Abstract: The present disclosure provides a bio-field effect transistor (BioFET) and a method of fabricating a BioFET device. The method includes forming a BioFET using one or more process steps compatible with or typical to a complementary metal-oxide-semiconductor (CMOS) process. The BioFET device may include a substrate; a gate structure disposed on a first surface of the substrate and an interface layer formed on the second surface of the substrate. The interface layer may allow for a receptor to be placed on the interface layer to detect the presence of a biomolecule or bio-entity.

Abstract: A liquid analyzer is one that performs analysis in a state of being immersed in a flowing analysis target liquid, and in order to simply make a good/bad determination and increase reliability, includes: a sensor adapted to, in a state where a responsive membrane is immersed in the flowing analysis target liquid, sense a predetermined component contained in the analysis target liquid; an analysis mechanism adapted to analyze the analysis target liquid with use of voltage generated in the sensor; and a resistance measurement mechanism adapted to, in the state where the responsive membrane is immersed in the flowing analysis target liquid, apply DC voltage to the responsive membrane to measure the resistance of the responsive membrane.

Abstract: The monitoring probe (1) comprises: a FR-4 substrate (2) with two faces; two copper tracks (3) arranged on one of the faces of the substrate (2), with an electrical contact terminal (7) on the outside of the probe (1); a conductive region (6) with reference electrode functions, with an electrical contact terminal (8) on the outside of the probe (1), occupying the entire other face of the substrate (2); a passivating material layer (5) partially covering the copper tracks (3) and leaving two free zones (12, 13) of said tracks (3) uncovered, one of said free zones (12) corresponding to the electrical contact terminal (7) of the two copper tracks (3); and two ISE sensor elements (4) that are sensitive to at least one of the parameters of interest to be monitored in the ground, and arranged in the other of the free zones (13) of the two copper tracks (3).

Abstract: Methods that allow independently applied pressures to a BGE reservoir and a sample reservoir for pressure-driven injection that can inject a discrete sample plug into a separation channel that does not require voltage applied to the sample reservoir and can allow for in-channel focusing methods to be used. The methods are particularly suitable for use with a mass spectrometer.

Abstract: Apparatus comprising an inlet region for receiving a gas mixture; an ionizer for supplying hydronium ions to the received gas mixture to generate ions, wherein the ionizer comprises a membrane for receiving the gas mixture, and wherein the membrane, preferably made of graphene oxide, is capable of generating hydronium ions from water; and an ion detector for detecting ions generated from the gas mixture.

Abstract: A technique facilitates examination of a tubing string which may comprise coiled tubing or other types of pipe. A sensor is positioned to monitor a pipe for a magnetic flux leakage signal indicating a defect in the pipe. The sensor outputs data on the magnetic flux leakage signal to a data processing system. Correlations between magnetic flux leakage signals and fatigue life of the pipe may be accessed by the data processing system and these correlations may be used to automatically predict a fatigue life of the pipe. Based on the determined fatigue life, an operation with respect to the pipe is selected and such operation may comprise continued normal use, repair, or removal from service.

Abstract: A system for inspecting thermal spray coated cylinder bores of aluminum alloy cylinder blocks, the system includes a failure detection apparatus, a heating apparatus, a cooling apparatus, and a control unit in electronic communication with each of the failure detection apparatus, the heating apparatus, and the cooling apparatus, and wherein the control unit includes a memory and a control logic sequence for operating the system.

Abstract: A method of detecting near surface inconsistencies in a structure is presented. A pulsed laser beam is directed towards the structure. Wide-band ultrasonic signals are formed in the structure when radiation of the pulsed laser beam is absorbed by the structure. The wide-band ultrasonic signals are detected to form data. The data is processed to identify a frequency associated with the near surface inconsistency.

Abstract: Various embodiments include an ultrasonic detection method, performed using an ultrasonic detection system having a set of corresponding transmitting phased array devices and receiving phased array devices, the method including: for each of a plurality of static positions about a portion of a machine rotor: transmitting, at a corresponding transmitting phased array device, a set of ultrasonic waves through the portion of the machine rotor, and receiving, at the corresponding receiving phased array device, the set of ultrasonic waves after transmission through the portion of the machine rotor, to obtain a set of ultrasonic detection information about the machine rotor; and forming an ultrasonic representation of the machine rotor by aligning the sets of the ultrasonic detection information about the machine rotor obtained from each of the plurality of static positions about the portion of the machine rotor.

Abstract: A processing apparatus includes a processing quality prediction unit capable of outputting workpiece surface processing signal, a storage unit capable of storing workpiece surface processing signal, and a workpiece surface information management unit capable of interpreting workpiece surface processing signal. The workpiece surface information management unit can convert the surface processing signal into data of workpiece surface quality. The workpiece surface information management unit can be utilized to respectively interpret the surface processing signals provided by the processing quality prediction unit and to convert them into data of the roughness degree of the workpiece surface texture, so as to clearly provide information regarding the quality of the workpiece surface and completely utilize the signals detected during the processing for increasing the value added of the processing apparatus and enhancing the efficiency of use of the processing apparatus.

Abstract: A sample injector configured to introduce a sample fluid into a mobile phase, wherein the mobile phase is to be driven by a mobile phase drive through a separation unit for separating compounds of the sample fluid in the mobile phase, wherein the sample injector comprises a metering device being operable for displacing fluid and for intaking a metered amount of the sample fluid into the sample injector, an injector valve being switchable for operating the sample injector selectively in a sample intake mode in which the metering device is operable to intake the sample fluid from a sample container, or a separation mode in which intaken sample fluid is driven between the mobile phase drive and the separation unit for separating the compounds, and a flow direction controller configured for defining an enabled flow direction of fluid displaced by the metering device and for defining a disabled flow direction.

Abstract: There is provided a system for performing a chromatographic separation of an analyte, methods of using the system to separate at least one component of an analyte and an eluent generator of use in the system. An exemplary system comprises: (a) an eluent generator comprising: (i) a housing configured to be pressurizable by gas, comprising an annular void defined by the housing, and a gas inlet for the gas and a gas outlet for the gas in fluid communication with the annular void; (ii) a membrane permeable to the gas defining an eluent flow channel disposed within the annular void, the eluent flow channel having an eluent precursor fluid inlet and an eluent outlet; (iii) a source of gas in fluidic communication with the gas inlet; (iv) a source of the eluent precursor fluid; and (b) a chromatography column disposed downstream of and in fluidic communication with the eluent outlet.

Abstract: The clamping device according to the invention for clamping at least two processing devices for a sample preparation is made up essentially of at least three clamping blocks arranged in the axial direction, at least two of which are designed as movable clamping blocks, and a displacement mechanism for displacing the movable clamping blocks between an open position for inserting the processing devices, and a clamping position in which the at least two processing devices are clamped one on top of the other in the axial direction between two clamping blocks in each case. The displacement mechanism is designed for displacing the at least two movable clamping blocks in opposite directions.

Abstract: Method for determining the influence of least a first and a second experimental parameter on a liquid chromatography protocol for purifying one or more target molecules from a sample, comprising the steps: —performing chromatography purifications of the sample at a plurality of different experimental conditions where at least the first and the second experimental parameter each are varied over a predetermined range, each purification being registered as a chromatogram by monitoring an output parameter indicative of the purification result during the purification; and —displaying in a graphical user interface at least a subset of the registered chromatograms as chromatogram-miniatures in an evaluation diagram wherein the position of each displayed chromatogram-miniature is determined by the experimental parameters for the corresponding purification, thereby allowing a user to visually determine trends and the influence of the experimental parameters on the liquid chromatography protocol.

Abstract: Methods, systems, devices, and products for evaluating a fluid. Methods include introducing a sample comprising the fluid to a solvating fluid at a point in a chamber associated with the instrument at a first time to create a heterogeneous admixture; measuring concentrations of each of a plurality of components in the admixture at a plurality of distances from the point in the chamber at, at least one additional time later than the first time, each of the plurality of distances being non-zero; and estimating a relative concentration for each of the plurality of components in the fluid by extrapolating the relative concentration of each of the plurality of components in the sample at the point at the first time using the measured concentrations in the admixture at the plurality of distances.

Abstract: A method for detecting a degree of hydrogenation of a liquid comprises one or more liquid hydrogen carriers, which can be hydrogenated, comprising: detecting a material property of the liquid and determining the degree of hydrogenation of the liquid on the basis of the detected material property of the liquid.

Abstract: A system for measuring cellular forces exerted on a surface comprising: a deformable sensor that has a deformable surface adapted to deform on application of a cellular force, and an optical detector for optically detecting deformation of the surface.

Abstract: The present subject matter discloses various exemplary projective capacitive micro structured electrode arrays for measuring the impedance and action potential of living cells in particular induced pluripotent stem cells that are subsequently differentiated into cardiomyocytes and neurons. In one exemplary system a projective capacitive electrode array with cardiomyocytes attached onto the electrodes is formed and electrical measurements of live cell responses when exposed to external stimulants, such as small molecule drugs or biologically active compounds is recorded.

Abstract: A method of forming a nanopore in a lipid bilayer is disclosed. A nanopore forming solution is deposited over a lipid bilayer. The nanopore forming solution has a concentration level and a corresponding activity level of pore molecules such that nanopores are substantially not formed un-stimulated in the lipid bilayer. Formation of a nanopore in the lipid bilayer is initiated by applying an agitation stimulus level to the lipid bilayer. In some embodiments, the concentration level and the corresponding activity level of pore molecules are at levels such that less than 30 percent of a plurality of available lipid bilayers have nanopores formed un-stimulated therein.

Abstract: A multi-phase system includes a phase-separated solution comprising at least two phases, each phase having a phase component selected from the group consisting of a polymer, a surfactant and combinations thereof, wherein at least one phase comprises a polymer, wherein the phases, taken together, represent a density gradient. Novel two-phase, three-phase, four-phase, five-phase, or six-phase systems are disclosed. Using the disclosed multi-phase polymer systems, particles, or other analyte of interest can be separated based on their different densities or affinities.

Abstract: A health monitoring toilet system collects urine in a toilet system which may resemble a traditional water toilet. The toilet system may include a urine capture basin for collecting urine as a user urinates as with a traditional toilet. The urine capture basin may include a urine entry aperture which is an orifice leading into a urine sample cell. A fiber optic spectrometer may analyze the urine in the urine sample cell to collect relevant health data. The system may include a controller which may store and analyze spectral data and compile longitudinal data over time. The system may be in connection with other such systems to compare the analysis of a user's urine with those of other users stored in other such health monitoring toilet systems.

Abstract: A method for measuring concentrations of blood cell components is provided. The method comprises: obtaining a blood sample from a subject, the blood sample comprising red blood cells (RBCs), white blood cells (WBCs), and platelets (PLTs); mixing the blood sample with a non-lysing aqueous solution to form a sample mixture comprising a predetermined tonicity; passing the sample mixture through a flow cell; emitting light towards the flow cell; measuring an amount of light absorbed by the RBCs; measuring an amount of light scattered by WBCs, and PLTs; determining a concentration of each of the RBCs, WBCs, and PLTs present in the sample mixture from the measured amount of light absorbed by the RBCs and scattered by the WBCs and PLTs.

Abstract: The present invention relates to cell-based methods for determining the biological activity of defibrotide. In particular, the invention provides a method for assessing the potency of defibrotide by assessing the viability of mammalian cells in the presence of at least one cytotoxic agent and one or more concentrations of defibrotide. Such methods are particularly useful for standardizing pharmaceutical compositions comprising defibrotide.

Abstract: Methods of identifying potential skin moisturizing actives for the treatment of dry skin and method of formulating a moisturizing skin care composition using actives identified by the method. Moisturizing agents can be identified by comparing the transcriptional profile of a skin tissue sample contacted by a test agent to a negative or positive control to determine if the regulation of certain genes corresponds to the appropriate direction of regulation indicated by the control. Agents identified as skin moisturizing agents can then be incorporated into a skin moisturizing composition.

Abstract: Disclosed herein are methods for diagnosing or predicting acute cellular and/or humoral rejection in a subject. In one example, a method of assessing organ rejection includes contacting a first sample comprising antigen presenting cells (APCs) obtained from a subject in need of or having received an organ transplant with a donor antigen from a donor under conditions sufficient to induce uptake of the donor antigen; contacting a second sample comprising APCs obtained from the subject in need of or having received an organ transplant with a third-party antigen under conditions sufficient to induce uptake of the third-party antigen; and determining an antigen presenting index by determining a ratio of uptake of the donor antigen in the first sample to uptake of the third-party antigen in the second sample, wherein the ratio of greater than one indicates organ rejection and the APCs are monocytes or monocyte-derived cells.

Abstract: Provided is a fluidic device, including: a porous flow path member; an absorbent member contacting the flow path member and configured to absorb a liquid; and a barrier member covering at least a portion of the absorbent member, wherein the absorbent member contains a liquid-absorbent polymer that absorbs the liquid, and a lyophilic polymer having lyophilicity to the liquid.

Abstract: A point of care immunization system based upon microfluidics and microtitration technologies to rapidly test a patient in order to ascertain an immunization profile so that vaccinations can be administered to address identified gaps. A point of care system comprised of uniquely shaped and color distinguishing sample and test cartridges, with said test cartridges configured to meet healthcare requirements of national governing bodies. A point of care system including an easy access vaccine storage device with indicators to provide data on viability of stored vaccines.

Abstract: The present invention provides methods, immunoassays, kits and devices pertaining to the detection of multiple biomolecules from single cells or other biological entities. It also enables the highly parallel detection of interacting biomolecules from such entities.

Abstract: A biological test strip with isolation structure includes a substrate having a testing portion and a measuring portion; an electrode disposed on the substrate and including an operation electrode and an auxiliary electrode, the operation electrode having a first operating end and a first reading end, the auxiliary electrode having a second operating end and a second reading end; a first flow confining member surrounding the first operating end; and a second flow confining member surrounding the first flow confining member and the second operating end, with a height of the second flow confining member larger than a height of the first flow confining member. Therefore, the flowing scope of the dropped liquid is confined, so as to fix the square measure of the dropped liquid contacting the electrode and thereby improve the analysis repeatability.

Abstract: Methods, systems, devices and apparatus for use in screening and/or selecting a library of nucleic acid molecules and/or nucleic acid tagged or encoded molecules for binding to or interaction with a target molecule or substance (e.g., for use in new compound or drug discovery) are described. In some embodiments the device comprises: (a) a spatially addressable array, said array comprising a plurality of separate and discrete locations thereon; (b) a plurality of different oligomers operably connected to said spatially addressable array at different ones of said separate and discrete locations; (c) a tag sequence which is complementary to, and is hybridized to, each of said oligomers; and (d) a candidate chemical operably connected to each of said tag sequences, wherein each of said discrete locations is a unique identifier for its corresponding oligomer; and wherein said tag sequence is a unique identifier for its connected candidate chemical.

Abstract: Systems and methods are provided for detecting one or more particles such as individual unlabeled molecules or single nanoparticles. In examples described herein, optical energy is introduced into a microtoroid or other microcavity to generate an evanescent field. The microcavity has a functionalized outer surface that has been functionalized with a chemically or biologically active substance such as an antibody, antigen or protein. An indication of a particle bound to the functionalized outer surface of the microcavity is then detected based on a reactive interaction between the particle and the evanescent field while using frequency locking, balanced detection and various filtering techniques. The frequency locking, balanced detection and filtering techniques reduce the signal-to-noise ratio (SNR) of the detection system so that single nanoparticles (e.g. 2.5 nanometers (nm) in radius) and individual molecules (e.g. 15.5 kilo-Dalton (kDa) in size) can be detected in aqueous solution in some examples.

Abstract: Devices and methods for the detection of antigens are disclosed. Devices and methods for detecting food-home pathogens are disclosed.

Abstract: Biosensors are made by attaching covalently or non-covalently at least one reporter group to one or more specific positions of a bacterial periplasmic binding protein (bPBP). Upon binding of ligand to the biosensor, there is a change in the signal transduced by the reporter group.

Abstract: Disclosed is an in vitro oral biofilm model of interdental spaces including: a support, and a plurality of substratum pairs attached to the support, wherein an oral biofilm is capable of forming on the substratum pairs, wherein the substratum pairs each include a first member and a second member, and wherein the first member and the second member of each substratum pair are arranged to form a space interval between the first member and second member. Methods for assessing the formation of oral biofilms using the in vitro oral biofilm model and methods for testing agents, such as oral compositions, on biofilm reduction using the present model are also provided.

Abstract: A phage specific antibody presenting particle, devices and methods related to detection of phage amplification are provided. Specifically, described herein are compositions and methods for detecting Listeria bacteria using an antibody that recognized A511 anti-Listeria phage, wherein the antibody is conjugated to a nanoparticle that can be detected by surface-enhanced Raman scattering (SERS).

Abstract: Lateral-flow assay and time-resolved fluorescence technologies provide a sensitive tool for detecting norovirus particles in a sample. A flow-through assay device employs norovirus and norovirus G2 antibodies. The lateral-flow assay includes a conjugate pad having conjugated probes. The conjugated probes are particles modified with a binding member that is configured to bind with a norovirus. The particles also have a fluorescent label. A general method of detecting the virus includes (i) preparing a sample with contaminants, (ii) processing the sample and depositing it onto the assay device, and (iii) measuring the fluorescence signal.

Abstract: Provided is a peptide for targeting gastric cancer, a composition for diagnosing radioresponsiveness-dependent gastric cancer using the peptide, and a drug delivery use of the peptide, wherein a functional peptide capable of targeting cancer has been discovered so as to implement personalized diagnosis and treatment for individual patients having cancer, consideration of problems occurring during treatment in which treatment cases of respective patients differ due to different therapeutic responses resulting from genetic differences in the individual patients.

Abstract: The present invention includes a semi-quantitative method for the detection of ENOX2 transcript variants from one or more anti-ENOX2 antibody-binding spots on a blot comprising the steps of: electrophoretically separating proteins from a concentrated blood, serum, or plasma sample from the subject; transferring the electrophoretically separated proteins to a substrate; separating the one or more ENOX2 transcript variants from the one or more anti-ENOX2 antibody-binding spots; and measuring an ENOX2-catalyzed conversion of an ionic silver or gold to a colloidal silver or gold detected by light scattering from the one or more anti-ENOX2 antibody binding spots on the substrate, wherein each of the one or more spots is indicative of the ENOX2 transcript variant. Also provided is the basis for a point of care test to detect ENOX2 transcript variants based on use of colloidal gold or silver complexes with ENOX2 as a rapid simple test for cancer presence.

Abstract: The present invention relates to the fields of medicine and molecular diagnostics. In particular, it relates to a novel method for the treatment, prevention and/or delay of cancer and to a diagnostic method involving detection of a novel auto-active and intracellular mutant of MET.

Abstract: The present disclosure provides methods of using certain biomarker expression profiles in the detection, diagnosis, prognosis, or development of treatment regimens for various cellular hyperproliferative disorders of the pancreas. For example, methods comprise detecting whether the concentration of ERBB2, ESR1, and TNC in a test biological sample from a subject is elevated as compared to a control.

Abstract: There are no reliable clinical bio-markers of survival prognosis, patient's risk stratification and treatment prediction for epithelial ovarian cancers(EOC). The most common type of the human EOC is a high grade serous EOC. This cancer is characterized with one of the lowest survival rates compared to other cancers. The present invention relates to an method for a prognosis of survival of a subject diagnosed with EOC, the method comprising determining in a sample of the subject gene expression level of at least one gene in the list of Evi1 pathway genes; and/or copy number of at least one gene in the MECOM locus; wherein the level against at least one expression threshold value will define the risk group of the subject and/or a risk of the disease progression after surgery treatment, and/or an effectiveness of post-surgery chemotherapy.

Abstract: The present invention aims to provide a method for detecting, with high sensitivity and specificity, ovarian clear cell adenocarcinoma, which is highly malignant, among benign and malignant ovarian tumors having various tissue types, and a reagent that can be used for the method. The present invention provides NT-TFPI2, which is a novel processed tissue factor pathway inhibitor 2 polypeptide, as a new detection marker for ovarian clear cell adenocarcinoma. The detection of ovarian clear cell adenocarcinoma is carried out by measuring the amount of NT-TFPI2, or the total amount of NT-TFPI2 and intact TFPI2. The reagent for detecting ovarian clear cell adenocarcinoma contains an antibody that specifically recognizes NT-TFPI2 and intact TFPI2.

Abstract: The present inventors have demonstrated that circulating auto-antibodies to cancer antigens hold promise as specific and sensitive biomarkers for the early detection of cancer. The present invention thus relates to methods of detecting cancer in a sample, comprising utilising a glycopeptide bait derived from human mucins with different cancer-associated O-glycans. Detected antibodies were demonstrated as glycopeptide specific, and it could be discriminated between e.g. colorectal cancer patients and healthy individuals. The inventors have also developed monoclonal antibodies based on the identified glycopeptides epitope baits, and demonstrated differential expression of the relevant target antigens. The invention thus, in a lock and key-based manner includes both glycopeptides and antibody tools for early detection of cancer, as well as methods of using the same for in situ visualisation and treatment of specific cancer types.

Abstract: A method of extracting organ metabolic functions using oral glucose tolerance tests (OGTTs) comprises: a step of measuring an amount of change over time in plasma glucose and plasma insulin at a regular time interval after a subject has consumed a certain quantity of a glucose solution; a step of extracting parameters associated with organ metabolism in a human body by applying the amount of change over time of the measured glucose and insulin to a human organ function model; and a step of diagnosing the metabolic function state of the subject using the extracted parameters.

Abstract: An object of the present invention is to provide a prediction marker in male animals for predicting behaviors of their offspring. Specifically, isolated H3K79me3 from a spermatocyte or a sperm of a male animal is used as a marker. When levels of methylation of H3K79me3 in a spermatocyte or a sperm which has been collected from male animals are analyzed, those with a lower level of trimethylation of H3K79me3 have a higher probability of their offspring having autism or exhibiting autism-like behaviors.

Abstract: The present invention relates to methods for diagnosing gestational diabetes mellitus (GDM) in a pregnant female.

Abstract: Contemplated test kits and methods for food sensitivity are based on rational-based selection of food preparations with established discriminatory p-value. Particularly preferred kits include those with a minimum number of food preparations that have an average discriminatory p-value of ?0.07 as determined by their raw p-value or an average discriminatory p-value of ?0.10 as determined by FDR multiplicity adjusted p-value. In further contemplated aspects, compositions and methods for food sensitivity are also stratified by gender to further enhance predictive value.

Abstract: Disclosed are a rigorous method and apparatuses for the comprehensive analysis of complex mixtures of organic molecules, specifically biopolymers, in some embodiments predominantly mixtures of proteins, which in some embodiments ultimately serve to obtain the information constituting a biomarker, or similar biological signature, or to monitor health or ageing. In some embodiments such signatures or patterns may indicate the presence, stage, or type of a disease, the expected or actual response to drugs. In some other embodiments such a method and apparatuses may serve to monitor and/or control cell development. In some other embodiments such a method and apparatuses may serve to develop and use means to measure, influence, or control the speed or degree of aging of cells or organisms. In some embodiments such a method and apparatuses may serve to determine at least in part the nature of biological hazards, bioterrorism threats, or biological weapons, including those based on bacteria and viruses.

Abstract: Subject of the present invention are biomarkers and methods for the identification of risk for subsequent cardiovascular event (e.g. coronary heart disease death, non-fatal myocardial infarction, ischemic stroke, hospitalizations for unstable angina pectotis, cardiac arrest) in patients that have experienced an acute coronary syndrome, comprising the detecting the level of NT-proBNP, homocysteine and CRP.

Abstract: The present invention provides compositions and methods for identifying subjects suffering from dry eye that can be treated by topical administration of a composition comprising lacritin or a bioactive fragment thereof. The application discloses in part that a ˜90 KDa deglycanated form of syndecan-1 is abundant in tears of normal individuals but not individuals suffering from dry eye, whereas a ˜25 kDa syndecan-1 fragment is detectable in dry, but not normal tears.