Cabbages treated to control post-harvest diseases

Growth of organisms of the post-harvest disease causing fungi Botrytis cinerea and Alternaria brassicicola is inhibited by applying to vegetables of the Brassica family, particularly cabbage, isolates of bacteria of species Pseudomonas fluorescens, Serratia liquefaciens, Serratia plymuthica, Bacillus subtilis, Bacillus pumilis and Bacillus polymyxa.

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Claims

1. A cabbage having applied thereto an isolated bacteria having the identifying characteristics of an isolate selected from the group consisting of Bacillus pumilus NCIMB 40489, Pseudomonas fluorescens NCIMB 40490, Bacillus subtillis NCIMB 40491, Serratia liquefaciens NCIMB 40492, Serratia plymuthica NCIMB 40493, Pseudomonas fluorescens NCIMB 40495, and Pseudomonas fluorescens NCIMB 40497 including the characteristic of being capable of inhibiting growth of fungi of species Alternaria brassicicola on homogenized cabbage tissue:agar:water mixtures.

2. A cabbage according to claim 1 wherein the isolated bacteria has the identifying characteristics of an isolate selected from the group consisting of Pseudomonas fluorescens NCIMB 40490, Serratia liquefaciens NCIMB 40492, Serratia plymuthica NCIMB 40493, Pseudomonas fluorescens NCIMB 40495, and Pseudomonas fluorescens NCIMB 40497 said isolate being capable of inhibiting growth of Alternaria brassicicola on cabbage leaves at temperatures below 20.degree. C.

3. A cabbage according to claim 1 wherein the isolated bacteria is selected from the group consisting of Psuedomonas fluorescens, Serratia plymuthica and Serratia liquefaciens.

4. A cabbage according to claim 1 wherein the isolated bacteria has identifying characteristics of an isolate selected from the group consisting of Bacillus pumilus NCIMB 40489 and Bacillus subtilis NCIMB 40491, said isolate being capable of inhibiting growth of Alternaria brassicicola on cabbage leaves at temperatures of about 20.degree. C.

6. A cabbage according to claim 5 wherein the isolated bacteria has the identifying characteristics of an isolated selected from the group consisting of, Pseudomonas fluorescens NCIMB 40490, Serratia liquefaciens NCIMB 40492, Serratia plymuthica NCIMB 40493, Pseudomonas fluorescens NCIMB 40495, and Pseudomonas fluorescens NCIMB 40497 said isolated being capable of inhibiting growth of Botrytis cinerea on cabbage leaves at temperatures below 20.degree. C.

7. A cabbage according to claim 5 wherein the isolated bacteria is selected from the group consisting of Psuedomonas fluorescens, Serratia plymuthica and Serratia liquefaciens.

8. A cabbage according to claim 5 wherein the isolated bacteria has identifying characteristics of an isolate selected from the group consisting of Bacillus pumilus NCIMB 40489 and Bacillus subtilis NCIMB 40491, said isolate being capable of inhibiting growth of Botrytis cinerea on cabbage leaves at temperatures of about 20.degree. C.

Referenced Cited
U.S. Patent Documents
4663162 May 5, 1987 Kado et al.
4764371 August 16, 1988 Pusey et al.
5194269 March 16, 1993 Lee
5371011 December 6, 1994 Bernier et al.
Foreign Patent Documents
0 276 132 July 1988 EPX
0 376 775 July 1990 EPX
Other references
  • Blakeman et al Microbiology of Aerial Plant Surfaces 1976 pp. 528-557 "Inhibition of Pathogens by Epiphytic Bacteria on Aerial Plant Surfaces". Danby et al LUMS, 45 PIPS No. 47822 pp. 405-407 "Activity of antibiotics produced by Bacillus subtilis . . . ". Agrics. G.N. Plant Pathology 1988 pp. 520-523 "Bacterial Soft spot and blights bacterialRots" pp. 552-553. Brenner, Bedgey's Manual of Systematic Bacteriology, 9th ed. pp. 408-421 Faculatively Anaerobic Gram etc. Wale et al Proceedings of 7th Long Ashton Symposium Apr. 1979 pp. 301-312 "Quality in Stored and Processed . . . ". Wale Post-Harvest Pathology of Dutch White Cabbage 1980 Summary pp. vii-viii. J. Argic. Food Chem. 1988. 36, 366-370 Cueldner et al "Isolation and Identificaiton of Iturins . . . ". Bacteriological Reviews 6i77 vol. 41 No. 2 pp. 449-474 Katz et al "The Peptide Antibiotics of Bacillus . . . ". Can. J. Microbiol. vol. 32, 1986 pp. 963-967 Utkhede et al "In vitro Inhibition of plant pathogens . . . ". Can. J. Bot. vol. 67, 1989 pp. 2317-2323 Wisniewski et al "Characterization of Inhibition of Rhizopus . . . ". Annu. Rev. Phytopathol. 1989, 27:425-41 Wilson et al "Biological Control of Postharvest Diseases . . . ". Plant Diseases vol. 70 No. 2 Feb. 1986 pp. 107-109 Spotts et al "Populations, Pathogenicity, and Benomyl . . . ". Phytopathology vol. 78 No. 8, 1988 pp. 1075-1079 Howell et al "Production of Ammonia by Enterobacter . . . ". Phytopathology vol. 78, No. 12, 1988 pp. 1697-1701 Janisiewicz et al "Biological control of Blue Mold . . . ". Plant Disease vol. 66, No. 8 pp. 703-707 Colyner "Bacterization of Potatoes with Pseudomonas . . . ". Plant Disease vol. 69 No. 9 pp. 770-773 Baker et al "Biocontrol of Bean Rust by Bacilllyus subilis . . . ". Phytopathology vol. 77, No. 3, 1987 pp. 481-485 Janisiewicz "Post Harvest Biological Control of Blue Mold . . . ". Ann. appl. Biol. 1975, 81 pp. 399-408 Robinson et al "Storage Characteristics of some vegetables . . . ". Trans Br. mycol. Soc. 83(3), 487-490 1984 Singh et al "Bacillus subtilis as a Control Agent Against . . . ". Appl. & Environmental Microbiology Nov. 1986 pp. 1183-1189 James et al "Multiple Antibiotics Produced . . . ". Antimicrobial Agents and Chemotherapy Mar. 1986 488-495 Gurusiddaiah et al "Characterization of an . . . ". Phytopathology, vol. 76, No. 2, 1986 pp. 136-139 McKeen et al. "Production and Partial . . . ". Plant Disease vol. 72, No. 7 Pusey et al "Pilot tests for Commercial production . . . ". Plant Disease Sep. 1984 pp. 753-757 Pusey et al "Postharvest Biological Control of stone . . . ".
Patent History
Patent number: 5780080
Type: Grant
Filed: Mar 27, 1995
Date of Patent: Jul 14, 1998
Assignee: The Ministry of Agriculture, Fisheries and Food in Her Britannic Majesty's Government of the U.K. of Gt. Britian & N. Ireland
Inventors: Carlo Leifert (Aberden), Harold A. S. Epton (Cheadle), David C. Sigee (Sale)
Primary Examiner: Leslie Wong
Law Firm: Nixon & Vanderhye
Application Number: 8/411,286