Abstract: A method for the purification of lecithin, comprising the steps of: a. reducing the viscosity of lecithin to a viscosity of less than about 10 Pa·s; then b. mixing the lecithin with granulated active carbon; then c. separating the lecithin from the granulated active carbon and recover purified lecithin. Lecithin substantially free of poly-aromatic hydrocarbons, and a food or feed product comprising said lecithin.

Abstract: What is proposed are specific tetrahydrofuran derivatives of the formula (I), fragrance and aroma substance mixtures comprising these tetrahydrofuran derivatives, their use in fragrance or aroma substance (mixture) and corresponding perfumed products.

Abstract: Described herein are softening compositions comprising fermented fruit solutions and softening agents, methods for making the same, and methods for using the same. The fermented fruit solutions can contain fruit, sugar and water. The softening agent can be selected from the group consisting of soy lecithin, oleic acid, glycerol, and mixtures thereof. The softening compositions can be used to soften article(s) (e.g., using as a fabric softener).

Abstract: The present invention relates to a composition and method for treating a textile under industrial and institutional fabric care conditions to impart softness with reduced yellowing. More particularly, the present invention relates to a solid fabric conditioning composition and a method for treating a textile with a solid fabric conditioning composition.

Abstract: Cleaning compositions are disclosed herein. The cleaning compositions comprises a reaction product of an alkoxylated polyol or ester thereof, and a dicarboxylic acid. Methods of cleaning hard surfaces using the cleaning compositions and formulations of the cleaning compositions are also disclosed. Methods of treating hard surfaces to improve soil resistance of a hard surface are also disclosed.

Abstract: A laundry detergent composition, comprising: by weight of the composition, from 0.001% to 3% of a nonionic anti-microbial agent, and from 0.05% to 5% of a perfume microcapsule that comprises a shell and a core of perfume oil encapsulated within the shell. The laundry detergent composition provides treated fabrics with improved freshness, particularly with both long-lasting freshness and effective malodor control against a broad spectrum of bacteria.

Abstract: The present invention relates to cleaners for hard surfaces which comprise surfactants and also phosphoric acid esters of a polyether-modified alkyl alcohol, and to the method of use thereof for cleaning hard surfaces and for generating shine on a hard surface.

Abstract: The present invention relates to cleaning composition, preferably a laundry detergent composition, comprising a cationic polymer capable of improving the overall sudsing profile of such cleaning composition.

Abstract: The need for a structuring premix, having lower viscosities, which are more suitable for use in low water liquid compositions, is met by emulsifying a non-polymeric, crystalline, hydroxyl-containing structuring agent using an alkyl sulphate.

Abstract: The present invention relates to the cleaning or laundering of fabrics and textiles whereby enzymes replace some of the surfactants, in particular in industrial applications.

Abstract: A laundry detergent is provided that effectively cleans and whitens textile substrates, but also provides decreased staining of the substrate when used during the laundering process.

Abstract: A water-soluble pouch, comprising a water-soluble film and a liquid detergent composition contained within the water-soluble film, wherein the liquid detergent composition comprises: by weight of the composition, from 0.001% to 3% of a nonionic anti-microbial agent and from 1% to 12% of water. The water-soluble pouch provides a good anti-microbial benefit, without compromising its stability.

Abstract: A device and a method for heating a fermentable starting product in order to produce a beverage, comprises a line which is arranged inside a combustion chamber and via which a part of a heat outputted from a heat source in the combustion chamber by a first heat transporting medium can be transferred to the fermentable starting product flowing in the line. A heat storage device arranged downstream of the combustion chamber is used for storing a part of the residual heat transported by means of the first heat transporting medium. The line is arranged such that the fermentable starting product flowing in the line is pre-heated upstream of the combustion chamber in a pre-heating chamber by the heat stored in the heat storage device.

Abstract: The present technology relates to novel mycotoxin binder and the use in animal feed. The present disclosure relates also to the use of enzymes for improving the mycotoxin binding ability of by-products derived from a fermentative production process and to compositions comprising enzymes capable of degrading components in the fermented mash in the fermentation process.

Abstract: The present invention provides an alcoholic drink composition having functionality to shorten drunkenness time, wherein the alcoholic drink composition is fabricated by mixing a plurality of gold flakes into an alcoholic drink with a specific mix ration. Taking sorghum liquor as an exemplary alcoholic drink composition, wherein the gold flakes are mixed into the sorghum liquor by the mix ratio ranged between 6.6 mg/600 cc and 66 mg/600 cc. Therefore, when a user drinks the sorghum liquor mixed with the gold flakes, the user would sober up after a longest drunkenness time of 59 minutes passed, without additionally taking any other anti-alcoholic products.

Abstract: A method of forming an implantable tissue engineered construct, a bioreactor for forming a tissue engineered construct, and a tissue engineered construct itself are disclosed The method includes seeding a scaffold with cells to form a tissue construct; locating the tissue construct in a space defined by a tissue construct support element; locating the tissue construct support element within a bioreactor; and operating a load applicator of the bioreactor to apply a cyclical compressive mechanical load to the tissue construct, to stimulate the deposition of tissue matrix in the tissue construct, in which the tissue construct, the tissue construct support element and the load applicator are arranged so that the load applicator can at least initially contact both the tissue construct and the tissue construct support element, so that at least part of a total load generated by the load applicator is borne by the tissue construct support element.

Abstract: Present invention relates to a modular system for fabrication of a biocolumn for generating fuel stocks. The biocolumn of independent units called modules and which function as independent units and can be assembled together to fabricate a biocolumn. These modules can be assembled together to form various zones of biocolumn. Fuel stocks can be prepared by inputting a nutrient, a renewable energy source, photon energy and a carbon source into said zones and outputting fuel stock and by products from zones. The zones are interconnected so that byproducts from each zone can be recycled as input or transformed into product.

Abstract: The disclosure provides a system and method to safely and efficiently store and transport process tubes in a carrier tray comprising prior to and during amplification of nucleotides in the process tubes. The process tube disclosed includes a securement region having an annular ledge, a neck, and a protrusion. The securement region of the process tube can secure the process tube in a port of the carrier tray, but still allows the process tube to adjust or float in order to align the process tube into a rigid heater well of a thermal cycler.

Abstract: One aspect relates to a container for inserting into a holding device, comprising: a wall that is flexible at least in some areas, which wall is squashed, at least in some areas, in a transport condition in a direction opposite to a stretching direction; a handling unit connected to the wall, which is designed to come into engagement with a complementary handling unit of a transport device; a securing unit which in a transport condition hinders stretching of the wall of the bioreactor container along the stretching direction, wherein the container can, when the securing unit is released, be extended at least in some areas along the stretching direction, as well as a transport device, a use and a method.

Abstract: An autologous cell concentrating system and method are disclosed. The system has a blood separation component, a first vessel, a second vessel, a first valve, a second valve, and a concentration and flow logic and control component. The concentration and flow logic and control component is configured to: determine a first volume of a target cell-poor fraction in the first vessel to mix with a target cell-rich fraction in the second vessel in order to form a target cell-rich concentrate having a concentration of target cells that is within a target concentration range; and control the second valve to transfer the first volume of the target cell-rich fraction from the first vessel to the second vessel to form the target cell-rich concentrate. The target concentration range is between 1.0 and 1.5×106 target cells/?L.

Abstract: The process provides a biofilm including and not limited to cellulose produced by bacteria that can be used as a bio-resin and as a surface application for myceliated and non-myceliated biomaterials. In one embodiment, the process comprises the steps of obtaining an agricultural substrate; and cohabitating a selected bacteria with a selected fungus in the agricultural substrate for a period of time to allow the bacteria to grow alongside the fungus and to excrete a biofilm from the bacteria into the substrate to provide bio-resin like strengthening compounds to the agricultural substrate.

Abstract: The invention pertains to a process for the preparation of multicellular spheroids from a suspension of single cells, wherein the cells are directly derived from a biological tissue and/or from cell-containing bodily fluid. The invention is further directed to the multicellular spheroids obtained by the process according to the invention as well as to the use of the spheroids for diagnostic, screening and therapeutic purposes.

Abstract: The present invention relates to a medium for storing, preserving, culturing, and/or differentiating stem cells. The medium employs a synthetic thermo-responsive copolymer, dispersed in an aqueous vehicle, which allows the medium to be readily “switched” between a non-gelatinous (fluid) form and a gelatinous form by simply adjusting temperature across the gelling temperature of the medium. As such, stem cells can be easily captured within a 3D gel matrix by simply mixing the stem cells with the non-gelatinous/fluid form of the medium and then gelling the medium by adjusting the temperature across the gelling temperature of the medium. The stem cells encapsulated within the gelatinous form of the medium may then be preserved for a significant time period before either using the stem cells directly within the medium (e.g. in therapy, or in culturing, differentiation, and such like), or after their extraction from the medium.

Abstract: An object of the present invention is to provide a method for introducing a target substance into undifferentiated cells, and a carrier therefor, whereby the target substance can be specifically introduced into the undifferentiated cells by contacting the undifferentiated cells with an rBC2LCN-target substance fusion product in which rBC2LCN lectin is fused to the target substance. Particularly, an rBC2LCN-toxin fusion product in which rBC2LCN lectin is fused to a toxin functioning in cells or its domain having the ability to kill cells functions as an agent for eliminating undifferentiated stem cells and can be administered into a medium after inducing the differentiation of the stem cells to reliably kill only the stem cells in an undifferentiated state.

Abstract: The present invention relates to compositions and methods for preparing cells from avian embryos. The invention also relates to cultures of such cells and the uses thereof, particularly for producing viruses such as MDV. The invention also relates to methods for the preparation of vaccines using such cells or viruses.

Abstract: The present invention relates to methods and compositions for use in generating induced hepatocytes by reprogramming non-hepatocyte cells.

Abstract: Described is an isolated somatic stem cell that is 2 to less than 6 micrometer in size and Lgr5+. Methods of preparing and using the cell are also described.

Abstract: The present invention provides adipose-derived stem cells (ADSCs), adipose-derived stem cell-enriched fractions (ADSC-EF) and adipose-derived lattices, alone and combined with the ADSCs of the invention. In one aspect, the present invention provides an ADSC substantially free of adipocytes and red blood cells and clonal populations of connective tissue stem cells. The ADSCs can be employed, alone or within biologically-compatible compositions, to generate differentiated tissues and structures, both in vivo and in vitro. Additionally, the ADSCs can be expanded and cultured to produce molecules such as hormones, and to provide conditioned culture media for supporting the growth and expansion of other cell populations. In another aspect, the present invention provides a adipose-derived lattice substantially devoid of cells, which includes extracellular matrix material from adipose tissue.

Abstract: The present invention relates to a method for producing clinically effective quantities of human adipose tissue-derived stromal cells for treating fistulas and a composition made with the same. The method of the present invention can efficiently produce clinically effective number of adipose tissue-derived stromal cells within a short period by improving conventional standard culturing methods. The adipose stem cells composition containing the adipose tissue-derived stromal cells obtained by the method of the present invention exhibit superior multipotency and immunomodulatory activity over those of a cell composition produced by conventional methods, and thus is more suitable for treating fistulas. The cell composition of the present invention has excellent clinical usages especially since immune response is suppressed in allogenic adipose-derived stem cells.

Abstract: Production of beta-cells from stem cells from pluripotent stem cells have always been significantly lacking in at least one of the following properties: 1) functional properties related to insulin-production and glucose signaling response, 2) mature phenotype such as biochemical markers or cell structures, 3) efficiency in production of differentiated cells. Described herein is multistep differentiation protocol which substantially overcomes all of the existing limitations. Pluripotent stem cells, including induced pluripotent stem cells (iPSCs), and embryonic stem cells (ESCs) can be differentiated using an embryoid body (EB) formation step, followed by B maturation via endothelial cells (EC) co-culturing and incubation with a sequential series of bone morphogenic protein (BMP)-related growth factor cocktails. The resulting cells displayed functional properties, including insulin-production and glucose signaling response, and mature phenotype of C-peptide expression.

Abstract: The following disclosure generally relates to methods of culturing podocytes in vitro. The cultures can be used for drug screening (such as medium or high throughput drug screening), and for studying molecular pathways involved in glomerular diseases. The disclosure also provides methods for analyzing the healthiness of podocytes in a cell culture. The disclosure also relates to diagnosis of kidney diseases.

Abstract: Subpopulations of spore-like cells expressing specific cell surface and gene expression markers are provided. In one embodiment, the cells express at least one cell surface or gene expression marker selected from the group consisting of Oct4, nanog, Zfp296, cripto, Gdf3, UtF1, Ecat1, Esg1, Sox2, Pax6, nestin, SCA-1, CD29, CD34, CD90, B1 integrin, cKit, SP-C, CC10, SF1, DAX1, and SCG10. Also provided are methods for purifying a subpopulation of spore-like cells of interest from a population of spore-like cells, and methods for inducing differentiation of the isolated spore-like cells into cells of endodermal, mesodermal or ectodermal origin. The spore-like cells can be used to generate cells originating from all three germ layers and can be used to treat a patient who has a deficiency of functional cells in any of a wide variety of tissues, including the retina, intestine, bladder, kidney, liver, lung, nervous system, or endocrine system.

Abstract: The present invention relates to methods for the release of baculovirus-expressed Virus-like Particles (VLP's) of non-enveloped viruses from insect cells.

Abstract: A method of non-covalently loading a plant picornavirus is described. The method includes contacting a plant picornavirus in solution with a molar excess of a cargo molecule to load the plant picornavirus with the cargo molecule, and then purifying the loaded plant picornavirus. Examples of cargo molecules include imaging agents, antitumor agents, and antiviral agents. Loaded plant picornaviruses prepared in this manner can be used to delivering cargo molecule to cells.

Abstract: The present disclosure pertains to the field peptide stapling and/or macrocyclization, where a structural motif is used to improve the properties of amino acid sequences (e.g. protease resistance, cellular penetration, biological activity). Also within the scope of the disclosure are methods for unstapling the S,S-tetrazine-containing amino acid sequence. The disclosure is also directed to methods for the reductive removal of thiocyanates from an amino acid sequence with cysteine to recycle back to the native amino acid sequence.

Abstract: This invention relates to mutant enzymes with enhanced properties and processes for oxidation of organic compound substrates using such enzymes.

Abstract: The present invention relates to T7 RNA polymerase variants with improved affinity for 2?-modified nucleotides compared to the wildtype as well as methods for their production and methods of using them. The present invention also relates to the 2?-modified RNA molecules produced according to the methods of the invention.

Abstract: Presented herein are polymerase enzymes for improved incorporation of nucleotide analogues, in particular nucleotides which are modified at the 3? sugar hydroxyl, as well as methods and kits using the same.

Abstract: Viral infection is a persistent cause of human disease. Guided nuclease systems target the genomes of viral infections, rendering the viruses incapacitated.

Abstract: Viral infections in mammals can be treated and prophylactically prevented by systemic administration of ranpirnase and three other ribonucleases that are highly homologous with it and that have activities that are highly similar to it. Experimental results against rabies, Middle East Respiratory Syndrome Coronavirus (“MERS-CoV”), influenza, Ebola virus, Chikungunya virus, Venezuelan equine encephalitis, canine parvovirus, adenovirus-2, respiratory syncytial virus, rhinovirus-14, and vaccinia are disclosed.

Abstract: The present invention relates to a method to cleave target nucleic acid sequence by the catalytic domain of a GIY-YIG homing endonucleases 1-Tevl. More precisely, the invention relates to the deciphering of new preferential 1-Tevl cleavage sites for efficient and specific cleavage activity. The invention concerns a method for the generation of TevI specific chimeric endonucleases to target nucleic acid sequence including such cleavage sites and methods of using same for gene editing.

Abstract: The disclosure provides for compositions, methods and kits, for reducing off-target effects of genome engineering.

Abstract: The disclosure provides for compositions, methods and kits, for reducing off-target effects of genome engineering.

Abstract: A variant polypeptide having alpha-amylase activity, wherein the variant has an amino acid sequence which, when aligned with the alpha-amylase comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue. Such a variant polypeptide may be used in the preparation of a baked product.

Abstract: The present disclosure relates to variant CBH II polypeptides that have improved specific activity, and compositions, e.g., cellulase compositions, comprising variant CBH II polypeptides. The variant CBH II polypeptides and related compositions can be used in variety of agricultural and industrial applications. The present disclosure further relates to nucleic acids encoding variant CBH II polypeptides and host cells that recombinantly express the variant CBH II polypeptides.

Abstract: The present disclosure relates to variant ?-glucosidase polypeptides that have enhanced thermostability, and compositions, e.g., cellulase compositions, comprising variant ?-glucosidase polypeptides. The variant ?-glucosidase polypeptides and related compositions can be used in variety of agricultural and industrial applications. The present disclosure further relates to nucleic acids encoding variant ?-glucosidase polypeptides and host cells that recombinantly express the variant ?-glucosidase polypeptides.

Abstract: Substituted dithiol pyrazine compounds useful as reducing agents in biologically relevant media having formula: where variables are defined herein and corresponding oxidized pyrazine dithianes. Reducing agents useful to reduce disulfide bonds, particularly in proteins, or to prevent the formation of disulfide bonds, particularly in proteins, and other biological molecules. Reducing agents useful to regulate protein function in proteins in which a sulfhydryl group is associated with biological activity. Reducing agents useful and suitable for application in a variety of biological applications, particularly as research and synthetic reagents. S-acylated dithiol pyrazine compounds, where R3 is an acyl group, are selectively activated as reducing agents by removal of the S-acyl groups enzymatically or chemically. Dithiol pyrazine reducing agents and corresponding S-acylated dithiol pyrazines, immobilized on surfaces or conjugated to other chemical species, are provided.

Abstract: Disclosed are methods and isolated cells useful for the improved production of function fXa derivative protein that acts as a fXa inhibitor antidote. One aspect relates to an isolated cell comprising the r-Antidote polynucleotide and Furin polynucleotide. Another aspect relates to a method for preparing the cleaved two-chain r-Antidote by expressing, in a cell, the pre-processed r-Antidote polypeptide and a Furin polypeptide.

Abstract: The current invention discloses a genetically engineered bacterium used for the treatment of breast cancer. The said bacterium is attenuated Salmonella typhimurium VNP20009 with cloned L-methioninase gene. The method for constructing this genetically engineered bacterium and the application thereof are also disclosed herein. In the current invention, our biologic drug for the treatment of breast cancer is a type of safe, non-toxic new drug with anti-tumor activity. It can highly express methioninase through recombinant DNA technology using attenuated Salmonella typhimurium VNP20009 as a carrier, which has a strong anti-tumor activity and can meet the needs. The preparation method is simple and easy to operate, showing good application prospect.

Abstract: The present invention provides a biomolecule conjugate having one or more functionalized biomolecules wherein the biomolecule is functionalized with one or more reactive sites, and at least one polymer capable of undergoing a polymer growth reaction, wherein the polymer is attached to at least one of the reactive sites of the functionalized biomolecule and wherein the polymer envelopes the functionalized biomolecule to form a reversible nanoparticle structure which protects the biomolecule by dynamically collapsing to preserve the biomolecule when an adverse environmental stimulus is present. A method of protecting a biomolecule from environmental conditions is also provided.