Abstract: Provided is a method for detecting a stem cell based on an undifferentiated sugar chain marker having a specific sugar chain structure, wherein the stem cell is detected by detecting podocalyxin contained in a culture supernatant or a lysate of cells by a “lectin-antibody sandwich method” using a combination of a lectin and an antibody and having high sensitivity, the method including steps of: contacting the culture supernatant or the lysate, a lectin capable of binding to a sugar chain represented by (Formula 1) or (Formula 2), and an antibody capable of binding to keratan sulfate to form a complex composed of the lectin, podocalyxin and the antibody; and detecting the complex. wherein R1 represents an OH group or any sugar chain and R2 represents an OH group or any sugar chain, protein, lipid, or another molecule. wherein R1 represents an OH group or any sugar chain and R2 represents an OH group or any sugar chain, protein, lipid, or another molecule.

Abstract: To provide a marker for determining sensitivity to an anti-cancer agent, which can determine whether or not a patient has a therapeutic response to the anti-cancer agent, and cancer therapeutic means employing the marker.

Abstract: A method of measuring a metabolic activity (MA) of a cell is provided. The method comprising independently measuring in an extracellular environment of the cell, time-dependent acidification profiles due to secretion of: (i) non-volatile soluble metabolic products; (ii) non-volatile soluble metabolic products and volatile soluble metabolic products; and (iii) volatile soluble metabolic products; wherein at least one of the time dependent acidification profiles is indicative of the metabolic activity of the cell. Also provided are clinical methods which make use of the assay.

Abstract: The present disclosure provides methods and systems for detecting a presence or absence of one or more analytes in small volumes of samples. The detected presence or absence of the one or more analytes can be used for a variety of applications including biomonitoring.

Abstract: Device for use in a biosensor comprising a multisite array of test sites, the device being useful for modulating the binding interactions between a (biomolecular) probe or detection agent and an analyte of interest from a biological by modulating the pH or ionic gradient near the electrodes in such biosensor. The device provides a biosensor which is more accurate, reliable and the results of which are more reproducible. Analytic methods for more accurately measuring an analyte of interest in a biological sample are also provided.

Abstract: The present invention provides kits and assay methods for the early detection of pathogens, precise identification of the etiologic agent, and improved disease surveillance. More specifically, the present invention discloses an immunoassay leading to the rapid and simultaneous detection of antibodies to a wide range of infectious pathogens in biological fluids of infected patients. This immunoassay involves the covalent and oriented coupling of fusion proteins comprising an AGT enzyme and a viral antigen on an identifiable solid support (e.g. fluorescent microspheres), said support being previously coated with an AGT substrate. This coupling is mediated by the irreversible reaction of the AGT enzyme on its substrate. The thus obtained antigen-coupled microspheres show enhanced capture of specific antibodies as compared to antigen-coupled microspheres produced by standard amine coupling procedures.

Abstract: The present invention provides an analyte detection system for detecting target analytes in a sample. In particular, the invention provides a detection system in a rotor or disc format that utilizes a centrifugal force to move the sample through the detection system. Methods of using the rotor detection system to detect analytes in samples, particularly biological samples, and kits comprising the rotor detection system are also disclosed.

Abstract: The present relates to an analyte detection device comprising: a simple chamber for storing a mixture solution of a sample comprising an analyte and a reactant comprising particles; a detection chamber for storing a detection solution; and a channel placed between the sample chamber and the detection chamber to prevent the mixture solution and the detection solution from being mixed with each other, the analyte detection device characterized by detecting the analyte by moving the particles from the sample chamber to the detection chamber using moving means.

Abstract: An assay device includes: a liquid sample zone; a reagent zone downstream and in fluid communication with the sample zone that includes a reagent cell having a line of symmetry in the direction of fluid flow; a reagent material in the reagent cell, wherein the reagent material includes a first reagent material located at the axis of symmetry and is left-right symmetric, and a second and third reagent material having a substantially identical shape and volume and located in mirror locations from the line of symmetry; a detection zone in fluid communication with the reagent zone; and a wicking zone in fluid communication with the detection zone having a capacity to receive liquid sample flowing from the detection zone. The sample addition zone, the detection zone and the wicking zone define a fluid flow path.

Abstract: The present disclosure relates to the field of molecular biology and more specifically to methods for detecting anti-carbamylated protein (anti-CarP) antibodies in the serum of rheumatoid arthritis (RA) patients.

Abstract: A method for treating multiple sclerosis comprises applying peripheral blood from a patient or subject to an apheresis column loaded with a solid support comprising one or more binding reagents capable of specifically binding to a chemokine receptor, optionally the chemokine receptor CCR2, CCR6, CCR3, CCR5, CCR1, CXCR3 and/or CCR9 immobilized directly or indirectly on the support thus removing one or more chemokine receptor, optionally CCR2, CCR6, CCR3, CCR5, CCR1, CXCR3 and/or CCR9 expressing cells from the peripheral blood of the patient or subject. Various companion diagnostic methods and useful binding reagents are also described.

Abstract: A method for treating a respiratory conditions comprises applying peripheral blood from a patient or subject to an apheresis column loaded with a solid support comprising one or more binding reagents capable of specifically binding to a chemokine receptor, optionally the chemokine receptor CCR2, CCR1, CCR3, CCR5, CXCR1, CXCR2 and/or CCR7 immobilized directly or indirectly on the support thus removing one or more chemokine receptor, optionally CCR2, CCR1, CCR3, CCR5, CXCR1, CXCR2 and/or CCR7 expressing cells from the peripheral blood of the patient or subject. Various companion diagnostic methods and useful binding reagents are also described.

Abstract: A method for treating sepsis and/or respiratory distress syndrome (RDS) comprises applying peripheral blood from a patient or subject to an apheresis column loaded with a solid support comprising one or more binding reagents capable of specifically binding to a chemokine receptor, optionally the chemokine receptor CCR5, CXCR1, CXCR2, and/or CCR2 immobilized directly or indirectly on the support thus removing one or more chemokine receptor, optionally CCR5, CXCR1, CXCR2, and/or CCR2 expressing cells from the peripheral blood of the patient or subject. Various companion therapeutic methods and useful binding reagents are also described.

Abstract: A method for treating cancer comprising applying peripheral blood from a patient or subject to an apheresis column loaded with a solid support comprising one or more binding reagents capable of specifically binding to a chemokine receptor, optionally the chemokine receptor CCR7, CCR5, CCR6, CCR8, CXCR4, CXCR7, CCR4, CCR9, CCR10, CXCR3 or CXCR5 or to a Treg receptor immobilized directly or indirectly on the support thus removing one or more chemokine receptor, optionally CCR7, CCR5, CCR6, CCR8, CXCR4, CXCR7, CCR4, CCR9, CCR10, CXCR3 or CXCR5 or Treg receptor expressing cells from the peripheral blood of the patient or subject. Various companion diagnostic methods and useful binding reagents are also described.

Abstract: A method for treating mental disorders such as schizophrenia, depression and bipolar disorder comprises applying peripheral blood from a patient or subject to an apheresis column loaded with a solid support comprising a binding reagent capable of specifically binding to a chemokine receptor, optionally the chemokine receptor CCR9, CCR1, CCR3 and/or CCR5 immobilized directly or indirectly on the support thus removing chemokine receptor, optionally CCR9, CCR1, CCR3 and/or CCR5 expressing cells from the peripheral blood of the patient or subject. Various companion diagnostic methods and useful binding reagents are also described.

Abstract: Guanidine-functionalized perlite particles are provided. Nonwoven articles are also provided, including a fibrous porous matrix and guanidine-functionalized perlite particles enmeshed in the fibrous porous matrix. Laminated articles are additionally provided, including a first substrate and a second substrate sealed to the first substrate along at least a portion of a perimeter of the first substrate. The laminated article further includes guanidine-functionalized perlite particles disposed between the first substrate and the second substrate. Methods of detecting microorganisms or target cellular analytes in a fluid sample using guanidine-functionalized particles or laminated articles are also provided.

Abstract: Methods are provided for the rapid and efficient separation of target bioentities.

Abstract: The present invention is related to a method of detecting an endotoxin in a sample using a chromogenic assay, the method comprising: (a) contacting the sample with a reagent comprising limulus amebocyte lysate (LAL) and a chromogenic substrate; and (b) measuring a chromogenic effect resulting from a change in the chromogenic substrate in the presence of endotoxin in the sample; wherein the LAL is substantially free of coagulogen. The method also relates to compositions and kits comprising LAL substantially free of coagulogen, and methods of making such.

Abstract: The present invention provides a method for detecting and treating hematologic malignancies including leukemia such as acute lymphoblastic leukemia (ALL) in a subject and a method for determining disease prognosis among leukemia patients by detecting CD9 expression. A kit and device useful for such methods are also provided. In addition, the present invention provides a composition for treating hematologic malignancies such as leukemia by suppressing CD9 expression or activity.

Abstract: The present invention relates to an apparatus and a method for determining breast cancer prognosis and whether to use chemotherapy, and more specifically, to an apparatus and method capable of predicting prognosis and chemotherapeutic effects, which are necessary in determining a treatment plan for a breast cancer patient, by use of progesterone receptor immunostaining results and Ki67 protein immunostaining results. The method for determining breast cancer prognosis according to the present invention can be performed at low costs compared to a conventional method for determining breast cancer prognosis, and can provide a standardized index in predicting breast cancer prognosis and chemotherapeutic effects, due to its relatively high accuracy, and ultimately can contribute to improving national health and welfare.

Abstract: A method for the diagnosis of the endometrial carcinoma is disclosed.

Abstract: Provided herein are compositions and methods for the treatment of cancer by inhibition of ?-catenin or a ?-catenin pathway. In particular, inhibitors of ?-catenin and/or the Wnt/p-catenin signaling pathway are employed prevent or reverse evasion of immune response or immuno-therapy by cancers.

Abstract: A method for predicting the degree of weight loss attainable by applying one or more dietary interventions to a subject, said method comprising determining the level of one or more biomarkers in one or more samples obtained from the subject, wherein the biomarkers are selected from fructosamine and factor VII.

Abstract: The present invention provides a panel of at least five clinical analyses or tests (using serum samples) to determine the risk of pediatric acute-onset neuropsychiatric syndrome (PANS) and/or pediatric autoimmune neuropsychiatric disorder associated with streptococcal infection (PANDAS) in an individual. These include enzyme linked immunosorbent assays (ELISAs) to measure antibody titers against neuronal antigens present in the brain; the neuronal antigens include lysoganglioside, tubulin, dopamine receptor D1, dopamine receptor D2, serotonin receptor 5HT2A, and serotonin receptor 5HT2C. Antibody titers against at least four of these neuronal antigens are required in the present methods; preferably antibody tiers against all of these neuronal antigens are measured. A final assay is used to quantify calcium/calmodulin-dependent protein kinase activity using a neuronal cell line.

Abstract: The invention relates to biomarkers associated with preterm delivery. More specifically, the invention provides methods of measuring biomarkers found in women that are at risk for preterm delivery.

Abstract: Polyethylene glycol (PEG) is often conjugated with therapeutic proteins to enhance their PK properties. PEG may, however, be immunogenic, and the presence of PEG in food and cosmetics is believed to result in pre-existing anti-PEG antibodies in humans. Polyclonal and monoclonal antibodies reactive to PEG are provided for use in immunogenicity assay development to detect such anti-drug antibodies. Such antibodies exhibit preferential binding based on the size of PEG with molecular weight ranging from 350 daltons to 40 kD. Anti-PEG antibodies of the invention are engineered to comprise human Fc regions to enable non-bridging immunoassay formats.

Abstract: Embodiments of the present invention are directed to the use of NHE3 as an early biomarker for radiation biodosimetry. In other embodiments, the present invention relates to the use of NHE3 as a biomarker for determining the absorbed radiation dose in a subject who has been exposed to a known or unknown dose of ionizing radiation. Further embodiments relate to the use of NHE3 as a biomarker for determining effectiveness of a therapy for reducing radiation toxicity. Advantageously, the diagnostic and prognostic assays of the present invention are rapid, sensitive, and non-invasive, rendering it useful in civilian and military industries.

Abstract: Provided are methods of determining if a subject has a cardiac disorder or is at risk for developing a cardiac disorder.

Abstract: Described herein are methods of identifying a mammal having a neurological disease or disorder, such as AD or MCI, or at risk for developing a neurological disease or disorder, such as AD or MCI. Provided herein are also methods of monitoring the progression of a neurological disease or disorder in a patient or monitoring the effectiveness of therapeutic agent or treatment of a patient having a neurological disease or disorder.

Abstract: The present invention relates to a method for applying a principle of Deterministic Lateral Displacement (DLD), and for separating cells depending on the particle sizes on the basis of the DLD principle after capturing targeted cells or untargeted cells on a carrier and thereby increasing particle sizes.

Abstract: The invention provides improved methods for analyzing cardiovascular disease risk. According to the invention, an algorithm that considers LDL and HDL subfractions, along with Lp(a) provides significant improvement in predicting CVD versus standard assays that include standard risk factors. Methods of the invention comprise measuring LDL and HDL subfractions in addition to Lp(a) without reference to standard risk factor measurements, such as CRP, total cholesterol, body mass index, weight, triglycerides, and the like. It is unexpected that an algorithm focusing only on LDL and HDL subfractions and Lp(a) would be more informative as to CVD risk than measurements that are much more comprehensive in terms of the markers that are reviewed. In particular, the sdLDL-C subfraction of LDL and the ApoA-1 in large alpha-1 HDL are most informative in conjunction with Lp(a).

Abstract: A method of lipid assay characterized by assaying the lipids contained in a blood component in the presence of an organic silicon compound. The method can cause specific conditions for direct methods while satisfying requirements such as no influence on precision of assay, no burden on assay apparatus, and easy availability.

Abstract: Provided is an automatic analyzer capable of replacing an insertion mechanism while suppressing occurrence of dispensing abnormality and analysis result abnormality accompanying deterioration of the insertion mechanism. Included are an insertion mechanism that is inserted into a closed container through a lid of the container; a storage unit that stores an insertion history count corresponding to a cumulative load of the insertion mechanism caused by inserting the insertion mechanism into a plurality of the containers, and an allowable insertion history count which is an allowable value of the insertion history count; and a control unit that compares the insertion history count with the allowable insertion history count and performs control to make a notification to an operator in a case where the insertion history count reaches the allowable insertion history count.

Abstract: A method for improving reliability of an integrity or leak test includes determining at least one first parameter indicative of: a first volume of a sample, a first temperature of the sample, a first pressure of the sample and/or a first assessment of integrity of the sample. The method further determines at least one second parameter indicative of a deviation from the first parameter and at least one third parameter indicative of a second assessment of the integrity of the sample. The second assessment provides an indication of a passed or failed test. The method further determines whether the deviation has an impact on the indication of the second assessment. When the deviation is determined to have an impact on the indication of the second assessment, the method comprises identifying a possibility that the indication of the second assessment is incorrect.

Abstract: Proposed is a sample transport apparatus including at least one transport line stage including first and second transport lines each configured to transport sample holders each capable of holding at least one sample along a side wall of a housing along a long-side direction, a drive mechanism configured to transport the sample holders along the first and second transport lines, and wires accommodated in the housing and connected to at least the drive mechanism, in which the first transport line and the second transport line of the transport line stage are arranged in parallel along a long-side side face of the housing, and a first distance between the first transport line and the long-side side face that is adjacent the first transport line is shorter than a second distance between the first transport line and the second transport line.

Abstract: The invention provides a small-sized automatic analyzer being compact, enabling a large number of analysis items to be carried out, and having a high processing speed. The automatic analyzer is particularly suitably applied to a medical analyzer used for qualitative/quantitative analysis of living body samples, such as urine and blood. A plurality of sample dispensing mechanism s capable of being operated independently of each other are provided to suck a sample from any one of a plurality of sample suction positions and to discharge the sucked sample to any one of a plurality of positions on a reaction disk. The automatic analyzer having a high processing capability can be thus realized without increasing the system size.

Abstract: A waveguide arrangement for measuring the speed of a projectile during passage through a weapon barrel arrangement includes a main waveguide and at least one auxiliary waveguide. The main waveguide has a cross-section suitable for the passage of the projectile. The auxiliary waveguide extends adjacent to the main waveguide and is connected to the main waveguide via an opening. An isolator is arranged in the auxiliary waveguide. The waveguide arrangement is particularly suited for large-caliber ordinance and has at least one coupler extending at least partially in the isolator. The speed of the projectile in the main waveguide is measured at a frequency below the limiting frequency. The transmitting coupler is arranged at a spacing distance from the muzzle in order to prevent an exit of the generated signal from the weapon barrel arrangement.

Abstract: A wind direction indicator for hunters includes a smoke generator, a housing, an electrical power source, an air pump and a switch all arranged such that the indicator may be actuated by a hunter using a single hand. The smoke generator may be a cartomizer as an example.

Abstract: The present invention provides a sensor with a simple structure which can precisely sense movement, etc., the sensor comprising: a head; and a support which is disposed to support one side of the head, wherein the support comprises: a first support portion for supporting the head; a second support portion which supports the head and is spaced apart from the first support portion; a first extension portion which is extended from the first support portion; a second extension portion which is extended from the second support portion; and a sensing portion which senses the deformation of the first extension portion and the second extension portion.

Abstract: A device includes two electrically isolated metalized posts attached in close proximity to each other on a bendable substrate. When the bendable substrate bends concavely with respect to the surface onto which the posts are mounted, the distance between the tops of the two posts decreases. At a fixed bending curvature, the two posts will meet and complete an electrical circuit. The posts comprise a flexible material so that the meeting of the posts has minimal effect on the spring constant or damping coefficient of the harmonic oscillation.

Abstract: A microelectromechanical systems (MEMS) accelerometer is described. The MEMS accelerometer may comprise a proof mass configured to sense accelerations in a direction parallel the plane of the proof mass, and a plurality of compensation structures. The proof mass may be connected to one or more anchors through springs. The compensation structures may be coupled to the substrate of the MEMS accelerometer through a rigid connection to respective anchors. A compensation structure may comprise at least one compensation electrode forming one or more lateral compensation capacitors. The compensation capacitor(s) may be configured to sense displacement of the anchor to which the compensation structures is connected.

Abstract: A physical quantity sensor includes: an oscillating body having a support section and a movable section which is connected to the support section through connection portions, in which the movable section has a first movable portion and a second movable portion; a first fixed electrode which is disposed to face the first movable portion; a second fixed electrode which is disposed to face the second movable portion; and a dummy electrode which is disposed to face the second movable portion so as not to overlap the second fixed electrode and has the same potential as potential of the oscillating body, in which the first fixed electrode is disposed such that a portion thereof overlaps the support section when viewed in a plan view.

Abstract: An acceleration sensor includes a base substrate provided with a first recess part, and a sensor part located on the first recess part and swingably supported in a depth direction of the first recess part by a support part, wherein the sensor part is sectioned into a first part and a second part by the support part, includes a movable electrode part in the first part and the second part, a through hole is provided at least at an end side in the second part larger in mass than the first part, and the base substrate includes a fixed electrode part in a position opposed to the movable electrode part in the first recessed part, and a second recess part deeper than the first recess part is provided in a position opposed to the end side of the sensor part.

Abstract: A method of forming a photonic inertial sensor includes providing a substrate having an insulation layer and a silicon layer on the insulation layer opposite the substrate; etching the silicon layer to form a silicon proof mass for the photonic inertial sensor; etching at least a portion of the insulation layer underneath the silicon proof mass to suspend the silicon proof mass; and depositing a high-density mass-increasing layer on the silicon proof mass to thereby increase the mass of the silicon proof mass.

Abstract: The invention relates to a device for conducting near-field optical measurements of a sample comprising a wavelength-tunable monochromatic light source. Further the invention relates to methods for measuring the near-field using such a device.

Abstract: A probe connector includes a probe body, a flexible sleeve body, a slit and a conductive fluid. The flexible sleeve body is connected to the probe body. The conductive fluid is received in the flexible sleeve body and electrically connected to the slit and the probe body. The slit is formed on one end of the flexible sleeve body opposite to the probe body, so as to define petal portions which are configured to be tightly closed together. When the slit is pressed to separate the petal portions, a portion of the conductive fluid seeps up from the flexible sleeve body via the slit.

Abstract: A multifunction testing and measuring device having a probe body with an elongated portion extending outward from a rearward main body portion to a hook-shaped forward end, the hook-shaped end useful for isolating a conductor under test, a slide closure member adapted to slide longitudinally forward from a rearward open position to a forward closed position, the closed position useful to capture the conductor under test within a space encircled by the combination of the hook-shaped end, the closed slide closure member, and the rearward main body, with the space encircled including a current sensing zone, and having test leads integral to and stowable on the main body. The multi tester comprises circuitry adapted for detecting a magnetic flux generated by an electric current passing through the conductor under test positioned within the current sensing zone, for sensing current and inrush current, as well as selectably measuring voltage, continuity, and resistance between the test leads.

Abstract: A testing probe structure for wafer level testing semiconductor IC packaged devices under test (DUT). The structure includes a substrate, through substrate vias, a bump array formed on a first surface of the substrate for engaging a probe card, and at least one probing unit on a second surface of the substrate. The probing unit includes a conductive probe pad formed on one surface of the substrate and at least one microbump interconnected to the pad. The pads are electrically coupled to the bump array through the vias. Some embodiments include a plurality of microbumps associated with the pad which are configured to engage a mating array of microbumps on the DUT. In some embodiments, the DUT may be probed by applying test signals from a probe card through the bump and microbump arrays without direct probing of the DUT microbumps.

Abstract: A metering system for metering consumption of a commodity. The metering system comprising a meter socket and a meter. The meter comprising a memory, a plurality of sensors, and a processor. Each of the plurality of sensors is configured to detect a different condition of the meter. The processor is configured to determine a plurality of events that have occurred based on the conditions detected by the sensors; compare the plurality of events to a table stored in the memory of the meter, the table defining an action to be performed by the meter for each of a plurality of different combinations of the plurality of events; select an action from the table for which the plurality of events match a corresponding combination of events stored in the table; and perform the selected action.

Abstract: In a current sensor, primary conductors each include front and back surfaces, a length direction, a width direction orthogonal or substantially orthogonal to the length direction, and a thickness direction orthogonal or substantially orthogonal to the length direction and the width direction, and further include a bent portion surrounding at least a portion of an outer periphery of a housing at an intermediate position in the length direction when viewed in the width direction. The housing is disposed in a region surrounded by the bent portion of each of the plurality of primary conductors when viewed in the width direction.