Patents Issued in June 11, 2019
  • Polypeptide having protease activity and methods for increasing its activity thereof
    Patent number: 10316310
    Abstract: The present invention relates to a polypeptide having protease activity comprising a zinc finger protease domain, a helix-turn-helix domain and a GAF domain. The core protein sequence of the protease is shown as SEQ ID NO: 1. The invention also relates to optimized reaction conditions for the protease and methods of increasing the protease activity.
    Type: Grant
    Filed: December 13, 2016
    Date of Patent: June 11, 2019
    Assignee: Zhejiang University
    Inventors: Yuejin Hua, Yunguang Wang, Liangyan Wang
  • Antibody-urease conjugates
    Patent number: 10316311
    Abstract: This disclosure provides antibody-urease conjugates having therapeutic and diagnostic utility. More specifically, the disclosure relates to diagnostic and/or therapeutic conjugates that are prepared by conjugating one or more whole antibodies to urease.
    Type: Grant
    Filed: April 3, 2014
    Date of Patent: June 11, 2019
    Assignee: Helix Biopharma Corp.
    Inventor: Heman Chao
  • Composition and method for delivery of microorganisms in a dry mode in porous structure
    Patent number: 10316312
    Abstract: The present invention generally relates to compositions and methods of delivering substances in a dry mode, wherein the compositions include an inert carrier substrate having a porous structure, microorganisms loaded throughout the pores of the inert carrier substrate, and a surface layer disposed on the outer surface of the inert carrier substrate, wherein the surface layer is permeable to molecules that aid in cell growth of the microorganisms such that the composition is operable to allow for increased propagation of the microorganisms within the pores of the inert carrier substrate as compared to another composition having an absence of the surface layer.
    Type: Grant
    Filed: October 5, 2011
    Date of Patent: June 11, 2019
    Assignee: DRYLET, LLC
    Inventors: Ramiro Trevino, Steven R. Ellis
  • Mesoporous catalysts of magnetic nanoparticles and free-radical-producing enzymes, and methods of use
    Patent number: 10316313
    Abstract: A composition comprising mesoporous aggregates of magnetic nanoparticles and free-radical producing enzyme (i.e., enzyme-bound mesoporous aggregates), wherein the mesoporous aggregates of magnetic nanoparticles have mesopores in which the free-radical-producing enzyme is embedded. Methods for synthesizing the enzyme-bound mesoporous aggregates are also described. Processes that use said enzyme-bound mesoporous aggregates for depolymerizing lignin, removing aromatic contaminants from water, and polymerizing monomers polymerizable by a free-radical reaction are also described.
    Type: Grant
    Filed: December 15, 2016
    Date of Patent: June 11, 2019
    Assignee: Cornell University
    Inventors: Stephane C. Corgie, Patarawan Kahawong, Emmanuel P. Giannelis, Larry P. Walker
  • Nucleic acid extraction method
    Patent number: 10316314
    Abstract: A nucleic acid extraction method includes the following steps. Firstly, a mixture of a sample and a lysis buffer is provided. The pH of the lysis buffer is lower than 7. Then, the mixture of the sample and the lysis buffer is transferred to a nucleic acid catching chamber containing chitosan coating material, so that nucleic acid of the sample is bound to the chitosan coating material. Then, the chitosan coating material is washed with at least one wash buffer, thereby removing residual protein and cell debris. Then, the chitosan coating material is eluted with an elution buffer while heating the chitosan coating material, so that the nucleic acid is separated from the chitosan coating material. The pH of the elution buffer is higher than 7.
    Type: Grant
    Filed: August 20, 2015
    Date of Patent: June 11, 2019
    Assignee: DELTA ELECTRONICS INT'L (SINGAPORE) PTE LTD
    Inventors: Revata Utama, Yong Zhang, Song Bin Huang, Wang Chu Chen
  • Methods for selecting binders by phage display and masked selection
    Patent number: 10316315
    Abstract: The present invention relates to methods for selecting binders by phage display and masked selection. More particularly, the present invention relates to a method for selecting a plurality of binders specific for at least one relevant target comprising screening a phage binder library of binders against the relevant target in presence of a plurality of binders obtained from a library of binders directed against at least one irrelevant target and positively selecting the binders that are specific for the at least one relevant target.
    Type: Grant
    Filed: February 8, 2017
    Date of Patent: June 11, 2019
    Assignees: INSERM (INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE), UNIVERSITE D'AIX-MARSEILLE, INSTITUT JEAN PAOLI & IRENE CALMETTES, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)
    Inventors: Daniel Baty, Patrick Chames
  • Compositions for targeted delivery of siRNA
    Patent number: 10316316
    Abstract: The present invention is directed compositions for targeted delivery of RNA interference (RNAi) polynucleotides to hepatocytes in vivo. Targeted RNAi polynucleotides are administered together with co-targeted delivery polymers. Delivery polymers provide membrane penetration function for movement of the RNAi polynucleotides from outside the cell to inside the cell. Reversible modification provides physiological responsiveness to the delivery polymers.
    Type: Grant
    Filed: April 19, 2016
    Date of Patent: June 11, 2019
    Assignee: Arrowhead Pharmaceuticals, Inc.
    Inventors: David L Lewis, David B Rozema, Darren H Wakefield, Torsten Hoffman, Erik Kitas, Peter Mohr, Philipp Hadwiger, Wilma Thuer, Linda Valis
  • Treatment of adiponectin (ADIPOQ) related diseases by inhibition of natural antisense transcript to an adiponectin (ADIPOQ)
    Patent number: 10316317
    Abstract: The present invention relates to antisense oligonucleotides that modulate the expression of and/or function of an Adiponectin (ADIPOQ), in particular, by targeting natural antisense polynucleotides of an Adiponectin (ADIPOQ). The invention also relates to the identification of these antisense oligonucleotides and their use in treating diseases and disorders associated with the expression of Adiponectins (ADIPOQ)s.
    Type: Grant
    Filed: March 6, 2018
    Date of Patent: June 11, 2019
    Assignee: CuRNA, Inc.
    Inventors: Joseph Collard, Olga Khorkova Sherman
  • Oligonucleotide compositions and uses thereof
    Patent number: 10316318
    Abstract: The present invention provides oligonucleotide inhibitors of miR-155 and compositions thereof. The invention further provides methods for treating cancer such as a T cell lymphoma in a subject by administering to the subject an oligonucleotide inhibitor of miR-155. The invention also provides methods for reducing or inhibiting the proliferation of malignant T cells by administering an oligonucleotide inhibitor of miR-155.
    Type: Grant
    Filed: May 10, 2018
    Date of Patent: June 11, 2019
    Assignee: MIRAGEN THERAPEUTICS, INC.
    Inventors: David Rodman, Anita Seto, Christina Dalby, Aimee Jackson, Xuan Beatty
  • Composition for diagnosis of liver metastasis of colorectal cancer and the use thereof
    Patent number: 10316319
    Abstract: The present disclosure relates to a composition for diagnosis of liver metastasis of colorectal cancer and the use thereof, and more particularly to a composition for diagnosis of liver metastasis of colorectal cancer, which comprises either a substance for measuring the mRNA level of CCL7 (Chemokine (C-C motif) ligand 7) gene or a substance for measuring the level of a protein which is encoded by the gene. According to the present disclosure, whether liver metastasis of colorectal cancer occurred can be diagnosed by measuring the mRNA expression level of the CCL7 gene or the expression level of the CCL7 protein, and the use of the composition comprising an inhibitor of CCL7 gene allows the treatment of colorectal cancer or the liver metastasis of colorectal cancer.
    Type: Grant
    Filed: November 9, 2016
    Date of Patent: June 11, 2019
    Assignee: SAMSUNG LIFE PUBLIC WELFARE FOUNDATION
    Inventors: Woo Yong Lee, Yong Beom Cho, Ho-Kyung Chun
  • Systems comprising non-immunogenic and nuclease resistant nucleic acid origami devices for molecular computation
    Patent number: 10316320
    Abstract: Systems acting as logic gates and systems exhibiting quorum sensing are provided, wherein said systems comprise at least one effector nucleic acid origami device, at least one regulator nucleic acid origami device, at least one input and at least one output, and the nucleic acid origami devices are non-immunogenic and/or resistant to nucleases.
    Type: Grant
    Filed: April 13, 2014
    Date of Patent: June 11, 2019
    Assignee: BAR ILAN UNIVERSITY
    Inventors: Ido Bachelet, Almogit Abu-Horowitz, Eldad Ben-Ishay, Yaniv Amir, Joel De Beer, Shmulik Ittah
  • Method for generating aptamers with improved off-rates
    Patent number: 10316321
    Abstract: The present disclosure describes improved SELEX methods for producing aptamers that are capable of binding to target molecules and improved photoSELEX methods for producing photoreactive aptamers that are capable of both binding and covalently crosslinking to target molecules. Specifically, the present disclosure describes methods for producing aptamers and photoaptamers having slower dissociation rate constants than are obtained using prior SELEX and photoSELEX methods. The disclosure further describes aptamers and photoaptamers having slower dissociation rate constants than those obtained using prior methods. In addition, the disclosure describes aptamer constructs that include a variety of functionalities, including a cleavable element, a detection element, and a capture or immobilization element.
    Type: Grant
    Filed: January 23, 2018
    Date of Patent: June 11, 2019
    Assignee: SomaLogic Inc.
    Inventors: Dominic Zichi, Sheri K. Wilcox, Chris Bock, Daniel J. Schneider, Bruce Eaton, Larry Gold
  • High growth capacity auxotrophic and methods of use
    Patent number: 10316322
    Abstract: This invention provides high growth capacity strains of auxotrophic Escherichia coli and methods for generating thereof. The high growth capacity strains express a complementing auxotrophic plasmid that allows the strain to grow in the absence of the auxotrophic amino acid. Also, provided herein is a method for preparing a bacterial cell extract of a high growth capacity strain of auxotrophic Escherichia coli for use in an in vitro protein expression.
    Type: Grant
    Filed: June 30, 2015
    Date of Patent: June 11, 2019
    Assignee: Sutro Biopharma, Inc.
    Inventors: Dan Groff, Patrick Rivers, Stuart Bussell, Alexander Steiner
  • Microorganisms engineered to use unconventional sources of nitrogen
    Patent number: 10316323
    Abstract: Disclosed are genetically engineered organisms, such as yeast and bacteria, that have the ability to metabolize atypical nitrogen sources, such as melamine and cyanamide. Fermentation methods using the genetically engineered organisms are also described. The methods of the invention are robust processes for the industrial bioproduction of a variety of compounds, including commodities, fine chemicals, and pharmaceuticals.
    Type: Grant
    Filed: August 17, 2017
    Date of Patent: June 11, 2019
    Assignee: Novogy, Inc.
    Inventors: Colin South, Arthur J Shaw, IV
  • Compositions and methods for modifying genomes
    Patent number: 10316324
    Abstract: Compositions and methods for modifying genomic DNA sequences are provided. The methods produce double-stranded breaks (DSBs) at pre-determined target sites in a genomic DNA sequence, resulting in mutation, insertion, and/or deletion of DNA sequences at the target site(s) in a genome. Compositions comprise DNA constructs comprising nucleotide sequences that encode a Cms1 protein operably linked to a promoter that is operable in the cells of interest. The DNA constructs can be used to direct the modification of genomic DNA at pre-determined genomic loci. Methods to use these DNA constructs to modify genomic DNA sequences are described herein. Additionally, compositions and methods for modulating the expression of genes are provided.
    Type: Grant
    Filed: August 8, 2018
    Date of Patent: June 11, 2019
    Assignee: Benson Hill Biosystems, Inc.
    Inventors: Matthew Begemann, Benjamin Neil Gray
  • Nitrogen uptake in plants
    Patent number: 10316325
    Abstract: This disclosure provides transgenic plants, including crop plants and methods for their production. The transgenic plants comprise recombinant DNA constructs for the expression of polypeptides encoded by the DNA constructs. The polypeptides are capable of conferring improved traits to the transgenic plants when expressed under the control of a tissue-enhanced promoter. This disclosure also pertains to transgenic plants and their progeny plants, wherein the transgenic or progeny plants comprise the recombinant DNA constructs and are selected for having enhanced nitrogen use efficiency and/or nitrogen uptake. Seed of the transgenic plants that can be grown into a plant that comprise the disclosed recombinant DNA constructs and exhibits having enhanced nitrogen use efficiency, and which may be selected for this trait, are also envisioned.
    Type: Grant
    Filed: March 11, 2015
    Date of Patent: June 11, 2019
    Assignee: Koch Biological Solutions, LLC
    Inventors: Robert A. Creelman, Suqin Cai, Hans E. Holtan, T. Lynne Reuber, Oliver J. Ratcliffe
  • Compositions and methods comprising sequences having hydroxyphenylpyruvate dioxygenase (HPPD) activity
    Patent number: 10316326
    Abstract: Compositions and methods comprising polynucleotides and polypeptides having 4-hydroxyphenylpyruvate dioxygenase (HPPD) activity and having insensitivity to an HPPD inhibitor are provided. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the HPPD sequences. Various methods of employing the HPPD sequences are provided. Such methods include, for example, methods for producing an HPPD inhibitor tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein. Methods are also provided to identify additional HPPD variants. Further provided are various methods and compositions that allow the various HPPD polypeptides and variant and fragments thereof to be expressed in a chloroplast or transported to a chloroplast.
    Type: Grant
    Filed: May 2, 2015
    Date of Patent: June 11, 2019
    Assignee: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: Daniel Siehl, Phillip A. Patten, Yumin Tao, Ericka Bermudez, Linda A. Castle, Yuxia Dong, Andrew Duncan Satterfield, Henrik Albert
  • Genetically modified plants that are insect-resistant and/or rot resistant
    Patent number: 10316327
    Abstract: An insect-resistant and/or fungi-resistant transgenic plant. The plant may be transformed with a gene construct including at least one gene chosen from LECRPA1, LECRPA2, and LECRPA3.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: June 11, 2019
    Inventors: Yi Li, Lisa Hollister
  • Melon plants with improved traits
    Patent number: 10316328
    Abstract: The present disclosure provides melon plants exhibiting resistance to powdery mildew, aphids, and cucumber vein yellowing virus (CVYV). In certain embodiments, the invention provides melon plants with powdery mildew, aphid, and CVYV resistance loci located in cis linkage on chromosome 5. In certain aspects, compositions, including novel polymorphic markers and methods for producing, breeding, identifying, and selecting plants or germplasm with a disease resistance phenotype are provided.
    Type: Grant
    Filed: August 11, 2017
    Date of Patent: June 11, 2019
    Assignee: SEMINIS VEGETABLE SEEDS, INC.
    Inventors: Eleni Bachlava, Alfonso Cuesta-Marcos, Jeffrey M. Mills, Kelli M. Durham, Robyn L. Morgan, Tarek Joobeur, Susana Garcia Andrés
  • Proteins toxic or inhibitory to lepidopteran insects
    Patent number: 10316329
    Abstract: Nucleotide sequences are disclosed encoding novel, insecticidal TIC2160 and TIC3244 proteins, and variants thereof, and related proteins exhibiting Lepidopteran inhibitory activity, as well as fragments thereof. Particular embodiments provide compositions and transformed plants, plant parts, and seeds containing a polynucleotide construct encoding one or more of the toxin proteins within the TIC2160-related protein toxin class.
    Type: Grant
    Filed: October 15, 2015
    Date of Patent: June 11, 2019
    Assignee: Monsanto Technology LLC
    Inventors: James Arthur Baum, David Joseph Bowen, Catherine Alice Chay, Artem G Evdokimov, Stanislaw Flasinski, Uma Rao Kesanapalli, Jeffrey R Nageotte, James Kevin Roberts, Brian Edward Weiner
  • Corn event MON 87411
    Patent number: 10316330
    Abstract: The invention provides corn event MON 87411, and plants, plant cells, seeds, plant parts, and commodity products comprising event MON 87411. The invention also provides polynucleotides specific for event MON 87411 and plants, plant cells, seeds, plant parts, and commodity products comprising polynucleotides specific for event MON 87411. The invention also provides methods related to event MON 87411.
    Type: Grant
    Filed: July 28, 2016
    Date of Patent: June 11, 2019
    Assignee: Monsanto Technology LLC
    Inventors: Wen C. Burns, Catherine A. Chay, Cheryl L. Cloninger, Mingqi Deng, Stanislaw Flasinski, Kunsheng Wu
  • Insect inhibitory toxin family active against Hemipteran and/or Lepidopteran insects
    Patent number: 10316331
    Abstract: The present invention discloses a genus of insect inhibitory proteins that exhibit properties directed to controlling Lepidopteran and/or Hemipteran crop pests, methods of using such proteins, nucleotide sequences encoding such proteins, methods of detecting and isolating such proteins, and their use in agricultural systems.
    Type: Grant
    Filed: April 13, 2017
    Date of Patent: June 11, 2019
    Assignee: Monsanto Technology LLC
    Inventors: David J. Bowen, Catherine Chay, Artem Evdokimov, Stanislaw Flasinski, Uma R. Kesanapalli, Megan N. Schroder, Rachael N. Slightom, Nengbing Tao, Andrew M. Wollacott
  • Cell for immunotherapy, including modified nucleic acid construct encoding Wilms tumor gene product
    Patent number: 10316332
    Abstract: A cell for immunotherapy of the present invention includes a nucleic acid encoding a Wilms tumor 1 gene product or a fragment of the Wilms tumor 1 gene product, wherein the nucleic acid including (i) a region encoding a fragment of the Wilms tumor 1 gene product, the fragment being indicated by positions 194 to 493 of amino acid sequence of SEQ ID NO:1 or by positions corresponding to the positions 194 to 493 of amino acid sequence corresponding to SEQ ID NO:1 and (ii) only one AUG as a functional start codon, connected to a 5? terminal side of the region via 3m (m is 124-192) bases intervening between the AUG as the functional start codon and the 5? terminal side of the region, and a nucleic acid encoding CD1d, wherein the cell has been loaded with a glycolipid recognized by antigen receptor of NKT cell.
    Type: Grant
    Filed: February 27, 2018
    Date of Patent: June 11, 2019
    Assignee: RIKEN
    Inventor: Shin-ichiro Fujii
  • Producer cells for replication competent retroviral vectors
    Patent number: 10316333
    Abstract: The disclosure provide cell lines and methods for the production of vectors and viral particles useful in gene therapy.
    Type: Grant
    Filed: June 17, 2010
    Date of Patent: June 11, 2019
    Assignee: Tocagen Inc.
    Inventors: Douglas J. Jolly, Carlos Ibanez
  • Cytomegalovirus vectors enabling control of T cell targeting
    Patent number: 10316334
    Abstract: CMV vectors comprising a heterologous protein antigen, an active UL131 protein (or an ortholog thereof), and an active UL128 protein (or an ortholog thereof) but lacking an active UL130 protein (or an ortholog thereof) are provided. CMV vectors comprising a heterologous protein antigen, an active UL131 protein (or an ortholog thereof), and an active UL130 protein (or an ortholog thereof) but lacking an active UL128 protein are also provided. In addition, methods of using CMV vectors to generate an immune response characterized as having at least 10% of the CD8+ T cells directed against epitopes presented by MHC Class II are provided.
    Type: Grant
    Filed: September 1, 2017
    Date of Patent: June 11, 2019
    Assignee: Oregon Health & Science University
    Inventors: Louis Picker, Scott Hansen, Klaus Frueh, Daniel Malouli
  • Methods and compositions for generating stable transfected cells
    Patent number: 10316335
    Abstract: Methods and compositions are provided involving high producing cell lines. Embodiments concern efficient methods for screening for such cell lines and for creating such cell lines. These cell lines can be used to create large amounts of protein. To quickly generate large quantity of recombinant proteins or vaccines for both pre-clinical study and clinical trials, almost all drug development will face the same challenging obstacle of rapidly generating a high stable producer. Developing and identifying a stable cell line is a critical part of biopharmaceutical development.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: June 11, 2019
    Assignee: MaxCyte, Inc.
    Inventors: Weili Wang, James P. Brady, Madhusudan V. Peshwa
  • Systems and methods for continuously fermenting C5 and C6 saccharides
    Patent number: 10316336
    Abstract: This invention provides optimized fermentation of cellulosic and hemicellulosic sugars. Biomass-derived hemicellulosic and cellulosic sugars are independently conditioned and separately fermented, utilizing reuse and recycle of microorganisms, metabolic intermediates, and nutrients. Conditioned sugars can be fermented in separate vessels, where excess cells from glucose fermentation are conveyed to hemicellulose sugar fermentation along with raffinate from solvent recovery, to enhance productivity and product yield.
    Type: Grant
    Filed: May 25, 2017
    Date of Patent: June 11, 2019
    Assignee: API Intellectual Property Holdings, LLC
    Inventors: Shrikant Survase, Ryan Zebroski, Georgios Adamos, Vesa Pylkkanen
  • Genetically engineered bacterium for the production of isobutylene
    Patent number: 10316337
    Abstract: The invention relates to a genetically engineered bacterium having an enzyme that converts 3-hydroxyisovaleryl-CoA to 3-hydroxyisovalerate and an enzyme that converts 3-hydroxyisovalerate to isobutylene. Typically, the bacterium is capable of producing isobutylene from a gaseous substrate containing CO, CO2, and/or H2, such as syngas or an industrial waste gas.
    Type: Grant
    Filed: March 15, 2018
    Date of Patent: June 11, 2019
    Assignee: LANZATECH NEW ZEALAND LIMITED
    Inventors: Michael Koepke, Rasmus Overgaard Jensen
  • Enzymatic process for the preparation of (1S,2R)-2-(difluoromethyl)-1-(propoxycarbonyl)cyclopropanecarboxylic acid
    Patent number: 10316338
    Abstract: Disclosed are methods of synthesizing enantioenriched difluoroalkylcyclopropyl amino esters and their salts, such as the dicyclohexylamine salt of (1S,2R)-2-(difluoromethyl)-1-(propoxycarbonyl)cyclopropane carboxylic acid. These compounds are useful intermediates in the synthesis of viral protease inhibitors.
    Type: Grant
    Filed: January 29, 2016
    Date of Patent: June 11, 2019
    Assignee: Abb Vie Inc.
    Inventors: Michael J. Abrahamson, Angelica B. Kielbus, William T. Riordan, David R. Hill, Sanjay R. Chemburkar, Rajarathnam E. Reddy, Timothy B. Towne, Jianzhang Mei, Gareth J. Brown, Stefan Mix
  • Microorganisms for production of -succinylhomoserine and method for production of -succinylhomoserine using the same
    Patent number: 10316339
    Abstract: The present invention relates to a polypeptide having a resistant to feedback inhibition by methionine and an activity of homoserine O-succinyltransferase, a O-succinyl homoserine-producing microorganism expressing the polypeptide, and a method for producing O-succinyl homoserine using the same.
    Type: Grant
    Filed: October 22, 2014
    Date of Patent: June 11, 2019
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Su Jin Choi, So Young Kim, Chang Il Seo, Yong Uk Shin, Young Lyeol Yang, Hye Won Um, Hye Min Park, Sung Hoo Jhon, Byung Hoon Jung
  • Glucose production method and glucose produced by said method
    Patent number: 10316340
    Abstract: A glucose production method is characterized in that glucose is produced from organisms that hold at least a disaccharide inside the body, which organisms utilize a cellulose source as food or for nutrients, particularly insects, crustaceans, and mushrooms.
    Type: Grant
    Filed: June 2, 2014
    Date of Patent: June 11, 2019
    Assignees: ACTEIIVE CORPORATION
    Inventors: Kengo Sakaguchi, Yoshihiro Kanai, Tatsushi Ruike
  • Process for the hydrolysis of lignocellulosic material, wherein the hydrolysate is used for microbial hydrolase production
    Patent number: 10316341
    Abstract: The present invention is directed to a process for self-sufficient hydrolysis of lignocellulosic material. In an additional aspect, the present invention is directed to a process for the production of an organic product and the organic product produced according to this process.
    Type: Grant
    Filed: May 20, 2015
    Date of Patent: June 11, 2019
    Assignee: Clariant International Ltd.
    Inventors: Michael Zavrel, Danielle Dennewald, Joerg Bartuch, Henning Marckmann
  • Cell-free production of sugars
    Patent number: 10316342
    Abstract: Provided herein, in some embodiments, are systems, methods, and compositions (e.g., cells and cell lysates) for enzymatically converting a polymeric glucose carbohydrate (e.g., starch) to sugar.
    Type: Grant
    Filed: July 12, 2018
    Date of Patent: June 11, 2019
    Assignee: GreenLight Biosciences, Inc.
    Inventors: Daniel MacEachran, Drew S. Cunningham, William Jeremy Blake, Matthew Eduardo Moura
  • Compositions comprising a polypeptide having cellulolytic enhancing activity and a liquor and uses thereof
    Patent number: 10316343
    Abstract: The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and a liquor. The present invention also relates to methods of using the compositions.
    Type: Grant
    Filed: June 7, 2016
    Date of Patent: June 11, 2019
    Assignees: Novozymes, Inc., Novozymes North America, Inc.
    Inventors: Jason Quinlan, Feng Xu, Matt Sweeney, Don Higgins, Hui Xu
  • Methods for degrading or converting plant cell wall polysaccharides
    Patent number: 10316344
    Abstract: The present invention relates to methods for converting plant cell wall polysaccharides into one or more products, comprising: treating the plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into the one or more products.
    Type: Grant
    Filed: June 15, 2016
    Date of Patent: June 11, 2019
    Assignee: Novozymes, Inc.
    Inventors: Randy Berka, Joel Cherry
  • Analysis of nucleic acids associated with single cells using nucleic acid barcodes
    Patent number: 10316345
    Abstract: Provided herein are methods and compositions for analyzing nucleic acids associated with single cells using nucleic acid barcodes. According to some embodiments, a method for producing one or more polynucleotides of interest comprises: obtaining a plurality of RNAs associated with one or more samples, wherein the samples are obtained from one or more subjects, each RNA is associated with a single sample, and the RNAs associated with each sample are present in a separate reaction volume; adding an adapter molecule to the RNAs associated with each sample, wherein the adapter molecule is generated using an enzymatic reaction and comprises a universal priming sequence, a barcode sequence, and a binding site; and incorporating the barcode sequence into one or more polynucleotides associated with each sample, thereby producing the one or more polynucleotides of interest.
    Type: Grant
    Filed: February 8, 2017
    Date of Patent: June 11, 2019
    Assignee: ATRECA, INC.
    Inventors: Yann Chong Tan, Gary Withey
  • Method and apparatus for dispersion of microbes in a liquid suspension
    Patent number: 10316346
    Abstract: An apparatus for dispersing microbes in a biological sample such as a liquid suspension sample and measuring turbidity of the liquid suspension is disclosed. The apparatus is configured for dispersing the microbes in a liquid suspension without destroying the integrity of the microbes and measure the turbidity of the sample suspension at the same time or in the same sample set up without having to move the microbe sample from one equipment to another.
    Type: Grant
    Filed: September 25, 2015
    Date of Patent: June 11, 2019
    Inventors: Guofeng Zhu, Baizi Mo, Hongbo Zhang, Jian Hou, Weiguo Zhao, Lijun Bi, Jiaoyu Deng, Shengce Tao, Haiying Hang, Jiawei Li, Xiao Yang
  • Endospore detection using hydrophobic collection material
    Patent number: 10316347
    Abstract: In situ optical analysis of bacterial endospores can be inhibited when the endospores are present within an optically active carrier medium. To help isolate the endospores from the carrier medium, in some examples, the carrier medium is passed through a hydrophobic material that captures the endospores via hydrophobic attraction. Subsequently, a germination fluid and lanthanide source, such as terbium, can be added to the bacterial endospores captured on the hydrophobic material to form a lanthanide-dipicolinic acid complex in the germination fluid. The germination fluid can then be optically analyzed by measuring the fluorometric response of the lanthanide-dipicolinic acid complex to determine a concentration of the bacterial endospores in the carrier medium.
    Type: Grant
    Filed: June 26, 2014
    Date of Patent: June 11, 2019
    Assignee: Ecolab USA Inc.
    Inventors: Anthony Wayne Erickson, Elaine Patricia Black, Nathan Richard Ortmann, Rodney H. Banks
  • Diagnostic system and process for rapid bacterial infection diagnosis
    Patent number: 10316348
    Abstract: Methods and devices for monitoring the viability of a biofilm comprising Pseudomonas Aeruginosa bacteria are provided by detecting pyocyanin. The invention relates to electrochemical methods and devices that offer a simple and inexpensive alternative for immediate identification of bacterial infection due to the presence of Pseudomonas aeruginosa. In some embodiments, an inexpensive, disposable electrochemical sensor can be used to rapidly screen for the presence of P. aeruginosa in clinical wound effluent samples.
    Type: Grant
    Filed: September 16, 2015
    Date of Patent: June 11, 2019
    Assignee: Northeastern University
    Inventors: Edgar D. Goluch, Hunter J. Sismaet, Thaddaeus A. Webster
  • Use of aptamers in proteomics
    Patent number: 10316349
    Abstract: The present invention is a method for measuring the amount of at least one molecule in a biological sample, the method comprising a) combining the sample, or a derivative thereof, with one or more aptamers and allowing one or more molecules in the sample to bind to the aptamer(s); b) separating bound from unbound molecules; and c) quantifying the molecule(s) bound to the or each aptamer, wherein quantification of the bound molecule(s) is carried out by sequencing at least part of the or each aptamer. Uses of and products derived from the method are also contemplated.
    Type: Grant
    Filed: August 4, 2017
    Date of Patent: June 11, 2019
    Assignee: Caris Science, Inc.
    Inventors: Clive Gavin Brown, Koen Kas, Sven Agnes Jan Eyckerman
  • Pretreatment method and nucleic-acid-extracting kit usable for the same
    Patent number: 10316350
    Abstract: The present invention provides a method capable of realizing pretreatment process of genetic screening according to a POCT mode. The method includes: making a sample, extraction liquid for extracting nucleic-acid contained in the sample, silica particles, and a filtering material contact with each other; making the filtering material carry composite material of the nucleic-acid and the silica particles thereon; and then delivering the filtering material to a nucleic-acid-amplifying process by means of reaction solution for amplifying nucleic-acid, wherein particle diameters of the silica particles and concentration of the silica particles in the reaction solution for amplifying nucleic-acid are set up within a predetermined range.
    Type: Grant
    Filed: September 2, 2015
    Date of Patent: June 11, 2019
    Assignee: MIZUHO MEDY CO., LTD.
    Inventors: Kazutomi Yamakawa, Takashi Nagano
  • DNA quality assessments using repetitive sequences
    Patent number: 10316351
    Abstract: The present disclosure provides methods, arrays and kits for assessing the quality of genomic DNA samples, especially those obtained from formalin-fixed paraffin-embedded (FFPE) samples. The methods, arrays and kits provided herein use primer pairs specific to regions in the genomes of the organisms from which genomic DNA samples are obtained that have identical or nearly identical copies distributed across multiple chromosomes.
    Type: Grant
    Filed: March 13, 2018
    Date of Patent: June 11, 2019
    Assignee: QIAGEN Sciences, LLC
    Inventors: Yexun Wang, John DiCarlo, Vikram Devgan, Qiong Jiang, Quan Peng
  • Methods of capturing a target nucleic acid for amplification and detection using an inactivatable target capture oligomer
    Patent number: 10316352
    Abstract: The present invention provides compositions, kits and methods for the selective hybridization and capture of a specific target nucleic acid. The specific target nucleic acid may be present in a heterogeneous mixture of nucleic acids. Selective hybridization and capture provides a target nucleic acid that is substantially free of non-target and/or contaminating nucleic acids. Target nucleic acids that have been selectively hybridized and captured using the current invention are then used in subsequent analysis, wherein the presence of non-target and/or contaminating nucleic acids that interfere with said subsequent analysis have been substantially reduced or eliminated, thereby providing improved analysis results.
    Type: Grant
    Filed: May 13, 2009
    Date of Patent: June 11, 2019
    Assignee: GEN-PROBE INCORPORATED
    Inventors: Michael M. Becker, Kristin W. Livezey, Wai-Chung Lam
  • Sequence conversion and signal amplifier DNA having poly DNA spacer sequences and detection methods using same
    Patent number: 10316353
    Abstract: Disclosed are methods for detecting a target nucleic acid in a sample. The methods include contacting said sample, in the presence of a polymerase and an endonuclease, with a sequence conversion oligonucleotide. Also disclosed are methods for detecting a target nucleic acid in a sample in which said sample is contacted, in the presence of a polymerase and an endonuclease, with a sequence conversion oligonucleotide and a signal amplifier oligonucleotide. The disclosure also provides compositions and kits comprising such sequence conversion and signal amplifier oligonucleotides.
    Type: Grant
    Filed: October 13, 2015
    Date of Patent: June 11, 2019
    Assignees: Abbott Laboratories, Tokyo Institute of Technology
    Inventors: Ken Komiya, Makoto Komori, Toru Yoshimura
  • Electrochemical substrate patterning and analyte detection on a two-electrode platform
    Patent number: 10316354
    Abstract: A two-electrode detection system having target substrates including nucleic acids, proteins, and/or small molecules on specifically defined regions of a single surface. The spatial distribution of the target substrate on the surface allows for more accurate substrate interactions and analysis. Additionally, the detection system of the present invention allows for patterning of different target substrates, thereby affording more accurate analysis of multiple substrate targets.
    Type: Grant
    Filed: July 14, 2014
    Date of Patent: June 11, 2019
    Assignee: California Institute of Technology
    Inventors: Ariel L. Furst, Michael G. Hill, Natalie B. Muren, Jacqueline K. Barton
  • Nanopipette analysis of polymers
    Patent number: 10316355
    Abstract: The disclosure relates to devices and instruments for detecting and individually analyzing biomolecules, biomolecular complexes and biomolecules with ligands attached thereon.
    Type: Grant
    Filed: July 24, 2015
    Date of Patent: June 11, 2019
    Inventor: Kalim Mir
  • Method of constructing sequencing library with bubble-shaped adaptor element
    Patent number: 10316356
    Abstract: Provided is a method of constructing a sequencing library using an adaptor element in a bubble shape. The bubble-shaped adaptor is ligated to a DNA fragment respectively at the 3?-terminal and the 5?-terminal i.e., two bubble-shaped adaptors with same sequences are ligated in one step. The bubble-shaped adaptor-ligated product is then amplified with a primer complementary to the 3?-terminal of the long-chain nucleic acid of the bubble-shaped adaptor, so as to replace the non-paired sequence in the short-chain nucleic acid.
    Type: Grant
    Filed: November 21, 2014
    Date of Patent: June 11, 2019
    Assignee: MGI TECH CO., LTD.
    Inventors: Yuan Jiang, Kai Tian, Xia Zhao, Wenwei Zhang, Huaiqian Xu, Hui Jiang, Radoje Drmanac, Chunyu Geng
  • Compositions and methods for enhanced adapter ligation
    Patent number: 10316357
    Abstract: Compositions and methods for enhanced adapter ligation are provided. The compositions and methods can be useful for next generation sequencing (NGS) library preparation and can result in higher yields in an NGS library.
    Type: Grant
    Filed: July 29, 2016
    Date of Patent: June 11, 2019
    Assignee: Swift Biosciences, Inc.
    Inventors: Vladimir Makarov, Julie Laliberte
  • Oscillating amplification reaction for nucleic acids
    Patent number: 10316358
    Abstract: One embodiment of the present invention provides for a method for amplifying a template of nucleic acid target sequence contained in a sample. The method includes contacting the sample with an amplification reaction mixture containing a primer complementary to the template of nucleic acid target sequence. A temperature of the reaction is oscillated between an upper temperature and a lower temperature wherein the change in temperature is no greater than about 20° C. during a plurality of temperature cycles. The template of nucleic acid target sequence is amplified.
    Type: Grant
    Filed: August 25, 2016
    Date of Patent: June 11, 2019
    Assignee: Mesa Biotech, Inc.
    Inventors: Hong Cai, Nathan J. Cobb
  • Methods for multiplex PCR
    Patent number: 10316359
    Abstract: Methods for the preparation of PCR reaction mixtures and for performing multiplex PCR amplification which limit the production of non-target amplicons are provided. Production of non-target amplicons is limited because the non-target amplicons have complementary 5? and 3? ends as a result of the target-specific primer designs such that the non-target amplicons can form stable secondary structure during the annealing step.
    Type: Grant
    Filed: August 31, 2016
    Date of Patent: June 11, 2019
    Assignee: Swift Biosciences, Inc.
    Inventors: Vladimir Makarov, Julie Laliberte