Abstract: Presently disclosed is a lighting system and methods of using the lighting system for in vitro potency assay for photofrin. The lighting system includes a lamp housing, a first lens, an infrared absorbing filter, an optical filter, and a second lens. The lamp housing includes a lamp and a light-port. In operation, broad spectrum light from the lamp exits the lamp housing by passing through the light-port. The first lens then collimates the broad spectrum light that exits the lamp housing through the light-port. The infrared absorbing filter then passes a first portion of the collimated broad spectrum light to the optical filter and absorbs infrared light of the broad spectrum light. The optical filter then passes a second portion of the collimated broad spectrum light to the second lens. The second lens then disperses the second portion of the collimated light to provide uniform irradiation of a cell culture plate. A method of using the lighting system for studying a photosensitizer is also disclosed.

Abstract: The present invention relates to reagents for use in deactivating nucleic acids and methods of making and using the same.

Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components.

Abstract: Compositions and methods are described for standardizing the DNA amplification efficiencies of a highly heterogeneous set of oligonucleotide primers as may typically be used to amplify a heterogeneous set of DNA templates that contains rearranged lymphoid cell DNA encoding T cell receptors (TCR) or immunoglobulins (IG). The presently disclosed embodiments are useful to overcome undesirable bias in the utilization of a subset of amplification primers, which leads to imprecision in multiplexed high throughput sequencing of amplification products to quantify unique TCR or Ig encoding genomes in a sample. Provided is a composition comprising a diverse plurality of template oligonucleotides in substantially equimolar amounts, for use as a calibration standard for amplification primer sets. Also provided are methods for identifying and correcting biased primer efficiency during amplification.

Abstract: Methods, compositions and articles of manufacture for assaying a sample for a target polynucleotide are provided. A sample suspected of containing the target polynucleotide is contacted with a polycationic multichromophore and a sensor PNA complementary to the target polynucleotide. The sensor PNA comprises a signaling chromophore to absorb energy from the excited multichromophore and emit light in the presence of the target polynucleotide. The methods can be used in multiplex form. Kits comprising reagents for performing such methods are also provided.

Abstract: Disclosed herein are methods for the automated reconstruction of a genotype of a gene, fragment, or genomic region using exhaustive enumeration. The methods can be used to reconstruct the genotype of any GC-rich sequence, such as the CGG repeat region in the 5? UTR of FMR1 or the CCG repeat region in the 5? UTR of FMR2. Also disclosed is an apparatus for use in conducting automated genotype reconstruction, as well as methods of diagnosis and treatment using exhaustive enumeration methods to reconstruct and identify genotypes associated with a disease or disorder.

Abstract: A technique is provided for forming a nanodevice for sequencing. A bottom metal contact is disposed at a location in an insulator that is on a substrate. A nonconducting material is disposed on top of the bottom metal contact and the insulator. A carbon nanotube is disposed on top of the nonconducting material. Top metal contacts are disposed on top of the carbon nanotube at the location of the bottom metal contact, where the top metal contacts are formed at opposing ends of the carbon nanotube at the location. The carbon nanotube is suspended over the bottom metal contact at the location, by etching away the nonconducting material under the carbon nanotube to expose the bottom metal contact as a bottom of a trench, while leaving the nonconducting material immediately under the top metal contacts as walls of the trench.

Abstract: An isolated nucleic acid molecule comprising a polymorphic site selected from the group consisting of positions 164, 269, 284, 407 and 989 of SEQ ID NO: 1, an array or a kit comprising the same. Also provided are a method for detecting single nucleotide polymorphism (SNP) in bovine proteinase inhibitor (PI) gene, a method for haplotyping a bovine cell, a method for progeny testing of cattle based on said haplotyping, a method for selectively breeding of cattle based on haplotyping a parent animal. The present invention further provides a method for testing a dairy cattle for its milk production trait, comprising haplotyping its cells, wherein a cattle having haplotypes 1, 3, 4 or 5 indicates that the cattle has desirable milk production trait. Haplotype 1 indicates that the cattle has the most desirable milk production trait.

Abstract: The present invention relates to compositions and methods for detection and quantification of individual target molecules in biomolecular samples. In particular, the invention relates to coded, labeled probes that are capable of binding to and identifying target molecules based on the probes' label codes. Methods of making and using such probes are also provided. The probes can be used in diagnostic, prognostic, quality control and screening applications.

Abstract: Methods are provided for the isolation of biological macromolecules including peptides, proteins, and nucleic acids from mature plant fibers such as cotton fibers and from textiles. The biological macromolecules can be used to characterize the plant fiber (source, origin, genomic composition of producing plant etc.).

Abstract: The present invention is based on the discovery of genetic polymorphisms that are associated with psoriasis and related pathologies. In particular, the present invention relates to nucleic acid molecules containing the polymorphisms, including groups of nucleic acid molecules that may be used as a signature marker set, such as a haplotype, a diplotype, variant proteins encoded by such nucleic acid molecules, reagents for detecting the polymorphic nucleic acid molecules and proteins, and methods of using the nucleic acid and proteins as well as methods of using reagents for their detection.

Abstract: The present invention concerns a method for the detection or monitoring of cancer using a biological sample selected from blood, plasma, serum, saliva, urine from an individual, said method comprising: (a) obtaining DNA from the said biological sample; (b) digesting the DNA sample with one or more methylation-sensitive restriction enzymes; (c) quantifying or detecting a DNA sequence of interest after step (b), wherein the target sequence of interest contains at least two methylation-sensitive restriction enzyme recognition sites; and (d) comparing the level of the DNA sequence from the individual to a normal standard, to detect, prognosticate or monitor cancer.

Abstract: Methods of tests that assess the expression of DPC4 (SMAD4) to identify subjects with pancreatic cancer that are likely or unlikely to respond to treatment with BTK inhibitors; methods of treating subjects based on identification of the subjects as likely to respond to treatment with BTK inhibitors; therapeutic targets for cancers, particularly cancers with inactivated DPC4 gene or protein; methods of screening of new therapeutic agents using the target; pharmaceutical composition comprising BTK inhibitors, such as PCI-32765 or derivatives thereof, for cancer treatment; and kits that facilitate the performance of the methods are disclosed.

Abstract: The invention provides methods of monitoring differential gene expression of biomarkers to determine patient sensitivity to Human Double Minute inhibitors (MDM2i), methods of determining the sensitivity of a cell to an MDM2i by measuring biomarkers and methods of screening for candidate MDM2i.

Abstract: The present invention relates to methods and kits for identifying, diagnosing, prognosing, and monitoring cervical cancer. These methods include determining the methylation status or the expression levels of particular genes, or a combination thereof.

Abstract: Mutations in calreticulin are discovered to be linked to myeloid malignancies. Disclosed are genomic sequences, cDNA sequences, mRNA sequences and protein sequences of mutant calreticulin that are linked to myeloid malignancies. Also disclosed are methods for diagnosing myeloid malignancy comprising determining the presence of a mutant allele of the calreticulin gene. Also disclosed are related compositions, kits and methods, including the medical use of inhibitors of mutant calreticulin.

Abstract: A process for granulation of hot liquid slag wherein the hot liquid slag is mixed with solid metallic particles so as to form a solidified, vitrified slag cake mixed with said metallic particles and said slag cake is discharged in a water bath.

Abstract: The present invention relates to a procedure for preparation by wet reduction method of nanometric particles of metallic silver, with diameter in the range of 1 to 100 nm and an average diameter of 20 to 40 nm, with monodispersion characteristics, stability greater than 12 months and in a wide range of concentrations. The process comprises 4 steps: a) preparation of the reducing agent solution, taken from the group of tannins and preferably being tannic acid; b) preparation of a solution of a soluble silver salt; c) reaction and, d) solid-liquid separation; the particle size is determined by the nature of the reducing agent and by the pH control of the currents. The final step is designed for separating and concentrating the material after which the user can prepare the product for integration thereof in the desired medium.

Abstract: The present invention describes an effective grain refining practice for aluminum foundry alloys. The method described herein relies on the control of the Titanium level of the alloy to be grain refined and the addition of boron once it is melted. Boron addition can be made via Al—B master alloys as well as with boron compounds such as KBF4 salt. The boron added into the melt dissolves first and then forms the AlB2 particles that act as potent substrates for the nucleation of aluminum once solidification process starts. The Ti concentration of the alloy must be controlled below 100 ppm for this method to offer effective grain refinement. The boron becomes ineffective when the Ti concentration in the alloy is higher.

Abstract: The present invention provides a structural material having enhanced ductility characteristics at high temperatures and enhanced strength characteristics. The present invention provides an Ni3(Si, Ti)-based intermetallic compound characterized by containing from 25 to 500 ppm by weight of B with respect to a weight of an intermetallic compound having a composition of 100% by atom in total consisting of Ni as a main component, from 7.5 to 12.5% by atom of Si, from 4.5 to 11.5% by atom of Ti and from 0.5 to 5.0% by atom of W.

Abstract: The invention relates to a method for manufacturing an aluminum/metal assembly consisting of a metal layer made of a first metal on the surface of a metal object made of a second metal, the first or second metal being aluminum, wherein said method includes the following steps: placing a metal foam having an open porosity on a surface of the metal object, and applying mechanical stress to the metal foam in such a manner that said foam becomes embedded in the surface of the object.

Abstract: An example of the coated tool disclosed herein includes a substrate, a metal layer established on the substrate, a continuous metal carbide layer established on the metal layer, and a smooth, continuous, terminated diamond like carbon (DLC) layer established on the metal carbide layer. The DLC layer is to prevent metal, from a workpiece upon which the tool is to act, from adhering to the tool.

Abstract: A system includes a computational system to receive a design of an integrated computational element (ICE) including specification of a substrate and a plurality of layers, their respective target thicknesses and complex indices, such that a notional ICE fabricated based on the ICE design is related to a characteristic of a sample. Additionally, the system includes a deposition chamber including a deposition source to provide a deposition plume having a plume spatial profile, and a support to support a plurality of instances of the substrate during fabrication of a plurality of instances of the ICE. The support is spaced apart from the deposition source and has a shape that corresponds to the plume spatial profile, such that when the supported instances of the substrate are distributed over the support, thicknesses of instances of each of the deposited layers are substantially uniform across the plurality of instances of the ICE.

Abstract: Provided is a tantalum coil for sputtering disposed between a substrate and a sputtering target, wherein the tantalum coil has irregularities so that the surface roughness Rz of the tantalum coil is 150 ?m or more and the number of threads is 15 to 30 TPI (Threads per inch) in a transverse direction and 10 to 30 TPI in a vertical direction. An object of the present invention is to take measures to prevent the sputtered grains accumulated on the surface of a tantalum coil from flaking so as to prevent the generation of particles and arcing that is caused by the flaking of the sputtered grains accumulated on the surface of the coil disposed between a substrate and a sputtering target, and the adhesion of the scattered flakes onto the substrate surface; and thereby to provide a technology of improving the quality and productivity of electronic components and stably providing semiconductor elements and devices.

Abstract: A thin layer of a silicon-carbon-containing film is deposited on a substrate by generating hydrogen radicals from hydrogen gas supplied to a radicals generation chamber, supplying the hydrogen radicals to a substrate processing chamber separate from the substrate processing chamber via a multiport gas distributor, and reacting the hydrogen radicals therein with an organosilicon reactant introduced into the substrate processing chamber concurrently. The hydrogen radicals are allowed to relax into a ground state in a radicals relaxation zone within the substrate processing chamber before reacting with the organosilicon reactant.

Abstract: A coated cutting insert and a method for making the same. The coated cutting insert has a substrate with a substrate surface. There is a backing coating scheme on the substrate surface, and a TiAl2O3 coating layer wherein the TiAl2O3 coating layer is deposited using chemical vapor deposition from a gaseous composition including AlCl3, H2, TiCl4, CO2 and HCl.

Abstract: In an apparatus for treating a gas stream, a plasma generator comprises an electrode for energizing a source gas to generate a plasma flare by application of a high voltage. An inlet allows the gas stream into the apparatus and directs it into the generated plasma. A scraper is fitted for reciprocating movement from a first position to a second position for scraping a surface to remove solid deposits accumulated on the surface.

Abstract: A method for manufacturing a silicon carbide thin film comprises steps of: (a) utilizing a mechanical pump to remove gases in a chamber such that the pressure in the chamber is reduced to a base pressure; (b) utilizing a microwave generator to generate microwaves at 1200 W to 1400 W so as to form microwave plasma inside the chamber; and (c) introducing into the chamber a silicon-based compound containing chlorine atoms that serve as a precursor, during the time that the temperature of a substrate disposed in the chamber is stable at 400° C. to 500° C., in which the temperature of the substrate is risen by the microwave plasma without heating the substrate additionally, so as to form a film of cubic silicon carbide on the substrate. In the present invention, the SiC thin film has good crystallinity and is manufactured by using MPECVD in a low temperature process.

Abstract: A deposition apparatus according to an exemplary embodiment of the present invention includes a plurality of reaction spaces, a plurality of plasma electrodes respectively disposed in the reaction spaces, a first plasma processor connected to at least two plasma electrodes, and a first plasma power source connected to the first plasma processor. The first plasma processor may include a plasma distributor or a plasma splitter.

Abstract: A processing chamber and a method for centering a substrate therein is provided. In one embodiment, the processing chamber includes a chamber body, a support structure, lift pins, alignment pins and restriction assemblies. The support structure has an upper surface and is disposed in the chamber body. The lift pins, alignment pins and restriction assemblies are disposed on the upper surface. The lift pins are applied for bearing the substrate. Each of the alignment pins has a conical contact surface which can be extended from the upper surface to contact the edges of the substrate to provide lateral forces to center the substrate on the lift pins. Additionally, when the alignment pins and the lift pins are retracted, the substrate is lowered and placed between the restriction assemblies disposed on the upper surface to prevent the substrate from inadvertently moving laterally from the centered position.

Abstract: The method and device are used to plasma-treat workpieces. The workpiece is inserted into a chamber of a treatment station that can be at least partially evacuated. The plasma chamber is bounded by a chamber bottom, a chamber cover, and a lateral chamber wall. The method process is optically monitored at least at times. In the optical monitoring, spectral lines of the radiation of the plasma above 500 nanometers are evaluated. Preferably, the evaluation is performed for frequencies above 700 nanometers.

Abstract: Silica nanopowders with biocidal properties, especially for polymer composites, are produced by sol-gel method. The silica sol is produced from the aqueous mixture containing tetraalkoxysilane, in which alkoxy group contains from C1 to C4 carbon atoms, an alcohol or the mixture of aliphatic alcohols from C1 to C4, in the mole ratio of 1:5 to 1:35, in the presence of ammonium compound, used in an amount of from 0.001 to 0.05 mol per 1 mol of tetraalkoxysilane, with introducing, after thorough mixing of components, the silver salt in the form of aqueous solution in an amount from 0.02 to 1 mol per 1 mol of tetraalkoxysilane, and subsequently the aqueous solution of alkali metal hydroxide in an amount from 0.02 to 1 mol of hydroxide per 1 mol of tetraalkoxysilane.

Abstract: A method manufactures a fuel delivery pipe including a crude metal made of forged iron, a nickel-phosphorus plating layer formed on an inner surface of the crude metal, and a nonmetal paint film formed on an outer surface of the crude metal. The method includes coating the outer surface of the crude metal with paint to form the paint film, machining the crude metal with the paint film formed thereon to form a machined surface inside the crude metal, and electroless plating the machined crude metal in nickel-phosphorus plating solution to form the nickel-phosphorus plating layer on the machined surface.

Abstract: The present invention relates to the application of polymeric resins and TiO2 doped with polymeric resins as coatings for external metal surfaces for industrial use, by selecting the following metals: stainless steel, carbon steel and copper. Focuses specifically on the synthesis of a polymeric resin waterborne corrosion consisting of nano-structured polymer particles formed with two or more acrylic monomers, vinyl or styrenic a functionalizing agent and a crosslinking agent from 1.0 to 20% w each. Doping addition is made of the same polymeric resins, which are incorporated in nanotubes of titanium dioxide in concentrations ranging from 50 to 10,000 ppm. The polymeric resin is water based corrosion synthesized by emulsion polymerization techniques and is during the synthesis process is introduced nanotube loading of titanium dioxide, which allowing the dispersion in the polymer matrix. Polymer dispersions obtained in this way are used as anti-corrosion coatings 100% water based.

Abstract: A photoelectrochemical method on metal preservation is disclosed that includes applying a coating on metal surface and curing the coating. The coating includes: A) the film-forming component; and B) the surface modified nanometer titanium dioxide, which consists of nanometer titanium dioxide particle as substrate and the nonionic surfactant covered on the substrate surface. The diameter of the nanometer titanium dioxide substrate is 1-100 nm. The hydrophile-lipophile balance value of the nonionic surfactant is 1-10 and is contained of 1-20 wt % based on the weight of nanometer titanium dioxide substrate. The content of the surface modified nanometer titanium dioxide is 0.1-10 wt % based on the total weight of the coating. The photoelectrochemical method on metal preservation through this disclosure is low cost and high efficient and obviously reduce the corrosion speed.

Abstract: A method for operating an installation for producing hydrogen including at least one high temperature electrolyzer for producing hydrogen, the electrolyzer including a plurality of elementary cells, each including a cathode and an anode separated by an electrolyte, the elementary cells being separated by an interconnection plate, each interconnection plate defining with an adjacent cathode a cathode compartment and with an adjacent anode an anode compartment, the cathodes being made from a cermet containing nickel, the method including: a) raising a temperature of the cells up to an operating temperature of the electrolyzer comprised between 650° C. and 950° C.; b) applying a potential difference on terminals of the elementary cells at least equal to 0.62 V.

Abstract: A method for extraction of target components from raw liquid streams includes steps of providing at least one electrochemical cell arranged to support redox reactions resulting in electrochemical change of oxidation states and concentration of at least one ionized target component, and to control at least one pH value of at least one electrolyte in the at least one electrochemical cell; introducing a raw liquid stream comprising a combination of constituent ionic species into the at least one electrochemical cell; operating the at least one electrochemical cell to change concentrations of at least two oxidation states of at least one targeted ionic species from the constituent ionic species; operating the at least one electrochemical cell to maintain a predetermined range of pH of the at least one electrolyte and to eliminate at least one target component pertinent to the at least one oxidation state of the targeted ionic species.

Abstract: The instant disclosure provides an electrolysis cell, which includes: an anode; a cathode block; and a current collector bar configured to be at least partially disposed adjacent to and in electrical communication with the cathode block, wherein the bar comprises: at least one sidewall; an inner void enclosed by the sidewall; and an expandable material retained in the inner void via the at least one sidewall, wherein the expandable material is configured to exert pressure on the wall of the inner void while the bar is at operating temperature, such that the bar is conformed to the cathode block.

Abstract: Extraction device designed to extract a short-circuiting wedge inserted between two conductors to take an electrolysis cell offline. The extraction device includes a means of gripping said wedge and, in conjunction with the upper horizontal face of each conductor, at least one jack directed vertically and including a body and a stem, a) stem (or said body) being interdependent with at least one horizontal support face directed downwards and laid out so that, when said extraction device is placed above said wedge to extract it, the upper horizontal face of each conductor is in line with one horizontal support face, b) said body (or said stem) being connected to said means of gripping said wedge, so that, when said jack is actuated to extract the wedge, said jack exerts opposing forces on said conductors and said wedge, tending to separate them.

Abstract: Frost-free surfaces and methods for manufacturing such surfaces are described. The frost-free surfaces reduce ice build-up, prevent vapor condensation and reduce adhesion force between ice and a solid substrate. The surfaces can be on parts used in devices where superhydrophobic properties may be obtained post or during device manufacturing. The superhydrophobic properties are the result of aluminum oxide clusters made on such surfaces.

Abstract: A method of preparing inorganic and/or organic surfaces includes organized micro- or nanostructures using colloidal particles in an electric field, to the micro- or nanostructured surfaces obtained by application of this method, as well as to the various applications of these structured surfaces, notably in the field of photonics, catalysis, magnetic storage or biosensors.

Abstract: Device for producing silicon blocks for photovoltaic applications, comprising a container for receiving a silicon melt with a base wall and at least one side wall, means for reducing the diffusion of impurities from at least one of the walls of the container into the silicon melt, wherein the means for reducing the diffusion of impurities comprise at least one covering element for the at least partial covering of at least one of the walls of the container.

Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

Abstract: A method of preparing a polysaccharide-protein fiber by preparing an aqueous solution comprising a polysaccharide and a protein, applying a high voltage to the solution, collecting the fiber on a collecting plate.

Abstract: A device for producing knitted fabric comprising a knitting machine and a roving drawing and strengthening unit. The roving drawing and strengthening unit has a roving supply unit, through which a roving can be provided in the form of a fiber bundle that has not been strengthened, a stretching unit, to which the roving can be fed in a conveying direction in the form of at least one sliver, and a spinning nozzle device, through which compressed air can be applied to a drawn roving exiting the stretching unit in a fiber strengthening segment.

Abstract: A crimping apparatus for crimping a filament bundle in a melt spinning process includes a conveyor nozzle and a stuffer box which is associated with the conveyor nozzle. For thermal processing, a processing unit, which includes a rotatable processing drum which, for guiding and temperature control of a thread plug, has a rotating drum wall, is disposed downstream of the stuffer box. In order to be able to carry out as gentle a processing of the thread plug as possible, the stuffer box is disposed axially parallel to the processing drum in such a manner that the thread plug can be infed in a straight run from a plug outlet of the stuffer box to the circumference of the drum wall. This allows the naturally acting weight force of the thread plug to be advantageously used for guiding the thread plug.

Abstract: An industrial two-layer fabric of 16 or more shafts consists of an upper side fabric having upper side warps and upper side wefts and a lower side fabric having lower side warps and lower side wefts, the upper side fabric and the lower side fabric are bound by binding yarns. A first warp pair consists of a binding yarn and one of an adjacent upper side warp, an adjacent lower side warp and an adjacent binding yarn. A second warp pair consists of an upper side warp and an adjacent lower side warp. In a complete design, two or more of the first warp pairs are placed adjacent to each other, and two or more of the second warp pairs are placed adjacent to each other.

Abstract: A feeder for a knitting machine includes a feeder arm with a dispensing area configured to feed a strand toward a knitting bed of the knitting machine. Moreover, the feeder includes an actuation arm that is operably coupled to the feeder arm. The actuation arm includes an abutment surface that is configured to abut against a drive bolt of the knitting machine to selectively move the feeder arm relative to the knitting bed. The abutment surface is rounded and convex.

Abstract: A non-crimp fabric includes at least two superimposed layers made from multifilament reinforcing yarns arranged parallel to each other, and at least one layer made from a non-woven made from thermoplastic polymer material on and/or between the layers made from multifilament reinforcing yarns, wherein the non-woven includes a first polymer component and a second polymer component whose melting temperatures lie below the melting or decomposition temperature of the reinforcing yarns. The first polymer component has a lower melting temperature than the second polymer component, and the first polymer component is soluble in epoxy matrix resins, cyanate ester matrix resins, benzoxazine matrix resins, or mixtures thereof. The second polymer component is not soluble in epoxy matrix resins, cyanate ester matrix resins, benzoxazine matrix resins, or mixtures thereof. A preform is made from the non-crimp fabric.

Abstract: A method for manufacturing a conductive fabric is undertaken via a fixing device which has a plurality of working sections. According to an arrangement of the conductive yarn disposed between a first flexible base and a second flexible base, the working sections of the fixing device are extended to form a plurality of fixing points and the other working sections are retracted without forming the fixing points, thereby fixing the conductive yarn between the first and second flexible bases with a dot-like spaced pattern to obtain conductive fabric products having different conductive yarn arrangements without replacing the fixing device.