vkssskreal ekamrnkleg eirrmhdfnr direrletan kqlaekeyeg  121  sedtrktisq lfaknkesqr ekekleaela tarstnedqr rhieirdqal snaqakvvkl  181  eeelkkkqvy vdkvekmqqa lvqlqaacek reqlehrlrt rlereleslr iqqrqgncqp  241  tnvseynaaa lmellrekee rilaleadmt kweqkyleen vmrhfaldaa atvaaqrdtt  301  vishspntsy dtaleariqk eeeeilmank rcldmegrik tlhaqiiekd amikvlqqrs  361  rkepskteql scmrpakslm sisnagsgll shsstltgsp imeekrddks wkgslgillg  421  gdyraeyvps tpspvppstp llsahsktgs rdcstqterg tesnktaava pisvpapvaa  481  aataaaitat aatitttmva aapvavaaaa apaaaaapsp ataaataaav spaaagqipa  541  aasvasaaav apsaaaaaav qvapaapapv papalvpvpa paaaqasapa qtqaptsapa  601  vaptpaptpt pavaqaevpa spatgpgphr lsipsltcnp dktdgpvfhs ntlerktpiq  661  ilgqepdaem veyli Protein sequence isoform 1 (SEQ ID NO (107)):    1  mrnseeqpsg gttvlqrllq eqlrygnpse nrsllaihqq atgngppfps gsgnpgpqsd   61  vlspqdhhqq lvahaarqep qgqeiqsenl imekqlsprm qnneelptye eakvqsqyfr  121  gqqhasvgaa fyvtgvtnqk mrtegrpsvq rlnpgkmhqd eglrdlkqgh vrslserlmq  181  mslatsgvka hppvtsapls ppqpndlykn ptsssefyka qgplpnqhsl kgmehrgppp  241  eypfkgmppq svvckpqepg hfysehrlnq pgrtegqlmr yqhppeygaa rpaqdislpl  301  sarnsqphsp tssltsggsl pllqsppstr lsparhplvp nqgdhsahlp rpqqhflpnq  361  ahqgdhyrls qpglsqqqqq qqqqhhhhhh hqqqqqqqpq qqpgeaysam praqpssasy  421  qpvpadpfai vsraqqmvei lsdenrnlrq elegcyekva rlqkveteiq rvseayenlv  481  kssskreale kamrnklege irrmhdfnrd lrerletank qlaekeyegs edtrktisql  541  faknkesqre kekleaelat arstnedqrr hieirdqals naqakvvkle eelkkkqvyv  601  dkvekmqqal vqlqaacekr eqlehrlrtr lereleslri qqrqgncqpt nvseynaaal  661  mellrekeer ilaleadmtk weqkyleenv mrhfaldaaa tvaaqrdttv ishspntsyd  721  taleariqke eeeilmankr cldmegrikt lhaqiiekda mikvlqqrsr kepskteqls  781  cmrpakslms isnagsglls hsstltgspi meekrddksw kgslgillgg dyraeyvpst  841  pspvppstpl lsahsktgsr dcstqtergt esnktaavap isvpapvaaa ataaaitata  901  atitttmvaa apvavaaaaa paaaaapspa taaataaavs paaagqipaa asvasaaava  961  psaaaaaavq vapaapapvp apalvpvpap aaaqasapaq tqaptsapav aptpaptptp 1021  avaqaevpas patgpgphrl sipsltcnpd ktdgpvfhsn tlerktpiqi lgqepdaemv 1081  eyli Gene ID: Z7 Gene symbol: ENPEP Gene description: glutamyl aminopeptidase (aminopeptidase A) Unigene: Hs.435765 Genbank: L12468 Entrez Gene: 2028 Refseq: NM_001977 Protein sequence (SEQ ID NO (108)):    1  mnfaeregsk ryciqtkhva ilcavvvgvg livglavglt rscdssgdgg pgtapapshl   61  psstaspsgp paqdqdicpa sedesgqwkn frlpdfvnpv hydlhvkpll eedtytgtvs  121  isinlsaptr ylwlhlretr itrlpelkrp sgdqvqvrrc feykkqeyvv veaeeeltps  181  sgdglylltm efagwlngsl vgfyrttyte ngqvksivat dheptdarks fpcfdepnkk  241  atytisithp keygalsnmp vakeesvddk wtrttfeksv pmstylvcfa vhqfdsvkri  301  snsgkpltiy vqpeqkhtae yaanitksvf dyfeeyfamn yslpkldkia ipdfgtgame  361  nwglityret nllydpkesa ssnqqrvatv vahelvhqwf gnivtmdwwe dlwlnegfas  421  ffeflgvnha etdwqmrdqm lledvlpvqe ddslmsshpi ivtvttpdei tsvfdgisys  481  kgssilrmle dwikpenfqk gcqmylekyq fknaktsdfw aaleeasrlp vkevmdtwtr  541  qmgypvlnvn gvknitqkrf lldpranpsq ppsdlgytwn ipvkwtedni tssvlfnrse  601  kegitlnssn psgnaflkin pdhigfyrvn yevatwdsia talslnhktf ssadraslid  661  dafalaraql ldykvalnlt kylkreenfl pwqrvisavt yiismfeddk elypmieeyf  721  qgqvkpiads lgwndagdhv tkllrssvlg fackmgdrea lnnasslfeq wlngtvslpv  781  nlrllvyryg mqnsgneisw nytleqyqkt slaqekekll yglasvknvt llsryldllk  841  dtnliktqdv ftviryisyn sygknmawnw iqlnwdylvn rytlnnrnlg rivtiaepfn  901  telqlwqmes ffakypqaga gekpreqvle tvknniewlk qhrntirewf fnllesg Gene ID: Z8 Gene symbol: THY1 Gene description: Thy-1 cell surface antigen Unigene: Hs.644697 Genbank: AP003396 Entrez Gene: 7070 Refseq: NM_006288 Protein sequence (SEQ ID NO (109)):    1  mnlaisiall ltvlqvsrgq kvtsltaclv dqslrldcrh entssspiqy efsltretkk   61  hvlfgtvgvp ehtyrsrtnf tskynmkvly lsaftskdeg tytcalhhsg hsppissqnv  121  tvlrdklvkc egisllaqnt swllllllsl sllqatdfms l

Claims

1. A method of forming a composition enriched with human corneal endothelial cells comprising: (a) contacting a cell population containing human corneal cells with a first positive affinity reagent that selectively binds to human corneal endothelial cells relative to cells other than human corneal endothelial cells and (b) selecting cells to which the first positive affinity reagent is bound.

2. The method of claim 1, wherein the first positive affinity reagent selectively binds to human corneal endothelial cells relative to corneal keratocytes, human corneal endothelial cells of lower utility, or both.

3. The method of claim 1, wherein said first positive affinity reagent comprises an antibody or aptamer that binds to a corneal protein selected from protein products of genes X1 through X26 of Table 2.

4. The method of claim 1, wherein said first positive affinity reagent comprises an antibody or aptamer that binds to a protein product of gene X5 of Table 2, an antibody or aptamer that binds to a protein product of gene X15 of Table 2, or an antibody or aptamer that binds to a protein product of X25 of Table 2.

5. The method of claim 1, wherein said first positive affinity reagent comprises (a) an antibody or aptamer that binds to SEQ ID NO (8), (b) an antibody or aptamer that binds to one or more of SEQ ID NO (27), SEQ ID NO (28), SEQ ID NO (29), SEQ ID NO (30) or SEQ ID NO (31), or (c) an antibody or aptamer that binds to one or more of SEQ ID NO (53), SEQ ID NO (54), SEQ ID NO (55), SEQ ID NO (56) or SEQ ID NO (57).

6. The method of any of claims 1-5, wherein the first positive affinity reagent comprises an antibody or aptamer that is coupled to a solid matrix.

7. The method of any of claims 1-6, wherein the first positive affinity reagent comprises an antibody or aptamer that is coupled to a label.

8. The method of claim 7, wherein the label is selected from a magnetic label, a hapten (e.g., biotin) and a fluorescent label.

9. The method of any of claims 1-8, further comprising (a) contacting said cell population containing human corneal cells with a second positive affinity reagent that selectively binds to human corneal endothelial cells relative to cells other than human corneal endothelial cells and (b) selecting cells to which the second positive affinity reagent is bound, wherein the second positive affinity reagent differs from the first positive affinity reagent.

10. The method of claim 9, wherein said second positive affinity reagent comprises an antibody or aptamer that binds to a corneal protein selected from protein products of genes X1 through X26 of Table 2.

11. The method of claim 9, wherein said second positive affinity reagent comprises an antibody or aptamer that binds to a protein product of gene X5 of Table 2, an antibody or aptamer that binds to a protein product of gene X15 of Table 2, or an antibody or aptamer that binds to a protein product of X25 of Table 2.

12. The method of claim 9, wherein said second positive affinity reagent comprises (a) an antibody or aptamer that binds to SEQ ID NO (8), (b) an antibody or aptamer that binds to one or more of SEQ ID NO (27), SEQ ID NO (28), SEQ ID NO (29), SEQ ID NO (30) or SEQ ID NO (31), or (c) an antibody or aptamer that binds to one or more of SEQ ID NO (53), SEQ ID NO (54), SEQ ID NO (55), SEQ ID NO (56) or SEQ ID NO (57).

13. The method of any of claims 9-12, wherein the second positive affinity reagent comprises an antibody or aptamer that is coupled to a solid matrix or a label.

14. The method of any of claims 1-13, further comprising (a) contacting said cell population containing human corneal cells with a first negative affinity reagent that selectively binds to cells other than human corneal endothelial cells relative to human corneal endothelial cells and (b) removing the cells to which the first negative affinity reagent is bound.

15. The method of claim 14, wherein the first negative affinity reagent selectively binds to corneal keratocytes, human corneal endothelial cells of lower utility, or both, relative to human corneal endothelial cells.

16. The method of claim 14, wherein said first negative affinity reagent comprises an antibody or aptamer that binds to a corneal protein selected from protein products of genes Y1 through Y23 of Table 2 and protein products of genes Z1 through Z8 of Table 2.

17. The method of claim 14, wherein said first negative affinity reagent comprises an antibody or aptamer that binds to a protein product of gene Y6 of Table 2.

18. The method of claim 14, wherein said first negative affinity reagent comprises an antibody or aptamer that binds to one or more of SEQ ID NO (66), SEQ ID NO (67) or SEQ ID NO (68).

19. The method of any of claims 14-18, wherein the first negative affinity reagent comprises an antibody or aptamer that is coupled to a solid matrix or a label.

20. A composition enriched with human corneal endothelial cells that is made by the method of any of claims 1-19.

21. A kit comprising (a) a positive affinity reagent that selectively binds to human corneal endothelial cells relative to cells other than human corneal endothelial cells and (b) a negative affinity reagent that selectively binds to cells other than human corneal endothelial cells relative to human corneal endothelial cells.

22. The kit of claim 21, wherein said positive affinity reagent comprises an antibody or aptamer that binds to a corneal protein selected from protein products of genes X1 through X26 of Table 2.

23. The kit of claim 21, wherein said first positive affinity reagent comprises an antibody or aptamer that binds to a protein product of gene X5 of Table 2, an antibody or aptamer that binds to a protein product of gene X15 of Table 2, or an antibody or aptamer that binds to a protein product of X25 of Table 2.

24. The kit of claim 21, wherein said first positive affinity reagent comprises (a) an antibody or aptamer that binds to SEQ ID NO (8), (b) an antibody or aptamer that binds to one or more of SEQ ID NO (27), SEQ ID NO (28), SEQ ID NO (29), SEQ ID NO (30) or SEQ ID NO (31), or (c) an antibody or aptamer that binds to one or more of SEQ ID NO (53), SEQ ID NO (54), SEQ ID NO (55), SEQ ID NO (56) or SEQ ID NO (57).

25. The kit of any of claims 21-24, wherein the positive affinity reagent comprises an antibody or aptamer that is coupled to a solid matrix or a label.

26. The kit of any of claims 21-25, wherein said negative affinity reagent comprises an antibody or aptamer that binds to a corneal protein selected from protein products of genes Y1 through Y23 and protein products of genes Z1 through Z8 of Table 2.

27. The kit of any of claims 21-25, wherein said negative affinity reagent comprises an antibody or aptamer that binds to a protein product of gene Y6 of Table 2.

28. The kit of any of claims 21-25, wherein said first negative affinity reagent comprises an antibody or aptamer that binds to one or more of SEQ ID NO (66), SEQ ID NO (67) or SEQ ID NO (68).

29. The kit of any of claims 21-28, wherein the negative affinity reagent comprises an antibody or aptamer that is coupled to a solid matrix or a label.

30. An affinity reagent selected from (a) a solid matrix or a label coupled to an antibody or aptamer that binds to a corneal protein selected from protein products of genes X1 through X26 of Table 2 and (b) a solid matrix or a label coupled to an antibody or aptamer that binds to a corneal protein selected from protein products of genes Y1 through Y23 and protein products of genes Z1 through Z8 of Table 2.

31. A composition enriched with human corneal endothelial cells comprising: (a) human corneal cells and (b) a first positive affinity reagent that selectively binds to human corneal endothelial cells relative to cells other than human corneal endothelial cells.

32. The composition of claim 31, comprising human corneal endothelial cells to which the first positive affinity reagent is bound.

33. The composition of any of claims 31-32, wherein the first positive affinity reagent selectively binds to human corneal endothelial cells relative to corneal keratocytes, human corneal endothelial cells of lower utility, or both.

34. The composition of any of claims 31-33, wherein said first positive affinity reagent comprises an antibody or aptamer that binds to a corneal protein selected from protein products of genes X1 through X26 of Table 2.

35. The composition of any of claims 31-33, wherein said first positive affinity reagent comprises an antibody or aptamer that binds to a protein product of gene X5 of Table 2, an antibody or aptamer that binds to a protein product of gene X15 of Table 2, or an antibody or aptamer that binds to a protein product of X25 of Table 2.

36. The composition of any of claims 31-33, wherein said first positive affinity reagent comprises (a) an antibody or aptamer that binds to SEQ ID NO (8), (b) an antibody or aptamer that binds to one or more of SEQ ID NO (27), SEQ ID NO (28), SEQ ID NO (29), SEQ ID NO (30) or SEQ ID NO (31), or (c) an antibody or aptamer that binds to one or more of SEQ ID NO (53), SEQ ID NO (54), SEQ ID NO (55), SEQ ID NO (56) or SEQ ID NO (57).

37. The composition of any of claims 31-36, wherein the first positive affinity reagent comprises an antibody or aptamer that is coupled to a label.

38. The composition of claim 37, wherein the label is selected from a magnetic label, a hapten (e.g., biotin) and a fluorescent label.

39. The composition of any of claims 31-38, further comprising a second positive affinity reagent that selectively binds to human corneal endothelial cells relative to cells other than human corneal endothelial cells, wherein the second positive affinity reagent differs from the first positive affinity reagent.

40. The composition of claim 39, comprising human corneal endothelial cells to which the second positive affinity reagent is bound.

41. The composition of any of claims 39-40, wherein said second positive affinity reagent comprises an antibody or aptamer that binds to a corneal protein selected from protein products of genes X1 through X26 of Table 2.

42. The composition of any of claims 39-40, wherein said second positive affinity reagent comprises an antibody or aptamer that binds to a protein product of gene X5 of Table 2, an antibody or aptamer that binds to a protein product of gene X15 of Table 2, or an antibody or aptamer that binds to a protein product of X25 of Table 2.

43. The composition of any of claims 39-40, wherein said second positive affinity reagent comprises (a) an antibody or aptamer that binds to SEQ ID NO (8), (b) an antibody or aptamer that binds to one or more of SEQ ID NO (27), SEQ ID NO (28), SEQ ID NO (29), SEQ ID NO (30) or SEQ ID NO (31), or (c) an antibody or aptamer that binds to one or more of SEQ ID NO (53), SEQ ID NO (54), SEQ ID NO (55), SEQ ID NO (56) or SEQ ID NO (57).

44. The composition of any of claims 39-43, wherein the second positive affinity reagent comprises a label.

45. The composition of any of claims 31-44, comprising a measurable amount of one or more negative affinity reagents that selectively binds to cells other than human corneal endothelial cells relative to human corneal endothelial cells.

46. The composition of claim 45, wherein the one or more negative affinity reagents selectively binds to corneal keratocytes, human corneal endothelial cells of lower utility, or both, relative to human corneal endothelial cells.

47. The composition of any of claim 45-46, wherein said one or more negative affinity reagents comprise one or more antibodies that bind to one or more corneal proteins selected from protein products of genes Y1 through Y23 of Table 2 and protein products of genes Z1 through Z8 of Table 2.

48. The composition of any of claims 45-46, wherein said one or more negative affinity reagents comprise an antibody or aptamer that binds to a protein product of gene Y6 of Table 2.

49. The composition of any of claims 45-46, wherein said one or more negative affinity reagents comprise an antibody or aptamer that binds to one or more of SEQ ID NO (66), SEQ ID NO (67) or SEQ ID NO (68).

50. The composition of any of claims 45-49, wherein the one or more negative affinity reagents comprise an antibody or aptamer that is coupled to a label.

Patent History
Publication number: 20240027446
Type: Application
Filed: Jul 31, 2023
Publication Date: Jan 25, 2024
Inventors: Jeffrey L. Goldberg (Menlo Park, CA), Noelia J. Kunzevitzky (Menlo Park, CA)
Application Number: 18/362,678
Classifications
International Classification: G01N 33/569 (20060101); G01N 33/50 (20060101); C12N 5/079 (20060101);