Abstract: A detergent emulsifier for laundry and other hard surface cleaning using an APE-free surfactant blend is disclosed. The emulsifier system is efficacious for removal of oily soils and greasy food removal. The compositions according to the invention include linear and branched fatty alcohol ethoxylates and ethoxylate propoxylate block copolymers. Methods of using the same are disclosed.

Abstract: The present invention relates to novel cleaning compositions that are substantially free of cocamide diethanolamine. In an aspect of the invention, the compositions utilize a surfactant system and coupling agents as a replacement for the rheology modifier cocamide diethanolamine. The combination of a surfactant system and coupling agents can be used as a replacement for traditional rheology modifiers and foaming agents which are under regulatory pressure while providing concentrated liquid cleaning compositions with a viscosity of 400 cps or less. In another aspect the invention relates to novel cleaning compositions such as pot and pan soaking compositions, dishwashing compositions, food and beverage foaming cleaners, vehicle cleaning and the like suitable for use in hard water, which can be solid or liquid. The invention further relates to methods of making these compositions, and to methods employing these compositions.

Abstract: The need for a stable, compact composition providing improved fabric care benefit, that is also convenient to use, can be met by incorporating a cationic cellulose polymer into a non-aqueous composition, using a non-aqueous dispersant. Such compositions have good physical stability, with little or no clumping of the cationic polymer in particulate form.

Abstract: The invention relates to cleaning and/or treatment compositions comprising tannins and methods of making and using same. Such cleaning and/or treatment compositions can mitigate/neutralize malodors without imparting color to an article that is treated with such cleaning and/or treatment composite. In addition, such the appearance of such compositions is not adversely impacted by the tannins that they contain.

Abstract: The invention relates to a concentrated detergent composition comprising an alkali metal carbonate, methylglycinediacetic acid, glutamic acid N,N-diacetic acid, and alkali metal tripolyphosphate. The composition is particularly suited to remove tea and coffee soil in warewashing applications.

Abstract: This disclosure relates to detergent compositions containing pyridinol-N-oxide compounds and being substantially free of bleach. Methods for treating a stained fabric using such detergent compositions are also disclosed.

Abstract: The present invention relates, in part, to cleaning methods and solvent cleaning compositions including at least one hydrofluoro-olefin or hydrochlorofluoro-olefin solvent for use in connection with cleaning of metal parts, and in certain preferred embodiments cleaning metal parts to be used in an aircraft.

Abstract: A device for a precipitating solid material particles from a suspension of a brew liquid including dissolved CO2 and plant based aroma hearer solid material particles, the device including a filter surface which separates a non-filtrate space from a filtrate space wherein the non-filtrate space receives the suspension; a suspension inlet leading into the non-filtrate space, a filtrate outlet opening into the filtrate space; a mechanical compressor that is provided in the non-filtrate space and configured to feed the suspension under a separation of the brew liquid through the filter surface within the non-filtrate space along the filter surface to a compression portion of the non-filtrate space and to compress the remaining solid materials: and a solid material output opening provided in a wall of the non-filtrate space in the compression portion of the non-filtrate space wherein the solid material output opening is closeable tight relative to an ambient atmosphere.

Abstract: An anaerobic digestion system is provided that includes a blend tank operable to control and perform pre-treatment of feedstock. An anaerobic digester is operable to digest the feedstock provided from the blend tank in a totally enclosed oxygen-free environment within a specific temperature range. A bio-mass tank processes liquid digestate from the anaerobic digester. One or more baffles are positioned in the digester, with the one or more baffles providing for plug flow through at least a portion of the digester to create baffled zones that are at least partially operable independently of adjacent baffled zones. A bio-mass tank processes liquid digestate from the anaerobic digester. An energy source is coupled to the anaerobic digester.

Abstract: An apparatus 1 for producing a cell mass sheet is structured so as to produce a cell mass sheet 4 in which a plurality of cell masses 2 that have been planarly arranged on a mounting surface 13a in a culture container 3 are fused to each other by being cultured. The apparatus includes guide means G that has a plurality of accommodating portions Ga which can accommodate the cell masses 2 therein, and suction nozzles 8 (supply means) that supply the cell masses 2 into the accommodating portions Ga of the guide means G, which are arranged on the mounting surface 13a; and is structured so that the suction nozzles 8 array the cell masses 2 on the mounting surface 13a, and after that, the guide means G retracts from the above the mounting surface 13a. The apparatus can produce a cell mass sheet that has a constant shape and also a uniform thickness.

Abstract: A bag assembly for use with a heat exchanger includes a flexible bag having of one or more sheets of polymeric material, the bag having a first end that bounds a first compartment and an opposing second end that bounds a second compartment, a support structure being disposed between the first compartment and the second compartment so that the first compartment is separated and isolated from the second compartment. A first inlet port, a first outlet port, and a first drain port are coupled with the flexible bag so as to communicate with the first compartment. A second inlet port, a second outlet port, and a second drain port are coupled with the flexible bag so as to communicate with the second compartment.

Abstract: A reactor system includes a support housing having an interior surface bounding a chamber, the chamber having a vertically extending central longitudinal axis. A flexible bag is disposed within the chamber of the support housing and has an interior surface bounding a compartment. A mixing element is disposed within the compartment of the flexible bag. A drive element, such as a drive shaft, is secured the mixing element, wherein the mixing element is laterally offset from and/or is angled relative to the vertically extending central longitudinal axis of the support housing.

Abstract: Devices and methods cell culture are disclosed herein. In particular embodiments, the cell culture devices include multi-channel devices with a non-linear flow path that recapitulates the three-dimensional microarchitecture and physiological organ-level functions of human organs with cellular and molecular resolution.

Abstract: An algae production reactor system according to the invention comprises a reactor vessel which is provided with:—one or more liquid inlets and one or more liquid outlets;—one or more gas inlets at the bottom, said gas inlets being connected with a source of carbon dioxide, and one or more gas outlets at the top of the vessel;—vertically interspaced and joined pairs of double glass plates which are at least partially submerged in the reactor liquid, said double glass plates having a layer of light-scattering particles in between and having a flat side being exposed to a light source; and—means for vertically circulating reactor liquid.

Abstract: An acoustophoresis device made up of modular components is disclosed. Several modules are disclosed herein, including ultrasonic transducer modules, input/output modules, collection well modules, and various connector modules. These permit different systems to be constructed that have appropriate fluid dynamics for separation of particles, such as biological cells, from a fluid.

Abstract: A bioreactor is provided. The bioreactor is a multi-scalable bioreactor, which comprises a culture vessel for seeding and culturing cells by adding a cell-culture media, wherein the culture vessel comprises at least a side wall and a bottom surface, a specific heat transfer area and a specific gas transfer area; wherein the culture vessel is configured to accommodate the cell-culture media volume upto 10 liters, and wherein the specific heat transfer area and the specific gas transfer area are independent of cell-culture media volume. A kit for culturing cells in a large scale is also provided which further comprises disposable tubings, culture bag or combinations thereof. A method for culturing cells is also provided.

Abstract: A harvesting device and methods of harvesting (i.e., dewatering) algae are described. The harvesting device and methods involve the use of stimuli-sensitive hydrogels.

Abstract: The invention provides methods and compositions for increasing photosynthetic efficiency and biomass production in cyanobacterial cultures by minimizing the phycobilisome light-harvesting antenna size through disruption of the phycocyanin-encoding CPC-operon.

Abstract: The present invention concerns the separation, identification and characterization of active peptide fragments from peptones.

Abstract: A physical self-organizing hydrogel system for biotechnological applications composed of a non-covalent network on the basis of a protein-ligand interaction includes a tetrameric protein and biotin or a derivative thereof as a ligand, wherein biotin or derivative is covalently conjugated to an end of a polymer chain or a linear or multi-arm synthetic polymer of a single-or double-strand oligonucleotide and wherein the solid content in relation to the entire hydrogel is at least 3% and the conjugates are crosslinked by the tetrameric protein. A mixture ratio is a molar equivalent ratio between the protein and the number of terminal biotinylated ends of the polymer chains in the biotin-polymer conjugate of 1:2 to 1:8. The hydrogel formation occurs with controlled kinetics on the basis of protein-ligand interaction.

Abstract: The present disclosure relates to fabricating sacrificial microfiber templates from any biocompatible and resorbable materials depending on the time needed for dissolving the microfiber template to free the endothelial tube with open lumen. Microfiber networks with distinct patterns and defined diameters initially serve as a template to support the growth of vascular cells (endothelial cells or their progenitor cells, or combined with mural cells such as pericytes) and then dissolve to form an empty endothelium lumen. The incorporation of sacrificial microfiber networks encapsulated with vascular cells into 3D cell-rich constructs allows for the creation of various vascularized tissues.

Abstract: The present invention relates to a method of isolating a pluripotent cell from a pre-implantation embryo including one or more pluripotent cells. The method includes propagating the one or more pluripotent cells from the embryo under conditions that allow undifferentiated growth of the one or more pluripotent cells and do not allow growth of non-pluripotent cells from the embryo.

Abstract: The composition for producing neural stem cells provided by the present invention contains an artificially synthesized synthetic peptide having a neural stem cell-inducing peptide sequence that induces neural stem cells from fibroblasts, and one or two or more pharmaceutically acceptable carriers. The neural stem cell-inducing peptide sequence is any of (i) an amino acid sequence constituting a signal peptide of any protein belonging to the amyloid precursor protein (APP) family, (ii) a partial amino acid sequence of the amino acid sequence of (i), and (iii) a modified amino acid sequence of the amino acid sequence of (i) or (ii).

Abstract: Methods and compositions relating to the production of induced pluripotent stem cells (iPS cells) are disclosed. For example, induced pluripotent stem cells may be generated from peripheral blood cells, such as human blood progenitor cells, using episomal reprogramming and feeder-free or xeno-free conditions. In certain embodiments, the invention provides novel methods for improving overall reprogramming efficiency with low number of blood progenitor cells.

Abstract: The invention relates to polynucleotides suitable for reducing or eliminating the expression of expanded repeat RNA (CUGexp) of the dystrophy myotonic-protein kinase (DMPK) gene in a cell of a DM-1 patient. The polynucleotides are a combination of a polynucleotide for a site specific nuclease targeting the dystrophy myotonic-protein kinase (DMPK) gene locus, and a donor polynucleotide having 5? and 3? regions which are homologous with the sequence of DMPK gene which flank the target site of the nuclease. The invention further relate to in vivo and in vitro methods to reduce or eliminate CTG repeats in the DMPK gene. The invention further relates to the medical use of polynucleotides and cells for treating DM-1 patient.

Abstract: The present invention relates to a method of generating an induced pluripotent stem (iPS) cell comprising the step of introducing into a target cell one or two coding sequences each giving rise upon transcription to a factor that contributes to the reprogramming of said target cell into an induced pluripotent stem cell and selected from Oct3/4 or a factor belonging to the Myc, Klf and Sox families of factors, wherein the target cell endogenously expresses at least the factors that are not encoded by the coding sequences to be introduced and selected from Oct3/4 or factors belonging to the Myc, Klf and Sox families of factors, and wherein the cell resulting from the introduction of the one or two coding sequences expresses the combination of factor Oct3/4 and at least one factor of each family of factors selected from the group of Myc, Klf and Sox.

Abstract: Disclosed herein are methods and compositions for calicivirus. Methods comprise in vitro cell culture systems used for diagnosis, identification, attenuation and other uses. Compositions comprise in vitro cell culture systems for calicivirus.

Abstract: The present invention relates to drug targets for Burkholderia pseudomallei. The invention provides a crystalline polypeptide derived from Burkholderia pseudomallei comprising the amino acid sequence set forth in SEQ ID NO: 1. Also provided are methods for co-crystallizing a binary enoyl-acyl carrier protein reductase (FabI) with a potential inhibitor of an FabI activity and for identifying an inhibitor of an activity of enoyl-acyl carrier protein reductase (FabI). A representative example of such a crystalline structure is a BpmFabI:AFN-1252 complex.

Abstract: [Problems] To provide a glucose dehydrogenase, a polynucleotide encoding the enzyme, a method for manufacturing the enzyme, a method for measuring glucose using the enzyme, a measuring reagent composition, and a biosensor. [Solutions] A flavin-conjugated glucose dehydrogenase which is composed of proteins having the following amino acid sequence (a), (b) or (c), and having glucose dehydrogenase activity: (a) an amino acid sequence represented by SEQ ID NO: 2, 3, 5, 6, 8 or 9; (b) an amino acid sequence in which one or more amino acids are deleted from, replaced in or added to the amino acid sequence represented by SEQ ID NO: 2, 3, 5, 6, 8 or 9; (c) an amino acid sequence having at least 85% identity with the amino acid sequence represented by SEQ ID NO 2 or 3, at least 95% identity with the amino acid sequence represented by SEQ ID NO 5 or 6, or at least 80% identity with the amino acid sequence represented by SEQ ID NO 8 or 9.

Abstract: The object of the present invention is to efficiently produce useful terpenoid compounds, and specifically, to provide a method for preparing squalene, which is an important intermediate of terpenoid. The object can be solved by a hydroxymethylglutaryl CoA reductase (HMGR) comprising:(a) an amino acid other than alanine (A) at the 10th position in an S?2 amino acid sequence of HMGR, (b) an amino acid other than proline (P) at the 1st position from the carboxyl terminal in the DKK region of the HMG-CoA binding site of HMGR, (c) an amino acid other than alanine (A) at the 1st position in an L?2 amino acid sequence of HMGR, and (d) an amino acid other than glutamic acid (E) at the 6th position in an L?2 amino acid sequence of HMGR of the present invention.

Abstract: Disclosed herein are methods and compositions for targeted modification of one or more endogenous plant malate dehydrogenase genes.

Abstract: The present invention relates to novel NADPH oxidase, or Nox, proteins, to the use thereof, to a method for preparing same and to a method for identifying same.

Abstract: The present invention relates to a mutant CC3625 cysteine synthase. Bacteria containing such mutant cysteine synthase can be used for the precipitation of soluble lead.

Abstract: Methods for treating latent viral infections using a gene for a nuclease that is expressed in the presence of a latent viral infection, allowing the nuclease to digest viral nucleic acid. The gene is controlled by a switch that turns expression on in the presence of viral transcripts. The switch may be an engineered sequence that, in the absence of a viral transcript, forms a duplex structure to inhibit translation. The viral transcript hybridizes to the switch and disrupts the duplex structure, allowing translation to occur. A nucleic acid encodes a nuclease and a switch that causes the nuclease to be expressed in the presence of a viral nucleic acid. A portion of the switch may be complementary to at least a portion of a latency associated transcript such as an HHV latency associated transcript that, when present, interacts with the switch to initiate translation of the nuclease.

Abstract: A method for one of altering and enhancing the stereospecificity of an enzyme comprising introducing a stereospecific editing domain into the enzyme.

Abstract: Nucleic acid-guided ordered protein assembly (NOPA) arrays and methods for their generation and related applications are disclosed herein.

Abstract: Provided are nucleic acid extraction apparatuses and operation methods thereof. The apparatus may include at least one cyclically moveable annular structure, at least one pipetting mechanism, at least one injection mechanism and a driving mechanism. The annular structure may be provided with a plurality of cuvette positions and a plurality of operation positions. The pipetting mechanism and the injection operation may be arranged along the annular structure. The driving mechanism may drive the annular structure to move cyclically.

Abstract: The present invention provides synthetic nucleic acid molecule tags that can be added into samples for identification and tracking. Among other things, the present invention provides synthetic, high-molecular weight concatemers, which can be combined with samples to yield hundreds of millions of unique identifiers. Examples applications for which the synthetic nucleic acid molecule tags can be used, include industrial, research and clinical applications.

Abstract: Sensitive, unbiased methods for genome-wide detection of potential CRISPR-Cas9 off-target cleavage sites from cell type-specific genomic DNA samples.

Abstract: Certain embodiments are directed to methods of identifying neuroblastoma differentiation-inducing compounds or agent and their use in treating neuroblastoma.

Abstract: Disclosed herein are compounds, compositions and methods for modulating splicing of SMN2 mRNA in a cell, tissue or animal. Also provided are uses of disclosed compounds and compositions in the manufacture of a medicament for treatment of diseases and disorders, including spinal muscular atrophy.

Abstract: The invention relates to compositions and methods utilising miR-7 microRNA

Abstract: Disclosed herein are methods of treating and diagnosing muscular dystrophy. In some examples, the methods include treating muscular dystrophy by administering to the subject a therapeutically effective amount of an agent that alters the expression of at least one miR gene product, such as miRNA-124 and/or miRNA-29 thereby treating muscular dystrophy. In one particular example, the method of treatment includes administering an agent that decreases the expression or activity of miRNA-124. In another embodiment, the method of treatment includes administering a composition that includes one or more agents to decrease the expression and/or activity of miRNA-124 and one or more agents to alter the activity of miRNA-29 (increase or decrease). Also disclosed are methods of enhancing muscle regeneration, repair, or maintenance in a subject and methods of enhancing ?7?1 integrin expression. Methods of prospectively preventing or reducing muscle injury or damage in a subject are also disclosed.

Abstract: This application provides and discloses anti-parasitic, anti-pest or insecticidal nucleic acid molecules and their calmodulin target genes for the control of arthropod parasites and pests. This application further provides methods and compositions for the control and treatment of parasites and pests in Apis mellifera (honey bee) hives.

Abstract: Materials and methods related to using multimeric DNA aptamers to treat demyelinating diseases are provided herein.

Abstract: The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or ?-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB).

Abstract: The present invention is directed to isolated nucleic acid molecules and polypeptides of thraustochytrid polyunsaturated fatty acid (PUFA) synthases involved in the production of PUFAs, including PUFAs enriched in docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), or a combination thereof. The present invention is directed to vectors and host cells comprising the nucleic acid molecules, polypeptides encoded by the nucleic acid molecules, compositions comprising the nucleic acid molecules or polypeptides, and methods of making and uses thereof.

Abstract: The invention refers to fungal cells, and especially to oleaginous fungal cells that have been genetically modified to produce enzymes of the pyruvate dehydrogenase bypass route to enhance their lipid production. Especially the cells are modified to overexpress genes encoding pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD) and/or acetyl-CoA synthetase (ACS), optionally together with a gene encoding diacylglycerol acyltransferase (DAT), or to express genes encoding PDC together with ALD and/or ACS. Methods of producing lipids, biofuels and lubricants using the modified fungi are also disclosed as well as expression cassettes useful therein. A new enzyme having phosholipid:diacylglycerol acyltransferase (PDAT) activity and a polynucleotide encoding it are also disclosed, which are useful in the lipid production. A recombinant Cryptococcus cell and its construction is described.

Abstract: The present invention relates to a novel enhancer of protein production in host cells. It discloses a vector for expressing recombinant proteins in these cells, comprising a nucleotide sequence encoding a) a secretion peptidic signal, b) a 6-methylguanine-DNA-methyltransferase enzyme (MGMT, EC 2.1.1.63), a mutant or a catalytic domain thereof, and c) a recombinant protein. Said MGMT enzyme is preferably the so-called SNAP protein.

Abstract: The present invention relates to the production and use of covalently closed circular (ccc) recombinant plasmids, and more particularly to vector modifications that improve expression of said DNA molecules in the target organism.