Abstract: The inventive technology relates to novel paratransgenic strategies for the control of pathogens. The inventive technology may specifically include a novel paratransgenic system configured to deliver one or more inhibitory RNA molecules to pathogen/disease-transmitting organisms. In a preferred embodiment, the invention may include one or more genetically engineered enteric bacteria configured to deliver one or more interfering RNA molecules to pathogen/disease-transmitting mosquitos.

Abstract: The present disclosure relates to antisense oligomers and related compositions and methods for inducing exon inclusion as a treatment for glycogen storage disease type II (GSD-II) (also known as Pompe disease, glycogenosis II, acid maltase deficiency (AMD), acid alpha-glucosidase deficiency, and lysosomal alpha-glucosidase deficiency), and more specifically relates to inducing inclusion of exon 2 and thereby restoring levels of enzymatically active acid alpha-glucosidase (GAA) protein encoded by the GAA gene.

Abstract: Process for inducing resistance to diphtheria toxin in a human cell, wherein the process is carried out in vitro and/or in vivo in a rodent xenografted with a human cell, the process comprising: a) providing at least one expression vector containing at least one nucleotide sequence targeting by RNA interference the DPH2 gene transcript of the human cell; c) transducing the at least one vector into the human cell; d) allowing transcription in the human cell, from the at least one expression vector, of the at least one nucleotide sequence targeting by RNA interference the DPH2 gene transcript; e) obtaining silencing of the DPH2 gene in the human cell, whereby the human cell is resistant to diphtheria toxin.

Abstract: The present disclosure provides methods of reducing protein misfolding and/or aggregation in a cell. The present disclosure provides methods of treating diseases and disorders associated with protein misfolding and/or aggregation.

Abstract: The present invention relates to RNAi agents, e.g., double stranded RNAi agents, targeting a xanthine dehydrogenase (XDH) gene, and methods of using such double stranded RNAi agents to inhibit expression of an XDH gene and methods of treating subjects having an XDH-associated disease.

Abstract: The invention relates to a double-stranded ribonucleic acid (dsRNA) for inhibiting the expression of the PCSK9 gene (PCSK9 gene), comprising an antisense strand having a nucleotide sequence which is less that 30 nucleotides in length, generally 19-25 nucleotides in length, and which is substantially complementary to at least a part of the PCSK9 gene. The invention also relates to a pharmaceutical composition comprising the dsRNA together with a pharmaceutically acceptable carrier and method for treating diseases caused by PCSK9 gene expression.

Abstract: The present invention relates to oligomeric compounds and conjugates thereof that target Proprotein Convertase Subtilisin/Kexin type 9 (PCSK9) PCSK9 mRNA in a cell, leading to reduced expression of PCSK9. Reduction of PCSK9 expression is beneficial for a range of medical disorders, such as hypercholesterolemia and related disorders.

Abstract: Described herein are bacterial microcompartments shell proteins modified to stably incorporate iron-sulfur clusters. Such bacterial microcompartments shell proteins exhibit redox cycling and confer electron transfer functionality to bacterial microcompartment shells.

Abstract: Compositions and methods for the inducible expression of genes in eukaryotic cells are provided. Expression of a nucleotide sequence of interest encoding a protein of interest is controlled by a regulatory fusion protein that consists of a transcription blocking domain and a ligand-binding domain. When a cognate ligand for the ligand-binding domain is present, transcription of the nucleotide sequence of interest is blocked, Upon removal of the cognate ligand, the nucleotide sequence of interest is transcribed. The method is useful for large scale bioreactor production of a desired protein of interest in eukaryotic cells.

Abstract: The present invention provides an expression element and an expression cassette to establish a novel vector therefrom. The expression element of the present invention has an effect of enhancing the expression level of the target gene so that is highly industrial valuable. Accordingly, the present vector is taken as a novel tool for genetic engineering.

Abstract: The present disclosure identifies pathways, mechanisms, systems and methods to confer production of carbon-based products of interest, such as sugars, alcohols, chemicals, amino acids, polymers, fatty acids and their derivatives, hydrocarbons, isoprenoids, and intermediates thereof, in engineered and/or evolved methylotrophs such that these organisms efficiently convert C1 compounds, such as formate, formic acid, formaldehyde or methanol, to organic carbon-based products of interest, and in particular the use of organisms for the commercial production of various carbon-based products of interest.

Abstract: A filamentous fungus mutant strain in which enzyme production inhibition caused by glucose is suppressed is constructed, and a method of producing a polysaccharide-degrading enzyme, a method of producing a saccharide from biomass, and a method of saccharifying biomass, each using the filamentous fungus, are provided. The filamentous fungus mutant strain in which Sre1 expression is reduced compared to a parent strain or is lost.

Abstract: The invention provides nucleotide sequences and amino acid sequences of the Dipgene as well as (functional) homologues, fragments and variants thereof, which provides diplospory as a part of apomixis. Also diplospory plants and methods for making these are provided, as are methods of using these, and methods of making apomictic seed.

Abstract: Methods and compositions for Ochrobactrum-mediated transformation of plants are provided. Methods include but are not limited to using an Ochrobactrum strain to transfer a polynucleotide of interest to a plant cell. These include VirD2-dependent methods. Compositions include an Ochrobactrum strain, transfer DNAs, constructs and/or plasmids. These include Ochrobactrum strains having a plasmid comprising one or more virulence gene(s), border region, and/or origin of replication. Plant cells, tissues, plants, and seeds comprising a polynucleotide of interest produced by the methods are also provided.

Abstract: A method of producing plastid transformants including a process (a) in which genes coding for proteins having a function of neutralizing nitrite toxicity are introduced into plant tissues and a process (b) in which the plant tissues into which the genes are inserted in the process (a) are cultured in a medium containing nitrite nitrogen having a concentration at which growth of wild type plant cells is suppressed or a concentration at which regeneration of wild type plants is suppressed and thus plastid transformants obtained by inserting the genes into plastid genomes are screened.

Abstract: The present invention provides methods for enhancing the polyisoprenoid biosynthesis pathway. The present invention further provides isoprenoid-producing plants having an enhanced polyisoprenoid biosynthesis pathway, and methods for producing a polyisoprenoid using such an isoprenoid-producing plant. The present invention relates to methods for regulating the expression of specific protein(s) by a specific transcription factor; isoprenoid-producing plants into which has been introduced a gene encoding a specific transcription factor; and methods for producing a polyisoprenoid using such an isoprenoid-producing plant.

Abstract: A formulation is provided for application to a host plant to reduce, inhibit or impair one or more of growth and development of the host plant. A method of inhibiting growth plant growth and development is also provided as a means of controlling weedy species. The method comprises: selecting a suitable gene for growth suppression in a target plant; identifying an at least one target site accessible to base pairing in the suitable gene; identifying an at least one divergent site in the at least one target site; designing a construct complementary to the at least one divergent site; adding an at least one RNAi inducer to the construct; and delivering the construct to the target plant.

Abstract: Two genes, A622 and NBB1, can be influenced to achieve a decrease of nicotinic alkaloid levels in plants. In particular, suppression of one or both of A622 and NBB1 may be used to decrease nicotine in tobacco plants.

Abstract: Rice is described that is tolerant/resistant to AHAS/ALS inhibitors because of a plurality of mutations that act synergistically in providing resistance/tolerance to the herbicide. Tolerance/resistance is due to presence of combined mutations in the rice leading to amino acid substitutions (A205V and G654E) in the AHAS/ALS enzyme. Use of the rice for weed control and methods of producing tolerant/resistant rice are also disclosed.

Abstract: The invention provides recombinant DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules operably linked to heterologous transcribable DNA molecules, as are methods of their use.

Abstract: This disclosure concerns nucleic acid molecules and methods of use thereof for control of pathogens through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in pathogens. The disclosure also concerns methods for applying dsRNA through formulations and/or transgenic plants that express nucleic acid molecules useful for the control of pathogens, and the plant cells and plants obtained thereby.

Abstract: An artificially synthesized insect-resistant protein, biological materials associated therewith, and use thereof are disclosed. The protein is designated as MRP001 protein, and is a protein of A1) or A2): A1) a protein having an amino acid sequence as shown in SEQ ID No.6; and A2) a protein having insect resistance derived from A1) by substituting, and/or deleting, and/or adding one or more amino acid residues in the amino acid sequence of the protein of A1). It is confirmed through experiments that the MRP001 protein and biological materials associated therewith have resistance to plant bug, and are useful in the preparation of biological ant-insect agent containing the protein, and in the cultivation of insect-resistant transgenic cotton, fruits, tea, rice, vegetables and other crops, thereby reducing the application of pesticides and alleviating the environmental pollution, and thus being of great economic value and broad application prospect.

Abstract: Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest, as probes for the isolation of other homologous (or partially homologous) genes. The insecticidal proteins find use in controlling, inhibiting growth or killing lepidopteran, coleopteran, dipteran, fungal, hemipteran, and nematode pest populations and for producing compositions with insecticidal activity.

Abstract: Use of a rAAV3B vector to deliver gene products to human hepatocytes is described. The rAAV3B vectors achieve high levels of transduction even in the presence of pre-existing immunity to AAV8 or AAVrh10. Compositions and treatment regimens are described. Also provided are rAAV engineered to facilitate purification and methods of purifying the AAV.

Abstract: The present invention relates to one or more promoters and/or expression cassettes that can be used for enhancing expression of a heterologous gene, such as Brachury. In particular, the one or more promoters and/or expression cassettes enhance expression of heterologous genes as part of a viral vector, such as a poxvirus.

Abstract: The present disclosure provides a composition comprising a site-specific nuclease domain capable of cleaving a target DNA sequence; and a sequence-specific DNA binding domain capable of specifically binding to a recognition DNA sequence, wherein the site-specific nuclease domain operably links to the sequence-specific DNA binding domain.

Abstract: The present disclosure is in the field of genome engineering, particularly targeted modification of the genome of a cell.

Abstract: The invention relates to methods and bacterial strains for making terpene and terpenoid products, the bacterial strains having improved carbon pull through the MEP pathway and to a downstream recombinant synthesis pathway.

Abstract: The present invention relates to a novel polypeptide having the enzymatic activity of reduction of NADP+ using electrons from reduced ferredoxin (Ferredoxin-NADP+ reductase activity), a polynucleotide having a nucleotide sequence encoding such polypeptide and uses thereof. The invention relates to the modulation of the Ferredoxin NADP+ reductase activity in a microorganism by varying the expression level of the polynucleotide coding for such polypeptide. The invention also relates to the production of commodity chemicals, especially ethanol, n-butanol, 1,3-propanediol, 1,2-propanediol, isopropanol and acetone by fermenting microorganisms wherein their Ferredoxin NADP+ reductase activity is modulated.

Abstract: The present invention relates to methods for enhancing yield and/or quality of by-products from fermentation processes used for producing fermentation products. The invention includes a process of fermenting starch-containing material into a fermentation product comprising a fermentation step wherein one or more enzymes are added before or during the fermentation step. More specifically, the present invention provides methods for increasing oil recovery yield from processes for producing fermentation products and further enhancing the quality of DDGS by removing fat content from DDGS that are produced as by-products in processes for producing fermentation products.

Abstract: Ear corn is picked from corn fields by ear corn harvesters and transported to a central shelling station associated with an ethanol manufacturing facility. Shelled corn from the central shelling station is processed into ethanol at the ethanol manufacturing facility, and corn cobs from the central shelling station are burned to provide process heat for the ethanol manufacturing process. Energy is conserved and costs are reduced during the picking and shelling of the ear corn and by the burning of cobs for process heat.

Abstract: The present invention provides methods and compositions for increasing lignin degradation to produce a biological product. Also provided are methods for increasing expression of laccase in a bacterial species to produce increased lignin degradation. Also provided are bacterial cells and commodities or commodity produces produced from such methods.

Abstract: The present invention provides a process for obtaining n+a oxidation and reduction products from a mixture of n sugars selected from the group consisting of C5 and C6 sugars, wherein n is at least 2 and a is at least 1, wherein at least two of the sugars in the mixture are present at a non-equimolar ratio to each other, wherein, in a first stage, at least one of the sugars which are present at a non-equimolar ratio to each other is oxidized enzymatically and, at the same time, at least one of the other sugars present at a non-equimolar ratio to each other is reduced enzymatically, and wherein, in the first stage, a portion of at least one of the sugars present at a non-equimolar ratio to each other is not converted, and which is characterized in that, in at least a second stage, at least a portion of the sugar not converted in the first stage is oxidized enzymatically by half and, respectively, is reduced enzymatically by the remaining half.

Abstract: A method for producing a fatty acid ester according to the present invention includes mixing a raw fat or oil, a liquid enzyme, and an alcohol having 1 to 8 carbon atoms in the presence of water and an electrolyte. According to the present invention, a fatty acid ester can be efficiently produced via a transesterification reaction without using any expensive buffer solution or amphipathic substance. Furthermore, in the reaction, it is not necessarily required to agitate reactants at a high speed, which makes the present invention adaptive to various production facilities and conditions.

Abstract: Triglyceride oils having one or more populations of asymmetric triglyceride molecules are provided. Asymmetric triglyceride molecule populations are triglyceride molecules that consist of a C8:0 fatty acid or a C10:0 fatty acid at the sn-1 position and the sn-2 position, and C16:0 or C18:0 at the sn-3 position. Another population of asymmetric triglyceride molecules are triglyceride molecules that consist of a C16:0 fatty acid or a C18:0 fatty acid at the sn-1 position and the sn-2 position, and C8:0 or C10:0 fatty acid at the sn-3 position. Methods of producing triglyceride oils and using the same are provided using sucrose invertase and hydrogenation of the triglyceride oil. Triglyceride molecules are produced by using recombinant DNA techniques to produce oleaginous recombinant cells.

Abstract: This document describes biochemical pathways for producing glutaric acid, 5-aminopentanoic acid, 5-hydroxypentanoic acid, cadaverine or 1,5-pentanediol by forming one or two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C5 backbone substrate such as 2-oxoglutarate.

Abstract: The current disclosure provides a process for enzymatically converting a saccharide into tagatose. The invention also relates to a process for preparing tagatose where the process involves converting fructose 6-phosphate (F6P) to tagatose 6-phosphate (T6P), catalyzed by an epimerase, and converting the T6P to tagatose, catalyzed by a phosphatase.

Abstract: The present invention relates to mutated and/or transformed microorganisms for the synthesis of various compounds. More specifically, the present invention discloses microorganisms mutated in the genes encoding for the regulators ArcA and IclR. The latter mutations result in a significant upregulation of the genes that are part of the colanic acid operon. Hence, said microorganisms are useful for the synthesis of any compound being part of the colanic acid pathway such as GDP-fucose, GDP-mannose and colanic acid, and/or, can be further used—starting form GDP-fucose as a precursor-to synthesize fucosylated oligosaccharides or—starting from GDP-mannose as a precursor—to synthesize mannosylated oligosaccharides. In addition, mutations in the genes coding for the transcriptional regulators ArcA and IclR lead to an acid resistance phenotype in the exponential growth phase allowing the synthesis of pH sensitive molecules or organic acids.

Abstract: Disclosed herein are methods of making composite cellulose hydrogels, the methods comprising providing a cellulose synthesizing microbe; and culturing the cellulose synthesizing microbe in a composition comprising greater than 1% of a cellulose derivative, thereby forming the composite cellulose hydrogel.

Abstract: An arrangement for and a method of biomass hydrolysis includes a feed pump, a pre-hydrolysis reactor and a feed line there-between for feeding fresh biomass slurry to the pre-hydrolysis reactor. The feed pump is a centrifugal feed pump and the pre-hydrolysis reactor is an upflow reactor.

Abstract: The processes disclosed herein include saccharifying cellulosic and/or lignocellulosic biomass and fermenting the sugars to produce a sugar alcohol.

Abstract: This invention discloses multi-part primers for primer-dependent nucleic amplification methods. Also disclosed are primer-dependent nucleic acid amplification reactions, particularly DNA amplification reactions, reaction mixtures and reagent kits for such reactions. This invention relates to primer-dependent nucleic acid amplification reactions, particularly DNA amplification reactions such as PCR, and primers, reaction mixtures and reagent kits for such reactions and assays employing same.

Abstract: The invention relates to a method for producing flavone glycoside dihydrochalcones, having the following steps: (a) providing a transgenic microorganism containing (i) a first nucleic acid portion (A) containing a gene which codes for a bacterial chalcone isomerase and (ii) a second nucleic acid portion (B) containing a gene which codes for a bacterial enoate reductase, (b) adding one or more flavone glycosides to the transgenic microorganism under conditions which allow the simultaneous isomerization and reduction of the flavone glycoside into the flavone glycoside dihydrochalcone, and optionally (d) isolating and purifying the final product, wherein the nucleic acid portion (A) (1) is a nucleotide sequence according to SEQ ID NO:1, in which the nucleic acid portion (A?) according to SEQ ID NO:3 has been cut out, or (2) is an amino acid sequence according to SEQ ID NO:2, in which the amino acid portion (A?) according to SEQ ID NO:4 has been cut out.

Abstract: Vaccines and therapeutic proteins, including polyclonal and monoclonal antibodies, must be maximally pure and stable in their most active native form. This is a requirement for their maximal efficacy, specificity and stability as well as for precluding immune responses against erroneous or damaged moieties. Similar considerations hold for proteins used in diagnostics, industry and research. The most frequent source of damage to proteins produced in living cells is the diverse product of oxidative damage. Two main sources of protein oxidation are the level of reactive oxygen species (ROS) and even more importantly the intrinsic susceptibility of proteins to oxidative damage. Methods for avoiding oxidative protein damage are disclosed, including providing for (i) a decrease in intracellular ROS levels and (ii) an increase in the intrinsic resilience of proteins to oxidative damage.

Abstract: A method of detecting a species, strain or type of bacteria includes mixing a labeled bacteriophage including a label that is detectible via a detection system with a bacterial culture including the species, strain or type of bacteria to which the labeled bacteriophage selectively binds and using the detection system to detect the labeled bacteriophage bound to the species, strain or type of bacteria.

Abstract: Among other things, motility of at least one individual microorganism or a change in motility of at least one individual microorganism or both is or are characterized. The characterized motility or change in motility is used to detect the presence or count of the at least one individual microorganism, or determine the identity of a species or strain of the at least one individual microorganism, or determine a susceptibility of the at least one individual microorganism to one or more antibiotics or other antimicrobials.

Abstract: The present disclosure provides compositions and methods of modulating abhydrolase domain-containing protein-2 (ABHD2). The present disclosure provides methods of identifying agents that modulate ABHD2 activity.

Abstract: The present invention relates to methods for the detection of nucleic acids of defined sequence, and compositions and kits for use in said methods. The methods employ nicking agent(s) and a sequential series of oligonucleotide probes to produce probe fragments in the presence of a target nucleic acid.

Abstract: The present invention provides compositions and methods for colorimetric detection schemes for detecting a variety of biomolecules. The compositions and methods employ DNA hybridization chain reaction for catalytic aggregation of gold nanoparticles. In this catalytic aggregation scheme, a single target DNA strand triggers the formation of multiple inter-particle linkages in contrast to the single linkage formed in conventional direct aggregation schemes.

Abstract: The present invention provides, inter alia, methods and compositions for imaging, at high spatial resolution, targets of interest.