Abstract: Panels, compositions, and methods for treating cancer in a subject in need thereof are disclosed involving one or more genes the suppression of which renders the cancer chemosensitive and/or radiosensitive.

Abstract: The invention provides non-natural microbial organisms containing enzymatic pathways and/or metabolic modifications for enhancing carbon flux through acetyl-CoA, or oxaloacetate and acetyl-CoA. Embodiments of the invention include microbial organisms having a pathway to acetyl-CoA and oxaloacetate that includes phosphoketolase (a PK pathway). The organisms also have either (i) a genetic modification that enhances the activity of the non-phosphotransferase system (non-PTS) for sugar uptake, and/or (ii) a genetic modification(s) to the organism's electron transport chain (ETC) that enhances efficiency of ATP production, that enhances availability of reducing equivalents or both. The microbial organisms can optionally include (iii) a genetic modification that maintains, attenuates, or eliminates the activity of a phosphotransferase system (PTS) for sugar uptake.

Abstract: A fusion protein based on the heavy chain of human ferritin is described, which includes at the N terminus of the protein at least one metalloproteinase cleavage sequence and a PAS polypeptide that acts as a masking polymer that increases the protein-drug stability, as well as a nanoparticle composed of multiple monomers of said fusion protein, a nucleic acid encoding for said fusion protein, and diagnostic and therapeutic applications thereof.

Abstract: The invention relates to a polynucleotide encoding a polypeptide based on the minimum region of the Orthoreovirus muNS protein that can form inclusions in the endoplasmic reticulum, and to said polypeptide. The invention also relates to a purification method and a method for detecting interaction between two polypeptides based on the capacity of some regions of the Orthoreovirus muNS protein to incorporate themselves into the inclusions, together with a peptide of interest.

Abstract: The present disclosure relates to an expression cassette including an rrnA promoter derived from Vibrio natriegens for enhancing the growth rate of Escherichia coli and recombinant Escherichia coli, into which the expression cassette is introduced. More particularly, the present disclosure relates to an expression cassette including the Vibrio natriegens-derived rrnA promoter for enhancing the growth rate of Escherichia coli by introduction thereof into an rrn operon promoter region of Escherichia coli.

Abstract: A method for the expression of a protein of interest by a bacterium, notable in that it comprises the culturing of a bacterium temporarily or continuously expressing an Hsp protein, in that said bacterium also comprises a nucleic acid sequence, encoding a protein of interest, under the control of a lac promoter and in that said bacterium is cultured in a medium which does not contain IPTG or a metabolized molecule in such a way as to automatically induce the induction of transcription from the lac promoter.

Abstract: The invention relates to a nucleic acid molecule encoding a novel selection marker. Said marker is a guanidinobutyrase from Kluyveromyces lactis, which, when expressed in Saccharomyces, allows the growth of the yeast in the presence of guanidinobutyrate as the sole nitrogen source. Said marker can be used in a method for producing a microorganism having an altered genome. The invention further relates to a set of constructs, comprising a first construct comprising a recognition site for an endonuclease, a first region of homology with a target gene of a microorganism, and a first part of a nucleotide sequence encoding the selection marker, and a second construct comprising a second part of the nucleotide sequence encoding the selection marker, a second region of homology with the target gene of the microorganism, and a copy of the endonuclease recognition site.

Abstract: This disclosure features fusion proteins comprising a base protein linked to or incorporated in a CH2 scaffold of IgG. The CH2 scaffold can derive from the macaque CH2 domain of IgG. The fusion proteins can effectively bind a single or multiple targets, and can be engineered to regulate effector functions as desired. The fusion proteins can have an increased serum half-life, solubility, stability, protease resistance, and/or expression as compared to the scaffolds alone and/or as compared to the base protein alone. This disclosure also features fusion proteins comprising a base protein, a CH2 scaffold and a discrete polyethylene glycol (dPEG) linked to the scaffold via a serine, tyrosine, cysteine, lysine, or a glycosylation site of the scaffold. This disclosure additionally features scaffolds linked to a discrete polyethylene glycol (dPEG) via a serine, tyrosine, cysteine, or lysine of the scaffolds or a glycosylation site of the scaffold.

Abstract: Disclosed herein are viral vectors suitable for transfection into woody trees for purposes of delivering and expressing beneficial genes. Specifically exemplified herein are vectors for transfecting citrus trees. The vectors allow for the expression of useful proteins, such as those that can protect the tree from disease. Specifically exemplified herein are methods of transfecting woody trees that allow multiple applications of vectors while avoiding superinfection exclusion.

Abstract: There are provided compositions and methods for transforming plants, preferably monocot, and even more preferably, sugarcane. The transformation methods involve infection of plant tissue with Agrobacterium, and co-cultivation using culture medium comprising high concentrations of gelling agent, with the result of inhibiting the exacerbated growth of the bacteria and increasing the transformation frequencies. The invention includes regenerating transformed plants, and the transformed plants themselves.

Abstract: The disclosure relates to gene expression regulatory sequences from soybean, specifically to the promoter of a soybean eukaryotic translation initiation factor 5A-2-likegene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in a constitutive manner in plants. The disclosure further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a Zea mays GRMZM2G138258 promoter. Some embodiments relate to a Zea mays GRMZM2G138258 promoter that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a Zea mays GRMZM2G138258 3?UTR that functions in plants to terminate transcription of operably linked nucleotide sequences.

Abstract: Compositions and method of delivering a molecule to a plant are provided. In an embodiment, an avenic acid transporter is introduced into a plant. The plant may be a non-graminacious or dicotyledonous plant which does not comprise the transporter in the wild type form. The transporter may be modified to increase uptake of avenic acid along with iron chelated by the avenic acid and/or a molecule conjugated with the avenic acid. Further embodiments provide for conjugating the avenic acid with a molecule for uptake and delivery to the plant. In this manner plant health may be improved by uptake of iron where it would otherwise not occur and/or uptake of the conjugated molecule. The molecule may be a molecule that improves health of the plant. Still further embodiments provide for analogs of avenic acid. Embodiments provide for interplanting Avena sativa which natively produces avenic acid with another plant. Additional embodiments provide for time release of avenic acid provided to a plant.

Abstract: The invention relates to nucleic acids, proteins and methods for modulating the cellulose content of plants.

Abstract: This disclosure provides tobacco plants that exhibit altered photosynthesis as well as methods of making and using such plants.

Abstract: This invention relates to the identification of a regulator protein (termed CYP78A6, or EOD3) which controls the size of plant seeds and organs in Arabidopsis and other plants. Manipulation of CYP78A protein expression may useful, for example, in improving crop yield and increasing plant biomass.

Abstract: It is intended to provide a gene capable of conferring more practical environmental stress tolerances to a plant and the use thereof. The present invention provides a plant having enhancement in one or two or more genes selected from the group consisting of a second gene selected from a gene group consisting of a first gene subgroup consisting of Os12g0150200 gene, Os01g0858350 gene, Os05g0445100 gene, Os11g0151400 gene, AT3G48520 gene, and AT2G27690 gene, and a gene functionally equivalent to any of these genes and a second gene subgroup consisting of Os04g0584800 gene and a gene functionally equivalent to this gene.

Abstract: This disclosure relates to novel mutant alleles for increasing biomass in plants and provides mutant plants comprising alleles and chimeric genes encoding the novel alleles.

Abstract: The present disclosure provides borage plants having increased resistance to imidazolinone herbicides. More particularly, provided herein are methods for generating herbicide resistant borage and testing of selected progeny for homozygosity. Nucleic acids encoding AHAS 1 and 2 genes that encode herbicide resistance in borage are provided.

Abstract: The subject invention includes methods and plants for controlling lepidopteran pests, especially soybean looper (Pseudoplusia includens) and velvet bean caterpillar (Anticarsia gemmatalis) insects. The plants, preferably soybean plants, comprise a combination of Cry1Ca, Cry1Ea, Cry2Aa, and Vip3Ab1 insecticidal proteins. Methods are described for using the plants to delay or prevent the development of resistance by insects.

Abstract: Genetic male sterile plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants and methods of use of said plants, are provided, including use of parental plants to produce sterile plants for hybrid seed production.

Abstract: Compositions and methods are capable of modulating male fertility in a plant. Compositions comprise polynucleotides and polypeptides, and fragments and variants thereof, which modulate male fertility. Expression cassettes comprise a male-fertility polynucleotide, or fragment or variant thereof, operably linked to a promoter, wherein expression of the polynucleotide modulates the male fertility of a plant. The level and/or activity of a polynucleotide that influences male fertility is modulated in a plant or plant part. Regulatory sequences drive expression in a male-tissue-preferred manner and may be targets to downregulate an operably linked gene. Methods to track mutations that induce nuclear recessive male sterility in subsequent selfing and crossing of wheat lines containing the mutations are also provided. Male-sterile plants may be maintained by pollinating with a maintainer plant.

Abstract: The invention provides a method for generating a transgenic eukaryotic cell population having a modified human Rosa26 locus, which method includes introducing a functional DNA sequence into the human Rosa26 locus of starting eukaryotic cells. Also provided are targeting vectors useful in the method, as well as a cell population and a transgenic non-human animal comprising a modified human Rosa26 locus. Finally, the invention provides an isolated DNA sequence corresponding to the human Rosa26 locus.

Abstract: Disclosed herein are methods and compositions for targeted deletion of double-stranded DNA. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain, and polynucleotides encoding same. Methods for targeted deletion include introduction of such fusion proteins, or polynucleotides encoding same, into a cell such that two targeted cleavage events occur. Subsequent cellular repair mechanisms result in deletion of sequences between the two cleavage sites.

Abstract: The present invention provides a recombinant turkey herpesvirus modified by the presence of the cDNA encoding the hemagglutinin protein of avian influenza virus under a promoter. A poultry vaccine comprising the recombinant turkey herpesvirus described in the present invention can induce serological responses that may be easily detected by the hemagglutination inhibition assay but not by commercially available diagnostic ELISA kits; thus enabling easy differentiation between vaccination and field infection.

Abstract: Expression vectors ideal for use in vaccinating individuals against disease based on vaccinia virus and other chordopoxviruses having high expression of recombinant genes and low expression of vector genes in target animals, and low expression of recombinant genes and high expression of vector genes in cells used for propagation.

Abstract: Adeno-associated virus rh.20 sequences, vectors containing same, and methods of use are provided.

Abstract: Provided herein are improved vaccines for HSV-2.

Abstract: The invention described herein provides high throughput methods and reagents for generating transgenic animals (e.g., mammals) through introducing materials into gametes or preimplantation stage (e.g., one-cell embryo or zygotes) via electroporation, leading to genetically inheritable modification to the genome of the animal.

Abstract: A conditional rescue system generally includes a gene transfer system, a polynucleotide including a nuclease-resistant target coding region, and a coding region encoding a conditionally-lethal polypeptide. The gene transfer system is effective to integrate into host cell DNA. The polynucleotide including the nuclease-resistant target coding region is under transcriptional control of an inducible promoter. The coding region encoding a conditionally-lethal polypeptide is transcriptionally linked to the target coding region.

Abstract: This disclosure includes methods utilizing lignin or modified lignin compositions to reduce undesirable effects of unwanted microbial growth in fermentation media or animal feed, including lignin or modified lignin compositions that may be used in or for such methods.

Abstract: The present invention relates to processes for recovering butanol produced in a fermentative process using, for example, an ethanol production plant which has been reversibly-retrofitted for butanol production, that is, the ethanol production plant may be converted for butanol production, but can also revert to an ethanol production. The present invention also relates to processes for recovering butanol produced in a fermentative process in a butanol production plant that may be converted to ethanol production plant.

Abstract: Processes for producing ethanol comprise saccharifying cellulosic material with a cellulolytic enzyme composition and fermenting the saccharified cellulosic material with a fermenting microorganism to produce ethanol. The fermenting organism is Saccharomyces cerevisiae CIBTS1260 (deposited under Accession No. NRRL Y-50973 at the Agricultural Research Service Culture Collection (NRRL), Illinois 61604 U.S.A.) or a fermenting organism that has properties that the same or about the same as that of Saccharomyces cerevisiae CIBTS1260).

Abstract: In an aspect, the invention relates to compositions and methods production of n-butanol by aerobic hydrogen bacteria. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.

Abstract: Embodiments of the present invention relate to methods for the biosynthesis of di- or trifunctional C7 alkanes in the presence of isolated enzymes or in the presence of a recombinant host cell expressing those enzymes. The di- or trifunctional C7 alkanes are useful as intermediates in the production of nylon-7, nylon-7,x, nylon-x,7, and polyesters.

Abstract: A method for producing a dicarboxylic acid is provided. A dicarboxylic acid is produced by culturing a bacterium having a dicarboxylic acid-producing ability, which has been modified so that the expression of one or more of the yeeA gene, ynfM gene, yjjP gene, and yjjB gene is increased, in a medium, and collecting the dicarboxylic acid from the medium.

Abstract: A genetically engineered thermophilic bacterial cell that is facultative anaerobic and (S)-lactic producing including inactivation or deletion of the endogenous methylglyoxal synthase gene mgsA.

Abstract: The present invention provides methods for producing cannabinoid prodrugs. Also described are pharmaceuticals acceptable compositions of the prodrugs and a system for the large-scale production of the prodrugs.

Abstract: A method of preparing D-psicose includes a step of reacting D-fructose as a substrate and an epimerase thereof in microorganisms at a temperature of 40° C. or higher. The method may further includes inducing the microorganisms to have resting cells by culturing the microorganisms in a medium not containing the D-fructose before the reaction. The method of preparing D-psicose may significantly improve the production amount of D-psicose and production rate of D-psicose.

Abstract: An isothermal process for amplifying a nucleic acid target molecule that relies on an upstream primer, a downstream primer, a strand invasion system and an oligonucleotide, wherein the upstream and downstream primers are not substrates for the strand invasion system during the amplification process and do not amplify the target molecule independently of the strand invasion system, wherein the oligonucleotide is a substrate for the strand invasion system.

Abstract: Some aspects of this disclosure relate to the identification of reactive nucleic acids, such as, for example, reactive cellular RNAs. Minimal reactive motif consensus sequences and isolated reactive nucleic acids conforming to such consensus sequences are provided herein. Reactive probes that selectively form covalent bonds with reactive nucleic acids are also provided herein, as are methods of using such probes, for example, for labeling, identification, characterization, and/or quantification of reactive nucleic acids. This disclosure further provides fusion molecules comprising a reactive nucleic acid conjugated to a heterologous molecule of interest, such as a heterologous nucleic acid or protein. Methods and kits for generating and using such fusion proteins to track, detect, and/or quantify molecules of interest in a biological sample are also provided as are expression constructs encoding fusion molecules of reactive nucleic acids and heterologous nucleic acids of interest.

Abstract: A method for the production of nucleic acid encoding a target protein. The method comprises (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain n o more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex.

Abstract: Recombinant polypeptides having UDP-glycosyltransferase activities, including a 1,2-19-O-glucos glycosylation activity and a 1,2-13-O-glucos glycosylation activity for synthesizing of steviol glucosides, are provided. A method of producing a steviol glycoside composition using such recombinant polypeptide is also provided. Also disclosed are steviol glycosides referred to as rebaudioside Z1 and rebaudioside Z2.

Abstract: The present disclosure is directed to the properties of certain glycosyltransferase variants having N-terminal truncation deletions or internal deletions. Any of the mutants disclosed in here exhibit ?-2,6-sialyltransferase enzymatic activity in the presence of CMP-activated sialic acid as co-substrate, and in the presence of a suitable acceptor site. A fundamental finding documented in the present disclosure is that suchs enzyme are not only capable of catalyzing transfer of a sialidyl moiety but they are also capable of catalyzing hydrolytic cleavage of terminally bound sialic acid from a glycan.

Abstract: The present invention relates to the hemi sulfuric acid salt of D-phenylglycine methyl ester, to a method for the preparation of said salt and to the use of said salt in the enzymatic synthesis of antibiotics and of D-phenylglycine methyl ester free base.

Abstract: Various embodiments of the present invention relate to, among other things, systems for generating engineered water nanostructures (EWNS) comprising reactive oxygen species (ROS) and methods for inactivating at least one of viruses, bacteria, bacterial spores, and fungi in or on a wound of a subject in need thereof or on produce by applying EWNS to the wound or to the produce.

Abstract: The present invention relates to a method of interpreting different antibiogram images in which it is possible to recognize a phenotype of bacterial resistance relative to antibiotics by comparing photographs using a photographic image bank of the reference antibiogram without any need to interpret them using the EUCAST or CA-SFM interpretation data; providing that for a given phenotype, there is available a collection of photographs of a plurality of bacteria of the same species and of the same phenotype.

Abstract: The present disclosure describes methods for concentrating microorganisms with concentration agents in a sampling device and the sampling device described herein. More specifically, methods for concentrating microorganisms from large volume samples with concentration agents in a sampling device can provide for rapid, low cost, simple (involving no complex equipment or procedures), and/or effective processes under a variety of conditions.

Abstract: A test kit detects the presence of a target analyte in a fluid sample. In particular, the test kit includes reagents capable of detecting the target analyte of interest in breast milk. More particularly, the test kit is capable of detecting the presence of alcohol, caffeine, nicotine, drugs of abuse, therapeutic drugs, triglycerides, lactose, capsaicin, and gluten, for example, in breast milk.

Abstract: A detection system for determining alpha-methylacyl-CoA (AMACR) levels in a bodily sample includes at least one reaction solution for generating H2O2 upon combination with AMACR in the bodily sample and a biosensor for determining a level of generated H2O2. The reaction solution includes a (2R)-2-methylacyl-CoA epimer that can be chirally inverted by AMACR to a (2S)-2-methylacyl-CoA epimer and an enzyme that carries out beta oxidation with the (2S)-2-methylacyl-CoA epimer to generate hydrogen peroxide (H2O2).