Inbred maize line PH54B
An inbred maize line, designated PH54B, the plants and seeds of inbred maize line PH54B, methods for producing a maize plant produced by crossing the inbred line PH54B with itself or with another maize plant, and hybrid maize seeds and plants produced by crossing the inbred line PH54B with another maize line or plant.
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This invention is in the field of maize breeding, specifically relating to an inbred maize line designated PH54B.
BACKGROUND OF THE INVENTIONThe goal of plant breeding is to combine in a single variety or hybrid various desirable traits. For field crops, these traits may include resistance to diseases and insects, tolerance to heat and drought, reducing the time to crop maturity, greater yield, and better agronomic quality. With mechanical harvesting of many crops, uniformity of plant characteristics such as germination and stand establishment, growth rate, maturity, and plant and ear height, is important.
Field crops are bred through techniques that take advantage of the plant's method of pollination. A plant is self-pollinated if pollen from one flower is transferred to the same or another flower of the same plant. A plant is cross-pollinated if the pollen comes from a flower on a different plant.
Plants that have been self-pollinated and selected for type for many generations become homozygous at almost all gene loci and produce a uniform population of true breeding progeny. A cross between two different homozygous lines produces a uniform population of hybrid plants that may be heterozygous for many gene loci. A cross of two plants each heterozygous at a number of gene loci will produce a population of hybrid plants that differ genetically and will not be uniform.
Maize (zea mays L.), often referred to as corn in the United States, can be bred by both self-pollination and cross-pollination techniques. Maize has separate male and female flowers on the same plant, located on the tassel and the ear, respectively. Natural pollination occurs in maize when wind blows pollen from the tassels to the silks that protrude from the tops of the ears.
A reliable method of controlling male fertility in plants offers the opportunity for improved plant breeding. This is especially true for development of maize hybrids, which relies upon some sort of male sterility system. There are several options for controlling male fertility available to breeders, such as: manual or mechanical emasculation (or detasseling), cytoplasmic male sterility, genetic male sterility, gametocides and the like.
Hybrid maize seed is typically produced by a male sterility system incorporating manual or mechanical detasseling. Alternate strips of two inbred varieties of maize are planted in a field, and the pollen-bearing tassels are removed from one of the inbreds (female). Providing that there is sufficient isolation from sources of foreign maize pollen, the ears of the detasseled inbred will be fertilized only from the other inbred (male), and the resulting seed is therefore hybrid and will form hybrid plants.
The laborious, and occasionally unreliable, detasseling process can be avoided by using cytoplasmic male-sterile (CMS) inbreds. Plants of a CMS inbred are male sterile as a result of factors resulting from the cytoplasmic, as opposed to the nuclear, genome. Thus, this characteristic is inherited exclusively through the female parent in maize plants, since only the female provides cytoplasm to the fertilized seed. CMS plants are fertilized with pollen from another inbred that is not male-sterile. Pollen from the second inbred may or may not contribute genes that make the hybrid plants male-fertile. Usually seed from detasseled fertile maize and CMS produced seed of the same hybrid are blended to insure that adequate pollen loads are available for fertilization when the hybrid plants are grown.
There are several methods of conferring genetic male sterility available, such as multiple mutant genes at separate locations within the genome that confer male sterility, as disclosed in U.S. Pat. Nos. 4,654,465 and 4,727,219 to Brar et al. and chromosomal translocations as described by Patterson in U.S. Pat. Nos. 3,861,709 and 3,710,511. These and all patents referred to are incorporated by reference. In addition to these methods, Albertsen et al., of Pioneer Hi-Bred, U.S. patent application Ser. No. 07/848,433, have developed a system of nuclear male sterility which includes: identifying a gene which is critical to male fertility; silencing this native gene which is critical to male fertility; removing the native promoter from the essential male fertility gene and replacing it with an inducible promoter; inserting this genetically engineered gene back into the plant; and thus creating a plant that is male sterile because the inducible promoter is not "on" resulting in the male fertility gene not being transcribed. Fertility is restored by inducing, or turning "on", the promoter, which in turn allows the gene that confers male fertility to be transcribed.
There are many other methods of conferring genetic male sterility in the art, each with its own benefits and drawbacks. These methods use a variety of approaches such as delivering into the plant a gene encoding a cytotoxic substance associated with a male tissue specific promoter or an antisense system in which a gene critical to fertility is identified and an antisense to that gene is inserted in the plant (see: Fabinjanski, et al. EPO 89/3010153.8 publication no. 329,308 and PCT application PCT/CA90/00037 published as WO 90/08828).
Another system useful in controlling male sterility makes use of gametocides. Gametocides are not a genetic system, but rather a topical application of chemicals. These chemicals affect cells that are critical to male fertility. The application of these chemicals affects fertility in the plants only for the growing season in which the gametocide is applied (see Carlson, Glenn R., U.S. Pat. No. 4,936,904). Application of the gametocide, timing of the application and genotype specificity often limit the usefulness of the approach.
The use of male sterile inbreds is but one factor in the production of maize hybrids. The development of maize hybrids requires, in general, the development of homozygous inbred lines, the crossing of these lines, and the evaluation of the crosses. Pedigree breeding and recurrent selection breeding methods are used to develop inbred lines from breeding populations. Breeding programs combine the genetic backgrounds from two or more inbred lines or various other broad-based sources into breeding pools from which new inbred lines are developed by selfing and selection of desired phenotypes. The new inbreds are crossed with other inbred lines and the hybrids from these crosses are evaluated to determine which of those have commercial potential.
Pedigree breeding starts with the crossing of two genotypes, each of which may have one or more desirable characteristics that is lacking in the other or which complements the other. If the two original parents do not provide all the desired characteristics, other sources can be included in the breeding population. In the pedigree method, superior plants are selfed and selected in successive generations. In the succeeding generations the heterozygous condition gives way to homogeneous lines as a result of self-pollination and selection. Typically in the pedigree method of breeding five or more generations of selfing and selection is practiced: F.sub.1 .fwdarw.F.sub.2 ; F.sub.3 .fwdarw.F.sub.4 ; F.sub.4 .fwdarw.F.sub.5, etc.
Recurrent selection breeding, backcrossing for example, can be used to improve an inbred line. Backcrossing can be used to transfer a specific desirable trait from one inbred or source to an inbred that lacks that trait. This can be accomplished, for example, by first crossing a superior inbred (recurrent parent) to a donor inbred (non-recurrent parent), that carries the appropriate gene(s) for the trait in question. The progeny of this cross is then mated back to the superior recurrent parent followed by selection in the resultant progeny for the desired trait to be transferred from the non-recurrent parent. After five or more backcross generations with selection for the desired trait, the progeny will be heterozygous for loci controlling the characteristic being transferred, but will be like the superior parent for most or almost all other genes. The last backcross generation is then selfed to give pure breeding progeny for the gene(s) being transferred.
A single cross hybrid maize variety is the cross of two inbred lines, each of which has a genotype that complements the genotype of the other. The hybrid progeny of the first generation is designated F.sub.1. In the development of hybrids only the F.sub.1 hybrid plants are sought. Preferred F.sub.1 hybrids are more s vigorous than their inbred parents. This hybrid vigor, or heterosis, can be manifested in many polygenic traits, including increased vegetative growth and increased yield.
The development of a hybrid maize variety involves three steps: (1) the selection of plants from various germplasm pools for initial breeding crosses; (2) the selfing of the selected plants from the breeding crosses for several generations to produce a series of inbred lines, which, although different from each other, breed true and are highly uniform; and (3) crossing the selected inbred lines with different inbred lines to produce the hybrid progeny (F.sub.1). During the inbreeding process in maize, the vigor of the lines decreases. Vigor is restored when two different inbred lines are crossed to produce the hybrid progeny (F.sub.1). An important consequence of the homozygosity and homogeneity of the inbred lines is that the hybrid between any two inbreds will always be the same. Once the inbreds that give a superior hybrid have been identified, the hybrid seed can be reproduced indefinitely as long as the homogeneity of the inbred parents is maintained.
A single cross hybrid is produced when two inbred lines are crossed to produce the F.sub.1 progeny. A double cross hybrid is produced from four inbred lines crossed in pairs (A.times.B and C.times.D) and then the two F.sub.1 hybrids are crossed again (A.times.B).times.(C.times.D). Much of the hybrid vigor exhibited by F.sub.1 hybrids is lost in the next generation (F.sub.2). Consequently, seed from hybrid varieties is not used for planting stock.
Maize is an important and valuable field crop. Thus, a continuing goal of plant breeders is to develop high-yielding maize hybrids that are agronomically sound based on stable inbred lines. The reasons for this goal are obvious: to maximize the amount of grain produced with the inputs used and minimize susceptibility of the crop to environmental stresses. To accomplish this goal, the maize breeder must select and develop superior inbred parental lines for producing hybrids. This requires identification and selection of genetically unique individuals that occur in a segregating population. The segregating population is the result of a combination of crossover events plus the independent assortment of specific combinations of alleles at many gene loci that results in specific genotypes. Based on the number of segregating genes, the frequency of occurrence of an individual with a specific genotype is less than 1 in 10,000. Thus, even if the entire genotype of the parents has been characterized and the desired genotype is known, only a few if any individuals having the desired genotype may be found in a large F.sub.2 or S.sub.0 population. Typically, however, the genotype of neither the parents nor the desired genotype is known in any detail.
In addition to the preceding problem, it is not known how the genotype will react with the environment. This genotype by environment interaction is an important, yet unpredictable, factor in plant breeding. A breeder of ordinary skill in the art cannot predict the genotype, how that genotype will interact with various environments or the resulting phenotypes of the developing lines, except perhaps in a very broad and general fashion. A breeder of ordinary skill in the art would also be unable to recreate the same line twice from the very same original parents as the breeder is unable to direct how the genomes combine or how they will interact with the environmental conditions. This unpredictability results in the expenditure of large amounts of research resources in the development of a superior new maize inbred line.
Pioneer research station staff propose about 400 to 500 new inbreds each year from over 2,000,000 pollinations. Of those proposed new inbreds, less than 50 and more commonly less than 30 are actually selected for commercial use.
SUMMARY OF THE INVENTIONAccording to the invention, there is provided a novel inbred maize line, designated PH54B. This invention thus relates to the seeds of inbred maize line PH54B, to the plants of inbred maize line PH54B, and to methods for producing a maize plant produced by crossing inbred maize line PH54B with itself or another maize line. This invention further relates to hybrid maize seeds and plants produced by crossing the inbred line PH54B with another maize line.
DefinitionsIn the description and examples that follow, a number of terms are used herein. In order to provide a clear and consistent understanding of the specification and claims, including the scope to be given such terms, the following definitions are provided. NOTE: ABS is in absolute terms and % MN is percent of the mean for the experiments in which the inbred or hybrid was grown. These designators will follow the descriptors to denote how the values are to be interpreted. Below are the descriptors used in the data tables included herein.
ANT ROT=ANTHRACNOSE STALK ROT (Colletotrichum graminicola). A 1 to 9 visual rating indicating the resistance to Anthracnose Stalk Rot. A higher score indicates a higher resistance.
BAR PLT=BARREN PLANTS. The percent of plants per plot that were not barren (lack ears).
BRT STK=BRITTLE STALKS. This is a measure of the stalk breakage near the time of pollination, and is an indication of whether a hybrid or inbred would snap or break near the time of flowering under severe winds. Data are presented as percentage of plants that did not snap.
BU ACR=YIELD (BUSHELS/ACRE). Yield of the grain at harvest in bushels per acre adjusted to 15.5% moisture.
CLN=CORN LETHAL NECROSIS. Synergistic interaction of maize chlorotic mottle virus (MCMV) in combination with either maize dwarf mosaic virus (MDMV-A or MDMV-B) or wheat streak mosaic virus (WSMV). A 1 to 9 visual rating indicating the resistance to Corn Lethal Necrosis. A higher score indicates a higher resistance.
COM RST=COMMON RUST (Puccinia sorghi). A 1 to 9 visual rating indicating the resistance to Common Rust. A higher score indicates a higher resistance.
D/D=DRYDOWN. This represents the relative rate at which a hybrid will reach acceptable harvest moisture compared to other hybrids on a 1-9 rating scale. A high score indicates a hybrid that dries relatively fast while a low score indicates a hybrid that dries slowly.
DIP ERS=DIPLODIA EAR MOLD SCORES (Diplodia maydis and Diplodia macroscpora). A 1 to 9 visual rating indicating the resistance to Diplodia Ear Mold. A higher score indicates a higher resistance.
DRP EAR=DROPPED EARS. A measure of the number of dropped ears per plot and represents the percentage of plants that did not drop ears prior to harvest.
D/T=DROUGHT TOLERANCE. This represents a 1-9 rating for drought tolerance, and is based on data obtained under stress conditions. A high score indicates good drought tolerance and a low score indicates poor drought tolerance.
EAR HT=EAR HEIGHT. The ear height is a measure from the ground to the highest placed developed ear node attachment and is measured in inches.
EAR MLD=General Ear Mold. Visual rating (1-9 score) where a "1" is very susceptible and a "9" is very resistant. This is based on overall rating for ear mold of mature ears without determining the specific mold organism, and may not be predictive for a specific ear mold.
EAR SZ=EAR SIZE. A 1 to 9 visual rating of ear size. The higher the rating the larger the ear size.
ECB 1LF=EUROPEAN CORN BORER FIRST GENERATION LEAF FEEDING (Ostrinia nubilalis). A 1 to 9 visual rating indicating the resistance to preflowering leaf feeding by first generation European Corn Borer. A higher score indicates a higher resistance.
ECB 2IT=EUROPEAN CORN BORER SECOND GENERATION INCHES OF TUNNELING (Ostrinia nubilalis). Average inches of tunneling per plant in the stalk.
ECB 2SC=EUROPEAN CORN BORER SECOND GENERATION (Ostrinia nubilalis). A 1 to 9 visual rating indicating post flowering degree of stalk breakage and other evidence of feeding by European Corn Borer, Second Generation. A higher score indicates a higher resistance.
ECB DPE=EUROPEAN CORN BORER DROPPED EARS (Ostrinia nubilalis). Dropped ears due to European Corn Borer. Percentage of plants that did not drop ears under second generation corn borer infestation.
EST CNT=EARLY STAND COUNT. This is a measure of the stand establishment in the spring and represents the number of plants that emerge on per plot basis for the inbred or hybrid.
EYE SPT=Eye Spot (Kabatiella zeae or Aureobasidium zeae). A 1 to 9 visual rating indicating the resistance to Eye Spot. A higher score indicates a higher resistance.
FUS ERS=FUSARIUM EAR ROT SCORE (Fusarium moniliforme or Fusarium subglutinans). A 1 to 9 visual rating indicating the resistance to Fusarium ear rot. A higher score indicates a higher resistance.
GDU=Growing Degree Units. Using the Barger Heat Unit Theory, which assumes that maize growth occurs in the temperature range 50.degree. F.-86.degree. F. and that temperatures outside this range slow down growth; the maximum daily heat unit accumulation is 36 and the minimum daily heat unit accumulation is 0. The seasonal accumulation of GDU is a major factor in determining maturity zones.
GDU SHD=GDU TO SHED. The number of growing degree units (GDUs) or heat units required for an inbred line or hybrid to have approximately 50 percent of the plants shedding pollen and is measured from the time of planting. Growing degree units are calculated by the Barger Method, where the heat units for a 24-hour period are: ##EQU1##
The highest maximum temperature used is 86.degree. F. and the lowest minimum temperature used is 50.degree. F. For each inbred or hybrid it takes a certain number of GDUs to reach various stages of plant development.
GDU SLK=GDU TO SILK. The number of growing degree units required for an inbred line or hybrid to have approximately 50 percent of the plants with silk emergence from time of planting. Growing degree units are calculated by the Barger Method as given in GDU SHD definition.
GIB ERS=GIBBERELLA EAR ROT (PINK MOLD) (Gibberella zeae). A 1 to 9 visual rating indicating the resistance to Gibberella Ear Rot. A higher score indicates a higher resistance.
GLF SPT=Gray Leaf Spot (Cercospora zeae-maydis). A 1 to 9 visual rating indicating the resistance to Gray Leaf Spot. A higher score indicates a higher resistance.
GOS WLT=Goss' Wilt (Corynebacterium nebraskense). A 1 to 9 visual rating indicating the resistance to Goss' Wilt. A higher score indicates a higher resistance.
GRN APP=GRAIN APPEARANCE. This is a 1 to 9 rating for the general appearance of the shelled grain as it is harvested based on such factors as the color of harvested grain, any mold on the grain, and any cracked grain. High scores indicate good grain quality.
H/POP=YIELD AT HIGH DENSITY. Yield ability at relatively high plant densities on 1-9 relative rating system with a higher number indicating the hybrid responds well to high plant densities for yield relative to other hybrids. A 1, 5, and 9 would represent very poor, average, and very good yield response, respectively, to increased plant density.
HC BLT=HELMINTHOSPORIUM CARBONUM LEAF BLIGHT (Helminthosporium carbonum). A 1 to 9 visual rating indicating the resistance to Helminthosporium infection. A higher score indicates a higher resistance.
HD SMT=HEAD SMUT (Sphacelotheca reiliana). This score indicates the percentage of plants not infected.
INC D/A=GROSS INCOME (DOLLARS PER ACRE). Relative income per acre assuming drying costs of two cents per point above 15.5 percent harvest moisture and current market price per bushel.
INCOME/ACRE. Income advantage of hybrid to be patented over other hybrid on per acre basis.
INC ADV=GROSS INCOME ADVANTAGE. GROSS INCOME advantage of variety #1 over variety #2.
L/POP=YIELD AT LOW DENSITY. Yield ability at relatively low plant densities on a 1-9 relative system with a higher number indicating the hybrid responds well to low plant densities for yield relative to other hybrids. A 1, 5, and 9 would represent very poor, average, and very good yield response, respectively, to low plant density.
MDM CPX=MAIZE DWARF MOSAIC COMPLEX (MDMV=Maize Dwarf Mosaic Virus and MCDV=Maize Chlorotic Dwarf Virus). A 1 to 9 visual rating indicating the resistance to Maize Dwarf Mosaic Complex. A higher score indicates a higher resistance.
MST=HARVEST MOISTURE. The moisture is the actual percentage moisture of the grain at harvest.
MST ADV=MOISTURE ADVANTAGE. The moisture advantage of variety #1 over variety #2 as calculated by: MOISTURE of variety #2-MOISTURE of variety #1 =MOISTURE ADVANTAGE of variety #1.
NLF BLT=Northern Leaf Blight (Helminthosporium turcicum or Exserohilum turcicum). A 1 to 9 visual rating indicating the resistance to Northern Leaf Blight. A higher score indicates a higher resistance.
PLT HT=PLANT HEIGHT. This is a measure of the height of the plant from the ground to the tip of the tassel in inches.
POL SC=POLLEN SCORE. A 1 to 9 visual rating indicating the amount of pollen shed. The higher the score the more pollen shed.
POL WT=POLLEN WEIGHT. This is calculated by dry weight of tassels collected as shedding commences minus dry weight from similar tassels harvested after shedding is complete.
It should be understood that the inbred can, through routine manipulation of cytoplasmic or other factors, be produced in a male-sterile form. Such embodiments are also contemplated within the scope of the present claims.
POP K/A=PLANT POPULATIONS. Measured as 1000s per acre.
POP ADV=PLANT POPULATION ADVANTAGE. The plant population advantage of variety #1 over variety #2 as calculated by PLANT POPULATION of variety #2-PLANT POPULATION of variety #1 =PLANT POPULATION ADVANTAGE of variety #1.
PRM=PREDICTED RELATIVE MATURITY. This trait, predicted relative maturity, is based on the harvest moisture of the grain. The relative maturity rating is based on a known set of checks and utilizes standard linear regression analyses and is also referred to as the Comparative Relative Maturity Rating System that is similar to the Minnesota Relative Maturity Rating System.
PRM SHD=A relative measure of the growing degree units (GDU) required to reach 50% pollen shed. Relative values are predicted values from the linear regression of observed GDU's on relative maturity of commercial checks.
RT LDG=ROOT LODGING. Root lodging is the percentage of plants that do not root lodge; plants that lean from the vertical axis at an approximately 30.degree. angle or greater would be counted as root lodged.
RTL ADV=ROOT LODGING ADVANTAGE. The root lodging advantage of variety #1 over variety #2.
SCT GRN=SCATTER GRAIN. A 1 to 9 visual rating indicating the amount of scatter grain (lack of pollination or kernel abortion) on the ear. The higher the score the less scatter grain.
SDG VGR=SEEDLING VIGOR. This is the visual rating (1 to 9) of the amount of vegetative growth after emergence at the seedling stage (approximately five leaves). A higher score indicates better vigor.
SEL IND=SELECTION INDEX. The selection index gives a single measure of the hybrid's worth based on information for up to five traits. A maize breeder may utilize his or her own set of traits for the selection index. One of the traits that is almost always included is yield. The selection index data presented in the tables represent the mean value averaged across testing stations.
SLF BLT=SOUTHERN LEAF BLIGHT (Helminthosporium maydis or Bipolaris maydis). A 1 to 9 visual rating indicating the resistance to Southern Leaf Blight. A higher score indicates a higher resistance.
SOU RST=SOUTHERN RUST (Puccinia polysora). A 1 to 9 visual rating indicating the resistance to Southern Rust. A higher score indicates a higher resistance.
STA GRN=STAY GREEN. Stay green is the measure of plant health near the time of black layer formation (physiological maturity). A high score indicates better late-season plant health.
STD ADV=STALK STANDING ADVANTAGE. The advantage of variety #1 over variety #2 for the trait STK CNT.
STK CNT=NUMBER OF PLANTS. This is the final stand or number of plants per plot.
STK LDG=STALK LODGING. This is the percentage of plants that did not stalk lodge (stalk breakage) as measured by either natural lodging or pushing the stalks and determining the percentage of plants that break below the ear.
STW WLT=Stewart's Wilt (Erwinia stewartii). A 1 to 9 visual rating indicating the resistance to Stewart's Wilt. A higher score indicates a higher resistance.
TAS BLS=TASSEL BLAST. A 1 to 9 visual rating was used to measure the degree of blasting (necrosis due to heat stress) of the tassel at the time of flowering. A 1 would indicate a very high level of blasting at time of flowering, while a 9 would have no tassel blasting.
TAS SZ=TASSEL SIZE. A 1 to 9 visual rating was used to indicate the relative size of the tassel. The higher the rating the larger the tassel.
TAS WT=TASSEL WEIGHT. This is the average weight of a tassel (grams) just prior to pollen shed.
TEX EAR=EAR TEXTURE. A 1 to 9 visual rating was used to indicate the relative hardness (smoothness of crown) of mature grain. A 1 would be very soft (extreme dent) while a 9 would be very hard (flinty or very smooth crown).
TILLER=TILLERS. A count of the number of tillers per plot that could possibly shed pollen was taken. Data is given as a percentage of tillers: number of tillers per plot divided by number of plants per plot.
TST WT=TEST WEIGHT (UNADJUSTED). The measure of the weight of the grain in pounds for a given volume (bushel).
TST WTA=TEST WEIGHT ADJUSTED. The measure of the weight of the grain in pounds for a given volume (bushel) adjusted for 15.5 percent moisture.
TSW ADV=TEST WEIGHT ADVANTAGE. The test weight advantage of variety #1 over variety #2.
WIN M%=PERCENT MOISTURE WINS.
WIN Y%=PERCENT YIELD WINS.
YLD=YIELD. It is the same as BU ACR ABS.
YLD ADV=YIELD ADVANTAGE. The yield advantage of variety #1 over variety #2 as calculated by: YIELD of variety #1=YIELD variety #2 =yield advantage of variety #1.
YLD SC=YIELD SCORE. A 1 to 9 visual rating was used to give a relative rating for yield based on plot ear piles. The higher the rating the greater visual yield appearance.
DETAILED DESCRIPTION OF THE INVENTIONInbred maize lines are typically developed for use in the production of hybrid maize lines. Inbred maize lines need to be highly homogeneous, homozygous and reproducible to be useful as parents of commercial hybrids. There are many analytical methods available to determine the homozygotic and phenotypic stability of these inbred lines.
The oldest and most traditional method of analysis is the observation of phenotypic traits. The data is usually collected in field experiments over the life of the maize plants to be examined. Phenotypic characteristics most often observed are for traits associated with plant morphology, ear and kernel morphology, insect and disease resistance, maturity, and yield.
In addition to phenotypic observations, the genotype of a plant can also be examined. There are many laboratory-based techniques available for the analysis, comparison and characterization of plant genotype; among these are Isozyme Electrophoresis, Restriction Fragment Length Polymorphisms (RFLPs), Randomly Amplified Polymorphic DNAs (RAPDs), Arbitrarily Primed Polymerase Chain Reaction (AP-PCR), DNA Amplification Fingerprinting (DAF), Sequence Characterized Amplified Regions (SCARs), Amplified Fragment Length Polymorphisms (AFLPs), and Simple Sequence Repeats (SSRs) which are also referred to as Microsatellites.
The most widely used of these laboratory techniques are Isozyme Electrophoresis and RFLPs as discussed in Lee, M., "Inbred Lines of Maize and Their Molecular Markers," The Maize Handbook, (Springer-Verlag, New York, Inc. 1994, at 423-432)incorporated herein by reference. Isozyme Electrophoresis is a useful tool in determining genetic composition, although it has relatively low number of available markers and the low number of allelic variants among maize inbreds. RFLPs have the advantage of revealing an exceptionally high degree of allelic variation in maize and the number of available markers is almost limitless.
Maize RFLP linkage maps have been rapidly constructed and widely implemented in genetic studies. One such study is described in Boppenmaier, et al., "Comparisons among strains of inbreds for RFLPs", Maize Genetics Cooperative Newsletter, 65:1991, pg. 90, is incorporated herein by reference. This study used 101 RFLP markers to analyze the patterns of 2 to 3 different deposits each of five different inbred lines. The inbred lines had been selfed from 9 to 12 times before being adopted into 2 to 3 different breeding programs. It was results from these 2 to 3 different breeding programs that supplied the different deposits for analysis. These five lines were maintained in the separate breeding programs by selfing or sibbing and rogueing off-type plants for an additional one to eight generations. After the RFLP analysis was completed, it was determined the five lines showed 0-2% residual heterozygosity. Although this was a relatively small study, it can be seen using RFLPs that the lines had been highly homozygous prior to the separate strain maintenance.
Inbred maize line PH54B is a yellow, dent maize inbred that is best suited as a male in crosses for producing first generation F.sub.1 maize hybrids. Inbred maize line PH54B is best adapted to the Northeast region of the United States and portions of Canada and can be used to produce hybrids at approximately 79-85 relative maturity based on the Comparative Relative Maturity Rating System for harvest moisture of grain. Inbred maize line PH54B yields extremely well for its maturity with fast dry down. This inbred has phenomenal yield to moisture ratio as demonstrated in the Tables included herewith and summarized below.
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TABLE BU ACR % MN MST % MN
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4A 106 95
4B 106 95
4C 106 96
4D 114 98
4E 111 99
4F 108 95
5A 111 95
5B 110 93
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The less favorable points of inbred PH54B are weak roots and low test weight, however, the high yield coupled with fast drydown make PH54B hybrids profitable to farmers in the earliest maturities of the North America Corn Belt. The taller plant and higher ear stature of PH54B are important additions to the PH54B hybrids as these allow PH54B hybrids to be used as both grain and silage hybrids.
The inbred has shown uniformity and stability within the limits of environmental influence for all the traits as described in the Variety Description Information (Table 1) that follows. The inbred has been self-pollinated and ear-rowed a sufficient number of generations with careful attention paid to uniformity of plant type to ensure the homozygosity and phenotypic stability necessary to use in commercial production. The line has been increased both by hand and in isolated fields with continued observation for uniformity. No variant traits have been observed or are expected in PH54B.
Inbred maize line PH54B, being substantially homozygous, can be reproduced by planting seeds of the line, growing the resulting maize plants under self-pollinating or sib-pollinating conditions with adequate isolation, and harvesting the resulting seed, using techniques familiar to the agricultural arts.
TABLE 1
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VARIETY DESCRIPTION INFORMATION
INBRED = PH54B
Type: Dent Region Best Adapted: Northeast
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A. Maturity: Average across maturity zones. Zone: 0
GDU Shed: 1190
GDU Silk: 1220
No. Reps: 31
B. Plant Characteristics:
Plant height (to tassel tip): 87 in.
C. Tassel:
Pollen Shed: Light based on Pollen Yield Test
(74% of experiment means)
Anther Color: Yellow*
Glume Color: Green*
D. Ear (Husked Ear Data Except When Stated Otherwise):
Silk Color: Green*
Husk Extension (Harvest stage): Medium (barely
covering ear)
Husk Leaf: Short
Taper of Ear: Average
Position of Shank (dry husks): Upright
Kernel Rows: Slightly cureved, indistinct
Husk Color (fresh): Light Green*
Husk Color (dry): Buff*
E. Cob:
Color: Red*
F. Diseases:
Carbonum Leaf Blight (H. carbonum): Resistant
N. Leaf Blight (E. turcicum): Susceptible
Eye Spot (K. zeae): Susceptible
Goss's Wilt (C. nebraskense): Resistant
Fusarium Ear Mold (F. moniliforme): Intermediate
Gibberella Ear Rot (G. zeae): Resistant
G. Insects:
European Corn Borer-1 Leaf Damage
(Pre-flowering): Susceptible
European Corn Borer-2 (Post-flowering): Resistant
The above descriptions are based on a scale of 1-9,
1 being highly susceptible, 9 being highly resistant
S (Susceptible): Would generally represent a score
of 1-3.
I (Intermediate): Would generally represent a score
of 4-5.
R (Resistant): Would generally represent a score of 6-7.
H (Highly Resistant): Would generally represent a
score of 8-9. Highly resistant does not imply
the inbred is immune.
H. Variety Most Closely Resembling:
Character: Inbred
Maturity PHGW7
Usage PHGW7
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*In interpreting the foregoing color designations, reference may be had t
the Munsell Glossy Book of Color, a standard color reference.
Data for Items B, C, D and E is based primarily on description informatio
from the maintaining station.
PHGW7 (PVP Certificate No. 9200083) is a Pioneer HiBred International,
Inc. proprietary inbred.
Industrial Applicability
This invention also is directed to methods for producing a maize plant by crossing a first parent maize plant with a second parent maize plant wherein the first or second parent maize plant is an inbred maize plant of the line PH54B. Further, both first and second parent maize plants can come from the inbred maize line PH54B. Thus, any such methods using the inbred maize line PH54B are part of this invention: selfing, backcrosses, hybrid production, crosses to populations, and the like. All plants produced using inbred maize line PH54B as a parent are within the scope of this invention. Advantageously, the inbred maize line is used in crosses with other, different, maize inbreds to produce first generation (F.sub.1) maize hybrid seeds and plants with superior characteristics.
As used herein, the term plant includes plant cells, plant protoplasts, plant cell tissue cultures from which maize plants can be regenerated, plant calli, plant clumps, and plant cells that are intact in plants or parts of plants, such as embryos, pollen, ovules, flowers, kernels, ears, cobs, leaves, husks, stalks, roots, root tips, anthers, silk and the like.
Duncan, Williams, Zehr, and Widholm, Planta (1985) 165:322-332 reflects that 97% of the plants cultured that produced callus were capable of plant regeneration. Subsequent experiments with both inbreds and hybrids produced 91% regenerable callus that produced plants. In a further study in 1988, Songstad, Duncan & Widholm in Plant Cell Reports (1988), 7:262-265 reports several media additions that enhance regenerability of callus of two inbred lines. Other published reports also indicated that "nontraditional" tissues are capable of producing somatic embryogenesis and plant regeneration. K. P. Rao, et al., Maize Genetics Cooperation Newsletter, 60:64-65 (1986), refers to somatic embryogenesis from glume callus cultures and B. V. Conger, et al., Plant Cell Reports, 6:345-347 (1987) indicates somatic embryogenesis from the tissue cultures of maize leaf segments. Thus, it is clear from the literature that the state of the art is such that these methods of obtaining plants are, and were, "conventional" in the sense that they are routinely used and have a very high rate of success.
Tissue culture of maize is described in European Patent Application, publication 160,390, incorporated herein by reference. Maize tissue culture procedures are also described in Green and Rhodes, "Plant Regeneration in Tissue Culture of Maize," Maize for Biological Research (Plant Molecular Biology Association, Charlottesville, Va. 1982, at 367-372) and in Duncan, et al., "The Production of Callus Capable of Plant Regeneration from Immature Embryos of Numerous Zea Mays Genotypes," 165 Planta 322-332 (1985). Thus, another aspect of this invention is to provide cells which upon growth and differentiation produce maize plants having the physiological and morphological characteristics of inbred line PH54B.
Maize is used as human food, livestock feed, and as raw material in industry. The food uses of maize, in addition to human consumption of maize kernels, include both products of dry- and wet-milling industries. The principal products of maize dry milling are grits, meal and flour. The maize wet-milling industry can provide maize starch, maize syrups, and dextrose for food use. Maize oil is recovered from maize germ, which is a by-product of both dry- and wet-milling industries.
Maize, including both grain and non-grain portions of the plant, is also used extensively as livestock feed, primarily for beef cattle, dairy cattle, hogs, and poultry.
Industrial uses of maize include production of ethanol, maize starch in the wet-milling industry and maize flour in the dry-milling industry. The industrial applications of maize starch and flour are based on functional properties, such as viscosity, film formation, adhesive properties, and ability to suspend particles. The maize starch and flour have application in the paper and textile industries. Other industrial uses include applications in adhesives, building materials, foundry binders, laundry starches, explosives, oil-well muds, and other mining applications.
Plant parts other than the grain of maize are also used in industry: for example, stalks and husks are made into paper and wallboard and cobs are used for fuel and to make charcoal.
The seed of inbred maize line PH54B, the plant produced from the inbred seed, the hybrid maize plant produced from the crossing of the inbred, hybrid seed, and various parts of the hybrid maize plant can be utilized for human food, livestock feed, and as a raw material in industry.
Performance Examples of PH54B
In the examples that follow, the traits and characteristics of inbred maize line PH54B are given as a line. The data collected on inbred maize line PH54B is presented for the key characteristics and traits.
The inbred per se results in Table 2A compare PH54B to its parent, PHR25. The results show PH54B is higher yielding than PHR25. Inbred PH54B has a significantly higher seedling vigor and early stand count than PHR25. In addition, PH54B is significantly taller with a significantly earlier GDU to shed and GDU to silk than PHR25. These traits allow PH54B hybrids to be grown in areas in the extreme north part of the Corn Belt, where cold soils and a short growing season limit a farmers choice of hybrids to vigorous hybrids that flower and mature quickly.
Table 2B compares PH54B to its other parent, PHN80. The inbred per se results show PH54B is higher yielding than PHN80. Inbred PH54B has a significantly higher seedling vigor and early stand count than PHN80. In addition, PH54B has significantly taller plants with higher ear height than PHN80.
The inbred per se results in Table 2C compare inbred PH54B to inbred PHK05. Again, PH54B is the higher yielding inbred. Although both inbreds have similar seedling vigor scores, inbred PH54B has a significantly higher early stand count and a significantly lower percentage of tillers. In addition, PH54B has significantly taller plants with higher ear height than PHK05.
The inbred per se results of Table 2D compare PH54B to PHT73. Although no yield data was reported in this comparison, inbred PH54B demonstrates a higher early stand count and a slightly higher seedling vigor. In addition, PH54B has taller plants with higher ears than PHT73.
Table 2E shows a comparison of inbred per se results of inbred PH54B and inbred PHG68. Although no yield data was reported in this comparison, inbred PH54B shows a significantly higher seedling vigor and early stand count than inbred PHG68. Inbred PH54B has significantly taller plants with higher ear placement than inbred PHG68.
The inbred per se results in Table 2F compare PH54B to PHM81. Inbred PH54B shows a somewhat higher yield with a lower test weight than inbred PHM81. As observed in previous tables, PH54B demonstrates a significantly higher seedling vigor and early stand count. In addition, PH54B has taller plants with higher ear placement than PHM81.
Tables 3A and 3B show a comparison of general combining ability of PH54B and other inbreds. Table 3A shows a comparison of PH54B to PHR25. In Table 3A, PH54B demonstrates a higher yield than PHR25.
The results of Table 3B compare the general combining ability of inbred PH54B to inbred PHK05. The hybrids of PH54B demonstrate a higher yield and faster drydown than the hybrids of PHK05. The PH54B hybrids show a higher resistance to root lodging and somewhat better resistance to stalk lodging than the PHK05 hybrids.
Tables 4A through 4F show comparisons of specific combining ability. Table 4A shows a PH54B hybrid, produced by crossing a PH54B hybrid, produced by crossing PH54B and PHRE1, and a PHAJ0 hybrid, produced by crossing PHAJ0 and PHRE 1. The PH54B hybrid has a significantly higher yield and better stalk lodging resistance than the PHAJ0 hybrid. The PH54B hybrid shows taller plants with higher ears than the PHAJ0 hybrid.
The results in Table 4B compare the specific combining ability of PH54B and PH90A. The PH54B hybrid is produced by crossing PH54B and PHRE1. The PH90A hybrid is produced by crossing PH90A and PHRE1. The PH54B hybrid has a significantly higher yield and faster drydown than the PH90A hybrid. The PH54B hybrid has taller plants with higher ear placement and higher resistance to stalk lodging than the PH90A hybrid.
Table 4C compares the specific combining ability of PH54B and PHKM5. The PH54B hybrid is produced by crossing PH54B and PHRE1. The PHKM5 hybrid is produced by crossing PHKM5 and PHRE1. The two hybrids show a similar yield and drydown while the PHKM5 hybrid demonstrates a higher test weight and seedling vigor. The PH54B hybrid has taller plants with higher ears than the PHKM5 hybrid.
Table 4D shows a comparison of the specific combining abilities of PH54B and PHT73. The PH54B hybrid is produced by crossing PH54B and PHFA5. The PHT73 hybrid is produced by crossing PHT73 and PHFA5. The PH54B hybrid is significantly higher yielding with significantly better stand establishment than the PHT73 hybrid. In addition, the PH54B hybrid has taller plants with higher ears and higher stay green score than the PHT73 hybrid.
Table 4E compares specific combining ability of PH54B and PHG68. The PH54B hybrid is produced by crossing PH54B and PHFA5. The PHG68 hybrid is produced by crossing PHG68 and PHFA5. The PH54B hybrid has a significantly higher yield than the PHG68 hybrid. The results also show the PH54B hybrid to have a higher early stand count as well as significantly better staygreen and resistance to stalk lodging than the PHG68 hybrid.
Table 4F compares the specific combining ability of PH54B and PHK05. The PH54B hybrid is produced by crossing PH54B and PHRE1. The PHK05 hybrid is produced by crossing PHK05 and PHRE1. The PH54B hybrid has significantly higher yield as well as Stalk Lodging resistance than the PHK05 hybrid.
Tables 5A and 5B show comparisons of PH54B hybrids and other hybrids of a similar maturity which have yield and standability deficiencies. The PH54B hybrids provide an improvement over these hybrids as seen in Tables 5A and 5B.
Table 5A shows the comparison of PH54B crossed to PHRE 1 and PHH51 crossed to PHK05. The PHH51/PHK05 hybrid is one of the earliest hybrids sold in the U.S. Corn Belt. The PH54B/PHRE 1 is 3 days later, but yields significantly more than the PHH51/PHK05 hybrid. The results also show improved roots and significantly better stalk lodging resistance.
Table 5B shows the comparison of PH54B crossed to PHRE 1 and PHJ40 crossed to PHT73. The PH54B/PHRE 1 hybrid has a 3 day earlier PRM and is significantly higher yielding with faster drydown than the PHJ40/PHT73 hybrid. Additionally, the PH54B/PHRE1 hybrid is taller and higher eared with higher stalk lodging resistance than the PHJ40/PHT73 hybrid.
TABLE 2A
__________________________________________________________________________
PAIRED INBRED COMPARISON DATA
VARIETY #1 = PH54B
VARIETY #2 = PHR25
__________________________________________________________________________
BU BU TST SDG EST TIL GDU GDU
ACR ACR MST WT VGR CNT LER SHD SLK
VAR#
ABS % MN
ABS ABS ABS ABS ABS ABS ABS
__________________________________________________________________________
TOTAL SUM
1 61.4 101 15.2
59.8
5.9 44.0
0.6 109.4
112.1
2 56.9 94 14.6
58.8
4.6 34.0
2.2 112.9
115.1
LOCS
2 2 2 2 23 39 32 56 56
REPS
4 4 4 4 26 50 42 66 66
DIFF
4.5 7 0.5 1.0 1.3 10.0
1.6 3.5 3.0
PROB
.206 .217
.694
.407
.000#
.000#
.002#
.000#
.000#
__________________________________________________________________________
POL POL POL TAS TAS PLT EAR RT
WT WT SC BLS SZ HT HT LDG
VAR#
ABS % MN
ABS ABS ABS ABS ABS ABS
__________________________________________________________________________
TOTAL SUM
1 73.3 66 5.4 9.0 4.9 76.6 31.6
32.6
2 129.7
115 5.7 8.7 5.8 72.5 28.6
61.4
LOCS
15 15 22 5 16 26 26 4
REPS
20 20 27 7 17 29 29 4
DIFF
56.4 48 0.4 0.3 0.8 4.1 3.0 28.8
PROB
.001#
.001#
.242 .070*
.038+
.000#
.002#
.286
__________________________________________________________________________
STA STK BRT GRN SCT EAR TEX EAR
GRN LDG STK APP GRN SZ EAR MLD
VAR#
ABS ABS ABS ABS ABS ABS ABS ABS
__________________________________________________________________________
TOTAL SUM
1 2.6 97.1
100.0
5.0 6.5 4.5 3.0 5.8
2 3.6 98.6
95.7 4.2 6.3 5.5 2.0 6.8
LOCS
5 3 2 2 4 2 3 5
REPS
7 5 2 4 5 2 3 6
DIFF
1.0 1.5 4.3 0.8 0.3 1.0 1.0 1.0
PROB
.142 .191
.500 .344 .638 .000#
.000#
.266
__________________________________________________________________________
BAR DRP NLF HD
PLT EAR BLT SMT
VAR# ABS ABS ABS ABS
__________________________________________________________________________
TOTAL SUM 1 99.1
97.0 2.0 100.0
2 98.5
99.0 2.0 100.0
LOCS 10 2 1 2
REPS 12 4 1 4
DIFF 0.6 2.1 0.0 0.0
PROB .607
.012+ 1.00
__________________________________________________________________________
* = 10% SIG + = 5% SIG # = 1% SIG
TABLE 2B
__________________________________________________________________________
PAIRED INBRED COMPARISON DATA
VARIETY #1 = PH54B
VARIETY #2 = PHN80
__________________________________________________________________________
BU BU TST SDG EST TIL
ACR ACR MST WT VGR CNT LER
VAR#
ABS % MN
ABS ABS ABS ABS ABS
__________________________________________________________________________
TOTAL SUM
1 61.4
101 15.2
59.8
6.0 33.8
0.0
2 35.6
59 14.2
59.2
4.3 27.9
1.9
LOCS
2 2 2 2 8 7 3
REPS
4 4 4 4 11 10 5
DIFF
25.8
43 1.0 0.6 1.8 5.9 1.9
PROB
.072*
.090*
.672
.590
.016+
.052*
.190
__________________________________________________________________________
GDU GDU POL TAS PLT EAR RT
SHD SLK SC SZ HT HT LDG
VAR#
ABS ABS ABS ABS ABS ABS ABS
__________________________________________________________________________
TOTAL SUM
1 102.6
105.5
4.7 5.3 78.2
33.3
91.7
2 102.4
105.0
4.7 4.3 68.4
27.7
100.0
LOCS
14 14 3 3 8 8 1
REPS
16 16 3 3 10 10 1
DIFF
0.1 0.6 0.0 1.0 9.8 5.6 8.3
PROB
.926
.678
.000#
.225
.001#
.007#
__________________________________________________________________________
STA STK GRN EAR BAR DRP
GRN LDG APP MLD PLT EAR
VAR#
ABS ABS ABS ABS ABS ABS
__________________________________________________________________________
TOTAL SUM
1 2.0 95.6 5.0 5.0 99.3 99.0
2 2.0 98.5 4.8 5.0 95.7 99.3
LOCS
2 2 2 1 2 2
REPS
4 4 4 1 4 3
DIFF
0.0 2.9 0.3 0.0 3.6 0.4
PROB
1.00
.275 .910 .376
__________________________________________________________________________
* = 10% SIG + = 5% SIG # = 1% SIG
TABLE 2C
__________________________________________________________________________
PAIRED INBRED COMPARISON DATA
VARIETY #1 = PH54B
VARIETY #2 = PHK05
__________________________________________________________________________
BU BU TST SDG EST TIL GDU GDU
ACR ACR MST WT VGR CNT LER SHD SLK
VAR#
ABS % MN
ABS ABS ABS ABS ABS ABS ABS
__________________________________________________________________________
TOTAL SUM
1 61.4
101 15.2
59.8
5.9 45.5
0.5 110.8
113.1
2 38.3
63 15.3
60.5
5.9 39.9
6.1 98.9
101.1
LOCS
2 2 2 2 20 44 35 58 58
REPS
4 4 4 4 23 72 50 71 71
DIFF
23.1
38 0.1 0.7 0.0 5.6 5.6 11.9
12.0
PROB
.131
.147
.902
.492
.908
.005#
.007#
.000#
.000#
__________________________________________________________________________
POL POL POL TAS TAS PLT EAR RT
WT WT SC BLS SZ HT HT LDG
VAR#
ABS %MN ABS ABS ABS ABS ABS ABS
__________________________________________________________________________
TOTAL SUM
1 99.5
73 5.5 9.0 5.1 77.7
31.3
32.6
2 155.1
120 6.0 8.2 5.6 68.2
24.8
45.6
LOCS
14 14 27 6 14 24 24 4
REPS
23 23 35 9 15 27 27 4
DIFF
55.6
47 0.5 0.8 0.5 9.5 6.5 13.0
PROB
.001#
.001#
.064*
.363 .089*
.000#
.000#
.569
__________________________________________________________________________
STA STK BRT GRN SCT EAR TEX EAR
GRN LDG STK APP GRN SZ EAR MLD
VAR#
ABS ABS ABS ABS ABS ABS ABS ABS
__________________________________________________________________________
TOTAL SUM
1 2.6 95.6 100.0
5.0 5.0 4.0 3.3 5.8
2 3.2 94.6 100.0
4.8 8.0 3.5 8.0 7.0
LOCS
5 2 2 2 2 2 3 4
REPS
7 4 2 4 3 2 3 6
DIFF
0.6 1.0 0.0 0.3 3.0 0.5 4.7 1.3
PROB
.414
.674 1.00 .874 .374 .500
.020+
.194
__________________________________________________________________________
BAR DRP NLF GOS HD FUS
PLT EAR BLT WLT SMT ERS
VAR#
ABS ABS ABS ABS ABS ABS
__________________________________________________________________________
TOTAL SUM 1 99.4 97.0 2.9 7.5 98.5 4.0
2 98.2 99.8 5.1 5.5 82.5 3.0
LOCS
9 2 3 1 3 1
REPS
11 4 6 2 6 2
DIFF
1.3 2.8 2.2 2.0 16.0 1.0
PROB
.404 .161 .031+ .287
__________________________________________________________________________
GIB EYE HC ECB ECB ECB
ERS SPT BLT DPE 1LF 2SC
VAR#
ABS ABS ABS ABS ABS ABS
__________________________________________________________________________
TOTAL SUM 1 5.3 3.0 6.7 62.5 2.7 5.8
2 5.5 5.5 7.3 75.0 6.2 3.3
LOCS
2 1 1 1 3 3
REPS
4 2 3 2 6 6
DIFF
0.3 2.5 0.7 12.5 3.5 2.5
PROB
.874 .136 .038+
__________________________________________________________________________
* = 10% SIG + = 5% SIG # = 1% SIG
TABLE 2D
__________________________________________________________________________
PAIRED INBRED COMPARISON DATA
VARIETY #1 = PH54B
VARIETY #2 = PHT73
__________________________________________________________________________
SDG EST TIL GDU GDU POL POL
VGR CNT LER SHD SLK WT WT
VAR#
ABS ABS ABS ABS ABS ABS % MN
__________________________________________________________________________
TOTAL SUM
1 5.9 46.7
0.6 110.3
112.7
79.0
63
2 5.4 40.7
8.1 103.8
107.6
79.8
64
LOCS
19 38 34 50 50 12 12
REPS
19 49 47 61 61 21 21
DIFF
0.5 6.0 7.5 6.5 5.1 0.8 1
PROB
.126
.001#
.007#
.000#
.000#
.908
.934
__________________________________________________________________________
POL TAS TAS PLT EAR RT
SC BLS SZ HT HT LDG
VAR#
ABS ABS ABS ABS ABS ABS
__________________________________________________________________________
TOTAL SUM
1 5.5 9.0 4.9 75.3 30.5
32.6
2 5.3 9.0 4.1 60.7 18.6
91.3
LOCS
25 6 16 22 22 4
REPS
33 9 17 23 23 4
DIFF
0.2 0.0 0.9 14.6 12.0
58.7
PROB
.381 1.00 .014+
.000#
.000#
.043+
__________________________________________________________________________
STA BRT SCT EAR TEX EAR
GRN STK GRN SZ EAR MLD
VAR#
ABS ABS ABS ABS ABS ABS
__________________________________________________________________________
TOTAL SUM
1 3.0 100.0
6.3 4.5 3.0 5.8
2 1.3 93.3 7.0 3.5 4.7 7.8
LOCS
3 2 3 2 3 4
REPS
3 2 4 2 3 6
DIFF
1.7 6.7 0.7 1.0 1.7 2.0
PROB
.130 .181 .529 .500 .038+
.236
__________________________________________________________________________
BAR NLF GOS HD FUS GIB
PLT BLT WLT SMT ERS ERS
VAR#
ABS ABS ABS ABS ABS ABS
__________________________________________________________________________
TOTAL SUM
1 99.5 2.9 7.5 98.9 4.0 5.8
2 90.8 1.8 4.0 84.5 5.0 5.8
LOCS
7 3 1 4 1 3
REPS
7 6 2 8 2 6
DIFF
8.7 1.1 3.5 14.4 1.0 0.0
PROB
.231 .362 .045+ .000#
__________________________________________________________________________
EYE HC ECB ECB ECB
SPT BLT DPE 1LF 2SC
VAR #
ABS ABS ABS ABS ABS
__________________________________________________________________________
TOTAL SUM 1 3.0 6.7 62.5 2.7 5.8
2 4.5 7.7 100.0
7.0 2.5
LOCS 1 1 1 3 3
REPS 2 3 2 6 6
DIFF 1.5 1.0 37.5 4.3 3.3
PROB .100 .063*
__________________________________________________________________________
* = 10% SIG + = 5% SIG # = 1% SIG
TABLE 2E
__________________________________________________________________________
PAIRED INBRED COMPARISON DATA
VARIETY #1 = PH54B
VARIETY #2 = PHG68
__________________________________________________________________________
SDG EST TIL GDU GDU POL POL
VGR CNT LER SHD SLK WT WT
VAR#
ABS ABS ABS ABS ABS ABS % MN
__________________________________________________________________________
TOTAL SUM
1 6.1 46.7
0.6 111.4
113.9
92.3
75
2 3.7 40.8
1.2 110.8
110.7
126.5
103
LOCS
16 40 33 52 52 24 24
REPS
16 65 46 63 63 33 33
DIFF
2.4 5.9 0.6 0.5 3.2 34.2
28
PROB
.000#
.000#
.196
.236
.000#
.000#
.000#
__________________________________________________________________________
POL TAS TAS PLT EAR RT STA
SC BLS SZ HT HT LDG GRN
VAR#
ABS ABS ABS ABS ABS ABS ABS
__________________________________________________________________________
TOTAL SUM
1 5.5 9.0 4.9 74.0
29.8
37.3
3.0
2 5.4 9.0 4.5 60.0
21.2
99.3
3.3
LOCS
26 6 17 20 20 3 3
REPS
34 9 18 21 21 3 3
DIFF
0.1 0.0 0.4 14.0
8.6 62.0
0.3
PROB
.716
1.00
.188
.000#
.000#
.147
.423
__________________________________________________________________________
BRT SCT EAR TEX EAR BAR HD
STK GRN SZ EAR MLD PLT SMT
VAR#
ABS ABS ABS ABS ABS ABS ABS
__________________________________________________________________________
TOTAL SUM
1 100.0
6.3 4.5 3.0 5.7 99.4
100.0
2 100.0
6.3 3.5 4.0 8.3 97.6
91.5
LOCS
2 3 2 2 3 6 2
REPS
2 4 2 2 4 6 4
DIFF
0.0 0.0 1.0 1.0 2.7 1.8 8.5
PROB
1.00
.000#
.000#
.000#
.270
.115
.347
__________________________________________________________________________
* = 10% SIG + = 5% SIG # = 1% SIG
TABLE 2F
__________________________________________________________________________
PAIRED INBRED COMPARISON DATA
VARIETY #1 = PH54B
VARIETY #2 = PHM81
__________________________________________________________________________
BU BU TST SDG EST TIL GDU GDU
ACR ACR MST WT VGR CNT LER SHD SLK
VAR#
ABS % MN
ABS ABS ABS ABS ABS ABS ABS
__________________________________________________________________________
TOTAL SUM
1 65.2
100 18.8
53.6
5.9 43.4
0.7 110.0
111.7
2 62.4
96 19.8
56.6
3.6 36.4
2.7 109.1
109.5
LOCS
9 9 15 10 19 35 21 37 35
REPS
17 17 25 20 24 47 25 42 38
DIFF
2.8 4 0.9 3.0 2.2 7.0 2.0 0.9 2.2
PROB
.621
.717
.178
.001#
.000#
.000#
.243
.158
.002#
__________________________________________________________________________
POL TAS TAS PLT EAR RT STA STK BRT
SC BLS SZ HT HT LDG GRN LDS STK
VAR#
ABS ABS ABS ABS ABS ABS ABS ABS ABS
__________________________________________________________________________
TOTAL SUM
1 5.1 9.0 4.8 75.4
30.4
91.7
2.7 97.7
100.0
2 6.3 9.0 5.2 54.6
19.3
100.0
4.6 92.8
100.0
LOCS
15 3 13 25 25 1 5 9 1
REPS
15 3 14 32 32 1 8 18 2
DIFF
1.2 0.0 0.3 20.8
11.1
8.3 1.9 5.0 0.0
PROB
.036+
1.00
.487
.000#
.000# .005#
.173
__________________________________________________________________________
GRN SCT EAR TEX EAR BAR DRP HD
APP GRN SZ EAR MLD PLT EAR SMT
VAR#
ABS ABS ABS ABS ABS ABS ABS ABS
__________________________________________________________________________
TOTAL SUM
1 5.3 6.5 4.5 3.0 5.0 97.1
99.2
100.0
2 6.5 7.3 5.5 5.0 7.5 96.2
100.0
100.0
LOCS
6 4 2 2 4 17 8 2
REPS
12 5 2 2 5 20 16 4
DIFF
1.2 0.8 1.0 2.0 2.5 0.9 0.8 0.0
PROB
.141
.391 .500 .000#
.063*
.664
.193
1.00
__________________________________________________________________________
* = 10% SIG + = 5% SIG # = 1% SIG
TABLE 3A
______________________________________
Average Inbred By Tester Performance Comparing PHR25
To PH54B Crossed To The Same Inbred Testers And Grown In
The Same Experiments. All Values Are Expressed As Percent Of
The Experiment Mean Except Predicted RM, Selection Index,
and Yield (BU/ACR)
SEL BU ECB
INBRED IND ACR YLD 2SC
______________________________________
TOTAL REPLIC. 10 10 10 1
MEAN WTS PHR25 100 118 95 7
MEAN WTS PH54B 104 121 98 5
DIFF 4 3 2 2
______________________________________
TABLE 3B
__________________________________________________________________________
Average Inbred By Tester Performance Comparing PHK05 To PH54B Crossed
To The Same Inbred Testers And Grown In The Same Experiments. All
Values Are Expressed As Percent Of The Experiment Mean Except
Predicted RM, Selection Index, and Yield (BU/ACR)
__________________________________________________________________________
SEL BU PRM TST
INBRED
PRM IND ACR YLD SHD MST WT
__________________________________________________________________________
TOTAL REPLIC.
32 32 32 32 4 33 26
MEAN WTS
PHK05 76 96 115 95 83 93 55
MEAN WTS
PH54B 79 109 129 106 86 96 53
DIFF 2 13 14 12 4 3 2
__________________________________________________________________________
SDG EST GDU GDU STK PLT EAR
INBRED
VGR CNT SHD SLK CNT HT HT
__________________________________________________________________________
TOTAL REPLIC.
23 24 6 2 32 18 18
MEAN WTS
PHK05 109 103 99 98 100 102 105
MEAN WTS
PH54B 97 99 102 102 100 101 108
DIFF 12 4 4 4 0 1 3
__________________________________________________________________________
RT STA STK BRT GRN DRP
INBRED
LDG GRN LDG STK APP EAR
__________________________________________________________________________
TOTAL REPLIC.
10 20 32 9 15 26
MEAN WTS
PHK05 81 93 99 100 100 100
MEAN WTS
PH54B 104 76 104 100 100 99
DIFF 23 17 5 1 1 1
__________________________________________________________________________
TABLE 4A
__________________________________________________________________________
VARIETY #1 = PH54B/PHRE1
VARIETY #2 = PHAJ0/PHRE1
__________________________________________________________________________
VAR PRM BU ACR
BU ACR
MST
# PRM SHD ABS % MN % MN TST WT ABS
SDG VGR %
__________________________________________________________________________
MN
TOTAL SUM
1 80 87 126.7 106 95 53.0 94
2 81 83 117.2 98 95 54.9 105
LOCS
10 11 54 54 55 33 57
REPS
10 11 76 76 77 44 94
DIFF
0 4 9.5 8 0 2.0 10
PROB
.747 .000#
.000# .000#
.978 .000# .003#
__________________________________________________________________________
VAR EST CNT
GDU SHD
GDU SLK
# % MN % MN % MN STK CNT % MN
PLT HT % MN
EAR HT %
__________________________________________________________________________
MN
TOTAL SUM
1 99 102 101 99 102 108
2 101 98 97 100 98 102
LOCS
50 21 14 75 36 36
REPS
85 33 22 108 53 53
DIFF
3 4 4 0 4 6
PROB
.020+ .000# .000# .327 .000# .000#
__________________________________________________________________________
VAR RT LDG
STA GRN
STK LDG
BRT GRN DRP
# % MN % MN % MN STK % MN APP % MN EAR % MN
__________________________________________________________________________
TOTAL SUM
1 93 85 104 100 98 100
2 98 112 100 100 102 100
LOCS
28 28 47 10 19 33
REPS
42 38 66 10 23 40
DIFF
5 27 4 1 4 1
PROB
.115 .000# .001# .193 .308 .022+
__________________________________________________________________________
NLF GOS HD FUS GIB EYE ECB ECB ECB
VAR BLT WLT SMT ERS ERS SPT 1LF 2SC 2IT
# ABS ABS ABS ABS ABS ABS ABS ABS ABS
__________________________________________________________________________
TOTAL SUM
1 5.3 5.5 99.2 3.5 6.5 4.5 1.8 5.3 5.9
2 6.3 5.0 100.0
3.5 6.9 5.0 2.5 5.2 4.3
LOCS
2 1 3 1 4 1 3 3 1
REPS
5 2 6 2 8 2 8 7 3
DIFF
1.1 0.5 0.8 0.0 0.4 0.5 0.7 0.1 1.7
PROB
.314 .423 .391 .184 .926
__________________________________________________________________________
*= 10% SIG + = 5% SIG # = 1% SIG
TABLE 4B
__________________________________________________________________________
VARIETY #1 = PH54B/PHRE1
VARIETY #2 = PH90A/PHRE1
__________________________________________________________________________
VAR BU TST SDG
# PRM PRM SHD
BU ACR ABS
ACR % MN
MST % MN
WT ABS
VGR %
__________________________________________________________________________
MN
TOTAL SUM
1 80 87 123.8 106 95 53.0 95
2 83 87 116.2 100 100 54.1 95
LOCS
9 11 52 52 53 33 52
REPS
9 11 67 67 68 42 84
DIFF
3 0 7.5 6 4 1.2 0
PROB
.053*
.597 .001# .007# .002# .004#
.915
__________________________________________________________________________
VAR EST CNT
GDU SHD
GDU SLK
# % MN % MN % MN STK CNT % MN
PLT HT % MN
EAR HT %
__________________________________________________________________________
MN
TOTAL SUM
1 98 102 100 99 101 107
2 98 102 102 100 94 94
LOCS
45 17 10 63 30 30
REPS
70 26 16 85 39 39
DIFF
0 0 2 1 7 13
PROB
.943 .376 .000# .205 .000# .000#
__________________________________________________________________________
VAR RT LDG
STA GRN
STK LDG GRN DRP
# % MN % MN % MN BRT STK % MN
APP % MN EAR % MN
__________________________________________________________________________
TOTAL SUM
1 98 83 104 100 99 100
2 107 89 100 100 100 100
LOCS
20 25 44 10 19 32
REPS
28 34 56 10 21 34
DIFF
9 7 4 0 1 0
PROB
.002# .451 .021+ .443 .781 .051*
__________________________________________________________________________
NLF GOS HD FUS GIB EYE ECB ECB ECB
VAR BLT WLT SMT ERS ERS SPT 1LF 2SC 2IT
# ABS ABS ABS ABS ABS ABS ABS ABS ABS
__________________________________________________________________________
TOTAL SUM
1 5.3 5.5 99.2 3.5 6.1 4.5 2.6 4.0 6.8
2 5.5 5.5 100.0
2.5 6.5 5.0 2.7 4.9 5.9
LOCS
2 1 3 1 4 1 3 3 1
REPS
5 2 6 2 7 2 8 7 3
DIFF
0.3 0.0 0.8 1.0 0.4 0.5 0.1 0.9 0.9
PROB
.500 .423 .591 .840 .319
__________________________________________________________________________
*= 10% SIG + = 5% SIG # = 1% SIG
TABLE 4C
__________________________________________________________________________
VARIETY #1 = PH54B/PHRE1
VARIETY #2 = PHKM5/PHRE1
__________________________________________________________________________
VAR PRM BU ACR
BU ACR MST TST WT SDG VGR
# PRM SHD ABS % MN % MN ABS % MN
__________________________________________________________________________
TOTAL SUM
1 81 86 126.4 106 96 52.3 94
2 82 85 127.7 107 98 54.5 108
LOCS
7 8 36 36 36 19 40
REPS
7 8 54 54 54 30 71
DIFF
2 1 1.3 1 2 2.3 13
PROB
.417 .023+ .657 .742 .267 .000# .004#
__________________________________________________________________________
VAR EST CNT
GDU SHD
GDU SLK
# % MN % MN % MN STK CNT % MN
PLT HT % MN
EAR HT %
__________________________________________________________________________
MN
TOTAL SUM
1 99 103 101 99 103 108
2 99 101 100 100 98 101
LOCS
36 18 10 52 23 23
REPS
69 30 16 83 38 38
DIFF
0 2 1 1 5 7
PROB
.947 .001# .007# .087* .000# .000#
__________________________________________________________________________
VAR RT LDG
STA GRN
STK LDG GRN DRP
# % MN % MN % MN BRT STK % MN
APP % MN EAR % MN
__________________________________________________________________________
TOTAL SUM
1 92 93 105 100 95 100
2 107 104 107 98 95 100
LOCS
20 17 29 4 9 15
REPS
33 25 47 4 13 22
DIFF
15 12 3 2 0 0
PROB
.007# .288 .079* .525 .930 .216
__________________________________________________________________________
NLF GOS HD FUS GIB EYE ECB ECB ECB
VAR BLT WLT SMT ERS ERS SPT 1LF 2SC 2IT
# ABS ABS ABS ABS ABS ABS ABS ABS ABS
__________________________________________________________________________
TOTAL SUM
1 5.3 5.5 98.8 3.5 6.5 4.5 2.6 3.5 6.8
2 6.3 4.5 92.4 3.5 6.0 4.5 3.9 3.7 4.5
LOCS
2 1 2 1 4 1 3 2 1
REPS
5 2 4 2 8 2 8 6 3
DIFF
1.1 1.0 6.4 0.0 0.5 0.0 1.3 0.2 2.3
PROB
.234 .189 .182 .122 .500
__________________________________________________________________________
*= 10% SIG + = 5% SIG # = 1% SIG
TABLE 4D
__________________________________________________________________________
VARIETY #1 = PH54B/PHFA5
VARIETY #2 = PHT73/PHFA5
__________________________________________________________________________
BU BU TST SDG EST GDU
VAR PRM ACR ACR MST WT VGR CNT SHD
# PRM SHD ABS % MN % MN ABS % MN % MN %
__________________________________________________________________________
MN
TOTAL SUM
1 81 79 127.3
114 98 52.8 103 116 96
2 79 77 96.1 86 93 52.9 79 84 95
LOCS
2 2 6 6 6 3 7 8 4
REPS
2 2 16 16 16 8 14 18 8
DIFF
2 2 31.2 28 5 0.2 23 32 1
PROB
.144 .298 .000#
.000# .020+
.671 .105 .012+ .622
__________________________________________________________________________
VAR GDU SLK
STK CNT
PLT HT
EAR HT RT LDG
STA GRN
STK LDG
GRN APP
# % MN % MN % MN % MN % MN % MN % MN %
__________________________________________________________________________
MN
TOTAL SUM
1 94 102 101 102 98 128 115 112
2 102 89 94 77 98 37 88 125
LOCS
1 8 4 4 4 4 6 1
REPS
1 18 12 12 10 10 16 4
DIFF
8 13 7 28 0 90 27 13
PROB .049+ .033+ .013+ .801 .007# .024+
__________________________________________________________________________
*= 10% SIG + = 5% SIG # = 1% SIG
TABLE 4E
__________________________________________________________________________
VARIETY #1 = PH54B/PHFA5
VARIETY #2 = PHG68/PHFA5
__________________________________________________________________________
BU BU TST SDG EST
VAR PRM ACR ACR MST WT VGR CNT
# PRM SHD ABS % MN % MN ABS % MN % MN
__________________________________________________________________________
TOTAL SUM
1 82 79 144.8
111 99 53.0 86 112
2 81 81 126.1
94 97 55.0 83 100
LOCS
3 2 11 11 11 2 11 12
REPS
3 2 21 21 21 4 18 22
DIFF
1 2 18.7 17 2 2.0 3 12
PROB
.236 .130 .003#
.006#
.169 .056*
.510 .006#
__________________________________________________________________________
GDU STK PLT EAR RT STA STK GRN
VAR SHD CNT HT HT LDG GRN LDG APP
# % MN % MN % MN % MN % MN % MN % MN % MN
__________________________________________________________________________
TOTAL SUM
1 98 101 102 103 99 117 108 112
2 99 101 99 88 101 72 97 116
LOCS
6 13 3 3 8 6 11 1
REPS
11 23 8 8 14 10 21 4
DIFF
1 0 3 16 2 46 12 4
PROB
.245 .482 .469 .160 .247 .012+
.017+
__________________________________________________________________________
*= 10% SIG + = 5% SIG # = 1% SIG
TABLE 4F
__________________________________________________________________________
VARIETY #1 = PHRE1/PH54B
VARIETY #2 = PHRE1/PHK05
__________________________________________________________________________
VAR PRM BU ACR
BU ACR MST TST SDG VGR
# PRM SHD ABS % MN % MN WT ABS % MN
__________________________________________________________________________
TOTAL SUM
1 80 86 121.4 108 95 51.8 94
2 78 82 110.0 97 92 53.9 99
LOCS
12 13 64 64 65 38 62
REPS
12 13 94 94 95 54 91
DIFF
2 4 11.4 11 3 2.1 5
PROB
.087* .000# .000# .000# .003# .000# .129
__________________________________________________________________________
VAR EST CNT
GDU SHD
GDU SLK
# % MN % MN % MN STK CNT % MN
PLT HT % MN
EAR HT %
__________________________________________________________________________
MN
TOTAL SUM
1 99 103 102 99 103 109
2 103 98 97 100 102 107
LOCS
53 25 14 91 42 41
REPS
84 43 23 139 66 64
DIFF
4 5 5 1 0 2
PROB
.001# .000# .000# .136 .451 .081*
__________________________________________________________________________
VAR RT LDG
STA GRN
STK LDG
BRT STK GRN DRP
# % MN % MN % MN % MN APP % MN EAR % MN
__________________________________________________________________________
TOTAL SUM
1 93 84 105 100 97 99
2 87 88 96 100 104 100
LOCS
28 30 55 10 27 38
REPS
40 37 83 10 42 51
DIFF
6 4 9 1 7 0
PROB
.221 .620 .001# .193 .056* .485
__________________________________________________________________________
NLF GOS HD FUS GIB EYE ECB ECB ECB
VAR BLT WLT SMT ERS ERS SPT 1LF 2SC 2IT
# ABS ABS ABS ABS ABS ABS ABS ABS ABS
__________________________________________________________________________
TOTAL SUM
1 5.3 5.5 98.8 3.5 6.5 4.5 1.8 5.5 5.9
2 5.7 6.0 96.1 4.0 6.6 6.0 4.8 4.7 4.0
LOCS
2 1 2 1 4 1 3 2 1
REPS
5 2 4 2 8 2 8 6 3
DIFF
0.4 0.5 2.6 0.5 0.1 1.5 3.0 0.8 1.9
PROB
.126 .500 .789 .035+
.500
__________________________________________________________________________
*= 10% SIG + = 5% SIG # = 1% SIG
TABLE 5A
__________________________________________________________________________
VARIETY #1 = PHRE1/PH54B
VARIETY #2 = PHK05/PHH51
__________________________________________________________________________
VAR BU ACR BU ACR
MST TST WT SDG VGR
# PRM PRM SHD
ABS % MN % MN ABS % MN
__________________________________________________________________________
TOTAL SUM
1 79 86 116.6 111 95 51.3 102
2 76 75 98.6 94 88 53.3 102
LOCS
9 11 52 52 52 36 44
REPS
9 11 81 81 81 55 67
DIFF
3 10 18.0 17 7 2.0 1
PROB
.007# .000# .000# .000# .000# .000# .877
__________________________________________________________________________
VAR EST CNT
GDU SHD
GDU SLK
# % MN % MN % MN STK CNT % MN
PLT HT % MN
EAR HT %
__________________________________________________________________________
MN
TOTAL SUM
1 99 103 101 100 102 108
2 103 91 92 100 98 99
LOCS
46 18 8 70 32 31
REPS
74 32 13 111 52 51
DIFF
4 11 9 1 4 10
PROB
.001# .000# .000# .246 .000# .000#
__________________________________________________________________________
VAR RT LDG
STA GRN
STK LDG
BRT GRN DRP
# % MN % MN % MN STK % MN APP % MN EAR % MN
__________________________________________________________________________
TOTAL SUM
1 98 88 105 100 97 99
2 86 87 94 100 114 100
LOCS
19 23 51 9 27 37
REPS
27 31 79 9 42 50
DIFF
12 0 11 1 17 1
PROB
.028+ .970 .000# .195 .000# .015+
__________________________________________________________________________
NLF GOS HD FUS GIB EYE ECB ECB ECB
VAR BLT WLT SMT ERS ERS SPT 1LF 2SC 2IT
# ABS ABS ABS ABS ABS ABS ABS ABS ABS
__________________________________________________________________________
TOTAL SUM
1 5.3 5.5 98.8 3.5 6.5 4.5 1.8 5.5 5.9
2 2.6 3.5 82.9 4.5 7.0 5.5 4.9 4.0 6.7
LOCS
2 1 2 1 4 1 3 2 1
REPS
5 2 4 2 7 2 8 6 3
DIFF
2.7 2.0 15.8 1.0 0.5 1.0 3.1 1.5 0.8
PROB
.079* .382 .495 .009#
.070*
__________________________________________________________________________
*= 10% SIG + = 5% SIG # = 1% SIG
TABLE 5B
__________________________________________________________________________
VARIETY #1 = PHRE1/PH54B
VARIETY #2 = PHJ40/PHT73
__________________________________________________________________________
VAR PRM BU ACR BU ACR
MST TST WT SDG VGR
# PRM SHD ABS % MN % MN ABS % MN
__________________________________________________________________________
TOTAL SUM
1 79 86 116.0 110 93 51.5 103
2 82 82 108.4 102 101 51.8 107
LOCS
8 10 47 47 48 32 49
REPS
8 10 74 74 75 51 92
DIFF
3 3 7.6 8 7 0.3 4
PROB
.002# .000# .000# .000# .000# .232 .172
__________________________________________________________________________
VAR EST CNT
GDU SHD
GDU SLK
# % MN % MN % MN STK CNT % MN
PLT HT % MN
EAR HT %
__________________________________________________________________________
MN
TOTAL SUM
1 98 102 102 100 101 109
2 100 98 99 100 98 98
LOCS
51 12 6 61 30 29
REPS
93 21 12 101 50 48
DIFF
2 3 3 1 3 10
PROB
.034+ .000# .012+ .147 .000# .000#
__________________________________________________________________________
VAR RT LDG
STA GRN
STK LDG
BRT GRN DRP
# % MN % MN % MN STK % MN APP % MN EAR % MN
__________________________________________________________________________
TOTAL SUM
1 96 81 105 100 97 99
2 99 72 102 100 92 100
LOCS
16 21 44 7 21 29
REPS
25 31 68 7 33 40
DIFF
3 9 3 0 5 1
PROB
.521 .159 .008# .690 .278 .039+
__________________________________________________________________________
NLF GOS HD FUS GIB EYE ECB ECB ECB
VAR BLT WLT SMT ERS ERS SPT 1LF 2SC 2IT
# ABS ABS ABS ABS ABS ABS ABS ABS ABS
__________________________________________________________________________
TOTAL SUM
1 5.3 5.5 98.8 3.5 6.5 4.5 1.8 5.5 5.9
2 5.3 4.0 100.0
3.5 6.8 4.0 4.1 5.0 4.1
LOCS
2 1 2 1 4 1 3 2 1
REPS
5 2 4 2 8 2 8 6 3
DIFF
0.0 1.5 1.3 0.0 0.3 0.5 2.3 0.5 1.8
PROB
.000# .500 .391 .071*
.500
__________________________________________________________________________
*= 10% SIG + = 5% SIG # = 1% SIG
Deposits
Applicant has made a deposit of at least 2500 seeds of Inbred Corn Line PH54B with the American Type Culture Collection (ATCC), Rockville, Md. 20852 USA, ATCC Deposit No. 97496. The seeds deposited with the ATCC on Mar. 29, 1996 were taken from the deposit maintained by Pioneer Hi-Bred International. Inc.. 700 Capital Square. 400 Locust Street, Des Moines, Iowa 50309-2340 since prior to the filing date of this application. This deposit of the Inbred Corn Line PH54B will be maintained in the ATCC depository, which is a public depository, for a period of 30 years, or 5 years after the most recent request, or for the effective life of the patent, whichever is longer, and will be replaced if it becomes nonviable during that period. Additionally. Applicant has satisfied all the requirements of 37 C.F.R. .sctn..sctn.1.801-1.809, including providing an indication of the viability of the sample. Applicant imposes no restrictions on the availability of the deposited material from the ATCC; however, Applicant has no authority to waive any restrictions imposed by law on the transfer of biological material or its transportation in commerce. Applicant does not waive any infringement of its rights granted under this patent or under the Plant Variety Protection Act (7 USC 2321 et seq.). Applicant has applied for U.S. Plant Variety Protection of PH54B under Application No, 9500223.
The foregoing invention has been described in detail by way of illustration and example for purposes of clarity and understanding. However, it will be obvious that certain changes and modifications such as single gene modifications and mutations, somoclonal variants, variant individuals selected from large populations of the plants of the instant inbred and the like may be practiced within the scope of the invention, as limited only by the scope of the appended claims.
Claims
1. Seed of maize inbred line designated PH54B and having ATCC Accession No. 97496.
2. A maize plant produced by the seed of claim 1 and its plant parts.
3. Pollen of the plant of claim 2.
4. An ovule of the plant of claim 2.
5. A maize plant developed from the seed of maize inbred line designated PH54B, the seed of which have been deposited under ATCC Accession No. 97496, said maize plant having all the physiological and morphological characteristics of maize inbred line designated PH54B, the seed of such having been deposited under ATCC Accession No. 97496.
6. A male sterile maize plant otherwise having all the physiological and morphological characteristics of maize inbred line designated PH54B, the seed of which have been deposited under ATCC Accession No. 97496.
7. A tissue culture of regenerable cells of an inbred maize plant PH54B, wherein the tissue culture regenerates plants capable of expressing all the morphological and physiological characteristics of the maize plant named PH54B, the seed of which have been deposited under ATCC Accession No. 97496.
8. The tissue culture of claim 7, the tissue culture selected from the group consisting of leaves, pollen, embryos, roots, root tips, anthers, silk, flowers, kernels, ears, cobs, husks, stalks, cells and protoplasts thereof.
9. A maize plant regenerated from the tissue culture of claim 7, capable of expressing all the morphological and physiological characteristics of inbred PH54B the seed of which have been deposited under ATCC Accession No. 97496.
10. A method for producing first generation (F.sub.1) hybrid maize seed comprising crossing a first inbred parent maize plant with a second inbred parent maize plant and harvesting the resultant first generation (F.sub.1) hybrid maize seed, wherein said first or second parent maize plant is the maize plant of claim 2.
11. The method of claim 10 wherein inbred maize plant PH54B, the seed of which have been deposited under ATCC Accession No. 97496, is the female parent.
12. The method of claim 10 wherein inbred maize plant PH54B, the seed of which have been deposited under ATCC Accession No, 97496, is the male parent.
13. An F.sub.1 hybrid seed or plant, or parts thereof, produced by crossing an inbred maize plant according to claim 2 with another, different maize plant.
| 160390 | November 1985 | EPX |
- Conger, B. V., et al. (1987) "Somatic Embryogenesis From Cultured Leaf Segments of Zea Mays", Plant Cell Reports, 6:345-347. Duncan, D. R., et al. (1985) "The Production of Callus Capable of Plant Regeneration From Immature Embryos of Numerous Zea Mays Genotypes", Planta, 165:322-332. Edallo, et al. (1981) "Chromosomal Variation and Frequency of Spontaneous Mutation Associated with in vitro Culture and Plant Regeneration in Maize", Maydica, XXVI 39-56. Green, et al., (1975) "Plant Regeneration From Tissue Cultures of Maize", Crop Science, vol. 15, pp. 417-421. Green, C. E., et al. (1982) "Plant Regeneration in Tissue Cultures of Maize" Maize for Biological Research, pp. 367-372. Hallauer, A. R. et al. (1988) "Corn Breeding" Corn and Corn Improvement, No. 18, pp. 463-564. Meghji, M. R., et al. (1984). "Inbreeding Depression, Inbred & Hybrid Grain Yields, and Other Traits of Maize Genotypes Representing Three Eras", Crop Science, vol. 24, pp. 545-549. Phillips, et al. (1988) "Cell/Tissue Culture and In Vitro Manipulation", Corn & Corn Improvement, 3rd Ed., ASA Publication, No. 18, pp. 345-387. Poehlman (1987) Breeding Field Crop, AVI Publication Co., Westport, Ct., pp. 237-246. Rao, K. V., et al., (1986) "Somatic Embryogenesis in Glume Callus Cultures", Maize Genetics Cooperative Newsletter, No. 60, pp. 64-65. Sass, John F. (1977) "Morphology", Corn & Corn Improvement, ASA Publication. Madison, Wisconsin, pp. 89-109. Songstad, D. D. et al. (1988) "Effect of ACC (1-aminocyclopropane-1-carboxyclic acid), Silver Nitrate & Norbonadiene on Plant Regeneration From Maize Callus Cultures", Plant Cell Report, 7:262-265. Tomes, et al. (1985) "The Effect of Parental Genotype on Initiation of Embryogenic Callus From Elite Maize (Zea Mays L.) Germplasm", Theor. Appl. Genet., vol. 70, pp. 505-509. Troyer, et al. (198) "Selection for Early Flowering in Corn: 10 Late Synthetics", Crop Science, vol. 25, pp. 695-697. Umbeck, et al. (1983) "Reversion of Male-Sterile T-Cytoplasm Maize to Male Fertility in Tissue Culture", Crop Science, vol. 23, pp. 584-588. Wright, Harold (1980) "Commercial Hybrid Seed Production", Hybridization of Crop Plants, Ch. 8: 161-176. Wych, Robert D. (1988) "Production of Hybrid Seed", Corn and Corn Improvement, Ch. 9, pp. 565-607. Lee, Michael (1994) "Inbred Lines of Maize and Their Molecular Markers", The Maize Handbook Ch. 65:423-432. Boppenmaier, et al., "Comparsons Among Strains of Inbreds for RFLPs", Maize Genetics Cooperative Newsletter, 65:1991, p. 90. Coe et al. 1988. In Corn and Corn Improvement. Third Edition. Sprague et al., eds. Ch. 3: 81-258.
Type: Grant
Filed: Jan 31, 1995
Date of Patent: Oct 8, 1996
Assignee: Pioneer Hi-Bred International, Inc. (Des Moines, IA)
Inventors: Lori L. Carrigan (Spicer, MN), Vladimir Puskaric (Woodstock)
Primary Examiner: Erich E. Veitenheimer
Law Firm: Pioneer Hi-Bred International, Inc.
Application Number: 8/381,176
International Classification: A01H 102; A01H 400; A01H 500; C12N 504;
