Human p24 vesicle proteins
The present invention provides two human p24 vesicle trafficking proteins (designated individually as Hp24-1 and Hp24-2 and collectively as Hp24) and polynucleotides which identify and encode Hp24. The invention also provides genetically engineered expression vectors and host cells comprising the nucleic acid sequences encoding Hp24 and a method for producing Hp24. The invention also provides for agonists, antibodies, or antagonists specifically binding Hp24, and their use, in the prevention and treatment of diseases associated with expression of Hp24. Additionally, the invention provides for the use of antisense molecules to polynucleotides encoding Hp24 for the treatment of diseases associated with the expression of Hp24. The invention also provides diagnostic assays which utilize the polynucleotide, or fragments or the complement thereof, and antibodies specifically binding Hp24.
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Claims
1. An isolated and purified polynucleotide sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:1.
2. A hybridization probe consisting of the polynucleotide sequence of claim 1 and a detectable label.
3. An isolated and purified polynucleotide sequence comprising SEQ ID NO:2.
4. An isolated and purified polynucleotide sequence which is completely complementary to the polynucleotide sequence of claim 1.
5. A hybridization probe consisting of the isolated and purified polynucleotide sequence of claim 4 and a detectable label.
6. An expression vector containing the polynucleotide sequence of claim 1.
7. A host cell containing the expression vector of claim 6.
8. A method for producing a polypeptide comprising the amino acid sequence of SEQ ID NO:1, the method comprising the steps of:
- a) culturing the host cell of claim 7 under conditions suitable for the expression of the polypeptide; and
- b) recovering the polypeptide from the host cell culture.
9. An isolated and purified polynucleotide sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:3.
10. A hybridization probe consisting of the polynucleotide sequence of claim 9 and a detectable label.
11. An isolated and purified polynucleotide sequence comprising SEQ ID NO:4.
12. An isolated and purified polynucleotide sequence which is completely complementary to the isolated and purifiedbpolynucleotide sequence of claim 9.
13. A hybridization probe consisting of the polynucleotide sequence of claim 12 and a detectable label.
14. An expression vector containing the polynucleotide sequence of claim 9.
15. A host cell containing the expression vector of claim 4.
16. A method for producing a polypeptide comprising the amino acid sequence of SEQ ID NO:3, the method comprising the steps of:
- a) culturing the host cell of claim 15 under conditions suitable for the expression of the polypeptide; and
- b) recovering the polypeptide from the host cell culture.
- "Biochemistry" Lehninger Worth Publishers, NY 1975 Ch. 34, see especially p. 961. Hillier, et al., "The WashU-Merck EST Project," NCBI, Accession No. AA167650, Dec. 19, 1996. Hillier, et al., "The WashU-Merck EST Profect," NCBI, Accession No. N39817, Jan. 22, 1991. Hillier, et al., "The WashU-NCI Human EST Project," NCBI, Accession No. AA479763, Nov. 9 1997. National Cancer Institute, "Cancer Genome Anatomy Project," NCBI, Accession No. AA469202, Aug. 13, 1997. Rothman, J.E., "Mechanisms of intracellular protein transport," Nature, 372:55-63 (1994). Stamnes, M., et al., "An integral membrane component of coatomer-coated transport vesicles defines a family of proteins involved in budding," Proc. Natl. Acad. Sci. USA, 92:8011-8015 (1995). Fiedler, K., et al., "Bimodal Interaction of Coatomer with the p24 Family of Putative Cargo Receptors," Science, 273:1396-1399 (1996). Schimmoller, F., et al., "The absence of Emp24p, a component of ER-derived COPII-coated vesicles, causes a defect in transport of selected proteins to the Golgi," EMBO J., 14(7):1329-1339 (1995). Rothman, J., et al., "Protein Sorting by Transport Vesicles," Science, 272:227-234 (1996). Blum, R., et al., "Tmp21 and p24A, Two Type I Proteins Enriched in Pancreatic Microsomal Membranes, Are Members of a Protein Family Involved in Vesicular Trafficking," J. Biol. Chem., 271(29):17183-17189 (1996). Gayle, M., et al., "Cloning of a Putative Ligand for the T1/ST2 Receptor," J. Biol. Chem., 271(10):5784-5789 (1996).
Type: Grant
Filed: Feb 14, 1997
Date of Patent: Feb 9, 1999
Assignee: Incyte Pharmaceuticals, Inc. (Palo Alto, CA)
Inventors: Surya K. Goli (Sunnyvale, CA), Olga Bandman (Mountain View, CA)
Primary Examiner: Toni R. Scheiner
Assistant Examiner: Nancy A. Johnson
Attorneys: Leanne C. Price, Lucy J. Incyte Pharmaceuticals, Inc. Billings
Application Number: 8/801,740
International Classification: C07H 2104; C12N 1563; C12N 121;
