Patents Issued in November 9, 2017
  • Publication number: 20170321177
    Abstract: Various aspects of the present invention relate to the control and manipulation of fluidic species, for example, in microfluidic systems. In one set of embodiments, droplets may be sorted using surface acoustic waves. The droplets may contain cells or other species. In some cases, the surface acoustic waves may be created using a surface acoustic wave generator such as an interdigitated transducer, and/or a material such as a piezoelectric substrate. The piezoelectric substrate may be isolated from the microfluidic substrate except at or proximate the location where the droplets are sorted, e.g., into first or second microfluidic channels. At such locations, the microfluidic substrate may be coupled to the piezoelectric substrate (or other material) by one or more coupling regions. In some cases, relatively high sorting rates may be achieved, e.g., at rates of at least about 1,000 Hz, at least about 10,000 Hz, or at least about 100,000 Hz, and in some embodiments, with high cell viability after sorting.
    Type: Application
    Filed: May 24, 2017
    Publication date: November 9, 2017
    Inventors: David A. Weitz, Thomas Franke, Achim Wixforth, Lothar Schmid, Jeremy Agresti, Adam R. Abate
  • Publication number: 20170321178
    Abstract: The present disclosure relates to the design, fabrication, and applications of a three-dimensional (3D) bioreactor for cell expansion and cell secreted substance production. The bioreactor is composed of non-random interconnected voids providing a continuous three-dimensional surface area for cell adherence and growth.
    Type: Application
    Filed: May 3, 2017
    Publication date: November 9, 2017
    Inventors: Jian LING, Jeffrey N. HARRIS, Michael J. RUBAL
  • Publication number: 20170321179
    Abstract: Algae cultivation systems and methods account for weather variations that can affect algae cultivation. In one system, an open raceway algae cultivation system includes a channel having a high section and a low liquid collection section. The channel is sloped to allow substantially all of an algae cultivation fluid in the high section to flow downwardly into the low liquid collection section. A barrier is removably positioned in the high section and a drain is positioned in the high section such that, when substantially all of the algae cultivation fluid has collected in the low liquid collection section, any rainwater that falls in the high section flows into the drain, without the rainwater mixing with the algae cultivation fluid in the low liquid collection section.
    Type: Application
    Filed: May 9, 2017
    Publication date: November 9, 2017
    Inventors: David A. Hazlebeck, Rodney Corpuz
  • Publication number: 20170321180
    Abstract: Scalable biomaterial-based bioreactors are described. In one embodiment, the bioreactor may comprise perforated plates stacked such that the assembled bioreactor has the necessary manifolds and chambers to transport gas and liquids to a biomaterial contained within the bioreactor, and to remove the reaction products. In another embodiment, single use bioreactors are described. Methods of operating the bioreactors are also described.
    Type: Application
    Filed: April 10, 2017
    Publication date: November 9, 2017
    Inventors: Zia Abdullah, Michael L. Dickens, Micah Paul McCreery, Randy L. Jones, Elvin Ray Beach, III, Jon-David S. Sears, Erin Suzanne Schultz, Stephanie Ann Smith, Paul E. George, II
  • Publication number: 20170321181
    Abstract: Algae harvesting and cultivating systems and methods for producing high concentrations of algae product with minimal energy. In an embodiment, a dead-end filtration system and method includes at least one tank and a plurality hollow fiber membranes positioned in the at least one tank. An algae medium is pulled through the hollow fiber membranes such that a retentate and a permeate are produced.
    Type: Application
    Filed: September 22, 2016
    Publication date: November 9, 2017
    Inventors: David A. Hazlebeck, William Rickman
  • Publication number: 20170321182
    Abstract: Algae harvesting and cultivating systems and methods for producing high concentrations of algae product with minimal energy. In an embodiment, a dead-end filtration system and method includes at least one tank and a plurality hollow fiber membranes positioned in the at least one tank. An algae medium is pulled through the hollow fiber membranes such that a retentate and a permeate are produced.
    Type: Application
    Filed: September 22, 2016
    Publication date: November 9, 2017
    Inventors: David A. Hazlebeck, William Rickman, Rodney Corpuz
  • Publication number: 20170321183
    Abstract: A cell isolation method includes: a cell trapping step of allowing a test liquid to pass through a cell trapping filter which has a plurality of through-holes in the thickness direction, thereby trapping isolation target cells contained in the test liquid on one surface of the cell trapping filter; a gel embedding step of introducing a stimulus-responsive hydrogel onto the one surface of the cell trapping filter on which the cells have been trapped in the cell trapping step, thereby embedding the cells in the stimulus-responsive hydrogel; a gel hardening step of applying a stimulus to the stimulus-responsive hydrogel in which the cells are embedded, thereby hardening the stimulus-responsive hydrogel; and a detachment step of detaching the stimulus-responsive hydrogel that was hardened in the gel hardening step from the cell trapping filter.
    Type: Application
    Filed: October 29, 2015
    Publication date: November 9, 2017
    Applicants: National University Corporation Tokyo University of Agriculture and Technology, Hitachi Chemical Company, Ltd.
    Inventors: Tomoko Yoshino, Tsuyoshi Tanaka, Tadashi Matsunaga, Ryo Negishi, Hisashige Kanbara, Seita Nakamura
  • Publication number: 20170321184
    Abstract: Algae harvesting and cultivating systems and methods for producing high concentrations of algae product with minimal energy. In an embodiment, a dead-end filtration system and method includes at least one tank and a plurality hollow fiber membranes positioned in the at least one tank. An algae medium is pulled through the hollow fiber membranes such that a retentate and a permeate are produced.
    Type: Application
    Filed: September 22, 2016
    Publication date: November 9, 2017
    Inventors: David A. Hazlebeck, William Rickman
  • Publication number: 20170321185
    Abstract: The present invention relates to the enzymatic synthesis of oligosaccharides, particularly, sialylated oligosaccharides comprising the carbohydrate moieties of the gangliosides GM3, GD3, and G.
    Type: Application
    Filed: May 31, 2017
    Publication date: November 9, 2017
    Applicant: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)
    Inventor: Eric SAMAIN
  • Publication number: 20170321186
    Abstract: The invention relates to a process for improving the solubility of dry cell culture media. Some dry powder cell culture media show poor dissolving properties and result in turbid solutions when they are dissolved in aqueous solutions. Using a stepwise procedure in which the amino acids present in the non-dissolving part are identified and added to a new batch in other particle sizes significantly reduces that problem.
    Type: Application
    Filed: November 13, 2015
    Publication date: November 9, 2017
    Applicant: Merck Patent GmbH
    Inventor: Joerg VON HAGEN
  • Publication number: 20170321187
    Abstract: The present invention concerns the separation, identification and characterization of active peptide fragments from peptones.
    Type: Application
    Filed: July 20, 2017
    Publication date: November 9, 2017
    Inventors: James Wilkins, Masaru Ken Shiratori, Tim Breece
  • Publication number: 20170321188
    Abstract: The present invention relates to methods of generating preparations of neural progenitor cells and retinal progenitor cells from populations of stem cells. These methods involve the administration of Tbx3 alone or in combination with Pax6. The preparations of neural and retinal progenitor cells prepared in accordance with the methods disclosed herein are suitable for use in methods of treating individuals having retinal disorders.
    Type: Application
    Filed: May 4, 2017
    Publication date: November 9, 2017
    Inventors: Andrea S. VICZIAN, Michael E. ZUBER
  • Publication number: 20170321189
    Abstract: Disclosed is a method of producing non-exhausted immature dendritic cells (DCs) originating front at two different, allogeneic donors. In the method, a mixture of allogeneic leukocytes, which allogeneic leukocytes have been obtained from at least two different, allogeneie donors is provided. Subsequently, allogeneic monocytes are isolated from the mixture of allogeneic leukocytes. Thereafter, non-exhausted immature DCs are generated from said isolated allogeneic monocytes.
    Type: Application
    Filed: June 15, 2017
    Publication date: November 9, 2017
    Inventors: Alex KARLSSON-PARRA, Bengt ANDERSSON
  • Publication number: 20170321190
    Abstract: The invention provides in certain embodiments, a method of generating a re-programmed differentiated epithelial cell comprising (a) contacting a non-stem somatic cell obtained from a subject with an effective amount of a de-differentiation agent to form a de-differentiated cell, and (b) transfecting the de-differentiated cell with an expression cassette comprising a promoter operably linked to a nucleic acid encoding a conversion agent to form a re-programmed differentiated cell. The invention also provides in certain embodiments, a method of generating a re-programmed differentiated epithelial cell comprising (a) contacting a non-stem somatic cell obtained from a subject with an effective amount of a de-differentiation agent to form a de-differentiated cell, and (b) contacting the de-differentiated cell with a conversion agent to form a re-programmed differentiated cell.
    Type: Application
    Filed: August 13, 2015
    Publication date: November 9, 2017
    Applicant: UNIVERSITY OF IOWA RESEARCH FOUNDATION
    Inventors: Brad A. Amendt, Thad Sharp
  • Publication number: 20170321191
    Abstract: The present invention provides a technique which enables organization of bone marrow cells by a simple method in a short period of time. A method for preparing a bone marrow cell aggregate, comprising adding a liquid containing a bone marrow cell population to a medium containing a swellable material and culturing the bone marrow cell population in the presence of the swellable material. A method for reassembling a bone marrow tissue, comprising adding a liquid containing a bone marrow cell population to a medium containing a swellable material and culturing the bone marrow cell population in the presence of the swellable material. According to common knowledge in the art, it has been considered difficult to reorganize once disintegrated bone marrow tissue without changing the cell composition (that is, without adding any adherent cell or extracellular matrix which will work as a “connecting material (binder)”). Indeed, it was impossible to aggregate bone marrow cells by conventional methods.
    Type: Application
    Filed: July 27, 2017
    Publication date: November 9, 2017
    Applicant: PUBLIC UNIVERSITY CORPORATION YOKOHAMA CITY UNIVERSITY
    Inventor: Nobuhiko KOJIMA
  • Publication number: 20170321192
    Abstract: Described herein are modified RNA virus gene segments and nucleic acids encoding modified RNA virus gene segments. Also described herein are recombinant RNA viruses comprising modified RNA virus gene segments and the use of such recombinant RNA viruses for the prevention and treatment of disease.
    Type: Application
    Filed: April 6, 2017
    Publication date: November 9, 2017
    Applicant: Icahn School of Medicine at Mount Sinai
    Inventor: Benjamin R. tenOever
  • Publication number: 20170321193
    Abstract: Genetically modified proteins with uricolytic activity are described. Proteins comprising truncated urate oxidases and methods for producing them, including PEGylated proteins comprising truncated urate oxidase are described.
    Type: Application
    Filed: July 13, 2017
    Publication date: November 9, 2017
    Inventors: Jacob Hartman, Simona Mendelovitz
  • Publication number: 20170321194
    Abstract: The present disclosure features methods and compositions for modulating lipid metabolism to achieve improved production and quality of recombinant products, such as next generation biologics. Modulation of lipid metabolism as described herein includes, for example, introducing a lipid metabolism modulator described herein to a cell or a cell-free system. Also encompassed by the present disclosure are engineered cells with improved production capacity and improved product quality, methods for engineering such cells, and preparations and mixtures comprising the products from such cells.
    Type: Application
    Filed: May 3, 2017
    Publication date: November 9, 2017
    Inventors: James Budge, Christopher Mark Smales, Tanya Jeane Knight, Robert Young
  • Publication number: 20170321195
    Abstract: Presented herein are methods and compositions for thermostable DNA polymerases that may be used to improve the PCR process and to improve the results obtained when using a thermostable DNA polymerase in other recombinant techniques such as DNA sequencing, nick-translation, and reverse transcription.
    Type: Application
    Filed: November 13, 2015
    Publication date: November 9, 2017
    Applicant: Illumina, Inc.
    Inventors: Misha Golynskiy, Molly He, Michael Previte, BeiBei Wang, Sergio Peisajovich
  • Publication number: 20170321196
    Abstract: A method, comprising the steps of providing a sample containing contaminating nucleoside diphosphates and/or nucleoside triphosphates, such as ATP and/or ATP analogues including deoxyribonucleoside triphosphates; reducing the amount of the contaminating nucleoside diphosphates and/or nucleoside triphosphates in the sample with an apyrase enzyme, wherein said apyrase enzyme is a Shigella flexneri apyrase; and performing an analysis of the sample, wherein said analysis comprises an assay that would have been affected by the contaminating nucleoside diphosphates and/or nucleoside triphosphates had they not been reduced in the reduction step.
    Type: Application
    Filed: November 6, 2015
    Publication date: November 9, 2017
    Inventors: Pavankumar Asalapuram, Aman Russom
  • Publication number: 20170321197
    Abstract: The present invention relates to a variant of a parent lipase, which parent lipase has the amino acid sequence of SEQ ID NO: 2, wherein the variant has lipase activity, comprises a substitution at a position corresponding to position 51 and comprises a substitution or remains unaltered at a position corresponding to position 96 of SEQ ID NO: 2. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Application
    Filed: December 8, 2015
    Publication date: November 9, 2017
    Applicant: Novozymes A/S
    Inventors: Christian Lundager Gylstorff, Christian Jackel, Jesper Vind, Allan Svendsen, Marco Malten, Thomas Agersten Poulsen
  • Publication number: 20170321198
    Abstract: The invention provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides non-naturally occurring or engineered RNA-targeting systems comprising a novel RNA-targeting CRISPR effector protein and at least one targeting nucleic acid component like a guide RNA.
    Type: Application
    Filed: April 7, 2017
    Publication date: November 9, 2017
    Inventors: Konstantin Severinov, Feng Zhang, Yuri I. Wolf, Sergey Shmakov, Ekaterina Semenova, Leonid Minakhin, Kira S. Makarova, Eugene Koonin, Silvana Konermann, Julia Joung, Jonathan S. Gootenberg, Omar O. Abudayyeh, Eric S. Lander
  • Publication number: 20170321199
    Abstract: The present invention relates to alpha-amylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Application
    Filed: November 20, 2015
    Publication date: November 9, 2017
    Applicant: NOVOZYMES A/S
    Inventors: Carsten Andersen, Iben Damager, Astrid Munch, Tine Hoff
  • Publication number: 20170321200
    Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: July 18, 2017
    Publication date: November 9, 2017
    Applicant: Novozymes Inc.
    Inventors: Yu Zhang, Ye Liu, Junxin Duan, Lan Tang
  • Publication number: 20170321201
    Abstract: The present invention concerns a method for selectively activating a thermostable hydrolase at a temperature above T1. The present invention provides compositions comprising a thermostable hydrolase and a temperature sensitive inhibitor, wherein said thermostable hydrolase and said temperature sensitive inhibitor form a hydrolase-inhibitor complex at a temperature below T1, but which dissociates at a temperature of about T1. The present invention also relates to uses of said compositions, and a method for preparing said compositions.
    Type: Application
    Filed: November 26, 2015
    Publication date: November 9, 2017
    Applicant: PURATOS NV
    Inventors: Geertrui Bosmans, Ellen Fierens, Kristof Brijs, Jan Delcour, Fabienne Verte, Jacques Georis, Valérie Dorgeo, Filip Arnaut
  • Publication number: 20170321202
    Abstract: The present invention provides engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.
    Type: Application
    Filed: May 5, 2017
    Publication date: November 9, 2017
    Inventors: Jovana Nazor, Vesna Mitchell, David Elgart, Katrina W. Lexa, Nikki Dellas, Robert Kevin Orr, Oscar Alvizo, Ravi David Garcia, Judy Victoria Antonio Viduya, Courtney Dianne Moffett
  • Publication number: 20170321203
    Abstract: Materials and methods for extracting and purifying proteins are provided. For example, the materials and methods provided herein can be used for extracting and purifying proteins that denature at low temperature.
    Type: Application
    Filed: July 27, 2017
    Publication date: November 9, 2017
    Inventors: Aniket Kale, Ranjani Varadan, Simon Christopher Davis
  • Publication number: 20170321204
    Abstract: Materials and methods for extracting and purifying proteins are provided. For example, the materials and methods provided herein can be used for extracting and purifying proteins that denature at low temperature.
    Type: Application
    Filed: July 27, 2017
    Publication date: November 9, 2017
    Inventors: Aniket Kale, Ranjani Varadan, Simon Christopher Davis
  • Publication number: 20170321205
    Abstract: The invention provides ubiquitin variants that specifically bind to SCF E3 ligases, and use of these variants to modulate the activity of SCF E3 ligases.
    Type: Application
    Filed: February 17, 2017
    Publication date: November 9, 2017
    Inventors: Maryna GORELIK, Sachdev SIDHU
  • Publication number: 20170321206
    Abstract: Provided are compositions comprising newly identified protein fragments of aminoacyl-tRNA synthetases, polynucleotides that encode them and complements thereof, related agents, and methods of use thereof in diagnostic, drug discovery, research, and therapeutic applications.
    Type: Application
    Filed: March 15, 2017
    Publication date: November 9, 2017
    Inventors: Leslie Ann Greene, Kyle P. Chiang, Fei Hong, Alain P. Vasserot, Wing-Sze Lo, Jeffry D. Watkins, Cheryl L. Quinn, John D. Mendlein
  • Publication number: 20170321207
    Abstract: Provided herein are a device and a method for preparation of immobilized proteins, enzymes or cells on a carrier to achieve the industrial batch production of the immobilized proteins, enzymes or cells.
    Type: Application
    Filed: October 28, 2015
    Publication date: November 9, 2017
    Inventors: Chung Hong Cheung, Jun Wang, Junming Chen, Guilin Wang
  • Publication number: 20170321208
    Abstract: Multi-stage acoustophoretic devices for continuously separating a second fluid or a particulate from a host fluid are disclosed. Methods of operating the multi-stage acoustophoretic devices are also disclosed. The systems may include multiple acoustophoretic devices fluidly connected to one another in series, each acoustophoretic device comprising a flow chamber, an ultrasonic transducer capable of creating a multi-dimensional acoustic standing wave, and a reflector. The systems can further include pumps and flowmeters.
    Type: Application
    Filed: May 3, 2017
    Publication date: November 9, 2017
    Inventors: Bart Lipkens, Walter M. Presz, JR., Brian Dutra, Rudolf Gilmanshin, Jason Dionne, Chris Leidel, Louis Masi, Goutam Ghoshal
  • Publication number: 20170321209
    Abstract: The present invention provides a method for the isolation of nucleic acid from microbial cells in an environmental sample. The method includes preparing a suspension of the environmental sample, lysing the suspended sample with a buffered solution, adding sodium dodecylsulfate solution to the lysed suspended sample, carrying out solvent extraction and separation to obtain an aqueous phase, reacting the aqueous phase with solvents to generate an insoluble precipitate containing nucleic acid, and isolating the nucleic acid therefrom, thereby releasing high molecular weight nucleic acid pellets from the cells.
    Type: Application
    Filed: July 19, 2017
    Publication date: November 9, 2017
    Applicant: UNIVERSITI PUTRA MALAYSIA
    Inventors: Mohd Ali Hassan, Meisam Tabatabaei, Mohd Rafein Zakaria, Abdul Raha Rahim, Ander-Denis G. Wright, Yoshihito Shirai, Norhani Abdullah, Mehdi Shamsara, Kenji Sakai
  • Publication number: 20170321210
    Abstract: The present invention provides a method of modifying a targeted site of a double stranded DNA, including a step of contacting a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a selected double stranded DNA and DNA glycosylase with sufficiently low reactivity with a DNA having an unrelaxed double helix structure (unrelaxed DNA) are bonded, with the double stranded DNA, to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site, without cleaving at least one strand of the double stranded DNA in the targeted site.
    Type: Application
    Filed: November 2, 2015
    Publication date: November 9, 2017
    Applicant: National University Corporation Kobe University
    Inventors: Keiji NISHIDA, Akihiko KONDO
  • Publication number: 20170321211
    Abstract: The present invention relates to innovative means of generating sequence-linked DNA fragments and subsequent uses of such linked DNA fragments for de novo haplotype-resolved whole genome mapping and massively parallel sequencing. In various embodiments described herein, the methods of the invention relate to methods of generating paired-end nucleic acid fragment sharing common linker nucleic acid sequences using a nicking endonuclease, a T7 endonuclease, a restriction enzyme, or a transposase, methods of analyzing the nucleotides sequences from the linked-paired-end sequenced fragments, and methods of de novo whole genome mapping. Thus, the methods of this invention allow establishing sequence contiguity across the whole genome, and achieving high-quality, low-cost de novo assembly of complex genomes.
    Type: Application
    Filed: July 18, 2017
    Publication date: November 9, 2017
    Applicant: DREXEL UNIVERSITY
    Inventors: Ming XIAO, Justin SIBERT
  • Publication number: 20170321212
    Abstract: Systems and methods for isolating DNA molecules that can include the steps of: providing a transposon mutant collection, the transposon mutant collection being stored in a plurality of wells; dispatching aliquots from each well in the transposon mutant collection to a set of pools in a combinatorial pooling array that uses pool address coordinates, the aliquots being dispatched to the set of pools based on a location of the aliquots within the transposon mutant collection; constructing an amplicon library, the amplicon library including a sequencing dataset; parsing the sequencing dataset to a set of putative transposon insertion locations and pool address coordinates; calculating a likelihood for each location and pool address coordinates within the set of putative transposon insertion locations and pool address coordinates using a Bayesian inference algorithm informed by internal self-consistency; and generating a progenitor collection catalog based on location and pool address coordinates verified in the c
    Type: Application
    Filed: May 5, 2017
    Publication date: November 9, 2017
    Inventors: Buz Barstow, Michael Baym, Lev Shaket, Isao Anzai, Oluwakemi Adesina
  • Publication number: 20170321213
    Abstract: The invention relates to a double-stranded ribonucleic acid (dsRNA) targeting a transthyretin (TTR) gene, and methods of using the dsRNA to inhibit expression of TTR.
    Type: Application
    Filed: December 15, 2016
    Publication date: November 9, 2017
    Inventors: Dinah Wen-Yee Sah, Gregory Hinkle, Rene Alvarez, Stuart Milstein, Qingmin Chen
  • Publication number: 20170321214
    Abstract: The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are structural information on the Cas protein of the CRISPR-Cas system, use of this information in generating modified components of the CRISPR complex, vectors and vector systems which encode one or more components or modified components of a CRISPR complex, as well as methods for the design and use of such vectors and components. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system. In particular the present invention comprehends optimized functional CRISPR-Cas enzyme systems.
    Type: Application
    Filed: June 12, 2017
    Publication date: November 9, 2017
    Inventors: Feng Zhang, Silvana Konermann, James Dahlman, Omar O. Abudayyeh
  • Publication number: 20170321215
    Abstract: The present invention provides short activating RNA molecules which up-regulate albumin production. The present invention also provides methods of up-regulating albumin production, such methods involving the use of short activating RNA molecules capable of increasing the expression of albumin. The present invention also provides the use of the short activating RNA molecules in therapy, such as treating or preventing a hyperproliferative disorder and/or a disorder characterised by hypoalbuminemia.
    Type: Application
    Filed: July 21, 2017
    Publication date: November 9, 2017
    Inventor: Pål Sætrom
  • Publication number: 20170321216
    Abstract: Provided herein are methods, compounds, and compositions for reducing expression of an AGPAT5 mRNA and protein in an animal. Also provided herein are methods, compounds, and compositions for reducing lipids, insulin resistance and/or glucose in an animal. Such methods, compounds, and compositions are useful to treat, prevent, delay, or ameliorate a cardiometabolic disease, disorder or condition, or a physiological marker thereof, in an individual in need.
    Type: Application
    Filed: November 13, 2015
    Publication date: November 9, 2017
    Applicants: The Regents of the University of California, Ionis Pharmaceuticals, Inc.
    Inventors: Aldons J. LUSIS, Brian PARKS, Richard LEE
  • Publication number: 20170321217
    Abstract: This present invention discloses an antisense oligonucleotide for splicing adjustment of mutant dopa decarboxylase gene which is complementary to SEQ ID NO: 1. This antisense oligonucleotide can modulate alternative splicing site of mutant dopa decarboxylase gene. It is helpful to research and develop drug to treat AADC deficiency symptom. This present invention also discloses a method to use said antisense oligonucleotide in vitro.
    Type: Application
    Filed: May 3, 2017
    Publication date: November 9, 2017
    Inventors: Chi-Ren TSAI, Ching-Shiang CHI, Hsiu-Fen LEE
  • Publication number: 20170321218
    Abstract: The present disclosure concerns aptamers of formula (I) capable of specifically binding to Factor XIa. The aptamers can be used to prevent, treat or alleviate the symptoms of thrombosis. The aptamers can also be used to detect Factor XIa in a sample and/or purify Factor XIa from a sample. The aptamers can further be used to identity putative therapeutic agents for the prevention, treatment or alleviation of symptoms associated with thrombosis.
    Type: Application
    Filed: May 4, 2017
    Publication date: November 9, 2017
    Inventors: William Peter Sheffield, David Apraku Donkor
  • Publication number: 20170321219
    Abstract: The oligonucleotide compositions of the present invention make use of combinations of oligonucleotides. In one aspect, the invention features an oligonucleotide composition including at least 2 different oligonucleotides targeted to a target gene. This invention also provides methods of inhibiting protein synthesis in a cell and methods of identifying oligonucleotide compositions that inhibit synthesis of a protein in a cell.
    Type: Application
    Filed: July 17, 2017
    Publication date: November 9, 2017
    Inventors: Tod WOOLF, Margaret Taylor
  • Publication number: 20170321220
    Abstract: The invention provides a fusion protein comprising an antigen binding domain linked to a bacteriophage decoration (Dec) protein along with a polynucleotide comprising the nucleic acid sequence of the fusion protein and a vector comprising the polynucleotide. Additionally, the invention provides a composition comprising the fusion protein and a virus-like particle (VLP), and a method of treating a disease in a mammal comprising administering a therapeutically effective amount of the composition to the mammal. The invention also provides a method of vaccinating against a disease comprising administering a composition comprising the fusion protein and a VLP encapsulating a protein.
    Type: Application
    Filed: May 4, 2017
    Publication date: November 9, 2017
    Applicant: INDIANA UNIVERSITY RESEARCH AND TECHNOLOGY CORPORATION
    Inventors: Trevor DOUGLAS, Benjamin Harold SCHWARZ, Ranjit KOLIYATT, Masaki UCHIDA, John Alexander AVERA
  • Publication number: 20170321221
    Abstract: The present invention resides in a method for the manufacture of a disulphide-requiring biopharmaceutical having an element of at least tertiary structure using wild type E. coli.
    Type: Application
    Filed: November 6, 2015
    Publication date: November 9, 2017
    Inventor: Colin ROBINSON
  • Publication number: 20170321222
    Abstract: Disclosed are yeast cells expressing TAR DNA-binding protein 43 (TDP-43) and methods of screening yeast cells to identify compounds that prevent or suppress TDP-43-induced toxicity, compounds that inhibit the formation or maintenance of cytoplasmic inclusions of TDP-43, genetic suppressors or enhancers of TDP-43-induced toxicity, and genetic suppressors or enhancers of the formation or maintenance of cytoplasmic inclusions of TDP-43. Compounds identified by such screens can be used to treat or prevent TDP-43 proteinopathies such as frontotemporal lobar degeneration or amyotrophic lateral sclerosis.
    Type: Application
    Filed: July 14, 2017
    Publication date: November 9, 2017
    Applicant: Whitehead Institute for Biomedical Research
    Inventors: Susan L. Lindquist, Aaron D. Gitler
  • Publication number: 20170321223
    Abstract: Plant metabolism and alkaloid levels can be regulated by transcription factors that regulate the nicotinic alkaloid biosynthetic pathway. In one embodiment, the disclosure provides a transcription factor that negatively regulates alkaloid biosynthesis, such as nicotine biosynthesis.
    Type: Application
    Filed: July 24, 2017
    Publication date: November 9, 2017
    Applicant: 22nd Century Limited, LLC
    Inventors: Jonathan PAGE, Andrea T. TODD
  • Publication number: 20170321224
    Abstract: The present invention relates to a Sendai virus or recombinant Sendai virus vector. In particular the present invention provides methods, vectors, formulations, compositions, and kits for a modified Enders strain Sendai viral vector. An immunogenic vector can be used in any in vitro or in vivo system. Moreover, some embodiments include vectors for imaging virus growth, location and transmission.
    Type: Application
    Filed: February 28, 2017
    Publication date: November 9, 2017
    Inventors: Julia Lea Hurwitz, Toru Takimoto, Charles John Russell, Allen Portner, Karen Slobod
  • Publication number: 20170321225
    Abstract: Some embodiments comprise methods, systems, and compositions to promote, improve and/or increase neuronal differentiation, oligodendrocyte differentiation, or neurological outcome or function in a patient in need thereof. Some embodiments also comprise the administration a composition comprising a pharmaceutically effective amount of one or more of a group comprising microRNA-146a, exosomes comprising microRNA-146a, a promoter of microRNA-146a expression, a microRNA-146a thymosin beta 4, and a phosphodiesterase 5 inhibitor to treat neurological conditions, diseases, or injuries in mammals, including in human beings.
    Type: Application
    Filed: May 19, 2017
    Publication date: November 9, 2017
    Inventors: Xianshuang Liu, Zhenggang Zhang, Michael Chopp, Lei Wang, Benjamin A.L. Buller
  • Publication number: 20170321226
    Abstract: Described herein are methods and vectors for rational, multiplexed manipulation of chromosomes within open reading frames (e.g., in protein libraries) or any segment of a chromosome in a cell or population of cells, in which various CRISPR systems are used.
    Type: Application
    Filed: June 22, 2017
    Publication date: November 9, 2017
    Inventors: Ryan T. GILL, Andrew GARST