Abstract: The present invention provides methods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the invention provides a method comprising culturing cells expressing a) a small interfering RNA (siRNA) specific for a lactate dehydrogenase (LDH) and b) an siRNA specific for a pyruvate dehydrogenase kinase (PDHK). In another aspect, the invention provides cultured cells or vectors comprising an siRNA specific for a LDH and an siRNA specific for a PDHK.

Abstract: The invention provides mannosidases capable of uncapping mannose-1-phospho-6-mannose moieties and demannosylating phosphorylated N-glycans, methods of using such mannosidases, glycoproteins produced using the methods, as well as methods of facilitating mammalian cellular uptake of glycoproteins.

Abstract: In the course of the present invention, it was discovered that one could regulate association between polypeptides by modifying amino acid residues that form the interface during the association to amino acids carrying the same type of charge. In this context, the present invention enables efficient formation of heterologous molecules. For example, the present invention can be suitably applied to the preparation of bispecific antibodies.

Abstract: The invention relates to methods for producing mogrosides with the aid of enzymes. In particular the invention proposes various biosynthetic pathways useful for mogroside production and enzymes useful for mogroside production are provided. Furthermore, the invention provides recombinant hosts useful in performing the methods of the invention.

Abstract: The invention relates to a mass-spectrometric method to determine microbial resistances to antibiotics, in which the microbes are cultured in a medium comprising an antibiotic, and a mass spectrum of the microbes is acquired after they have been cultured. The method is characterized by the fact that any microbial growth taking place during the culture is mass-spectrometrically determined with the aid of a reference substance, which is added in a dosed amount and is co-measured in the mass spectrum, wherein a growth in microbes indicates the resistance to the antibiotic.

Abstract: Nucleic acid constructs and methods that provide superior prevention of primer-dimers and other artifacts of false priming events are disclosed. In particular, there is disclosed a hairpin primer having a target-specific primer region, wherein the target-specific region comprises a target-binding dependent cleavage sequence; a first stem forming region 5? of the target-specific primer region; and a second stem forming region 3? of the target-specific primer region, wherein the second stem forming region is complementary to the first stem forming region. Methods of using the hairpin primer to amplify a target nucleic acid are also disclosed.

Abstract: The present invention is directed to methods for identifying the presence of one or more methylated or unmethylated target nucleotide sequences in a sample that involve a nuclease-ligation reaction. In some embodiments, the ligation products formed in the nuclease-ligation process of the present invention are subsequently amplified using a polymerase chain reaction. The ligated product sequences or extension products thereof are detected, and the presence of one or more methylated or unmethylated target nucleotide sequences in the sample is identified based on the detection.

Abstract: A method of determining the risk of cognitive decline in an aging subject is provided. The method includes analyzing an MRNA transcript including a GRIN2B nucleic acid sequence for the presence of the A allele in a biological sample obtained from the subject. The method also includes identifying the subject as having a decreased risk of cognitive decline when the A allele is present.

Abstract: A method of morphological reconstruction of biological activity in a tissue sample maps biological data resulting from analysis of tissue samples onto a 3-D morphological rendering of the biological sample. Each slice in a set of histological slices, indexed by a first index, is micro dissected into micro samples indexed by a pair of first and second indices. The indices are utilized to spatially map biological data to the 3-D rendering.

Abstract: The invention generally relates to assemblies for displacing droplets from a vessel that facilitate the collection and transfer of the droplets while minimizing sample loss. In certain aspects, the assembly includes at least one droplet formation module, in which the module is configured to form droplets surrounded by an immiscible fluid. The assembly also includes at least one chamber including an outlet, in which the chamber is configured to receive droplets and an immiscible fluid, and in which the outlet is configured to receive substantially only droplets. The assembly further includes a channel, configured such that the droplet formation module and the chamber are in fluid communication with each other via the channel. In other aspects, the assembly includes a plurality of hollow members, in which the hollow members are channels and in which the members are configured to interact with a vessel.

Abstract: The present disclosure relates to systems and methods for nucleic acid ligation. In particular, the present disclosure provides oligonucleotide adaptors for use in nucleic acid ligation reactions.

Abstract: This invention is directed to a guide positioning sequencing technology of whole-genome DNA methylation. The invention provides a new detection method of nucleic acid methylation. In particular, a concept of “positioning” in the detection of nucleic acid methylation is provided. Specifically, a portion of a sequence is used for genome wide positioning and the other portion of the sequence is used for methylation detection in sequencing, thereby solving/defeating previously existing challenges in methylation detection and bioinformatics analysis of a genome.

Abstract: Methods, reagents, compositions, and kits for reactivity-dependent polymerase chain reaction (RD-PCR) and interaction-dependent polymerase chain reaction (ID-PCR) are provided herein. RD-PCR is a technique useful for determining whether a reactive moiety can form a covalent bond to a target reactive moiety, for example, in screening a library of candidate reactive moieties for reactivity with a target reactive moiety, and in identifying an enzyme substrate, for example, in protease substrate profiling. ID-PCR is a technique useful for determining whether a ligand can non-covalently bind to a target molecule, for example, in screening a library of candidate ligands for non-covalent interaction with a target molecule. RD-PCR and ID-PCR are also useful in detecting the presence of an analyte or an environmental condition.

Abstract: The present invention relates to a scalable multiplex PCR method that can simultaneously amplify overlapping amplicons without the drawbacks of conventional multiplex PCR. The method selectively amplifying target nucleic acid fragments having an overlapping region. The method comprises the steps of: obtaining a first nucleic acid sequence comprising a first tag t2 and a first forward primer F1, obtaining a second nucleic acid sequence comprising a second tag t1 and a first reverse primer R1, obtaining a third nucleic acid sequence comprising the second tag t1 and a second forward primer F2, obtaining a fourth nucleic acid sequence comprising a third tag t3 and a second reverse primer R2, wherein each primer is a gene-specific primer; performing initial cycles of PCR; and then performing later cycles of PCR at higher annealing temperatures to obtain amplification products.

Abstract: The present disclosure provides methods and compositions for sequencing nucleic acid molecules and identifying individual sample nucleic acid molecules using Molecular Index Tags (MITs). Furthermore, reaction mixtures, kits, and adapter libraries are provided.

Abstract: The present disclosure provides compositions and methods for accurately detecting mutations by uniquely tagging double stranded nucleic acid molecules with dual cyphers such that sequence data obtained from a sense strand can be linked to sequence data obtained from an anti-sense strand when sequenced, for example, by massively parallel sequencing methods.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

Abstract: The invention relates to methods for diagnosing Parkinson's Disease PAD) with miRNA markers. Towards the identification of biomarkers for diagnosis of PD, a comprehensive analysis of miRNA expression patterns was obtained. Significantly deregulated miRNAs were identified.

Abstract: Compositions and methods are provided for diagnosis, prognosis and treatment of AR-related diseases, such as prostate diseases, such as prostate cancer, breast cancer, and many other diseases. In particular, a novel and clinically relevant mutation at position 877 of the androgen receptor (AR) has been identified. Drug sensitivity can be predicted and therapeutic regimens can be planned on the basis of the presence or absence of this mutation. Polypeptides comprising, antibodies to, and polynucleotides encoding the mutant AR can be used to identity novel treatments. A double mutation in AR at positions 742 and 878 is also shown to be useful for patient stratification.

Abstract: Described herein are methods for the diagnosis, prognosis, and treatment of neurological conditions, e.g. Huntington's Disease, relating to the misregulation of miRNAs in such conditions.

Abstract: This disclosure generally relates to a method, a system and a kit for diagnosing and treating various disease conditions using the gene expression value in a given sample obtained from a subject. This disclosure further relates to selecting the right set of genes as a portfolio of genes for a particular disease. Providing a set of appropriate portfolio of gene for studying the gene expression values and using the values as a diagnostic tool is also disclosed. The gene expression value is further used for intervention by pharmaceuticals as a prediction model for the treatment of the disease. An individual may have a personalized health card that may store the gene expression value for that particular individual. A system may store the data for portability and integrated view of the individual's medical data for effective diagnosis and treatment.

Abstract: The disclosure demonstrates the correlation of the methylation status of the miR-193a gene and the expression level of the miR-193a-3p transcript with the resistance of bladder cancer cells to certain chemotherapeutic drugs including pirarubicin (Pi), pacilitaxol (Pa), adriamycin (Ad), epirubicin hydrochloride (EH), and cispaltin (Ci), but not others (e.g, hydroxycamptothecin (Hy), gemcitabine (Ge) and mitomycin (Mi)). Further, the disclosure identifies seven target genes (SRSF2, PLAU, HIC2, LOXL4, HOXC9, PSEN1, and ING5) that are directly regulated by miR-193a-3p. The methylation/expression status of miR-193a-3p alone or in combination of the expression level of the target genes can serve as valuable indicators for chemotherapy outcome of a bladder cancer patient with a corresponding chemotherapeutic drug, and can be drug targets for sensitizing a bladder cancer patient for that drug.

Abstract: Provided herein is technology relating to performing methylation assays. In particular, the technology relates to internal controls for methylation assays.

Abstract: The disclosure provides methods and compositions useful for identifying a subject's predisposition to a gastrointestinal disease or disorder.

Abstract: MicroRNAs are provided for evaluating the physiological and/or pathological condition of a subject; and a kit thereof is provided for evaluating the physiological and/or pathological condition of a subject, wherein the kit contains the tools for determining all detectable microRNAs that stably existing in the serum/plasma of a subject; and a biochip for evaluating the physiological and/or pathological condition of a subject, wherein the biochip contains the components for determining all detectable microRNAs stably existing in the serum/plasma of a subject. The aforementioned combination, method, kit and biochip can be used for diagnosis as well as differentially diagnosis of diseases including various tumors.

Abstract: [PROBLEM TO BE SOLVED] An object of the present invention is to provide a novel tumor marker and use thereof. In more detail, the present invention provides a novel tumor marker, a method for measuring said tumor marker and a measurement kit, a method for detecting cancer using the same, a kit for detecting cancer, a method for screening a preventive and/or therapeutic agent for cancer, as well as a medicament such as cancer vaccine. [SOLUTION] According to the present invention, a method for measuring an alternative splicing variant of OATP1B3 mRNA in a sample to be examined is provided. Said measurement method comprises measuring mRNA comprising a nucleotide sequence represented by SEQ ID NO: 1 in the sequence listing table in a sample to be examined isolated from living organism with differentiation from a mRNA comprising a nucleotide sequence represented by SEQ ID NO: 2. Said measurement method is useful for detecting cancer or screening a preventive and/or therapeutic agent.

Abstract: A photo-responsive composite actuator according to the present invention includes a polymeric scaffold film; an azobenzene liquid crystal polymer applied on a surface of the polymeric scaffold film; and a protective film attached to a surface of the azobenzene liquid crystal polymer.

Abstract: Methods, reagents and compositions thereof for predicting RA onset in susceptible individuals, diagnosing RA onset, and/or evaluating efficacy of a therapeutic regimen for treating RA are described herein. Determining the amount of a particular bacterial species comprising SEQ ID NO: 1, SEQ ID NO: 2, or SEQ ID NO: 3 (SEQ ID NO: 1+ bacteria) serves as a biomarker for the above indications.

Abstract: Methods of predicting whether a subject has a cervical intraepithelial neoplasia (CIN) lesion are provided. Aspects of the methods include obtaining both morphometric and biomarker data from a liquid cervical cellular sample and then using both types of data to predict whether the subject has a CIN lesion. Also provided are systems that find use in practicing the methods. The methods and systems find use in a variety of applications, including cervical cancer screening applications.

Abstract: A method of producing an article selected from a titanium article and a titanium alloy article comprises melting feed materials with a source of hydrogen to form a molten heat of titanium or a titanium alloy, and casting at least a portion of the molten heat to form a hydrogenated titanium or titanium alloy ingot. The hydrogenated ingot is deformed at an elevated temperature to form a worked article comprising a cross-sectional area smaller than a cross-sectional area of the hydrogenated ingot. The worked article is dehydrogenated to reduce a hydrogen content of the worked article. In certain non-limiting embodiments of the method, the dehydrogenated article comprises an average ?-phase particle size of less than 10 microns in the longest dimension.

Abstract: A grain-oriented electrical steel sheet to which electron beam irradiation is applied, has a film and a thickness of t (mm), wherein no rust is produced on a surface of the steel sheet after a humidity cabinet test lasting 48 hours at a temperature of 50° C. in an atmosphere of 98% humidity, and iron loss W17/50 after the electron beam irradiation is reduced by at least (?500 t2+200 t?6.5) % of the iron loss W17/50 before the electron beam irradiation and is (5 t2?2 t+1.065) W/kg or less.

Abstract: A radiant tubes support device, which can be used in furnaces for the thermal treatment, for continuous lines for galvanising and annealing strips or panels made of metal sheet or other products made of steel or other metals, including a furnace side wall support, constrained to a wall of the furnace, a radiant tube support provided with a tubular element and anti-sticking means between the tubular element and the furnace side wall support for supporting the radiant tube and allowing the lateral oscillation thereof, avoiding the sticking on the furnace side wall support.

Abstract: A cold-rolled steel sheet for vitreous enameling has a predetermined chemical composition, in which a number density of Fe—Mn—Nb-based composite oxides having a diameter of 0.2 ?m to 10 ?m is 2×102 particle/mm2 to 1×104 particle/mm2; a fatigue limit ratio is higher than 0.42 after performing a heat treatment with an applied tensile strain of 10% at a heating temperature of 830° C. for a holding time of 5 minutes; voids are formed between the metallographic structure and the Fe—Mn—Nb-based composite oxides, and an equivalent circle diameter of the voids is 0.1 ?m to 0.6 ?m; and when each of the voids is approximated as a triangle and a long side of the triangle is set as a base, a value obtained by dividing a length of the base by a height of the triangle is 1.0 to 15.

Abstract: This invention provides a hydrometallurgical process for extracting one or more saleable products from a sulphate or chloride pregnant leach solution (PLS), or both. The products may be any one or more of the products selected from the group consisting of: precious metals including platinum group metals (PGMs), gold and silver, base metals, and rare metal elements, and metal cathodes, powders, salts or precipitates thereof; sulphur; hydrochloric acid (HCl); calcium; and silica.

Abstract: A method of producing sodium tungstate of this invention including allowing cemented carbide scrap containing tungsten to react with a molten salt containing sodium sulfate and having added thereto a metal oxide, to thereby obtain sodium tungstate.

Abstract: The present invention relates to a method for producing rare-earth metals (REM) compounds by complex processing of apatite, in particularly to a method for concentrating rare-earth metals (REM) in phosphogypsum, where to a process of decomposition of the REM containing raw phosphate material with sulphuric acid, a sodium salt in the amount of 0.25-5.0 kg in terms of Na2O or a potassium salts in the amount of 0.25-5.0 kg in terms of K2O or a mixture thereof in the amount of 0.25-5 kg in terms of Na2O and K2O to 1 kg of REM (in terms of Ln2O3 present in raw phosphate material) is added. The yield of REM transfer into phosphogypsum is up to 98%.

Abstract: Methods are provided for producing alloy forms from alloys containing one or more extremely reactive elements and for fabricating a component therefrom. The fabricating method comprises substantially removing a reactive gas from the fabrication environment. An alloy form of the alloy is formed. The alloy form is formed by melting the alloy or by melting one or more base elements of the alloy to produce a molten liquid and introducing the one or more extremely reactive elements into the molten liquid. The molten alloy is shaped into the alloy form. The component is formed from the alloy form. If the one or more extremely reactive elements are introduced into the molten liquid, such introduction occurs just prior to the shaping step.

Abstract: Provided are a ferritic/martensitic oxide dispersion strengthened steel with increased high temperature creep resistance, including 0.02 to 0.2 wt % of carbon (C), 8 to 12 wt % of chromium (Cr), 0.1 to 0.5 wt % of yttria (Y2O3), 0.2 to 2 wt % of molybdenum (Mo), 0.01 to 0.5 wt % of titanium (Ti), 0.01 to 1 wt % of manganese (Mn), 0.01 to 0.3 wt % of vanadium (V), 0 to 0.3 wt % of zirconium (Zr), 0 to 0.5 wt % of nickel (Ni), and the remaining content of iron (Fe), and a method of manufacturing the same. The ferritic/martensitic oxide dispersion strengthened steel may be useful as a material for core structural components of a nuclear power system, ultra supercritical pressure steam generator components of a thermal power plant, or engine components of an airplane due to a high tensile strength at 700° C. and excellent creep resistance.

Abstract: There is provided a precipitation hardening stainless steel powder including, in percentage by mass: Si: ?1.0%; Mn: ?1.8%; Ni: 3.0 to 8.5%; Cr: 12.0 to 20.0%; Mo: 0.1 to 2.5%; Cu: 1.0 to 5.0% and/or Ti+Al: 1.0 to 5.0%; Nb+Ta?5C or Nb?5C; N?350 ppm; and the balance being Fe and incidental impurities. A sintered compact fabricated from the steel powder has a martensite content of 90% or more. The precipitation hardening stainless steel powder provides a sintered compact that exhibits high strength after aging.

Abstract: A fabricator resource receives an assembly including a first portion of material and a second portion of material. Initially, the first portion of material in the assembly may have a different modulus of elasticity than the second portion of material. The fabricator resource exposes the assembly to one or more heating/cooling cycles. Exposure of the assembly to the one or more heating/cooling cycles modifies a modulus of elasticity of the first portion of material and a modulus of elasticity of the second portion of material to desired target values (such as substantially same or different values).

Abstract: A method for surface-treating a metallic substrate, in particular steel plate, using a protective coating on a Zn basis, according to which a chloride-containing solution is applied to the protective coating and as a result an anti-corrosion layer comprising hydrozincite and simonkolleite is formed at least in parts. To increase the corrosion resistance of the protective coating and to improve the process sequence and reproducibility of the method, the invention proposes reacting the protectively coated substrate with the solution which, using an acid, is adjusted to a pH of 4 to 6 and contains 1.8 to 18.5% by weight chloride, so as to increase the proportion of simonkolleite in relation to the proportion of hydrozincite in the anti-corrosion layer.

Abstract: The invention relates to a device and a method for hot-dip coating a metal strip with a metal covering, wherein the metal strip is directed continuously through a melt bath, wherein the thickness of the metal covering present on the metal strip when it leaves the melt bath is adjusted by means of a scraping device, and wherein slag which is present on the melt bath is driven away from the metal strip leaving the melt bath by means of a gas flow. To prevent slag from coming into contact with the metal strip leaving the melt bath, the invention drives away the slag from the metal strip by means of at least one nozzle which is arranged in close proximity to the metal strip, that a gas flow which extends over the width of the metal strip is directed onto the surface of the melt bath.

Abstract: A crucible device comprises a crucible body configured to store evaporation material, with an opening disposed therein; a crucible cover which matches the opening of the crucible body and which forms a hermetic space in cooperation with the crucible body when being in a closed state and opens the opening of the crucible body when being in an open state; a driving mechanism comprising a housing with a cavity therein and a piston disposed within the cavity, the cavity being divided by the piston into a closed chamber and an open chamber by the piston, the open chamber being in communication with outside of the cavity through a cavity opening of the cavity, the closed chamber storing gas therein, the piston being connected with the crucible cover by a transmission mechanism, the piston being moved within the cavity in accordance with change of pressure in the open chamber, thereby driving the crucible cover to switch between the open state and the closed state.

Abstract: A deposition apparatus comprises a target unit, an anode unit into which electrons emitted from the target unit flow, a striker configured to come into contact with the target unit to render the target unit and the anode unit conductive, so as to cause arc discharge between the target unit and the anode unit, a striker driving unit configured to drive the striker in one of a direction toward the target unit and a direction to retract from the target unit, a power supply unit configured to supply power to the target unit and the anode unit, and a control unit configured to control the striker driving unit and the power supply unit. The control unit supplies the power to the target unit and the anode unit after bringing the striker into contact with the target unit.

Abstract: A method of coating metallic powder particles includes disposing an amount of metallic powder particles in a fluidizing reactor and removing moisture adhered to the powder particles within the reactor with a working gas at an elevated temperature for a predetermined time. The method further includes coating the powder particles in the reactor with silicon present within the precursor gas at an elevated temperature for a predetermined time and purging the precursor gas from the reactor using the working gas.

Abstract: A vapor deposition method supplies a material gas onto a substrate while heating the substrate by a heater to sequentially form a plurality of films with vapor deposition, grows the film under a constant output control, which keeps an output of the heater at a predetermined output in each of the plurality of films, until a total film thickness of the films formed on the substrate reaches a threshold value, and grows the film under a temperature feedback control, which controls the output of the heater such that a temperature of the substrate measured by a radiation thermometer becomes a predetermined temperature, after the total film thickness reaches the threshold value.

Abstract: A method of fabricating a composite material part, the method including making a consolidated fiber preform, the fibers of the preform being carbon or ceramic fibers and being coated with an interphase; obtaining a consolidated and partially densified fiber preform, the partial densification comprising using chemical vapor infiltration to form a first matrix phase on the interphase; and continuing densification of the fiber preform by infiltrating an infiltration composition containing at least silicon and at least one other element suitable for lowering the melting temperature of the infiltration composition to a temperature less than or equal to 1150° C.

Abstract: Manganese-containing film forming compositions, their preparation, and their use for the vapor deposition of films are disclosed. The manganese-containing film forming compositions comprise silylamide-containing precursors, particularly {Mn[N(SiMe2Et)2]2}2.

Abstract: The invention relates to methods and apparatus in which a plurality of ALD reactors are placed in a pattern in relation to each other, each ALD reactor being configured to receive a batch of substrates for ALD processing, and each ALD reactor comprising a reaction chamber accessible from the top. A plurality of loading sequences is performed with a loading robot. Each loading sequence comprises picking up a substrate holder carrying a batch of substrates in a storage area or shelf, and moving said substrate holder with said batch of substrates into the reaction chamber of the ALD reactor in question.

Abstract: The present invention relates to a facility for the continuous vacuum deposition of a metal coating on a substrate in motion, comprising a vacuum deposition enclosure (24), at least one vapor jet deposition head (25,26) connected to an evaporator pot (9) designed to contain the coating metal in liquid form (11), through a vapor supply pipe (20) provided with a distribution valve (19), and a melting furnace (1) for said metal, said furnace being at atmospheric pressure, located below the lowest portion of the evaporator pot (9) and connected to the evaporator pot (9) by at least one automatic supply pipe (8) of the evaporator pot (9) provided with a supply pump (6) and by at least one liquid metal return pipe (8A,18) optionally provided with a valve (16,17), regulating means for the supply pump (6) further being present to regulate a determined liquid metal level in the evaporator pot (9), characterized in that it comprises, in each said supply and return pipes (8; 8A,18), a so-called heat valve area (7,13,15)