Abstract: This invention features systems and methods for the detection of analytes, and their use in the treatment and diagnosis of disease.

Abstract: A method of predicting cognitive performance for a person, by acquiring a genetic sample from the person, determining an SNP of the rs914246 of the FTCD gene of the person, comparing the determined SNP against a cognitive model, and based on the comparison, returning a predicted cognitive performance.

Abstract: Methods of labelling one or more subcellular components (e.g., an organelle and/or subcellular region) in vivo are provided. Methods of labelling a protein in vivo are provided. Methods of determining a nucleic acid sequence in situ are also provided.

Abstract: The disclosed embodiments concern methods, systems and computer program products for determining sequences of interest using unique molecular indexes (UMIs) that are uniquely associable with individual polynucleotide fragments, including sequences with low allele frequencies or long sequence length. In some implementations, the UMIs include variable-length nonrandom UMIs (vNRUMIs). Methods and systems for making and using sequencing adapters comprising vNRUMIs are also provided.

Abstract: A method of detecting a target molecule including binding, to first target molecules, specific binding substances which are capable of recognizing different epitopes and labeled with nucleic acid fragments, respectively, detecting the specific binding substances based on fluorescence signals of different fluorescence wavelengths from the nucleic acid fragments, and detecting the first target molecules based on the fluorescence signals that correspond to the specific binding substances, respectively.

Abstract: The present disclosure provides biochips and methods for making biochips. A biochip can comprise a nanopore in a membrane (e.g., lipid bilayer) adjacent or in proximity to an electrode. Methods are described for forming the membrane and inserting the nanopore into the membrane. The biochips and methods can be used for nucleic acid (e.g., DNA) sequencing. The present disclosure also describes methods for detecting, sorting, and binning molecules (e.g., proteins) using biochips.

Abstract: Various embodiments of the teachings relate to a system or method for sample preparation or analysis in biochemical or molecular biology procedures. The sample preparation can involve small volume processed in discrete portions or segments or slugs, herein referred to as discrete volumes. A molecular biology procedure can be nucleic acid analysis. Nucleic acid analysis can be an integrated DNA amplification/DNA sequencing procedure.

Abstract: This invention provides novel azido linkers for deoxynucleotide analogues having a detectable marker attached thereto.

Abstract: A technique for sequencing nucleic acids in an automated or semi-automated manner is disclosed. Sample arrays of a multitude of nucleic acid sites are processed in multiple cycles to add nucleotides to the material to be sequenced, detect the nucleotides added to sites, and to de-block the added nucleotides of blocking agents and tags used to identify the last added nucleotide. Multiple parameters of the system are monitored to enable diagnosis and correction of problems as they occur during sequencing of the samples. Quality control routines are run during sequencing to determine quality of samples, and quality of the data collected.

Abstract: A flow cell article including: a chamber; and at least one surface of the chamber comprising: a solid substrate having a reactive surface comprising: a coupling agent covalently attached to the solid substrate; a polymer of the formula (I) as defined herein, covalently attached to the coupling agent; and a nucleic acid probe covalently attached to the polymer. Also disclosed is a method of making the article and a method of using the article.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing. In some cases, this disclosure provides methods for the generation of polynucleotide barcode libraries, and for the attachment of such polynucleotides to target polynucleotides.

Abstract: Aspects of the present invention include analyzing nucleic acids from single cells using methods that include using tagged polynucleotides containing multiplex identifier sequences.

Abstract: Provided herein is a multi-sample and multi-locus method for analyzing a genetic locus. In particular, provided herein is a method for SNP detection and analysis based on high-throughput sequencing, comprising designing a probe, pre-amplification and biotin labeling, hybridization, ligation, barcode specific primer extension, sequencing and analyzing the SNP locus. A probe set is for the analysis is also provided.

Abstract: The present invention provides methods for defining a disease or condition with a wide range of etiologies based on clustering of genes belong to the same biological pathway and observed frequencies or prevalence of various patient factors assessed in a given patient population, including motor and non-motor symptoms, neuropathology, age of onset, genetics, and involvement of peripheral autonomic system, including the enteric nervous system of the gastrointestinal (GI) system. Also disclosed herein are methods and kits for early diagnosis and treatment of subjects for multisystem Lewy body disease (MLBD), including Parkinson's disease, and/or one or more GI conditions that indicate a propensity for MLBD.

Abstract: Provided is a method of diagnosing and/or monitoring traumatic brain injury (TBI) in a subject. The method comprises determining a level of at least one miRNA in a fluid sample from the subject. The miRNA may be selected from mat-425-5p, miR-502, milt-21 and miR-335. The method may involve determining whether a subject is suffering from mild-TBI or moderate-to-severe TBI. Also provided is a sensor element, a detection system, composition and a kit for diagnosing and/or monitoring TBI, and a method of determining an appropriate treatment for a subject with a suspected TBI.

Abstract: Methods, compositions, and systems are provided for detecting one or more a gastrointestinal issues by characterizing the microbiome of an individual, monitoring such effects, and/or determining, displaying, or promoting a therapy for the gastrointestinal issue. Methods, compositions, and systems are also provided for generating and comparing microbiome composition and/or functional diversity datasets. Methods, compositions, and systems are also provided for generating a characterization model and/or therapy model for constipation issues, diarrhea issues, hemorrhoids issues, bloating issues, and lactose intolerance issues.

Abstract: Provided herein are compositions, systems, kits, and methods for detecting rejection, or an elevated risk of rejection, of an organ or tissue graft (e.g., kidney graft) in a subject by detecting one, or a panel of, RNA markers in a urine sample from the subject. In certain embodiments, kidney graft injury is detected by measuring one or more of the RNA markers disclosed herein. In some embodiments, the one or more markers are employed to distinguish between acute graft rejection and BK virus nephropathy.

Abstract: The present invention relates to compositions and methods for detecting cell death by detecting cell DNA in a biological sample. The invention relates the discovery that the presence of hypomethylated ? cell DNA outside of the pancreas of a subject is indicative of ? cell death. Thus, in one embodiment, the invention is a method of detecting hypomethylated ? cell insulin DNA in a biological sample of a subject including the steps of: obtaining a biological sample from the subject, where the biological sample is obtained from outside of the subject's pancreas and where the biological sample contains ? cell insulin DNA; determining the methylation status of at least one of the CpG dinucleotides in the ? cell insulin DNA, where when at least one of the CpG dinucleotides in the ? cell insulin DNA is determined to be unmethylated, the hypomethylated ? cell insulin DNA is detected.

Abstract: The present invention relates to a method of determining the susceptibility of a dog to, or the likelihood that a dog is protected from, hip dysplasia. The present invention uses mutations linked to canine hip dysplasia to generate a model predicting disease.

Abstract: Provided herein are specially modified blocking nucleotides allowing for the sensitive detection of low copies of variant sequences, while significantly reducing signals from non-variant sequences that are similar but not identical to the variant sequence. These nucleotides can be used to detect rare variants in a sample mixture, as described in the present methods.

Abstract: A highly sensitive for determining the expression of PD-L1 that is based on a RT-qPCR in a RNA sample of, for example, Circulating Tumor Cells (CTC) or fresh frozen primary tumor tissues. In particular, to a method for the detection of PD-L1 mRNA positive CTCs or primary tumor tissues (fresh frozen) based on the quantitative determination of the molecular marker (PD-L1) in biological samples of patients suffering from cancer. By using the method, detection can take place before, during or after the immune therapy or any other treatment in order to provide significant information concerning guiding or monitoring of the anti-PD-L1 agents effectiveness. This RT-qPCR assay could comprise a promising companion diagnostic test in order to evaluate the PD-L1 expressional status on CTC or tumor tissue, providing clinical applications, which could have an important impact on therapeutic interventions since the expression of PD-L1 is associated with response to immunotherapy.

Abstract: This invention provides methods of using cell free bodily fluid and blood cells in the diagnosis, prognosis, or monitoring of diseases or conditions. The invention also relates to methods of using cell free bodily fluid and blood cells to identify markers of diseases or conditions.

Abstract: Methods and compositions for treating cancer such as melanomas are disclosed.

Abstract: Provided are methods of identifying whether a subject having cancer will be responsive to agents that combat immune evasion, such as immune checkpoint inhibitors. Methods of treating a subject having cancer are also provided. Such methods may include those that involve identifying whether the cancer will be responsive to an immune checkpoint inhibitor and/or is an immune-evasive cancer and administering an agent, e.g., an immune checkpoint inhibitor and/or a MYC inhibitor, to the subject to treat the cancer. Also provided are methods of identifying cancer therapeutics that are effective during MYC-regulated immune evasion as well as cancer cell lines and transgenic animals useful in such methods. Kits for use in the described methods are also provided.

Abstract: This disclosure describes a kit for determining the level of APOBEC3 in a sample of a subject, and a method for making an anti-APOBEC3 antibody/APOBEC3 complex. This disclosure further describes methods of determining a prognosis of a subject having a cancer using APOBEC3 expression and, in some instances, providing treatment based on the prognosis.

Abstract: Disclosed herein are methods, compositions, and devices for use in early detection of cancer. The methods include sequencing a panel of regions in cell-free nucleic acid molecules and detecting one or more tumor markers that are indicative of a cancer.

Abstract: An isoform-level gene panel is disclosed that can accurately classify a glioblastoma subtype from a tumor sample. Such an isoform level gene panel comprises the 121 to 214 target isoforms identified in Table 1. Also disclosed are reagents for quantitively detecting the expression or activity of the target isoforms of Table 1 in a patient sample. For example, such ligands can be PCR primer and probes sets. This isoform-level gene panel and reagents for detection of the isoforms are useful in an isoform-level assay for diagnosis of the molecular subtype of a glioblastoma in a patient. The assay employs algorithms and a novel computer program that performs the functions of FIG. 8. In one aspect, the assay is a high-throughput format.

Abstract: A method for detecting genes sensitive to low-level ionizing radiation and genes detected by the method. More specifically, genes sensitive to low-level ionizing radiation and related to suppressing thymic cancer, discovered in a carcinogenic entity and verified in a normal entity are detected by subjecting a cancerous AKR/J mouse and a normal ICR mouse to low-level radiation. Thymus is collected therefrom, immunogenic and apoptotic genes are classified via microarray processing of the thymus. The genes are amplified and the levels of gene expression are measured. Thus, a gene having a specific reaction to radiation can be accurately detected by preventing the interference of confounding variables.

Abstract: A method for detecting genes sensitive to low-level ionizing radiation and genes detected by the method. More specifically, genes sensitive to low-level ionizing radiation and related to suppressing thymic cancer, discovered in a carcinogenic entity and verified in a normal entity are detected by subjecting a cancerous AKR/J mouse and a normal ICR mouse to low-level radiation. Thymus is collected therefrom, immunogenic and apoptotic genes are classified via microarray processing of the thymus. The genes are amplified and the levels of gene expression are measured. Thus, a gene having a specific reaction to radiation can be accurately detected by preventing the interference of confounding variables.

Abstract: The presently disclosed subject matter provides methods of diagnosis of cancer or adverse pregnancy outcomes in a subject by measuring amounts of one or more microRNAs present in cancer-derived exosomes isolated from a biological sample from the subject.

Abstract: The technology described herein relates to assays and methods for the diagnosis, prognosis, and/or treatment of melanoma, e.g. relating to measuring the level of neurophilin-2 (NRP-2) mRNA expressed in melanoma cells. In some embodiments, the level of NRP-2 can be normalized to the level of Melan-A (MART) mRNA.

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

Abstract: This invention relates to methods for identifying sunflower plants that having increased Orobanche resistance. The methods use molecular markers to identify and to select plants with increased Orobanche resistance.

Abstract: Provided herein is a dual amplification method for detecting plant pathogens by analysis of pathogen DNA in an unpurified nucleic acid sample from the plant. Pathogen-specific primers are used to generate a first set of amplicons that are further amplified in a second amplification step using fluorescent tagged pathogen-specific primers. Fluorescent amplicons thus generated are hybridized with pathogen-specific nucleic acid probes that are immobilized on a solid support using bifunctional polymer linkers. The hybridized microarray is imaged to obtain fluorescent images of the amplicons and the nucleic acid probes, which are superimposed to detect the pathogen present in the plant. Also described herein is a method to simultaneously detect both plant DNA and pathogen DNA in a single assay.

Abstract: Provided herein is an internal standard method for determining copy number of a pathogen DNA in an unpurified nucleic acid sample by using a known copy number of synthetic DNA that shares a consensus region sequence with the pathogen. The sample is subject to two amplification steps using locus-specific primers and fluorescent primers respectively to obtain fluorescent amplicons for the pathogen and synthetic DNA. These are hybridized with immobilized pathogen-specific and synthetic DNA-specific nucleic acid probes and imaged to obtain fluorescent signals for pathogen-specific and synthetic DNA-specific amplicons. Signal intensities are correlated with the known copy number of synthetic DNA to determine copy number of pathogen DNA in the plant. Also described herein is a method to simultaneously quantitate using the above method, copy numbers of both pathogen and plant DNA in a sample.

Abstract: The present disclosure relates to high-throughput detection of polyketides using genetically encoded biosensors.

Abstract: The present invention provides a method for detecting and/or quantifying the presence of a target nucleic acid sequence of a microorganism in a sample obtained from a subject, including amplifying the target sequence in a CpG island of the nucleic acid of the microorganism, irrespective of the methylation status of the CpG island. The invention is embodies by a method for detecting and/or quantifying the presence of a target nucleic acid sequence of Epstein-Barr virus (EBV) by amplifying a target sequence in the BamHI-W region of EBV in cell free DNAs (cfDNAs) obtained from a subject. The present invention also provides a kit to be used for the method of the invention.

Abstract: The present invention relates to the use of the measure of anelloviral load for the determination of immunosuppression. More precisely, the present invention provides a method for characterizing the immunosuppressed or non-immunosuppressed status of a subject, comprising the steps of determining the anelloviral load from a biological sample of the said subject, and determining from the said comparison the immunosuppressed or non-immunosuppressed status. The determination of the immunosuppressed status of the subject can then be used to design or adapt a therapeutic treatment.

Abstract: The invention relates to an isolated bacterial strain of Pseudomonas aeruginosa species, referred to as Pseudomonas aeruginosa CSMY-1, deposited in the Microbial Genetic Resources Bank of the Chilean Collection of Microbial Genetic Resources (CChRGM), under accession number RGM2262, on Aug. 7, 2015, which is a facultative strain that can remove chemical components having characteristics that pollute natural or industrial effluents or soils by degrading compounds. The invention also relates to a method for the pollutant bioremediation of a contaminated environments, comprising: a) adding bacteria Pseudomonas aeruginosa CSMY-1 in the form of a biofilm to said contaminated environment; and b) incubating said bacteria Pseudomonas aeruginosa CSMY-1 in the form of a biofilm in said environment.

Abstract: Heat treatment of DRI is performed in order to form a DRI product with a metallic shell around at least a portion of the DRI. The heat treatment may be delivered through the use of a plasma torch, a gas burner, an oven, or any other like heat source. The heat treatment may heat the DRI for a fraction of a second and quickly cool the DRI in order to melt the surface and form the metallic shell without vaporizing a significant portion of the DRI and without losing a significant amount of the latent energy in the DRI. During storage and transport of the DRI product, the DRI product is less likely to fracture, the DRI product has less exposed surface area of DRI, and results in reduced DRI fines and/or DRI dust cause by the DRI product rubbing together when compared to traditional types of DRI.

Abstract: Provided herein is a plated steel having high strength with excellent elongation, excellent hole expansibility, and excellent material uniformity, and a method for producing such a plated steel. The steel sheet provided herein has a specific composition, and a steel structure that contains ferrite as a primary phase, and 2 to 12% of perlite, and 3% or less of martensite by volume, and in which the remainder is a low-temperature occurring phase. The ferrite has an average crystal grain diameter of 25 ?m or less. The perlite has an average crystal grain diameter of 5 ?m or less. The martensite has an average crystal grain diameter of 1.5 ?m or less. The perlite has a mean free path of 5.5 ?m or more. The steel sheet has a tensile strength of 440 MPa or more.

Abstract: Disclosed are a wire rod and a manufacturing method therefor. The wire rod comprises in percentage by weight: 0.02 to 0.15% of C; 0.05 to 0.3% of Si; 0.5 to 1.2% of Mn; 0.3 to 0.9% of Cr; 0.02% or less of P; 0.02% or less of S; 0.01 to 0.05% of sol. A1; 0.01% or less of N; Fe as the remainder; and unavoidable impurities, wherein the wire rod satisfies following formulas 1 and 2, wherein, when the hardness of the wire rod measured in 1/2d position and in 1/4d position in the diameter direction of the wire rod is Hv,1/2d(Hv) and Hv,1/4d(Hv), respectively (here, d is the diameter of the wire). [Formula 1] (Hv,1/2d+Hv,1/4d)/2?150 [Formula 2] Hv,1/2d/Hv,1/4d?1.

Abstract: To improve and stabilize magnetic properties, a steel sheet is soaked in a temperature range of 1000° C. or more and 1120° C. or less for 200 sec or less and then soaked in a temperature range of 650° C. or more and 1000° C. or less for 200 sec or less in annealing before final cold rolling, and the amount of Al in precipitates after the annealing before the final cold rolling is limited to 50% or more of the total amount of Al contained in the steel slab.

Abstract: A heat treatment apparatus of one aspect of the present disclosure includes: a feed device that feeds a heat treatment workpiece downstream in a feed direction along a heat treatment workpiece pass-line; a heating device that includes a heating coil disposed downstream of the feed device in the feed direction and encircling the pass-line; a cooling device that is disposed adjacent to the heating coil, downstream of the heating coil in the feed direction, and encircling the pass-line; and a gas supply device that is disposed upstream of the heating coil in the feed direction, directly connected to the heating coil and encircling the pass-line, and that includes a plurality of gas compartments configured by internally partitioning the gas supply device in the feed direction.

Abstract: “A method for thermally treating a flat steel product, a thermally treated flat steel product and use thereof. The method includes providing a flat steel product with a structure with a first hardness. The flat product is heated at least in sections to an austenitizing temperature. The heated flat product is cooled at least in sections so that a structure with a second hardness is formed within the flat product at least in sections, the second hardness having a higher level of hardness in comparison to the structure with the first hardness. The heating and the cooling down of the flat product are coordinated with each other such that the structure with the second hardness is formed across the thickness of the flat product and at least in one of said sections, the structure with the first hardness remains constant across the thickness of the flat product.

Abstract: One aspect of the present invention is a high strength steel sheet having a specific component composition, wherein a metal structure of the steel sheet comprises polygonal ferrite, bainite, tempered martensite, and retained austenite; when the metal structure is observed with a scanning electron microscope, the metal structure satisfies polygonal ferrite: 10 to 50 area %, bainite: 10 to 50 area %, and tempered martensite: 10 to 80 area % with respect to the metal structure overall; and when the metal structure is measured by X-ray diffractometry, the metal structure satisfies retained austenite: 5.0 volume % or more, retained austenite with a carbon concentration of 1.0 mass % or less: 3.5 volume % or more, and retained austenite with a carbon concentration of 0.8 mass % or less: 2.4 volume % or less with respect to the metal structure overall.

Abstract: Provided herein is a plated steel sheet having a yield ratio, strength (tensile strength), elongation, hole expansibility, and CTS. A method for producing the plated steel sheet is also provided, among others. A steel sheet of a specific composition is provided that has a micro structure containing 70 to 90% of ferrite, 5 to 20% of martensite, 5% or less of retained austenite, 10% or less of bainite, and 5% or less of perlite by volume. The ferrite has an average crystal grain diameter of 20 ?m or less. The martensite has an average crystal grain diameter of 5 ?m or less. The retained austenite has an average crystal grain diameter of 5 ?m or less. The bainite has an average crystal grain diameter of 7 ?m or less. The steel sheet has a tensile strength of 590 MPa or more.

Abstract: This invention relates to methods for the recovery of precious metals such as gold, silver, platinum, palladium and rare earths from precious metal-bearing materials.

Abstract: Provided is an improved composition of a nickel-based superalloy. The improved composition may have Ni-8Cr-10Co-0.6Mo-8Ta-1.25Re-5.7Al-OTi-0.1Hf-0.25Si-0.008B-0.0207C-0.02Y.

Abstract: Provided is an aluminum alloy element for a slide fastener, which has improved strength and abrasion resistance. The element for the slide fastener includes a base material of an aluminum alloy having a composition represented by a general formula: AlaSibCucMgdTieBf in which a, b, c, d, e and f each represents % by mass; a is a balance; 0.2?b?0.8, 0.8?c?1.8, 0.8?d?1.8, 0<e?0.05, and 0<f?0.01; and unavoidable impurity elements may be contained; the aluminum alloy comprising, dispersed therein, precipitates containing at least one element selected from a group consisting of Al, Si, Cu and Mg, the element for the slide fastener comprising a pair of leg portions and a head portion that connects the pair or leg portions and comprises a convex portion and a concave portion for engagement.