Abstract: The invention relates to a method of analysing a sample, comprising the analysis of clinical-chemical parameters and the analysis of immunodiagnostic parameters in a fully automatic analysis apparatus, wherein the analysis apparatus comprises a pipetting apparatus, at least one holder for a reagent cartridge containing the components necessary for carrying out the analysis, a holding apparatus for a measurement cell, at least one measurement cell, with each reagent cartridge being associated with a measurement cell, a holding apparatus for a sample container containing sample, and a photometric or spectrometric measurement device. On the basis of a sample it is possible here for a plurality of different clinical-chemical and/or immunodiagnostic parameters to be determined, to which end a dedicated reagent cartridge is inserted into a holding apparatus of the analysis apparatus for each clinical-chemical or immunodiagnostic parameter to be determined.

Abstract: Provided herein are methods of diagnosing or monitoring the treatment of abnormal glycan accumulation or a disorder associated with abnormal glycan accumulation.

Abstract: The present invention relates to an in vitro method for detecting cAMP or cGMP comprising a) contacting a mixture with a complex of a tracer and a dequencher, wherein the tracer is a fluorophore covalently linked to a cAMP quencher, and b) measuring the change in fluorescence. Furthermore, the present invention relates to the use of said method for determining the cAMP or cGMP concentration in the mixture, for determining the activity of a receptor wherein the signal transduction of the receptor comprises cAMP or cGMP and for screening of a ligand for such a receptor.

Abstract: A new signal amplification method exploiting the dense atom packing in metallic nanocrystals has been developed for detecting target substances. By dissolving nanocrystals to individual ions that are stoichiometrically converted to chromophores and quantified photometrically, extremely high signal amplification can be achieved. Signal amplification is fully determined by the total number of atoms in the nanocrystals bound to a single target molecule. The disclosed nanocrystal amplification method can be implemented with a rich selection of metal/metal oxide nanocrystals and metal-reactive chromogenic substrates. The chromogenic reactions can be either solution-based or surface-based and performed in aqueous or organic phase, supporting a variety of assay formats.

Abstract: Method of localizing a sulfhydryl (—SH) group containing biomolecule to the surface of a cell membrane by mixing in a volatile reaction buffer a molar excess of the sulfhydryl (—SH) group containing biomolecule with a lipid conjugated maleimide of the structure F-S-L as defined in the specification to provide a reaction mix, incubating the reaction mix for a time and at a temperature sufficient to allow all the lipid conjugated maleimide to have reacted with the sulfhydryl (—SH) group, and freeze-drying the reaction mix to remove the volatile reaction buffer and provide a reaction product. An aqueous solution of the reaction product is contacted with the cell membrane.

Abstract: A chip for localized surface plasmon resonance (LSPR) biosensing and imaging having a glass coverslip compatible for use in a standard microscope and at least one array of functionalized plasmonic nanostructures patterned onto the glass coverslip with electron beam nanolithography. The nanostructures can be regenerated allowing the chip to be used multiple times. Also disclosed is a method for determining the fractional occupancy values for surface-bound receptors as a function of time for LSPR biosensing from the spectroscopic response of the array and modeling the photon count in each spectrometer channel, allowing for a functional relationship to be determined between the acquired spectrum and the fractional occupancy of binding sites on the array. Additionally disclosed is a method for the spatiotemporal mapping of receptor-ligand binding kinetics in LSPR imaging using the chip and projecting a magnified image of the array to a CCD camera and monitoring the binding kinetics of the array.

Abstract: The present invention relates to a method for determining whether a protein binds to a specific DNA sequence. This method is useful in particular for identifying modifications to the DNA sequence (e.g. methylations) via the binding of proteins that specifically recognize those modifications (e.g. antibodies), but also to identify the binding sequence on DNA of a variety of proteins.

Abstract: A hand-held measuring instrument is used for detecting hidden mold damage in interior spaces. The measuring instrument includes at least one collection unit configured to intake at least of one interior space sample, at least one buffer unit, at least one immunological test unit, an evaluation unit and a control panel. The at least one buffer unit has a first compartment and a second compartment.

Abstract: Fungal infections are difficult to diagnose. The most common filamentous fungal infection, aspergillosis, carries with it a high mortality. Culture of the organism is difficult and obtaining samples, e.g., through a lung biopsy, sometimes causes morbidity. Biomarkers that indicate ‘early’ infection in it development are sought after. One such biomarker is detection of galactomannan (GM), a polysaccharide that is attached to hyphal cell walls and secreted during growth of the organism. Galactomannan is excreted in urine. Disclosed herein is a lateral flow assay comprising monoclonal antibodies that recognize specific residues of Aspergillus fumigatus for detecting GM in urine samples to provide a point-of-care detection device to allow for frequent screening and early diagnosis in patients at high risk for infection.

Abstract: The present invention is directed to combination immunoassays, reagents and kits for simultaneous detection of HCV antigens and anti-HCV antibodies in a sample. The combination immunoassays of the present invention employ a non-ionic detergent that effectively exposes or releases the HCV core antigen from virions in a sample without interfering with the performance of other reagents such as the capture of anti-HCV antibodies by recombinant HCV antigens.

Abstract: The present invention describes methods of prognosticating patient survival based on an analysis of the ZEB1 gene or gene product. The present invention also describes methods of selecting and/or selecting various therapies based on the analysis of ZEB1, IDH1, PTEN, MGMT and/or RET. Further describes are systems for analyzing ZEB1, IDH1, PTEN, MGMT and/or RET as well as selecting and/or selecting the therapies.

Abstract: Provided herein are methods for determining the risk that a subject diagnosed with colorectal cancer will develop a recurrence of colorectal cancer and methods of predicting clinical outcome for a subject diagnosed with colorectal cancer by a) determining the level of expression for each marker of a panel of markers in a panel of tumor compartments in a tumor tissue sample from the subject, wherein the panel of markers comprises at least two of TEM1, HIF2?, CAIX, PDGFR?, fibronectin, collagen I, collagen IV, and CD31 and wherein the panel of tumor compartments comprises at least three tumor compartments of pure stroma, tumor, stromal vessel, and tumor vessel; b) determining the TAPPS score for said subject; and c) comparing the TAPPS score of the subject to the TAPPS score of a population of subjects diagnosed with colorectal cancer. Also provided are related computer-implemented methods and systems, kits, and tumor microarrays.

Abstract: Provided are a composition, a kit, and a method of predicting prognosis of ovarian cancer or a risk of recurrence of ovarian cancer. Provided are a composition for treating ovarian cancer or preventing recurrence of ovarian cancer and a method of screening a material for treating ovarian cancer or preventing recurrence of ovarian cancer. According to the present disclosure, prognosis or recurrence of ovarian cancer can be efficiently diagnosed, and a candidate material that can treat ovarian cancer or prevent recurrence of ovarian cancer can be efficiently screened.

Abstract: The present invention concerns materials and methods for characterizing monoclonal immunoglobulin specificity of a Monoclonal Gammopathy of Undetermined Significance (MGUS) or Myeloma patients using a protein microarray comprising (a) a substrate, (b) antigens immobilized on the substrate, said antigens being selected from a defined group consisting of infectious agent antigens and/or self-antigens. In particular said protein microarray may be used to improve diagnosis, for the prognosis of myeloma or MGUS, for preventing transformation of MGUS toward myeloma, for adapting treatment of MGUS and myeloma or for monitoring the response to therapy of MGUS and myeloma patients.

Abstract: Provided are methods for determining the amount of thyroglobulin in a sample using various purification steps followed by mass spectrometry. The methods generally involve purifying thyroglobulin in a test sample, digesting thyroglobulin to form peptide T129, purifying peptide T129, ionizing peptide T129, detecting the amount of peptide T129 ion generated, and relating the amount of peptide T129 ion to the amount of thyroglobulin originally present in the sample.

Abstract: The present invention relates generally to methods for diagnosing the presence or the risk of development or the therapy control of spondyloarthritis (Spa), in particular, of ankylosing spondylitis (AS) and undifferentiated spondyloarthritis in a subject, in particular in mammals. In addition, the present invention relates to test kits for use in the diagnosis of the presence or the risk of development, or for the therapy control of Spa, like AS and undifferentiated spondyloarthritis, in a subject. In particular, the present invention relates to a method for diagnosing the presence or the risk of development, or for the therapy control of Spa, like AS and undifferentiated spondyloarthritis, in a subject analysing for the presence of autoantibodies against CD74 and/or IKBKB in a subject. The presence of autoantibodies against CD74 and/or IKBKB is indicative for the presence or the risk of development, or for the therapy control of Spa, like AS and undifferentiated spondyloarthritis.

Abstract: System and methods are disclosed for identifying and isolating antibodies with specific affinity with an antigen of interest. Multiple DNA libraries encoding antibodies or their fragments are designed such that the encoded antibodies from different libraries are tagged differently. These libraries may be transformed into yeast cells. The variants of the antibodies are displayed on the surface of the yeast cells and flow cytometry may be used to sort the cells based on antigen affinity and the different tags on the antibodies. By allowing multiple libraries to be screened simultaneously, the disclosed methods help improve the efficiency of affinity.

Abstract: The application discloses MCAM as a new biomarker for fluid homeostatic imbalance; methods for predicting, diagnosing, prognosticating and/or monitoring fluid homeostatic imbalance based on measuring said biomarker; and kits and devices for measuring said biomarker and/or performing said methods.

Abstract: Provided herein are methods for diagnosing the presence or the risk of development, or for the therapy control of vasculitis, in particular of large vessel vasculitis, like giant-cell arteritis (GCA), polymyalgia rheumatica (PMR), and Takayasu's arteritis, in a subject analyzing for the presence of antibodies against ferritin, in particular heavy chain ferritin or immunoreactive peptides thereof or ferritin analog protein, preferably bacterial ferritin analog protein, or immunoreactive peptides thereof, in a subject. In addition, test kits for use in the diagnosis of the presence or the risk of development, or for the therapy control of vasculitis, in particular of large vessel vasculitis, like GCA, PMR and Takayasu's arteritis, in a subject are also provided.

Abstract: The invention relates to antibodies, antibody fragments and binding agents that specifically recognize oligomeric tau but do not bind to monomeric tau, fibrillar tau or non-disease associated forms of tau.

Abstract: The present invention provide water soluble polyfluorenes functionalized with glucuronic acid useful in sensing bilirubin in aqueous medium and process for preparation thereof. The invention further deals with detecting bilirubin in human serum samples in the range from normal (<25 ?mol/L˜1.2 mg/dL) human bilirubin level to jaundiced bilirubin level (>50 ?mol/L˜2.5 mg/dL).1 This is a fluorescence turn-off mode of detection where blue fluorescence of polymer quenches and becomes colorless. The water soluble polyfluorenes functionalized with glucuronic acid can detect free bilirubin in the range from 1×10?4 M to 1×10?7 M moles in aqueous and buffer media as a change in the fluorescence signal.

Abstract: A method of detecting one or more analytes comprising or consisting of hydrogen peroxide using surface enhanced Raman spectroscopy (SERS) is provided. The method includes providing a SERS-active substrate having at least one metal carbonyl cluster compound attached thereon; contacting one or more analytes with the SERS-active substrate; and detecting changes in surface enhanced Raman signal from the at least one metal carbonyl cluster compound as an indication of the presence of one or more analytes comprising or consisting of hydrogen peroxide.

Abstract: The present invention is directed to a cartridge device for a measuring system for measuring viscoelastic characteristics of a sample liquid, in particular a blood sample, comprising a cartridge body having at least one measurement cavity formed therein and having at least one probe element arranged in said at least one measurement cavity for performing a test on said sample liquid; and a cover being attachable on said cartridge body; wherein said cover covers at least partially said at least one measurement cavity and forms a retaining element for retaining said probe element in a predetermined position within said at least one measurement cavity. The invention is directed to a measurement system and a method for measuring viscoelastic characteristics of a sample liquid.

Abstract: According to an embodiment of the disclosure, the analyzer includes a reagent driving disk that accommodates a reagent configured for analysis and that transports the reagent to a desired position, and a fixed disk that has a reagent stand-by position in which to make a reagent container containing the reagent, temporarily stand by, and a magnetic particles stirring position for stirring magnetic particles. A portion of the reagent stand-by position is constituted by a loading system. A reagent container moving unit moves reagent containers containing the reagent, between the reagent driving unit and the fixed disk, according to analytical request status. Providing in a part of the fixed disk the loading system constructed so that reagent containers containing the reagent can be mounted therein during operation enables changing of reagent containers, irrespective of an operational status of the reagent driving disk, and the system to having cold-storage functionality.

Abstract: A tube rack transfer device for transferring racks is presented. A first rail extends in a first horizontal direction and a second rail extends in a second horizontal direction orthogonal to the first direction. The second rail moves along the first rail and comprising a transfer head movable along the second rail. The transfer head comprises a control pin to be coupled with one of: an input pusher, translatable in the second direction, for transferring a rack from a carrier to a sampling area of an analyzer; an output pusher for transferring a rack from the sampling area to a carrier; a rack for transferring the rack between different carriers and/or between different positions of the same carrier. An in-vitro diagnostic instrument comprises an analyzer for carrying out tests on biological samples, a sample unit for inputting/outputting racks, a sampling area for withdrawing samples from tubes, and a transfer device.

Abstract: An aspiration apparatus adapted to allow aspiration verification of a liquid such as of a biological liquid or reagent liquid, especially at low aspiration volumes (e.g., less than 25 ?L). The apparatus includes a pump operable at an operating frequency, the pump optionally including a pumping chamber, a probe having a probe interior, a main supply line containing a backing liquid coupled to and extending from the pump and including the probe interior, a striction coupled to the main supply line, or the pump chamber, if present, and a reservoir fluidly coupled to the striction, wherein the striction is sized to minimize backing liquid flow through the striction at one or more disturbance frequencies above the operating frequency. Systems and methods for carrying out the liquid aspiration are provided, as are other aspects.

Abstract: Methods of determining or verifying proper operation of a diagnostic device are disclosed. The methods include monitoring, with one or more image capture devices, one or more locations to determine proper aspiration, dispense, and/or overall assay component volume fill of one or more bio-liquid containers. Tip immersion depth and location may be also measured and/or verified. Diagnostic devices and apparatus adapted to carry out the method are described, as are other aspects.

Abstract: A method for receiving a sound generated from a moving subject and an electronic device implementing the same are provided. The method includes confirming a position of a subject so as to intensively receive a sound generated from the subject, photographing a moving image, selecting at least one subject among subjects included in the photographed moving image, obtaining movement information of the selected subject, and adjusting a major sound capture region based on the obtained movement information of the subject.

Abstract: A data acquisition method of a substrate processing apparatus for acquiring data on gas current directions in a plurality of measurement regions on a surface of a substrate, includes: loading a sensing substrate having a plurality of pairs of sensors on a loader, wherein each pair of sensors includes a first sensor and a second sensor configured to acquire vector data of the gas current on the surface of the sensing substrate; acquiring vector data of the gas current in a first linear direction by the first sensor; acquiring vector data of the gas current in a second linear direction slanted to the first linear direction by the second sensor; and combining the gas current vectors based on a starting point associated with the respective pair of sensors; and calculating a gas current direction from the starting point associated with the respective pair of sensors.

Abstract: A sensor device for an electronic apparatus is provided with: a sensing structure generating a first detection signal; and a dedicated integrated circuit, connected to the sensing structure, detecting, as a function of the first detection signal, a first event associated to the electronic apparatus and generating a first interrupt signal upon detection of the first event. The dedicated integrated circuit detects the first event as a function of a temporal evolution of the first detection signal, and in particular as a function of values assumed by the first detection signal within one or more successive time windows, and of a relation between these values.

Abstract: A method of detecting whether a microelectromechanical system (MEMS) device is hermetic includes applying at least three voltage differences between a movable part and a sensor electrode of the MEMS device to measure at least three effective capacitances, calculating a capacitance-to-voltage curve and an offset voltage of the MEMS device according to the at least three effective capacitances; and determining whether the offset voltage is within a predetermined range to determine whether MEMS device is hermetic.

Abstract: A faulted circuit indicator (FCI) device for installation on a power line includes a housing, a laminate structure fastened to the housing and configured to receive the power line, and a clamp mechanism pivotally fastened to the housing and configured to secure the housing to the power line. The clamp mechanism includes a pair of opposing clamp arms each having a curved configuration. The curved configuration of the clamp arms maintains the power line in a centered relationship within the laminate structure when the clamp arms are engaged on the power line over a range of power line diameters.

Abstract: A semiconductor test apparatus includes a pogo pin that is provided on a board and is in contact with an inspected object. The pogo pin includes a barrel fixedly disposed on the board, a plunger movably coupled to the barrel, and a conduction film covering the pogo pin. The conduction film contacts a portion of the plunger to be electrically connected to the plunger while being electrically insulated from the barrel.

Abstract: A structure and method of facilitating testing of an electronic device (device under test or DUT) using a non-permanent and reusable structure to terminate contact pads and contact pin holes on a surface of the DUT.

Abstract: Electrical connection device comprising at least one substrate and one or several first electrical connection elements located on a front face of the electrical connection device such that they can be coupled to contact pads of an electronic device to which the electrical connection device is intended to be connected, each first electrical connection element comprising: at least one support, of which at least one first end is anchored to the substrate such that part of the support is suspended above the front face, the support comprising at least a portion of piezoelectric material located between two electrodes and capable of moving said part of the support in two directions approximately perpendicular to the front face depending on a value of an electrical voltage intended to be applied onto the electrodes; at least one electrical conducting element located on said part of the support.

Abstract: A sensor detects the rotation of a component (object) of a vehicle in a non-contact manner, and generates a differential signal according to the rotation. Two comparators have different hysteresis characteristics. At both edges of one signal that is an output of one of the comparators, if a signal of the other comparator is not at the same level, it is determined that a distance (gap) between an object and the sensor is within a predetermined range in which the differential signal is effective.

Abstract: A current detection system includes: first and second magnetic plates arranged in parallel with a predetermined distance; a bus bar for flowing current; and a magneto-electric conversion element converting a lateral direction component of a measurement object magnetic flux, generated by current flowing through the bus bar, to an electric signal. When an external magnetic flux in a lateral direction passes through an accommodation space between the first and second magnetic plates, a trajectory of the external magnetic flux is bent by the first and second magnetic plates, and the accommodation space is divided into a permeable space, through which the external magnetic flux passes, and a non-permeable space, through which the external magnetic flux does not pass. The bus bar is arranged in the accommodation space. The magneto-electric conversion element is arranged in the non-permeable space. The bus bar and the magneto-electric conversion element are lined in the height direction.

Abstract: A voltage sensor housing includes a top portion including a conductive top portion composed of conductive material and non-conductive top portions composed of non-conductive material, a bottom portion composed of non-conductive material, side portions composed of non-conductive material, wherein the top portion the bottom portion and the side portions define an interior area structured to hold a voltage sensor, and conductive side portions composed of conductive material and being disposed adjacent to the side portions. The conductive top portion is electrically floating and the conductive side portions are electrically grounded.

Abstract: A real current meter reads current from a current probe coupled around power lines of a transformer-based UPS system coupled to a transformer having a high resistance ground with a HRG resistance and determines a real current component of the current read from the current probe.

Abstract: A meter reading system for communicating with Automated Meter Reading meters. The system includes an airborne platform and a meter data collection system disposed on the airborne platform. The meter data collection system includes antennas, with each of the antennas pointing in a different direction, one from another, and each antenna generally pointing downward when the airborne platform is airborne. The antennas receive meter data from the meters. The meter data collection system also includes transceivers, one each of the transceivers associated with and in signal communication with one each of the antennas. The transceivers receive the meter data through the antennas. A first computer is in signal communication with the transceivers, and receives the meter data from the transceivers.

Abstract: A measurement device has a sensor that generates current by electromagnetic coupling to a power line, a measurement circuit that measures one of voltage and power, the voltage and the power being obtained by the current generated in the sensor according to voltage at the power line, a conductive member electrically connected to a signal ground line of the measurement circuit, and an insulating member that brings the conductive member close to an external conductor and insulates the conductive member from the external conductor.

Abstract: A method for ascertaining a load capacitance of a data bus includes reading in and a determining task. In the reading in, a scaling factor of a synchronization pulse of the data bus is read in. In the determining, the load capacitance is determined using the scaling factor and a processing rule.

Abstract: In an embodiment, a missing utility ground detection circuit includes a pair of balanced resistors each connected to receive utility voltage from a different one of a pair of utility power lines, the balanced resistors being connected together at a summing node to be capable of summing the voltages from the pair of utility power lines. It includes an unbalance resistor connected to shunt voltage from one of the utility power lines. It has a summing amplifier with an input coupled to the summing node and to a reference voltage, and an input coupled to a second reference voltage. It has an averaging circuit connected at the output of the summing amplifier. A comparator is provided having an input connected to the averaging circuit an input connected to a threshold voltage.

Abstract: A system and method for diagnosing insulation-breakdown of a vehicle component including a system in a vehicle including high voltage components are provided. The system includes a battery pack that is configured to supply a high voltage to the vehicle and a high voltage relay that is connected to an output stage of the battery pack. Additionally, a high voltage component controller is connected to an output stage of the high voltage relay to turn the high voltage component on and off. A battery management system interlocks with the battery pack, the high voltage relay, and the high voltage component controller to diagnose whether the battery pack, an input stage of the high voltage component controller, and the high voltage component are insulated-breakdown.

Abstract: A method for detecting electrical faults in an electrical circuit having transmission wires for power transmission includes determining whether a potential electrical fault condition exists along the transmission wires and confirming the potential electrical fault is an actual electrical fault. If the potential electrical fault is confirmed as an actual electrical fault, the method may disable the electrical circuit.

Abstract: Examples provided herein describe a female connector in a computing device that includes a plurality of sensors for determining if a male connector has established a proper connection with the female connector. To do so, in one example, each of the sensors is coupled to an actuator that protrudes into an aperture defined by an inner surface of the female connector. As the male connector is inserted into this aperture, the actuators are pressed down which activates the sensors. Furthermore, the actuators are arranged such that a first actuator is deeper within the aperture than a second actuator. Thus, if the male connector pressed down the first actuator but not the second, a computing device can determine that only a partially connection was made. By using at least two actuators arranged at different depths in the aperture, the computing device is able to detect a proper or improper connection.

Abstract: Techniques for adaptively scaling power supply voltage of a programmable integrated circuit. Compact speed-testing ring oscillators are inserted into a pre-constructed circuit model to test the speed of speed-critical aspects of the interconnect fabric of the programmable integrated circuit. The speed-testing ring oscillators are compact due to including only two elements configured from lookup table elements (“LUTs”) of the programmable integrated circuit. The speed-testing ring oscillators are connected to a power management unit which receives speed values output from the speed-testing ring oscillators and adjusts the power supply voltage to maintain the speed-testing ring oscillators operating at or above a prescribed speed. If all speed-testing ring oscillators are operating too fast, then power management unit reduces voltage to reduce the total power consumed by the programmable integrated circuit while still maintaining operation above a desired speed.

Abstract: A computer-implemented method includes, by one or more processors in electronic communication with a tunneling magnetoresistive sensor, wherein the tunneling magnetoresistive sensor is a component of a magnetic storage drive configured to read magnetic data from a magnetic storage medium, detecting a short across the tunneling magnetoresistive sensor, measuring a change in resistance of the tunneling magnetoresistive sensor, measuring a change in voltage amplitude for the tunneling magnetoresistive sensor, and dividing said change in voltage amplitude by said change in resistance to yield a ratio. The computer-implemented method further includes, responsive to the ratio being greater than a predetermined ratio threshold, determining that the short is caused by a magnetic shunt. A corresponding computer program product and computer system are also disclosed.

Abstract: A device of contacting a substrate with a probe card includes a mounting table 15 that transfers a wafer W together with a wafer plate 24 to a position facing the probe card 19; a lifting device 15a that contacts multiple electrodes of semiconductor devices formed on the wafer W with multiple probes of the probe card 19 by moving the wafer plate 24 and the wafer W toward the probe card 19 and then further moves the wafer W toward the probe card 19; a depressurization path 26 that decompresses a space S between the probe card 19 and the wafer plate 24 and maintains a contact state between the electrodes of the semiconductor devices and probes 19b of the probe card 19; and the lifting device 15a that separates a chuck member 14 on the mounting table 15 from the wafer plate 24.

Abstract: A method of probe testing dies, the method includes loading a wafer having a first die and a second die into a prober and bringing probes of the prober into contact with first contact pads of the first die according to first probe parameters. A first probe contact test of first values of the contact between the probes and the first contact pads is performed, and a die test of the first die is performed after performing the probe contact test. Results of the die test and results of the probe contact test are saved and second probe parameters are automatically generated based on at least the results of the first probe contact test.