Abstract: A comprehensive two-dimensional gas chromatography system comprising of a sample inlet, a primary dimension column, a secondary dimension column, a thermal modulator, and a detector. The thermal modulator has a first modulation position and a second modulation position. The sample inlet connects to the primary dimension column. The exit of the primary dimension column connects to the first modulation position via a fluidic path, the first modulation position connects to the second modulation position via a fluidic path, the second modulation position connects to the secondary dimension column via a fluidic path, and the exit of the secondary dimension column connects to the detector. The fluidic path between the exit of the primary dimension column and the inlet of the secondary dimension column is a modulation column.

Abstract: The present disclosure relates to the determination of pesticides, e.g., polar pesticides, in a sample using chromatography. The present disclosure can provide direct analysis of polar pesticides, including anionic polar pesticides, using high performance liquid chromatography. The polar pesticides are sufficiently retained and resolved to allow for multiple polar pesticide determinations in a single analysis.

Abstract: A two-dimensional liquid chromatography (2D-LC) apparatus and method of its use, the apparatus constructed with (1) a first dimension (first-D) having at least one first chromatography column, (2) at least one multi-port stream selector in fluid communication with the at least one first chromatography column for receiving an effluent therefrom, and (3) a second dimension (second-D) having a plurality of second chromatography columns, each second chromatography column in fluid communication with a corresponding port of the at least one multi-port stream selector for receiving an effluent fraction of the effluent from the at least one first chromatography column, thereby enabling simultaneous chromatographic separation by the plurality of second chromatography columns of a set of multiple effluent fractions outputted from the at least one first chromatography column.

Abstract: The present invention relates to systems and methods for monitoring agricultural products. In particular, the present invention relates to monitoring fruit production, plant growth, and plant vitality. According to embodiments of the invention, a plant analysis system is configured determine a spectral signature of a plant based on spectral data, and plant color based on photographic data. The spectral signatures and plant color are associated with assembled point cloud data. Morphological data of the plant can be generated based on the assembled point cloud data. A record of the plant can be created that associates the plant with the spectral signature, plant color, spectral data, assembled point cloud data, and morphological data, and stored in a library.

Abstract: An information presentation system according to the present disclosure includes: an analyzer that makes an analysis of food and outputs an analysis value indicating a result of the analysis; a generator that generates information on at least one of food ingestion by a user and calorie consumption by the user based on the analysis value output by the analyzer; and a presentation unit that presents the information generated by the generator. In this way, the information presentation system of the present disclosure can improve display of the analysis result of the food analyzer.

Abstract: Systems, apparatuses, and computer-implemented methods are provided for the real-time quantification of crude oil in an effluent from coreflooding apparatus. Disclosed here is a method of determining the amount of crude oil in an effluent from a coreflooding apparatus by blending the effluent stream with a solvent stream in a mixing device to produce a mixed stream, supplying the mixed stream to an in-line phase separator to produce a first stream containing the solvent and the crude oil from the effluent stream and a second stream containing water and water-miscible components from the effluent stream; and passing the first stream to a continuous flow analyzer to determine the amount of crude oil in the effluent stream.

Abstract: Methods, compositions, and techniques for enhancing the production of hydrocarbons such as crude oil from subterranean hydrocarbon bearing formations. In some embodiments, the invention relates to processes for evaluating enhanced oil recovery mechanisms in carbonate based reservoirs at both the rock-fluid and oil-water interfaces using spectroscopic and interfacial techniques. In further embodiments, the spectroscopic and interfacial techniques include microscopic, rheometric and tensiometric measurements. In preferred embodiments, the disclosed methods and techniques provide reservoir based details at that allow for optimized “smart water” flooding practices and correspondingly higher oil recovery rates.

Abstract: An improved electronic diagnostic device for detecting the presence of an analyte in a fluid sample comprises a casing having a display, a test strip mounted in the casing, a processor mounted in the casing, and a first sensor mounted in the casing and operatively coupled to the processor. The processor is configured to receive a signal from the first sensor when the device is exposed to ambient light thereby causing the device to become activated. The device includes a light shield that exerts pressure across a width of the test strip to prevent fluid channeling along the length of the test strip. The processor is configured to present an early positive test result reading when a measured value exceeds a predetermined early reading threshold value at any time after a predetermined early testing time period.

Abstract: The invention relates to apparatus and methods for apparatus for detecting presence or monitoring profession of a disease in a biological subject, comprising a chamber in which the biological subject passes through, and at least one detection transducer in the chamber; wherein at least two types of information among the chemical composition, cellular classification, and molecular classification of the biological subject, are detected and collected for analysis as to whether the disease is likely to be present or progressing with the biological subject.

Abstract: Melanophilin (MLPH) of the present invention is involved in differentiation into an adipocyte or fat accumulation, and accordingly, obesity can be treated or prevented by inhibiting the MLPH. Further, by measuring an expression level of the MLPH, obesity can be diagnosed and treated, and therapeutic agents for obesity and agents regulating differentiation into adipocytes can be screened.

Abstract: The present embodiments relate to selection of agents effective in reducing or preventing gastrointestinal pain in a subject. Such an agent is selected and identified if it is capable of reducing spontaneous and/or induced transient receptor potential vanilloid 1 (TRPV1) activation.

Abstract: A method is provided capable of testing an immune state in more detail and in a simple and inexpensive manner to identify diseases such as cancer and to identify side-effects of treatment with an anticancer agent and the like, effects of immunotherapy, a state of prognosis, and the like. The method includes: a step of calculating the total number of white blood cells serving as immune cells in a body of a subject and the number of each of immune cell subgroups based on test information on blood counts of blood components and white blood cell images of the subject and analysis information on the CD classification of monoclonal antibodies that bind to surface antigens of white blood cells; and a step of analyzing the immune state of the subject based on a proportion and a change in the numbers of the immune cell subgroups.

Abstract: A biological environment simulating apparatus using rotational force includes a mounting unit on which a cell to he pressurized is mounted, a rotational force application unit configured to apply centripetal force to the mounting unit to make the mounting unit perform a circular movement along a circular path about a predetermined center point and a control unit. The control unit controls the rotational force application unit based on a type of the cell and an appropriate pressure condition matched with the type of the cell so that a pressure satisfying the appropriate pressure condition is applied to the cell.

Abstract: The present invention is in the field of plant breeding and genetics, particularly as it pertains to the genus, Glycine. More specifically, the invention relates to a method for screening soybean plants containing one or more quantitative trait loci for disease resistance, species of Glycine having such loci and methods for breeding for and screening of Glycine with such loci. The invention further relates to the use of exotic germplasm in a breeding program.

Abstract: Provided herein are methods of diagnosing or monitoring the treatment of abnormal glycan accumulation or a disorder associated with abnormal glycan accumulation.

Abstract: Provided are lectenz molecules, which are mutated carbohydrate processing enzyme enzymes that are catalytically inactive and that have had their substrate affinity increased by at least 1.2 fold. Further provided are methods for making and methods of using such lectenz. Further provided are compositions and methods directed to the multiplexed analysis of carbohydrates and carbohydrate containing compounds. The compositions and methods utilize suspension array technology (SAT) and an array of different carbohydrate binding molecules, each carbohydrate binding molecules with a known carbohydrate binding specificity, to obtain a glycoprofile of the carbohydrate structure(s) in a sample. Each carbohydrate binding molecule of a given specificity is linked to the external surface of a population of individually addressable particles.

Abstract: The present disclosure provides methods for the detection of multiple analytes using a single solid phase. The present disclosure also relates to the preparation of solid phases that include receptacles having affixed thereto antibodies directed to at least two different analytes. The methods of the present disclosure can be used, for example, for the quantitative detection of analytes in a sample and the measurement thereof.

Abstract: The present application provides arrays for use in immunosignaturing and quality control of such arrays. Also disclosed are peptide arrays and uses thereof for diagnostics, therapeutics and research.

Abstract: The invention relates to compositions and methods for the immunoassay of an analyte of interest. The analyte is detected in an immunoassay using three or more antibodies, where in each antibody specifically binds to a different epitope on the analyte. When the analyte of interest in a clinical marker for an acute disease, the detection of the analyte by immunoassay is a diagnosis of the occurrence of the disease.

Abstract: The present invention provides a method and a kit for assaying a TSH receptor antibody, which are easy to manipulate and are safe. Specifically, the present invention provides a composition comprising a genetically modified cell forced to co-express a TSH receptor, a cyclic nucleotide responsive calcium channel, and a luminescent protein aequorin. Use of the composition enables the assay of a TSH receptor antibody contained in a sample.

Abstract: A facile laboratory-based method and kit for use in accordance with the method is disclosed. The method allows for the localisation of biomolecules comprising a conjugatable sulfhydryl group to be localised to the surface of cells, such as red blood cells, as lipid conjugates. The method obviates the need to purify the lipid-conjugated biomolecule before contacting with the cells.

Abstract: The invention provides a method for detecting or quantifying creatinine and an immunosuppressive drug selected from tacrolimus and ciclosporin in a blood sample, devices, including lateral flow assay devices, and kits, as well as methods that enables the reduction of nephrotoxicity and/or enables the maintenance of good renal function in an organ or tissue transplant patient who is undergoing treatment with an immunosuppressive drug selected from tacrolimus and ciclosporin, and methods for monitoring nephrotoxicity and/or kidney function in transplant patients.

Abstract: The present invention is directed towards methods for treating non-alcoholic fatty liver disease (NAFLD) in a patient and determining prognosis of NAFLD in a patient.

Abstract: The present invention relates to a method of diagnosing or treating a S100A12:TLR4/MD2/CD14-mediated inflammatory disorder in a subject. In particular, the present invention is related to a compound having binding affinity to hexameric S100A12 complex and its use in diagnosis and therapy.

Abstract: Disclosed are kits and methods for determining the presence or absence of an antibody of interest in a biological sample of a subject. In particular, the methods may detect either pathological or beneficial antibodies. The method may include the step of contacting a biological sample from a subject with a substrate conjugated to an antigen and an Fc receptor operatively linked to a detectable label. Detection of the label may indicate the presence or absence of an antibody of interest.

Abstract: The present inventors identified a subpopulation of genes induced by type I and type II IFNs in a human submandibular gland (HSG) epithelial cell line. Unexpectedly, it was found that the majority of genes that are highly up-regulated by IFN-? are also highly induced by IFN-?. In contrast, there was a substantial group of genes that are highly induced by IFN-? only. In target tissues, this identified subpopulation of genes and probes allow different IFN patterns to be discerned, enabling more precise molecular classification of patient subpopulations. The identified gene probes are useful for selecting and monitoring therapy, and for defining efficacy of novel agents in the autoimmune rheumatic diseases.

Abstract: Definitive diagnosis and early start of treatment cannot be made for tuberculosis since conventional methods for detecting a Mycobacterium tuberculosis complex require a long time plus enormous labor and expense. Because detection is difficult to perform directly from a biological sample, if the biological sample contains no or a very small amount of the Mycobacterium tuberculosis complex-specific secretory protein, there is a risk infection with the Mycobacterium tuberculosis complex will be missed.

Abstract: In this application is described a method for rapidly and accurately identifying B. anthracis in a sample by simultaneously detecting the presence of cell wall antigen and capsule antigen in the same sample culture grown under capsule inducing conditions. Other uses and advantages of the method of the invention are described herein.

Abstract: In various embodiments devices and methods for the detection and/or quantification of clinically relevant pathogens (e.g., bacteria, fungi, viruses, etc.) are provided. In certain embodiments the device comprises a lateral-flow assay that detects the bacterium at a concentration of less than about 6×106 cells/mL, less than about 3×106 cells/ml, less than about 1×106 CFU/mL, or less than about 50 ?g/mL. In certain embodiments the device comprises an aqueous two-phase system (ATPS) comprising a mixed phase solution that separates into a first phase solution and a second phase solution; and a lateral-flow assay (LFA). In certain embodiments the device comprises a flow-through system comprising a concentration component comprising an aqueous two-phase system (ATPS) comprising a mixed phase solution that separates into a first phase solution and a second phase solution; and a detection component disposed beneath said concentration component.

Abstract: The present specification discloses regulatory T cells, compositions including regulatory T cells, methods of identifying, isolating, enriching, obtaining, and/or expanding regulatory T cells or subset populations thereof, kits including components useful for performing such methods, and methods of treating an immune-based disorder in an individual by administering regulatory T cells or compositions comprising such regulatory T cells to an individual in need thereof.

Abstract: The present invention relates to a diagnostic test for the detection of an E7 protein of a human papilloma virus in a biological sample wherein a sandwich ELISA as capture antibody at least two different rabbit monoclonal antibodies which bind to at least two different epitopes are used and as detection antibody at least two different polyclonal anti E7 antibodies are used.

Abstract: Disclosed herein are methods of detecting vascular inflammation associated with acute myocardial infarction and/or prognosticating acute myocardial infarction by detecting a proteolytic fragment of caspase-1 such as the p20 fragment.

Abstract: A method for monitoring health maintenance by collecting an initial blood sample from an individual and dividing the collected blood sample into at least two parts. The first of the two parts is immediately analyzed for multiple blood components levels, and the second part is frozen immediately after the collection to be used at a later time as a comparative standard. After a period of time, a second blood sample is collected from the same individual and analyzed for the same multiple blood components levels in a parallel test with the frozen part. The results obtained from the second blood sample are then compared to the results from the frozen comparative standard to detect real changes in the multiple blood components levels over time.

Abstract: The invention relates to an immunoadsorbent for purifying five kinds of mycotoxins including fumonisin B1 and aflatoxin B1, and a complex affinity column. The immunoadsorbent comprises a solid-phase support, and an anti-fumonisin B1 monoclonal antibody, an anti-aflatoxin B1 monoclonal antibody, an anti-ochratoxin A monoclonal antibody, an anti-zearalenone monoclonal antibody and an anti-sterigmatocystin monoclonal antibody which are coupled to the solid-phase support, wherein the anti-fumonisin B1 monoclonal antibody is secreted by a hybridoma cell strain Fm7A11, and the hybridoma cell strain Fm7A11 has been preserved at China Center for Type Culture Collection, Wuhan University, Wuhan, China on Mar. 29, 2016 with the preservation number of CCTCC No. C201636. The complex affinity column can be used for purification and detection of a fumonisin B1 sample, an aflatoxin B1 sample, an ochratoxin A sample, a zearalenone sample and a sterigmatocystin sample at the same time.

Abstract: A method for rapidly and highly sensitively measuring endotoxin relies on an endotoxin-measuring agent, which includes a factor C derived from Tachypleus tridentatus that does not have His-tag sequence at the C-terminus, a factor B of a horseshoe crab, and a proclotting enzyme of a horseshoe crab.

Abstract: The present disclosure provides systems and methods for the highly sensitive and effective treatment and diagnosis of cancer. The methods disclosed herein take advantage of the discovery of a series of newly identified, inter-chromosomal genetic fusion events that occur upstream from the IGF2BP3 gene, which result in elevated expression of IGF2BP3 protein. The present disclosure utilizes biomarkers developed using this set of newly discovered genetic fusion events and elevated expression of IGF2BP3 protein to not only diagnosis cancer with high sensitivity and reliability, but also to pre-select patient populations that are expected to display an elevated likelihood of success when treated with any of numerous inhibitors of IGF1R-mediated signaling.

Abstract: A unique system for the correlation between removed lymph nodes for lung cancer diagnosis and pathological analysis thereof is provided. Such a system includes the removal of certain lymph nodes from a suspected or known lung cancer patient with subsequent categorization thereof and placement within a properly divided and labeled specimen collection kit. Through the utilization of such a separation and placement allows and facilitates understanding and non-verbal communication between a surgeon and a pathologist in order to denote the location of the removed lymph nodes in relation to a known or suspected lung cancer tumor or growth. The overall diagnostic method, including the important communicative properties accorded both the particular surgeon and pathologist, is encompassed within this invention, as well as the specific collection specimen kit that permits the surgeon proper distinction of specific removal lymph nodes in relation to their location within the patient's body.

Abstract: One or more embodiments of the invention relate to a testing method and kit for testing chronic myelogenous leukemia (CML). One or more embodiments of the invention also relate to a method for isolating tyrosine kinase inhibitor (TKI)-resistant CML cells. One or more embodiments of the invention also relate to an agent for reducing TKI resistance in CML, and to a screening method for the same.

Abstract: Provided herein are methods for measuring a molecular flux rate based on analysis of isotopologue abundance within a mass isotopomer, e.g., using a high resolution mass spectrometric measurement. Such methods may be used, inter alia, to calculate a fraction of newly synthesized target molecules of interest, a replacement rate of target molecules of interest, and/or a rate of breakdown or degradation of target molecules of interest, e.g., based on isotopologue relative abundance.

Abstract: The present invention relates to a reliable method of prediction of lower respiratory tract infection or inflammation in humans, wherein the level of pancreatic stone protein/regenerating protein (PSP/reg) is determined in serum, and a high level is indicative of the development and the severity of the disease, allowing the classification of patients according to risk.

Abstract: The present invention relates to developing customized therapies for a disease or condition in a subject. In particular, the present invention relates to aptamer-based compositions and methods for identifying, modulating and monitoring drug targets in individual with a disease or condition, and further composition and methods for identifying and selecting protein targets for drug development.

Abstract: The present invention is focused on an in vitro method for identifying or screening human subjects at risk of suffering from a pregnancy related disease, in particular from gestational diabetes mellitus or preeclampsia, departing from the level of expression or concentration of a series of biomarkers isolated from a minimally-invasive sample such as gingival crevicular fluid (GCF). Moreover, the method of the invention offers high sensitivity and specificity at an early stage of the pregnancy (see the Examples and figures shown below), which means that it is a strong and cost-effective method for the detection of a pregnancy related disease, in particular gestational diabetes mellitus or preeclampsia, at an early stage of the disease thus procuring effective treatment, avoiding significant long-term adverse effects for both mother and baby.

Abstract: The present disclosure provides methods and reagents useful for analyzing protein-protein interfaces such as interfaces between a presenter protein (e.g., a member of the FKBP family, a member of the cyclophilin family, or PIN1) and a target protein. In some embodiments, the target and/or presenter proteins are intracellular proteins. In some embodiments, the target and/or presenter proteins are mammalian proteins.

Abstract: The present invention is relevant to polypeptides and novel methods of polypeptide evolution. The present invention further relates to methods of identifying a cross-species mutant polypeptide from, or based upon, a template polypeptide.

Abstract: Methods, compositions, and kits for determining whether a subject has non-alcoholic fatty liver disease (NAFLD) are provided. Methods, compositions, and kits for determining whether a subject has non-alcoholic steatosis are also provided. Methods, compositions, and kits for determining whether a subject has non-alcoholic steatohepatitis (NASH) are also provided.

Abstract: The present invention is related to an in vitro method for diagnosing Gaucher's disease in a subject comprising a step of a) detecting a biomarker in a sample from the subject, wherein the biomarker is free lyso-Gb1.

Abstract: Described herein are, inter alia, methods for diagnosing and treating arrhythmogenic cardiomyopathy (ACM) by detecting cardiac intercalated disk proteins, e.g., desmosomal proteins, mechanical and gap junction proteins, in buccal cells. Exemplary desmosomal and gap junction proteins that can be evaluated in the methods described herein include plakoglobin, plakophilin 1, desmoplakin, and Cx43. The methods can also include selecting and/or administering a treatment for ACM to the subject.

Abstract: The application relates to markers for seizures and epilepsy. Polypeptide expression panels or arrays are provided, comprising one or more probes capable of binding specific polypeptides in blood plasma or blood serum of a mammalian subject. Also provided are methods for detecting seizure, methods for predicting seizure, use of sICAM-5 in the treatment of seizure, methods for assessing the effectiveness of a treatment of seizure, and diagnostic kits.

Abstract: Biosensors for small molecules can be used in applications that range from metabolic engineering to orthogonal control of transcription. Biosensors are produced based on a ligand-binding domain (LBD) using a method that, in principle, can be applied for any target molecule. The LBD is fused to either a fluorescent protein or a transcriptional activator and is destabilized by mutation such that the fusion accumulates only in cells containing the target ligand. The power of this method is illustrated by developing biosensors for digoxin and progesterone. Addition of ligand to cells expressing a biosensor activates transcription in yeast, mammalian cells and plants, with a dynamic range of up to about 100-fold or more. The biosensors are used to improve the biotrans-formation of pregnenolone to progesterone in yeast and to regulate CRISPR activity in mammalian cells. This work provides a general methodology to develop biosensors for a broad range of molecules.

Abstract: Disclosed herein is a method for predicting or diagnosing psychiatric disorders through analyzing skin tissues samples minimally invasive or non-invasively collected. The method disclosed herein makes it possible to diagnose psychiatric disorders through objective biomarkers at a very early age, without giving pain to subjects because of noninvasive or minimally invasive feature of skin sample collection method.