Abstract: There is provided an engineered host cells comprising (a) one or more mutations in one or more endogenous genes encoding a protein associated with iron metabolism; and (b) at least one gene encoding a polypeptide having xylose isomerase activity, and methods of their use thereof.

Abstract: The present invention provides, among other things, methods of purifying messenger RNA (mRNA) including the steps of subjecting an impure preparation comprising in vitro synthesized mRNA to a denaturing condition, and purifying the mRNA from the impure preparation from step (a) by tangential flow filtration, wherein the mRNA purified from step (b) is substantially free of prematurely aborted RNA sequences and/or enzyme reagents used in in vitro synthesis.

Abstract: The technology described herein is directed to methods for modulating the rate of homology-directed repair, e.g. in methods for gene modification.

Abstract: There is described a method of extracting DNA and/or RNA from a cell or capsid, the method comprising contacting the cell or capsid with a composition comprising a quaternary ammonium compound including a silicon-containing functional group. The quaternary ammonium compound may be of general formula (I) or a derivative salt thereof wherein L is a linking group; each of R1, R2, R3, R4, R5 and R6 is independently selected from H or an optionally substituted alkyl, alkenyl, aryl or alkoxy group; and n is 0 or 1. A PCR promoting agent may be provided. The method may be one of 1 detecting and/or diagnosing a disease or medical condition.

Abstract: A eukaryotic expression vector capable of displaying a multi-chain polypeptide on the surface of a host cell is provided, such that the biological activity of the multi-chain polypeptide is exhibited at the surface of the host cell. Such a vector allows for the display of complex biologically active polypeptides, e.g., biologically active multi-chain polypeptides such as immunoglobulin Fab fragments. The present invention describes and enables the successful display of a multi-chain polypeptide on the surface of a eukaryotic host cell. Preferred vectors are described for expressing the chains of a multi-chain polypeptide in a host cell separately and independently (e.g., under separate vector control elements, and/or on separate expression vectors, thus forming a matched vector set).

Abstract: The invention provides methods and compositions useful for identifying polypeptides with desired characteristics in vitro.

Abstract: The present invention is directed to small interfering RNA molecules (siRNA) targeted against an allele of interest, and methods of using these siRNA molecules.

Abstract: The present invention provides oligonucleotides that inhibit the binding of miR-27a to VE-cadherin mRNA, particularly in the form of blockmirs. The invention also provides compositions comprising such oligonucleotides and methods of use of such oligonucleotides to modulate the activity of VE-cadherin, inhibit or reduce vascular permeability, treat or prevent a vascular permeability-associated disease or condition, inhibit tumour growth, treat ischaemic injury, enhance recovery from ischaemic injury, treat surgical wounds and/or promotes post-operative recovery, and promote or induce angiogenesis.

Abstract: This invention provides a method for the in vivo delivery of oligonucleotides. The invention utilizes the presence of one or plurality of HES linked to an oligonucleotide to deliver a nucleic acid sequence of interest into the cytoplasm of cells and tissues of live organisms. The delivery vehicle is nontoxic to cells and organisms. Since delivery is sequence-independent and crosses membranes in a receptor-independent manner, the delivered oligonucleotide can target complementary sequences in the cytoplasm as well as in the nucleus of live cells. Sequences of bacterial or viral origin can also be targeted. The method can be used for delivery of genes coding for expression of specific proteins, antisense oligonucleotides, siRNAs, shRNAs, Dicer substrates, miRNAs, anti-miRNAs or any nucleic acid sequence in a living organism. The latter include mammals, plants, and microorganisms such as bacteria, protozoa, and viruses.

Abstract: Antisense molecules capable of binding to a selected target site in the human dystrophin gene to induce exon 53 skipping are described.

Abstract: The present invention relates to methods and compositions for the inhibition of gene expression. In particular, the present invention provides oligonucleotide-based therapeutics for the inhibition genes implicated in many diseases.

Abstract: The present invention relates to an aptamer or an active fragment thereof raised against the semi-conserved duffy binding ligand domain 1?, DBL1?, region of the Plasmodium falciparum erythrocyte membrane protein 1, PfEMPI, which aptamer has an effect against malaria, in particular severe cerebral malaria.

Abstract: Methods and kits for excising HIV-1 DNA in vivo are provided, which employ Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-Associated (cas) proteins. Vectors harboring nucleic acids encoding one or more guide RNA, wherein said guide RNA hybridizes with a target HIV-1 DNA are also provided.

Abstract: The present invention regards oligonucleotides for modulating the expression of a gene, in particular for modulating a gene responsible for a pathology of genetic, tumoural or viral origin. Moreover, the present invention relates to the use of said oligonucleotides, possibly chemically modified, for the treatment and/or the diagnosis of said diseases.

Abstract: The invention refers to an oligonucleotide consisting of 10 to 20 nucleotides of selected regions of the TGF-beta1, TGF-beta2 or TGF-beta3 nucleic acid sequence, which comprises modified nucleotides such as LNA, ENA, polyalkylene oxide-, 2?-fluoro, 2?-O-methoxy and/or 2?-O-methyl modified nucleotides. The invention further relates to pharmaceutical compositions comprising such oligonucleotide, wherein the composition or the oligonucleotide is used in a method for the prevention and/or treatment of glaucoma, posterior capsular opacification, dry eye, Marfan or Loeys-Dietz syndrome, riboblastoma, choroidcarcinoma, macular degeneration, such as age-related macular degeneration, diabetic macular endma, or cataract.

Abstract: The present invention relates to a composition for treatment or metastasis suppression of cancers which includes a p34 expression inhibitor or activity inhibitor as an active ingredient. According to the present invention, the p34 protein knock-down causes monoubiquitination of PTEN and accordingly nuclear localization of PTEN is induced, as a result, an Akt pathway which is related to survival, proliferation, invasive properties and metastatic properties of tumors is inhibited, and thus there is an effect of significantly reducing clonogenic potential and tumor forming potential of various cancer cells which simultaneously express PTEN and NEDD4-1. Consequently, the p34 gene expression inhibitor or p34 protein activity inhibitor according to the present invention can be effectively used as a treatment agent or a metastasis suppression agent for cancers.

Abstract: Various methods and compositions relating to vascular endothelial growth factor receptor 2 (VEGFR2) are provided.

Abstract: Long interfering nucleic acid (iNA) duplexes, which are at least 30 nucleotides in length, which have at least one nick or nucleotide gap in the antisense or the sense strands or in both the sense and antisense strands. These long iNA duplexes do not induce an interferon response when transfected into mammalian cells. The antisense strands can target two separate mRNAs or two segments of one mRNA.

Abstract: The disclosure relates to biological methods of making a hydrocarbon feedstock wherein one-carbon substrates are converted into useful chemicals and fuels. Particularly, genetically engineered bacteria are used to make C4-C10 fatty acids or derivatives from one-carbon substrates such as methanol and carbon dioxide.

Abstract: Provided herein are non-naturally occurring microbial organisms having a FaldFP, a FAP and/or metabolic modifications which can further include a MMP, a MOP, a hydrogenase and/or a CODH. These microbial organisms can further include a butadiene, 13BDO, CrotOH, MVC or 3-buten-1-ol pathway. Additionally provided are methods of using such microbial organisms to produce butadiene, 13BDO, CrotOH, MVC or 3-buten-1-ol.

Abstract: Provided is an optically controlled gene expression system of prokaryotic bacterium, comprising: a) a photosensitive recombinant transcription factor encoding gene, the photosensitive recombinant transcription factor is one fusion protein comprising a first polypeptide as the DNA bonding domain and a second polypeptide as the photosensitive domain; b) a target transcription unit comprising promoter or promoter-reaction element or reaction element-promoter containing at least one reaction element recognized/bound by the first polypeptide and the nucleic acid sequence to be transcribed. Also provided is a prokaryotic expression vector comprising said optically controlled gene expression system, and a method for regulating gene expression in a prokaryotic host cell by using the optically controlled gene expression system. Also provided is a reagent kit containing different components of the optically controlled gene expression system.

Abstract: Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of modulating transcription or transcription or transcriptional control using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.

Abstract: Provided are Thraustochytrid and Thraustochytrium and relevant methods and reagents, including engineered regulatory sequences and genes from and/or operative in Thraustochytrid or Thraustochytrium, selectable markers useful for engineering microorganisms such as Thraustochytrids, means for mutagenizing microorganisms, strains produced by mutagenesis, and methods and compositions related to production of particular compounds in microorganisms.

Abstract: Certain embodiments of the invention provide a method comprising: a) transforming cells from a selected plant with a vector comprising a gene encoding a heterologous target protein, to obtain transgenic primary plant cells, wherein the selected plant is from a species that produces activators or inhibitors of the heterologous target protein; b) mutagenizing an explant obtained from the transgenic primary plant cells to form mutagenized transgenic cells; and c) exposing the mutagenized transgenic cells to a compound, wherein mutagenized transgenic cells that overproduce one or more activators or inhibitors of the heterologous target protein, as compared to a non-mutant transgenic plant cell from the same species, survive; and wherein mutagenized transgenic cells that do not overproduce one or more activators or inhibitors of the heterologous target protein, as compared to a non-mutant transgenic plant cell from the same species, die.

Abstract: Compositions and methods for the expression of one or more coding sequences are provided which use an internal ribosome entry site (IRES) from Triticum mosaic virus (TriMV) to facilitate translation and expression of a polypeptide from an mRNA strand.

Abstract: The present invention relates to resveratrol-enriched transgenic rice for biosynthesizing resveratrol at high concentration, in which resveratrol synthase genes are expressibly inserted into the 12th chromosome of natural rice, and seed of rice produced therefrom. Further, the present invention relates to a health functional food composition, an animal feed composition, and a pharmaceutical composition for preventing and improving a metabolic disease, including seed of rice produced from the resveratrol-enriched transgenic rice for biosynthesizing resveratrol, in which resveratrol synthase genes are expressibly inserted into the 12th chromosome of natural rice. The seed of rice produced from resveratrol-enriched transgenic rice into which resveratrol synthase genes are inserted, containing high a concentration of resveratrol of the present invention exhibits remarkably superior effects compared to when the same amount of resveratrol is simply ingested.

Abstract: Methods of genetically modifying soybean plants to alter the fatty acid properties of the oil are described.

Abstract: The present invention relates to methods and means to increase seed weight in Brassica. More specifically, the invention relates to mutant DA1 genes in Brassica plants and the use thereof to seed weight.

Abstract: Isolated polynucleotides and polypeptides and recombinant DNA constructs particularly useful for altering agronomic characteristics of plants under nitrogen limiting conditions are described. Compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs also are described. The recombinant DNA construct may comprise a polynucleotide operably linked to a heterologous promoter functional in a plant, wherein said polynucleotide encodes a PH11 or a NUCPU29 polypeptide.

Abstract: The present invention relates to a method of increasing resistance against fungal pathogens of the order Pucciniales, preferably the family Phacopsoraceae, in plants and/or plant cells. This is achieved by increasing the expression of an HCP5 protein or fragment thereof in a plant, plant part and/or plant cell in comparison to wild type plants, wild type plant parts and/or wild type plant cells. Furthermore, the invention relates to transgenic plants, plant parts, and/or plant cells having an increased resistance against fungal pathogens, in particular, pathogens of the order Pucciniales, preferably the family Phacopsoraceae, and to recombinant expression vectors comprising a sequence that is identical or homologous to a sequence encoding an HCP5 protein.

Abstract: The present invention presents methods to alter the genetic composition of crop plants susceptible to nematode infection to improve tolerance to the same. Methods and compositions for modulating key pathways involved in the syncytial event of nematode infection and for preventing the cascade of differential gene expression caused by the same as disclosed. Applicants have found that the microRNA miR396 acts as a master switch of syncytial gene expression changes in plants after infection, and further that miR396 and certain growth regulating transcription factors (GRF) are connected through feedback interaction in syncytium initiation and maintenance.

Abstract: Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest, as probes for the isolation of other homologous (or partially homologous) genes. The insecticidal proteins find use in controlling, inhibiting growth or killing lepidopteran, coleopteran, dipteran, fungal, hemipteran, and nematode pest populations and for producing compositions with insecticidal activity.

Abstract: A fusion polynucleotide including a human CMV promoter and an intron, a recombinant vector including the fusion polynucleotide and a gene encoding a polypeptide of interest, a recombinant cell comprising the recombinant vector, and a method of producing a polypeptide of interest using the recombinant vector or recombinant cell.

Abstract: This invention relates to a DNA construct that is capable of expressing a desired transgene in a trackable manner. The construct comprises in 5? to 3? downstream direction: a promoter; a fluorescent reporter gene positioned within an intron defined by a 5?-donor splice site comprising a splice donor sequence and a 3?-acceptor splice site comprising a splice acceptor sequence; a desired gene and a transcription terminator. A method of producing a transgenic host cell having a desired product is also disclosed.

Abstract: The invention relates to a recombinant measles virus expressing a heterologous amino acid sequence derived from an antigen of a determined RNA virus, said recombinant measles virus being capable of eliciting a humoral and/or cellular immune response against measles virus or against said RNA virus or against both measles virus and against said RNA virus. It also relates to the use of said recombinant measles virus for the preparation of immunogenic composition.

Abstract: The present invention relates to new insertion sites useful for the integration of exogenous sequences into an intergenic region (IGR) of a vaccinia virus genome, where the IGR is located between or is flanked by two adjacent open reading frames (ORFs) of the vaccinia virus genome, and where the ORFs correspond to conserved genes, and to related plasmid vectors useful to insert exogenous DNA into the genome of a vaccinia virus, and further to recombinant vaccinia viruses comprising an exogenous sequence inserted into said new insertion site as a medicine or vaccine.

Abstract: This invention discloses reagents and methods for increasing specificity and efficiency of RNA-guided genome editing.

Abstract: Methods for producing an isoprenoid are provided. A plurality of bacterial or fungal host cells is obtained. These cells comprise a heterologous nucleic acid encoding one or more enzymes of a mevalonate pathway for making isopentenyl pyrophosphate. Expression of the one or more enzymes is under control one or more heterologous transcriptional regulator. The mevalonate pathway comprises (i) an enzyme that condenses acetoacetyl-CoA with acetyl-CoA to form HMG-CoA, (ii) an enzyme that converts HMG-CoA to mevalonate, (iii) an enzyme that phosphorylates mevalonate to mevalonate 5-phosphate, (iv) an enzyme that converts mevalonate 5-phosphate to mevalonate 5-pyrophosphate, and (v) an enzyme that converts mevalonate 5-pyrophosphate to isopentenyl pyrophosphate. The host cells are cultured in a medium under conditions that are suboptimal as compared to conditions for the maximum growth rate. Temperature is maintained at a level below that which would provide for a maximum specific growth rate for the host cells.

Abstract: The present disclosure identifies pathways and mechanisms to confer production of carbon-based products of interest such as ethanol, ethylene, chemicals, polymers, n-alkanes, isoprenoids, pharmaceutical products or intermediates thereof in photoautotrophic organisms such that these organisms efficiently convert carbon dioxide and light into carbon-based products of interest, and in particular the use of such organisms for the commercial production of ethanol, ethylene, chemicals, polymers, n-alkanes, isoprenoids, pharmaceutical products or intermediates thereof.

Abstract: A process for fermenting syngas and a fermentation medium provides high ethanol productivity while removing medium components that were previously thought to be essential. The process is effective for providing a specific STY of at least about 1 g ethanol/(L-day-gram cells). In this aspect, the fermentation medium has a weight ratio of NH4+ to B of about 625:1 or more, or a weight ratio of NH4+ to Mn of about 4050:1 or more, or a weight ratio of NH/ to Mo of about 2500:I or more, or a ratio of NH4+ to Cu of about 4050:I or more; or the fermentation medium has a weight ratio of P to B of about 30:1 or more, or a weight ratio of P to Mn of about 190:1 or more, or a weight ratio of P to Mo of about 120:1 or more, or a weight ratio of Mn to Cu of about 190:1 or more; or the fermentation medium has a weight ratio of K to B of about 35:1 or more, or a weight ratio of K to Mn of about 245:1 or more, or a weight ratio of K to Mo of about 150:1 or more, or a weight ratio of K to Cu of about 245:1 or more.

Abstract: The invention provides genetically engineered microorganisms with altered carbon monoxide dehydrogenase (CODH) activity and methods related thereto. In particular, the invention provides a genetically engineered carboxydotrophic acetogenic bacterium having decreased or eliminated activity of CODH1 and/or CODH2. In certain embodiments, the bacterium may also have increased activity of CODH/ACS. The invention further provides a method for producing a product by culturing the bacterium in the presence of a gaseous substrate comprising one or more of carbon monoxide, carbon dioxide, and hydrogen.

Abstract: A process for the preparation of succinic acid comprising the steps of: a) providing an aqueous medium comprising magnesium succinate by fermentation of a carbohydrate source, in the presence of a magnesium base; b) processing the aqueous medium wherein the magnesium succinate is treated with a monovalent base, prior to or after a crystallisation step, to provide a magnesium base and an aqueous solution comprising a monovalent succinate salt; c) adjusting the concentration of the monovalent succinate salt to between 10 and 35 wt. %; d) subjecting the aqueous solution to water-splitting electrodialysis, to produce a first solution comprising monovalent base and a second solution comprising succinic acid and monovalent succinate salt, the electrodialysis causing conversion of 40 to 95 mole %; e) separating the second solution into succinic acid and the monovalent succinate salt by crystallisation; f) recycling the monovalent succinate salt solution of step e) to step d).

Abstract: The present invention concerns a new method for the production of 1,3-propanediol comprising culturing a microorganism on a culture medium with high glycerine content. The invention also concerns a new microorganism, or strain of microorganism, adapted for the production of 1,3-propanediol from medium comprising high glycerine content. The invention also concerns an “adapted microorganism” which glycerol metabolism is directed to 1,3-propanediol production, and which is allowed to grow in the presence of a high concentration of industrial glycerine. The invention also concerns a biosourced 1,3-propanediol obtained by the process thereof. Finally the invention concerns the use of the above described biosourced 1,3-propanediol as extender chain in thermoplastic polyurethane, as monomers in polytrimethylene terephtalate and as a component in cosmetics formulations.

Abstract: Engineered organisms, cell-free enzyme mixtures, and methods are provided for converting both enantiomers of 1,2-propanediol to propionaldehyde. Engineered organisms are provided that convert both enantiomers of 1,2-propanediol to propionaldehyde but do not convert glycerol to 3-hydroxypropionaldehyde and/or do not convert propanal to propanol. The engineered organisms and cell-free enzyme mixtures can contain a diol dehydratase enzyme similar in sequence identity to Roseburia inulinivorans diol dehydratase. The engineered organisms and cell-free enzyme mixtures can contain a diol dehydratase activating enzyme similar in sequence identity to Roseburia inulinivorans diol dehydratase activating enzyme. Methods of converting both enantiomers of 1-2-propanediol to propanol can include culturing a microorganism provided herein under conditions and for a period of time sufficient to convert the 1,2-propanediol to propanol. The conditions can include a substantially anaerobic culture medium.

Abstract: An object of the present invention is to provide a method for producing poly(3-hydroxybutyrate-co-3-hydroxyhexanoate (P(3HB-co-3HHx)) having a high 3-hydroxyhexanoate fraction using a vegetable oil as a basic raw material. According to the present invention, a method is provided for producing P(3HB-co-3HHx) having a high 3-HHx fraction using a vegetable oil as a basic raw material by disrupting and so forth at least one gene encoding 2-enoyl-CoA hydratase or at least one gene encoding 3-hydroxyacyl-CoA dehydrogenase on a chromosome of a recombinant Cupriavidus necator strain imparted with the ability to produce P(3HB-co-3HHx).

Abstract: The present disclosure relates to bioengineering approaches for producing biofuel and, in particular, to the use of a C1 metabolizing microorganism reactor system for converting C1 substrates, such as methane or methanol, into biomass and subsequently into biofuels, bioplastics, or the like.

Abstract: The present invention provides methods for catalyzing a nitrene insertion into a C—H bond to produce a product having a new C—N bond, comprising providing a C—H containing substrate, a nitrene precursor and an engineered heme enzyme; and allowing the reaction to proceed for a time sufficient to form a regioselective product having a new C—N bond.

Abstract: The present invention relates to microorganisms of corynebacterium which can utilize xylose and to a method for producing L-lysine using same. More particularly, the present invention relates to microorganisms of corynebacterium which are modified, in which genes encoding xylose isomerase and xylulokinase which are xylose synthases are introduced to express the xylose synthase. The present invention also relates to a method for producing L-lysine, comprising a step of culturing the modified microorganisms of corynebacterium using xylose as a carbon source, and recovering L-lysine from the culture.

Abstract: A novel process is provided for the efficient preparation of an asymmetric compound of structural formula I: employing dynamic kinetic resolution (DKR). The DKR process involves an enzymatic enantioselective amination reaction catalyzed by transaminases. The process can be used to manufacture key intermediates in the preparation of poly (ADP-ribose) polymerase (PARP) inhibitors which may be useful for the treatment of cancer.

Abstract: A fungal alpha-amylase is provided from Aspergillus fumigatus (AfAmy1). AfAmy1 has an optimal pH of 3.5 and is operable at 30-75 degrees C., allowing the enzyme to be used in combination with a glucoamylase and a pullulanase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AfAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an alpha-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.