Animal Cell, Per Se (e.g., Cell Lines, Etc.); Composition Thereof; Process Of Propagating, Maintaining Or Preserving An Animal Cell Or Composition Thereof; Process Of Isolating Or Separating An Animal Cell Or Composition Thereof; Process Of Preparing A Composition Containing An Animal Cell; Culture Media Therefore Patents (Class 435/325)
  • Patent number: 12290562
    Abstract: The invention provides a composition useful to prepare influenza vaccine viruses, e.g., in the absence of helper virus, which includes internal viral segments from an influenza virus vaccine strain or isolate, e.g., one that is safe in humans, for instance, one that does not result in significant disease, and encodes a heterologous antigen.
    Type: Grant
    Filed: March 25, 2021
    Date of Patent: May 6, 2025
    Assignee: Wisconsin Alumni Research Foundation (WARF)
    Inventors: Yoshihiro Kawaoka, Gabriele Neumann
  • Patent number: 12286480
    Abstract: Provided herein are, inter alia, methods of identifying agents that are capable of inhibiting the binding (e.g., coupling) between a ROR1 protein and a ROR2 protein. By interfering with ROR1-ROR2 coupling (binding) the agents identified using the methods provided herein inhibit non-canonical Wnt5a signaling. Thus, the agents identified by the methods provided herein may, inter alia, be useful for cancer diagnosis and therapy.
    Type: Grant
    Filed: December 21, 2020
    Date of Patent: April 29, 2025
    Assignee: The Regents of the University of California
    Inventors: Thomas J. Kipps, Jian Yu
  • Patent number: 12286462
    Abstract: The present invention relates to variants of porcine granulocyte colony stimulating factor (pG-CSF). The pG-CSF variants are useful in treating preventing or reducing the incidence of bacterial infections in swine. Methods of treating swine are disclosed.
    Type: Grant
    Filed: January 30, 2023
    Date of Patent: April 29, 2025
    Assignees: Elanco US Inc., Ambrx, Inc.
    Inventors: Peter Connor Canning, Nickolas Knudsen, Md Harunur Rashid
  • Patent number: 12281305
    Abstract: Provided are compounds, methods, and pharmaceutical compositions for reducing the amount or activity of PRNP RNA in a cell or animal, and in certain instances reducing the amount of PrP protein in a cell or animal. Such compounds, methods, and pharmaceutical compositions are useful to ameliorate at least one symptom or hallmark of a neurodegenerative disease. Such symptoms and hallmarks spongiform changes in the brain, development of abnormal protein aggregates, neuronal loss, markers of neuronal loss, rapidly progressing dementia, and death. Such neurodegenerative diseases include prion diseases, Creutzfeldt-Jakob disease (CJD), variant Creutzfeldt-Jakob Disease (vCJD), familial Creutzfeldt-Jakob Disease (fCJD), Gerstmann-Straussler-Scheinker syndrome, fatal familial insomnia, kuru, Alzheimer's disease, or Parkinson's disease.
    Type: Grant
    Filed: November 21, 2019
    Date of Patent: April 22, 2025
    Assignee: Ionis Pharmaceuticals, Inc.
    Inventors: Susan M. Freier, Huynh-Hoa Bui, Hien Thuy Zhao
  • Patent number: 12281152
    Abstract: Chimeric antigen receptors targeted to TAG72 and the use thereof to treat ovarian cancer and other cancers are described.
    Type: Grant
    Filed: August 1, 2019
    Date of Patent: April 22, 2025
    Assignee: City of Hope
    Inventors: Saul J. Priceman, John P. Murad, Stephen J. Forman, John (Jack) E. Shively, Paul Yazaki, David Colcher, Anna Kozlowska, Hee Jun Lee
  • Patent number: 12275783
    Abstract: Described herein are interleukin-10 receptor-2 peptides, antibodies that bind the peptides, compositions including the peptides and antibodies and methods of use of the peptides and antibodies. The interleukin-10 receptor-2 peptide consists of an 8-15 amino acid sequence that includes SEQ?ID?NO:?1 ((I/V)P(P/K/V/E)P(E/K/R/Q)N(A/V)R),? SEQ?ID?NO:?2 ((S/L/V)PAF(A/P)(K/Q)(G/T/E)(N/T/D)),?or? SEQ?ID?NO:?3 (PP(G/T/Q/V)(V/T/A)(R/H/T/S)(GN/NHP/SAA)).
    Type: Grant
    Filed: April 2, 2021
    Date of Patent: April 15, 2025
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Maria K. Dashek, Mark E. Cook
  • Patent number: 12276598
    Abstract: A specimen processing system includes a plate for supporting a specimen system, wherein the specimen system includes a container and a specimen contained therein. The specimen processing system further includes a camera disposed above the plate and configured to generate images of the specimen system, a light source disposed beneath the plate for radiating light towards the plate, a light stop for blocking a portion of the light from reaching the specimen system to produce darkfield illumination of the specimen at the camera, and one or more processors electronically coupled to the camera and configured to track a position of the specimen within the specimen container during a specimen processing protocol based on the images.
    Type: Grant
    Filed: May 16, 2024
    Date of Patent: April 15, 2025
    Assignee: CooperSurgical, Inc.
    Inventors: Joseph Gordon, John Glaberson, Tara Pratap Ebsworth, Patrick N. Gutelius
  • Patent number: 12275960
    Abstract: Provided herein are methods that include introducing into an inner ear of a mammal a therapeutically effective amount of an adeno-associated virus (AAV) vector that includes a nucleotide sequence encoding (a) a polypeptide including an antibody heavy chain variable domain operably linked to a signal peptide and a polypeptide including an antibody light chain variable domain operably linked to a signal peptide; (b) a polypeptide including an antigen-binding antibody fragment operably linked to a signal peptide; or (c) a soluble vascular endothelial growth factor receptor operably linked to a signal peptide.
    Type: Grant
    Filed: June 13, 2023
    Date of Patent: April 15, 2025
    Assignee: Akouos, Inc.
    Inventors: Emmanuel John Simons, Robert Ng, Michael McKenna
  • Patent number: 12272068
    Abstract: A device may receive images of a patient, and may perform segmentation of surfaces on the images to create a 3D model. The device may identify normal tissue regions and atrial fibrosis (AF) regions in the 3D model, and may divide the 3D model into the normal tissue regions and the AF regions. The device may assign first cell and tissue properties to the normal tissue regions, and may assign second cell and tissue properties to the AF regions. The device may perform simulations on the normal tissue regions and the AD regions, based on the first and second cell and tissue properties, to generate simulation results, and may extract first features from the simulation results. The device may extract second features from the images, and may process the first and second features, with a model, to select a feature that is predictive of atrial fibrillation recurrence.
    Type: Grant
    Filed: February 6, 2024
    Date of Patent: April 8, 2025
    Assignee: THE JOHNS HOPKINS UNIVERSITY
    Inventors: Natalia A. Trayanova, Rheeda Ali, Julie Shade
  • Patent number: 12270050
    Abstract: Induced pluripotent stem cells (iPSCs) derived from a T cell of a T cell subset. T cells derived from iPSCs derived from a T cell. Methods of deriving iPSCs from a T cell. Methods of deriving T cells from iPSCs including deriving a T cell of a T cell subset from an iPSC. Methods of engineering chimeric antigen receptor (CAR)-expressing or T cell receptor (TCR)-expressing iPSC. Methods of administering T cells derived using the methods disclosed. Induced pluripotent stem cell lines derived from T cells, methods of deriving induced pluripotent stem cell lines, and methods of deriving T cells from induced pluripotent stem cell lines.
    Type: Grant
    Filed: December 8, 2016
    Date of Patent: April 8, 2025
    Assignee: REGENTS OF THE UNIVERSITY OF MINNESOTA
    Inventors: Bruce R. Blazar, Dharmeshkumar Patel, Beau R. Webber, Jakub Tolar
  • Patent number: 12269853
    Abstract: A modified human erythropoietin with an increased plasma half-life, with an erythropoietic activity of less than 0.5% in relation to a native erythropoietin, which maintains the neuroprotective and neuroplastic capacity, which comprises the mutation of at least one of the binding sites the homodimeric or heterodimeric receptor by incorporating consensus sites for N-glycosylation.
    Type: Grant
    Filed: September 26, 2019
    Date of Patent: April 8, 2025
    Assignees: UNIVERSIDAD NACIONAL DEL LITORAL, CONSEJO NACIONAL DE INVESTIGACIONES CIENTIFICAS Y TECNICAS (CONICET)
    Inventors: Marcos Oggero-Eberhardt, Maria de los Milagros Burgi-Fissolo, Aquiles Dorella, Gabriela I. Aparicio, Marina Etcheverrigaray, Camila Scorticati, Ricardo Kratje
  • Patent number: 12265093
    Abstract: An automatic analyzer includes a control unit that performs a process when a sample necessary to perform measurement for measurement content selected on an item selection screen is not arranged in a sample-container array unit. The process includes referring to the measurement content selected on the item selection screen and availability information that is information indicating whether each position in the sample-container array unit is available. A position in the sample-container array unit at which a container containing the sample necessary to perform measurement for the selected measurement content is to be arranged is specified in accordance with a specified arrangement rule, and information on the specified position in the sample-container array unit is displayed on a display unit.
    Type: Grant
    Filed: April 12, 2018
    Date of Patent: April 1, 2025
    Assignee: JEOL Ltd.
    Inventor: Yuichi Inoue
  • Patent number: 12263217
    Abstract: Chemical compositions and/or mixtures that allow nucleic acid to remain stable at ambient temperatures. The disclosed technology includes a solution and manufacturing methods thereof. The solution includes a chelating agent, a buffering agent, and a salt. The solution is configured to protect RNA and/or an RNA-based vaccine added to the solution and prevents or reduces degradation of the RNA and/or the RNA-based vaccine for a duration of 2 to 180 days over a temperature range of ?20 degrees C. to +38 degrees C. The chelating agent can comprise ethylenediaminetetraacetic acid (EDTA). The buffering agent can comprise tris(hydroxymethyl)aminomethane (TRIS). The salt can comprise NaCl. The solution is configured to preserve an injectable mRNA vaccine added to the solution, and the solution is safe for injection into mammals.
    Type: Grant
    Filed: December 3, 2021
    Date of Patent: April 1, 2025
    Assignee: Daykin Molecular Systems, LLC
    Inventors: Erik Avaniss Aghajani, John H. Phillips, Randal H. Rudderman, Robert Louis Mullen
  • Patent number: 12264322
    Abstract: The invention provides compositions and methods for reprogramming minimal volumes of mononuclear cells. In particular aspects, the invention provides methods and compositions for reprogramming minimal volumes of umbilical cord blood obtained from cord blood segments from cryopreserved cord blood segments.
    Type: Grant
    Filed: February 23, 2021
    Date of Patent: April 1, 2025
    Assignee: FATE THERAPEUTICS, INC.
    Inventors: Peter Flynn, Bahram Valamehr
  • Patent number: 12264312
    Abstract: A method for extracting nucleic acids using suitable solid supports, for example silica-based. In particular, the method includes a step of capturing nucleic acids by placing the sample in contact with a suitable solid support, characterized in that, prior to the capture step, said method includes a step of treating the sample with at least one reagent for masking the amine or carboxylic acid functional groups of the proteins and/or polysaccharides of the sample.
    Type: Grant
    Filed: March 15, 2019
    Date of Patent: April 1, 2025
    Assignee: BIOMÉRIEUX
    Inventors: Alain Laurent, Arnaud Burr, Ali Laayoun
  • Patent number: 12263220
    Abstract: The present invention provides immunoresponsive cells, including T cells, cytotoxic T cells, regulatory T cells, and Natural Killer (NK) cells, expressing at least one of an antigen recognizing receptor and one of a chimeric costimulatory receptor. Methods of using the immunoresponsive cell include those for the treatment of neoplasia and other pathologies where an increase in an antigen-specific immune response is desired.
    Type: Grant
    Filed: June 13, 2023
    Date of Patent: April 1, 2025
    Assignee: MEMORIAL SLOAN-KETTERING CANCER CENTER
    Inventors: Christopher C. Kloss, Michel Sadelain
  • Patent number: 12258578
    Abstract: Provided is an agent for accelerating maturation or aging of cells. An agent for accelerating maturation or aging of cells, comprising KU-60019 as an active ingredient.
    Type: Grant
    Filed: October 11, 2019
    Date of Patent: March 25, 2025
    Assignee: JUNTENDO EDUCATIONAL FOUNDATION
    Inventors: Wado Akamatsu, Takahiro Shiga, Naoko Kuzumaki, Hideyuki Okano
  • Patent number: 12251443
    Abstract: Polyoxazoline polymers, polymer combinations, and compositions comprising those polymers or combinations of polymers are disclosed. The invention further relates to the use of said polyoxazoline polymers, said combinations and compositions comprising such polymers and combinations for the sustained release of one or more active ingredients, in particular one or more active pharmaceutical ingredients. It further relates to the use of polyoxazoline polymers or polymer combinations as drug carrier.
    Type: Grant
    Filed: September 15, 2023
    Date of Patent: March 18, 2025
    Assignee: Universiteit Gent
    Inventors: Maarten Vergaelen, Richard Hoogenboom, Victor Retamero de la Rosa, Chris Vervaet, Aseel Samaro, Valérie Vanhoorne
  • Patent number: 12251402
    Abstract: Compositions of HLA-G+ MSC and methods of using them, including methods of transplanting compositions of HLA-G+ MSC into human recipients, are provided. Also provided are methods of preparing compositions of HLA-G+ MSC, including by treatment with DNA methylation inhibitors.
    Type: Grant
    Filed: January 23, 2020
    Date of Patent: March 18, 2025
    Assignee: Escape Therapeutics, Inc.
    Inventor: Basil M. Hantash
  • Patent number: 12246065
    Abstract: The invention provides a system that comprises pharmaceutical agents for use in immunotherapy for reducing the side-effects of an antigen-recognizing receptor against antigen-expressing non-target cells in an individual. The system includes an antigen-recognizing receptor that specifically recognizes an antigen on target cells and at least on one hematopoietic cell type in the individual. The antigen-recognizing receptor is exemplified by chimeric antigen receptors (CAR) be expressed on the surface of an immune effector cells. The system also includes hematopoietic cells resistant to recognition of the same antigen by the antigen-recognizing receptor.
    Type: Grant
    Filed: January 19, 2024
    Date of Patent: March 11, 2025
    Assignee: Miltenyi Biotec B.V. & Co. Kg
    Inventors: Michael Lutteropp, Anne Richter, Andrew Kaiser, Mario Assenmacher, Stefan Miltenyi
  • Patent number: 12247242
    Abstract: The present invention relates to a gene expression cassette including a synthetic 5? untranslated region (5? UTR), a promoter, and a regulatory gene; a recombinant vector including a replication origin and the gene expression cassette; a recombinant microorganism which has the recombinant vector introduced thereinto and shows alleviated segregational instability and; a method for preparing a recombinant microorganism having alleviated segregational instability by introducing the recombinant vector thereinto; and a method for quantitatively controlling a plasmid copy number in a recombinant microorganism.
    Type: Grant
    Filed: April 5, 2018
    Date of Patent: March 11, 2025
    Assignees: SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION, POSTECH ACADEMY-INDUSTRY FOUNDATION
    Inventors: Gyoo Yeol Jung, Sang Woo Seo, Chae Won Kang, Hyun Gyu Lim
  • Patent number: 12240882
    Abstract: The present disclosure presents a general approach to engineering existing protein-protein interactions through domain addition and evolution. The disclosure teaches the creation of novel fusion proteins that include knottin peptides where a portion of the knottin peptide is replaced with a sequence that has been created for binding to a particular target. Such fusion proteins can also be bispecific or multi specific in that they can bind to and/or inhibit two or more receptors or receptor ligands. Knottins may be fused with an existing ligand (or receptor) as a general platform tor increasing the affinity of a ligand-receptor interaction or for creating a multi specific protein. In addition, the fusion proteins may comprise a knottin peptide fused to another protein where the other protein facilitates proper expression and folding of the knottin.
    Type: Grant
    Filed: November 14, 2022
    Date of Patent: March 4, 2025
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Jennifer R. Cochran, Douglas S. Jones, Mihalis S. Kariolis, Ping-Chuan Tsai
  • Patent number: 12239670
    Abstract: The invention provides pharmaceutical compositions comprising human immature dental pulp stem cells (hIDPSCs) wherein the hIDPSCs express CD44 and CD13. The invention also provides methods of treating a neurological disease or condition comprising systemically administering to a subject a pharmaceutical composition comprising hIDPSCs wherein the hIDPSCs express CD44 and CD13. For example, for treating neurological diseases or conditions including supporting the neuro-protective mechanism in subjects diagnosed with early HD or repairing lost DA neurons in subjects diagnosed with PD.
    Type: Grant
    Filed: March 21, 2022
    Date of Patent: March 4, 2025
    Assignees: AVITA INTERNATIONAL LTD., FUNDAÃO BUTANTAN
    Inventors: Irina Kerkis, Cristiane Valverde Wenceslau
  • Patent number: 12241901
    Abstract: Systems and methods for accurate optical pH sensing of biofilms are disclosed. In one embodiment, a method of measuring an extracellular pH level using multiple wavelengths emitted by a fluorescent substance includes: exciting the fluorescent substance at an excitation wavelength; measuring a first fluorescence intensity at a first wavelength of a fluorescence emission; and measuring a second fluorescence intensity at a second wavelength of the fluorescence emission. The second wavelength is different from the first wavelength. The method also includes determining the extracellular pH level based on the first fluorescence intensity at the first wavelength and the second fluorescence intensity at the second wavelength.
    Type: Grant
    Filed: July 19, 2019
    Date of Patent: March 4, 2025
    Assignee: The University of Washington
    Inventors: Eric J. Seibel, Leonard Y. Nelson, Manuja Sharma, Jasmine Graham
  • Patent number: 12239705
    Abstract: This invention relates to dermatology and to treatments of infectious skin disease.
    Type: Grant
    Filed: April 21, 2021
    Date of Patent: March 4, 2025
    Assignee: University of Rochester
    Inventors: Matthew G. Brewer, Lisa A. Beck, Brian M. Ward, Benjamin L. Miller
  • Patent number: 12233417
    Abstract: Provided is a microfluidic chip for physicochemically treating a single cell whose diameter is 50-400 micrometers.
    Type: Grant
    Filed: July 16, 2018
    Date of Patent: February 25, 2025
    Assignee: Shenzhen Vitavitro Biotech Co., Ltd.
    Inventors: Xiaozhen Lin, Jie Wu, Yanjing Fang
  • Patent number: 12234288
    Abstract: The present invention relates to novel polypeptides, which are derived from Arginase2. The invention also concerns uses of the polypeptides and compositions comprising the polypeptides.
    Type: Grant
    Filed: November 14, 2019
    Date of Patent: February 25, 2025
    Assignee: IO Biotech ApS
    Inventor: Mads Hald Andersen
  • Patent number: 12236503
    Abstract: The present invention discloses a PET system attenuation correction method based on a flow model. The flow model adopted is a completely reversible model, the forward and reverse mappings share the same parameters, and the structure itself is a consistency constraint. The model utilizes the spatial correlation of adjacent slices, and adopts the structure of multi-slice input and single-slice output. The model consists of multiple reversible blocks, each of which consists of a enhanced affine coupling layer and a reversible 1×1 convolutional layer, and uses several small u-nets to learn the transformation parameters of the enhanced affine coupling layer. The present invention avoids additional CT or MR scanning, saves scanning cost for the patient, and reduces the damage of CT radiation to the patient; compared with similar methods, higher quality non-attenuation-corrected PET image can be obtained.
    Type: Grant
    Filed: April 2, 2022
    Date of Patent: February 25, 2025
    Assignee: ZHEJIANG UNIVERSITY
    Inventors: Huafeng Liu, Bo Wang
  • Patent number: 12227725
    Abstract: A cell culturing method and device that uses a cell culture bag (1) that includes a bag body (2) configured from an upper surface film (21) and a lower surface film (22) having a sealed periphery, and a port (3) attached to the bag body (2), and a plurality of recesses (4) being formed in the lower surface film (22). The method including: a closing-off step for closing off the plurality of recesses (4) using the upper surface film (21) by discharging a culture medium (S) contained in the bag body (2) through the port (3); and a releasing step for releasing, in some or all of the plurality of closed-off recesses (4), cells or cell aggregates adhering to the inner surfaces of the recesses (4).
    Type: Grant
    Filed: January 4, 2019
    Date of Patent: February 18, 2025
    Assignee: TOYO SEIKAN GROUP HOLDINGS, LTD.
    Inventors: Ryo Suenaga, Maki Mitsuishi
  • Patent number: 12226427
    Abstract: Methods of reactivating transcription of a fragile X mental retardation 1 (FMR1) gene and treating FMR1-associated diseases are provided. Compositions and kits for doing same are also provided.
    Type: Grant
    Filed: August 15, 2019
    Date of Patent: February 18, 2025
    Assignee: YISSUM RESEARCH DEVELOPMENT COMPANY OF THE HEBREW UNIVERSITY OF JERUSALEM LTD.
    Inventors: Nissim Benvenisty, Dan Vershkov
  • Patent number: 12215352
    Abstract: The present disclosure relates to a cell culture medium that promotes cell proliferation during serum-free cell culture.
    Type: Grant
    Filed: March 21, 2023
    Date of Patent: February 4, 2025
    Assignee: MESOBLAST INTERNATIONAL SARL
    Inventors: Paul Simmons, Colby Suire
  • Patent number: 12215346
    Abstract: The present invention relates to a method for isolating and culturing neural stem cells with high efficiency, which may shorten the time for isolation and culture by simplifying a method for isolating and culturing neural stem cells and may increase the acquisition yield of neural stem cells. The present invention provides a method for isolating and culturing neural stem cells with high efficiency, comprising the steps of adding brain tissue into an enzyme solution so as to subject the brain tissue to enzyme treatment; physically isolating cell clumps from the enzyme treated brain tissue by dividing the cell clumps according to size and removing impurities; and inoculating the cell clumps on a culture dish so as to subculture.
    Type: Grant
    Filed: January 30, 2019
    Date of Patent: February 4, 2025
    Assignee: MEDINNO INC.
    Inventors: Kyeung Min Joo, Hyun Nam
  • Patent number: 12215355
    Abstract: Provided herein are methods of producing ? cells and precursors thereof utilizing a Wnt signaling inhibitor or PKC activator, or both. Also provided herein are in vitro cultures comprising said cells, methods of treating a subject with a disease characterized by high blood sugar levels over a prolonged period of time by administering said cells, and devices for encapsulating said cells.
    Type: Grant
    Filed: February 9, 2021
    Date of Patent: February 4, 2025
    Assignee: Vertex Pharmaceuticals Incorporated
    Inventors: Felicia J. Pagliuca, George Harb, Lillian Ye
  • Patent number: 12201509
    Abstract: This disclosure describes decellularized, biologically-engineered tubular grafts and methods of making and using such decellularized, biologically-engineered tubular grafts.
    Type: Grant
    Filed: March 6, 2023
    Date of Patent: January 21, 2025
    Assignee: Regents of the University of Minnesota
    Inventors: Robert Tranquillo, Zeeshan Syedain, Lee Meier
  • Patent number: 12195764
    Abstract: According to the present disclosure, there is provided a method for culturing cells into which a reprogramming factor is introduced including culturing cells into which a reprogramming factor is introduced; and recovering all cells into which the reprogramming factor is introduced and seeding and passaging at least part of the recovered cells in a medium. In addition, there is provided a method for culturing cells into which a reprogramming factor is introduced, including culturing cells into which a reprogramming factor is introduced; and inducing somatic cells different from pluripotent stem cells without passaging the cells into which the reprogramming factor is introduced.
    Type: Grant
    Filed: December 29, 2021
    Date of Patent: January 14, 2025
    Assignee: I Peace, Inc.
    Inventors: Koji Tanabe, Kenta Suto
  • Patent number: 12195774
    Abstract: The present disclosure relates to antidotes to anticoagulants targeting factor Xa. The antidotes are factor Xa protein derivatives that bind to the factor Xa inhibitors thereby substantially neutralizing them but do not assemble into the prothrombinase complex. In one embodiment, the derivatives described herein lack or have reduced intrinsic coagulant activity.
    Type: Grant
    Filed: June 18, 2019
    Date of Patent: January 14, 2025
    Assignee: Alexion Pharmaceuticals, Inc.
    Inventors: Pamela B. Conley, Genmin Lu, Leonard G. Presta, John T. Curnutte
  • Patent number: 12195768
    Abstract: A system and method for modulating stress granule assembly, utilizing a protein construct that has a cell penetrating protein fused to one or more proteins that can bind with an NTF2-like domain of a G3BP protein. By configuring the protein construct with an appropriate number of proteins that being with NTF2-like domains, stress granule assembly can be upregulated or downregulated as needed to treat patients.
    Type: Grant
    Filed: July 12, 2019
    Date of Patent: January 14, 2025
    Assignee: THE TRUSTEES OF PRINCETON UNIVERSITY
    Inventors: Cliff Brangwynne, Victoria Drake, David Sanders
  • Patent number: 12194059
    Abstract: In one aspect, methods of promoting bone growth are described herein. In some embodiments, a method of promoting bone growth described herein comprises promoting cell differentiation or phenotype progression in a population of bone cells by providing a citrate-presenting composition to the population of bone cells. In some embodiments, the citrate-presenting composition is provided to the bone cells at a first stage of cell development selected to obtain a first cell differentiation or phenotype progression. Additionally, in some cases, a second citrate-presenting composition is further provided to the bone cells at a second stage of cell development selected to obtain a second cell differentiation or phenotype progression.
    Type: Grant
    Filed: March 10, 2022
    Date of Patent: January 14, 2025
    Assignee: Board of Regents, The University of Texas System
    Inventor: Jian Yang
  • Patent number: 12195513
    Abstract: Provided are chimeric antigen receptors having the hinge, transmembrane region, and/or intracellular domain of LILRB1, or functional fragments or variants thereof. Also provided herein are cells comprising the LILRB1 based receptors, and methods of making and using same.
    Type: Grant
    Filed: January 31, 2022
    Date of Patent: January 14, 2025
    Assignee: A2 Biotherapeutics, Inc.
    Inventors: Carl Alexander Kamb, Agnes E. Hamburger, Breanna Diandreth, Mark E. Daris, Kiran Deshmukh
  • Patent number: 12187775
    Abstract: Provided are chimeric antigen receptors having the hinge, transmembrane region, and/or intracellular domain of LILRB1, or functional fragments or variants thereof. Also provided herein are cells comprising the LILRB1 based receptors, and methods of making and using same.
    Type: Grant
    Filed: January 31, 2022
    Date of Patent: January 7, 2025
    Assignee: A2 Biotherapeutics, Inc.
    Inventors: Carl Alexander Kamb, Agnes E. Hamburger, Breanna Diandreth, Mark E. Daris, Kiran Deshmukh
  • Patent number: 12188932
    Abstract: The present invention relates to a method for high throughput screening for a TCR-binding peptide ligand/MHC molecule complex, comprising a stabilized peptide-MHC molecule and respective uses of said method. The present invention further relates to polypeptides comprising or consisting of stabilized MHC molecules or peptide binding fragments thereof, pharmaceutical compositions comprising said polypeptides, vaccines comprising said pharmaceutical composition and uses of said vaccine for the manufacturing of a medicament and/or in the prevention of cancer The present invention further relates to nucleic acids encoding said polypeptides and vectors comprising said nucleic acids.
    Type: Grant
    Filed: September 13, 2019
    Date of Patent: January 7, 2025
    Assignee: Immatics Biotechnologies GmbH
    Inventors: Andreas Moritz, Dominik Maurer, Sebastian Bunk, Claudia Wagner
  • Patent number: 12187997
    Abstract: The invention provides integrated Organ-on-Chip microphysiological systems representations of living Organs and support structures for such microphysiological systems.
    Type: Grant
    Filed: June 30, 2023
    Date of Patent: January 7, 2025
    Assignee: President and Fellows of Harvard College
    Inventors: Donald E. Ingber, Anthony M. Bahinski, Robert Cunningham, Josue A. Goss, Geraldine A. Hamilton, Christopher David Hinojosa, Daniel Levner, Kevin Kit Parker
  • Patent number: 12180457
    Abstract: Some embodiments provided herein relate to methods and compositions for making genetically modified T cells. In some such embodiments, CD4+ and CD8+ T cells are cultured in a single serum-free volume. In some embodiments, co-cultured CD4+ and CD8+ T cells can be transduced with a lentiviral vector, and a population of transduced T cells can be harvested within a shorter period of time than other conventional methods.
    Type: Grant
    Filed: February 4, 2019
    Date of Patent: December 31, 2024
    Assignee: Seattle Children's Hospital
    Inventors: Michael C. Jensen, Joshua Gustafson
  • Patent number: 12180260
    Abstract: The present invention relates to T cell receptors (TCRs) which bind the HLA-A*0201 restricted peptide GVYDGREHTV (SEQ ID NO: 1) derived from the MAGE-A4 protein. The TCRs of the invention demonstrate excellent specificity profiles for this MAGE epitope. Also provided are nucleic acids encoding the TCRs, cells engineered to present the TCRs, cells harbouring expression vectors encoding the TCRs and pharmaceutical compositions comprising the TCRs, nucleic acids or cells of the invention.
    Type: Grant
    Filed: June 22, 2023
    Date of Patent: December 31, 2024
    Assignee: Adaptimmune Limited
    Inventors: Nicholas Tribble, William Lawrance, Eleanor Bagg
  • Patent number: 12180261
    Abstract: The present invention relates to T cell receptors (TCRs) which bind the HLA-A*0201 restricted peptide GVYDGEEHSV (SEQ ID NO: 1) derived from the MAGE-B2 protein. The TCRs of the invention demonstrate excellent specificity profiles for this MAGE epitope. Also provided are nucleic acids encoding the TCRs, cells engineered to present the TCRs, cells harbouring expression vectors encoding the TCRs and pharmaceutical compositions comprising the TCRs, nucleic acids or cells of the invention.
    Type: Grant
    Filed: August 1, 2023
    Date of Patent: December 31, 2024
    Assignee: Adaptimmune Limited
    Inventors: Nicholas Tribble, William Lawrance, Eleanor Bagg
  • Patent number: 12173077
    Abstract: The present invention is directed to a humanized BCMA antibody or an antigen-binding fragment thereof, comprising VH having the amino acid sequence of SEQ ID NO: 3 and VL having the amino acid sequence of SEQ ID NO: 5. The present invention is also directed to a BCMA chimeric antigen receptor fusion protein comprising from N-terminus to C-terminus: (i) a single-chain variable fragment (scFv) of the present invention, (ii) a transmembrane domain, (iii) at least one co-stimulatory domains, and (iv) an activating domain. This humanized BCMA-CAR-T cells have specific killing activity against BCMA-positive tumor cells.
    Type: Grant
    Filed: May 31, 2023
    Date of Patent: December 24, 2024
    Assignees: ProMab Biotechnologies, Inc., Forevertek Biotechnology Co., Ltd
    Inventors: Lijun Wu, Vita Golubovskaya
  • Patent number: 12171785
    Abstract: The invention provides isolated stem cells of non-embryonic origin that can be maintained in culture in the undifferentiated state or differentiated to form cells of multiple tissue types. Also provided are methods of isolation and culture, as well as therapeutic uses for the isolated cells.
    Type: Grant
    Filed: June 8, 2015
    Date of Patent: December 24, 2024
    Assignees: Regents of the University of Minnesota, HEALIOS K.K.
    Inventors: Leo T. Furcht, Catherine M. Verfaillie, Morayma Reyes
  • Patent number: 12171809
    Abstract: The present invention provides a composition for delivering a polynucleotide into cells via a polyplex. The polyplex comprises the polynucleotide and a polymer. The composition comprises the polyplex, a collagen-mimetic peptide (CMP) and collagen fragments. The CMP is bound to the polyplex and the collagen fragments. Also provided are the uses of the composition in methods of delivering a polynucleotide into cells as well as methods of improving wound healing in a subject, enhancing cell proliferation in a subject, enhancing production of extracellular matrix by cells in a subject and/or enhancing cell migration by cells in a subject.
    Type: Grant
    Filed: July 18, 2017
    Date of Patent: December 24, 2024
    Assignee: UNIVERSITY OF DELAWARE
    Inventors: Kristi Kiick, Millicent Sullivan, Morgan Urello
  • Patent number: 12168779
    Abstract: A method and related apparatus for confirming whether a kill laser successfully destroys an undesired population of cells includes introducing fluorescent dye into cells, exciting the cells with a detection laser or a light emitting diode to cause the cell to fluoresce for a first time, measuring the amount of fluorescence in the cells with a detector capable of emitting a detection pulse, classifying the cells via embedded processing as undesired or desired cells based on the amount of fluorescence, firing a kill beam with a kill laser at any undesired cells, measuring the amount of fluorescence in the cells a second time to determine whether a fluorescent event was generated from the kill beam striking the cells, and providing feedback to an operator of the kill laser as to whether any fluorescent events were generated from the kill beam striking the cells.
    Type: Grant
    Filed: July 14, 2023
    Date of Patent: December 17, 2024
    Assignee: ABS Global, Inc.
    Inventors: Frederick Savage, David Appleyard, Zheng Xia, Matthew D. Ebersole, Daniel McAda
  • Patent number: 12161696
    Abstract: The present disclosure provides methods treating reversible hemophilic arthropathy of a joint in a human having hemophilia comprising administering to the human an effective amount of a chimeric protein or composition comprising a clotting factor and an Fc region.
    Type: Grant
    Filed: December 1, 2017
    Date of Patent: December 10, 2024
    Assignee: BIOVERATIV THERAPEUTICS INC.
    Inventors: Jennifer Dumont, Nisha Jain, Desilu Glazebrook