Abstract: Methods, compositions and kits for detecting microorganisms and/or profiling microbiota such as for example through use of nucleic acid amplification and detection.

Abstract: This invention provides methods for characterizing the amounts of nucleic acids, including plus/minus determinations, the use of different constructs, the use of a library and a reference library. Expression may also be compared in two or more samples using the methods of this invention. Also provided are heterophasic arrays comprising labeled positive copies of nucleic acids hybridized to the array and labeled negative copies of nucleic acids hybridized to the array, in which the labeled positive copies are separately quantifiable from the labeled negative copies.

Abstract: Methods of assaying cells of a cellular sample for the presence of a target nucleic acid are provided. Aspects of the methods include evaluating a cellular sample that has been contacted with a nuclease inhibitor for the presence of a target nucleic acid. Also provided are devices and kits that find use in practicing the methods described herein.

Abstract: The present invention relates to a proximity-probe based detection assay for detecting an analyte in a sample and in particular to a method that comprises the use of at least one set of at least first and second proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte directly or indirectly, wherein the nucleic acid domain of at least one of said proximity probes comprises a hairpin structure that can be unfolded by cleavage of the nucleic acid domain to generate at least one ligatable free end or region of complementarity to another nucleic acid molecule in said sample, wherein when the probes bind to said analyte unfolding said hairpin structure allows the nucleic acid domains of said at least first and second proximity probes to interact directly or indirectly.

Abstract: The present disclosure provides methods for detecting a single-stranded target RNA. The present disclosure provides methods of cleaving a precursor C2c2 guide RNA array into two or more C2c2 guide RNAs. The present disclosure provides a kit for detecting a target RNA in a sample.

Abstract: A method for sensing an analyte uses tethered particle motion. A functionalized particle [500] has a first state [504] in which the functionalized particle is bound to the surface and a second state [502] in which the functionalized particle is not bound to the surface, where the functionalized particle switches between the first and second states depending on the presence and absence of the analyte, thereby changing motion characteristics of the functionalized particle depending on the presence of the analyte. A spatial coordinate parameter of the functionalized particle is measured by a detector [516], and a processor [518] determines the presence/concentration of the analyte from changes in the measured spatial coordinate parameter.

Abstract: The present invention relates to detection of target nucleic acid sequences using different detection temperatures and reference values. The present invention employing different detection temperatures and reference values enables to detect a plurality of target nucleic acid sequences in conventional real-time manners even with a single type of label in a single reaction vessel.

Abstract: Methods for visualizing modified nucleotides in a specific nucleic acid sequence or specific nucleic acid sequence interactions in single cells, wherein the methods comprise coupling an in situ hybridization (ISH) reaction with a proximity ligation as say (PLA) reaction.

Abstract: This invention provides methods and systems for measuring the concentration of multiple nucleic acid sequences in a sample. The nucleic acid sequences in the sample are simultaneously amplified, for example, using polymerase chain reaction (PCR) in the presence of an array of nucleic acid probes. The amount of amplicon corresponding to the multiple nucleic acid sequences can be measured in real-time during or after each cycle using a real-time microarray. The measured amount of amplicon produced can be used to determine the original amount of the nucleic acid sequences in the sample. Also provided herein are biosensor arrays, systems and methods for affinity based assays that are able to simultaneously obtain high quality measurements of the binding characteristics of multiple analytes, and that are able to determine the amounts of those analytes in solution. The invention also provides a fully integrated bioarray for detecting real-time characteristics of affinity based assays.

Abstract: Disclosed is an in situ method for detecting spatial proximity relationships between nucleic acid sequences, such as DNA, in a cell. The method includes: providing a sample of one or more cells comprising nucleic acids; fragmenting the nucleic acids present in the cells that leaves 5? overhanging ends; filling in the overhanging ends with at least one labeled nucleotide; joining the filled in end of the fragmented nucleic acids that are in close physical proximity to create one or more end joined nucleic acid fragments having a junction; isolating the one or more end joined nucleic acid fragments using the labeled nucleotide; and determining the sequence at the junction of the one or more end joined nucleic acid fragments.

Abstract: The present invention provides, inter alia, methods and kits for identifying where a polypeptide of interest binds in a genome. The methods include a) carrying out a chromatin immunoprecipitation coupled to exonuclease digestion (ChIP-exo) process with an antibody against the polypeptide of interest; (b) extracting a polynucleotide fragment to which the polypeptide of interest binds; (c) carrying out a library preparation protocol adapted from an individual nucleotide resolution UV cross-linking and immunoprecipitation (iCLIP) process on the ChIP-exo processed polynucleotide fragment; and (d) sequencing the resulting polynucleotides. The kits include: (a) reagents sufficient to carry out ChIP-exo; (b) reagents sufficient to carry out the library preparation protocol adapted from the iCLIP process; and (c) instructions for use.

Abstract: Methods of sequencing molecules based on luminescence lifetimes and/or intensities are provided. In some aspects, methods of sequencing nucleic acids involve determining the luminescence lifetimes, and optionally luminescence intensities, of a series of luminescently labeled nucleotides incorporated during a nucleic acid sequencing reaction. In some aspects, the disclosure provides compositions comprising luminescently labeled nucleotides.

Abstract: This invention provides substrates for use in various applications, including single-molecule analytical reactions. Methods for propagating optical energy within a substrate are provided. Devices comprising waveguide substrates and dielectric omnidirectional reflectors are provided. Waveguide substrates with improved uniformity of optical energy intensity across one or more waveguides and enhanced waveguide illumination efficiency within an analytic detection region of the arrays are provided.

Abstract: Methods of monitoring and measuring dynamic adaptive immune cell responses are provided. High-throughput sequencing of T cell receptor and immunoglobulin loci is used to characterize the breadth of an effector cell response to a stimulus, such as a vaccine or infection. Unique responding effector cell clones and abundance thereof can be determined. Additionally, methods for determining the contribution of responding effector cells to the immunological memory compartment are provided.

Abstract: The present technology relates to biomarkers of oocyte quality and oocyte quality dealine, as well as methods in connection with such biomarkers, including method of determining the quality of an oocyte, kits for the same, and libraries, reproductive aging gene expression profiles and profile sets with information relating the same.

Abstract: Methods, compositions, and systems are provided for managing bovine subjects in order to maximize their individual potential performance and edible meat value, and to maximize profits obtained in marketing the bovine subjects. The methods and systems draw an inference of a trait of a bovine subject by determining the nucleotide occurrence of at least one bovine SNP that is identified herein as being associated with the trait. The inference is used in methods of the present invention to establish the economic value of a bovine subject, to improve profits related to selling beef from a bovine subject; to manage bovine subjects, to sort bovine subjects; to improve the genetics of a bovine population by selecting and breeding of bovine subjects, to clone a bovine subject with a specific trait, to track meat or another commercial product of a bovine subject; and to diagnose a health condition of a bovine subject.

Abstract: Described are methods for early noninvasive or minimally invasive detection of pathological changes in organ systems/organs/tissues/cells by quantifying organ system-/organ-/tissue-/cells type-enriched miRNA in bodily fluids.

Abstract: Provided are methods and kits for classifying a subject as being more likely to have benign multiple sclerosis (BMS) or as being more likely to have typical relapsing remitting multiple sclerosis (RRMS). Classification of multiple sclerosis disease course is performed by comparing a level of expression of at least one gene involved in the RNA polymerase I pathway in a cell of the subject to a reference expression data of said at least one gene obtained from a cell of at least one subject pre-diagnosed as having BMS and/or from a cell of at least one subject pre-diagnosed as having typical RRMS, thereby classifying the subject as being more likely to have BMS or as being more likely to have typical RRMS. Also provided are methods of diagnosing and treating multiple sclerosis and methods of monitoring treatment efficiency.

Abstract: The present invention provides methods and kits a) for preventing and/or treating cancer (e.g., colorectal cancer, neuroblastoma) that is linked, in part, to high levels of ODC activity and increased cellular polyamine content, b) for predicting cancer patient survival, especially cancer patient's whose cancer is linked, in part, to high levels of ODC activity and increased cellular polyamine contents, and c) for selecting the corresponding treatment options for such patients based on the allelic nucleotide sequence or SNP at positions +263 and/or +316 of the ODC1 gene as well as cancer treatment methods, in each case, which include the determination of the ODC1 genotype at the +263 and/or +316 positions, as a means to guide treatment selection.

Abstract: Analysis of 13,023 genes in 11 breast and 11 colorectal cancers revealed that individual tumors accumulate an average of ˜90 mutant genes but that only a subset of these contribute to the neoplastic process. Using stringent criteria to delineate this subset, we identified 189 genes (average of 11 per tumor) that were mutated at significant frequency. The vast majority of these genes were not known to be genetically altered in tumors and are predicted to affect a wide range of cellular functions, including transcription, adhesion, and invasion. These data define the genetic landscape of two human cancer types, provide new targets for diagnostic and therapeutic intervention and monitoring.

Abstract: Provided herein are cereblon isoforms and methods of their use as biomarkers for treating a disease, disorder, or condition with a treatment compound, e.g., thalidomide, lenalidomide, pomalidomide, 3-(5-amino-2-methyl-4-oxoquinazolin-3 (4H)-yl)-piperidine-2,6-dione, or (S)-3-(4-((4-(morpholinomethyl)benzyl)oxy)-1-oxoisoindolin-2-yl)piperidine-2,6-dione.

Abstract: The invention features compositions and methods for detecting and quantifying BRAFV600E in a sample of a subject. In particular embodiments, the invention provides a highly sensitive and precise quantitative method for diagnosis, post-operative surveillance, and monitoring evidence of disease over time in the adjuvant setting (i.e. treatment with BRAF-inhibitors).

Abstract: The invention relates to the identification and selection of differentially expressed transcripts (biomarker) in tumour cells. Specific determination of the level of these biomarkers can be used for screening and diagnosis of prostate cancer. Clinical application of assays based on these biomarker help reduce the high number of false positives of current standard screening assays.

Abstract: Disclosed is a use of methylation sites as a disease diagnosis marker, in particular relating to a use of methylation sites in a Y chromosome as a prostate cancer diagnosis marker. More particularly, the method and criterion for screening methylation sites in chromosomes associated with a disease are established in the present invention, and using prostate cancer as an example, six methylation sites in the Y chromosome associated with the prostate cancer diagnosis are screened out. Methylation sites in the Y chromosome screened can be used for early and rapid diagnosis of the disease as a prostate cancer diagnosis marker.

Abstract: This invention concerns pathological angiogenesis and cancer, related treatment methods, and related compositions. Also disclosed are related diagnosis kits and methods.

Abstract: The invention relates to pepper plants and lines having resistance to Powdery Mildew, caused by the fungus Leveillula taurica. The invention also relates to parts of pepper plants from lines having Powdery Mildew resistance, including seeds capable of growing pepper plants with Powdery Mildew resistance, and fruit. Methods for the identification, use and breeding of Powdery Mildew resistant pepper plants are also provided.

Abstract: Compositions and methods related to transgenic glyphosate tolerant Brassica plants are provided. Specifically, the present invention provides Brassica plants having a DP-073496-4 event which imparts tolerance to glyphosate. The Brassica plant harboring the DP-073496-4 event at the recited chromosomal location comprises genomic/transgene junctions within SEQ ID NO: 2 or with genomic/transgene junctions as set forth in SEQ ID NO: 12 and/or 13. The characterization of the genomic insertion site of the event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event are provided.

Abstract: The invention provides a transgenic Glycine max event MON87751, plants, plant cells, seeds, plant parts, progeny plants, and commodity products comprising event MON87751. The invention also provides polynucleotides specific for event MON87751, plants, plant cells, seeds, plant parts, and commodity products comprising polynucleotides for event MON87751. The invention also provides methods related to event MON87751.

Abstract: The present invention is directed to methods for reducing cross-reactivity between species employed in multiplexed immunoassays.

Abstract: Provided herein include compositions comprising a primer having the nucleic acid sequence of ACeIN-F3_c, a primer having the nucleic acid sequence of ACeIN-B3_a, a primer having the nucleic acid sequence of ACeIN-B3_b, a primer having the nucleic acid sequence of ACeIN-FIP_e, a primer having the nucleic acid sequence of ACeIN-FIP_f, a primer having the nucleic acid sequence of ACeIN-BIP (or ACeIN-BIP-song), a primer having the nucleic acid sequence of ACeIN-LF; and a primer having the nucleic acid sequence of ACeIN-LB. Also provided are methods of detecting human immunodeficiency virus (HIV) nucleic acids in a sample comprising performing reverse transcription-based loop mediated isothermal amplification (RT-LAMP) on a sample using the previously disclosed compositions.

Abstract: Modified G6PC (glucose-6-phosphatase, catalytic subunit) nucleic acids and glucose-6-phosphatase-? (G6Pase-?) enzymes with increased phosphohydrolase activity are described. Also described are vectors, such as adeno-associated virus (AAV) vectors, and recombinant AAV expressing modified G6Pase-?. The disclosed AAV vectors and rAAV can be used for gene therapy applications in the treatment of glycogen storage disease, particularly glycogen storage disease type Ia (GSD-Ia), and complications thereof.

Abstract: Reactor assemblies and methods for hydrolytic biomass conversion are disclosed herein. The reactor assemblies employ solely hydrostatic pressure, applied by the liquid biomass slurry, as means of providing pressure necessary to maintain high temperature conditions suitable for hydrolytic biomass conversion resulting in the formation of soluble pentose compounds. In embodiments, the methods employed in conjunction with the reactor assemblies yield soluble C5 carbohydrates in the forms of oligosaccharides and monosaccharides from one or more biomass slurries.

Abstract: A multiple chamber material-stirring lance and method used to treat molten metal in a ladle, the lance having a stirring gas chamber, and a plurality of gas permeable ports arranged at a terminal end of the gas chamber, and at least one material chamber positioned parallel to the gas chamber and terminating in a plurality of material ports. In use, the multiple chamber material-stirring lance is lowered into the ladle of molten metal, and gas and material are both introduced into a respective chamber and emitted through their respective ports. Stirring gas emitted through the gas permeable ports under a gas pressure between 40 and 600 cfm causes the stirring gas to create a boiling effect in the molten metal, drawing material into the stirring gas bubbles and away from the lance body, improving material dispersion efficiency and thus impurity extraction from the molten metal.

Abstract: A process for reducing flatness deviations in an alloy article is disclosed. An alloy article may be heated to a first temperature at least as great as a martensitic transformation start temperature of the alloy. A mechanical force may be applied to the alloy article at the first temperature. The mechanical force may tend to inhibit flatness deviations of a surface of the alloy article. The alloy article may be cooled to a second temperature no greater than a martensitic transformation finish temperature of the alloy. The mechanical force may be maintained on the alloy article during at least a portion of the cooling of the alloy article from the first temperature to the second temperature.

Abstract: A low-alloy steel has a composition including, by mass %, C: more than 0.45 and up to 0.65%; Si: 0.05 to 0.50%; Mn: 0.10 to 1.00%; P: up to 0.020%; S: up to 0.0020%; Cu: up to 0.1%; Cr: 0.40 to 1.50%; Ni: up to 0.1%; Mo: 0.50 to 2.50%; Ti: up to 0.01%; V: 0.05 to 0.25%; Nb: 0.005 to 0.20%; Al: 0.010 to 0.100%; B: up to 0.0005%; Ca: 0 to 0.003%; 0: up to 0.01%; N: up to 0.007%; and other elements. A microstructure consists of tempered martensite and retained austenite<2% in volume fraction, crystal grain size number being 9.0 or larger, number density of carbonitride-based inclusions with grain diameter of 50 ?m or larger being 10 inclusions/100 mm2 or smaller, and yield strength being 965 MPa or higher.

Abstract: Provided is a steel sheet for low-temperature service, which can be used at a wide temperature range from low temperature to room temperature in liquefied gas storage tanks and transport facilities. The steel sheet for low-temperature service has an excellent surface processing quality even after a processing processes is performed, such as a tension process. The a steel sheet contains manganese (Mn, 15-35 wt %), carbon (C, satisfying 23.6C+Mn?28 and 33.5C—Mn?23), copper (Cu, 5 wt % or less (excluding 0 wt %)), chrome (Cr, satisfying 28.5C+4.4Cr?57 (excluding 0 wt %)), titanium (Ti, 0.01-0.5 wt %), nitrogen (N, 0.003-0.2 wt %), the balance iron (Fe), and other inevitable impurities. Ti and N satisfy relational expression 1 below. [Relational expression 1] 1.0?Ti/N?4.5 (Mn, C, Cr, Ti, and N in the respective expressions mean wt % of respective ingredient contents).

Abstract: A method for manufacturing a non-oriented electrical steel sheet according to an exemplary embodiment of the present invention includes performing hot rolling on a slab after heating the slab to manufacture a hot rolled sheet; performing hot rolled sheet annealing on the hot rolled sheet; performing cold rolling on a steel sheet on which the hot rolled sheet annealing is completed to manufacture a cold rolled sheet; and performing cold rolled sheet annealing on the cold rolled sheet in which a difference between a cold rolled sheet annealing temperature in the cold rolled sheet annealing and a hot rolled sheet annealing temperature in the hot rolled sheet annealing is 100° C. or lower.

Abstract: A non-oriented electrical steel sheet according to an embodiment of the present invention includes Ti at 0.0030 wt % or less (excluding 0 wt %), Nb at 0.0035 wt % or less (excluding 0 wt %), V at 0.0040 wt % or less (excluding 0 wt %), B at 0.0003 wt % to 0.0020 wt %, and the remaining portion including Fe and other inevitably added impurities, wherein a value of ([Ti]+0.8[Nb]+0.5[V])/(10*[B]) may be 0.17 to 7.8.

Abstract: A high-strength high-toughness electric-resistance-welded steel pipe having high resistance to post-weld heat treatment is provided. The steel pipe having a composition including C: 0.01% to 0.12%, Si: 0.05% to 0.50%, Mn: 1.0% to 2.2%, P: 0.03% or less, S: 0.005% or less, Al: 0.001% to 0.10%, N: 0.006% or less, Nb: 0.010% to 0.100%, and Ti: 0.001% to 0.050%. The steel pipe having a structure composed of a 90% or more by volume of a bainitic ferrite phase as a main phase and 10% or less (including 0%) by volume of a second phase. The bainitic ferrite phase having an average grain size of 10 ?m or less, and the structure containing fine Nb precipitates having a particle size of less than 20 nm dispersed in a base material portion. The steel pipe having high strength, toughness, and high resistance that is maintained through post-weld heat treatment.

Abstract: An elongated steel element having a non-round cross-section and being in a work-hardened state, said elongated steel element having as steel composition: a carbon content ranging from 0.20 weight percent to 1.00 weight percent, a silicon content ranging from 0.05 weight percent to 2.0 weight percent, a manganese content ranging from 0.40 weight percent to 1.0 weight percent, a chromium content ranging from 0.0 weight percent to 1.0 weight percent, a sulfur and phosphor content being individually limited to 0.025 weight percent, contents of nickel, vanadium, aluminium, molybdenum or cobalt all being individually limited to 0.5 weight percent, the remainder being iron and unavoidable impurities, said steel having martensitic structure that comprises martensitic grains, wherein a fraction of at least 10 volume percent of martensitic grains is oriented.

Abstract: A method for recovering metal values from a molten slag composition includes atomizing the slag with an oxygen-containing gas in a gas atomization apparatus, to produce solid slag granules. Oxygen in the atomizing gas converts metals to magnetic metal compounds, thereby magnetizing the metal-containing slag granules. These metal-containing slag granules are then magnetically separated. Larger amounts of metals may be removed by passing the molten slag through a pre-settling pan with an adjustable base, and/or discontinuing atomization where the metal content of the slag exceeds a predetermined amount. Solid slag granules produced by atomization may be charged to a recovery unit for recovery of one or more metal by-products. An apparatus for recovering metal values from molten slag includes a gas atomization apparatus, a flow control device for controlling the flow of atomizing gas, a control system, and one or more sensors to detect metal values in the slag.

Abstract: A method of recovering metals from electronic waste comprises providing a powder comprising electronic waste in at least a first reactor and a second reactor and providing an electrolyte comprising at least ferric ions in an electrochemical cell in fluid communication with the first reactor and the second reactor. The method further includes contacting the powders within the first reactor and the second reactor with the electrolyte to dissolve at least one base metal from each reactor into the electrolyte and reduce at least some of the ferric ions to ferrous ions. The ferrous ions are oxidized at an anode of the electrochemical cell to regenerate the ferric ions. The powder within the second reactor comprises a higher weight percent of the at least one base metal than the powder in the first reactor. Additional methods of recovering metals from electronic waste are also described, as well as an apparatus of recovering metals from electronic waste.

Abstract: The method for separation of metals from electronic cards includes a step of processing the electronic cards in an aqueous medium under supercritical conditions. The method also a later step of crushing solid materials coming from the treatment under supercritical conditions.

Abstract: A method for producing an aluminum-copper alloy from a lithium ion secondary battery includes supplying an aluminum source and a copper source derived from an aluminum-based positive electrode collector and a copper-based negative electrode collector, respectively, into a furnace, and melting the aluminum source and the copper source in a way that produces an alloy substantially free of a lithium component.

Abstract: Microelectronic substrates having copper alloy conductive routes to reduce warpage due to differing coefficient of thermal expansion of the components used to form the microelectronic substrates. In one embodiment, the conductive routes of the microelectronic substrate may comprise an alloy of copper and an alloying metal of tungsten, molybdenum, or a combination thereof. In another embodiment, the conductive routes of the microelectronic substrate may comprise an alloy of copper, an alloying metal of tungsten, molybdenum, or a combination thereof, and a co-deposition metal of nickel, cobalt, iron, or a combination thereof. In still another embodiment, the copper alloy conductive routes may have copper concentrations which are graded therethrough, which may enable better pattern formation during a subtractive etching process used to form the copper alloy conductive routes.

Abstract: A titanium composite material includes a titanium matrix material and a powder reinforced composite material with a volume ratio of 10%-70%. The titanium matrix material is disposed at an ? phase, a ? phase, an ?+? phase, an omega phase, or an intermetallic ?-1, ?-2, ?-3 phase. The powder reinforced composite material is selected from a ceramic powder material, a powder material with electric features or a magnetic powder material. Thus, the powder reinforced composite material is added into and combined with the titanium matrix material to form the titanium composite material by casting, agglomerating or pressing, so that the titanium matrix material contains the physical, chemical or electric features of the titanium matrix material and the powder reinforced composite material.

Abstract: The present invention provides a ?-type titanium alloy that includes, by mass %, when Al: 2 to 5%, 1) Fe: 2 to 4%, Cr: 6.2 to 11%, and V: 4 to 10%, 2) Fe: 2 to 4%, Cr: 5 to 11%, and Mo: 4 to 10%, or 3) Fe: 2 to 4%, Cr: 5.5 to 11%, and Mo+V (total of Mo and V): 4 to 10% in range, and a balance of substantially Ti. These include Zr added in amounts of 1 to 4 mass %. Furthermore, by making the oxygen equivalent Q 0.15 to 0.30 or leaving the alloy in the work hardened state or by applying both, the tensile strength before aging heat treatment can be further increased.

Abstract: An alloy for structural direct-writing additive manufacturing comprising a base element selected from the group consisting of aluminum (Al), nickel (Ni) and a combination thereof, and a rare earth element selected from the group consisting of cerium (Ce), lanthanide (La) and a combination thereof, and a eutectic intermetallic present in said alloy in an amount ranging from about 0.5 wt. % to 7.5 wt. %. The invention is also directed to a method of structural direct-write additive manufacturing using the above-described alloy, as well as 3D objects produced by the method. The invention is also directed to methods of producing the above-described alloy.

Abstract: A high-strength spring steel coil spring of a vehicle suspension, having excellent corrosion resistance, may include 0.4 to 0.9 wt % of C, 0.9 to 2.3 wt % of Si, 0.5 to 1.2 wt % of Mn, 0.6 to 1.5 wt % of Cr, 0.01 to 0.5 wt % of Mo, 0.01 to 0.9 wt % of Ni, 0.5 wt % or less (excluding 0 wt %) of V, 0.5 wt % or less (excluding 0 wt %) of Nb, 0.3 wt % or less (excluding 0 wt %) of Ti, 1.0 wt % or less (excluding 0 wt %) of Co, 0.1 wt % or less (excluding 0 wt %) of B, 0.3 wt % or less (excluding 0 wt %) of W, 0.3 wt % or less (excluding 0 wt %) of Cu, 0.3 wt % or less (excluding 0 wt %) of Al, 0.03 wt % or less (excluding 0 wt %) of N, 0.003 wt % or less (excluding 0 wt %) of O, and a remainder of Fe and inevitable impurities.

Abstract: An ultrahigh-strength spring steel, used as steel for a vehicle engine valve spring may include 0.5 to 0.7 wt % of C, 1.3 to 2.5 wt % of Si, 0.6 to 1.2 wt % of Mn, 0.6 to 1.5 wt % of Cr, 0.01 to 0.5 wt % of Mo, 0.01 to 0.9 wt % of Ni, 0.5 wt % or less (excluding 0 wt %) of V, 0.5 wt % or less (excluding 0 wt %) of Nb, 0.3 wt % or less (excluding 0 wt %) of Ti, 1.0 wt % or less (excluding 0 wt %) of Co, 0.1 wt % or less (excluding 0 wt %) of B, 0.3 wt % or less (excluding 0 wt %) of W, 0.3 wt % or less (excluding 0 wt %) of Cu, 0.3 wt % or less (excluding 0 wt %) of Al, 0.03 wt % or less (excluding 0 wt %) of N, 0.003 wt % or less (excluding 0 wt %) of O, and a remainder of Iron (Fe) and inevitable impurities.