Abstract: A method for mineralizing siloxane derivatives from a liquid organic phase by adding a base in the form of an alkaline hydroxide.

Abstract: A polymer-brush-based, surface-modification strategy for friction and wear reduction in hard contact under boundary-lubrication conditions is proposed, specifically for a non-aqueous environment. Surface-initiated Atom Transfer Radical Polymerization (SI-ATRP) was employed for the synthesis of three different oil-compatible, hydrophobic polymer brushes based on alkyl methacrylates. This study presents polymerization kinetics, chemical characterization by means of Fourier transform infrared spectroscopy (FTIR) and surface morphologies observed in atomic force microscopy (AFM). The lubrication properties of the anchored polymers were evaluated macroscopically by means of ball-on-disk methods and on the nanonewton scale by lateral force microscopy (AFM/LFM) and showed significant reduction in friction up to contact pressures as high as 460 MPa. The frictional response of surface-grafted polymers is shown to depend strongly on the compatibility of the polymer with the chosen lubricating fluid.

Abstract: This invention relates to carbon-based materials as anti-friction and anti-wear additives for advanced lubrication purposes. The materials comprise carbon nanotubes suspended in a liquid hydrocarbon carrier. Optionally, the compositions further comprise a surfactant (e.g., to aid in dispersion of the carbon particles). Specifically, the novel lubricants have the ability to significantly lower friction and wear, which translates into improved fuel economies and longer durability of mechanical devices and engines.

Abstract: A silicon-wafer processing fluid used for processing a silicon wafer contains a friction modifier containing a nitrogen-containing compound, pH of the nitrogen-containing compound being in a range from 2 to 8 when a mass ratio with water (i.e. nitrogen-containing compound/water) is 1/99. The nitrogen-containing compound is preferably a heterocyclic compound. The silicon-wafer processing fluid restrains an abrasion of abrasive grains rigidly attached to a wire and generation of hydrogen.

Abstract: This invention relates to anthranilate esters and their use in lubricants, such as engine oils. The invention particularly relates to compositions that deliver an ash-free base to a lubricant in the form of a basic amine additive, without adversely impacting seal compatibility and/or degradation, and methods thereof.

Abstract: A friction modifying lubricant additive is provided comprising a base oil, colloidal nanocarbon particles, and a fluorine containing oligomeric dispersant. The fluorine containing oligomeric dispersant includes an anchoring group, a lipophilic hydrocarbon group, and a fluorinated oleophobic group. Further, a friction modifying lubricant additive is provided comprising a base oil, colloidal nanocarbon particles, a fluorine containing oligomeric dispersant, and at least one component selected from the group consisting of an antifriction component, an antiwear component, and an extreme pressure component. In another aspect, a method of manufacturing a lubricant additive is provided, the method comprising the step of mixing together a fluorine containing oligomeric dispersant, a dispersion of colloidal nanocarbon particles in a first base oil, and a second base oil.

Abstract: A piston coating is described that comprises a phenolic resin, at least one solid lubricant selected from among the group including graphite, MoS2, WS2, BN, and PTFE, as well as carbon fibers. Said coating has an advantageous wear resistance and an advantageous coefficient of friction.

Abstract: A novel lubricating grease that is useful for underwater applications is made up of lubricating oil that contains a co-polymer of a hydrocarbon backbone for promoting good adhesion and a fluorine containing backbone for promoting lubricity. The grease formulation is resistant to water washout and does not off-gas toxic compounds. In addition to the lubricating oil, the grease formulation includes fumed silica, and may contain one or more corrosion inhibitors, an extreme pressure filler such as boron nitride, and optionally one or more polyurethane initiators.

Abstract: A floral fragrance composition for use as a replacement for 3-(4-tertbutylphenyl)-2-methylpropanal (LILIAL®) that is a primary perfuming ingredient composition that includes a first perfuming ingredient of (2,5-dimethyl-2,3-dihydro-IH-inden-2-yl)methanol (LILYFLORE®) in an amount of 5 to 25% by weight; a second perfuming ingredient of a dimethyl-5-indanyl propanal compound in an amount of 0.2 to 10% by weight; and one or more secondary perfuming ingredients including at least one of 3-methyl-5-phenylpentanal, decanal, octanal, cuminic aldehyde, cuminic alcohol, carvone, tetrahydro-2-isobutyl-4-methyl-4(2H)-pyranol, or cis-7-P-menthanol.

Abstract: The present application relates to perfume delivery systems and consumer products comprising perfume delivery systems and or perfume raw materials, as well as processes for making and using such perfume delivery systems and consumer products.

Abstract: The candle uses wax beads produced using existing equipment. The wax beads are transported to a bead hopper and the bead hopper deposits a volume of wax beads into jars prepared with a wick. Concurrently the jars are vibrated using a continuous vibratory motor to settle the wax beads for increased wax bead density. The filled jars are then transported to an in-container wax bead compression system where an automated plunging device compresses the wax beads. Jars are then transported by in-line conveyor to a wide conveyor. The jars are then filled at a liquid wax depositing station where the wax has been heated to its melting point and deposited into the jar to fill the void left by the in-container wax bead Compression system. The filled candle is then cooled to solidification. The finished candle is then ready for packing, distribution, sale, and use.

Abstract: Reacting at least one tris-(2-hydroxyethyl)-amine fatty acid ester with dimethylsulfate at a molar ratio of dimethylsulfate to amine nitrogen of from 0.79 to 0.94 until the reaction mixture has a total amine number of from 7 to 20 mg KOH/g provides novel fabric softener active compositions with a low content of methanol, comprising from 65 to 98% by weight of tris-(2-hydroxyethyl)-methylammonium methylsulfate fatty acid esters and from 1 to 1500 ppm methanol.

Abstract: A fabric conditioning composition comprising an emulsion of particles in an aqueous vehicle, the particles comprising (a) fatty acid triglyceride, and (b) a water swellable cationic polymer.

Abstract: A dishwasher detergent composition, preferably pH neutral, and comprising a strong biodegradable builder and optionally a bleach, and optionally a sulfonated polymer.

Abstract: A solid composition for treating cooling or heated water systems to remove scale, microorganisms and biofilm, and corrosion by-products. The composition for cooling towers and chilled water systems comprises chelating agents and an anionic surfactant. The composition for boilers and heated water systems comprises a sulfite, neutralizing amines, sodium phosphate, and, optionally, a polymethacrylate or polyacrylate polymer. A method for using such a treatment composition comprises contacting the treatment composition with substantially all parts of the water system.

Abstract: A solid-state fermentation method is provided. The method is provided for edible and medicinal microorganisms. A commensurate device is used for processing sterilization, inoculation, cultivation, drying, and collection. Cultivation substrates are filled in cultivation plates and placed on trays of a movable layer rack in a reaction fermenter for fermentation. Inner pipes provide inoculation, fluid nutrient, and air for fermentation of microorganisms. The reaction fermenter is accompanied with a dry circulation unit and a production collection unit for drying and collection. The solid-state fermentation processes are thus effectively integrated without the need of transferring fermented products between stations, which largely reduces contamination rate. Even more, there is no need to purchase automatic production equipments of high mechanical technologies.

Abstract: Gas permeable devices and methods are disclosed for cell culture, including cell culture devices and methods that contain medium at heights, and certain gas permeable surface area to medium volume ratios. These devices and methods allow improvements in cell culture efficiency and scale up efficiency.

Abstract: By maintaining the cell density during culture at an appropriate level and eliminating the procedure of transferring cells from a container to another container when a large amount of cells are cultured, damage on cells can be reduced, risk of contamination can be lowered, labor saving can be attained and automation can be attained. A cell culture apparatus including: a culture container 11 formed of a soft packing material and having an enclosure part 11-1 in which a culture medium and/or cells being cultured are enclosed is placed; a container table 12 on which the culture container 11 is placed; a roller 13 which is placed on the upper surface of the culture container 11 and divides the enclosure part 11-1 into two or more chambers; and a driving means 15 which relatively moves the roller 13 or the culture container 11, thereby to change the volume of the culture part 11-11 in the enclosure part 11-1 in which a culture medium or cells being cultured are enclosed.

Abstract: A device 2 for producing a three-dimensional structural body has an accommodating plate 3 in which an accommodating recess portion 3a for accommodating a cell mass 1 is formed, a support body 4 provided with a plurality of needle-shaped bodies 6 penetrating the cell mass, a suction nozzle 19 for adsorbing/holding the cell mass, and structure 20 for moving the suction nozzle.

Abstract: A cell culture system for the production of cells and cell derived products includes a reusable instrumentation base device incorporating hardware to support cell culture growth. A disposable cultureware module including a cell growth chamber is removably attachable to the instrumentation base device. The base device includes microprocessor control and a pump for circulating cell culture medium through the cell growth chamber. The cultureware module is removably attached to the instrumentation base device. Cells are introduced into the cell growth chamber and a source of medium is fluidly attached to the cultureware module. Operating parameters are programmed into the microprocessor control. The pump is operated to circulate the medium through the cell growth chamber to grow cells or cell products therein. The grown cells or cell products are harvested from the cell growth chamber and the cultureware module is then disposed.

Abstract: An apparatus (100) capable of viral inactivation of liquid media includes at least one coaxial cylinder (110) constructed of an outer cylinder (120) and an inner cylinder (130), a liquid media inlet (140), at least one emitter of type C ultraviolet radiation (145), and a liquid media outlet (150). The inner cylinder has an outer diameter adapted to form a gap (160) between the outer diameter of the inner cylinder and the inner diameter of the outer cylinder. The media flows in a substantially cyclonic flow path along the gap. The at least one emitter of type C ultraviolet radiation is placed inside the inner cylinder. The outlet is connected to the outer cylinder at, or proximal to, an end of the outer cylinder opposite the inlet.

Abstract: Methods of inducing or increasing lipid and protein production in microalgae are disclosed. Methods of enhancing microalgae biomass are also disclosed. The methods may comprise inoculating a culture media with microalgae and propagating the microalgae under heterotrophic growth conditions. The heterotrophic growth conditions may comprise inhibiting exposure of the inoculated culture media to light. The methods may further comprise delivering oxygen into the inoculated culture media. Additionally, lipids, proteins, and other cellular components may be isolated and purified from microalgae cultivated under the disclosed methods.

Abstract: Compositions of purified biologically active Wnt proteins are provided. Wnt proteins are found to be hydrophobic and post-translationally modified by addition of a lipid moiety at a conserved cysteine residue. Methods for isolation of Wnt utilize detergents that maintain the solubility of the modified protein.

Abstract: Disclosed are cells exhibiting neuronal progenitor cell characteristics, and methods of making them from marrow adherent stem cells by regulating cellular pathways in the marrow adherent stem cells that are associated with glial transdifferentiation of the marrow adherent stem cells.

Abstract: The invention provides compositions and methods for the preparation of biocompatible biomaterials from adipose tissue. Biocompatible biomaterials are cellular or acellular biomaterials. The invention further provides methods of use of the biocompatible biomaterials.

Abstract: A method of culturing mesenchymal stem cells is provided. The mesenchymal stem cells are cultured in a medium that includes a cell-depleted Wharton's Jelly filtrate. The cell-depleted Wharton's Jelly filtrate is prepared by mincing umbilical cord tissue that includes Wharton's Jelly, subsequently diluting the umbilical cord tissue, subsequently filtering the umbilical cord tissue to generate a filtrate, and sedimenting Wharton's Jelly stem cells from the filtrate to generate the cell-depleted Wharton's Jelly filtrate.

Abstract: This invention relates to methods to stabilize and/or improve the function of parenchymal cells. Also provided are systems of co-cultures of hepatocyte-stabilizing non-parenchymal cells used in bioreactor microenvironments to identify hepatic stabilizing factors by gene-expression profiling.

Abstract: A medium is described for the protein-free and serum-free cultivation of cells, especially mammalian cells, whereby the medium contains a proportion of soy hydrolysate.

Abstract: Genetically modified bacteriophage and methods of using the same to detect bacterial types of interest are provided.

Abstract: Reverse engineering has offered new ways of studying the pathology of RNA viral infections, new more efficient devices of synthesizing recombinant viruses and developing vaccines and also demonstrated the versatility and efficiency of RNA dependent RNA polymerase RDRP system as an expression system. However, the currently used methods require a repertoire of complex, difficult-to-use tools. Present invention describes, a simpler plasmid based mammalian expression system that uses the RDRP enzyme activity for expression of recombinant proteins or RNA from viral minigenomes and rescue of recombinant viruses from cDNAs encoding entire genome(s) of negative stranded RNA viruses. This system will be useful for expression of recombinant proteins, therapeutic RNA molecules including anti-sense and/or selecting interfering RNA and Ribozymes. This system can also be used for gene therapy and producing recombinant viruses for production of new vaccines.

Abstract: This invention relates to a HEK293 cell line that grows under animal component-free suspension conditions. The cell line is ideal for rapid and scalable production of adeno-associated virus (AAV) and supports production of all serotypes and chimera of AAV.

Abstract: Animal cells are described which can be infected by viruses and which are adapted to growth in suspension in medium free of animal-derived components, such as serum-free medium. Processes for the replication of viruses in cell culture using these cells are furthermore described, as well as vaccines which contain the viruses or antigenic portions thereof obtainable by the process.

Abstract: The present invention relates to methods and compositions for improving the productivity of recombinant vitamin K dependent protein expression in host cells.

Abstract: Recombinant microbial cells are disclosed herein that comprise (i) a down-regulation of an endogenous polynucleotide sequence encoding Sou2 sorbitol utilization protein, and (ii) a polyunsaturated fatty acid (PUFA) biosynthetic pathway. The down-regulation of the polynucleotide sequence encoding Sou2 sorbitol utilization protein can increase the lipid content of the microbial cells and/or decrease the total amount of sugar alcohols produced by the microbial cells. Also disclosed are methods of using the recombinant microbial cells to produce oil containing omega-3 polyunsaturated fatty acids such as EPA.

Abstract: The present invention relates to a method of reducing the level of uric acid in a subject, which comprises administering to said subject an effective amount of a fermentation product of Tenacibaculum sp. Also provided are a method of preventing and/or treating a disease or disorder related to hyperuricemia, a method of increasing the digestion of uric acid and a method of producing uricase.

Abstract: The present invention relates to nucleic acid and amino acid sequences from Akkermansia muciniphila and from Bacteroides fragilis, coding for/representing novel alpha-1,3-fucosyltransferases. The invention also provides uses and methods for using the alpha-1,3-fucosyltransferases to generate fucosylated products, such as oligosaccharides, (glyco)proteins, or (glyco)lipids, in particular of 3-fucosyllactose.

Abstract: Disclosed are methods and compositions related to ONC-T18, D4-desaturases, D5 elongases, their isolation, characterization, production, identification, and use for fatty acid production, as well as organisms containing these compositions and organisms expressing them.

Abstract: Described herein are molecules, constructs and methods for the production and secretion of polypeptides of interest by host cells, preferably bacterial host cells, and more particularly gram positive bacteria. In particular, the present invention is related to a polynucleic acid encoding a fusion protein and to uses thereof for the secretion of heterologous or homologous polypeptides of interest by a bacterial host cell, preferably Clostridium bacteria. The present invention further relates to methods and constructs for the production and secretion of heterologous or homologous polypeptides of interest proteins by host cells using such polynucleic acids and fusion proteins.

Abstract: The invention relates to a polypeptide comprising the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 96% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 96% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.

Abstract: The present invention relates to isolated polypeptides having protease activity, and the use of isolated polypeptides having protease activity in animal feed. It also relates to the use of isolated nucleic acid sequences encoding the proteases in the recombinant production of isolated polypeptides having protease activity and isolated nucleic acid sequences encoding the proteases. The invention also relates to nucleic acid constructs, vectors, and host cells, including plant and animal cells, comprising the nucleic acid sequences, as well as methods for producing and using the proteases, particularly using the proteases in animal feed.

Abstract: The present invention provides culture mediums that are useful for the expression of ADAMTS proteins, such as ADAMTS13. Methods for the expression and purification of ADAMTS proteins are also provided. In some embodiments, the mediums and methods of the invention are useful for the expression of ADAMTS proteins having high specific activities. Also provided are ADAMTS, e.g., ADAMTS13, protein compositions with high specific activities, which are expressed and purified according to the methods provided herein.

Abstract: Isolated polynucleotides and polypeptides and recombinant DNA constructs of plastidial sugar epimerases useful for conferring improved agronomic performance including yield and drought are disclosed. Compositions (such as plants or seeds) having these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs are also disclosed.

Abstract: A salt tolerant Medicago sativa plant designated as “Silverosa” representative seed of which having been deposited under NCIMB Accession No. 41909, NCIMB Accession No. 41910 and NCIMB Accession No. 41911.

Abstract: A system and method for processing and detecting nucleic acids from a set of biological samples, comprising: a capture plate and a capture plate module configured to facilitate binding of nucleic acids within the set of biological samples to magnetic beads; a molecular diagnostic module configured to receive nucleic acids bound to magnetic beads, isolate nucleic acids, and analyze nucleic acids, comprising a cartridge receiving module, a heating/cooling subsystem and a magnet configured to facilitate isolation of nucleic acids, a valve actuation subsystem configured to control fluid flow through a microfluidic cartridge for processing nucleic acids, and an optical subsystem for analysis of nucleic acids; a fluid handling system configured to deliver samples and reagents to components of the system to facilitate molecular diagnostic protocols; and an assay strip configured to combine nucleic acid samples with molecular diagnostic reagents for analysis of nucleic acids.

Abstract: A method comprises: sorbing a sample solution comprising nucleic acids to a sample receiving portion of a quartz fiber filter by contacting the sample solution with the sample receiving portion; and washing the sample receiving portion while keeping most of nucleic acids around the sample receiving portion by flowing a wash solution through the sample receiving portion under a wicking force directed away from the sample receiving portion. An associated apparatus is also provided.

Abstract: Compositions and methods for modulating the expression of a protein of interest are provided.

Abstract: The invention relates to the diagnostic and therapeutic uses of a miRNA molecule, an equivalent or a source thereof in a disease and condition associated with neo-angiogenesis.

Abstract: A method that includes measuring the expression level of at least one transposon in a biological sample from a subject; and determining whether the measured transposon expression exceeds a predetermined level, and if so, administering to the subject a transposon inhibitor in an amount effective to reduce the expression level of a transposon.

Abstract: Long noncoding RNAs (lncRNAs) are identified that enhance pluripotency reprogramming of somatic cells as well as differentiation of pluripotent cells. Induced pluripotent stem (iPS) cell generation from somatic cells leads to the upregulation and downregulation of identified lncRNAs. The modulation of these lncRNAs are capable of enhancing pluripotency of somatic cells as well as enhancing differentiation of a pluripotent cell.

Abstract: The invention relates to a double-stranded ribonucleic acid (dsRNA) for inhibiting the expression of Factor V, comprising an antisense strand having a nucleotide sequence which is less that 25 nucleotides in length and which is substantially complementary to at least a part of Factor V. The invention also relates to a pharmaceutical composition comprising the dsRNA together with a pharmaceutically acceptable carrier; methods for treating diseases caused by the expression of Factor V using the pharmaceutical composition; and methods for inhibiting the expression of Factor V in a cell.