Abstract: The invention provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides non-naturally occurring or engineered DNA-targeting systems comprising a novel DNA-targeting CRISPR effector protein and at least one targeting nucleic acid component like a guide RNA. Methods for making and using and uses of such systems, methods, and compositions and products from such methods and uses are also disclosed and claimed.

Abstract: A system for characterizing intercellular communication and heterogeneity in cancer tumors, and more particularly a method for detecting sub-populations and receptor-ligand states for providing predictive information in relation to cancer and cancer treatment is disclosed. The system comprises the steps of obtaining from a NGS sequencer, single-cell RNA-seq for a plurality of cells within a tumor, correlation with a plurality of data sets from a curated gene list of receptor-ligand pairs, normalizing their transcript abundance data, assigning states (e.g. 0,1,2,3) to each curated receptor-ligand pair in each cell (e.g.

Abstract: The present invention provides for methods for discriminating between alleles at polymorphic positions in a genome. In general the methods employ allele specific extension of oligonucleotides that are complementary to one of the alleles at the 3? end of the oligonucleotide. The allele specific oligonucleotides are resistant to proof reading activity from a polymerase and may be extended in an allele specific manner by a DNA polymerase with a functional 3? to 5? exonuclease activity. The allele specific oligonucleotides may be attached to a solid support such as a chip or a bead.

Abstract: The present disclosure provides devices, systems and methods for sequencing nucleic acid molecules. A sequence of a nucleic acid molecule may be identified at high accuracy using a chip comprising an array of sensors, wherein each individual sensor comprises at least one nano-gap electrode pair configured to generate electrical signals upon flow of the nucleic acid molecule through or in proximity to at least one nano-gap of the nano-gap electrode pair.

Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterisation using CsgG.

Abstract: Modular flow cells, devices with modular flow cells, and methods of sequencing using modular flow cells, as well as systems and kits including modular flow cells, are described, permitting sequencing wherein less than the full capacity for sequencing is desired.

Abstract: A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.

Abstract: This invention relates to a method of determining the susceptibility of an individual to statin-induced myopathy, comprising detecting the presence or absence of one or more polymorphisms in the SLCO1B1 gene in a biological sample from an individual, whereby the presence of one or more polymorphisms indicates that the individual has altered susceptibility to statin-induced myopathy.

Abstract: Technologies for assessing, quantifying and isolating sperm cell populations and/or subpopulations having specific genetic signatures are provided, as well as methods and systems to assess the efficacy of chromosomal differentiation processes. Compositions for identification and differentiation of X-chromosomes and Y-chromosomes in DNA are also provided.

Abstract: The compositions and methods provided herein allow for identification of causative genetic biomarkers for a disease condition or drug response.

Abstract: Systems, methods, and apparatuses are provided for diagnosing auto-immune diseases such as systemic lupus erythematosus (SLE) based on the sizes, methylation levels, and/or genomic characteristics of circulating DNA molecules. Patients provide blood or other tissue samples containing cell-free nucleic molecules for analysis. Massively parallel and/or methylation-aware sequencing can be used to determine the sizes and methylation levels of individual DNA molecules and identify the number of molecules originating from different genomic regions.

Abstract: The present invention provides a method of diagnosing the existence or risk of hyperthyroidism in a feline comprising measuring the level of expression of one or more biomarkers selected from the group consisting of, e.g., IYD, TG, SLC5A5, NIS, TPO, TSHR, DUOX1, DUOX2 (ThOX), TGFB1, CSTD, DCN and SEPP1 and the expression products thereof, in a biological sample from the feline, wherein elevated expression of the one or more biomarkers in the sample relative to a control value for expression in a sample from a normal feline or feline population, or a baseline value from the feline, indicates the existence or risk of hyperthyroidism; a method of treating a feline so diagnosed; and compositions, reagents and kits for carrying out the specified methods.

Abstract: Method and kits for diagnosing propensity to non-contact cranial cruciate ligament rupture (CCLR) in a dog are described. The method includes isolating genomic DNA from a dog and then analyzing the genomic DNA from step for a single nucleotide polymorphism occurring in selected loci that have been determined to be associated with the CCLR phenotype via a genome-wide association study.

Abstract: Provided herein is a method for enriching a sample for polynucleotides comprising a breakpoint of a fusion gene, comprising: a) contacting a probe set comprising a plurality of polynucleotide probes, each probe configured to specifically hybridize to a fusion gene, wherein the set comprises one or more high affinity polynucleotide probes (e.g., a polynucleotide comprising one or more locked nucleic acid nucleotides), with a mixture of polynucleotides under hybridization conditions to produce probe-captured polynucleotides; and b) isolating the probe-captured polynucleotides from the mixture, to produce a sample enriched with polynucleotides comprising breakpoint fragments of the fusion gene.

Abstract: In one aspect, the invention features a method for identifying a drug-modulated polypeptide substrate of cereblon (CRBN). In another aspect, the invention features a method of identifying a polypeptide target of a modulator of CRBN. In yet another aspect, the invention provides methods of monitoring or characterizing the sensitivity of a subject to a modulator of CRBN.

Abstract: Disclosed are systems and methods for detecting genetic alterations comprising androgen receptor gene splice variants (AR-Vs), mutations, indel, copy number changes, fusion and combination thereof, in a biofluid sample from the patient. The systems and methods are similarly applicable to the detection of gene alterations comprising gene splicing variants, mutations, indel, copy number changes, fusion and combination thereof of other genes of interest. The streamlined methods improve the consistency and simplicity of non-invasive detections of biomarkers.

Abstract: The present invention relates to methods and compositions for determining whether a subject having prostate cancer is at greater risk of developing progressive disease, and methods of treating the subjects. It is based, at least in part, on the discovery that approximately 90% of men carrying at least one of the following fusion genes: TRMT11-GRIK2, SLC45A2-AMACR, MTOR-TP53BP1, LRRC59-FLJ60017, TMEM135-CCDC67 and CCNH-05orf30 experienced prostate cancer recurrence, metastases and/or prostate cancer-specific death after radical prostatectomy (each examples of “progressive prostate cancer”), while these outcomes occurred in only 36% of men not carrying any of these fusion genes. It is also based, at least in part, on the discovery that no patient studied survived five years without recurrence if their primary prostate cancer contained a TRMT11-GRIK2 or MTOR-TP53BP1 fusion gene. It is also based, at least in part, on the discovery that the protein encoded by the MAN2A1-FER fusion gene exhibits kinase activity.

Abstract: A method for detecting genes sensitive to low-level ionizing radiation and genes detected by the method. More specifically, genes sensitive to low-level ionizing radiation and related to suppressing thymic cancer, discovered in a carcinogenic entity and verified in a normal entity are detected by subjecting a cancerous AKR/J mouse and a normal ICR mouse to low-level radiation. Thymus is collected therefrom, immunogenic and apoptotic genes are classified via microarray processing of the thymus. The genes are amplified and the levels of gene expression are measured. Thus, a gene having a specific reaction to radiation can be accurately detected by preventing the interference of confounding variables.

Abstract: The present invention provides a new target analysis method and a target analysis kit for use in the method. The target analysis method of the present invention includes: causing a specimen, a labeled binding nucleic acid molecule that binds to a target, and a carrier on which a blocking nucleic acid molecule that binds to the labeled binding nucleic acid molecule is immobilized to react; separating a fraction of the carrier and a fraction of components other than the carrier from each other; and detecting a label of the labeled binding nucleic acid molecule in at least one of the fraction of the carrier and the fraction of components other than the carrier to analyze a target in the specimen.

Abstract: Provided is a method for continuously visualizing and accurately determining a living cellular state per se. Disclosed are: a vector comprising 5?- and 3?-end nucleic acid sequences for integration and a reporter gene sequence positioned between the 5?- and 3?-end nucleic acid sequences for integration; a gene introduction agent comprising a first vector and a second vector; a cell comprising the vector or the agent; and a method for determining a cellular state by using the cell.

Abstract: Provided herein is a RNA-based riboregulator that detects protein translation and uses this stimulus to regulate expression of an unrelated protein. In one embodiment, provided herein is a synthetic nucleic acid molecule configured as a translation-sensing riboregulator, where the synthetic nucleic acid molecule comprises a first nucleotide sequence encoding a polypeptide, a translation-sensing riboswitch (TSR), and a second nucleotide sequence encoding a polypeptide, wherein the translation-sensing riboswitch comprises a stop codon of the first nucleotide sequence, a fully or partially double-stranded stem-forming domain, and translation initiation elements of the second nucleotide sequence.

Abstract: The field of the invention encompasses synthetic chromosome compositions and methods that allow single cell spatiotemporal analysis in response to differentiation cues and labeling of transplanted cells to monitor the fate and function of such cells in the patient recipient.

Abstract: The present invention relates to a agave syrup product having a low water content. The agave syrup product retains the physical and palatable properties of untreated agave syrup while having a prolonged shelf-life. It can be advantageously used to sweeten beverages (such as hot beverages) and in the manufacture of pharmaceutical compositions (such as throat lozenges) and/or confectionary.

Abstract: A method ensuring excellent chemical resistance to a chemical cleaning solution, excellent filtration performance and an enhanced life in a saccharified solution production method including a cleaning step and filtration using a porous filtration membrane.

Abstract: A liquid slag granulating system includes a granulator (1), a granulator connecting part and a granulator drive part. The granulator (1) is disc-shaped or cup-shaped, and a diversion cone is arranged at a center of the granulator (1). The granulator drive part drives the granulator to rotate, thus granulating liquid slag. The system further includes an air supply part.

Abstract: The present invention concerns a granulation installation for granulating molten material produced in a metallurgical plant, the installation includes a water injection device, for injecting granulation water into a flow of molten material and thereby granulating the molten material; a granulation tank for collecting the granulation water and the granulated material; and a steam condensation system including a steam collecting hood located above the granulation tank, for collecting steam generated in the granulation tank, a gas conduit arranged between the steam collecting hood and a water column, and a gas compressor arranged within said gas conduit for compressing the steam before feeding it into the water column.

Abstract: This invention provides a manufacturing method of a multi-component alloying steel with high strength and high wear-resistance and a hot rolled plate thereof. The weight percentages of the chemical composition of the wear-resistant steel above is as follows: C: 1.0-1.2%, Si: 0.6-1.0%, Mn: 8.0-10.0%, P<0.02%, S<0.02%, Cr: 1.5-2.5%, Mo: 0.2-0.5%, V: 0.6-1.0%, Nb: 0.02-0.06%, Ti: 0.01-0.1%, Al: 0.03-0.08%, and the rest are Fe and unavoidable impurities. The manufacturing process of the hot rolled plate of the wear-resistant steel includes the steel making, continuous casting, heating, hot rolling and heat treatment.

Abstract: A steel sheet, a plated steel sheet, and methods for producing a hot-rolled steel sheet, a cold-rolled full hard steel sheet, and a steel sheet. The steel sheet has a specified composition and a microstructure including 0 to 60% of polygonal ferrite and 40 to 100% of a total of martensite, bainite, and residual austenite in terms of an area ratio within 20 ?m from the steel sheet surface. The content of Mn in martensite present within 20 ?m of the steel sheet surface ([Mn]SM) and the content of Mn in a bulk ([Mn]B) satisfy [Mn]SM/[Mn]B?1.5. At a location 300 ?m from the steel sheet surface, an area ratio of the martensite is in a range of 40 to 80%, and the polygonal ferrite and the bainite have an average crystal grain size of less than 15 ?m.

Abstract: Provided is a high-strength steel sheet for use in lightening materials for electronic products and materials for vehicles including automobiles, trains and ships and, more particularly, to a high-strength cold rolled steel sheet, a coated steel plate, and a method for manufacturing the same, wherein yield strength and ductility are improved by controlling the internal oxidation depth and the amount of residual austenite after first annealing operation, and the high-strength cold rolled steel sheet and the coated steel plate is stably manufactured and provided without any dent defects during the manufacturing thereof.

Abstract: Provided are a cold-rolled steel sheet having excellent bake hardenability and aging resistance, and manufacturing method thereof. The cold-rolled steel sheet comprises, by weight, 0.02 to 0.08% of carbon (C), 1.3 to 2.1% of manganese (Mn), 0.3% or less (excluding 0%) of silicon (Si), 1.0% or less (excluding 0%) of chromium (Cr), 0.1% or less (excluding 0%) of phosphorus (P), 0.01% or less (excluding 0%) of sulfur (S), 0.01% or less (excluding 0%) of nitrogen (N), and 0.01 to 0.06% of acid soluble aluminum (sol.Al), comprises one or more selected from the group consisting of 0.2% or less (excluding 0%) of molybdenum (Mo) and 0.003% or less (excluding 0%) of boron (B), and comprises a remainder of iron (Fe) and unavoidable impurities, and comprises, by area, 90 to 99% of ferrite and 1 to 10% of martensite as a microstructure.

Abstract: A method of heat treating a steel workpiece may include two heat treatment stages. In the first stage, the steel workpiece may be heated to a first temperature greater than or equal to its A1 temperature, but less than its A3 temperature to transform the microstructure of the steel workpiece into a multiphase microstructure including grains of ferrite and grains of austenite having an average grain diameter. In the second stage, the steel workpiece may be heated to a second temperature greater than the first temperature to increase the average grain diameter of the grains of austenite. Thereafter, the steel workpiece may be cooled to ambient temperature at a rate sufficient to retain a major portion of the grains of austenite obtained during the first and second heat treatment stages. The as-heat treated steel workpiece may comprise a retained austenite phase dispersed within a ferrite matrix phase at ambient temperature.

Abstract: A method for temperature-treating a manganese steel intermediate product whose alloy includes: a manganese content, which lies in the following manganese range: 3 wt. %?Mn?12 wt. %, a fraction of one or more alloying elements of the group: silicon (Si), aluminum (Al), nickel (Ni), chromium (Cr), molybdenum (Mo), phosphorus (P), sulfur (S), nitrogen (N), copper (Cu), boron (B), cobalt (Co), tungsten (W), an optional carbon content (C) of less than 1 wt. %, an optional content of one or more microalloying elements, wherein the total content of the micro-alloying elements is less than 0.45 wt. %; and as a remainder an iron content (Fe) and unavoidable impurities.

Abstract: A component manufacturing method includes: disposing, in a fluid, an unprocessed component having a hole that has an opening in an outer surface of the unprocessed component; creating a flow of the fluid such that air bubbles resulting from laser peening performed by irradiating an inner wall of the hole of the unprocessed component with a laser beam in the fluid flow along the hole; setting an irradiation area of the laser beam in an inner surface of the hole; and in the fluid of which the flow has been created, irradiating the irradiation area with the laser beam from the side of the outer surface through the opening.

Abstract: The invention relates to a method for rolling a metal product (1), wherein the metal product is subjected to a rolling operation at a first station (2), which rolling operation is controlled by a control device (3), wherein the product (1) is subject to a measurement at a second station (4), wherein the product (1) is subjected to a further processing operation at a third station (6), and wherein the product (1) is in a specified quality at a fourth station (7).

Abstract: A method and to an arrangement for recovery of precious metal. The method includes calculating a first electrical property of a slurry downstream of a retention screen in a first flow direction A either (i) based on a first difference between the first supplied current and the first received voltage, or (ii) based on a first difference between the first supplied voltage and the first received current, calculating a second electrical property of the slurry upstream of the retention screen either (i) based on a second difference between the second supplied current and the second received voltage, or (ii) based on a second difference between the second supplied voltage and the second received current, and calculating the adsorption particle content of the slurry upstream of the retention screen based on a difference between the first and the second electrical property.

Abstract: Disclosed is a method of forming a treated material. The method includes providing a high-speed blender; adding a solvent and brass granules to the blender and blending at high speed until mixed; adding copper granules to the blender and mixing at high speed until mixed; adding carbon nanotubes and graphene to the blender and mixing until blended. The mixture of solvent, brass granules, copper granules, carbon granules, carbon nanotubes, and graphene are added to an additional mixture of brass and copper and mixed until all of the granules are uniformly saturated. The mixture is then dried to a powder. Thereafter, the dry powder may be added to ferrous or nonferrous metal(s) in a high temperature crucible and then heated until melted.

Abstract: The invention concerns a process to manufacture a flat-rolled product, notably for the aeronautic industry containing aluminum alloy comprising 2.1% to 3.9% Cu by weight, 0.7% to 2.0% Li by weight, 0.1% to 1,0% Mg by weight, 0% to 0.6% Ag by weight, 0% to 1% Zn by weight, at least 0.20% Fe +Si by weight, at least one element chosen from Zr, Mn, Cr, Sc, Hf and Ti, the quantity of said element, if chosen, being 0.05% to 0.18% by weight for Zn, 0.1% to 0.6% by weight for Mn, 0.05% to 0.3% by weight for Cr, 0.02% to 0.2% by weight for Sc, 0.05% to 0.5% by weight for Hf and 0.01% to 0.15% by weight for Ti, the other elements at most 0.05% by weight each and 0.15% by weight in total, the rest being aluminum, in which, notably a flattening and/or stretching is performed with a cumulated deformation of at least 0.5% and less than 3%, and a short heat-treatment is performed in which the sheet reaches a temperature between 130° C. and 170° C. for a period of 0.1 to 13 hours.

Abstract: An object of the invention is to provide a two-phase alloy which contains inexpensive Cr as a main component, and which is superior in strength properties including corrosion resistance and toughness, and in abrasion resistance to conventional ones under a high corrosion circumstance such as in an oil well. The invention is a Cr based two-phase alloy including two phases of a ferrite phase and an austenite phase in a mixed state, in which a chemical composition of the Cr based two-phase alloy consists of a major component, an accessory component, impurities, a first optional accessory component, and a second optional accessory component, and the major component consists of 33% by mass or more and 65% by mass or less of Cr, 18% by mass or more and 40% by mass or less of Ni, and 10% by mass or more and 33% by mass or less of Fe.

Abstract: The present invention relates to a high-entropy alloy especially having excellent low-temperature tensile strength and elongation by means of having configured, through thermodynamic calculations, an alloy composition region having an FCC single-phase microstructure at 700° C. or higher, and enabling the FCC single-phase microstructure at room temperature and at an ultra-low temperature. The high-entropy alloy, according to the present invention, comprises: Co: 3-12 at %; Cr: 3-18 at %; Fe: 3-50 at %; Mn: 3-20 at %; Ni: 17-45 at %; V: 3-12 at %; and unavoidable impurities, wherein the ratio of the V content to the Ni content (V/Ni) is 0.5 or less, and the sum of the V content and the Co content is 22 at % or less.

Abstract: A spheroidal graphite cast iron meeting N(5-)?250, N(5-20)/N(5-)?0.6, and N(30-)/N(5-)?0.2, wherein N(5-) represents the number (/mm2) of graphite particles having equivalent-circle diameters of 5 ?m or more, N(5-20) represents the number (/mm2) of graphite particles having equivalent-circle diameters of 5 ?m or more and less than 20 ?m, and N(30-) represents the number (/mm2) of graphite particles having equivalent-circle diameters of 30 ?m or more, among graphite particles observed in an arbitrary cross section of at least 1 mm2.

Abstract: A high-strength steel sheet comprising, as component composition, by mass: C: 0.15 to 0.35%; total of Si and Al: 0.5 to 2.5%; Mn: 1.0 to 4.0%; P: more than 0% and 0.05% or less; and S: more than 0% and 0.01% or less, with the balance being Fe and inevitable impurities, wherein a steel structure satisfies, in ratio with respect to the whole structure: ferrite: more than 5 area % and 50 area % or less; total of tempered martensite and bainite: 30 area % or more; and retained austenite: 10 volume % or more, the steel structure further includes MA, and the steel structure satisfies: an average circle equivalent diameter of the MA: 1.0 ?m or less; an average circle equivalent diameter of the retained austenite: 1.0 ?m or less; and a volume ratio of retained austenite with a circle equivalent diameter of 1.5 ?m or more to the whole retained austenite: 5% or more.

Abstract: There are provided a very thin martensitic stainless steel foil and a manufacturing method thereof, which are capable of reducing shape defects and the like. A martensitic stainless steel foil of the present invention has a thickness of at most 35 ?m, and having a steepness of at most 0.75% when the steel foil has a length of 650 mm. Preferably, a metallographic structure in a cross-section of the steel foil is a ferrite structure, in which carbides are dispersed. More preferably, the steel foil consisting of, by mass, 0.25% to 1.5% C, 10% to 18% Cr, at most 1.0% Si (exclusive of 0%), at most 1.5% Mn (exclusive of 0%), at most 3.0% Mo (inclusive of 0%), and the balance of Fe with inevitable impurities.

Abstract: A strain-hardenable stainless steel alloy includes hard secondary phases dispersed in an austenitic primary phase, the alloy including 0.3-0.6% nitrogen by weight.

Abstract: A production method for molten-aluminum-plated steel wire, the production method being characterized in that a steel wire (2) immersion part (6) at which the steel wire (2) is to be immersed in a molten aluminum plating bath (1) is immersed in the molten aluminum plating bath (1) after the steel wire (2) has been passed into a steel wire introduction device (7) while an immersion region (9a) thereof has been immersed in the molten aluminum plating bath (1). The steel wire introduction device (7) comprises a tube-shaped body (9), which has a total length of 10 to 1000 mm and has a through hole (8) that is for passing the steel wire (2) through the inside thereof, and includes the immersion region (9a), which is for immersion in the molten aluminum plating bath (1) to a length of 2 mm to 400 mm from an end part of one end of the tube-shaped body (9) along the long direction of the tube-shaped body (9).

Abstract: Provided are a system and process for remanufacturing a waste cylinder assembly of an aircraft piston engine. The spraying apparatus includes a first power mechanism, a spray gun assembly and a second power mechanism. The first power mechanism drives the cylinder assembly to move in a horizontal direction and a vertical direction. The second power mechanism drives the spray gun assembly to rotate around a center of the blind hole and ensures that prepared coatings can be evenly distributed along an inner wall of the blind hole. A nozzle end of the spray gun extends into the blind hole, and the spray gun is adjustable relative to the center of the blind hole. A spraying distance is not fixed so as to change the spraying distance. Powder can be fully melted.

Abstract: Provided is a scan vapor deposition metal mask with which a high-resolution, linear vapor deposition film pattern can be formed even on a large-sized substrate. Two first unit opening groups are included. The first unit opening groups are arranged by being shifted from each other in a Y direction so as not to be adjacent to each other in an X direction, while the first unit opening groups adjacent to each other in the Y direction are arranged by being shifted from each other in the X direction by ½ times a first opening pitch.

Abstract: A mask carrier and an evaporation system are provided. The mask carrier includes a base and at least two correction fixtures. The at least two correction fixtures are configured to clamp, on the base, a framework of a metal mask which is used for evaporation on the base, the base has a first opening through which organic material passes, and the at least two correction fixtures are around the first opening in a spaced manner. During organic evaporation coating, the at least two correction fixtures on the mask carrier press the framework of the metal mask to prevent the framework from deformation. Thus, a clearance between a substrate and the metal mask is reduced or even eliminated after the substrate is in press-fit with surface of the metal mask. Patterns obtained through evaporation are clear in shape and outline and normal in size. Hence, the product yield is increased.

Abstract: A cover window for a display device and a method of manufacturing the same are provided. The cover window for a display device includes a light-transmitting substrate, a mold layer on at least a part of an edge of the light-transmitting substrate, and having an uneven pattern formed on a surface thereof, an inorganic material pattern layer on the mold layer and exposing a portion of the mold layer, and a print layer on the mold layer and covering the exposed portion of the mold layer.

Abstract: The invention provides a method for producing a PVD anti-bacterial film on plastic. By adoption of the technical scheme, film plating can be achieved on the premise of not damaging a plastic workpiece without heating during the whole process, and a titanium target and a silicon target are used for plating a base film; plastic mainly contains a large quantity of carbon atoms and silicon atoms and has good bonding force, so that the bonding force of the film and the workpiece can be increased by plating the base film through the titanium target and the silicon target; a silver target is added for sputtering after the base film is plated, so that silver with an anti-bacterial effect is evenly distributed in a titanium silicide film, and the anti-bacterial film with the anti-bacterial effect is formed.

Abstract: This invention relates to a gold coated copper layer, comprising: a metal layer formed of a copper-containing material; a metal protective layer positioned on the metal layer and formed of brass, manganese brass, phosphor bronze, delta metal, naval brass, aluminum-brass alloy, copper-tin alloy, bronze, or copper-lead alloy; and a gold layer on the metal protective layer.