Abstract: Methods and devices are provided for simultaneously amplifying a plurality of sample wells for a predetermined amount of amplification, detecting whether amplification has occurred in a first set of the wells, amplifying for an additional amount of amplification and detecting whether amplification has occurred in a second set of the wells. Methods are also provided for analyzing a target nucleic acid sequence using melt curves that were generated in a plurality of amplification cycles.

Abstract: Embodiments of the present invention relate to a method of detecting, identifying, and quantifying microbes in a wastewater treatment process or facility. The invention identifies the microbes by manipulation of their DNA. Microbes are collected, and a portion of the DNA sequence of a particular gene that is found in microbes is amplified. The amplified DNA sequence contains a highly variable region that can be used identify the microbe. Each amplified DNA sequence is matched against known sequences in various microbes. The identity of the microbes present in a sample can be determined and, sometimes, their biological functions too. Also, the relative quantities of the microbes, compared to the total microbial pool, can be determined. With this information, harmful microbes can be identified and targeted for removal from the wastewater, and beneficial microbes can be cultivated as desired.

Abstract: There is provided methods and compositions to diagnose, classify and treat inflammatory bowel disease including ulcerative colitis and Crohn's disease by measuring the levels of certain bacterial taxa and proteins collected from the gut.

Abstract: The present disclosure provides a method for detecting multiple target nucleic acid sequences in a sample using a plurality of IDed double-stranded probes. Each IDed double-stranded probe comprises a double-stranded nucleic acid hybridization probe associated with an IDed substrate. Also provided is a method for determining the sequence of a nucleic acid by using a plurality of IDed double-stranded probes.

Abstract: The application relates to proteome analysis in single cells. Specifically, disclosed are high throughput methods of detecting proteins in single cells using barcoding, aptamers and single cell sequencing. Solid supports used in recording the cell-of-origin of target proteins and target proteins expressed in the cell-of-origin are disclosed. Additionally, methods of detecting proteins and mRNA in single cells are disclosed. Additionally, methods of detecting protein interactions are disclosed. Additionally, methods of detecting post translationally modified proteins in single cells are disclosed. The application also relates to solid supports or beads and methods of producing said solid supports or beads for use in the described methods.

Abstract: This invention provides methods of amplifying genomic DNA to obtain an amplified representative population of genome fragments. Methods are further provided for obtaining amplified genomic DNA representations of a desired complexity. The invention further provides methods for simultaneously detecting large numbers of typable loci for an amplified representative population of genome fragments. Accordingly the methods can be used to genotype individuals on a genome-wide scale.

Abstract: Methods of detecting, probing, mapping and directed sequencing of target nucleic acids are provided using a guide RNA and a Cas9 protein. Methods for detecting the binding of the guide RNA/Cas9 complex to a target nucleic acid where the guide RNA includes a 3? tail sequence that can hybridize to a probe are provided. Methods for detecting the binding of the guide RNA/Cas9 complex to a target nucleic acid where the complex is physically detected are provided.

Abstract: The purpose of the present invention is to provide a method for allowing both sequencing and quantification of a target nucleic acid without performing a ligation reaction and while solving a problem which arises due to PCR amplification efficiency.

Abstract: This invention relates to methods and compositions for detecting a polymorphism in a target nucleic acid sequence using isothermal nucleic acid amplification. More specifically, the present invention relates to using recombinase polymerase amplification (RPA) or Nicking and Extension Amplification Reaction (NEAR) to detect single nucleotide polymorphisms in a target nucleic acid sequence.

Abstract: In one aspect of the present disclosure is a targeted sequencing workflow where an input sample comprising a sufficient quantity of genomic material is provided such minimal or no amplification cycles are utilized prior to sequencing.

Abstract: Provided herein are methods of classifying antigens and epitopes as being recognized by an individual's cellular immune response. More particularly, provided herein are methods for unbiased determination of which antigens are recognized by a population of T cells.

Abstract: This invention relates to a method of identifying mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene, a kit for performing said method, and furthermore to isolated nucleotide sequences being complementary to one or more mutations of the CFTR gene. According to a first aspect of the invention there is provided a method of identifying mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene, including the steps of providing one or more nucleic acid sequences, fully complementary to one or more segments of the CFTR gene, wherein the said one or more nucleic acid sequences correspond to the mutation to be identified; providing a biological sample of an individual to be tested for CF; isolating nucleic acids from the biological sample; and testing the biological sample for the presence of one or more of the nucleic acid sequences using a suitable detection method.

Abstract: The present invention relates to methods for predicting the survival time of patients with decompensated alcoholic cirrhosis. In particular, the present invention relates to a method for predicting the survival time of a patient with decompensated alcoholic cirrhosis comprising i) determining the expression level of OAS2 or MX2 in a sample of peripheral blood mononuclear cells obtained from the patient, ii) comparing the level determined at step i) with a predetermined reference value and iii) and concluding that the patient will have a short survival time when the level determined at step i) is higher than its predetermined reference value or concluding that the patient will have a long survival time when the level determined at step i) is lower than the predetermined reference value.

Abstract: Disclosed are methods, media, and systems for detecting an undiagnosed medical condition by acquiring a plurality of health metrics of the individual, wherein at least one of the plurality of health metrics comprises nucleotide sequence data; implementing a genetic risk rule that defines a genetic risk for the undiagnosed medical condition; implementing a non-genetic risk rule that defines a non-genetic risk for the undiagnosed medical condition; and generating a confidence score for the undiagnosed medical condition that comprises a function of the genetic risk rule and the non-genetic risk rule.

Abstract: Disclosed are methods and kits for evaluating predicting whether an individual IPF has slowly or rapidly progressive IPF.

Abstract: Provided are a method, system and computer readable medium for determining the base information in a predetermined area of a fetus genome, the method comprising following steps: constructing a sequence library for the DNA samples of the fetus genome; sequencing the sequence library to obtain the sequencing result of the fetus, the sequencing result of the fetus comprised of a plurality of sequencing data; and based on the sequencing result of the fetus, determining the base information in the predetermined area according to the hidden Markov model in conjunction with the genetic information of an individual related hereditarily to the fetus.

Abstract: The present disclosure relates to methods for the treatment of multiple myeloma in patients who have one or more cytogenetic alterations. In particular, the disclosure provides methods for treatment of multiple myeloma in patients who have one or more cytogenetic alternations by administering to a patient a treatment regimen comprising a proteasome inhibitor or a pharmaceutically acceptable salt, stereoisomeric or tautomeric form thereof if said patient is identified as a likely responder to the treatment regimen by assessing the presence of said cytogenetic alterations.

Abstract: A biomarker panel of 12 genes based on expression levels. Methods for calculating prognostic scores and patient ranking based on their score and divided into two equal sized cohorts. Kaplan-Meier analysis and a log-rank test were used to determine differences in survival.

Abstract: Novel mutant ESR1 molecules and uses are disclosed.

Abstract: The invention provides methods, systems, and computer readable medium for detecting ploidy of chromosome segments or entire chromosomes, for detecting single nucleotide variants and for detecting both ploidy of chromosome segments and single nucleotide variants. In some aspects, the invention provides methods, systems, and computer readable medium for detecting cancer or a chromosomal abnormality in a gestating fetus.

Abstract: Provided herein is technology for gastrointestinal neoplasia screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of gastrointestinal neoplasm, and classifying the site location of such a gastrointestinal neoplasm (e.g., a colorectal region, a pancreaticobiliary region, a gastroesophageal region).

Abstract: A method for identifying a sub-population within a mixed population of cells is disclosed. The method involves contacting the mixed population of cells with at least one unique binding agent, wherein the at least one unique binding agent is designed to bind to a target molecule present in the sub-population, and wherein the at least one unique binding agent is attached to an epitope specific barcode that represents the identity of the target molecule. The method further involves sequentially attaching two or more assayable polymer subunits to the epitope specific barcode to create unique cell origination barcodes that represent the identities of individual cells to which the at least one unique binding agent has bound; and decoding the epitope specific barcode and cell origination barcodes, thereby identifying the sub-population within the mixed population of cells.

Abstract: Disclosed are methods for identifying microbes that associate with other microbes, and possess new or improved characteristics. Also disclosed are microbes isolated using the methods. Also disclosed are methods for using the microbes.

Abstract: A method of manufacturing a stainless steel pipe includes preparing a hollow shell having a composition (mass %): up to 0.05% C; up to 1.0% Si; 0.01 to 1.0% Mn; up to 0.05% P; below 0.002% S; 0.001 to 0.1% Al; 16.0 to 18.0% Cr; 3.0 to 5.5% Ni; 1.8 to 3.0% Mo; 1.0 to 3.5% Cu; up to 0.05% N; up to 0.05% O; 0 to 0.3% Ti; 0 to 0.3% Nb; 0 to 0.3% V; 0 to 2.0% W; 0 to 0.01% Ca; 0 to 0.01% B; the balance Fe and impurities, holding the shell in a temperature range of 420 to 460° C. for 60 to 180 minutes; and then holding the shell in a temperature range of 550 to 600° C. for 5 to 300 minutes.

Abstract: A method for production of a track bushing for an undercarriage assembly is provided. The method includes carburizing a base material of the track bushing in less than 100 minutes and to a depth of less than 1000 microns. The base material is medium carbon steel. The method includes hardening the base material. The method includes tempering the base material.

Abstract: Examples herein generally relate to sinter blend compositions for use in a sintering process that do not contain coke breeze (0.0% coke breeze), or contain only very small amounts of coke breeze. In particular, these sinter blend compositions are capable of repurposing mixture of iron-making reverts, having high total and metallic iron levels that re-oxidize so as to become a replacement fuel source for the coke breeze typically used in sinter blend compositions for use. in a sintering process, while still managing to produce a sinter with sufficient ISO tumble strengths.

Abstract: Disclosed are coating compositions and methods for their use, comprising 90 wt. % electric arc furnace dust based on the total dry weight of the coating composition. The electric arc furnace dust includes at least 40 wt. % of Fe2O3 and at least 30 wt. % of CaO and CaCO3 combined, based on the total dry weight of the electric arc furnace dust.

Abstract: Disclosed are methods for a simplified process for preparing lithium carbonate from concentrated lithium brine which can be used for battery grade, pharmaceutical and other high purity grade applications. Impure lithium carbonate is precipitated from lithium concentrated brine, preferably lowered in magnesium, subsequently suspended in aqueous solution and reacted with carbon dioxide to form dissolved lithium bicarbonate. The insoluble impurities are filtered and the dissolved boron and metal impurities such calcium, magnesium and iron are physically separated from the lithium bicarbonate solution using ion selective mediums or other selective processes. Lithium carbonate is then subsequently precipitated.

Abstract: Methods for the extraction of cerium and/or thorium from metal compounds and solutions. A single step or two-step extraction method may be applied to selectively precipitate thorium and/or cerium as hydroxides under controlled pH conditions such that a substantially thorium-free and/or cerium-free rare earth element (REE) solution may be formed, such as for the subsequent separation of individual rare earth elements.

Abstract: A method of selectively removing impurities from a scandium-containing feed solution includes contacting an aqueous scandium-containing solution with an organic solvent stream containing an extractant, thereby forming a loaded organic solvent stream containing the impurity or impurities while leaving the scandium in the raffinate. The aqueous stream containing the scandium is washed, diluted and has inorganic salts added before being contacted with a second organic solvent stream to extract the scandium selectively, and followed by stripping the scandium from the scandium-containing loaded organic extractant stream by adding oxalic acid to the loaded organic extractant stream to form scandium oxalate.

Abstract: An electrode is formed of a bismuth brass alloy. The bismuth brass alloy contains about 30 weight percent to about 40 weight percent of zinc, about 1 weight percent to about 10 weight percent of bismuth, and the balance copper. The bismuth brass alloy has a microstructure that includes islands of bismuth dispersed within the base metal formed of copper and zinc and also includes bismuth at the grain boundaries of the base metal. As a large bulky atom with high resistivity against fluorine attack, bismuth segregates at the grain boundaries and blocks the fluorine diffusion into the lattice. In the presence of fluorine, the bismuth brass alloy forms a protective layer on the elongated surface of the body of the electrode. This protective layer inhibits reaction of the base metal formed of copper and zinc with fluorine and thereby preserves the surface of the electrode material.

Abstract: A cold rollable beta titanium alloy is provided by the present disclosure that exhibits excellent tensile strength, and creep and oxidation resistance at elevated temperatures. In one form, the beta titanium alloy includes molybdenum between 13.0 wt. % to 20.0 wt. %, niobium between 2.0 wt. % to 4.0 wt. %, silicon between 0.1 wt. % to 0.4 wt. %, aluminum between 3.0 wt. % to 5.0 wt. %, zirconium greater than 0.0 wt. % and up to 3.0 wt. %, tin up to 5.0 wt. %, oxygen up to 0.25 wt. %, and a balance of titanium and incidental impurities. Additionally, the ranges for each element satisfies the conditions of: 6.0 wt. %?X wt. %?7.5 wt. %; and??(i) 3.5 wt. %?Y wt. %?5.15 wt. %, where??(ii) X wt. %=aluminum+tin/3+zirconium/6+10*(oxygen+nitrogen+carbon), and Y wt. %=aluminum+silicon*(zirconium+tin).

Abstract: Solid immiscible alloys and methods for making the solid immiscible alloys are provided. The microstructure of the immiscible alloys is characterized by a minority phase comprising a plurality of particles of an inorganic material dispersed in a majority phase comprising a continuous matrix of another inorganic material. The methods utilize nanoparticles to control both the collisional growth and the diffusional growth of the minority phase particles in the matrix during the formation of the alloy microstructure.

Abstract: An aluminum alloy material includes zinc whose mass percentage is from 4.5% to 12.0%, magnesium whose mass percentage is from 0.7% to 3.0%, copper whose mass percentage is less than or equal to 0.6%, titanium whose mass percentage is from 0.001% to 0.5%, boron whose mass percentage is from 0.00011% to 0.2%, manganese whose mass percentage is less than or equal to 0.01%, chromium whose mass percentage is less than or equal to 0.2%, zirconium whose mass percentage is less than or equal to 0.2%, silicon whose mass percentage is less than or equal to 0.3%, ferrum whose mass percentage is less than or equal to 0.3%, aluminum, and other inevitable impurities.

Abstract: An embodiment of a superalloy composition includes 1.5 to 4.5 wt % Al; 0.005 to 0.06 wt % B; 0.02 to 0.07 wt % C; 21.0 to 26.0 wt % Co; 11.5 to 16.0 wt % Cr; 8.50 to 19.0 wt % Ta; 0.005-0.10 wt % Zr; and balance Ni and incidental impurities.

Abstract: A steel wire rod for a spring includes: C: 0.2 to 1.2%, Si: 1.0 to 3%, Mn: 0.1 to 2%, Cr: 3% or less (not inclusive of 0%), Al: 0.0002 to 0.005%, Ca: 0.0002 to 0.002%, Ti: 0.0003 to 0.010%, and balance: iron and unavoidable impurities. An average composition of oxide-based inclusions having a minor axis of 1 ?m or more which are present in a cross section satisfies CaO: 35% or less, Al2O3: 40% or less, SiO2: 30 to 95%, MgO: 8% or less, MnO: 5% or less, TiO2: 3 to 10%, and CaO+Al2O3+SiO2+MgO+MnO+TiO2?80%. The number of oxide-based inclusions having a minor axis of 2 ?m or more which are present in the cross section is more than 0.002 inclusions/mm2.

Abstract: A precipitation hardenable, martensitic stainless steel alloy is disclosed. The alloy has the following composition in weight percent, about C 0.03 max Mn 1.0 max Si 0.75 max P 0.040 max S 0.020 max Cr 10-13 Ni 10.5-11.6 Mo 0.25-1.5? Co 0.5-1.5 Cu 0.75 max Ti 1.5-1.8 Al 0.3-0.8 Nb 0.3-0.8 B 0.010 max N 0.030 max The balance is iron and usual impurities. The disclosed alloy provides a unique combination of corrosion resistance, strength, and toughness.

Abstract: Disclosed is, as a high-strength steel plate of API X80 grade or higher with a thickness of 38 mm or more, a thick steel plate for structural pipes or tubes that exhibits high strength in the rolling direction and excellent Charpy properties at its mid-thickness part without addition of large amounts of alloying elements. The thick steel plate for structural pipes or tubes disclosed herein has: a specific chemical composition; a microstructure at its mid-thickness part that is a dual-phase microstructure of ferrite and bainite with an area fraction of the ferrite being less than 50%, and that contains ferrite grains with a grain size of 15 ?m or less in an area fraction of 80% or more with respect to the whole area of the ferrite; a tensile strength of 620 MPa or more; and a Charpy absorption energy vE?20+ C. at ?20° C. at the mid-thickness part of 100 J or more.

Abstract: A stainless steel alloy comprising essentially 19 to 22 percent by weight chromium, 8.5 to 10.5 percent by weight nickel, 4.5 to 5.75 percent by weight silicon, 0.8 to 2.2 percent by weight carbon, 0.0 to 0.2 percent by weight nitrogen, 0.2 to 1.2 percent by weight cobalt, 3.0 to 7.0 percent by weight manganese, 0.0 to 9.0 percent by weight niobium, 0.005 to 0.6 percent by weight titanium, 0.3 to 6.0 percent by weight molybdenum, and the balance iron plus impurities.

Abstract: A multiple step method increases net tensile strengths of high pressure die cast (HPDC) aluminum components through an alloy- and process-dependent thermal treatment. The highest temperature feasible for solution treatment of an HPDC casting is determined by computational thermodynamics, kinetics and the gas laws based on the alloy composition and gas pressure in the finally solidified parts. Determining the maximum solution temperature involves mapping pressure in the bubbles of solidified material to avoid the formation of blisters by surface adjacent bubbles in the casting. To reduce residual tensile stress, the HPDC parts are air cooled after the solution treatment. Finally, a specific, multiple temperature aging cycle is utilized to improve the aging response of air cooled HPDC parts and increase net tensile strength.

Abstract: Provided are a zinc alloy plated steel sheet and a method for manufacturing the zinc alloy plated steel sheet. The zinc alloy plated steel sheet includes a base steel sheet and a zinc alloy plating layer, wherein the zinc alloy plating layer includes a Zn single phase structure as a microstructure and a Zn—Al—Mg-based intermetallic compound, and the Zn single phase structure has a degree (f) of (0001) preferred orientation expressed by Formula 1 below within a range of 50% or greater. [Formula 1] f(%)=(Ibasal/Itotal)×100 where Itotal refers to the integral of all diffraction peaks of the Zn single phase structure when an X-ray diffraction pattern is measured within a range of 2 theta from 10° to 100° using a Cu-K? source, and Ibasal refers to the integral of diffraction peaks of the Zn single phase relating to a basal plane.

Abstract: A low temperature carburizing method according to the present invention comprises: step (a) for pre-processing a metal to be processed; step (b) for inputting the metal to be processed to a reaction chamber and heating the same to a set temperature; step (c) for forming a vacuum atmosphere in the reaction chamber and introducing a reaction gas thereinto at a predetermined pressure to accelerate carburization; step (d) for supplying the reaction gas to the reaction chamber at a pressure equal to or lower than the pressure of the reaction gas of step (c) to spread carburization; and step (e) for repeating step (c) and step (d) at predetermined time intervals.

Abstract: A method for producing a case-hardened martensitic stainless steel article includes: providing an article comprised, at least in part, of a martensitic stainless steel, carburizing the article within a temperature range of 1625° F.-1680° F. (885° C.-916° C.), and then carbo-nitriding the article within a temperature range of 1575° F.-1625° F. (857° C.-885° C.). An article, such as a bearing ring, comprising such a case-hardened martensitic stainless steel is also disclosed.

Abstract: Methods, systems, and apparatus for fabricating a physical vapor deposition (PVD) mandrel-target assembly are provided. One method includes providing a mandrel and a PVD target and placing a hollow center portion of the PVD target around a portion of the mandrel such that the portion of the mandrel resides in the hollow portion to create a PVD mandrel-target assembly including space between an exterior surface of the mandrel and an interior surface of the PVD target. The method further includes heating an interior surface of the mandrel, an exterior surface of the PVD target, and an adhesive material to a predetermined temperature and filling the space with the adhesive material to connect the exterior surface of the mandrel and the interior surface of the PVD target. Systems and apparatus for performing the above method are also provided.

Abstract: The present disclosure provides a holding arrangement. The holding arrangement for holding a substrate includes: a body portion having a first side; a dry adhesive material provided on the first side of the body portion; a seal surrounding the dry adhesive material and configured to provide a vacuum region on the first side, wherein the dry adhesive material is provided in the vacuum region; and a conduit to evacuate the vacuum region.

Abstract: The present invention is in the field of processes for the generation of thin inorganic films on substrates, in particular atomic layer deposition processes. The present invention relates to a process comprising bringing a compound of general formula (I) into the gaseous or aerosol state and depositing the compound of general formula (I) from the gaseous or aerosol state onto a solid substrate, wherein R1, R2, R3, and R4 are independent of each other an alkyl group, an aryl group or a trialkylsilyl group, M is Mn, Ni or Co, X is a ligand which coordinates M, wherein at least one X is a neutrally charged ligand, m is 1, 2 or 3 and n is at least 1 wherein the molecular weight of the compound of general formula (I) is up to 1000 g/mol.

Abstract: A reactor device for chemical vapor deposition includes a reaction chamber having a side wall and a substrate stand having a peripheral surface and a main surface facing a reactive gas injector, the injector and said surface defining a work space therebetween. The substrate stand is arranged in the reaction chamber such as to form an annular passage between the peripheral surface of the substrate stand and the side wall of the reaction chamber. A system for discharging gases is in fluid connection with the reaction chamber. A purge gas injector includes an injection channel leading into the reaction chamber through an annular opening. A laminar flow of purge gas is injected through the annular opening and flows in said annular passage to an opening.

Abstract: A gas injection apparatus, which can sequentially supply a substrate with at least two kinds of source gases reacting with each other in a container, and thin film deposition equipment including the gas injection apparatus, are provided. The gas injection apparatus includes a base plate, a first gas supply region protruding from the base plate, a second gas supply region protruding from the base plate and adjacent the first gas supply region, and a trench defined by a sidewall of the first gas supply region and a sidewall of the second gas supply region. The sidewall of the first gas supply region and the sidewall of the second gas supply region face each other and extend in a radial direction on the base plate.

Abstract: The aim of the present invention lies in inexpensively providing a thin-film laminated film having excellent barrier properties. A further aim of the present invention lies in providing a method for producing a thin-film laminated film in which a plurality of thin films are coated on a plastic film by means of atmospheric-pressure plasma CVD which has low equipment costs and running costs, and also in providing a thin-film laminated film production apparatus which is able to operate continuously for a long period of time and can produce high-speed coating. A thin film according to the present invention includes a plastic film, a first silicon oxide-based thin film containing silicon oxide as the main component, and a first amorphous carbon-based thin film. The first amorphous carbon-based thin film is formed on the first silicon oxide-based thin film. The first silicon oxide-based thin film has pinholes with a diameter in the range of 10-200 nm.

Abstract: A method for providing composite phosphate conversion crystal coating, including the steps of: mixing powdered materials with metal oxide particles, thereby providing a composite metal oxide powder including particles of at least one additional powdered material, the at least one additional powdered material providing additional functionality to the phosphate conversion crystal coating; pretreating a substrate by depositing particles of the composite metal oxide powder on the substrate; treating the substrate with a phosphate coating solution, resulting in the composite phosphate conversion crystal coating forming on the substrate which contains fixed particles of the at least one additional powdered material.