Abstract: An apparatus for collecting kerogen contained in oil shale in a form of intermediate oil using an alternate operating scheme, and collecting method using the same comprising: a) installing a heating coil and a heating jacket through which a heat carrier passes and a support for supporting the heating coil, in a body of an extraction device, b) heating and stiffing sludge containing kerogen decomposition intermediate in a middle storage tank again, c) collecting an extraction agent or low boiling point oil discharged and reusing the collected extraction agent or oil in the middle storage tank, d) separating the sludge discharged from the middle storage tank into an intermediate oil/extraction agent mixture oil and residual sludge through a sludge separation device, and e) separating the separated mixture oil into the extraction agent and the intermediate oil by a separation distillation column and preheating and reusing the separated extraction agent.

Abstract: Disclosed are catalyst compositions including zeolite and silica components, methods of making, and processes of using in the thermo-catalytic conversion of biomass. Such disclosed methods of making include: i) spray drying of the catalyst precursor slurry at a pH below 1, or ii) the removal of ions such as sodium from the binder material prior to spray drying the catalyst precursor slurry at a pH below 2.7, or iii) spray drying the catalyst precursor including a pore regulating agent followed by steam treating, or iv) some combination of i), ii) and iii).

Abstract: A process is disclosed for recovering hydroprocessing effluent from a hydroprocessing unit utilizing a hot stripper and a cold stripper. Only the hot hydroprocessing effluent is heated in a fired heater prior to product fractionation, resulting in substantial operating and capital savings.

Abstract: The present invention relates to demulsifying and dehydrating formulations of heavy crude oil based block copolymers amine bifunctionalized with low polydispersities. These formulations can contain solvents whose boiling point is in the range from 35 to 200° C., preferably: dichloromethane, chloroform, toluene, xylenes, turbosine, naphtha or mixtures thereof.

Abstract: A hydroisomerization catalyst of the present invention is obtained by calcining a catalyst composition containing an ion-exchanged molecular sieve or a calcined product thereof, and at least one metal selected from the group consisting of metals of Groups 8 to 10 in Periodic Table of the elements, molybdenum and tungsten, carried on the ion-exchanged molecular sieve or the calcined product thereof, wherein the ion-exchanged molecular sieve is obtained by ion-exchanging a molecular sieve, which includes nanocrystals having a pore structure of ten-membered rings or eight-membered rings and having a ratio of the pore volume to the external surface area ([pore volume]/[external surface area]) of 2.0×10?4 mL/m2 to 8.0×10?4 mL/m2 and contains an organic template, in a solution containing a cationic species.

Abstract: The present invention relates to a bifunctional catalyst for a hydrodewaxing process with improved isomerization selectivity, and to a method for manufacturing the same, and more particularly to a bifunctional catalyst and to a method for manufacturing same, which is characterized in that EU-2 zeolite with a controlled degree of phase transformation is used as a catalyst support having an acid site. The EU-2 zeolite, the degree of phase transformation of which is controlled, includes, by controlling synthesis parameters of EU-2, predetermined amounts of materials that are phase-transformed from EU-2 crystals such as cristobalite and quartz. The metal loaded bifunctional catalyst according to the present invention improves selectivity of the isomerization process, rather than a cracking reaction, during a hydroisomerization reaction of n-hexadecane. Therefore, the bifunctional catalyst can be widely used as a catalyst for a dewaxing process such as lubricant base oil and diesel oil.

Abstract: Methods and systems for converting petroleum based oil into fuel generally include forming a conversion mixture of an alcohol and a base, adding the conversion mixture to petroleum based oil (such as crude oil, used oil, used motor oil, and new motor oil) to form a reaction mixture, adding a high nitrate compound and an amino acid the reaction mixture, ozonizing the reaction mixture. The result may include a three phase system in which the bottom phase is asphalt oil, the middle phase is diesel fuel or jet fuel, and the top phase is sulfuric acid. The three phases may be separated to obtain the final diesel fuel or let fuel product. Additional cooling and/or filtering processes may be utilized to complete and/or optimize oil conversion.

Abstract: A microwave plasma based entrained flow gasifier of biomass, including a furnace body and a fuel pretreatment system. The furnace body includes a fuel inlet disposed at the lower part of the furnace body, a syngas outlet disposed at the top of the furnace body, and a slag outlet disposed at the bottom of the furnace body. The fuel inlet presents in the form of nozzles. The fuel pretreatment system is disposed outside of the furnace body, and includes a fuel crushing apparatus, a sieving apparatus disposed downstream to the fuel crushing apparatus, a first fuel container for receiving particle size-qualified fuel, a second fuel container for receiving particle size-unqualified fuel, and a feeding hopper disposed downstream to the first fuel container. The first fuel container and the second fuel container are disposed side-by-side downstream to the sieving apparatus.

Abstract: Novel redox based systems for fuel and chemical production with in-situ CO2 capture are provided. A redox system using one or more chemical intermediates is utilized in conjunction with liquid fuel generation via indirect Fischer-Tropsch synthesis, direct hydrogenation, or pyrolysis. The redox system is used to generate a hydrogen rich stream and/or CO2 and/or heat for liquid fuel and chemical production. A portion of the byproduct fuels and/or steam from liquid fuel and chemical synthesis is used as part of the feedstock for the redox system.

Abstract: Integrated processes are provided for syngas refining and bioconversion of syngas to oxygenated organic compound. In the integrated processes ammonia contained in the syngas is recovered and used as a source of nitrogen and water for the fermentation. The integrated processes first remove tars from syngas by scrubbing using a first aqueous medium under conditions that ammonium bicarbonate is unstable. With tars removed, contact between the syngas and a second aqueous medium enables ammonia and carbon dioxide to be removed from the syngas without undue removal of components adverse to the fermentation, processing or oxygenated product such as benzene, toluene, xylene, ethylene, acetylene, and hydrogen cyanide. At least a portion of the second aqueous medium is supplied as a source of water and ammonia for the fermentation.

Abstract: To provide a catalyst for production of a biodiesel fuel, which is capable of producing a biodiesel fuel with high selectivity, at a high yield, and at a low cost even in the presence of a water content and a free fatty acid, and a biodiesel fuel production method using the catalyst. Use of a titanium-containing mesoporous silica containing Ti and Si as skeleton constituent elements and having a pore diameter of 5 nm or greater as a transesterification catalyst enables a biodiesel fuel to be produced with high selectivity at a high yield through transesterification between an oil and an alcohol.

Abstract: A process for the removal of sulfur compounds from a feed stream. A first separation zone removes sulfur compounds and produces a partially cleaned stream. A first adsorption zone adsorbs the remaining organic sulfur compounds on a regenerable adsorbent a produces a treated gas stream. A portion of the treated gas stream may regenerate the adsorbent in the first adsorption zone by removing organic sulfur compounds. The organic sulfur compound rich stream can be passed to a non-regenerable adsorption zone. The non-regenerable adsorption zone will separate out the organic sulfur compounds and provide a re-treated gas stream which may be recycled to a portion of the process. The non-regenerable adsorption zone may include regenerable adsorbent, but the zone is not operated to regenerate the adsorbent while it is in the non-regenerable adsorption zone.

Abstract: A method for treating liquefied hydrocarbons including acid gases to remove said acid gases while minimizing loss of amine species, he method comprising the step of contacting he liquefied hydrocarbons with an absorbent aqeuous solution of a first amine compound, the first amine compound having the structure: wherein R1 is hydrogen or propane-2,3-diol and R2 is propane-2,3-diol, or mixtures thereof.

Abstract: A process for cleaning and dewatering hydrophobic particulate materials is presented. The process is performed in two steps: 1) agglomeration of the hydrophobic particles in a first hydrophobic liquid/aqueous mixture; followed by 2) dispersion of the agglomerates in a second hydrophobic liquid to release the water trapped within the agglomerates along with the entrained hydrophilic particles.

Abstract: The invention relates to thiophosphates and derivatives thereof useful as antiwear additive components, lubricant additive compositions and lubricant compositions each comprising such compounds, methods for making and using the same, including methods of lubricating machines and machine parts and methods of extending the useful life of elastomeric seal components of such machines. In one aspect, the invention relates to a lubricant additive composition comprising a compound of formula (I): or a tribologically acceptable salt thereof, and a sulfonate or a phenate detergent.

Abstract: Anti-wear and friction-reducing lubricants and additives to lubricants for both ferrous and non-ferrous materials with/without DLC (diamond-like-coatings) or graphene-based coatings, which are halogen free boron based ionic liquids comprising a combination of an anion chosen from a mandelato borate anion, a salicylato borate anion, an oxalato borate anion, a malonato borate anion, a succinato borate anion, a glutarato borate anion and an adipato borate anion, with at least one cation selected from a tetraalkylphosphonium cation, a choline cation, an imidazolium cation and a pyrrolidinium cation, wherein said at least one cation has at least one alkyl group substituent with the general formula CnH2n+1, wherein 1?n?80. Advantages of the invention include that it provides halogen free ionic liquids for lubrication and that sensitivity for hydrolysis is reduced.

Abstract: A lubricant composition is disclosed. The lubricant composition is made up of (a) an API Group III base stock; (b) one or more semi-crystalline viscosity modifier; and (c) one or more LOFIs having a side-chain distribution which satisfies the following requirements: (1) the distribution contains side chains ranging from C8 to C18 with an average carbon number ranging from 12.4 to 14.4; (2) the side chain distribution is bi-modal with a lower portion of the bi-modal distribution made up primarily of C12 and an upper portion of the distribution made up primarily of C16, C18 or combinations thereof; (3) the total mole % of the upper portion of the distribution must be less than that of the lower portion of the distribution; and (4) the amount of C12 on the side chain must be at least 40 mole % of the total side chain distribution.

Abstract: Compositions and uses thereof to clean and/or rinse tableware, wherein the compositions include a sulfonated silane.

Abstract: The present invention relates to cleaners for hard surfaces which comprise surfactants and also phosphoric acid esters of a polyether-modified alkyl alcohol, and to the method of use thereof for cleaning hard surfaces and for generating shine on a hard surface.

Abstract: The present composition relates to fabric care compositions comprising an organosiloxane polymer. Methods of using such compositions including contacting a fabric with the composition and rinsing the fabric are also disclosed.

Abstract: An optofluidic photobioreactor including an optical waveguide having an input, characterized by an evanescent optical field confined along an outer surface of the optical waveguide produced by radiation propagating in the optical waveguide, means for inputting light to the input of the optical waveguide, and a selected photosynthetic microorganism disposed substantially within the evanescent field. A method for optically exciting a photosynthetic microorganism for generating a biofuel, a biofuel precursor, or a biomass from the optically-excited photosynthetic microorganism involves irradiating the photosynthetic microorganism attached to the surface of the waveguide with an evanescent optical field from optical radiation propagating in the optical waveguide, and driving photosynthesis in the microorganism by the evanescent optical field.

Abstract: A carrier for expansion of pluripotent stem cells is provided, wherein the carrier comprises a substrate comprising one or more outer surfaces, wherein the one or more outer surfaces are modified with gas plasma treatment, and one or more structured indentations on one or more of the outer surfaces. The carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.05 mm to 1.2 mm and each of the structured indentations has a major axis in a range from about 0.1 mm to 0.5 mm, a minor axis in a range from about 0.1 mm to 0.5 mm and a depth in a range from about 0.025 mm to about 0.5 mm. A method of making the carrier, and culturing stromal cells using the same carrier are also provided.

Abstract: The present invention relates to hematopoietic cells, and more specifically to methods for long-term in vitro culturing and ex vivo expansion of hematopoietic cells. The present invention also provides compositions useful for culturing cells, such as media for culturing hematopoietic cells, specifically haematopoietic stem cells (HSC) and haematopoietic progenitor cells (HPC). The present invention further provides compositions including growth factor combinations and methods utilizing altered growth and environmental conditions that are applicable in vitro culturing and to ex vivo expansion of HSC and/or HPC.

Abstract: A method for enhancing the therapeutic effect of stem cells on autoimmune diseases, cardiovascular diseases, and/or hematological diseases is provided. The method comprises pre-treating the stem cells with ligustilide to increase the expression of differentiation-promoting genes in the stem cells, increase the expression of homing-promoting genes in the stem cells, and/or decrease the expression of inflammatory genes in the stem cells, wherein the treatment is conducted in a culture medium of the stem cell.

Abstract: The present invention relates to a novel Listeria bacteriophage designated ProCC P825. In particular, the present invention relates to the endolysin PlyP825 encoded by the novel phage ProCC P825 and uses of the novel endolysin PlyP825 for controlling Listeria contamination and infection.

Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: The invention is directed to compositions to screen for small molecule drugs that inhibit proteases, such as viral proteases, e.g., HIV proteases; and methods for making and using these compositions. The invention provides compositions and methods for identifying compositions, e.g., drug molecules, that can inhibit proteases, e.g., HIV proteases. In alternative embodiments, the invention provides cell-based assays to screen for compositions, e.g., small molecules or drugs, that inhibit or modify the activity of enzymes such as calcium-dependent protein convertases involved in HIV envelop protein processing, including cleavage of the HIV gp160 envelope precursor, resulting in gp120 and gp41 envelope products.

Abstract: The present invention relates to the development of new derivatives of a bacterial plasminogen activator, Staphylokinase (SAK), having one or more amino acid residues with single or multiple cysteines at the amino and/or carboxy terminal ends and their conjugation with PEG (Polyethylene Glycol), resulting in new Staphylokinase derivatives that display altered oligomeric states, enhanced thermal and protease stability and extended plasma half-life. Also included is the cloning and expression in a suitable bacterial host; purification of Staphylokinase derivatives to homogeneity and their chemical modification by integrating a PEG molecule to create new biologically active Staphylokinases having higher protein stability and improved in vivo plasma half life, that may enhance the clinical potential of Staphylokinase in thrombolytic therapy for the treatment of cardiovascular diseases.

Abstract: The invention relates to a tomato plant the fruits of which have an improved shelf-life as compared to the fruits of a wild type tomato plant, obtainable by introgressing the increased shelf life trait from the mutant LePQ58 (deposit accession number NCIMB 41531) into a tomato plant with a normal shelf-life. The increased shelf-life may comprise a fruit that shows normal ripening having a fruit firmness at red ripe harvest that is increased by at least 31%, preferably by at least 42%, more preferably by at least 52%, even more preferably by at least 60%, most preferably by at least 70% as compared to a fruit having similar genetic background that lacks the trait of the invention.

Abstract: A method for efficiently producing homozygous organisms from a heterozygous non-human starting organism, comprising providing of a heterozygous starting organism; allowing the starting organism to produce haploid cells; creating homozygous organisms from the haploid cells thus obtained; and selecting the organisms having the desired set of chromosomes; wherein during production of the haploid cells no recombination occurs in order to obtain a limited number of genetically different haploid cells. Recombination can also be prevented or suppressed.

Abstract: The current invention relates to a method for targeted alteration of acceptor DNA, for example duplex acceptor DNA. The method comprises use of at least two oligonucleotides, each oligonucleotide having at least one mismatch relative to the targeted (duplex) acceptor DNA. The mismatch of the first oligonucleotide is directed to a nucleotide at a position in the first strand of the duplex and the mismatch of the second oligonucleotide is directed to the nucleotide in the second strand that occupies the complementary position in the duplex acceptor DNA (e.g. forms a base-pair with the nucleotide in the first strand). These mismatches are located at specific positions within said oligonucleotides. Also provided is a kit that comprises instructions for performing the method according to the inventions, and in a preferred embodiment, comprises oligonucleotides suitable for use in the method.

Abstract: Provided herein are antisense compounds and methods for recruiting one or more non-cleaving protein to a target nucleic acid in a cell. In certain instances such recruitment of a non-cleaving protein alters the function or activity of the target nucleic acid. In certain such instances, the target nucleic acid a pre-mRNA and the recruitment of the non-cleaving protein results in a change in splicing of the pre-mRNA.

Abstract: The disclosure relates to an RNA interference (RNAi) agent and the use of that RNAi agent to treat chronic pain in individuals, as well as pharmaceutical compositions containing the RNAi agents of the invention. The DNA-directed RNA interference (ddRNAi) agent for inhibiting expression of one or more target sequences in a pain associated gene comprises, one or more effector sequence sequences and effector complement sequences of at least 17 nucleotides in length, wherein the effector sequence is substantially complementary to the predicted transcript of a target sequence within a pain associated gene.

Abstract: Disclosed herein are methods and compounds for inhibiting gene expression by inhibiting enhancer RNAs (eRNAs). Such methods and compounds are useful for reducing expression of certain genes, many of which are associated with a variety of diseases and disorders.

Abstract: The invention is directed to compositions and methods for selectively reducing the expression of a gene product from a desired target gene in a cell, as well as for treating diseases caused by the expression of the gene. More particularly, the invention is directed to compositions that contain double stranded RNA (“dsRNA”), and methods for preparing them, that are capable of reducing the expression of target genes in eukaryotic cells. The dsRNA has a first oligonucleotide sequence that is between 25 and about 30 nucleotides in length and a second oligonucleotide sequence that anneals to the first sequence under biological conditions. In addition, a region of one of the sequences of the dsRNA having a sequence length of at least 19 nucleotides is sufficiently complementary to a nucleotide sequence of the RNA produced from the target gene to trigger the destruction of the target RNA by the RNAi machinery.

Abstract: The invention provides compositions and methods for signal activated RNA interference (saRNAi), preferably in vivo. The invention provides polynucleotides that switches between an inactive form and an active form upon covalent or non-covalent interaction with one or more specific chemical signals, such as disease-specific mRNA, miRNA, or other cellular RNA products with sequences that characterize diseased states of the cell. The interaction between the subject polynucleotides and the signals is preferably mediated by hybridization, which exposes, facilitates the formation, and/or allows the formation of a substrate that can be processed by proteins of the RNAi pathway (such as Dicer). The input and output of multiple different polynucleotides of the invention can form an in vivo signaling network. In addition, the multiple input signals can be integrated to modulate the activity of the subject polynucleotides.

Abstract: The invention relates to antisense oligonucleotidic sequences (ODN) against Smad7 suitably modified, and their uses in medical field as therapeutic biological agents, in particular in the treatment of chronic inflammatory bowel disease, such as Crohn's disease and ulcerative colitis.

Abstract: The present invention is related to a nucleic acid molecule capable of binding to human C5a, wherein the nucleic acid molecule comprises a central stretch of nucleotides, wherein the central stretch of nucleotides comprises a nucleotide sequence of 5? AUGn1GGUGKUn2n3RGGGHUGUKGGGn4Gn5CGACGCA 3? [SEQ ID NO: 61], wherein n1 is U or dU, n2 is G or dG, n3 is A or dA, n4 is U or dU, n5 is U or dU and G, A, U, C, H, K, and R are ribonucleotides, and dU, dG and dA are 2?-deoxyribonucleotides.

Abstract: The present invention provides polynucleotides and related polypeptides of the ZmARGOS gene family. The invention provides genomic sequence for the ZmARGOS genes. ZmARGOS is responsible for controlling plant growth, organ size and yield in crop plants.

Abstract: Provided are methods of increasing tolerance of a plant to abiotic stress, and/or increasing biomass, growth rate, vigor and/or yield of a plant. The methods are effected by expressing within the plant an exogenous polynucleotide encoding a polypeptide comprising an amino acid sequence at least 90% homologous to the amino acid sequence selected from the group consisting of SEQ ID NOs:201, 207, 212, 202-206, 208-211, 213-391, 1655, 961-1529, and 1660-1663. Also provided are polynucleotides, nucleic acid constructs, polypeptides and transgenic plants expressing same which can be used to increase tolerance of a plant to abiotic stress, and/or increase biomass, growth rate, vigor and/or yield of a plant.

Abstract: Embodiments of the present invention relate generally to drought tolerant transgenic plants and methods of creating the drought tolerant transgenic plants. In one embodiment, plants are transformed such that a PYL polypeptide is overexpressed in the plant. In an embodiment, the plant is transformed with a cassette or vector that comprises a polynucleotide encoding for one or more of the PYL polypeptides, which may be under the control of an inducible or constitutive promoter. In embodiments, overexpression of PYL13 results in plants having increased drought tolerance, such as a decreased transpiration rate, a decreased stomatal conductance, an increased photosynthetic rate, accelerated stress responsive gene expression, and increased water use efficiency, and/or an increased survival rate compared to a control plant. In some embodiments, other PYL proteins having the single point mutations identified in PYL13 are expressed in the plant.

Abstract: Inbred corn line designated BB202 is disclosed. The invention relates to the seeds of inbred corn line BB202, to the plants and plant parts of inbred corn line BB202 and to methods for producing a corn plant, either inbred or hybrid, by crossing inbred corn line BB202 with itself or another corn line. The invention also relates to products produced from the seeds, plants, or parts thereof, of inbred corn line BB202 and/or of the hybrids produced using the inbred as a parent. The invention further relates to methods for producing a corn plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other inbred corn lines derived from inbred corn line BB202.

Abstract: The present invention relates to plants of the Compositae family exhibiting a reversible genic male sterility trait, characterized in that the genic male sterility may be caused by a reduction or complete absence of endogenous jasmonic acid production, resulting from interference with one or more target genes involved in endogenous jasmonic acid production, selected from the group consisting of lipoxygenase, allene oxide synthase, allene oxide cyclase and 12-oxo-phytodienoic acid-10,11-reductase, or their functional homologs.

Abstract: A method of directing evolution of a target polynucleotide of interest for obtaining variants of this target polynucleotide, a method to generate genetic variability by preparing a cell library, and a method to isolate or to screen variants of a polynucleotide or variants of a protein able to impact the phenotype of a cell or to confer a desired phenotype to target cells, and to identify theses polynucleotide variants or protein variants responsible for this phenotype are described.

Abstract: The present invention provides novel, stable lipid particles having a non-lamellar structure and comprising one or more active agents or therapeutic agents, methods of making such lipid particles, and methods of delivering and/or administering such lipid particles. More particularly, the present invention provides stable nucleic acid-lipid particles (SNALP) that have a non-lamellar structure and that comprise a nucleic acid (such as one or more interfering RNA), methods of making the SNALP, and methods of delivering and/or administering the SNALP.

Abstract: The present disclosure generally relates to microorganisms that comprise one or more polynucleotides coding for enzymes in one or more pathways that catalyze a conversion of a fermentable carbon source to butadiene. Also provided are methods of using the microorganisms in industrial processes including, for use in the production of butadiene and products derived therefrom.

Abstract: A process for producing bioethanol includes the steps of pretreatment (consisting in destructuring the lignocellulosic vegetable raw material by placing it in the presence of a mixture containing formic acid, acetic acid and water, then in separating cellulose), of enzymatic hydrolysis and of alcoholic fermentation, characterized in that it includes, prior to the enzymatic hydrolysis, a step of partial elimination of the lignins so as to obtain a residual overall level of lignins (T), expressed as percentage by weight, which is non-zero and which is included in a range determined by a lower limit, and an upper limit Bsup, respectively equal to 0.30% and 4%. In order to obtain conditions of acidification before the enzymatic hydrolysis step, the process includes a step for re-acidification of the mixture, which is carried out with an acid, or of a mixture of acids, of determined pKa, and preferably with weak organic.

Abstract: The present invention provides a method for decreasing pyruvate catabolism and increasing the accumulation of pyruvate in microbes. By overexpressing wild type dihydrolipoamide acetyltransferase or dihydrolipoamide acetyltransferase mutants which have mutations at conservative active sites, the present invention provide a method to decrease overall activity of pyruvate dehydrogenase complex and pyruvate catabolism, and thus increase the accumulation of extracellular pyruvate without killing the pyruvate-producing microbes. Overexpressing dihydrolipoamide acetyltransferase mutants is an effective way to increase pyruvate accumulation.

Abstract: The invention refers to fungal cells, and especially to oleaginous fungal cells that have been genetically modified to produce enzymes of the pyruvate dehydrogenase bypass route to enhance their lipid production. Especially the cells are modified to over-express genes encoding pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD) and/or acetyl-CoA synthetase (ACS), optionally together with a gene encoding diacylglycerol acyltransferase (DAT), or to express genes encoding PDC together with ALD and/or ACS. Methods of producing lipids, biofuels and lubricants using the modified fungi are also disclosed as well as expression cassettes useful therein. A new enzyme having phospholipid: diacylglycerol acyltransferase (PDAT) activity and a polynucleotide encoding it are also disclosed, which are useful in the lipid production. A recombinant Cryptococcus cell and its construction is described.

Abstract: Genetically engineered microbial, e.g., Chlorella protothecoides, cells producing microbial oils are useful as a food additive and a source of renewable fuels and industrial chemicals. Lipid biosynthesis genes for Auxenochlorella protethecoides are disclosed that are useful for increasing lipid production and altering fatty acid and triacylglycerol profiles in recombinant microrganisms including cells of the genus Auxenochlorella or Prototheca.