Patents Issued in February 26, 2008
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Patent number: 7335468Abstract: Isolated protein complexes are provided comprising Tsg101 and HIV GAG or GAGp6. The protein complexes are useful in screening assays for selecting compounds effective in modulating the Tsg101-HIV GAG or GAGp6 interaction within the protein complexes.Type: GrantFiled: September 15, 2003Date of Patent: February 26, 2008Assignee: Myriad Genetics, Inc.Inventors: Kenton Zavitz, Scott Morham, Daniel Albert Wettstein
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Patent number: 7335469Abstract: Diagnostic and therapeutic applications for Noonan Syndrome are described. The diagnostic and therapeutic applications are based on certain mutations in the protein tyrosine phosphatase gene PTPN11 and its expression product, PTPN11, as well as mutations in other components in a PTPN11 signal transduction pathway promoting an increased signaling flux. Also described are nucleotide sequences, amino acid sequences, probes, and primers related to PTPN11 and PTPN11 variants, and cells expressing such variants.Type: GrantFiled: October 1, 2002Date of Patent: February 26, 2008Assignee: Mt. Sinai School of Medicine of New York UniversityInventors: Bruce D. Gelb, Marco Tartaglia
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Patent number: 7335470Abstract: In one aspect the invention provides compilations of nucleic acids, articles of manufacture, e.g., arrays, and methods for the detection of chromosomal abnormalities, such as a chromosomal aneuploidies, deletions, amplifications, and the like, and the diagnosis or prognosis of syndromes associated with a contiguous gene abnormality. Kits are also provided.Type: GrantFiled: October 15, 2002Date of Patent: February 26, 2008Assignee: PerkinElmer, LAS, Inc.Inventors: Mansoor Mohammed, Jason Kang, Shishir Shah, Wei-Wen Cai
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Patent number: 7335471Abstract: Mutant RNA polymerases of phagic origin in which the peptide chain is modified by substitution, deletion or addition of at least one amino acid, the modification having the effect of reducing the sensitivity of the RNA polymerases to the initial transcription sequence of the DNA sequence coding for the RNA, for a method for production of the RNA, or proteins coded by the RNA, from given nucleotide sequences, comprising a sequence of DNA coding for the RNA, the transcription of which is placed under the control of a promoter recognised by wild-type RNA polymerases and the mutant RNA polymerases as above. The method has a higher yield of RNA than the yield obtained when using the wild-type RNA polymerases in the presence of the same non-consensual ITS as that found in the sequence of DNA coding for the RNA.Type: GrantFiled: March 19, 2002Date of Patent: February 26, 2008Assignee: Centre National de la Recherche ScientifiqueInventors: Jean Guillerez, Pascal Lopez, Marc Dreyfus
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Patent number: 7335472Abstract: The present invention relates to a method for simultaneously detecting hybridization reactions and immunoreactions in a sample which may contain target analytes consisting of at least one nucleic acid and of at least one other ligand that is different in nature, characterized in that it comprises the steps consisting in: (i) depositing a known amount of volume of the sample diluted in a reaction buffer, onto a capture surface pre-coated with the partners for capturing said target analytes, said capture partners consisting of at least one nucleic acid probe and at least one antiligand, (ii) reacting at a temperature of between 15° C. and 60° C. and (iii) visualizing the hybridization reactions and immunoreactions thus obtained, and also to the use of this method for detecting diseases of infectious or metabolic origin or viral origin, for the industrial diagnosis of the presence of bacteria, and for identifying and/or quantifying biological molecules.Type: GrantFiled: June 3, 2003Date of Patent: February 26, 2008Assignee: BiomerieuxInventors: Bernard Mandrand, Agnès Perrin, Alain Theretz
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Patent number: 7335473Abstract: Composition of matter comprising the causative agent of Mystery Swine Disease, Lelystad Agent, in a live, attenuated, dead, or recombinant form, or a part or component of it. Vaccine compositions and diagnostic kits based thereon. Recombinant nucleic acid comprising a Lelystad Agent-specific nucleotide sequence. Peptides comprising a Lelystad Agent-specific amino acid sequence. Lelystad Agent-specific antibodies.Type: GrantFiled: July 14, 2004Date of Patent: February 26, 2008Assignee: Boehringer Ingelheim Vetmedica GmbHInventors: Gert Wensvoort, Catharinus Terpstra, Joannes Maria Anthonis Pol, Robertus Jacobus Maria Moormann, Johanna Jacoba Maria Meulenberg
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Patent number: 7335474Abstract: Methods and systems for identifying biological marker-placebo effect correlations are provided. Clinical trial design and data analysis of clinical trial data is modified to accommodate marker-placebo effect correlations.Type: GrantFiled: September 13, 2004Date of Patent: February 26, 2008Assignee: Perlegen Sciences, Inc.Inventors: Bradley Margus, David Cox, Jesse Hsu
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Patent number: 7335475Abstract: The present invention provides an isolated nucleic acid molecule encoding a PFM/SET polypeptide. Also provided is an isolated nucleic acid molecule encoding a functional fragment of a PFM/SET polypeptide that contains a PR, SET, PRAZ or PKZL domain of a PFM/SET polypeptide of the invention. Further provided by the invention are PFM/SET polypeptides, and functional fragments thereof that contain a PR, SET, PRAZ or PKZL domain of a PFM/SET polypeptide. The invention also provides PFM/SET antibodies, PFM/SET modulatory compounds, and related methods. The molecules of the invention can be used in methods of screening for a compound that modulates PFM/SET polypeptide histone methyltransferase activity and to modulate cell proliferation to prevent or treat proliferative disorders, including cancer. Additionally, the molecules and methods of the invention can be used to diagnose and prognose proliferative disorders.Type: GrantFiled: May 3, 2005Date of Patent: February 26, 2008Assignee: The Burnham InstituteInventor: Shi Huang
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Patent number: 7335476Abstract: The present invention relates to fatty acid desaturases and elongases able to catalyze the conversion of linoleic acid (LA) to ?-linolenic acid (GLA); ?-linoleic acid (ALA) to stearidonic acid (STA); GLA to dihomo-?-linoleic acid (DGLA); STA to eicosatetraenoic acid (ETA); DGLA to ETA; eicosapentaenoic acid (EPA) to docosapentaenoic acid (DPA); and arachidonic acid (ARA) to EPA. Nucleic acid sequences encoding codon-optimized desaturases and elongases, nucleic acid sequences which hybridize thereto, DNA constructs comprising the codon-optimized desaturase or elongases, and recombinant host microorganisms expressing increased levels of desaturase or elongase are described.Type: GrantFiled: August 4, 2006Date of Patent: February 26, 2008Assignee: E.I. du Pont de Nemours and CompanyInventors: Stephen K. Picataggio, Quinn Qun Zhu
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Patent number: 7335477Abstract: A recombinant, refolded non-fusion polypeptide expressed from a truncated r56 gene of the causative agent of scrub typhus, Orientia tsutsugamushi for the Karp, Kato and Gilliam strains has been produced. The invention is useful for detecting prior exposure to scrub typhus, screening for and/or identification of at least one infectious strain-similarity (i.e. a Karp-like, Kato-like or Gilliam-like strain) based on its strength of reaction toward a truncated protein and as a component in vaccine formulations and production of immune globulins for passive prophylaxis and immunity in subjects.Type: GrantFiled: April 12, 2002Date of Patent: February 26, 2008Assignee: The United States of America as represented by the Secretary of the NavyInventors: Wei-Mei Ching, Daryl J. Kelly, Gregory A. Dasch
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Patent number: 7335478Abstract: This invention provides methods and systems for linking the functional activation of a responder molecule to the interaction of two or more binding ensemble members of interest either in vitro or in vivo, and thereby producing a signal, phenotype, or other functional output in response to the interaction of the binding ensemble members. The systems comprise a responder, an inhibitor of the responder, and an inhibitor of the inhibitor, or “reactivator” of the responder, and interacting components. Two binding ensemble members are complexed to the other components of the system in such a way that interaction of the binding ensemble members, either directly or via additional ensemble members, causes a shift in the equilibrium of inhibitor binding from the responder to the reactivator, thereby functionally activating the responder.Type: GrantFiled: July 29, 2002Date of Patent: February 26, 2008Assignee: KaloBios Pharmaceuticals, Inc.Inventors: Robert F. Balint, Jeng-Horng Her
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Patent number: 7335479Abstract: The invention identifies nucleic acid and amino acid sequences of a sensory cell specific G-protein alpha subunit that are specifically expressed in sensory cells, e.g., taste cells, antibodies to such G-protein alpha subunits, methods of detecting such nucleic acids and subunits, and methods of screening for modulators of a sensory cell specific G-protein alpha subunit.Type: GrantFiled: March 18, 2005Date of Patent: February 26, 2008Assignee: The Regents of the University of CaliforniaInventor: Charles S. Zuker
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Patent number: 7335480Abstract: The disclosed technology provides an enriched antibody population, highly specific for an antigen of a surface polysaccharide, from a mycobacterium. In a related embodiment, the antibody is enriched by having been raised in an environment that maintains antigenically active antigen. These antibodies may be used in an immunoreactive environment for detecting the presence of a mycobacterial infection in a sample from a subject.Type: GrantFiled: July 20, 2005Date of Patent: February 26, 2008Assignee: Chemogen, Inc.Inventors: Vladimir A. Koulchin, Elena V. Molokova, Jill L. Kerrick
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Patent number: 7335481Abstract: The present invention provides methods for screening and identifying compounds that affect the metabolism of fatty acids and fatty acid derivatives, and thus for compounds that possess anti-diabetic as well as anti-obesity properties and possess the ability to affect the levels of chylomicrons, triacylglycerols, cholesterols, and fatty acids in a patient. Kits and compositions for screening and identifying such compounds are also provided. The invention is predicated on the identification of a physiological receptor for free fatty acids and anti-diabetic and anti-obesity drugs.Type: GrantFiled: July 24, 2002Date of Patent: February 26, 2008Inventors: Christer Owman, Bjorn Olde, Knut Kotarsky, Niclas Nilsson, Erik Flodgren
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Patent number: 7335482Abstract: A novel amino acid transporter molecule mediating transportation of amino acids, which are nutrients essentially required in the survival and proliferation of various normal cells constituting a living body and various pathology-associated abnormal cells such as tumor cells, into cells and being expressed specifically in tumor cells compared with normal cells; and drugs for treating various pathogenic conditions such as tumor (cancer) which are obtained by identifying and isolating the above amino acid transporter molecule and identifying a substance capable of inhibiting the biological activity and/or expression of this molecule. Intensive studies were made to identify a tumor cell membrane surface molecule associating or interacting with a cell membrane surface 4F2hc molecule seemingly playing an important role in the activation of an unknown amino acid transporter.Type: GrantFiled: August 5, 2004Date of Patent: February 26, 2008Assignee: Japan Science and Technology CorporationInventors: Hitoshi Endou, Yoshikatsu Kanai
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Patent number: 7335483Abstract: A method of a pretreatment of a sample for quantitating cholesterol characterized by, before measuring cholesterol contained in specific lipoproteins, treating the sample containing lipoproteins with an enzyme, the substrate of which is free cholesterol, optionally together with a reaction accelerator; a method for quantitating cholesterol in specific lipoproteins by using the above method; and a kit for quantitating cholesterol in specific lipoproteins to be used in the above quantification method. By using this quantification method, cholesterol in a specific fraction can be conveniently, accurately and efficiently quantitated fundamentally without resort to polyanion, etc. Thus, this method is appropriately usable in various automatic analyzers.Type: GrantFiled: July 26, 2004Date of Patent: February 26, 2008Assignee: Daiichi Pure Chemicals Co., Ltd.Inventors: Mitsuhiro Nakamura, Yuriko Taniguchi, Mitsuhisa Manabe, Mitsuaki Yamamoto
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Patent number: 7335484Abstract: Amino acid sequences and corresponding nucleic acid sequence of retinoid metabolizing protein found in human, mouse and zebrafish are described, as well as methods of using same.Type: GrantFiled: May 28, 2004Date of Patent: February 26, 2008Assignee: Queen's University at KingstonInventors: P. Martin Petkovich, Jay A. White, Barbara R. Beckett, Glenville Jones
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Patent number: 7335485Abstract: The present invention provides a method of determining the antibiotic susceptibility of a microorganism comprising the following steps. First, a culture of the microorganism whose susceptibility is to be determined is admixed with an antibiotic to which susceptibility is to be assayed, and a permeabilizing agent for the microorganism present in a non-growth-inhibiting microorganism-permeabilizing effective amount to form an assay culture. Next, the assay culture is incubated under appropriate culture conditions and for a time sufficient to determine the susceptibility of the microorganism to the antibiotic.Type: GrantFiled: December 8, 2006Date of Patent: February 26, 2008Assignee: Creighton UniversityInventors: Jennifer A. Black, Ellen S. Moland, Kenneth Thomson
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Patent number: 7335486Abstract: The invention provides methods for making peptides from a polypeptide containing at least one copy of the peptide using clostripain to excise the peptide from the polypeptide. The methods enable the use of a single, highly efficient enzymatic cleavage to produce any desired peptide sequence.Type: GrantFiled: November 24, 2004Date of Patent: February 26, 2008Assignee: Restoragen, Inc.Inventors: Fred W. Wagner, Peng Luan, Yuannan Xia, Daniel Strydom, Edwin H. Merrifield, Mary J. Bossard, Barton Holmquist, Jin Seog Seo
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Patent number: 7335487Abstract: This invention provides novel genes and polypeptides of the sweet receptor family, methods for production of the polypeptides, methods for screening compounds that specifically bind to and/or modulate the activity of these polypeptides; and antibodies specific for the polypeptides.Type: GrantFiled: September 17, 2002Date of Patent: February 26, 2008Assignees: IRM LLC, The Scripps Research InstituteInventors: Jiayu Liao, Sheng Ding, Peter G. Schultz
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Patent number: 7335488Abstract: The present invention is directed to novel spontaneously fluorescent proteins having a unique chromophore formed from the amino acid sequence FR1R2R3F where R1 is Q or S; R2 is Y, W, F or H; and R3 is G, A or S (SEQ ID NO:34). The invention also encompasses the expression of nucleic acids that encode the proteins of the invention in a wide variety of engineered host cells, and the isolation of engineered proteins. In other embodiments, the invention comprises methods of use, generally including tagging a molecule or cell with the proteins of the invention by either chemical means or recombinant techniques.Type: GrantFiled: April 2, 2003Date of Patent: February 26, 2008Assignee: The Chinese University of Hong KongInventors: David Chi-Cheong Wan, Denis Tsz-Ming Ip
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Patent number: 7335489Abstract: Human polypeptides and DNA (RNA) encoding such polypeptides and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such polypeptides for therapeutic purposes. Antagonists against such polypeptides and their use as a therapeutic are also disclosed. Also disclosed are diagnostic methods for detecting disease which utilize the sequences and polypeptides.Type: GrantFiled: June 4, 2003Date of Patent: February 26, 2008Assignee: Human Genome Sciences, Inc.Inventors: Paul A. Moore, Reiner L. Gentz, Henry Hongjun Ji, Jian Ni, Jing-Shan Hu, Craig A. Rosen
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Patent number: 7335490Abstract: Provided are uses of erythropoietin, or a derivative or functional analogue thereof, for the production of a medicament for the preventive or curative treatment of patients suffering from, or at risk of suffering from, cardiac failure.Type: GrantFiled: January 9, 2003Date of Patent: February 26, 2008Assignees: Crucell Holland B.V., Stichting Klinische Farmacologie GroningenInventors: Wiekert Hendrikus Van Gilst, Ronald Hendrik Peter Brus, Dirk Jan Van Veldhuisen, Robert Henk Henning, Rudolf Allert De Boer
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Patent number: 7335491Abstract: An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift, typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided.Type: GrantFiled: August 25, 2005Date of Patent: February 26, 2008Assignee: Wyeth Research Ireland LimitedInventors: Denis Drapeau, Yen-Tung Luan, James R. Mercer, Wenge Wang, Daniel R. Lasko
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Patent number: 7335492Abstract: The present invention relates to isolation and purification of protein in aqueous two-phase systems (ATPS). Specifically, the invention provides processes for partitioning of proteins of interest in ATPS by fusing said proteins to targeting proteins which have the ability of carrying said protein into one of the phases.Type: GrantFiled: October 19, 2005Date of Patent: February 26, 2008Assignee: Valtion Teknillinen TutkimuskeskusInventors: Merja Penttilä, Tiina Nakari-Setälä, Richard Fagerström, Klaus Selber, Maria-Regina Kula, Markus Linder, Folke Tjerneld
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Patent number: 7335493Abstract: The invention provides isolated polypeptide and nucleic acid sequences derived from Streptococcus pneumoniae that are useful in diagnosis and therapy of pathological conditions; antibodies against the polypeptides; and methods for the production of the polypeptides. The invention also provides methods for the detection, prevention and treatment of pathological conditions resulting from bacterial infection.Type: GrantFiled: December 30, 2004Date of Patent: February 26, 2008Assignee: sanofi pasteur limitedInventors: Lynn Doucette-Stamm, David Bush, Qiandong Zeng, Timothy Opperman, Chad Eric Houseweart
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Patent number: 7335494Abstract: The invention provides isolated polypeptide and nucleic acid sequences derived from Streptococcus pneumoniae that are useful in diagnosis and therapy of pathological conditions; antibodies against the polypeptides; and methods for the production of the polypeptides. The invention also provides methods for the detection, prevention and treatment of pathological conditions resulting from bacterial infection.Type: GrantFiled: December 30, 2004Date of Patent: February 26, 2008Assignee: sanofi pasteur limitedInventors: Lynn Doucette-Stamm, David Bush, Qiandong Zeng, Timothy Opperman, Chad Eric Houseweart
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Patent number: 7335495Abstract: The present invention describes the development and standardization of a facile method for the isolation and purification of cyclosporin A from a new source, viz., Fusarium nivale and evaluation of its novel antifungal activity against the food spoilage Aspargellius strains and few dermatophytes.Type: GrantFiled: March 27, 2002Date of Patent: February 26, 2008Assignee: Council of Scientific and Industrial ResearchInventors: Siddarth Bhosale, Chandrakant Govid Naik, Perunnikulath Subrayan Parameswaran
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Patent number: 7335496Abstract: A target substance is produced by culturing a bacterium which has the ability to produce the target substance in a medium to cause accumulation of said target substance in the medium and collecting the target substance from the medium, wherein the bacterium is modified so that a system for uptake of a byproduct of the target substance or a substrate for a biosynthesis system of the target substance into the bacterial cell.Type: GrantFiled: June 3, 2004Date of Patent: February 26, 2008Assignee: Ajinomoto Co., Inc.Inventors: Yoko Yamamoto, Hisao Ito
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Patent number: 7335497Abstract: A method for efficiently producing theanine is provided, wherein glutamine and ethylamine are reacted by Pseudomonas citronellosis GEA FERM BP-8353, which is newly separated/selected from natural soil, belongs to the genus, Pseudomonas, and the species citronellosis and is a theanine producing bacteria with activities of transferring the ?-glutamyl group. Theanine production increased by four times by using glutaminase derived from the bacteria in a mixture of glutamine and ethylamine at a pH in a range of 9-12 in comparison with conventional methods.Type: GrantFiled: April 22, 2003Date of Patent: February 26, 2008Assignee: Taiyokagaku Co., Ltd.Inventors: Takashi Tachiki, Yukitaka Okada, Makoto Ozeki, Tsutomu Okubo, Lekh Raj Juneja, Nagahiro Yamazaki
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Patent number: 7335498Abstract: The present invention provides a protein having saponin-decomposing activity, more specifically a protein which can decompose a glycoside having soyasapogenol B as an aglycone to produce soyasapogenol B, a polynucleotide encoding such a protein, and a method of producing soyasapogenol B on a large scale using the same. A protein according to the present invention are concerned with (a), (b) or (c), namely (a) a protein comprising an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOs: 2, 4, and 6; (b) a protein that has at least 50% homology to the protein comprising the amino acid sequence of the sequence described in (a) and having saponin-decomposing activity; or (c) a protein comprising a modified amino acid sequence of the sequence described in (a) that has one or more amino acid residues deleted, substituted, inserted, or added and having saponin-decomposing activity.Type: GrantFiled: August 16, 2006Date of Patent: February 26, 2008Assignee: Meiji Seika Kaisha, Ltd.Inventors: Manabu Watanabe, Naoki Mido, Takayoshi Tamura, Naomi Sumida, Takashi Yaguchi
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Patent number: 7335499Abstract: The invention provides tRNA synthetase polypeptides and DNA (RNA) encoding tRNA synthetase polypetides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing tRNA synthetase polypeptide for the protection against infection, particularly bacterial infections.Type: GrantFiled: January 24, 2006Date of Patent: February 26, 2008Assignee: Replidyne, Inc.Inventors: John Edward Hodgson, Elizabeth Jane Lawlor
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Patent number: 7335500Abstract: This invention contemplates improved methods of enzymatic production of carbohydrates especially fucosylated carbohydrates. Improved syntheses of glycosyl 1- or 2-phosphates using both chemical and enzymatic means are also contemplated. The phosphorylated glycosides are then used to produce sugar nucleotides that are in turn used as donor sugars for glycosylation of acceptor carbohydrates. Especially preferred herein is the use of a disclosed method for fucosylation.Type: GrantFiled: November 19, 2001Date of Patent: February 26, 2008Assignee: The Scripps Research InstituteInventors: Chi-Huey Wong, Yoshitaka Ichikawa, Gwo-Jenn Shen, Kun-Chin Liu
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Patent number: 7335501Abstract: The present invention relates to novel phytases, in particular, of fungal origin, and also to their respective methods of production. The present invention relates more particularly to novel phytases derived from fungi of the Penicillium genus, in particular of the Penicillium sp. CBS 109899 strain, and also to the polynucleotides encoding these phytases. The invention also relates to vectors containing the polynucleotides, and to transformed host organisms expressing the phytases.Type: GrantFiled: June 10, 2004Date of Patent: February 26, 2008Assignee: Adisseo France S.A.S.Inventors: Jerome Pierrard, Ralph Bohlmann, Olivier Nore, Didier Saunier, Olivier Testeniere, Fanny Moussu
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Patent number: 7335502Abstract: The present invention is to provide a gene having asymmetric hydrolase activity which is useful for synthesis of an optically active carboxylic acid, its antipode ester, and lactone, and a hydroxycarboxylic ester asymmetric hydrolase enzyme (EnHCH) derived from Enterobacter sp. DS-S-75 strain (FERM BP-5494) which is bacteria belonging to the genus Enterobacter, a EnHCH gene shown by base sequence of SEQ. ID. NO: 1, a gene encoding a protein having an amino acid sequence of SEQ. ID. NO: 2, and E. coli DH5? (pKK-EnHCH) deposited to International Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology as a deposition No. FERM BP-08466.Type: GrantFiled: July 20, 2006Date of Patent: February 26, 2008Assignee: Daiso Co., Ltd.Inventors: Atsushi Nakagawa, Toshio Suzuki, Atsuhiko Shinmyo, Ko Kato, Hideaki Idogaki
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Patent number: 7335503Abstract: The present invention relates to filamentous fungal host cells and particularly Trichoderma host cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity.Type: GrantFiled: December 4, 2006Date of Patent: February 26, 2008Assignee: Genencor International, Inc.Inventors: Toby M. Baldwin, Benjamin S. Bower, Nigel Dunn-Coleman, Suzanne E. Lantz, Michael J. Pepsin
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Patent number: 7335504Abstract: The present invention provides engineered enzymes generated from protein scaffolds combined with Specificity Determining Regions, the production thereof and the use of said engineered enzymes for research, nutritional care, personal care and industrial purposes.Type: GrantFiled: June 18, 2004Date of Patent: February 26, 2008Assignee: DIREVO Biotechnology AGInventors: Ulrich Haupts, Andre Koltermann, Andreas Scheidig, Christian Votsmeier, Ulrich Kettling
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Patent number: 7335505Abstract: The present invention provides methods of proteolytically converting a precursor protein (e.g. tau) to a product fragment (e.g., a 12 kd fragment) in a stable cell line, wherein the precursor protein is associated with a disease state in which the precursor protein aggregates pathologically (e.g. a tauopathy), and the methods comprise: (a) providing a stable cell line transfected with nucleic acid encoding: (i) a template fragment of the precursor protein such that the template fragment is constitutively expressed in the cell at a level which is not toxic to the cell; and (ii) the precursor protein, which protein is inducibly expressed in the cell in response to a stimulus, whereby interaction of the template fragment with the precursor protein causes a conformational change in the precursor protein such as to cause aggregation and proteolytic processing of the precursor protein to the product fragment.Type: GrantFiled: January 15, 2002Date of Patent: February 26, 2008Assignee: Wista Laboratories Ltd.Inventors: Claude Michel Wischik, David Horsley, Janet Elizabeth Rickard, Charles Robert Harrington
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Patent number: 7335506Abstract: A DNA encoding a variant of a protein, the protein having a loop region and six hydrophobic helices and involved in secretion of L-lysine to the outside of a cell, wherein the DNA encodes a variant of a protein not containing the loop region and facilitates secretion of L-lysine, L-arginine or both of these L-amino acids to the outside of a methanol-assimilating bacterium when the DNA is introduced into the bacterium, specifically lysE24, is introduced into a Methylobacillus bacteria to improve L-amino acid productivity, especially L-lysine and L-arginine productivities.Type: GrantFiled: November 20, 2003Date of Patent: February 26, 2008Assignee: Ajinomoto Co., Inc.Inventors: Yoshiya Gunji, Hisashi Yasueda
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Patent number: 7335507Abstract: Sperm cells are stained according to processes that involve the combining of sperm cells with a fluorescent DNA selective dye at an elevated temperature in excess of about 40° C. The methods allow for a decreased staining time. The cells may thereafter be efficiently sorted according to common separation methods, including flow cytometry.Type: GrantFiled: March 29, 2004Date of Patent: February 26, 2008Assignee: Monsanto Technology LLCInventors: Muhammad Anzar, Cindy L. Ludwig, Jeffrey A. Graham, Jeanette A. Glaenzer
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Patent number: 7335508Abstract: The present invention relates to porous freeze-dried plasma protein matrices having an open channel structure and a concentration gradient of plasma proteins crosslinked by the action of thrombin, and methods of producing said matrices. The compositions of the present invention are useful clinically, per se or as cell-bearing implants.Type: GrantFiled: July 22, 2004Date of Patent: February 26, 2008Assignee: ProChon Biotech Ltd.Inventors: Avner Yayon, Hilla Barkay, Malkit Azachi
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Patent number: 7335509Abstract: Novel stable, concentrated, biologically active and ready-to-use lipid-comprising drug delivery complexes and methods for their production are described. The biological activity of the complexes produced are comparable to the formulations prepared according to the prior art admixture method and upon purification, the complexes produced by the method of this invention are 50 to 500 fold more concentrated than the complexes formed by admixture. The method described herein provides for the large scale production of lipid-comprising drug delivery systems useful for gene therapy and other applications.Type: GrantFiled: December 15, 2004Date of Patent: February 26, 2008Assignee: University of PittsburghInventors: Leaf Huang, Xiang Gao, Frank L. Sorgi
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Patent number: 7335510Abstract: Methods and materials for modulating (e.g., increasing or decreasing) nitrogen levels in plants are disclosed. For example, nucleic acids encoding nitrogen-modulating polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Plants and plant products having increased nitrogen levels are also disclosed.Type: GrantFiled: December 2, 2005Date of Patent: February 26, 2008Assignee: Ceres, Inc.Inventors: Richard Schneeberger, Emilio Margolles-Clark, Joon-Hyun Park, Boris Jankowski, Steven Craig Bobzin
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Patent number: 7335511Abstract: Robust classification methods analyze magnetic resonance spectroscopy (MRS) data (spectra) of fine needle aspirates taken from breast tumors. The resultant data when compared with the histopathology and clinical criteria provide computerized classification-based diagnosis and prognosis with a very high degree of accuracy and reliability. Diagnostic correlation performed between the spectra and standard synoptic pathology findings contain detail regarding the pathology (malignant versus benign), vascular invasion by the primary cancer and lymph node involvement of the excised axillary lymph nodes. The classification strategy consisted of three stages: pre-processing of MR magnitude spectra to identify optimal spectral regions, cross-validated Linear Discriminant Analysis, and classification aggregation via Computerised Consensus Diagnosis. Malignant tissue was distinguished from benign lesions with an overall accuracy of 93%.Type: GrantFiled: December 15, 2004Date of Patent: February 26, 2008Assignees: University of Sydney, National Research Council of CanadaInventors: Carolyn E. Mountford, Peter Russell, Ian C. P. Smith, Rajmund L. Somorjai
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Patent number: 7335512Abstract: The invention provides methods and kits to detect liver cirrhosis in mammals. The diagnostic test is based on the profiling and identification of diagnostic carbohydrates present in a body fluid such as blood serum.Type: GrantFiled: October 18, 2004Date of Patent: February 26, 2008Assignees: Vlaams Interubiversitair Instituut voor Biotechnologie vzw, Universiteit GentInventors: Nico L. M. Callewaert, Roland H. Contreras
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Patent number: 7335513Abstract: A parallel reactor system including a reactor and vessels in the reactor for holding reaction mixtures, and a cannula for introducing fluid reaction material into the vessels. A robot system is operable to insert the cannula into cannula passages in the reactor for delivery of reaction materials, including condensed gases, to respective vessels, and to withdraw the cannula from the cannula passages after delivery. Related methods are also disclosed.Type: GrantFiled: January 28, 2002Date of Patent: February 26, 2008Assignee: Symyx Technologies, Inc.Inventors: Matthew F. Smith, John F. Varni, G. Cameron Dales, Trevor G. Frank, Rakesh Jain, Lynn Van Erden
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Patent number: 7335514Abstract: The present invention relates to an optical biosensor comprising a porous matrix. In the specific case, reference is made to anodized porous alumina, on the surface of which the biological component specific for the analyte in question is immobilized, and to an optical-signal detector connected to said matrix. The present patent further relates to a biosensor having the porous matrix and the optical detector integrated in a single structure, in particular to biosensors with porous matrix other than porous alumina, for example porous silicon.Type: GrantFiled: June 2, 2004Date of Patent: February 26, 2008Assignee: CRF Societa Consortile per AzioniInventors: Valentina Grasso, Federica Valerio, Vito Guido Lambertini, Marco Pizzi, Piero Perlo
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Patent number: 7335515Abstract: A (1?3)-?-D-glucan binding protein, a fluorescence-labeled (1?3)-?-D-glucan binding domain protein, a (1?3)-?-D-glucan measuring agent comprising the same, a method for measuring (1?3)-?-D-glucan using the same, and a (1?3)-?-D-glucan assay kit comprising the same.Type: GrantFiled: November 17, 2006Date of Patent: February 26, 2008Assignee: Seikagaku Kogyo Kabushiki KaishaInventors: Hiroshi Tamura, Masayuki Tanaka, Tatsushi Muta
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Patent number: 7335516Abstract: A method of improving specific immune responses to small immunogens, haptens, has been developed by changing the linkage between the hapten and carrier being used for immunization. Antibodies to a glycated protein have been developed, utilizing an immunogen which is composed of a glycated peptide mimic of the glycated peptide sequence which is the target epitope, wherein the peptide mimic is constructed to conformationally mimic the conformation of the peptide in the native protein, the peptide mimic contains no charged groups or other immunodominant group, and the peptide mimic is connected to a spacer sequence equivalent to a peptide spacer of between one and thirty amino acids in length, which serves to position the peptide epitope in a conformation that approximates its conformation in the native protein. In a further embodiment the peptide mimic and spacer are linked to a carrier molecule.Type: GrantFiled: August 21, 2002Date of Patent: February 26, 2008Assignee: Serex, Inc.Inventors: Judith Fitzpatrick, Regina Lenda
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Patent number: 7335517Abstract: A multichip semiconductor device is disclosed in which chips are stacked each of which comprises a semiconductor substrate formed on top with circuit components and an interlayer insulating film formed on the top of the semiconductor substrate. At least one of the chips has a connect plug of a metal formed in a through hole that passes through the semiconductor substrate and the interlayer insulating film. The chip with the connect plug is electrically connected with another chip by that connect plug.Type: GrantFiled: July 30, 2004Date of Patent: February 26, 2008Assignee: Kabushiki Kaisha ToshibaInventors: Nobuo Hayasaka, Katsuya Okumura, Keiichi Sasaki, Mie Matsuo