Patents Issued in April 19, 2018
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Publication number: 20180105821Abstract: This invention discloses a constitutive promoter from the Taro bacilliform virus (TaBV) for expression of foreign or endogenous coding sequences in plants, including dicotyledonous and monocotyledonous plants. The invention also discloses a chimeric nucleic acid construct comprising the promoter of the invention operably linked to a foreign or endogenous polynucleotide that codes for a protein of interest or a transcript capable of modulating expression of a target gene. The invention further discloses transformed plant cells, as well as differentiated plants and plant parts, containing the construct. Methods for diagnosis and treatment of viral infections, especially badnaviral infections, are also disclosed.Type: ApplicationFiled: June 28, 2016Publication date: April 19, 2018Applicant: Farmacule Bioindustries Pty LtdInventors: James Langham DALE, Robert Maxwell Harding, Douglas Kenneth Becker, Gregory John Hafner, llin Yang
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Publication number: 20180105822Abstract: The present invention provides novel DNA molecules and constructs, including their nucleotide sequences, useful for modulating gene expression in plants and plant cells. The invention also provides transgenic plants, plant cells, plant parts, seeds, and commodity products comprising the DNA molecules operably linked to heterologous transcribable polynucleotides, along with methods of their use.Type: ApplicationFiled: October 30, 2017Publication date: April 19, 2018Inventor: Stanislaw Flasinski
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Publication number: 20180105823Abstract: The present invention provides novel DNA molecules and constructs, including their nucleotide sequences, useful for modulating gene expression in plants and plant cells. The invention also provides transgenic plants, plant cells, plant parts, seeds, and commodity products comprising the DNA molecules operably linked to heterologous transcribable polynucleotides, along with methods of their use.Type: ApplicationFiled: October 30, 2017Publication date: April 19, 2018Inventor: Stanislaw Flasinski
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Publication number: 20180105824Abstract: Nucleotide sequences encoding DREB2 polypeptides are provided herein, along with plants and cells having increased levels of DREB2 gene expression, increased levels of DREB2 transcription factor activity, or both. Plants with increased levels of at least one DREB2 gene expression that exhibit increased yield, increased abiotic stress tolerance, or any combination of these, are provided. Methods for increasing yield, and abiotic stress tolerance in plants, by modulating DREB2 gene expression or activity, are also provided.Type: ApplicationFiled: March 22, 2016Publication date: April 19, 2018Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: CARL SIMMONS, SOBHANA SIVASANKAR
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Publication number: 20180105825Abstract: The present disclosure provides synthetic repressor constructs and the proteins encoded therein, as well as synthetic repressible promoter constructs for use in combination with the synthetic repressor constructs/synthetic repressors disclosed herein. Various combinations of synthetic repressor constructs and synthetic repressible promoter constructs are also provided in synthetic genetic circuits for modifying expression of a protein of interest in a plant cell.Type: ApplicationFiled: October 18, 2017Publication date: April 19, 2018Applicant: Colorado State University Research FoundationInventors: June I. Medford, Mauricio S. Antunes, Tessema K. Kassaw
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Publication number: 20180105826Abstract: A method of modifying the amount of at least one biochemical component in a plant comprising expressing Qua-Quine Starch (QQS) in the plant, the wild-type of which does not express QQS; a transgenic plant, or part thereof, which comprises and expresses QQS as a transgene and in which the amount of at least one biochemical component is modified; a tissue culture of regenerable cells of the transgenic plant; a vector comprising a nucleotide sequence, which encodes the coding sequence of QQS, operably linked to a non-native promoter, which promotes expression of the nucleotide sequence in a plant, which is other than Arabidopsis; and a method of producing a food or industrial product from a plant.Type: ApplicationFiled: November 21, 2017Publication date: April 19, 2018Inventors: Ling Li, Eve Syrkin Wurtele
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Publication number: 20180105827Abstract: A method for producing a recombinant protein in a plant, in particular a tobacco plant, preferably Nicotiana benthamiana, includes the following steps: a) culturing the plant aeroponically or hydroponically, preferably on mobile floats and under LED lighting; b) vacuum agroinfiltration of the plant obtained in a) by agrobacteria that include a DNA fragment coding for the recombinant protein; c) returning the plants to culture after step b), under the same conditions as for step a); and d) extracting and purifying the recombinant protein from the aerial portions of the plants produced in step c).Type: ApplicationFiled: November 21, 2017Publication date: April 19, 2018Inventors: Veronique GOMORD, Anne Catherine FITCHETTE, Loic FAYE
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Publication number: 20180105828Abstract: Methods and materials for modulating biomass composition in plants are disclosed. For example, nucleic acids encoding biomass composition-modulating polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having altered biomass composition and plant products produced from plants having altered biomass composition.Type: ApplicationFiled: September 27, 2017Publication date: April 19, 2018Inventor: Nestor Apuya
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Publication number: 20180105829Abstract: Methods and materials for modulating low-nitrogen tolerance levels in plants are disclosed. For example, nucleic acids encoding low nitrogen tolerance-modulating polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having increased[RCL2] low-nitrogen tolerance levels and plant products produced from plants having increased low-nitrogen tolerance levels.Type: ApplicationFiled: August 29, 2017Publication date: April 19, 2018Inventors: Gregory NADZAN, Richard SCHNEEBERGER, Han Suk KIM, David Van-dinh DANG, Kenneth A. FELDMANN, Roger PENNELL, Shing KWOK, Hongyu ZHANG, Cory CHRISTENSEN, Jack OKAMURO, Fasong ZHOU, Wuyi WANG, Emilio MARGOLLES-CLARK, Gerard MAGPANTAY, Julissa SOSA, Nestor APUYA, Kerstin PICCOLO, Bonnie HUND, Nickolai ALEXANDROV, Vyacheslav BROVER, Peter MASCIA
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Publication number: 20180105830Abstract: Provided are isolated polypeptides which are at least 80% homologous to SEQ ID NOs: 529, 475-528, 530-770, 6179-9796, 9798-10421, isolated polynucleotides which are at least 80% identical to SEQ ID NOs: 314, 1-313, 315-474, 771-6178, nucleic acid constructs comprising same, transgenic cells expressing same, transgenic plants expressing same and method of using same for increasing abiotic stress tolerance, yield, growth rate, biomass, vigor, oil content, photosynthetic capacity, seed yield, fiber yield, fiber quality, fiber length, and/or nitrogen use efficiency of a plant.Type: ApplicationFiled: December 20, 2017Publication date: April 19, 2018Applicant: Evogene Ltd.Inventors: Inbal Nurith Dangoor, Hagai Karchi
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Publication number: 20180105831Abstract: The invention provides seed and plants of spinach hybrid SVVB5191 and the parent lines thereof. The invention thus relates to the plants, seeds and tissue cultures of spinach hybrid SVVB5191 and the parent lines thereof, and to methods for producing a spinach plant produced by crossing such plants with themselves or with another spinach plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the leaf and gametes of such plants.Type: ApplicationFiled: August 2, 2017Publication date: April 19, 2018Inventor: John Meeuwsen
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Publication number: 20180105832Abstract: The technology provided herein relates to novel methods and compounds for a multi-species pathogen infection control. In particular, the present disclosure pertains to methods of inhibiting the growth of a target pathogen expressing the cysteine-rich secreted protein (CSP), whereby the method comprises contacting said target pathogen with an inhibitor against said CSP, wherein said inhibitor inhibits the CSP expression and/or binds to a protein product of a gene coding CSP. Nucleic acid molecules encoding said inhibitors, vectors and host cells containing the nucleic acids and methods for preparation and producing such inhibitors are also disclosed, as well as the use of said CSP-inhibitors for the control/treatment of diseases associated with a microbial pathogen expressing CSP.Type: ApplicationFiled: April 13, 2015Publication date: April 19, 2018Inventors: Greta Noelke, Stefan Schillberg, Max Schubert
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Publication number: 20180105833Abstract: An inducible expression cassette for controlled expression of multiple genes includes the DNA sequences of a bidirectional promoter inserted between two DNA sequences. Transfection into a host cell and the two DNA sequences encode genes of interest, provides one DNA sequence expressed constitutively and manipulation of the expression of the other DNA sequence. A regulatory DNA cassette containing another bidirectional promoter and two DNA sequences that encode a marker and a regulatory expression product is also disclosed. Both cassettes can be incorporated in a non-viral vector, like the Sleeping Beauty transposon, or a viral vector to induce controlled expression of multiple genes into host cells. A kit containing a package of each above vector type is also disclosed, as is a method of transforming a host cell.Type: ApplicationFiled: March 16, 2016Publication date: April 19, 2018Inventors: Andrew WILBER, William Halford
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Publication number: 20180105834Abstract: The present invention provides a method of site-directed insertion to H11 locus in pigs by using site-directed cutting system, includes the following steps: 1) identify the targeted sequence targeted by the targeted cutting system in the targeted genome sequence of pigs; 2) design and construct the targeting sequence of the corresponding cutting system according to the targeted site; 3) construction of targeting vector; 4) transfect cells, identify the efficiency of fixed-point insertion by PCR amplification. The invention is dependent on the site-directed cutting system of H11 locus in pigs, to insert the target gene into the target site, in order to solve the problems such as low efficiency of traditional shooting technique, inconvenience design of PCR detection primer, harder to detect. The invention provides a method of site-directed insertion which can stably express the foreign gene at the H11 locus, to build an efficient platform for the production of transgenic pigs.Type: ApplicationFiled: November 27, 2014Publication date: April 19, 2018Inventors: Kui Li, Jinxue Ruan, Shulin Yang, Yulian Mu, Hegang Li, Tianwen Wu, Jingliang Wei, Kui Xu, Lei Huang, Rong Zhou, Nan Liu
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Publication number: 20180105835Abstract: Methods and compositions using a CRISPR-Cas Type IIIA resulting in RNA gene knockdown and knockout in an animal.Type: ApplicationFiled: August 31, 2017Publication date: April 19, 2018Inventors: Thomas FRICKE, Matthias BOCHTLER, Gintautas TAMULAITIS, Migle KAZLAUSKIENE, Virginijus SIKSNYS
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Publication number: 20180105836Abstract: The present document describes a process for production of hydrogen gas (H2) from fermentation of crude glycerol with a hydrogen producing microorganism in a bioreactor. The process comprises the step of introducing a volume of crude glycerol in a fermentation mixture which comprises a fermentation medium comprised of crude glycerol and hydrogen producing microorganisms under a fermentative hydrogen production condition, and then removing a volume of the fermentation mixture equal to the second volume of the crude glycerol, to maintain constant the total volume of the fermentation mixture.Type: ApplicationFiled: October 14, 2016Publication date: April 19, 2018Inventors: Saurabh Jyoti SARMA, Sampa MAITI, Satinder Kaur BRAR, Yann LEBIHAN, Gerardo BUELNA, Mausam VERMA, Vinayak PACHAPUR, Ratul Kumar DAS
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Publication number: 20180105837Abstract: The present invention provides a method of producing ?-santalene, said method comprising contacting at least one polypeptide with farnesyl pyrophosphate (FPP). In particular, said method may be carried out in vitro or in vivo to produce ?-santalene, a very useful compound in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of a polypeptide useful in the method of the invention. A nucleic acid encoding the polypeptide of the invention and an expression vector containing said nucleic acid are also part of the present invention. A non-human host organism or a cell transformed to be used in the method of producing ?-santalene is also an object of the present invention.Type: ApplicationFiled: August 29, 2017Publication date: April 19, 2018Applicant: Firmenich SAInventor: Michel SCHALK
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Publication number: 20180105838Abstract: The invention relates to microbial terpene production. Known methods for microbial production of terpenes are mostly based on the direct conversion of sugars. Therefore alternative substrates, in particular alternative carbon sources, for use in microbial terpene production were desirable. The invention relates to a methylotrophic bacterium containing recombinant DNA coding for at least one polypeptide with enzymatic activity for heterologous expression in said bacterium, wherein said at least one polypeptide with enzymatic activity is selected from the group consisting an enzyme of a heterologous mevalonate pathway, a heterologous terpene synthase and optionally a heterologous synthase of a prenyl diphosphate precursor. The invention further relates in particular to a method for de novo microbial synthesis of sesquiterpenes or diterpenes from methanol and/or ethanol.Type: ApplicationFiled: March 11, 2016Publication date: April 19, 2018Inventors: Jens SCHRADER, Markus BUCHHAUPT, Frank SONNTAG, Cora KRONER, Heike BRÜSER, Hartwig SCHRÖDER, Ralf PELZER
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Publication number: 20180105839Abstract: The present invention relates to compositions and methods of producing xanthophylls in microorganisms.Type: ApplicationFiled: September 1, 2017Publication date: April 19, 2018Inventor: Yechun Wang
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Publication number: 20180105840Abstract: The present invention provides a process for industrial production of chiral-1,1-difluoro-2-propanol. More specifically, a microorganism having the activity to cause asymmetric reduction of 1,1-difluoroacetone or an enzyme having the same activity is allowed to act on 1,1-difluoroacetone, whereby chiral-1,1-difluoro-2-propanol can be produced with high optical purity and in good yield. The process for production of the present invention is easy for industrial implementation.Type: ApplicationFiled: March 2, 2016Publication date: April 19, 2018Applicants: PUBLIC UNIVERSITY CORPORATION TOYAMA PREFECTURAL UNIVERSITY, CENTRAL GLASS COMPANY, LIMITEDInventors: Yasuhisa ASANO, Kimiyasu ISOBE, Ryoko UEDA, Tetsuro NISHII, Akihiro ISHII
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Publication number: 20180105841Abstract: The present invention relates to processes for producing ethanol from starch-containing material using a Saccharomyces cerevisiae yeast deposited under Accession No. V15/004035, V15/004036 or V15/004037 at National Measurement Institute, Victoria, Australia) or a fermenting organism strain having properties that are about the same as that of the deposited Saccharomyces cerevisiae strain or a derivative of Saccharomyces strain V15/004035, V15/004036 or V15/004037 having the defining characteristics of strain V15/004035, V15/004036 or V15/004037. The invention also relates to Saccharomyces yeast deposited under the Budapest Treaty and having NMI accession no. V15/004035, V15/004036 or V15/004037 or a derivative of strain V15/004035, V15/004036 or V15/004037 which exhibits one or more properties or defining characteristics of strain V15/004035, V15/004036 or V15/004037.Type: ApplicationFiled: March 17, 2016Publication date: April 19, 2018Applicants: Novozymes A/S, Microbiogen Pty. Ltd.Inventors: Jennifer Headman, Nathaniel Edward Kreel, Ryan Schron, Paul Victor Attfield, Philip John Livingstone Bell, Alan Jay House, Christie Strahler, Harry Showmaker
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Publication number: 20180105842Abstract: Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can convert feedstock materials to a sugar solution, which can then be fermented to produce ethanol. Biomass feedstock is saccharified in a vessel by operation of a jet mixer, the vessel also containing a liquid medium and a saccharifying agent.Type: ApplicationFiled: December 14, 2017Publication date: April 19, 2018Inventors: Marshall Medoff, Thomas Craig Masterman
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Publication number: 20180105843Abstract: The invention relates to an alcoholic fermentation process in the presence of one or more high alcohol tolerant yeast and one or more maltotriose positive yeast. The process of the present invention can be a fermentation process for the production of ethanol, for the production of beer, for the production of wine and the like. In a preferred embodiment, the present invention relates to a process for the production of ethanol in the presence of distiller's yeast and baker's yeast.Type: ApplicationFiled: December 15, 2017Publication date: April 19, 2018Inventors: Jean-Claude Marie Pierre DE TROOSTEMBERGH, Bernhard Horbach, Michael Josef Christian Kruse, Nicolas Andre Albert Meurens
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Publication number: 20180105844Abstract: Provided is a method of preparing cinnamaldehyde by using a recombinant microorganism.Type: ApplicationFiled: May 13, 2016Publication date: April 19, 2018Inventors: Ki Jun JEONG, Sun Chang KIM, Hyun Bae BANG, Yoon Hyeok LEE, Suk Chae JUNG
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Publication number: 20180105845Abstract: The present invention relates to a recombinant microorganism capable of producing 2,4-dihydroxybutyrate, which is characterized by an increased cellular export, and preferably by a decreased cellular import, of said 2,4 DHB. The invention also relates to a method for the optimized productionof 2,4-dihydroxybutyrate by culturing said microorganism in a fermentation medium and recovering 2,4-DHB from said medium.Type: ApplicationFiled: April 7, 2016Publication date: April 19, 2018Applicant: METABOLIC EXPLORERInventors: Wanda DISCHERT, Laurence DUMON-SEIGNOVERT, Perrine VASSEUR, Gwénaëlle BESTEL-CORRE, Philippe SOUCAILLE
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Publication number: 20180105846Abstract: The present disclosure provides a method for extracting alpha-ketoglutarate and pyruvate simultaneously from microbial fermentation broth or enzyme transformation solution, which is related to the technical field of biological separation and extraction.Type: ApplicationFiled: July 19, 2017Publication date: April 19, 2018Inventors: Jingwen Zhou, Jian Chen, Weizhu Zeng, Guocheng DU, Fang Fang, Song Liu
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Publication number: 20180105847Abstract: The invention relates to a genetically engineered bacterium having an enzyme that converts acetyl-CoA to acetoacetyl-CoA, an enzyme that converts acetoacetyl-CoA to 3-hydroxybutyryl-CoA, and an enzyme that converts 3-hydroxybutyryl-CoA to 3-hydroxybutyrate. The bacterium may also have enzymes to produce other downstream products, such as 3-hydroxybutyryaldehyde, and 1,3-butanediol. Typically, the bacterium is capable of producing these products from a gaseous substrate, such as syngas or an industrial waste gas.Type: ApplicationFiled: July 25, 2017Publication date: April 19, 2018Inventors: Michael Koepke, Rasmus Overgaard Jensen, James Bruce Yarnton Haycock Behrendorff, Ryan Edward Hill, Darmawi Juminaga, Alexander Paul Mueller
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Publication number: 20180105848Abstract: Nucleic acids and cells comprising a methyltransferase gene and/or a reductase gene are disclosed. These nucleic acids and cells may be used to produce branched (methyl)lipids, such as 10-methylstearate.Type: ApplicationFiled: September 20, 2017Publication date: April 19, 2018Inventors: Arthur J. Shaw, IV, Hannah Blitzblau, Donald V. Crabtree
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Publication number: 20180105849Abstract: A method for producing lysophosphatidylethanolamine 18:1 includes treating phospholipids extracted from a microorganisms of Pseudomonas sp. with phospholipase A2. The lysophosphatidylethanolamine 18:1 can be used as a plant vaccine material for preventing the plants from injuries caused by pathogen infections and/or environmental stresses and accelerating the recovery of plants injured by pathogen infections and/or environmental stresses, and can also be used as a composition for enhancing fruit ripening (color and sweetness) and storage properties, and as it can be used for an application in plant tissues, food products, pharmaceuticals, cosmetics, and agricultural use, it would be very advantageously used in related industries.Type: ApplicationFiled: December 22, 2017Publication date: April 19, 2018Inventors: Beung Tae Ryu, Young Pyo Lee, Ji Hye Jung, Sung Hee Jung
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Publication number: 20180105850Abstract: The present invention relates to the provision of genetically modified microbial cells, such as yeast cells with an improved ability for producing L-ornithine and its derivatives. Overproduction of L-ornithine is obtained in the first place by the down-regulation or attenuation of specially selected genes, wherein said genes encode enzymes involved in the L-ornithine consumption and/or degradation pathways. Further L-ornithine production ability is improved by down-regulation, attenuation, deletion or overexpression of specially selected genes, wherein said genes encode enzymes and/or proteins involved in the L-ornithine ‘acetylated derivatives cycle’, L-glutamate synthesis pathways, subcellular trafficking, TCA cycle, pyruvate carboxylation pathway, respiratory electron-transport chain, and the carbon substrates' assimilation machinery. The invention additionally provides a method to produce L-ornithine with said modified eukaryotic cells.Type: ApplicationFiled: March 10, 2016Publication date: April 19, 2018Inventors: Jiufu Qin, Anastasia Krivoruchko, Florian David, Bo Jiang, Jens Nielsen
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Publication number: 20180105851Abstract: The present invention provides a method and a system for preparing pulp for paper with grass straws as raw material. The method of the present invention includes the following steps: 1) adding water in raw material for pulping, performing a first enzymolysis with ligninolytic enzyme; 2) cooking the first enzymolysis product at 80-130° C.; 3) performing second enzymolysis with hemicellulase to the cooked product. The method of the present invention is able to improve the pulp yield, reduce discharge of pollutants from black liquor, lower alkali consumption and energy consumption, and facilitate extraction of lignin and C-5 sugars, and pulp has a high quality.Type: ApplicationFiled: May 24, 2017Publication date: April 19, 2018Inventors: HUA SUN, ZHONGYU LIU
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Publication number: 20180105852Abstract: Provided is a method of preparing nanoparticle-type polynucleotides, the method comprising forming the polynucleotides comprising modified nucleotides, in which the forming includes chemically synthesizing the polynucleotides comprising modified nucleotides, synthesizing the polynucleotides comprising modified nucleotides using an enzyme, or a combination thereof.Type: ApplicationFiled: February 28, 2017Publication date: April 19, 2018Inventors: Dae Ro AHN, Kyoung Ran KIM
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Publication number: 20180105853Abstract: Provided herein, among other things, is a method for producing a ligation product on a support. In some embodiments, the method may comprise hybridizing a first double-stranded oligonucleotides and a second double-stranded oligonucleotide to a substrate comprising surface-tethered oligonucleotides and ligating the distal ends of the first and second double-stranded oligonucleotides together, thereby producing a first ligation product that is tethered to the support at both ends.Type: ApplicationFiled: October 12, 2017Publication date: April 19, 2018Inventors: Nicholas M Sampas, Jeffrey R Sampson
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Publication number: 20180105854Abstract: Non-naturally occurring tRNASec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNASec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNASec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNASec.Type: ApplicationFiled: October 4, 2017Publication date: April 19, 2018Inventors: Dieter Söll, Caroline Aldag, Michael Hohn, Takahito Mukai
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Publication number: 20180105855Abstract: Embodiments disclose apparatus, methods and software for performing biological screening and analysis implemented using an instrument platform capable of detecting a wide variety of cell-based secretions, expressed proteins, and other cellular components. The platform may be configured for simultaneous multiplexed detection of a plurality biological components such that a large number of discrete samples may be individually sequestered and evaluated to detect or identify constituents from the samples in a highly parallelized and scalable manner.Type: ApplicationFiled: December 3, 2015Publication date: April 19, 2018Inventors: Patrick PACZKOWSKI, Sean MACKAY, Sean MCCUSKER
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Publication number: 20180105856Abstract: A composition is provided. The composition comprises an enzyme and a ruthenium- or osmium-based electron transfer agent, wherein the ruthenium- or osmium-based electron transfer agent is a complex [M(A)x(B)y] (X)n wherein M is ruthenium or osmium; A is an amine ligand; each B is a ligand different to A; x is an integer selected from 1 to 5; y is an integer selected from 1 to 5; x+y is 6 or 8; n is an integer selected from 1 to 6; and X is any suitable counterion.Type: ApplicationFiled: April 28, 2016Publication date: April 19, 2018Applicant: Inside Biometrics LimitedInventors: Marco Cardosi, Stephanie Kirkwood
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Publication number: 20180105857Abstract: A biosensor for the detection of an analyte such as ascorbic acid is provided. Also provided is a device comprising the biosensor. In addition, methods of detecting analytes such as ascorbic acid in a sample, and methods of a point-of-care diagnosis of eye disease and eye injury are provided.Type: ApplicationFiled: April 1, 2016Publication date: April 19, 2018Inventors: Dipanjan Pan, Santosh Kumar Misra, Manas Ranjan Gartia, Leanne T. Labriola
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Publication number: 20180105858Abstract: A computer of a prediction-rule generating system includes an input unit configured to input time series data of an abundance proportion of microorganisms or nucleotide sequences included in activated sludge in which a water treatment is performed and water quality information indicating water quality after a water treatment associated with data at each time constituting the time series data, a principal component analyzing unit configured to perform principal component analysis on the input time series data and calculate principal component scores of data at each time constituting time series data, and a prediction rule generating unit configured to generate a prediction rule for predicting water quality after a water treatment from an abundance proportion of microorganisms or nucleotide sequences on the basis of the calculated principal component scores and the input water quality information indicating water quality after a water treatment.Type: ApplicationFiled: March 30, 2016Publication date: April 19, 2018Applicant: Sumitomo Chemical Company, LimitedInventors: Akiko Nakazono, Fumiyoshi Okazaki, Hiroyuki Asako
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Publication number: 20180105859Abstract: Disclosed herein are methods and devices for using digital isothermal amplification to detect subtle responses to environmental stimuli, such as detecting antibiotic susceptibility using digital quantification of DNA replication and/or chromosome segregation.Type: ApplicationFiled: September 25, 2017Publication date: April 19, 2018Inventors: Rustem F. Ismagilov, Nathan G. Schoepp, Travis S. Schlappi, Matthew S. Curtis
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Publication number: 20180105860Abstract: The invention relates to muteins of the pyrroline-5-carboxylate reductase 1 (PYCR1), to nucleic acid molecules comprising a nucleotide sequence encoding such muteins, to methods of determining in a subject a predisposition of having an age related disorder associated with PYCR1, to methods of identifying a compound capable of modifying the expression of PYCR1, and methods of treating a subject having an age-related disorder associated with PYCR1. The invention further relates to a genetically modified animal and a method of modifying the expression of the PYCR1 gene in an animal.Type: ApplicationFiled: November 21, 2017Publication date: April 19, 2018Inventors: BRUNO REVERSADE, Stefan Mundlos
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Publication number: 20180105861Abstract: The invention relates to methods, compositions and kits for determining the cleanness of a surface. Described herein is a kit for determining the cleanness of a surface comprising an enzymatic solution comprising catalase; and a developer solution comprising hydrogen peroxide. Described also is a method for determining the cleanness of a surface comprising: applying on a zone of a surface to be cleaned an enzymatic solution comprising catalase and letting it dry; after cleaning said surface, applying on said zone a developer solution comprising hydrogen peroxide; and detecting a catalytic reaction between remaining catalase and hydrogen peroxide, wherein presence of a catalytic reaction is indicative of a surface not properly cleaned. Also provided are specific enzymatic solutions and developer solutions for the kits and methods.Type: ApplicationFiled: October 18, 2016Publication date: April 19, 2018Applicant: Sani-Marc, Inc.Inventors: Dominic DESROSIERS, Patrick MARCHAND
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Publication number: 20180105862Abstract: An electrochemical method is provided for in-vitro determination of the presence of bacteria producing lactamases, in a sample that may contain such bacteria.Type: ApplicationFiled: April 1, 2016Publication date: April 19, 2018Inventors: Murielle DEQUAIRE-ROCHELET, Alain HARTMANN, Catherine NEUWIRTH, Benoit CHANTEMESSE
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Publication number: 20180105863Abstract: Methods are provided of treating obesity or an obesity comorbidity in a mammalian subject comprising administering to the subject an amount of an agent effective to treat obesity or the obesity comorbidity, which agent inhibits (i) I?B kinase (IKK?) activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-?B) or (ii) Notch signaling, in a manner so as to permit the agent to enter the hypothalamus of the subject. Assays are also provided for identifying candidate agents for treating obesity’.Type: ApplicationFiled: October 13, 2017Publication date: April 19, 2018Applicant: ALBERT EINSTEIN COLLEGE OF MEDICINE, INC.Inventor: Dongsheng Cai
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Publication number: 20180105864Abstract: The present invention provides a method of determining integrity and/or quantity of cell free DNA (cfDNA) in a bio logical sample comprising amplifying target sequences with at least a first primer/probe set and at least a second primer probe/set, amplifying the target sequences of differing lengths, and monitoring for detection of the labels of the oligonucleotide probes, and determining the integrity and/or quantity of the cfDNA based on the level of detection of the label of the oligonucleotide probe from the first primer/probe set compared to the level detection of the label of the oligonucleotide probe from the second primer/probe set. The present invention also provides methods for generating a library with the cfDNA for sequencing and analysis.Type: ApplicationFiled: April 18, 2016Publication date: April 19, 2018Inventors: Muhammed Murtaza, Tania Contente-Cuomo
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Publication number: 20180105865Abstract: Methods are provided for diagnosing whether a subject has, or is at risk of developing pancreatic cancer. The methods include measuring the level of at least one miR gene product in a biological sample derived from the subject's pancreas. An alteration in the level of the miR gene product in the biological sample as compared to the level of a corresponding miR gene product in a control sample, is indicative of the subject either having, or being at risk for developing, pancreatic cancer.Type: ApplicationFiled: September 5, 2017Publication date: April 19, 2018Inventors: Thomas D. SCHMITTGEN, Daniel J. BRACKETT
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Publication number: 20180105866Abstract: This disclosure describes example techniques and systems for identifying the presence and/or composition of nucleic acids in the blood of a host organism of a model species harboring tissue of a donor organism of another species. For example, the technique may involve identifying the presence and composition of nucleic acids in the blood of a mouse harboring tissue of a human or another companion animal. These cell-free nucleic acids that are identified can be used as biomarkers to determine the presence of a disease, its biological behavior, its rate of progression, and/or the response of the disease to one or more unique therapies. In other examples, the cell-free nucleic acids may be used as biomarkers to determine a response of the host species to the tissue of the donor organism or a response of tissue derived from the second organism to transplantation within the first organism of the first species.Type: ApplicationFiled: October 13, 2017Publication date: April 19, 2018Inventors: Jaime F. Modiano, Milcah C. Scott, John R. Garbe
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Publication number: 20180105867Abstract: Provided herein are methods of detecting a target nucleic acid sequence. In one embodiment, the method includes contacting genomic DNA with a guide RNA having a portion complementary to the target sequence in the genomic DNA and with Cas9 nickase to produce a single-strand break in the genomic DNA at a specific location adjacent to the target sequence. The method further includes contacting the nicked DNA with a polymerase and fluorescently labeled nucleotide. The fluorescently labeled nucleotide is incorporated into the nicked DNA at the specific location and the target nucleic acid sequence is detected via fluorescent label.Type: ApplicationFiled: October 18, 2017Publication date: April 19, 2018Inventors: Ming Xiao, Harold C. Riethman, Wenhui Hu, Jennifer McCaffrey
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Publication number: 20180105868Abstract: Method and media for diagnosing Crohn's disease are provided. A method of diagnosing Crohn's disease in patients includes: obtaining a sample from an individual; culturing the sample to determine the presence or absence of Mycobacterium avium subspecies hominissuis (MAH) in the sample; and diagnosing the individual with Crohn's disease based on the determining the presence of MAH in the sample.Type: ApplicationFiled: November 21, 2017Publication date: April 19, 2018Inventors: Peilin Zhang, Lawrence M. Minardi
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Publication number: 20180105869Abstract: A method for analyzing planar sample is provided. In some cases the method comprises: (a) incubating the planar sample with a capture agent that is linked to an oligonucleotide, wherein the capture agent specifically binds to complementary sites in the planar sample; (b) reading a fluorescent signal caused by extension of a primer that is hybridized to the oligonucleotide, using fluorescence microscopy. Several implementations of the method, and multiplexed versions of the same, are also provided.Type: ApplicationFiled: December 5, 2017Publication date: April 19, 2018Inventors: Nikolay Samusik, Garry P. Nolan, Yury Goltsev
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Publication number: 20180105870Abstract: Described herein are methods, compositions and kits for identifying modifications that could lead to false positive detections in nucleic acid sequencing. In some embodiments, the methods, compositions and kits provided herein are useful for reducing potential of false positive detection of variants caused by errors during sample preparation or sequencing.Type: ApplicationFiled: October 14, 2016Publication date: April 19, 2018Inventors: Douglas A. Amorese, Stephanie C. Huelga, Bin Li