Abstract: Electrotransfer is performed in an instrument that receives electroblotting cassettes and that contains an integrated power supply, controls, and a display that allows the user to monitor and control each of a plurality of cassettes individually through electrical contacts within the housing that mate with corresponding electrical contacts on the cassettes.

Abstract: Kits, systems, and methods are provided for transferring biological macromolecules from an electrophoresis slab gel to a blotting membrane using conductive polymer electrodes.

Abstract: An integrated system for performing electro-blotting, probing and drying of the membrane is disclosed. The integrated system comprises a transfer unit for receiving one or more transfer sandwich holder. Each transfer sandwich holder holding a transfer sandwich comprises a gel member and the membrane. The transfer unit is configured to transfer samples from the gel member to the membrane. The integrated system also includes a probing unit for receiving the membrane therewithin. The membrane is exposed to a plurality of antibodies for binding with the samples in the membrane. A drying unit is also present for drying the membrane with hot air.

Abstract: A method of detecting chlorate in soil includes contacting soil wetted with a solvent containing an electrically conductive salt with an electrode comprising layers of vanadium-substituted phosphomolybdate alternating with layers of para-rosaniline, and performing voltammetry with the electrode, wherein a catalytic reduction current indicates a likelihood of the presence or absence of chlorate in the soil. A system includes a potentiostat operably connected to the electrode and in communication with hardware and software sufficient to produce an output indicating a chlorate level in soil.

Abstract: A system and method is provided for simultaneous ion current sensing and gas analysis. The system acquires an ion current signal and analyzes the composition of a corresponding gas sample.

Abstract: The present application is directed to a process for calibrating a detection apparatus, especially an ion mobility spectrometer, using isoflurane (CAS Reg. No. 26675-46-7) as a chemical standard whereby calibrating the detection apparatus for a known target chemical is based on an evaluation of the experimental data collected for the negative isoflurane monomer ion against the experimental data collected for the negative isoflurane dimer ion.

Abstract: The invention relates to improved systems and methods for inspecting the tubes of a steam generator of a nuclear reactor that involves modeling the steam generator, comparing signals of a tube from an eddy current sensor with aspects of the model to determine whether further analysis is required, employing primary and secondary analysis processes, and producing a combined report of the primary and secondary analysis results.

Abstract: A method for testing a fuel cell stack includes providing a fluid, such as an ammonia-containing fluid, in a first reactant flow path in a first portion of the fuel cell stack, detecting the presence of the fluid using a detector, such as an ammonia detector, positioned within or adjacent to a second portion of the fuel cell stack that is separated from the first portion of the fuel cell stack and determining the presence of a defect in the stack based on detecting the presence of the fluid. Further embodiments relate to testing a fuel cell stack using a microphone that detects an audio signal indicative of a stack defect.

Abstract: The invention relates to an ultrasonic NDT sensor arrangement for inspecting surfaces of variable geometry of metal bodies. The sensor arrangement includes a housing with a hollow inner space and an opening, through which part of an incoming sound beam exits the housing to enter a metal body to be tested. An ultrasonic sensor is coupled to the housing such that it emits said incoming sound beam directly into said hollow inner space in a direction towards the opening. The said inner space of the housing is filled with a first coupling fluid which is water. The opening of the housing is closed with a closing member, which is made of a silicone material and which has a similar density and velocity of sound compared to the first coupling fluid.

Abstract: A phased array transducer for inspecting a fastener hole and adjacent structure to identify defects and determine hole integrity without removing the fastener from the hole. The phased array transducer includes a plurality of transducer elements, where one of the transducer elements is used to align the transducer to the hole, one group of the remaining transducer elements inspects the entire thickness of the structure at one side of the fastener and another group of the remaining transducer elements inspects the entire thickness of the structure at an opposite side of the fastener.

Abstract: Embodiments of an ultrasonic button and methods for using the ultrasonic button are disclosed. In one embodiment, an ultrasonic button may include an ultrasonic transmitter configured to transmit an ultrasonic wave, a piezoelectric receiver layer configured to receive a reflected wave of the ultrasonic wave, a platen layer configured to protect the ultrasonic transmitter and the piezoelectric receiver layer, a first matching layer configured to match an acoustic impedance of the platen layer with an acoustic impedance of ridges of a finger, and an ultrasonic sensor array configured to detect the finger using the reflected wave.

Abstract: A transducer assembly includes an acoustic sensor element and an acoustic waveguide. The acoustic waveguide includes a rotatable acoustic coupler, a tube, and a foot. The foot has a mounting surface that is mountable on a fluid conduit. A circuit assembly couples to acoustic sensor element and provides a diagnostic output.

Abstract: In a liquid chromatography system, a sample is injected into a column by flowing a solvent mixture from a mixer into the column along a solvent mixture flow path; and injecting a sample into the solvent mixture flow path downstream of the mixer. In another liquid chromatography system, a sample is injected into a column by loading an isolator fluid into a sample loop, loading a sample into the sample loop, and flowing the sample into the column as a plug in front of the isolator fluid.

Abstract: Composite sampling of a fluid flowing through a conduit includes collecting, in a vessel coupled to the conduit through which the fluid is flowing, a first discrete sample of fluid from the conduit, the first discrete sample having a first selected volume, and collecting, in the vessel and at a first interval from the first sample, a second discrete sample of the fluid from the conduit, the second discrete sample having a second selected volume, thereby forming a composite sample in the vessel while the vessel is coupled to conduit. The composite sample includes the first discrete sample and the second discrete sample, and may include one or more additional discrete samples. An apparatus for collecting the composite sample includes a gas chromatograph, and is arranged such that the composite sample is provided to the gas chromatograph without removing the composite sample from the apparatus or transporting the composite sample.

Abstract: The invention relates to a measurement system including a network of nanoelectromechanical system (NEMS) resonators, characterized in that: each one of said resonators includes: an electrostatic activation device capable of generating a vibration of a beam exposed to said excitation signal, at least one piezoresistive stress gauge made of a doped semiconducting material, extending from the beam so as to detect a movement of said beam, the variation in the electrical resistance of said at least one gauge supplying an output signal; said network includes at least two groups of resonators, each group including at least two resonators having an identical empty resonance frequency, each group of resonators having an empty resonance frequency different from that of each other group; the resonators forming each group are connected in parallel; the groups of resonators forming said network are connected in parallel; said system includes a reading device designed to supply an excitation signal at the network input and

Abstract: With a device for combustion analysis, comprising an induction furnace with a furnace chamber, in which carrier gas can flow during operation via at least one gas inlet to a gas outlet, and in which a sample to be analyzed can be arranged and burned in a sample container, a hollow protective element is provided and, with normal operation of the device, is arranged in the furnace chamber directly above the sample in such a way that the end of the protective element facing towards the sample, together with the sample container, forms a constriction for the carrier gas flow, wherein the protective element is desgned to convey gases produced during the combustion of the sample through the protective element and to the gas outlet.

Abstract: Nanofiber-based sensors for the rapid detection, identification, and/or quantification of analytes, including gaseous analytes such as oxygen, are provided. The nanofiber-based sensors can comprise core-shell nanofibers. The core-shell nanofibers can comprise (a) a core comprising a first polymer and an sensor dispersed therein; and (b) a shell disposed coaxially around the core, comprising a second polymer.

Abstract: Predictive analysis of complex datasets and systems and methods including the same are disclosed herein. The methods include analyzing airborne particle count data from a cleanroom environment to predict a particle count fault condition within the cleanroom environment. The methods further include generating an airborne particle count data repository that includes particle counts within the cleanroom environment, analyzing the airborne particle count data to calculate a difference between a first rate of change and a second rate of change, and predicting the particle count fault condition responsive to the difference between the first rate of change and the second rate of change being outside a predetermined threshold range difference. The systems include computer readable storage media including computer-executable instructions that, when executed, direct a data analysis system to perform the methods.

Abstract: A gas sensor which works according to the principle of thermal conductivity is functionally tested. In the method, a calibration cycle is conducted in which a membrane of the gas sensor is immersed in a fluid calibration medium having a known concentration of a target gas. After the calibration cycle, a measurement chamber of the gas sensor is purged with a purging gas. Then, a measuring cycle is conducted, using a thermal conductivity sensor to measure the target gas in the measurement chamber. Using a calibration baseline established from the calibration cycle and a measurement baseline in the measurement cycle, a baseline comparison value is obtained and compared to a predetermined baseline threshold value. An error message, indicating a malfunction in the purging gas supply, is generated when the baseline comparison value exceeds the predetermined baseline threshold value.

Abstract: A method for testing a gas sensor is based on a gas-measuring system with a test gas source and with a pumping device. A predefined quantity of a gas or of a gas mixture is fed in, from the test gas source, to the gas sensor over a predefined time and metered. The response of a measured signal of the gas sensor is determined as a sensor response. Characteristic variables, from which an indicator of the ability of the gas sensor to operate is determined, are determined from the sensor response.

Abstract: A self-contained UAV sensor payload and on-board or off-board hardware and software for precision agriculture. The invention combines multiple cameras and navigation sensors to create a system for automatically collecting, geo-referencing, and stitching red, green, blue, and near infrared imagery and derived vegetation indices. The invention is able to produce all of these quantities in a single flight. The tight integration with integrated navigation sensors eliminates the need for integration with external sensors or avionics hardware and enables innovative solutions to image processing and analysis which greatly improve accuracy, processing speed, and reliability over alternative approaches.

Abstract: According to the invention for a deep-frying device (2) including a stationary deep-frying oil sensor (5) which immerses into deep-frying oil (4) of a deep-frying oil basin (3), in order to monitor the stationary deep-frying oil sensor (5), a receiving unit (17) having a receiving data interface (8) is provided, via which interface a reference value obtained beforehand by a portable deep-frying oil measuring device (14), the portable deep-frying oil sensor (15) of which is immersed into the deep-frying oil (4) in the deep-frying oil basin (3) for measuring, can be transmitted in order to adapt a characteristic curve stored in the stationary evaluation unit (6) of the deep-frying device (2) such that the stationary evaluation unit (6) calculates a value for an evaluation variable for characterizing the deep-frying oil (4), this value being consistent with the reference value of the portable deep-frying oil measuring device (14).

Abstract: A calibration solution for a wet chemistry analyzer is provided. The solution comprises a calibration agent. The solution also comprises a cleaning agent. The cleaning agent is selected such that the wet chemistry analyzer is simultaneously cleaned and calibrated.

Abstract: According to one aspect, the invention relates to an aquatic sample analysis system adapted for in situ use. The system includes an incubation chamber having an optically clear portion and forming an opening for receiving a fluidic sample and apparatus for sealing the opening. The system also includes a sensor for sensing at least one parameter associated with the sample inside the chamber, a control module in communication with the sensor, and a power source.

Abstract: A system and apparatus for determining soil properties and managing the ground can include a platform equipped with traction anchors, wherein at least one anchor is configured to provide a point of traction on the ground and to pull the platform. The system also includes one or more sensors positioned and configured to detect ground properties near the anchor, and a computer subsystem configured to receive and process data on ground properties measured by the sensor, to process this information, and capable of transmitting information about the ground soil to a remote receiver.

Abstract: A plant (1) for the treatment of a multiphasic fluid and method for line-characterization of the fluid wherein the plant (1) comprises a circuit (4) within which the fluid is intended to circulate and being characterized in that it comprises a characterization tool (3) comprising at least an analysis settler (6) adapted to separate the phases to obtain so-called separated phases and a means (9) for combining the separated phases. The plant (1) is designed so that a fraction of the fluid circulating within the circuit (4) circulates within the tool (3) to pass through the analysis settler (6) so that the phases of the fluid are separated then discharged into the circuit (4). The combining means (9) is designed so as to combine the separated phases.

Abstract: A method for examining cultured cells for the presence of periodic structures comprises the steps of providing cultured cells, fixing the cultured cells, staining the fixed cultured cells using a first staining agent, stimulating the first staining agent causing it to emit light taking a two-dimensional image of the fixed cultured cells, cross-correlating first and second filtered images derived from the image of the fixed cultured cells to obtain a correlation image, and determining the presence or absence of periodic structures in the fixed cultured cells by determining whether there are periodic structures of maxima and minima in brightness in the correlation image.

Abstract: A PEFS (Piezoelectric Finger Sensor) acts as an “electronic finger” capable of accurately and non-destructively measuring both the Young's compression modulus and shear modulus of tissues with gentle touches to the surface. The PEFS measures both the Young's compression modulus and shear modulus variations in tissue generating a less than one-millimeter spatial resolution up to a depth of several centimeters. This offers great potential for in-vivo early detection of diseases. The PEFS may be incorporated into a portable hand-held device. The PEFS offers superior sensitivity.

Abstract: A nanopore FET sensor device and method of making. The nanopore FET sensor device includes a FET device stack of material layers including a source, channel and drain layers, and a nanoscale hole through the FET device stack to penult flow of strands of molecular material, e.g., DNA, therethrough. The perimeter of the nanoscale hole forms a FET device gate surface. The source and drain layers are provided with respective contacts for connection with measuring instruments that measure a flow of current therebetween. The molecular strands having charged portions pass from one side of a wafer substrate to the other side through the (nanopore) gate and modulate the current flow sensed at the source or drain terminals. The sensor collects real-time measurements of the current flow modulations for use in identifying the type of molecule. Multiple measurements by the same nanopore FET sensor are collected and compared for enhanced detection.

Abstract: In a single-use handheld diagnostic test device and an associated system and method, a biological and/or environmental test sample is received and reacted with reagents. The test device tests a single sample and includes a sensor to detect test data. The test device mates with, and transmits the test data to, an electronic device. A processor of the electronic device applies algorithms to the test data to generate highly sensitive and accurate quantitative test results. A presentation element of the electronic device presents the test results to a user. The test device is adapted for disposal, or for sterilization and re-use, after the electronic device receives the test data. The electronic device may be, for example, a mobile communications device, a personal digital assistant, a laptop computer, a navigation device, a digital audio player, a camera, or a gaming device.

Abstract: A deaeration device includes a vent structure with a porous material capable of swelling when moistened such that the vent structure can inhibit liquid from escaping while allowing for the removal of gases from liquids. The deaeration device is integrated into a blood chamber of an extracorporeal circuit. The blood chamber may further include sensor components for actively performing measurements on the blood while the deaeration device passively removes air from the blood. In an embodiment, the blood chamber may be an optical blood chamber including an optical measurement sensor that performs measurements used in a determination of a hematocrit level in the blood.

Abstract: This disclosure is directed to an apparatus, system and method for retrieving a target material from a suspension. A system includes a displacement fluid, a collector, and a primary vessel. In another implementation, the system includes a processing vessel, a displacement fluid, a collector, and a primary vessel.

Abstract: Provided are functional cell and tissue assay systems based on substrate-integrated multifunctional microelectrode arrays implementing stem cell technology. The system covers normal and pathogenic characteristics.

Abstract: A method for measuring cell cycles includes immobilizing a capture specifically binding to a cell membrane protein to a substrate, wherein the cell membrane protein is a counterpartner binding to the capture, binding a cell having the cell membrane protein as the counterpartner to the capture, and measuring a redox potential of the cell. A method for screening a substance affecting cell cycles includes immobilizing a capture specifically binding to a cell membrane protein to a substrate; wherein the cell membrane protein is a counterpartner binding to the capture, binding a cell having the cell membrane protein as the counterpartner to the capture, treating a test substance of interest for analysis to a cell, and measuring a redox potential of the cell.

Abstract: Markers useful for the identification, characterization and, optionally, the enrichment or isolation of tumorigenic cells or cell subpopulations are disclosed.

Abstract: The present invention relates to methods and assays for identifying agents capable of modulating GPR84 activity in cells, in particular agents that inhibit GPR84-agonist stimulated chemotaxis. Inhibition of GPR84-agonist stimulated chemotaxis is useful in the prevention and/or treatment of inflammatory conditions. In particular, the present invention provides methods and assays for identifying agents for use in the prevention and/or treatment of inflammatory conditions (for example inflammatory bowel diseases (IBD), rheumatoid arthritis, vasculitis, lung diseases (e.g. chronic obstructive pulmonary disease (COPD) and lung interstitial diseases (e.g. idiopathic pulmonary fibrosis (IPF)), neuroinflammatory conditions, infectious diseases, autoimmune diseases and/or diseases involving impairment of immune cell functions.

Abstract: Genetically encoded calcium indicator (GECI) polypeptides and the nucleic acid molecules encoding such polypeptides are provided. In addition, methods of using such nucleic acids and polypeptides in methods of screening for agonists or antagonists of G-protein coupled receptor (GPCR) or ion channels and methods of monitoring neural activity also are provided.

Abstract: High-throughput methods of screening agents for activities affecting renal, cardiac, blood or lymphatic vascular development and functions in amphibians in multiwell plates are provided. Also provided are novel compounds that modulate blood and lymphatic vascular development.

Abstract: The present invention relates to the field of diagnostic or analytic methods. In particular, the inventors teach that Fc?R such as CD16, CD32 or CD64 may be used for in vitro quantification of antibodies in the form of immune complexes, i.e., complexes formed by antigen and specific antibody. A method for detection and quantification of antibodies in the form of immune complexes is also provided.

Abstract: The present disclosure provides immunoassays using one or more over-labeled fluorescent probes, which provides for rapid, accurate and quantitative detection of one or more target analytes in sample, reading fluorescent intensity. The disclosed immunoassays provide multiplexing capability with low cross-reactivity.

Abstract: One aspect relates to a method of calibrating event data. The method includes obtaining, via an electronic device including a processor, event data for an assay including a reagent. The reagent is associated with one of a plurality of manufacturing lots of the reagent. The method includes receiving one or more calibration factors for the reagent based on an identifier associated with the one of the plurality of manufacturing lots. The method further includes generating calibrated event data based on an application of the one or more calibration factors to the event data.

Abstract: A method is disclosed herein for treating a polymeric surface to resist non-specific binding of biomolecules and attachment of cells. The method includes the steps of: imparting a charge to the polymeric surface to produce a charged surface; exposing the charged surface to a nitrogen-rich polymer to form a polymerized surface; exposing the polymerized surface to an oxidized polysaccharide to form an aldehyde surface; and exposing the aldehyde surface to a reducing agent. Advantageously, a method is provided which produces surfaces that resist non-specific protein binding and cell attachment and that avoids the use of photochemical reactions or prior art specially designed compounds.

Abstract: Methods of determining whether a non-diagnostic analyte is present in a non-diagnostic sample are provided. Aspects of the methods include applying a non-diagnostic sample to a sample receiving region of a lateral flow assay device and reading a detection region to determine whether a non-diagnostic analyte is present in the non-diagnostic sample. Also provided are kits that find use in practicing methods of the invention.

Abstract: Anaplasma Marginale surface protein OmpA and homologous genes from Anaplasmatacaea family members are used in compositions suitable for vaccines to treat or prevent infections caused by tick-born bacteria of the Anaplasmatacaea family. OmpA proteins or peptide fragments may be used in combination with other Anaplasmatacaea surface proteins to elicit an immune response. Furthermore, antibodies to OmpA proteins can be used in diagnostic methods to determine whether an individual has contracted an Anaplasmatacaea infection.

Abstract: A method of determining the presence or absence in a sample of a biomarker, the method comprising: (a) linking an antigen to colloidal gold to provide a gold-antigen species; (b) contacting the gold-antigen species with the sample; (c) adding a diagnosis agent to the sample; and (d) observing the color of the sample.

Abstract: The present disclosure highlights the relationship between extracellular mitochondrial components, optionally in combination with the secreted phospholipase A2-IIA and/or an auto-antibody, and in vivo as well as in vitro and inflammatory reactions/conditions, especially those released as a result of the degradation of a platelet. The present disclosure provides methods for determining the presence of inflammatory mediators, for limiting inflammatory reactions/conditions, for the diagnosis inflammatory reactions/conditions, for screening therapeutics for the treatment and/or the alleviation of symptoms of inflammatory reactions/conditions based on the detection or modulation of the level of these extracellular mitochondrial components.

Abstract: Autologous bone marrow cells (BMC) are transplanted to a heterologous site in a patient after a sample of the patient's BMC has been tested and found to have a phenotypic profile which meets minimum criteria for transplantation. The phenotypic profile may be obtained by screening a sample of bone marrow cells (BMC) from the patient for the phenotypic profile, such as a CD profile, the phenotype profile may be assessed to determine the likelihood that the BMC will be suitable for transplantation to the heterologous tissue site without enriching particular phenotypic population(s) of the BMC.

Abstract: A method for using a single sample suspected of containing a microorganism for both a local rapid test immunoassay and a remote laboratory test. The sample is collected from a patient at a physician's office or from the environment to be tested. The sample is collected using a swab or other implement, combined with a rapid test processing reagent and a portion of the processed sample is used for the local rapid test. The rapid test processing reagent typically consists of a buffer, a salt, and a detergent and is compatible with the local rapid test immunoassay. Only a portion of the processed sample is used for the local rapid test, leaving a remaining portion of the processed sample to be used in a remote laboratory assay. At least some of the remaining portion of the processed sample is combined with a stabilization agent that preserves at least the nucleic acid in the processed sample for the remote laboratory assay.

Abstract: A new coronavirus is disclosed herein with a tropism that includes humans. Means and methods are provided for diagnosing subjects (previously) infected with the virus. Also provided are among others vaccines, medicaments, nucleic acids and specific binding members.

Abstract: A method for determining, in vitro, the probability of a patient developing severe dengue, based on a blood sample, in which: a) the quantity in the blood sample of at least one marker, which is platelet factor 4, is determined; b) the quantity of platelet factor 4 determined in step a) is compared with a reference quantity of the marker obtained from a group of individuals who have been diagnosed with non-severe dengue, wherein, if the quantity of platelet factor 4 determined in step a) is less than the reference quantity established in step b), it is determined that the patient will develop severe dengue.