Neuron-specific ICAM-4 promoter

- ICOS Corporation

DNA sequences, derived from the 5' region of the human neuron-specific cellular adhesion molecule ICAM-4, which promote neuron-specific gene transcription are disclosed along with polynucleotides comprising the promoter DNA operatively linked to heterologous gene-encoding polynucleotides, expression vectors comprising the promoter DNA, and host cells transformed or transfected with DNA comprising the promoter sequences.

Skip to:  ·  Claims  ·  References Cited  · Patent History  ·  Patent History

Claims

1. A purified and isolated DNA comprising a promoter of a mammalian ICAM-4 gene, wherein said promoter is characterized by the ability to specifically promote transcription in a neuronal cell of a polynucleotide operatively linked to said promoter, and said promoter comprises a nucleotide sequence which is 5' to the coding sequence of a mammalian ICAM-4 gene, wherein ICAM-4 is characterized as a neuron-specific cellular adhesion molecule.

2. The DNA according to claim 1 wherein said promoter is from the human ICAM-4 gene.

3. The DNA of claim 2 wherein the promoter is comprised within SEQ ID NO: 33.

4. The DNA according to claim 3 wherein said promoter is comprised within a sequence from about nucleotide 1204 to about nucleotide 1666 in SEQ ID NO: 33.

5. The DNA according to any one of claims 1-4 wherein said neuronal cell is a hippocampal cell.

6. A purified and isolated polynucleotide comprising 1) a promoter of a human ICAM-4 gene, wherein ICAM-4 is characterized as a neuron-specific cellular adhesion molecule, wherein said promoter is characterized by the ability to specifically promote transcription in a neuronal cell of a polynucleotide operatively linked to said promoter, and said promoter comprises a nucleotide sequence which is 5' to the coding sequence of human ICAM-4; and 2) a second nucleotide sequence encoding a heterologous gene which is operatively linked to said promoter.

7. The polynucleotide according to claim 6 wherein said promoter is comprised within a sequence from about nucleotide 1204 to about nucleotide 1666 in SEQ ID NO: 33.

8. An expression vector comprising the DNA according to claim 1 or 6.

9. A host cell transformed with the vector according to claim 8.

Referenced Cited
Foreign Patent Documents
468257 A January 1992 EPX
WO 89/02922 April 1989 WOX
WO 90/13300 November 1990 WOX
WO 91/10683 July 1991 WOX
WO 91/16928 November 1991 WOX
WO 92/04034 March 1992 WOX
WO 92/06199 April 1992 WOX
WO 93/14776 August 1993 WOX
Other references
  • Bailly et al. Eur. J. Immunol. 25, 3316-3320, 1995. Sambrook et al. Molecular Cloning : A Laboratory Manual, Second Ed. New York :Cold Spring Harbor Press, p. 2.43, 1989. Stratagene Cloning Systems Catalog, p. 296, 1994. Ashkenazi et al., "Protection against endotoxic shock by tumor necrosis factor receptor immunoadhesin," Proc.Natl.Acad.Sci. (USA) 88:10535-10539 (1991). Capecchi, "Altering the genome by homologous recombination," Science 244:1288-1292 (1989). Capon et al., "Designing CD4 immunoadhesins for AIDS therapy," Nature 337:525-531 (1989). Chomesynski and Sacchi, "Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction," Anal.Biochem. 162:156-159 (1987). de Fourgerolles et al., "Intercellular adhesion molecule 3, a third adhesion counter-receptor for lymphocyte function associated molecule 1 on resting lymphocytes," J.Exp.Med. 175:185-190 (1992). Dustin et al., "Induction by IL-1 and interferon-.gamma.:tissue distribution, biochemistry, and function of a natural adherence molecule (ICAM-1)," J.Immunol. 137:245-254 (1986). Edwards, "Cell adhesion molecules as a target for therapy," Curr.Opin.Ther.Pat. 1:1617-1630 (1991). Frohman, PCR Protocols, "RACE: rapid amplification of cDNA ends," Innis (ed), Acedemic Press: New York, pp. 28-38 (1990). Imamura et al., "Variations by layers and developmental changes in expression of telencephalin in the visual cortex of cats," Neurosci.Letts. 119:118-121 (1990). Kita et al., "Sequence and expression of ratICAM-1," Biochem.Biophys.Acta. 1131;108-110 (1992). Lothman et al., "Kindling with Rapidly Recurring Hippocampal Seizures," Brain.Res. 360:83-91 (1985). Mori et al., "Telencephalon-specific antigen identified by monoclonal antibody," Proc.Natl.Acad.Sci. (USA) 84:3921-3925 (1987). Newman et al., "PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily," Science 247:1219-1222 (1990). Oka et al., "Mammalian telencephalic neurons express a segment-specific membrane glycoprotein, telencephalin," Neuroscience 35:93-103 (1990). Sonderegger et al., "Regulation of axonal growth in the vertebrate nervous system by interactions between glycoproteins belonging to two subgroups of the immunoglobulin superfamily," J. Cell Biol. 119:1387-1394 (1992). Springer, "Adhesion receptors of the immune system, " Nature 346:425-434 (1990). Staunton et al., "Functional cloning of ICAM-2, a cell adhesion ligand for LFA-1 homologous to ICAM-1," Nature 339:61-64 (1989). Vonderheide et al., "Residues within a conserved amino acid motif of domains 1 and 4 of VCAM-1 are required fir binding to VLA-4," J.Cell.Biol 125:215-222 (1994). Xu et al., "Isolation, characterization, and expression of mouse ICAM-2 complementary and genomic DNA," J.Immunol. 149:2650-2655 (1992). Yoshihara et al., "Immunoglobulin superfamily molecules in the nervous system," Neurosci.Res. 10:83-105 (1991). Yoshihiro et al., "An ICAM-related neuronal glycoprotein, telecephalin, with brain segment-specific expression," Neuron 12:541-554 (1994). Yoshihiro et al., "Primary structure of telencephalon-specific neuronal adhesion molecule telencephalin," Neuroscience Supp 18, p. S83 (1994). Yoshihiro et al., "Telencephanlin, a brain segment-specific dendritic glycoprotein, is a novel member of immunoglobulin superfamily," Soc.Neurosci.Abstr. 19 p. 646 (1993).
Patent History
Patent number: 5753502
Type: Grant
Filed: Jun 6, 1996
Date of Patent: May 19, 1998
Assignee: ICOS Corporation (Bothell, WA)
Inventors: Patrick D. Kilgannon (Bothell, WA), W. Michael Gallatin (Mercer Island, WA)
Primary Examiner: John Ulm
Assistant Examiner: Karen E. Brown
Law Firm: Marshall, O'Toole, Gerstein, Murray & Borun
Application Number: 8/656,984