Abstract: Provided is a method for detecting a target molecule. The method includes providing a chip, the chip including a nanopore in a membrane that is disposed adjacent to or in proximity to a sensing electrode. A nucleic acid molecule is then directed through the nanopore, the nucleic acid molecule being associated with a reporter molecule. The nucleic acid molecule also includes an address region and a probe region, the reporter molecule being associated with the nucleic acid molecule at the probe region. The reporter molecule is also coupled to a target molecule. While the nucleic acid molecule is directed through the nanopore, the address region can be sequenced to determine a nucleic acid sequence of the address region. The target molecule can then be identified, with the aid of a computer processor, based upon the nucleic acid sequence of the address region.

Abstract: To better understand the biology of hormone receptor-positive and negative breast cancer and to identify methylated gene markers of disease progression, a genome-wide methylation array analysis was performed on 103 primary invasive breast cancers and 21 normal breast samples using the Illumina Infinium HumanMethylation27 array that queried 27,578 CpG loci. Forty CpG loci showed differential methylation specific to either ER-positive or ER-negative tumors. Each of the 40 ER-subtype-specific loci was validated in silico using an independent, publicly available methylome dataset from The Cancer Genome Atlas (TCGA). In addition, 100 methylated CpG loci were identified that were significantly associated with disease progression. Arrays containing the ER-subtype-specific loci and their use in methods of diagnosis and treatment of breast cancer are provided.

Abstract: The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

Abstract: Provided is a sensing apparatus comprising a chip for integrated amplification and sequencing of a template polynucleotide in a sample. The apparatus comprises a chip with at least one ISFET in a well or chamber, amplification means for amplifying the template polynucleotide on a surface of said chip and comprising at least one heating means suitable for conducting amplification of the template polynucleotide at temperatures elevated with respect to room temperature, and sequencing means for sequencing the amplified template polynucleotide in said well or chamber. Methods of use are also provided.

Abstract: The present invention relates to a method for identifying the source of an amplicon, comprising: providing a plurality of pools of amplicons from different sources, wherein the amplicons from different sources are present in more than one pool, and wherein the amplicons in each pool are tagged with a unique pool-specific identifier; sequencing at least part of the amplicons that comprise the pool-specific identifiers; and assigning one or more of the amplicons to corresponding pools and/or sources using the pool-specific identifiers.

Abstract: The present invention relates, in general, to gene expression profiles, and in particular, to a peripheral blood gene expression profile of an environmental exposure, ionizing radiation. The invention further relates to methods of screening patients for radiation exposure based on gene expression profiling and to kits suitable for use in such methods.

Abstract: This invention provides methods and materials for measuring telomere abundance from chromosomes in a sample having telomeres within a pre-determined length range, e.g., short telomeres up to a certain length. The methods can involve a first step of performing a time-limited extension reaction calibrated to produce extension products from a double-stranded chromosomal DNA template of no more than a defined length, and a second step of amplifying, from the extension products, sequences bounded by a sub-telomeric sequence and the anchor sequence, to produce a length-limited telomere sequence product. The abundance of telomeric sequences in this product can be measured, and the measures can be correlated to a variety of indices.

Abstract: The present invention is directed to methods for diagnosing breast cancer, metastasizing breast cancer, or for determining the CTC status in a subject. Moreover, the present invention is concerned with devices and kits for carrying methods of the invention. The methods comprise determining in a sample of a subject suspected to be afflicted with breast cancer the amount of at least one miRNA selected from a recited list of miRNAs, followed by comparing the amount with one or more references, whereby breast cancer is to be diagnosed.

Abstract: Provided herein are kits, compositions, and methods for diagnosing and treating interstitial cystitis (IC) and/or interstitial cystitis/bladder pain syndrome (IC/BPS) based on finding lower levels of certain bacteria in a subject's stool sample (e.g., O. splanchnicus, F. prausnitzii, C. aerofaciens, E. sinensis, L. longoviformis, and R. intestinalis). In certain embodiments, then present invention provides probiotic formulations containing live bacteria (e.g., from O. splanchnicus, F. prausnitzii, C. aerofaciens, E. sinensis, L. longoviformis, and R. intestinalis).

Abstract: The present disclosure provides watermelon plants with a male sterile phenotype and their progeny. Such plants may comprise an introgressed genomic region associated with a male sterile phenotype. In certain aspects, compositions, including distinct polymorphic molecular markers and methods for producing, breeding, identifying, and selecting plants or germplasm with a male sterile phenotype are provided.

Abstract: Compositions and methods useful in identifying and selecting maize plants with increased ear weight and yield are provided herein. The methods use molecular genetic markers to identify and/or select maize plants with increased ear weight and increased yield or to identify and counter-select maize plants with decreased ear weight and decreased yield.

Abstract: Methods of inhibiting S. aureus propagation, and screening for compounds that inhibit S. aureus propagation, are described. A method of inhibiting S. aureus propagation comprises either inhibiting or stabilizing ribosomal binding of a specific S. aureus tRNA in the S. aureus by an amount sufficient to inhibit S. aureus protein expression. A method of screening for compounds useful for inhibiting S. aureus propagation comprises contacting a specific S. aureus tRNA to a ribosome that binds that tRNA in the presence of the test compound and an mRNA that codes for methionine and arginine (i.e., includes the sequence AUGAGA), and then determining whether the compound inhibits the binding of that tRNA.

Abstract: Provided herein are methods for determining the presence or absence of an enteroviruses and parechoviruses in a biological sample. The methods involve identifying the presence or absence of a target nucleic acids from the viruses using direct amplification from a biological sample without a step of extraction of the nucleic acids, but retaining substantially the same specificity and sensitivity of methods assaying extracted nucleic acids. Also provided are methods of diagnosis using the methods provided and compositions and kits for the practice of the methods.

Abstract: An ionic liquid represented by the following chemical formula, [(CH3)3N(CH2)2OH]+[NH2(CH2)3CH(NH2)COO]?, which provides an ionic liquid capable of dissolving cellulose within twenty-four hours.

Abstract: A cutting device for machines for cutting hides and the like, including an oscillating piston provided at its end with a cutting blade, the oscillating piston being actuated pneumatically by way of an inlet and an outlet, an oscillation chamber being defined by the lower and upper stroke limits of the oscillating piston, the device including a supply duct that leads to the oscillation chamber, the supply duct being regulated by a solenoid valve in order to raise the oscillating piston and consequently raise the cutting blade.

Abstract: This invention relates to a method and apparatus for gasifying or liquifying coal. In particular, the method comprises reacting a coal with a molten aluminum or aluminum alloy bath. The apparatus includes a reaction vessel for carrying out the reaction, as well as other equipment necessary for capturing and removing the reaction products. Further, the process can be used to cogenerate electricity using the excess heat generated by the process.

Abstract: Methods and systems for producing direct reduced iron having increased carbon content, comprising: providing a reformed gas stream from a reformer; delivering the reformed gas stream to a carbon monoxide recovery unit to form a carbon monoxide-rich gas stream and a hydrogen-rich gas stream; and delivering the carbon-monoxide-rich gas stream to a direct reduction furnace and exposing partially or completely reduced iron oxide to the carbon monoxide-rich gas stream to increase the carbon content of resulting direct reduced iron. The carbon monoxide-rich gas stream is delivered to one of a transition zone and a cooling zone of the direct reduction furnace. Optionally, the method further comprises mixing the carbon monoxide-rich gas stream with a hydrocarbon-rich gas stream.

Abstract: The invention provides a maraging steel production method in which an oxide is added during an Mg oxide formation step, the production method including: the Mg oxide formation step in which Mg is added to molten steel and MgO is formed in the molten steel, during primary melting; a consumable electrode production step in which, after the Mg oxide formation step, the molten steel is solidified and a consumable electrode having residual MgO is obtained; and a vacuum arc re-melting step in which the consumable electrode is used and vacuum arc re-melting is performed.

Abstract: A quenching agent delivery apparatus is provided for delivering a quenching agent to a component to be quenched. The delivery apparatus comprises an inlet through which the quenching agent is configured to be delivered into the apparatus, a first outlet configured to deliver quenching agent in a first direction to an inner surface of the component, and a second outlet configured to deliver quenching agent in a second direction to an inner surface of the component.

Abstract: Steel compositions are provided. A steel composition may include iron; from 0.015 to 0.06 wt. % carbon; from 9 to 12 wt. % chromium; from 0.75 to 1.5 wt. % manganese; from 0.08 to 0.18 wt. % molybdenum; from 0.10 to 0.30 wt. % silicon; from 0.2 to 1.0 wt. % vanadium; from 0.05 to 1.2 wt. % niobium; and optionally, an amount of an additional precipitate forming alloying element. Methods of making the steel compositions are also provided.

Abstract: A thermal mechanical treatment method includes consolidating a powder by a severe plastic deformation process and ageing the consolidated powder at low temperature. The method may include cryomilling the powder before consolidating the powder by a severe plastic deformation process; hot isostatic pressing the consolidated powder into a dense powder before aging the consolidated powder; hot extruding the dense powder into a stock shape before aging the consolidated powder; hot-working the stock shape on a gyrating forge at a predetermined temperature before aging the consolidated powder; or heating the consolidated powder to a predetermined temperature, and maintaining the consolidated powder at the predetermined temperature for a predetermined time.

Abstract: A method is provided for producing a hot-pressed member including heating a Ni-based coated steel sheet, which includes, on a surface thereof, a Zn—Ni alloy coating layer containing 13% by mass or more of Ni, in a temperature region of an Ac3 transformation point to 1200° C.; and then hot-pressing the steel sheet.

Abstract: In the production of a non-oriented electrical stress sheet by hot rolling a steel slab containing, by mass %, C: not more than 0.005%, Si: not more than 8.0%, Mn: 0.03-3.0%, P: not more than 0.2%, S: not more than 0.005%, Al: not more than 3.0%, N: not more than 0.005%, Ni: not more than 3%, Cr: not more than 5%, Ti: not more than 0.005%, Nb: not more than 0.003%, As: not more than 0.005% and O: not more than 0.005%, subjecting to one cold rolling or two or more cold rollings interposing an intermediate annealing therebetween after conducting a hot band annealing or without conducting a hot band annealing and subjecting to a finish annealing, an average heating rate from 600° C. to 700° C. during the heating process in the finish annealing is set to not less than 50° C./sec.

Abstract: There is provided an austenitic stainless steel having a high strength and an excellent hydrogen brittleness resistance and further having an excellent machinability. The austenitic stainless steel of the present embodiment has a chemical composition including: in mass %, C: 0.10% or less; Si: 1.0% or less; Mn: 2.1 to 6.0%; P: 0.045% or less; S: 0.1% or less; Ni: 8.0 to 16.0%; Cr: 15.0 to 30.0%; Mo: 1.0 to 5.0%; N: 0.05 to 0.45%; Nb: 0 to 0.50%; and V: 0 to 0.50%, with the balance being Fe and impurities, and satisfying Formula (1). The austenitic stainless steel of the present embodiment has a grain size number of less than 8.0 and a tensile strength of 690 MPa or more. 15?12.6C+1.

Abstract: A hollow forging process for main shaft of large wind turbine generator, wherein, comprising the following steps as: the first step of cutting off the dead head and the bottom of an ingot; the second step of upsetting and punching a hole; the third step of drawing-out; and the fourth step of local upsetting, drawing-out and shaping-up. In the fourth step, the forged piece is shaped up by local upsetting and drawing-out through a turnplate. The hollow forging process for main shaft created by the invention can save the costs for enterprise to purchase large equipment and makes it possible to forge the main shaft of large wind turbine generator with a free forging oil press with a smaller size.

Abstract: A high-tensile-strength steel plate is provided with a new chemical composition design that guarantees the same properties as a 50 mm thick steel plate even in a steel plate with a thickness of 100 mm or greater, without the yield stress being affected by the plate thickness. By mass %, the chemical composition includes C: 0.02% to 0.08%, Si: 0.01% to 0.35%, Mn: 1.4% to 2.0%, P: 0.007% or less, S: 0.0035% or less, Al: 0.010% to 0.060%, Ni: 0.5% to 2.0%, Mo: 0.10% to 0.50%, Nb: 0.005% to 0.040%, Ti: 0.005% to 0.025%, B: less than 0.0003%, N: 0.002% to 0.005%, Ca: 0.0005% to 0.0050%, and O: 0.003% or less, with the components additionally satisfying a predetermined relationship.

Abstract: There is provided a high-carbon steel wire rod and a preparation method thereof. Wherein, the high-carbon steel wire rod comprising the following ingredients: C, Si, Mn, Cr, V, Ti and Fe. The high-carbon steel wire rod has satisfactory mechanical properties, including high strength with an average tensile strength of 1560 MPa as well as good plasticity with an average after-fracture shrinkage rate of 30% and an after-fracture elongation percentage larger than or equal to 9%, which can meet the performance requirements for producing 2300 MPa level pre-stressed steel strand.

Abstract: Ultrasonic probes containing a plurality of gas delivery channels are disclosed, as well as ultrasonic probes containing recessed areas near the tip of the probe. Ultrasonic devices containing these probes, and methods for molten metal degassing using these ultrasonic devices, also are disclosed.

Abstract: A hydrometallurgical process and system for the recovery of precious metals; specifically, palladium, rhodium, and platinum metals, at high purity and with limited waste and environmental fouling.

Abstract: The present invention provides a method of separating precious metals from anode sludge obtained from copper electrolysis, comprising (a) leaching the anode sludge in an aqueous sulfuric acid solution to remove leachable chlorides and to obtain a first leaching residue depleted of chlorides; (b) pressure leaching the first leaching residue to dissolve Ag and Se and to obtain a first filtrate comprising Ag and Se and a second leaching residue depleted of Ag and Se; and (c) leaching the second leaching residue with an aqueous hydrochloric acid solution to dissolve Au and PGMs to obtain a second filtrate comprising Au and PGMs and a final leaching residue.

Abstract: It is preferable that metallic sodium to be loaded to an engine valve used for an internal combustion engine such as automobile engine have high purity. However, conventionally, an organic solvent remaining in micropores on a surface of the metallic sodium have been hardly attracted attention. Provided is a method for purifying metallic sodium including steps of placing metallic sodium containing organic solvent in the micropores thereof in a melting tank which is sealed, and heating the melting tank under reduced pressure to vaporize and remove the organic solvent coating the metallic sodium.

Abstract: Disclosed herein is a novel approach to the chemical synthesis of titanium metal from a titanium oxide source material. In the approach described herein, a titanium oxide source is reacted with Mg vapor to extract a pure Ti metal. The method disclosed herein is more scalable, cheaper, faster, and safer than prior art methods.

Abstract: The present invention relates to a method for converting and separating vanadium, titanium, and iron from the vanadium-titanium-iron concentrate in one step, which includes the steps as below. (1) The vanadium-titanium-iron concentrate is mixed and roasted together with addition agent and reducing agent, and thereby vanadium-containing pig iron and vanadium enriched slag are obtained. (2) The vanadium titanium enriched slag is leached in water and filtered, and thereby vanadium-containing solution and titanium slag are obtained. The technical features of the present invention are as below. By the new process of sodium reduction coupling, a new system of low-temperature smelting multiphase reaction separation is constructed. The reduction of iron, sodiumizing of vanadium, and the melting separation process of the vanadium titanium enriched slag and the iron are achieved in one step. Three products, i.e., the vanadium-containing pig iron, the vanadium-containing solution, and the titanium slag are produced.

Abstract: An object of the present invention is to provide a method for recovering a rare earth element, including subjecting a workpiece containing at least a rare earth element and an iron group element to an oxidation treatment, then turning the treatment environment into an environment where carbon is present, and subjecting an oxidation-treated workpiece to a heat treatment, thereby separating a rare earth element in the form of an oxide from an iron group element, according to which an oxide of a rare earth element can be efficiently separated from an iron group element at low treatment cost, and also wear and damage to the treatment container can be suppressed to allow the container to be used repeatedly for a long period of time.

Abstract: The invention provides methods, compositions, and apparatuses for preventing the formation of scale in heap leach process solution distribution systems comprised of piping, spray nozzles, or emitter tubes. Solution distribution system components often become fouled by scale because of local hot spots more prone to form scale than other locations along the systems length. Positioning sensors that detect periods of high temperature stress and adjusting scale control reagent dosage to send the right amount to inhibit hot spot deposition allows for the control of scale without using wasteful excessive amounts of scale control reagents. This can vastly improve scale control performance under high temperature stress conditions while minimizing scale control reagent waste under less severe stress conditions to reduce the total operating cost of running heap leach mining operations which depend upon well-functioning solution distribution systems.

Abstract: A gas-shielded flux cored welding electrode comprises a ferrous metal sheath and a core within the sheath enclosing core ingredients. The core ingredients and sheath together comprise, in weight percentages based on the total weight of the core ingredients and the sheath: 0.25 to 1.50 manganese; 0.02 to 0.12 carbon; 0.003 to 0.02 boron; 0.2 to 1.5 silicon; 0 to 0.3 molybdenum; at least one of titanium, magnesium, and aluminum, wherein the total content of titanium, magnesium, and aluminum is 0.2 to 2.5; 3 to 12 titanium dioxide; at least one arc stabilizer, where the total content of arc stabilizers is 0.05 to 1.0; no greater than 10 of additional flux system components; remainder iron and incidental impurities.

Abstract: An iron-based soft magnetic alloy greater than 63.5 mm in width, a thickness between 13 and 20 microns and having a composition represented by the following formula: (Fe1-aMa)100-x-y-z-p-q-rCuxSiyBzM?pM?qXr wherein M is Co and/or Ni, M? is at least one element selected from the group consisting of Nb, W, Ta, Zr, Hf, Ti and Mo, M? is at least one element selected from the group consisting of V, Cr, Mn, Al, elements in the platinum group, Sc, Y, rare earth elements, Au, Zn, Sn and Re, X is at least one element selected from the group consisting of C, Ge, P, Ga, Sb, In, Be and As, and a, x, y, z, p, q and r respectively satisfy 0?a?0.5, 0.1?x?3, 0?y?30, 1?z?25, 5?y+z?30, 0.1?p?30, q?10 and r?10, the alloy being at least 50% crystalline with an average particle size of 100 nm or less. This alloy has low core loss, high permeability and low magnetostriction.

Abstract: A method of preparing a cylindrical metal member includes preparing a metallic ingot having at least one surface having a mean width with respect to roughness Sm in a range of from 100 ?m to 220 ?m; imparting a lubricant to the at least one surface of the metallic ingot; and subjecting the metallic ingot to impact pressing while the surface coated with the lubricant with respect to the metallic ingot is set as a bottom surface, to thereby mold a cylindrical metal member.

Abstract: The invention relates to a high-tensile brass alloy comprising 58-66 wt % Cu; 1.6-7 wt % Mn; 0.2-6 wt % Ni; 0.2-5.1 wt % Al; 0.1-3 wt % Si; ?1.5 wt % Fe; ?0.5 wt % Sn; ?0.5 wt % Pb; and the remainder Zn together with unavoidable impurities. Also described are a high-tensile brass product with such an alloy composition, and a method for manufacturing such a product made of a high-tensile brass alloy.

Abstract: The invention relates to equipment for cooling a metal strip (2) having a liquid coating to be solidified, wherein said strip is continuously moving. Said equipment is characterized in that each half-cooler (11, 12) is divided, over the length thereof, into at least two sections, a first section (13) and a second section (14), in the direction of the movement of the strip (2). The first section (13) is separated from the second section (14) in each half-cooler (11, 12) by a respective internal adjustment device (7, 8), making it possible to change the gas flow/pressure parameter so that the value of said gas flow/pressure parameter is different in the first section (13) from the value of said parameter in the second section (14).

Abstract: A method for removing soluble ferrous iron from a galvanizing flux solution includes circulating the flux solution through a concentration loop and injecting ozone into the concentration loop, wherein the ozone mixes with the flux solution and reacts with soluble ferrous iron to form insoluble ferric iron in the loop. Flux solution that is substantially free of insoluble ferric iron may be removed from the concentration loop through a filter medium such as a cross-flow microfilter, thereby concentrating the ferric iron in the concentration loop. The ozone may be injected through an eductor that utilizes motive force from a circulation pump, thereby reducing energy consumption and providing rapid mixing and reaction of ozone and ferrous iron.

Abstract: The invention provides a vacuum evaporation device, belongs to the field of vacuum evaporation and can solve the problem of non-uniform thickness of a coating film formed by an existing vacuum evaporation device. The vacuum evaporation device provided by the present invention comprises an evaporation chamber, at least one evaporation source device disposed in the evaporation chamber, and a baffle assembly disposed between the evaporation source device and a substrate to be evaporated. The evaporation source device is disposed within the center region of a base plate of the evaporation chamber. The baffle assembly is provided with a baffle assembly opening used for allowing evaporation substances to pass therethrough and corresponding to the position of the evaporation source device. As the evaporation source device is disposed within the center region of the evaporation chamber, the thickness of a coating film formed on the surface of the substrate is more uniform.

Abstract: A crucible, a vacuum evaporation device and a vacuum evaporation system are disclosed. In one embodiment, the crucible includes a crucible body for accommodating an evaporation material therein, a nozzle disposed at a mouth of the crucible body, and a holding groove formed at the outer circumference of the nozzle and configured for accommodating the evaporation material overflowed from the nozzle therein, so that, the overflowed evaporation material (especially aluminum material) is prevented from falling into an interior of a heating source, avoiding damage to a heating element due to a short circuit, and meanwhile, condition of overflow of the evaporation material will be checked easily by observing the holding groove. The crucible according to some embodiments adopts a structural design with a large bottom and small mouth, improving heating area and evaporation rate of the evaporation material.

Abstract: A method for open-air pulsed laser deposition by providing a target and a substrate, configuring a laser directed to the target, reducing the pressure in the zone between the target and substrate by providing a suction having an opening proximal to the target. Optionally, shielding the zone between the target and substrate from ambient oxygen by flowing an inert gas from outside the zone. The method may accommodate very large substrates and multiple targets and multiple laser beams. The target may be tilted or remotely tilted. Matrix assisted pulsed laser deposition may be utilized.

Abstract: A sputtering target including an oxide with a low impurity concentration is provided. Provided is a method for manufacturing a sputtering target, including a first step of preparing a mixture including indium, zinc, an element M (the element M is aluminum, gallium, yttrium, or tin), and oxygen; a second step of raising a temperature of the mixture from a first temperature to a second temperature in a first atmosphere containing nitrogen at a concentration of higher than or equal to 90 vol % and lower than or equal to 100 vol %; and a third step of lowering the temperature of the mixture from the second temperature to a third temperature in a second atmosphere containing oxygen at a concentration of higher than or equal to 10 vol % and lower than or equal to 100 vol %.

Abstract: The disclosed embodiments include a system and method for manufacturing an integrated computational element (ICE) core. The method comprises varying a distance between a thermal component relative to a substrate holder that holds at least one substrate during a thin film deposition process to improve uniformity of the ICE core. In one embodiment, varying the distance between the thermal component relative to the substrate holder that holds at least one substrate includes moving at least a portion of the substrate holder in at least one direction relative to the thermal component and also moving the thermal component in at least one direction relative to the substrate holder during the thin film deposition process.

Abstract: Methods of forming an ALD-inhibiting layer using a layer of SAM molecules include providing a metalized substrate having a metal M and an oxide layer of the metal M. A reduction gas that includes a metal Q is used to reduce the oxide layer of the metal M, leaving a layer of form of M+MQyOx atop the metal M. The SAM molecules are provided as a vapor and form an ALD-inhibiting SAM layer on the M+MQyOx layer. Methods of performing S-ALD using the ALD-inhibiting SAM layer are also disclosed.

Abstract: Described herein are compositions and methods using same for forming a silicon-containing film or material such as without limitation a silicon oxide, silicon nitride, silicon oxynitride, a carbon-doped silicon nitride, or a carbon-doped silicon oxide film in a semiconductor deposition process, such as without limitation, a plasma enhanced atomic layer deposition of silicon-containing film.

Abstract: A delivery device, manufacturing system, and process of manufacturing are disclosed. The delivery device includes a feed tube and a chemical vapor deposition coating applied over an inner surface of the feed tube, the chemical vapor deposition coating being formed from decomposition of dimethylsilane. The manufacturing system includes the delivery device and a chamber in selective fluid communication with the delivery device. The process of manufacturing uses the manufacturing system to produce an article.

Abstract: A radical source for supplying radicals during atomic layer deposition semiconductor processing operations is provided. The radical source may include a remote volume, a baffle volume, and a baffle that partitions the remote volume from the baffle volume. The baffle volume and the remote volume may be fluidly connected through the baffle via a plurality of baffle holes. The baffle may be offset from a faceplate with a plurality of first gas distribution holes fluidly connected with the baffle volume. A baffle gas inlet may be fluidly connected with the baffle volume, and a first process gas inlet may be fluidly connected with the remote volume. Baffle gas may be flowed into the baffle volume to prevent radicalized first process gas in the remote volume from flowing through the baffle volume and the faceplate.