Abstract: Disclosed herein are methods of detecting Plasmodium in a subject (for example, presence of Plasmodium parasite) by detecting the presence and/or amount of one or more metabolites in a sample from the subject. In some embodiments, the methods include detecting in the sample one or more metabolites listed in Table 1, Table 2, and/or Tables 5-8. The amount of the one or more metabolites in the sample is compared to the amount of the one or more metabolites in a control and presence of Plasmodium is determined if the amount of the one or more metabolites is different (for example statistically significantly increased or decreased) compared to the control.

Abstract: The described invention comprises a method for detecting the onset or monitoring the recurrence of chronic graft versus host disease (cGVHD) in a transplantation patient. The method comprises isolating peripheral blood mononuclear cells (PBMCs) from the transplantation patient and analyzing the isolated PBMCs to detect a biomarker specific for a donor cell. The detection of the biomarker is indicative of the onset or recurrence of cGVHD in the transplantation patient.

Abstract: A method for evaluating a biological material for unassociated virus-size particles having a particular epitope uses a fluorescent antibody stain specific for binding with the epitope and a fluid sample with the virus-size particles and fluorescent antibody stain is subjected to flow cytometry with identification of fluorescent emission detection events indicative of passage through a flow cell of a flow cytometer of unassociated labeled particles of virus size including such a virus-size particle and fluorescent anti body stain.

Abstract: The present invention corresponds to the field of cancer and is related to predicting cancer detection, diagnosis, monitoring and prediction of response to treatment, in particular platelet derived-endothelial cell growth factor (PD-ECGF) levels for their use as a potential value in monitoring disease evolution and predicting response to anti-angiogenic treatment.

Abstract: The present invention is directed to claudin-1-specific peptide reagents, methods for detecting pre-cancer (dysplasia), early cancer and/or cancer using the peptide reagents, and methods for targeting pre-cancerous (dysplastic) cells, and/or cancer cells using the peptide reagents.

Abstract: The present invention relates to methods for diagnosing hematological cancers. In particular, the present invention relates to a method for diagnosing a hematological cancer in a patient comprising i) detecting the presence of CD45RARO NK cells in a sample obtained from the patient and ii) and concluding that the patient suffers from a hematological cancer when the presence of CD45RARO NK cells is detected in the sample and the presence of at least one phenotypic marker indicates the nature of the haematological cancer.

Abstract: The disclosure provides a method of predicting resistance to androgen receptor (AR) targeted therapy in a prostate cancer patient comprising (a) performing a direct analysis comprising immunofluorescent staining and morphological characterization of nucleated cells in a blood sample obtained from the patient to identify circulating tumor cells (CTCs), and (b) based on said direct analysis further determining the presence of a biomarker signature that is predictive of resistance to AR targeted therapy in the prostate cancer patient, wherein the biomarker signature comprises CK+, AR+, nucleoli+CTCs in a subpopulation of said CTCs.

Abstract: Embodiments disclosed herein provide in vitro methods for use in the culture and biochemical analysis of normal or malignant cells in autologous, allogeneic, and xenogeneic fluids and the biomarkers secreted from those cells over time and in response to various changing conditions. The methods disclosed herein provide a clinical diagnostic and research platform that provides for the study of fundamental mechanisms of cellular morphology, cellular growth kinetics, intracellular and intercellular oncogenesis, therapeutic drug mechanisms or action and efficacy, identification and use of novel biomarkers or biomarker panels.

Abstract: The present disclosure is directed to TSP1 and its coding RNA and cDNA as markers for cancer. Diagnostic methods and reagents for performing such assays also are described.

Abstract: A water-soluble peptide fluorescence material having a structure of formula (I): In formula (I), n is an integer greater than or equal to 1, R1 is independently selected from hydrogen or a nitrogen-containing functional group, R2 is independently selected from hydrogen or alkyl, and A1 is polymerized by at least one amino acid monomer and having a structure of formula (II): In formula (II), m is an integer greater than or equal to 1, and R3 in each, of the amino acid monomers of A1 is independently selected from hydrogen, alkyl, aralkyl, alkylthioaalkyl, hydroxyaralky, heteroaralkyl, carboxylalkyl, or guanidinylalkyl, A2 is —OR5 or —N(R4)2, and R4 is independently selected from hydrogen, alkyl, aralkyl, alkylthioaalkyl, hydroxyaralky, heteroaralkyl, carboxylalkyl, guanidinylalkyl, monoglycosyl, biglycosyl, or oligosaccharyl, and R5 is hydrogen, alkyl, aralkyl, alkylthioaalkyl, hydroxyaralky, heteroaralkyl, carboxylalkyl, or guanidinylalkyl.

Abstract: The present invention provides a kit for determining male fertility including a container system containing a plurality of enzyme-linked antibodies one of each capable of binding to analytes Atp6v0a2, G-CSF, MIP 1?, and MCP-1. The kit further includes suitable packaging and a set of instructions for using the enzyme-linked antibodies with a seminal sample to determine the fertility of the sample.

Abstract: A sample preparation kit related to the present invention provides a significantly versatile analytical technique that is not affected by the diversity of antibodies, difference in species, matrix and the like. For preparing a sample to be used for detection of a monoclonal antibody through high-performance liquid chromatography-mass spectrometry (LC-MS), the kit includes a porous body for immobilizing a monoclonal antibody to be detected; nanoparticles with an immobilized protease; a reaction vessel for selectively digesting the monoclonal antibody by bringing the porous body and nanoparticles into contact; a buffer to be introduced into the reaction vessel along with the nanoparticles and porous body so that a protease reaction is carried out; and a filtration membrane to remove the porous body and nanoparticles after the proteolysis so as to extract the reaction product and the buffer.

Abstract: The invention is a method for assessing protein expression by recombinant expression systems. The method uses mass spectrometry to quantify protein expression. The method has particular application in potency testing of vaccine compositions.

Abstract: Methods and sets of non-biological reagents (elution reagents, tag isomers, tag reactive groups, crosslinkers) for single or multiplexed capture and gentle elution of biomolecules. Examples are provided using amine- and cysteine-reactive reagents for enrichment of proteins, peptides, and rare peptide modifications.

Abstract: The present invention provides antibodies, or antigen-binding fragment thereof, which specifically bind to TL1A. The invention further provides a method of obtaining such antibodies and nucleic acids encoding the same. The invention further relates to compositions and therapeutic methods for use of these antibodies for the treatment and/or prevention of TL1A mediated diseases, disorders or conditions.

Abstract: The present invention is a method for determining the identity of the epitopes recognized by T-cells. The method consists of expressing an encoded library of candidate epitope sequences in a recipient reporter cell capable of providing a detectable signal upon cytotoxic attack from a single cognate T-cell followed by contacting the reporter cells with T-cells of interest. The reporter cells with a single indicating cytotoxic attack from a T-cell are isolated and then analyzed by next-generation sequencing in order to identify the epitope sequences. Specifically disclosed is a method in which a library of candidate epitope-encoding nucleic acids are expressed in cells which feature a membrane-bound major histocompatibility complex (MHC) protein, said library produced by transfection of plasmids featuring both a nucleotide encoding the candidate epitope and a nucleotide encoding a FRET-based fluorescent protein cleaved by granzyme.

Abstract: A method is provided for in vitro identification of drug-resistant epilepsy, which is based on the evaluation, in a biological sample, of the concentration of the metabolites 3-OH-butyrate, acetoacetate, choline, alanine, glutamate, scyllo-inositol, glucose, lactate and citrate.

Abstract: This invention provides for an improved method for differentially diagnosing ACTH-dependent Cushing's syndrome. Current practice for differentially diagnosing ectopic ACTH syndrome and Cushing's Disease measures relative ACTH concentrations from the inferior petrosal venous sinus compared to fluid obtained from a periphery venous sample. This is performed before and after administration of exogenous corticotropin releasing factor, or after administration of metyrapone. This invention uses glucocorticoid receptor antagonists to induce release of endogenous CRH which stimulates ACTH to increase in patients with ectopic ACTH syndrome but not in those with Cushing's Disease.

Abstract: A diagnostic method for the identification of a subject suffering from a primary non-infectious disease having an increased risk of an adverse outcome potentially being induced by the administration of an antibiotic to said subject comprising the determination of the level of Procalcitonin (PCT) or a fragment thereof or a precursor or fragment thereof having a length of at least 12 amino acid residues in a sample of a bodily fluid from said subject and the correlation of the determined level to a potential risk induced by the administration of an antibiotic, as well as a kit for performing the method and methods of treatment based thereon.

Abstract: The present invention relates to conjugate of 17-? estradiol with an analogue of indocyanine green dye for the detection of cancers. The invention also provides a method of preparation of the conjugate and method of detection of cancer cells.

Abstract: Provided is a technology useful for verifying measurement accuracy before measurement of an electrical characteristic of blood samples. A sample for use in electrical characteristic measurement is provided including erythrocytes incompletely fixed with a fixative, the fixed erythrocytes being such that an electrical characteristic of a mixture of the fixed erythrocytes and plasma components can change over time. An electrical characteristic measurement method is provided including measuring an electrical characteristic of the sample for use in electrical characteristic measurement over time before measuring an electrical characteristic of blood samples over time. An electrical characteristic measurement device is provided including a determination unit configured to determine measurement accuracy on the basis of a predetermined reference value and measured values based on the electrical characteristic measured using the sample for use in electrical characteristic measurement.

Abstract: This invention provides reagents, methods and biochemical markers for identifying and providing therapeutic intervention for individuals at risk for glucoregulatory dysfunction.

Abstract: In medical analysis devices and automatic specimen examination systems, the specimen conveyance mechanism is constituted by a plurality of belt lines. During installation or maintenance of the specimen conveyance mechanism, it is necessary to confirm the existence of steps at the joints of these belt lines and the parallelism of the conveyance line. According to the present invention, it is possible to lighten the burden of this work with a test tube type or conveyance holder type inspection device provided with a sensor and battery for operation. Also, even when an operator cannot visually confirm the conveyance line from outside, it is possible to confirm the state of the conveyance line.

Abstract: An autosampler includes: a sample cooling unit that is brought into thermally contact with a bottom surface of a sample rack so as to cool a sample accommodated in the sample rack; a condensed water receiver that has at least one hole on a bottom surface thereof, and is provided below the sample rack for receiving water condensed around the sample rack; a discharging passage configured in such a manner that a droplet falling from the at least one hole flows therein.

Abstract: Devices, systems and methods are provided for calibrating a transducer. One exemplary method involves determining a transfer function for the transducer based on a measured response of the transducer to an applied electrical signal, determining a set of values for a plurality of response parameters associated with the transducer based on the transfer function, determining a calibration coefficient value associated with the transducer based at least in part on the set of values and a correlation between physical sensitivity and the plurality of response parameters, and storing the calibration coefficient value in association with the transducer.

Abstract: An apparatus and method of performing sample characterization with an AFM and a pulsed IR laser directed at the tip of a probe of the AFM. The laser pulses are synchronized with the oscillatory drive of the AFM and may only interact with the tip/sample on selected cycles of the oscillation. Peak force tapping mode is preferred for AFM operation. Nano-mechanical and nano-spectroscopic measurements can be made with sub-50 nm, and even sub-20 nm, resolution.

Abstract: A new scanning electrochemical microscopy tip positioning method that allows topography and surface activity to be resolved independently is presented. A SECM tip is oscillated relative to the surface of interest. Changes in the oscillation amplitude, caused by the intermittent contact of the SECM tip with the surface of interest, are used to detect the surface of interest, and as a feedback signal for various types of imaging.

Abstract: A test head for a semiconductor device handler includes a plunger; and a funnel insert attached to the plunger via a funnel insert spring, the funnel insert having a channel extending axially therethrough, and the funnel insert including a plurality of sloped inner walls that are sloped outwardly in a distal direction of the funnel insert and configured to contact upper edges of a semiconductor device to center the semiconductor device in the funnel insert. The plunger includes a projecting portion that extends through the channel of the funnel insert. When the spring is in an uncompressed state, a distal end of the funnel insert extends past a distal end of the projecting portion of the plunger.

Abstract: Resistivity probes can be used to test integrated circuits. In one example, a resistivity probe has a substrate with multiple vias and multiple metal pins. Each of the metal pins is disposed in one of the vias. The metal pins extend out of the substrate. Interconnects provide an electrical connection to the metal pins. In another example, a resistivity probe has a substrate with a top surface and multiple elements extending from the substrate. Each of the elements curves from the substrate to a tip of the element such that each of the elements is non-parallel to the top surface of the substrate.

Abstract: A testing head for testing the working of a device under test comprises a plurality of contact probes, each contact probe having a rod-like body of a predetermined length that extends between a first end and a second end and being housed in respective guide holes made in at least one plate-like lower guide and one plate-like upper guide that are parallel to each other and spaced apart by a bending zone. Suitably, at least one of the lower guide and upper guide is equipped with at least one recessed portion formed at a plurality of those guide holes and realizing lowered portions thereof adapted to reduce a thickness of the plurality of those guide holes.

Abstract: A current sensor provided with an electrical coil (5) made in the form of a printed circuit. Its closed contour is inscribed within a rectangle delimiting a through window (9) for passing a primary conductor (2). It comprises four rectilinear segments (TR) connected two by two by a circular sector (SC), delimited by an interior arc (10) and an exterior arc (11). It comprises, in its circular sectors (SC), additional turns (13), which extend from exterior arc (11) towards an intermediate arc (14) located between interior arc (10) and exterior arc (11). They are inserted between the main turns (12) in such a way that the spacing between two consecutive turns (12, 13) on the exterior arc (11) is equal to spacing (P) of the main turns (12) in rectilinear segments (TR) and that the average turns density is almost constant in electrical coil (5).

Abstract: An optical voltage sensor assembly includes an input fiber-optic collimator positioned and configured to collimate input light beam from a light source. A crystal material is positioned to receive the input light beam from the light source and configured to exhibit the Pockels effect when an electric field is applied through the crystal material. An output fiber-optic collimator is positioned to receive an output light beam from the crystal material and configured to focus the output light beam from the crystal onto a detector. Methods of using the optical voltage sensor assembly are also disclosed.

Abstract: A generator waveform is measured using one or more techniques that reduce the computing resources for measuring the generator waveform and the time required for measuring the generator waveform. One example includes a template matching technique that iteratively selects templates to estimate the phase offset of the generator waveform. One example includes a root mean squared value calculated from less than sample time period that is less than one half of a cycle of the generator waveform. A generator controller calculates a command or parameter for the generator based on the estimated phase offset or the root mean squared value.

Abstract: An integrated circuit capable of on-chip jitter tolerance measurement includes a jitter generator circuit to produce a controlled amount of jitter that is injected into at least one clock signal, and a receive circuit to sample an input signal according to the at least one clock signal. The sampled data values output from the receiver are used to evaluate the integrated circuit's jitter tolerance.

Abstract: A system is disclosed. The system includes an antenna and a processor. The processor has at least four ports: a first input port coupled to a first portion of the continuity component; a first output port coupled in series to a first resistor coupled to the first potion of the antenna and to ground via a second resistor; a second output port coupled through a third resistor to the first portion of the antenna; and a second input port coupled to a second portion of the antenna and through a fourth resistor to ground. The processor is operable to activate and deactivate the appropriate ports to put the processor in one of three operating modes: an AC detection mode, an AC self-test mode, and a continuity test mode.

Abstract: A semiconductor device may include a substrate, active circuitry on the substrate and including differential transistor pairs, and threshold voltage test circuitry on the substrate. The threshold voltage test circuitry may include a pair of differential test transistors replicating the differential transistor pairs within the active circuitry, with each test transistor having a respective input and output, and at least one gain stage configured to amplify a difference between the outputs of the differential test transistors for measuring a threshold voltage thereof. The differential transistor pairs and the pair of differential test transistors may each include spaced apart source and drain regions, a channel region extending between the source and drain regions, and a gate overlying the channel region. Each of the channel regions may include a superlattice.

Abstract: A method for diagnosing an electrical circuit including at least one electrical device, an actuator for the device controlled by a high side actuating switch and a low side actuating switch, and at least one additional switch not in series with any of the HS or LS switch, the method including: to each of the possible statuses of the circuit, giving a code; sequentially putting the circuit in at least some of these statuses for a given time period; during each of these periods, measuring voltage and/or current in different parts of the circuit and giving a code to the measurement; and establishing a diagnosis of correct functioning or of a malfunctioning of at least some elements of the circuit according to a pre-established correlation between the status codes and the measurement codes.

Abstract: Embodiments herein describe the design of a scan cell within an integrated circuit. The scan cell operates in either a test mode or a normal functional mode according to a scan enable signal. The scan cell comprises delay logic including a plurality of delay elements, e.g., a plurality of transistors. The delay logic activates the delay elements only when the scan cell operates in the test mode. The delay elements are activated to change a scan latency of the scan cell. The scan latency of the scan cell is increased to mitigate or prevent hold violations.

Abstract: Embodiments herein describe the design of a scan cell within an integrated circuit. The scan cell comprises a memory element, e.g., a flip-flop, and a plurality of output buffer stages. The scan cell also comprises selection logic, e.g., a plurality of transistors. The selection logic selectively activates and deactivates one or more of the plurality of output buffer stages in response to a scan enable signal to change an output latency of the scan cell. The scan cell operates in either a test mode or a normal functional mode according to the scan enable signal. The output latency of the scan cell is changed to mitigate or prevent hold violations.

Abstract: A method for increasing a resolution of an on-chip measurement circuit is provided. The method includes propagating a first signal through the on-chip measurement circuit to generate a first output. The method also includes propagating a second signal through the on-chip measurement circuit to generate a second output. The second signal includes a delay. The method also includes reconciling the first output and the second output to determine the resolution of the on-chip measurement circuit. The resolution of the on-chip measurement circuit increases in correspondence with a fineness of a step of the delay.

Abstract: Disclosed is a method for performing reliability qualification of manufactured integrated circuit (IC) chips. In the method, IC chips are manufactured according to a design and sorted into groups, which correspond to different process windows within a process distribution for the design. Group fail rates are determined for the groups. Reliability qualification of the manufactured IC chips is performed. Specifically, a sample of the IC chips is stress tested and the manufactured IC chips are qualified if the actual fail rate of the sample is no greater than an expected fail rate. The expected fail rate used is not, however, the expected overall fail rate for all the manufactured IC chips. Instead it is a unique expected fail rate for the specific sample itself and it is determined considering fail rate contributions from only those specific groups of IC chips from which the IC chips in the sample were selected.

Abstract: Test circuits located on semiconductor die enable a tester to test a plurality of die/ICs in parallel by inputting both stimulus and response patterns to the plurality of die/ICs. The response patterns from the tester are input to the test circuits along with the output response of the die/IC to be compared. Also disclosed is the use of a response signal encoding scheme whereby the tester transmits response test commands to the test circuits, using a single signal per test circuit, to perform: (1) a compare die/IC output against an expected logic high, (2) a compare die/IC output against an expected logic low, and (3) a mask compare operation. The use of the signal encoding scheme allows functional testing of die and ICs since all response test commands (i.e. 1-3 above) required at each die/IC output can be transmitted to each die/IC output using only a single tester signal connection per die/IC output. In addition to functional testing, scan testing of die and ICs is also possible.

Abstract: The present invention provides a method to improve the JTAG debugger connection performance (TCK speed) with CPU for multi-clock designs running on Emulation or FPGA systems by creating two separate emulation or FPGA builds i.e. first build with actual frequency plan and second one with flat frequency plan (all clocks running at same frequency). The method is even effective for enabling JTAG connection to CPU in cases of designs where the running frequency of CPU is so low (due to complex clocking structure like uneven clock frequency ratios and critical paths of the design) that JTAG connection is not possible at all.

Abstract: An FPGA clock signal self-detection method relates to the technical field of control module, and the technical problem to be solved is to improve operational reliability and safety of the FPGA chip. The method comprises introducing two clock signals to an FPGA chip, wherein one clock signal is a first clock signal, and the other clock signal is a second clock signal; using the first clock signal to control all synchronous logic operations in the FPGA chip, and using the second clock signal to detect the first clock signal for correctness. The method of the invention is particularly applicable to a system with the FPGA chip as a main controller or important control unit.

Abstract: A method for making a semiconductor device may include forming active circuitry on a substrate including differential transistor pairs, and forming threshold voltage test circuitry on the substrate. The threshold voltage test circuitry may include a pair of differential test transistors replicating the differential transistor pairs within the active circuitry, with each test transistor having a respective input and output, and at least one gain stage configured to amplify a difference between the outputs of the differential test transistors for measuring a threshold voltage thereof. The differential transistor pairs and the pair of differential test transistors each includes spaced apart source and drain regions, a channel region extending between the source and drain regions, and a gate overlying the channel region. Moreover, each of the channel regions may include a superlattice.

Abstract: A method and system of determining an electro-mechanical fault condition in a synchronous machine having a stator, a rotor, and exciter. The method includes: a) obtaining an exciter current signal which is a measurement of an exciter current through stationary windings of the exciter, b) transforming the exciter current signal to obtain an exciter current frequency spectrum, and c) determining whether an electro-mechanical fault condition is present, and a type of the electro-mechanical fault condition, based on harmonic content related to the frequency of the stator, of the exciter current frequency spectrum.

Abstract: Measurement of a cell voltage is executed immediately after diagnosis of a battery management device is ended. In a battery management device, current sources repeatedly perform an energization operation to cause a current to flow to voltage detection lines with a magnitude of the current that enables each amount of charge stored in capacitors changed by one energization operation to fall within a range corresponding to a fluctuation width of terminal voltages of battery cells during the energization operation when resistors are in a normal state. When the difference between the current terminal voltage of the battery cell and the past terminal voltage of the battery cell is larger than the predetermined threshold value, the microcomputer diagnoses that the resistor is in the open state.

Abstract: A magnetic sensor includes a first magnetoresistive element that detects a magnetic field along a first detection axis, a second magnetoresistive element that detects a magnetic field along a second detection axis inclining at an angle of 45 degrees with respect to the first detection axis, a first Hall element that detects a magnetic field along a third detection axis, and a second Hall element that detects a magnetic field along a fourth detection axis perpendicular to the third detection axis. This magnetic sensor has both characteristics of the Hall elements and characteristics of the magnetoresistive elements, and has high accuracy and a small size.

Abstract: Sensor arrangement providing a signal responsive to a temperature difference between a Hall-effect device output contact and a reference point, having first contact tub located near an external surface of a Hall effect region; second contact tub located near the reference point; first conductor element comprising first and second end portions, the first end portion thermally coupled to the first contact tub and the second end portion thermally coupled to the second contact tub; second conductor element comprising third and fourth end portions, the third end portion thermally coupled to the first contact tub; third conductor element comprising fifth and sixth end portions, the fifth end portion thermally coupled to the second contact tub, wherein the first and third end portions are electrically coupled, the second and fifth end portions are electrically coupled, at least two of first, second, and third conductor elements have substantially different Seebeck coefficients, and the signal is tapped at the fourth

Abstract: Some implementations provide a method for safe operation of a magnetic resonance imaging (MRI) system, the method including: determining, at least in part by using a sensor device, location information that indicates a location of an MR-incompatible object relative to the MRI system, the MRI system generating a polarizing magnetic field for imaging a subject; based on the determined location information, determining, by a control unit associated with the MRI system, that the MR-incompatible object poses an operational hazard to the MRI system; and in response to determining that the MR-incompatible object poses an operational hazard to the MRI system, reducing, by the control unit, a strength of the polarizing magnetic field.