Patents Issued in December 11, 2018
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Patent number: 10150947Abstract: In accordance with the purpose(s) of the present disclosure, as embodied and broadly described herein, embodiments of the present disclosure, in one aspect, relate to methods of making a structure including nanotubes, a structure including nanotubes, methods of delivering a fluid to a cell, methods of removing a fluid to a cell, methods of accessing intracellular space, and the like.Type: GrantFiled: January 8, 2016Date of Patent: December 11, 2018Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Jules J. Vandersarl, Alexander M. Xu, Nicholas A. Melosh, Noureddine Tayebi
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Patent number: 10150948Abstract: The present disclosure provides compositions comprising a hydrogel and a cell adhesion ligand that enhances the differentiation of mesenchymal stem cells into brown adipocytes, and/or enhances the maintenance of brown adipocytes. In some cases, a subject composition includes cells that are embedded within the hydrogel. The present disclosure also provides methods of making and using the subject compositions.Type: GrantFiled: December 10, 2014Date of Patent: December 11, 2018Assignee: The Regents of the University of CaliforniaInventors: Andreas Stahl, Kevin Tharp, Kevin E. Healy, Amit Kumar Jha, Judith Kraiczy
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Patent number: 10150949Abstract: The present invention provides a method for producing or detecting cardiomyocytes by extracting/detecting cardiomyocytes from a cell population which includes cardiomyocytes using, as an index, positivity of NCAM1, SSEA3, SSEA4 and/or CD340.Type: GrantFiled: June 12, 2017Date of Patent: December 11, 2018Assignee: Kyoto UniversityInventors: Shinya Yamanaka, Yoshinori Yoshida, Shunsuke Funakoshi
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Patent number: 10150950Abstract: A method for culturing mesenchymal stem cells. The method includes isolating mesenchymal stem cells having a size of 8 ?m or less and culturing the mesenchymal stem cells in a medium containing calcium and magnesium and under a low oxygen condition. The method can significantly improve the proliferative capacity and differentiation potential of the mesenchymal stem cells.Type: GrantFiled: August 29, 2014Date of Patent: December 11, 2018Assignee: MEDIPOST CO., LTDInventors: Yoon-Sun Yang, Won Il Oh, Hye Jin Jin, Soon-Jae Kwon, Miyeon Kim
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Patent number: 10150951Abstract: A method is provided for making a banana or plantain product comprising providing at least one unpeeled banana or plantain comprising banana or plantain peel and banana or plantain pulp, subjecting the at least one unpeeled banana or plantain to a heat treatment at a temperature and for a time sufficient to gelatinize starch present in the at least one unpeeled banana or plantain to form at least one heat treated unpeeled banana or plantain, and comminuting the at least one heat treated unpeeled banana or plantain to form a banana or plantain puree. A functional food ingredient is also provided comprising a banana or plantain puree including banana or plantain pulp and optionally banana or plantain peel. Foods containing banana or plantain puree or powder are provided, including crackers, snack bars, cereals, smoothies, and cookies.Type: GrantFiled: December 16, 2013Date of Patent: December 11, 2018Assignee: Dow Global Technologies LLCInventors: Shang-Tian Yang, Ehab Ammar, Christopher C. Stowers, Brandon A. Rodriguez
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Patent number: 10150952Abstract: Described herein are oligosaccharyl transferases for use in N-glycosylating proteins of interest in vitro and in host cells. Methods for using such oligosaccharyl transferases, nucleic acids encoding such oligosaccharyl transferases, and host cells comprising such oligosaccharyl transferases are also provided herein. Glycoconjugates generated by using such oligosaccharyl transferases are also provided herein.Type: GrantFiled: December 24, 2015Date of Patent: December 11, 2018Assignee: GLAXOSMITHKLINE BIOLOGICALS S.A.Inventors: Jurgen Haas, Julian Ihssen, Michael Thomas Kowarik, Torsten Franz Schwede, Linda Christiane Thöny-Meyer
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Patent number: 10150953Abstract: The present invention relates to a method for preparing an expression vector encoding a well-tolerated and highly specific tailored recombinase, which tailored recombinase is capable of recombining asymmetric target sequences within the long terminal repeat (LTR) of proviral DNA of a plurality of retrovirus strains which may be inserted into the genome of a host cell, as well as to the obtained expression vector, cells transfected with these, expressed recombinase and pharmaceutical compositions comprising the expression vector, cells and/or recombinase. Pharmaceutical compositions are useful, e.g., in treatment and/or prevention of retrovirus infection, in particular, HIV infection. In particular, the invention relates to well-tolerated and highly specific tailored recombinases capable of combining asymmetric target sequences in a more than 90% of HIV-strains, thereby excising the HIV-1 sequences, and expression vectors encoding them.Type: GrantFiled: September 1, 2015Date of Patent: December 11, 2018Assignees: Heinrich-Pette-Institut Leibniz-Institut für Experimentelle Virologie-Stiftung bürgerlichen Rechts, Technische Universität DresdenInventors: Joachim Hauber, Jan Chemnitz, Frank Buchholz, Janet Karpinski
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Patent number: 10150954Abstract: Presented herein are polymerase enzymes for improved incorporation of nucleotide analogs, in particular nucleotides which are modified at the 3? sugar hydroxyl, as well as methods and kits using the same.Type: GrantFiled: June 1, 2017Date of Patent: December 11, 2018Assignee: ILLUMINA, INC.Inventors: Erin Bomati, Michael Previte, Matthew William Kellinger, Cheng-Yao Chen, Molly He
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Patent number: 10150955Abstract: Stabilized reverse transcriptase fusion proteins including a thermostable reverse transcriptase connected to a stabilizer protein are described. Attaching the stabilizer protein to the thermostable reverse transcriptase stabilizes the fusion protein and can aid in its purification, provide increased solubility, allow for longer storage, or allow the fusion protein to be used under more rigorous conditions such as higher temperature. The stabilized reverse transcriptase fusion protein can also include a linker between the stabilizer protein and the thermostable reverse temperature. The stabilized reverse transcriptase fusion proteins are suitable for use in nucleic acid amplification methods such as the reverse transcription polymerase chain reaction and other applications involving cDNA synthesis.Type: GrantFiled: May 25, 2016Date of Patent: December 11, 2018Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEMInventors: Alan M. Lambowitz, Sabine Mohr, Georg Mohr, Eman Ghanem
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Patent number: 10150956Abstract: Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.Type: GrantFiled: January 10, 2018Date of Patent: December 11, 2018Assignees: E I DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED IINTERNATIONAL, INC.Inventors: Ericka Bermudez, Andrew Mark Cigan, James J English, Saverio Carl Falco, Huirong Gao, Lu Liu, Zhan-Bin Liu, Azalea S Ong, Sergei Svitashev, Joshua K Young
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Patent number: 10150957Abstract: The present invention relates to protease variants and methods for obtaining protease variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: GrantFiled: July 28, 2014Date of Patent: December 11, 2018Assignee: Novozymes A/SInventors: Frank Winther Rasmussen, Rolf Thomas Lenhard, Miguel Duarte Guilherme Perreira Toscano, Esben Peter Friis, Signe Eskildsen Larsen, Jurgen Carsten Franz Knotzel, Mikael Bauer
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Patent number: 10150958Abstract: Provided are compositions comprising newly identified protein fragments of aminoacyl-tRNA synthetases, polynucleotides that encode them and complements thereof, related agents, and methods of use thereof in diagnostic, drug discovery, research, and therapeutic applications.Type: GrantFiled: January 12, 2017Date of Patent: December 11, 2018Assignees: aTyr Pharma, Inc., Pangu Biopharma LimitedInventors: Leslie Ann Greene, Kyle P. Chiang, Fei Hong, Alain P. Vasserot, Wing-Sze Lo, Jeffry D. Watkins, Cheryl L. Quinn, John D. Mendlein
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Patent number: 10150959Abstract: The invention is directed to immobilized ketoreductases and methods of making and using them. Enzymes are protein molecules which serve to accelerate the chemical reactions of living cells (often by several orders of magnitude). Without enzymes, most biochemical reactions would be too slow to even carry out life processes. Enzymes display great specificity and are not permanently modified by their participation in reactions. Since they are not changed during the reactions, enzymes can be cost effectively used as catalysts for a desired chemical transformation.Type: GrantFiled: March 12, 2014Date of Patent: December 11, 2018Assignee: Merck Sharp & Dohme Corp.Inventors: Matthew D. Truppo, Hongmei Li, Johannah R. Moncecchi, Hallena Strotman
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Patent number: 10150960Abstract: Provided herein is a method for enriching a target nucleic acid molecule. In one embodiment, the method may involve hybridizing a C-probe to a strand of a target nucleic acid to produce a complex, enzymatically removing any 3? overhanging end from the target nucleic acid of the complex to produce a 3? hydroxyl group at the 3? end; extending the 3? end of the first sequence using the oligonucleotide sequence of the C-probe as a template; enzymatically removing any 5? overhanging end from the target nucleic acid, either before or after the extending step, to produce an 5? phosphate group at the end of the second sequence; and ligating the 5? phosphate group at the end of the second sequence to the 3? hydroxyl group at the end of the first sequence to produce a circular DNA molecule that contains the target sequence and the complement of the oligonucleotide sequence.Type: GrantFiled: February 26, 2013Date of Patent: December 11, 2018Assignee: AGILENT TECHNOLOGIES, INC.Inventor: Brian Jon Peter
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Patent number: 10150961Abstract: The present disclosure relates to a method for producing a monoclonal antibody that binds to an epitope for the glycan binding site of a glycoprotein. According to the present disclosure, a monoclonal antibody that recognizes the glycan binding site of a glycoprotein as an epitope can be produced in a relatively simple and economic manner, and a monoclonal antibody having high sensitivity and specificity for the glycan binding site of a glycoprotein can be produced. The monoclonal antibody produced according to the present disclosure can be effectively used as an agent for diagnosing or treating various diseases associated with a change in glycan structure or glycan expression level.Type: GrantFiled: July 19, 2013Date of Patent: December 11, 2018Assignees: Seoul National University R&DB Foundation, Kangwon National University Industry Cooperation FoundationInventors: Eugene C. Yi, Min Jueng Kang, Kristine M. Kim, Eunhee G. Kim
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Patent number: 10150962Abstract: This application provides an improved screening method for the selection of target-binding proteins having desirable biophysical properties. The method combines mRNA display and yeast surface display in a way that takes advantage of the desirable attributes of both processes.Type: GrantFiled: April 29, 2016Date of Patent: December 11, 2018Assignee: Bristol-Myers Squibb CompanyInventor: Dasa Lipovsek
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Patent number: 10150963Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.Type: GrantFiled: March 27, 2017Date of Patent: December 11, 2018Assignee: 10X GENOMICS, INC.Inventors: Benjamin Hindson, Serge Saxonov, Kevin Ness, Paul Hardenbol, Christopher Hindson, Donald Masquelier, Mirna Jarosz, Michael Schnall-Levin
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Patent number: 10150964Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.Type: GrantFiled: August 31, 2017Date of Patent: December 11, 2018Assignee: 10X GENOMICS, INC.Inventors: Benjamin Hindson, Serge Saxonov, Kevin Ness, Paul Hardenbol, Christopher Hindson, Donald Masquelier, Mirna Jarosz, Michael Schnall-Levin
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Patent number: 10150965Abstract: This invention relates to compounds, compositions, and methods useful for reducing transthyretin (TTR) target RNA and protein levels via use of dsRNAs, e.g., Dicer substrate siRNA (DsiRNA) agents.Type: GrantFiled: December 5, 2014Date of Patent: December 11, 2018Assignee: Dicerna Pharmaceuticals, Inc.Inventors: Bob D. Brown, Henryk T. Dudek
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Patent number: 10150966Abstract: Provided is a novel useful microRNA having improved anti-tumor activity, obtained by introducing a variation in a microRNA which is present in-vivo and which exhibits an anti-tumor effect. This microRNA containing a base sequence (SEQ ID NO: 1) obtained by varying a predetermined region of the base sequence of miR-29b is able to exhibit a particularly outstanding anti-tumor effect.Type: GrantFiled: March 4, 2015Date of Patent: December 11, 2018Inventors: Hirofumi Yamamoto, Masaki Mori
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Patent number: 10150967Abstract: Described herein are compositions and methods for the inhibition of miR-122 activity. The compositions have certain nucleoside modifications that yield potent inhibitors of miR-122 activity. The compounds may comprise conjugates to facilitate delivery to the liver. The compositions may be administered to subjects infected with hepatitis C virus, as a treatment for hepatitis C virus and related conditions.Type: GrantFiled: January 11, 2017Date of Patent: December 11, 2018Assignee: Regulus Therapeutics Inc.Inventors: Balkrishen Bhat, Daniel Hogan
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Patent number: 10150968Abstract: Methods for producing heterologous multi-subunit proteins in transformed cells are disclosed. In particular, the present disclosure provides improved methods of producing multi-subunit proteins, including antibodies and other multi-subunit proteins, which may or may not be secreted, with a higher yield and decreased production of undesired side-products. In exemplary embodiments, the transformed cells are a yeast, e.g., methylotrophic yeast such as Pichia pastoris.Type: GrantFiled: August 20, 2012Date of Patent: December 11, 2018Assignee: ALDERBIO HOLDINGS LLCInventors: Danielle Marie Mitchell, Leon F. Garcia-Martinez, Patricia Dianne McNeill, Ethan Wayne Ojala, Mehmet Inan, John Latham
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Patent number: 10150969Abstract: A genetic screen for Arabidopsis mutants displaying a hyper-susceptible to Agrobacterium transformation (hat) phenotype was performed. The gene disrupted in the hat3 mutant encodes a putative myb-family transcription factor (MTF) that negatively regulates susceptibility to Agrobacterium-mediated transformation. Over-expression of an integrin-like protein results in plants that are hyper-susceptible to transformation. Manipulation of MTF, members of this protein family, and members of the integrin domain-like protein family for example At14a allows improved control of Agrobacterium transformation, including in crops.Type: GrantFiled: January 22, 2016Date of Patent: December 11, 2018Assignee: Purdue Research FoundationInventors: Stanton B. Gelvin, Nagesh Sardesai
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Patent number: 10150970Abstract: Increased antigenicity of a membrane-bound polypeptide produced from a plant is provided in a process in which extraction of the polypeptide or other compounds from the plant is such that phospholipids are associated with the polypeptide. Reducing fat by supercritical fluid extraction increases antigenicity of such plant-produced membrane-bound polypeptides. Methods and means of producing such membrane-bound polypeptides are provided. Methods to produce a protective response in animals are provided by administering to the animal the membrane-bound polypeptide. Binding of antibody specific to the membrane-bound polypeptide is increased. The process provides for increased preferred formation of the membrane-bound polypeptide. Stability of the membrane-bound polypeptide is increased when the plant material is defatted.Type: GrantFiled: March 6, 2014Date of Patent: December 11, 2018Assignee: Applied Biotechnology Institute, Inc.Inventors: John Howard, Celine Hayden, Rafael Jimenez-Flores
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Patent number: 10150971Abstract: The present invention relates to isolated nucleic acid molecules and their corresponding encoded polypeptides. The present invention further relates to the uses of these nucleic acid molecules and polypeptides. For example, the nucleic acid molecules and polypeptides could be used in making enzymes or used to make plants, plant cells, plant materials or seeds of a plant having such modulated growth or phenotype characteristics that are altered with respect to wild type plants grown under similar conditions.Type: GrantFiled: March 7, 2016Date of Patent: December 11, 2018Assignee: CERES, INC.Inventors: Vyacheslav Brover, Timothy J. Swaller, Kenneth A. Feldmann, Maxim Troukhan
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Patent number: 10150972Abstract: The present invention provides a composition for enhancing immunity. Differing from all commercial vitamin healthcare foods would cause some adverse effects due to excessive vitamin intake, the present invention proposes a Solanum nigrum-fermented product to be a novel composition for enhancing immunity. Moreover, animal experimental results have proved that, this novel composition would not cause any adverse effects (such as poor appetite or liver damage) even if an adult excessively administer the novel composition. In addition, comparing with the commercial healthcare foods always being made to tablets, the Solanum nigrum-fermented product (i.e., the novel composition) can be processed to various forms, such as powder, tablet, capsule, and drink.Type: GrantFiled: December 20, 2016Date of Patent: December 11, 2018Assignee: TAIWAN INDIGENA BOTANICA CO., LTD.Inventors: Ching-Hua Su, Cheng-Jeng Tai, Bao-Hong Lee, Chen-Jei Tai, Yeu-Ching Shi
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Patent number: 10150973Abstract: The present invention relates to an apparatus and a method for fermenting, separating, and refining a product, which is produced by cultivating a microorganism. The apparatus and the method for fermenting, separating, and refining, of the present invention, can separate and refine the product that is produced by microbial fermentation in a simple, continuous manner and with high efficiency.Type: GrantFiled: April 13, 2012Date of Patent: December 11, 2018Assignee: GS CALTEX CORPORATIONInventors: Sang-Hyun Lee, Moon-Ho Eom, Julia Lee, Sang-Jun Jeon, Jung-Hee Cho, Jin Dal Rae Choi
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Patent number: 10150974Abstract: The invention relates to a process and system for the production of a solvent using monophasic solventogenic Clostridia in a single stage process. More particularly, the process and system relate to the use of a culture vessel for culturing monophasic solventogenic Clostridia, wherein cell growth of the Clostridium is monitored and optimized by the addition of controlled amounts of culture media and/or the removal of controlled amounts of solvent. The process and system may particularly be used to produce solvents such as butanol, acetone and ethanol.Type: GrantFiled: June 27, 2014Date of Patent: December 11, 2018Assignee: GREEN BIOLOGICS LIMITEDInventors: Edward Green, Ross Patrick Simms, Carl-Axel Magnusson Lalander, Rosa Maria Dominguez-Espinosa
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Patent number: 10150975Abstract: Compositions and methods for producing aldehydes, alkanes, and alkenes are described herein. The aldehydes, alkanes, and alkenes can be used in biofuels.Type: GrantFiled: October 4, 2016Date of Patent: December 11, 2018Assignee: REG LIFE SCIENCES, LLCInventors: Andreas W. Schirmer, Mathew A. Rude, Shane A. Brubaker
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Patent number: 10150976Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as adipate, 6-aminocaproate, hexamethylenediamine or caprolactam. Also provided herein are methods for using such an organism to produce adipate, 6-aminocaproate, hexamethylenediamine or caprolactam.Type: GrantFiled: December 16, 2013Date of Patent: December 11, 2018Assignee: Genomatica, Inc.Inventors: Anthony P. Burgard, Robin E. Osterhout, Stephen J. Van Dien, Cara Ann Tracewell, Priti Pharkya, Stefan Andrae
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Patent number: 10150977Abstract: The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.Type: GrantFiled: September 12, 2016Date of Patent: December 11, 2018Assignee: Genomatica, Inc.Inventors: Mark J. Burk, Anthony P. Burgard, Robin E. Osterhout, Priti Pharkya
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Patent number: 10150978Abstract: The present invention relates to a method of producing cellulosic sugar from biomass by effective impurity removal and sugar separation, and more particularly to a method for producing cellulosic sugar from biomass. The method includes pretreating the biomass so as to be separated into a solid and a liquid; subjecting the separated liquid to monomerization, concentration and adsorption processes, thereby obtaining xylose; and subjecting the separated solid to second pretreatment, saccharification and lignin separation processes, thereby obtaining glucose. The method allows fermentation inhibitory substances to be removed from pretreated biomass in a cost- and energy-effective process, and is useful for effective production of cellulosic sugar.Type: GrantFiled: November 3, 2016Date of Patent: December 11, 2018Assignee: SK Innovation Co., Ltd.Inventor: Min Su Koo
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Patent number: 10150979Abstract: The invention relates to bacterial mutants, particularly from Streptococcus agalactiae, that secrete capsular polysaccharide and methods of purifying the secreted bacterial capsular polysaccharides from culture medium. The extracted polysaccharides are useful for producing vaccines comprising the polysaccharides alone or conjugated to proteins.Type: GrantFiled: May 5, 2015Date of Patent: December 11, 2018Assignee: GlaxoSmithKline Biologicals SAInventors: Evita Balducci, Francesco Berti, Valdemar Robert Janulczyk, Immaculada Margarit Y Ros, Chiara Toniolo
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Patent number: 10150980Abstract: The invention concerns a process for deactivating a cellulolytic microorganism enabling the production of an enzymatic cocktail, said cocktail being used without separating the cellulolytic microorganism during the biochemical conversion of lignocellulosic materials, comprising at least one step for bringing a gaseous stream into contact with a medium containing said microorganism, said gaseous stream comprising more than 25% by weight of CO2 and comprising less than 0.5 molar % of O2.Type: GrantFiled: June 5, 2013Date of Patent: December 11, 2018Assignees: L'Institut National de la Recherche Agronomique, Agro Industrie Recherches et DeveloppementsInventors: Fadhel Ben Chaabane, Sylvain Louret
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Patent number: 10150981Abstract: A reagent composition for a biosensor sensor strip is disclosed that provides for rapid rehydration after drying. The composition includes porous particles and is preferably formed as a colloidal suspension. The dried reagent composition including porous particles may provide analytically useful output from the sensor strip in a shorter time period than observed from dried reagent compositions using solid particles. The output signal from the porous particle compositions may be correlated to the analyte concentration of a sample within about two seconds. In this manner, an accurate concentration determination of an analyte concentration in a sample may be obtained in less time than from sensor strips including conventional compositions. The reagent composition including the porous particles also may allow for the redox reaction between the reagents and the analyte to reach a maximum kinetic performance in a shorter time period than observed from conventional sensor strips.Type: GrantFiled: June 1, 2016Date of Patent: December 11, 2018Assignee: Ascensia Diabetes Care Holdings AGInventor: Boru Zhu
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Patent number: 10150982Abstract: The disclosure provides assay methods for characterizing the effects of an agent on a microbiome of a subject. Moreover, the disclosure provides methods for practical applications of assay results. The biological sample is extracted and the microbial population is enumerated by using signals or markers specific to the microbial species. The enumerated population is subjected to the action of one or many therapeutic agents and the efficiency is assessed by deriving a score based on the effects in the individual samples and in the population of samples.Type: GrantFiled: March 10, 2014Date of Patent: December 11, 2018Assignee: Second Genome, Inc.Inventors: Rachel Steger, Peter DiStefano, Nadir Mahmood
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Patent number: 10150983Abstract: It is an object of this invention to provide a simple measurement method capable of detecting indoxyl sulfuric acid in a sample rapidly and at high sensitivity. By causing sulfatase and tetrazolium salt to act on indoxyl sulfuric acid in a sample to generate a formazan dye, and then calculating the generation amount of the formazan dye, indoxyl sulfuric acid in the sample can be measured more simply, more rapidly, and at higher sensitivity as compared with former methods.Type: GrantFiled: February 18, 2014Date of Patent: December 11, 2018Assignee: Nipro CorporationInventors: Kenta Tatsumi, Daisuke Nakamura
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Patent number: 10150984Abstract: Disclosed are compositions and methods for measuring tyrosine kinase activity.Type: GrantFiled: November 22, 2016Date of Patent: December 11, 2018Inventors: Laurie L Parker, Andrew Michael Lipchik, Scott Charles Bolton
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Patent number: 10150985Abstract: Provided are methods of depleting a target nucleic acid from an initial collection of nucleic acids. Aspects of the methods include contacting the initial collection with a nucleic acid guided nuclease specific for the target nucleic acid in a manner sufficient to deplete the target nucleic acid from the initial collection. Depending on a given application, depletion of a target nucleic acid may vary, e.g., where depleting may include cleaving a target nucleic acid in, or selectively separating a target nucleic acid from, the initial collection of nucleic acids. Also provided are compositions and kits for practicing embodiments of the methods.Type: GrantFiled: December 23, 2014Date of Patent: December 11, 2018Assignee: Takara Bio USA, Inc.Inventors: Andrew Alan Farmer, Craig Betts, Nathalie Bolduc
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Patent number: 10150986Abstract: The present application relates to biosensors, for example comprising an high-molecular weight, tandem repeating, structure-switching nucleic acid aptamers (concatemeric aptamers) to rapidly create patterned sensors via, for example, inkjet printing. These concatemeric aptamer reporters remain immobilized at the point of printing through strong adsorption but retain sufficient segmental mobility to undergo structure switching and reporter signalling to provide both qualitative and quantitative detection of one or more analytes. In certain embodiments, inkjet printing allows for the patterning of internally referenced sensors with multiplexed detection, and provides a generic platform for on-demand printing of sensors even in remote locations.Type: GrantFiled: March 24, 2016Date of Patent: December 11, 2018Assignee: MCMASTER UNIVERSITYInventors: John D. Brennan, Yingfu Li, Carmen Carrasquilla
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Patent number: 10150987Abstract: The present invention provides labeled circular plasmid DNA molecules for studying DNA topology and topoisomerases. The molecules of the present invention also provide tools for high throughput drug screening for inhibitors of DNA gyrases and DNA topoisomerases for anticancer drug discovery and antibiotics discovery.Type: GrantFiled: December 28, 2017Date of Patent: December 11, 2018Assignee: THE FLORIDA INTERNATIONAL UNIVERSITY BOARD OF TRUSTEESInventor: Fenfei Leng
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Patent number: 10150988Abstract: The present invention generally relates to methods for detecting a target in a sample; methods for modulating the reporting intensity of a labeled target in a sample of fixed cells or tissues; methods for detecting the location of at least two targets in a sample; and related compositions.Type: GrantFiled: August 13, 2013Date of Patent: December 11, 2018Assignee: WILLIAM MARSH RICE UNIVERSITYInventors: Michael Diehl, Jan Zimak, Ryan Schweller, Edward B. Samson, Dzifa Y. Duose
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Patent number: 10150989Abstract: The present invention discloses the identification of a fibrosis susceptibility gene locus, the IL22RA2 gene locus, which can be used for detecting predisposition to, diagnosis and prognosis of fibrosis as well as for the screening of therapeutically active drugs. The invention further provides a method for determining the likelihood of a patient affected with a viral infection to respond to a treatment with an antiviral agent and/or an interferon, which method comprises determining alteration in IL22RA2 gene locus or in IL22RA2 expression or IL22RA2 protein activity in a biological sample of the patient.Type: GrantFiled: August 3, 2012Date of Patent: December 11, 2018Assignees: UNIVERSITE D'AIX MARSEILLE, INSERMInventors: Alain Dessein, Mathieu Sertorio, Laurent Argiro
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Patent number: 10150990Abstract: The present application provides polynucleotides comprising 5?-tails with sequence segments useful for the detection of target nucleic acid sequences, and methods for their use in detecting target nucleic acids. The polynucleotides are used to amplify a subsequence of a target nucleic acid in the presence of one or more ribonucleotides. The ribonucleotides are incorporated into amplification products at regular intervals complementary to the 5?-tail sequence segments. Cleavage of amplification products at the bond immediately 3? to incorporated ribonucleotides produces detectably distinct fragments indicative of the presence or absence of a target nucleic acid.Type: GrantFiled: April 9, 2009Date of Patent: December 11, 2018Assignees: Roche Molecular Systems, Inc., CEA/Institut de Genomique—Centre National de GenotypageInventors: David H. Gelfand, Ivo Glynne Gut, Keith A. Bauer, Florence Mauger
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Patent number: 10150991Abstract: Improvements on the basic method used for BEAMing increase sensitivity and increase the signal-to-noise ratio. The improvements have permitted the determination of intrinsic error rates of various DNA polymerases and have permitted the detection of rare and subtle mutations in DNA isolated from plasma of cancer patients.Type: GrantFiled: June 6, 2016Date of Patent: December 11, 2018Assignee: The Johns Hopkins UniversityInventors: Bert Vogelstein, Frank Diehl, Kenneth W. Kinzler, Meng Li
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Patent number: 10150992Abstract: A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.Type: GrantFiled: July 5, 2016Date of Patent: December 11, 2018Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Steven M. Menchen, Guobing Xiang, Prasanna Krishnan Thwar, Jeffrey Frediani, Allen Wong, Alfred Lui, Lily Lu
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Patent number: 10150993Abstract: Provided herein is technology relating to depositing and/or placing a macromolecule at a desired site for an assay and particularly, but not exclusively, to methods and systems for placing or guiding a macromolecule such as a protein, a nucleic acid, or a protein: nucleic acid complex to an assay site, such as near a nanopore, a nanowell, or a zero mode waveguide.Type: GrantFiled: December 21, 2012Date of Patent: December 11, 2018Assignee: IBIS BIOSCIENCES, INC.Inventor: Mark A. Hayden
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Patent number: 10150994Abstract: Modular flow cells, devices with modular flow cells, and methods of sequencing using modular flow cells, as well as systems and kits including modular flow cells, are described, permitting sequencing wherein less than the full capacity for sequencing is desired.Type: GrantFiled: July 20, 2016Date of Patent: December 11, 2018Assignees: Qiagen Waltham, Inc., Qiagen Instruments AGInventors: Jerzy Olejnik, Dirk Zimmermann
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Patent number: 10150995Abstract: Provided herein is a method for analyzing polynucleotides such as genomic DNA. In certain embodiments, the method comprises: (a) treating chromatin isolated from a population of cells with an insertional enzyme complex to produce tagged fragments of genomic DNA; (b) sequencing a portion of the tagged fragments to produce a plurality of sequence reads; and (c) making an epigenetic map of a region of the genome of the cells by mapping information obtained from the sequence reads to the region. A kit for performing the method is also provided.Type: GrantFiled: July 24, 2018Date of Patent: December 11, 2018Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Paul Giresi, Jason D. Buenrostro, Howard Y. Chang, William J. Greenleaf
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Patent number: 10150996Abstract: A relative representation of adaptive immune cells in a biological sample is quantified using multiplex PCR and sequencing of adaptive immune cells, control genes, and synthetic template molecules. Disclosed herein are methods for quantifying a number of adaptive immune cells in a biological sample, and methods for quantifying a relative representation of adaptive immune cells in a biological sample that comprises a mixture of cells comprising adaptive immune cells and cells that are not adaptive immune cells. Methods are provided for amplifying by multiplex PCR and sequencing a first set of synthetic templates each comprising one TCR or IgV segment and one TCR of Ig J or C segment and a unique bar code.Type: GrantFiled: March 5, 2015Date of Patent: December 11, 2018Assignee: ADAPTIVE BIOTECHNOLOGIES CORP.Inventors: Harlan Saul Robins, Christopher Scott Carlson, Mark J. Rieder, Anna M. Sherwood, Ryan O. Emerson