Abstract: [Problem] To provide to a reagent composition used in quantitative measurement of lithium in aqueous solutions such as biological specimens and environmental liquid samples by a simple colorimeter or ultraviolet-visible light spectrophotometer immediately, a reagent composition which permits to measure a concentration of lithium by visual observation, and a method and apparatus for measuring lithium ion by using the reagent. [Solution] A reagent composition for measuring lithium comprising F28 tetraphenylporphyrin compound as chelating agent, including further a water-soluble organic solvent, a pH modifier and a stabilizer, and a method and apparatus for measuring lithium ion by using the reagent.

Abstract: The invention relates to enhancing translocation of a charged analyte through a transmembrane protein pore. Translocation is enhanced by increasing the net opposing charge of the barrel or channel and/or entrance of the pore. The invention also relates to pores enhanced in accordance with the invention.

Abstract: A composite, analyte sensor includes a substrate; a micro- or nano-electro-mechanical (MEMS; NEMS) resonator that is coupled to the substrate at least two edge locations (i.e., it is at least doubly-clamped) of the resonator, wherein the resonator is in a statically-buckled state near a buckling transition point of the resonator; and a chemically-responsive substance covering at least a portion of the surface of the resonator that will undergo a conformational change upon exposure to a given analyte. The resonator may be a double-clamped, statically-buckled beam (or bridge), a multiply-clamped, statically-buckled dome (or crater), or other resonator geometry. The sensor may include two or more at least double-clamped, statically-buckled, composite MEMS or NEMS resonators each operating near a buckling transition point of the respective resonator, and each characterized by a different resonant frequency. A method for sensing an analyte in ambient air.

Abstract: The monitoring breathalyzer has an alcohol sensor, a non-volatile memory, a processing unit or processor, and a screen. The processing unit determines the accuracy of the breathalyzer using the user's body as a simulator. In monitoring mode, the processing unit receives a BAC% measurement from the alcohol sensor based on the breath sample provided by the user at a sample time and determines a reference point from the BAC% measurement. The sample time is determined based on the user's metabolism rate or based on a time to a predetermined calibration point from a drink start time.

Abstract: The present invention describes a replaceable breath alcohol sensor module that can be replaced with a new pre-calibrated breath alcohol sensor module or re-calibrated. The breath alcohol sensor module requiring calibration can be removed from the body of a Breath Alcohol Testing Device (commonly called “breathalyzer” or “breathalyzer”). The breath alcohol sensor module can be separately calibrated for accurate calculation of “percent blood alcohol concentration” (% BAC) based on breath air samples and then re-installed into the breath alcohol tester or the old breath alcohol sensor module can be replaced with a new pre-calibrated breath alcohol sensor module. The present invention improves upon and/or replacing the current method of re-calibration at a breathalyzer service center.

Abstract: A screening method is described for selecting patient-specific anti-cancer agents reflecting individual genetic properties, in a precise and rapid manner, using an extremely small amount of cancer cells. Such screening method is useful for development of novel anti-cancer agents and the personalized medical field.

Abstract: Screening assays and methods of using same for screening to identify modulator agents or compounds that affect pcdh18 mediated inhibition of T cell effector function are described herein. Pharmaceutical and immunogenic compositions comprising agents or compounds that modulate pcdh18 mediated inhibition of T cell effector function are also encompassed. Methods for modulating pcdh18 mediated inhibition of T cell effector function using agents identified using assays described herein in pharmaceutical and immunogenic compositions are also envisioned. Adenocarcinoma is an exemplary tumor type that expresses pcdh18 and for which such pharmaceutical and immunogenic compositions would confer benefit to patients. Also encompassed are methods for reducing pcdh18 mediated inhibition of T cell effector function so as to achieve more effective T cell responses to pcdh18 expressing tumors.

Abstract: Novel lactoferrin fragments that are characteristic of inflammation during resolution and uses thereof. Diagnostic compositions and methods for assessing the presence or absence of resolving inflammation and for monitoring the progression of inflammatory resolution in a subject. Methods for treating a subject having an inflammatory disease, the methods including determining whether the subject has inflammation in resolution by determining the molecular weight of lactoferrin fragments.

Abstract: Methods for detecting in a sample the presence of an antibody to a conjugate of an antigen associated with a first carrier by a first association are disclosed. The method comprises contacting a conjugate of the antigen associated with a second carrier by a second association with said sample under conditions that allow binding of the antibody to the antigen; and introducing an agent to detect the presence of the antibody bound to said antigen. The first association and the second association are covalent associations and the first association is different from the second association. Also provided are kits, multiwell plates and conjugates that are useful in the method and further uses of the method. Also provided is a method of releasing a batch of a vaccine comprising a conjugate of an antigen associated with a first carrier by a first association and antibodies useful in this method.

Abstract: The invention provides assay particles useful, for example, for detecting analytes and binding molecule interactions. One type of assay particle includes a core portion encased by a shell portion, wherein the shell portion comprises an inorganic phosphor that binds selectively to a target molecule. Another type of an assay particle includes a core portion encased by a shell portion, and a coat portion covering the shell portion, wherein the coat portion comprises an inorganic phosphor that binds selectively to a target molecule. A further type of assay particle includes a core portion encased by a shell portion, and a coat portion covering the shell portion, wherein the coat portion comprises an inorganic phosphor and a target selective binding moiety, and wherein the assay particle is buoyant in aqueous media.

Abstract: The invention generally relates to conducting an assay on a sample that isolates a bacterium from the sample, in which the assay isolates as low as about 1 CFU/ml of bacteria in the sample.

Abstract: The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.

Abstract: Embodiments described herein include methods and assays for detecting beta-lactam analytes and mycotoxin analytes in a sample. Some embodiments include detection of multiple analytes in a sample utilizing a plurality of analyte binders and a control zone containing multiple control zone capture agents. In some embodiments, the multiple control zone capture agents capture a plurality of binders within one control zone. Test results are determined by comparison of the control zone signal to a test zone signal.

Abstract: A method of detecting and/or specifically identifying bacteria producing OXA-048-type carbapenemase in a biological sample, includes the steps of: (a) placing the biological sample likely to contain the bacteria in contact with a reaction medium including at least one chromogenic substrate to detect an enzymatic activity, and temocillin at a concentration equal to or greater than 150 mg/L, preferably between 200 and 500 mg/L, (b) incubating the sample in the medium to allow the bacteria to grow, and (c) detecting the strains corresponding to the OXA-48 carbapenemase producing bacteria. A culture medium as implemented in step (a) is also provided.

Abstract: Specific DNA sequences for binding various foodborne and waterborne pathogens and biotoxins are described. Each of these sequences can function in varying assay and sensor formats with varying degrees of success.

Abstract: The invention relates to the identification, sequencing, and isolation of an A25 bacteriophage lysin gene that expresses a protein involved in the lysis of bacterial cells during the phage life cycle. The invention further relates to methods for lysing certain bacteria using lysin, which are useful for example in a diagnostic procedure designed to detect these bacteria.

Abstract: Disclosed is a method that includes: (i) providing a plurality of initial nucleic acid cassettes that include: a) a first coding region encoding a first immunoglobulin variable domain, b) a second coding region encoding a second immunoglobulin variable domain, and c) a ribosomal binding site disposed between the first and second coding regions for translation of the second polypeptide in a first expression system, wherein the first and second coding regions are in the same translational orientation; (ii) modifying each nucleic acid cassette of the plurality in a single reaction mixture so that it is functional in a second expression system, wherein the first and second region remain physically attached during the modifying; (iii) introducing each modified nucleic acid cassette into a mammalian cell to produce a mixture of transfected cells; and (iv) expressing each modified nucleic acid cassette in the transfected cells.

Abstract: Provided herein is a large immuno-sorbent surface area assay (ALISSA) for the rapid and sensitive detection of botulinum neurotoxins (BoNTs) and anthrax toxin. This assay is designed to capture a low number of toxin molecules and to measure their intrinsic protease activity via conversion of a fluorogenic or luminescent substrate. Also provided herein are novel peptides that can be specifically cleaved by BoNT and novel peptides that are resistant to cleavage by BoNT. The combination of these cleavable and control peptides can be used for implementation of an exemplary ALISSA used to specifically detect BoNT enzymatic activity. Furthermore, the ALISSA as described herein may also be used in a column based format for use in a high-throughput system for testing large quantities of samples.

Abstract: Disclosed herein are methods and kits which are useful for detecting presence of an enzyme and the relative amount of glycan associated with the enzyme in a test sample based upon the enzyme's ability to competitively inhibit the binding of a ligand in such test sample. The present invention provides the ability to evaluate cell culture conditions and optimize the desired glycoform content of recombinantly prepared enzymes.

Abstract: Methods for the detection of, or the risk of, bladder cancer in a patient, comprising assaying for the presence of a combination of at least two biomarkers selected from CEA, VEGF, IL-8, NGAL, NSE, IL-2, EGF, TM, d-Dimer, MMP-9, IL-6, IL-4, MMP-9/NGAL, FAS, CRP, TUP and NMP22 in one or more samples isolated from a patient wherein detecting the presence of a combination of at least two biomarkers in the one or more samples indicates the presence or risk of bladder cancer.

Abstract: The invention is directed to non-invasive methods of detecting gastric cancer in an individual in need thereof comprising determining an expression level of inter-alpha-trypsin inhibitor heavy chain H3 (ITIH3) in the individual and comparing the expression level to a control, wherein an increase in the expression level of ITIH3 in the individual compared to the control is indicative of gastric cancer in the individual.

Abstract: Provided is aptamers screening method based on graphene without target immobilization and the aptamers obtained from the method, and more particularly, a new GO-SELEX method without target immobilization in which a single-stranded nucleic acid pool may react with a non-bound target material or a counter-target material, after which a single-stranded nucleic acid which has not been bound to the target or counter-target may be separated by using the graphene. Also, the specific aptamer obtained through the above-described method may be used for diagnosing target related diseases.

Abstract: Provided are an immunochromatography kit that is highly sensitive and capable of multicoloration, and organic colored microparticles that are ideal as an element of the immunochromatography kit. Organic colored microparticles having an average grain size between 10 and 1,000 nm and a color intensity between 1.0 and 5.0 are prepared using cellulose as the starting material. When the organic colored microparticles are used as a label in an immunochromatography kit, the immunochromatography kit is of a high sensitivity than conventional technology. The immunochromatography kit is also capable of multicoloration and is useful for rapid diagnosis.

Abstract: A sensor includes a substrate that includes an upper substrate and a lower substrate, and an electrode that is provided on the lower substrate, and the upper substrate, the lower substrate, and the electrode are formed by an optically transparent member.

Abstract: The present invention provides a method for acylating one or more amino groups of a peptide where the acylation reaction is to be performed in an aqueous mixture containing less than 10% w/w aprotic polar solvent.

Abstract: Compositions and methods for accurately characterizing immune reactivity to food allergens have been developed, in which test surfaces that incorporate different food antigen preparations derived from the same food into individual test sites are provided. Such coated surfaces can be produced using raw and cooked foods. The use of a panel of such test surfaces to characterize specific IgG, IgA, and/or C1q binding provides improved sensitivity and accuracy in determining immune reactivity and response to specific foods.

Abstract: Provided is a method for identifying and suppressing abnormal growth of fibroblasts at an early stage. Provided is a method for identifying the growth state of fibroblasts using as an index the level of expression of ETFB (electron transfer flavoprotein beta subunit), comprising: judging, in cases where the level of expression of ETFB is high, that there is a high probability that fibroblasts are abnormally growing; and judging, in cases where the level of expression of ETFB is low, that there is a high probability that fibroblasts are normally growing. Further, by inhibition of ETFB, abnormal growth of fibroblasts can be suppressed.

Abstract: Amount of combined nitric oxide or nitric oxide presented as iron nitrosyls in a blood sample is determined by directing a low power electromagnetic radiation beam at a blood sample to liberate nitric oxide gas, dissolving the liberated nitric oxide gas and electrochemically detecting amount of dissolved nitric oxide gas.

Abstract: In accord with one aspect, a microfluidic coagulation assessment device defining a plurality of microchannels is provided, wherein a blood sample is driven through the microchannels at a substantially constant flow rate and a controller is configured to, in combination with a timer and a pressure sensing device, determine a first pressure value (or flow value) at an initiation of flow, a first time (Tpg) at which a second pressure value is about twice the determined first pressure value, and a second time (Tpf) at which a third pressure value is about (1+e) times the determined first pressure value and establish a subject coagulation model predictive of channel occlusion therefrom. In another aspect, the blood sample is driven through the microchannels at a substantially constant pressure and a controller is configured to, in combination with a timer and a flow sensing device make the determination based on flow rate.

Abstract: A breath analyzer is described with at least one chemical sensor sensitive to the concentration of a component in a sample of exhaled breath including a compensator for compensating for the effect of variations in the amount of exhaled breath between the user and the sensor location with the chemical sensor being integrated into a portable electronic device, particularly with the sensor being located in an air duct with an opening to the exterior of the housing of the analyzer with the total area of the opening being sufficiently small to restrict effectively mass transport between the exterior and the sensor and/or wherein the compensation includes the compensation for different responses of sensors.

Abstract: A full-automatic detecting system and method for a transformer. The system comprises a material delivery line; a feed device; a detection delivery line; insulating test devices; error detecting devices; a laser marking device; a discharge device; and a detection management system. The insulating test devices, the error detecting devices, and the laser marking device are sequentially arranged beside the detection delivery line. The material delivery line, the detection delivery line, and the devices work in coordination, to realize full-automatic detection of the transformer, avoid manual detection errors and improve the sorting accuracy.

Abstract: Accuracy control of an automatic analysis device that mixes a sample and a reagent to measure temporal change of a mixed solution is realized. A plurality of measurement point data is acquired from a reaction process of the sample and the reagent. Parameters and test values of approximate equations for approximating the plurality of measurement point data are accumulated in a storage unit. A distribution map of reference data corresponding to the parameters or the test values is created based on predetermined numbers of the parameters or the test values accumulated in the storage unit. Next, a plurality of screens for individually superimposing, on the distribution map, curved lines corresponding to a plurality of regression function candidates obtained by applying a plurality of regression functions are arranged and presented on a display screen, so as to approximate the data to the distribution map of the reference data.

Abstract: A sample rack conveying unit 30 includes a sliding rail plate 53, a presser 66, a first guide plate 55, and a second guide plate 56. The sliding rail plate 53 has a groove portion 71 formed along a track on which a sample rack 90 slides and along which the sample rack 90 is conveyed. The presser 66 passes through the groove portion 71 and presses the sample rack 90. The first guide plate 55 is arranged on an outer side of a curved portion in a radial direction. The second guide plate 56 is arranged on an inner side of the curved portion in the radial direction.

Abstract: An apparatus for handling sample tubes is presented. The apparatus comprises a sample tube tray, a sample tube individualizer, a first conveyor, a second conveyor, a sample tube buffer, and a sample tube rack inserter. The sample tube tray stores sample tubes in bulk commodity. The sample tube individualizer sequentially unloads single sample tubes from the sample tube tray and sequentially provides the unloaded sample tubes to the first conveyor. The first conveyor conveys the sample tubes to the sample tube buffer. The sample tube buffer buffers the sample tubes provided by the first conveyor and provides buffered sample tubes to the second conveyor with a predeterminable, constant, sample tube rate. The second conveyor conveys the sample tubes to the sample tube rack inserter. The sample tube rack inserter inserts the sample tubes into a sample tube rack.

Abstract: Disclosed is an analyzer comprising: a liquid container mounting section in which a liquid container are set; a container mounting section in which at least one tip container accommodating a plurality of pipette tips is set; a cover detecting section that detects a presence of a cover mounted on the tip container; a dispensing section that equips a pipette tip accommodated in the tip container and dispenses a quantity of liquid from the liquid container to a reaction container via the equipped pipette tip; a detecting section that interrogate a property of the liquid; and a controller programmed to prohibit a process of equipping the pipette tip by the dispensing section when the cover on the tip container is detected, and permits the process when no cover is detected.

Abstract: An immunodiagnostic test element includes a support member, at least one test column containing a test material, and a wrap, such as an adhesively-attached foil wrap, covering the top of the at least one test element. The wrap includes a weakened portion directly above each test column, in which each weakened portion is formed by pre-stressing the portion, but not to the point of puncturing the wrap to enable the wrap to be punctured by a fluid aspirating/dispensing element, such as a metering tip, permitting access to the contents of the test column.

Abstract: A provision of a movement direction determination section for determining that a particular direction is a movement direction of the detection target object, in a case where a second comparator determines that larger than a predetermined threshold value is an absolute value of a ratio of (i) a difference between the photocurrent of the at least two light receiving elements to (ii) a sum of a photocurrent of at least two light receiving elements next to each other along the particular direction.

Abstract: A speed sensor for use in measuring the speed of rotation of a rotationally salient rotating member. The speed sensor comprises an electrode and a sensor circuit. The sensor circuit comprises a constant voltage source for supplying a voltage to the electrode to generate an electric field in a dielectric medium. A current detector detects current flow between the constant voltage source and the electrode due to perturbation of the electric field by passage of at least one salient feature of the rotating member through the electric field as the rotating member rotates. The current detector outputs a first signal modulated at a frequency corresponding to the frequency of perturbation of the electric field. The first signal is amplified to produce an amplified signal modulated at a frequency corresponding to the frequency of perturbation of the electric field.

Abstract: A system for detecting a vehicle speed, includes: a pulse ring including a pulse ring rotation part rotating according to travel of a vehicle, and a plurality of pitch units provided in the pulse ring rotation part at a predetermined interval; a vehicle speed sensor adjacently provided to the pulse ring and configured to output a voltage signal generated according to a distance between the vehicle speed sensor and a pitch unit; an output unit configured to output a voltage pulse signal and a voltage pulse amplitude change signal of the vehicle speed sensor; a first vehicle speed detection unit configured to detect the vehicle speed based on the voltage pulse signal of the output unit; and a second vehicle speed detection unit configured to detect the vehicle speed based on the voltage pulse amplitude change signal of the output unit, in which at least one of the plurality of pitch units has an adjacent distance which is different from an adjacent distance between another of the pitch units and the vehicle spe

Abstract: Techniques comprising obtaining, using a sensor unit coupled to a power line in a power distribution system, at least one measurement of at least one inertial property of the power line; and detecting at least one condition of the power line at least in part by analyzing the at least one measurement. A sensor unit configured to be coupled to a power line in a power distribution system, the sensor unit comprising an inertial sensor configured to collect at least one measurement of at least one inertial property of the power line.

Abstract: The invention provides an MEMS device. The MEMS device includes: a substrate, a proof mass, a spring, a spring anchor, a first electrode anchor, and a second electrode anchor, a first fixed electrode and a second fixed electrode. The proof mass is connected to the substrate through the spring and the spring anchor. The proof mass includes a hollow structure inside, and the spring anchor, the first electrode anchor, and the second electrode anchor are located in the hollow structure. The proof mass and the first fixed electrode form a first capacitor, and the proof mass and the second fixed electrode form a second capacitor. There is neither any portion of the proof mass nor any portion of any fixing electrode located between the first electrode anchor, second electrode anchor, and the spring anchor.

Abstract: With example embodiments described herein, a probe tip of a scanning probe microscope (such as an atomic force microscope (AFM)) is directly detected as it moves in a tapping mode to determine the tip positions over time, and a force for the tip is computed from these determined tip positions.

Abstract: An active probe pod used in a logic analyzer is disclosed. The active probe pod may be connected to the logic analyzer having a FPGA decoder and to a DUT circuit board. The active probe pod may include a LVDS differential ire component connected to the FPGA decoder and a front-end circuit board for capturing a weak signal input from the DUT circuit board. The front-end circuit board is adapted not to transmit the captured weak signal input over a long-distance signal transmission path, which helps minimize interferences with the weak signal input, while outputting a LVDS differential signal to the FPGA decoder for decoding. As the front-end circuit board is used for capturing the weak signal input, which falls within the category of one short-distance signal transmission, the signal reflection may not take place, without affecting the signal quality and/or attenuating the signal strength.

Abstract: A measurement device includes a stage for carrying an object to be measured, an insulating board having a through hole, a probe fixed on the undersurface of the insulating board, a side wall section in a shape surrounding the probe, a pressurizing section provided on the top surface of the insulating board, the pressuring section supplying a gas below the insulating board via the through hole, and a measurement section electrically connected to the probe to control the pressurizing section, wherein the measurement section measures an electric property of the object to be measured via the probe in a state where the pressurizing section is controlled to supply a gas to a measurement space located below the insulating board to increase a pressure in the measurement space, the measurement space surrounded by the stage, the side wall section, and the insulating board.

Abstract: A method for the contactless determination of an electrical potential of an object, involves providing an electrode which is spatially at a distance from the object, connecting the electrode to a reference potential, determining a first temporal change in an electrical state of charge of the electrode at a first value for the electric flux between the electrode and the object, determining a second temporal change in the electrical state of charge of the electrode at a second value for the electric flux between the electrode and the object, and determining the electrical potential of the object at least from the first temporal change in the electrical state of charge and the second temporal change in the electrical state of charge and from a difference between the first value and the second value for the electric flux.

Abstract: Devices and methods for sensing current are described herein. One device (100) includes a base member (102) having a first leg (104, 106) and a second leg (104, 106), the legs (104, 106) defining an angle (108) therebetween, a first magnetic current sensor (110, 112) coupled to the base member (102) and positioned at a first location in a plane bisecting the angle (108), and a second magnetic current sensor (110, 112) coupled to the base member (102) and positioned at a second location in the plane bisecting the angle (108).

Abstract: A current sensor includes a folded-shaped current path including a pair of arm portions extending in parallel with each other, and a pair of magnetoelectric conversion elements provided so as to sandwich therebetween a symmetric axis passing between the pair of arm portions, the pair of magnetoelectric conversion elements being used for detecting magnetism caused by a current passing through the pair of arm portions, wherein a half-bridge circuit in which the pair of magnetoelectric conversion elements is series-connected and a signal is able to be extracted from a connection point between the pair of magnetoelectric conversion elements is formed, and sensitivity axes of the pair of magnetoelectric conversion elements are oriented in a same direction and sensitivity-influencing axes of the pair of magnetoelectric conversion elements are oriented in a same direction.

Abstract: Methods and systems are described for diagnosing a multiple speed, relay controlled engine cooling fan system. One method includes measuring the change in current drawn from a battery-alternator system when fan speed is modified. A degradation in cooling fan operation is flagged when measured current change differs from an expected change.

Abstract: A technique for determining performance characteristics of electronic devices and systems is disclosed. In one embodiment, the technique is realized by measuring a first response on a first transmission line from a single pulse transmitted on the first transmission line, and then measuring a second response on the first transmission line from a single pulse transmitted on at least one second transmission line, wherein the at least one second transmission line is substantially adjacent to the first transmission line. The worst case bit sequences for transmission on the first transmission line and the at least one second transmission line are then determined based upon the first response and the second response for determining performance characteristics associated with the first transmission line.

Abstract: In at least one embodiment, an apparatus for detecting a phase sequence in a vehicle is provided. The apparatus includes a control module for being operatively coupled to a power grid for receiving an incoming power signal therefrom, the incoming power signal providing a first voltage and a second voltage in a vehicle. The control module is configured to determine a phase difference between the first voltage and the second voltage and to determine a signal frequency of the incoming signal to determine a country type for the power grid. The control module is further configured to control a number of switching devices to generate an output voltage signal based on the phase difference and the signal frequency to charge at least one battery on the vehicle.