Abstract: Small scale morphology materials transport particles and viruses from within the material to a living or non-living surface or artificial representation thereof. These materials are polymer composite and have features or are measured in their entirety to be less than 100 microns.

Abstract: The present disclosure relates to systems and methods for purifying nucleic acid. In particular, the present disclosure relates to systems and methods for purifying nucleic acids using metal or metal oxide compositions.

Abstract: Provided herein are methods for immune repertoire sequencing and single cell barcoding. In some aspects, such methods may comprise producing a plurality of cDNAs from a plurality of polynucleotides, wherein at least one polynucleotide encodes a heavy and/or light chain, from a plurality of immune cells from a biological sample. Producing a plurality of cDNAs may comprise reverse transcription and template switching, such as to form a plurality of uniquely barcoded cDNAs. Methods described herein further comprise amplifying the plurality of uniquely barcoded cDNAs, thereby forming a library, and sequencing one or more of the sequences of the library.

Abstract: Provided are constructs and methods for RNA promoter identification.

Abstract: The disclosure describes novel systems, methods, and compositions for the manipulation of nucleic acids in a targeted fashion. The disclosure describes non-naturally occurring, engineered CRISPR systems, components, and methods for targeted modification of a nucleic acid.

Abstract: The present invention provides miR-122* as a functional microRNA molecule, compositions comprising the same and uses thereof for treatment of various conditions, such as, cancer. Further provided are methods for increasing the expression, stability and/or activity of p53 in a target cell or method for inducing cancer cell death, the methods comprising introducing into the cell a miR-122* polynucleotide molecule or a vector expressing or encoding the same.

Abstract: The present invention relates to the identification of a microRNA family, designated miR-29a-c, that is a key regulator of fibrosis in cardiac tissue. The inventors show that members of the miR-29 family are down-regulated in the heart tissue in response to stress, and are up-regulated in heart tissue of mice that are resistant to both stress and fibrosis. Also provided are methods of modulating expression and activity of the miR-29 family of miRNAs as a treatment for fibrotic disease, including cardiac hypertrophy, skeletal muscle fibrosis other fibrosis related diseases and collagen loss-related disease.

Abstract: Compositions and method for down modulating target gene expression which RNA interference, as well as methods for administering said compositions are disclosed. The method comprises administering a first oligonucleotide strand to a cell, incubating the cells for a time period suitable for uptake of the first oligo nucleotide strand prior to administration of a second oligonucleotide strand, wherein the first strand and the second strand form an intracellular duplex which is effective to catalyze degradation of gene target mRNA or inhibit translation of said mRNA.

Abstract: This disclosure concerns nucleic acid molecules and methods of use thereof for control of pathogens through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in pathogens. The disclosure also concerns methods for applying dsRNA through formulations and/or transgenic plants that express nucleic acid molecules useful for the control of pathogens, and the plant cells and plants obtained thereby.

Abstract: Described herein are compositions comprising a first aptamer, second aptamer and a target that are capable of forming a ternary complex, and wherein the first aptamer and the second aptamer comprise C-5 pyrimidine modification schemes that are different, and methods of making and using such compositions.

Abstract: A self-regulating gene expression construct comprises a single promoter in operative association with a repressor sequence (e.g., bacterial repressor lacI or gaiR), operator sequence(s) responsive to the expressed repressor protein, and a transgene. A dual-regulating construct comprises a single promoter controlling expression of a bacterial repressor sequence and a transgene, and which, in the presence of a first inducer molecule, transcribes the transgene and repressor; and a ribozyme in association with an aptamer sequence, the aptamer sequence capable of interacting with a second inducer molecule to terminate mRNA degradation by the ribozyme. Also provided are recombinant vectors or viruses containing the self-regulating or dual self-regulating constructs and cells containing the vectors. Such compositions are useful in methods of treating a diseases using gene therapy.

Abstract: The present invention relates to a new L-arabinose assimilation pathway and uses thereof. In particular, the present invention relates to polypeptides exhibiting L-arabinose isomerase, L-ribulokinase or L-ribulose-5-phosphate-4-epimerase activity, and recombinant host cells expressing said polypeptides. The present invention also relates to a method of producing a fermentation product, preferably ethanol, from an arabinose containing substrate, using a polypeptide or a host cell of the invention.

Abstract: A gene engineering yeast having a saccharification function, a method of preparing same, and an application of same, a nucleotide sequence for encoding glucose amylase, where: (a) the nucleotide sequence is an amino acid sequence shown by the code SEQ ID NO:16; or (b) the sequence of the encoding region of the glucose amylase of the nucleotide sequence and the nucleotide sequence shown by SEQ ID NO:15 have a similarity ?80%.

Abstract: The present invention provides genetically modified Pichia strains wherein at least one nucleic acid sequence encoding a functional gene product and/or at least one nucleic acid sequence necessary for expression of at least one functional gene product in said Pichia strain is genetically modified, wherein said gene product is responsible for proteolysis and/or glycosylation in said genetically modified Pichia strain. In particular, Pichia strains are provided wherein nucleic acid sequence encoding a functional gene product or expression of said gene product are genetically modified: PEP4, PRB1, YPS1, YPS2, YMP1, YMP2, YMP3 and PMT4.

Abstract: The disclosure provides DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The disclosure also provides nucleic acid sequences from Arabidopsis thaliana comprising novel regulatory elements identified from the AtHB17 gene. The disclosure also provides transgenic plants, plant cells, plant parts, and seeds comprising the DNA molecules operably linked to heterologous transcribable polynucleotides are also provided, as are methods of their use.

Abstract: In one aspect, the present invention provides an expression cassette comprising a promoter operably linked to a nucleic acid molecule of interest, which is operably linked to a terminator, further comprising a heterologous intron that enhances expression of the nucleic acid molecule of interest, wherein the heterologous intron is selected from the group comprised of SEQUENCE ID NO. 1 (iZm10430-02), SEQUENCE ID NO. 2 (iZm10430-01) SEQUENCE ID NO. 3 (iZm008975-01), SEQUENCE ID NO. 4 (iZm005854-01), SEQUENCE ID NO. 5 (iZm010719-01), and SEQUENCE ID NO. 6 (iZm007840-01). In another aspect, the present invention provides an expression cassette comprising a promoter operably linked to a nucleic acid molecule of interest, which is operably linked to a heterologous terminator that enhances expression of the nucleic acid molecule of interest, wherein the heterologous terminator is selected from the group of SEQUENCE ID NO. 7 (tZmHSP70-01), SEQUENCE ID NO. 8 (tZmUbi158-01), SEQUENCE ID NO.

Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements obtained from Brassica napus.

Abstract: As described herein, plants expressing algal Diacylglycerol Acyltransferase Type Two (DGTT) from heterologous nucleic acids can alter acyl carbon partitioning in plant vegetative tissues and increase acyl-CoA-dependent triacylglycerol synthesis, thereby increasing the lipid content of the plants' tissues.

Abstract: The invention refers to plants comprising wild-type or mutated Alopecurus PPO enzymes, and methods of obtaining such plants. The present invention further refers to a method for controlling weeds at a plant cultivation site, the method comprising the steps of providing, at said site, a plant that comprises at least one nucleic acid comprising a nucleotide sequence encoding a wild-type or a mutated Alopecurus PPO enzyme which is resistant or tolerant to a PPO-inhibiting herbicide by applying to said site an effective amount of said herbicide.

Abstract: The present invention discloses RNAi constructs derived from a Fusarium chitin synthase gene Chs3b, which has a nucleotide sequence as shown by SEQ ID NO: 1. Five distinct RNAi vectors are constructed for 5 different segments of the Chs3b gene (named Chs3b-1, 2, 3, 4, and 5, respectively), and separately transformed into Fusarium. It is found that siRiNAs in the transformed Fusarium shows a significant inhibition in fungal growth, development, and pathogenicity. The expression of the RNAi vectors against Chs3b in plants may inhibit the infection of Fusarium and improve the resistance of the transgenic plants to Fusarium head blight.

Abstract: This application relates to the fields of gene therapy and molecular biology. In accordance with the present invention, an adeno-associated virus (AAV) vector comprising an altered capsid protein is provided. More specifically, this invention provides adeno-associated viral vectors comprising protein capsid variants which accelerate vector breakdown and clearance, thereby reducing undesirable immune responses.

Abstract: Nucleic acid constructs and methods for rendering modifications to a genome are provided, wherein the modifications comprise null alleles, conditional alleles and null alleles comprising COINs. Multifunctional alleles (MFA) are provided, as well as methods for making them, which afford the ability in a single targeting to introduce an allele that can be used to generate a null allele, a conditional allele, or an allele that is a null allele and that further includes a COIN. MFAs comprise pairs of cognate recombinase recognition sites, an actuating sequence and/or a drug selection cassette, and a nucleotide sequence of interest, and a COIN, wherein upon action of a recombinase a conditional allele with a COIN is formed. In a further embodiment, action of a second recombinase forms an allele that contains only a COIN in sense orientation. In a further embodiment, action by a third recombinase forms an allele that contains only the actuating sequence in sense orientation.

Abstract: The present invention provides a method for high-efficiency production of pinoresinol by use of an H2O2 auto-scavenging enzymatic cascade. It uses eugenol as the substrate, which is relatively inexpensive and is industrially available. It uses an enzymatic cascade to remove H2O2 produced in the process of pinoresinol synthesis, thereby reducing its inhibitory effect on the enzyme activity. In addition, the present invention uses whole cells as a catalyst, which can continuously regenerate cofactors needed by the enzyme, thus eliminating the need for exogenous addition of expensive cofactors during the reaction. The yield of the present invention can reach 7.12 g/L and the conversion rate is 61.55%.

Abstract: Exemplary embodiments provided herein include genetically engineering microorganisms, such as yeast or bacteria, to produce cannabinoids by inserting genes that produce the appropriate enzymes for the metabolic production of a desired compound.

Abstract: The present invention relates to a fermenting method for enriching biomass of Thraustochytrium genus microalgae, specifically Schizochytrium sp. or Schizochytrium mangrovei, with docosahexaenoic acid (or DHA), by controlled addition of oxygen.

Abstract: The disclosure relates to a recombinant microorganism engineered to express an enzyme which catalyzes the conversion of a primary amine and an acyl thioester to a fatty amide. The disclosure further encompasses a method of producing a fatty amide by culturing the recombinant microorganism in the presence of a carbon source.

Abstract: The present invention provides a method for producing L-amino acids by fermentation using a bacterium belonging to the family Enterobacteriaceae which has been modified to overexpress a gene encoding an iron exporter, such as a fetB gene, fetA gene, fieF gene, or a combination of these genes.

Abstract: The present invention provides a process for the treatment of a composition comprising yeast cell walls comprising ?-1,3-glucans which are insoluble when extracted with water and partially soluble when extracted with DMSO, the process comprising contacting said composition with laminaripentaose-producing-?-1,3-glucanase and inactivating the laminaripentaose-producing-?-1,3-glucanase to result in a composition comprising yeast cell walls wherein the ?-1,3-glucans have an improved solubility in DMSO and the ratio of ?-glucans soluble in DMSO compared to water is greater than or equal to 2.

Abstract: Disclosed is a method for modifying starch to slow down the digestion rate of modified starch. The present invention utilizes two-stage GBE treatment to convert the long straight-chain starch molecules into highly branched molecules with compact structures so as to decrease the digestion rate of the starch. During the two-stage treatment, granular starch was first treated with GBE, the treated starch was gelatinized in boiling water, and the gelatinized starch was treated with GBE for a second time, leading to an enhanced effect of GBE modification. Compared with the one-staged GBE modification, the two-stage GBE modification can further increase the content of slowly digestible starch in the modified starch and thus decrease the digestion rate of starch.

Abstract: Enzymatic reactions are disclosed herein comprising water, glucose-1-phosphate, cellodextrin, and at least one cellodextrin phosphorylase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:2 or SEQ ID NO:6. These reactions produce a low molecular weight, insoluble cellulose with enhanced features.

Abstract: High molecular weight heparosan polymers are described, as are methods of producing and using the high molecular weight heparosan polymers.

Abstract: A biosynthetic method of making carboxyl CoA from long-chain carboxylic acid including expressing an ACS in a cellular system, feeding a long-chain carboxylic acid to the cellular system, growing the cellular system in a medium, and producing carboxyl CoA.

Abstract: Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express novel recombinant genes encoding steviol biosynthetic enzymes and UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce steviol or steviol glycosides, e.g., rubusoside or Rebaudioside A, which can be used as natural sweeteners in food products and dietary supplements.

Abstract: Disclosed is a simplified method for producing a protein hydrolysate, the method having the advantages of decreasing costs associated with energy, materials, and space as compared to that of conventional methods. A protein hydrolysate/enzyme composition is also disclosed.

Abstract: The present invention relates to a biosensor capable of measuring the total concentration of one or a plurality of amino acids with the use of a reagentless system comprising an electrode modified by hydrogel that comprises at least one enzyme that oxidizes at least one substrate that is at least one amino acid. In some embodiments, the biosensor comprises a hydrogel comprising alginate. In some embodiments, the biosensor comprises use of a thermophilic bacterial metabolic enzyme immobilized or attached to the hydrogel.

Abstract: Enzyme compositions including lactate responsive enzyme and stabilizing agent, including electrodes, sensors, and systems having the same. The stabilizing agent stabilizes the lactate responsive enzyme in a manner sufficient to increase sensitivity to lactate, such as for use in in vivo detection of lactate. In some compositions the lactate responsive enzyme is lactate oxidase and the sensor includes a heterocycle-containing polymer and a crosslinker.

Abstract: Provided are an airborne microbial measurement apparatus and a method of measuring the same. The airborne microbial measurement apparatus includes a particle separation device including a main body having a flow space in which airborne microorganism flows and a collection unit separably coupled to one side of the main body to collect the airborne microorganism, a reagent container in which a lysis reagent reacting with the airborne microorganism collected in the collection unit and a luminous material are stored, and a luminescence measurement device for measuring intensity of light emitted after the airborne microorganism reacts with the lysis reagent and the luminous material.

Abstract: A polypeptide biosensor that detects free NAD+ is disclosed. The polypeptide comprises a first fragment from an NAD+ dependent DNA ligase acetylation domain, a second fragment from the NAD+ dependent DNA ligase acetylation domain, and a fluorescent protein, wherein the fluorescent protein is positioned between the two DNA ligase acetylation domain fragments. Also disclosed are expression vectors comprising the biosensor as well as methods of using the biosensor to detect NAD+.

Abstract: Reagents, assays, methods, kits, devices, and systems for rapid measurement of cholesterol and cholesterol sub-fractions from a blood sample are provided. Total cholesterol, low density lipoprotein cholesterol, and high density lipoprotein cholesterol can be measured in a single assay using kinetic measurements, under conditions in which cholesterol sub-species are converted to a detectable product at distinct rates. The detectable product is measured at different times after assay initiation. A lipase, cholesterol esterase, cholesterol oxidase and a peroxidase may be used together to produce colored product in amounts directly proportional to the quantity of cholesterol converted. Methods for calculating very-low density lipoprotein cholesterol levels by further including triglyceride measurements are disclosed.

Abstract: A fluidic device is disclosed for processing a biological sample in order to extract nucleic acids contained in said sample and for subsequently amplifying said extracted nucleic acids, said device including a processed sample storage archive area 10 comprising an absorbent solid substrate 14 treated with at least one nucleic acid stabilizing reagent or reagent mix, said substrate allowing the generally dry and stabilized storage of said extracted and/or amplified nucleic acids, for example for long term storage of biological samples recovered forensically.

Abstract: Provided is a method for detecting small RNAs in a simple and highly accurate manner. Provided is a nucleic acid detection method including the following steps (a) to (c): (a) carrying out a reverse transcription reaction using a target RNA as a template and a reverse transcription primer having on its 5?-end side a sequence non-complementary to the target RNA to produce a reverse transcription product longer than the target RNA; (b) carrying out a nucleic acid amplification reaction using the reverse transcription product as a template and two primers to produce an amplified double-stranded DNA fragment having a single-stranded region at least at one end; and (c) hybridizing the single-stranded region of the amplified double-stranded DNA fragment to an oligonucleotide probe immobilized on a solid phase.

Abstract: Devices and methods for controlling reversible chemical reactions at solid-liquid interfaces are disclosed. In particular, the invention relates to a method of increasing reaction rates by concentrating a target molecule in a liquid phase in the region of a reactant or ligand immobilized on a solid followed by removal of the liquid phase and replacement with an immiscible phase, such as an immiscible gas or liquid to impede the reverse reaction. Devices for performing this method to increase the rates and degree of completion of kinetically limited ligand binding or nucleic acid hybridization reactions in affinity chromatography and microarray applications are also disclosed.

Abstract: The disclosure provides methods and kits involving site-specific endonuclease guided rolling circle amplification (RCA). Nucleic acid substrates, optionally generated by hybridizing a guidance primer to a single stranded nucleic acid, are cleaved with a site-specific endonuclease. In the presence of the target site, endonuclease cleavage of the substrate generates a nucleic acid having a free 3?-hydroxyl end, which is allowed to hybridize to covalently closed circular DNA probe (“take-off probe”), and initiate a rolling circle amplification (RCA) reaction. The methods and kits may be used to detect the presence of a target nucleic acid sequence, including detection of single nucleotide polymorphisms, and may be used to assess methylation status of a desired sequence, assess zygosity and/or ploidy status. The methods and kits may also be used to detect nucleic acids associated or indicative of medical conditions or pathogenic organisms.

Abstract: The invention provides methods for amplifying nucleic acids, particularly methods for reducing density-dependent GC bias and for reducing nucleic acid damage in a bridge amplification of a nucleic acid template. The invention also provides methods for evaluating the effect of reagents and/or additives on nucleic acid damage during bridge amplification of nucleic acid template strands. The methods are suited to solid phase amplification, for example, utilizing flow cells.

Abstract: Nucleic acid oligomeric sequences and in vitro nucleic acid amplification and detection methods for detecting the presence of HAV RNA sequences in samples are disclosed. Kits comprising nucleic acid oligomers for amplifying and detecting HAV nucleic acid sequences are disclosed.

Abstract: A method for determining the integrity of the genome of a sample and/or the quality of a library of DNA sequences obtained by deterministic restriction site whole genome amplification can include (a) amplifying the library of DNA sequences to produce first, second, and third PCR products each of a different size from 50 bp to 1000 bp, by PCR using at least one first primer pair, one second primer pair and one third primer pair, the primer pairs each hybridizing to a DNA sequence of the library having a length from 1000 bp to 5000 bp and corresponding to a sequence of the genome located respectively on a first, second and third chromosome arm; (b) detecting the first, second and third PCR products; (c) correlating the presence of the first, second and third PCR products with the integrity of the genome of the sample and/or the quality of the library.

Abstract: The invention relates to new methods of controlling the movement of polynucleotides through transmembrane pores. The invention also relates to new methods of characterizing target polynucleotides using helicases.

Abstract: The invention provides a novel class of cyanine dyes that are functionalized with sulfonic acid groups and a linker moiety that facilitates their conjugation to other species and substituent groups which increase the water-solubility, and optimize the optical properties of the dyes. Also provided are conjugates of the dyes, methods of using the dyes and their conjugates and kits including the dyes and their conjugates.

Abstract: A method of sequencing a polynucleotide strand can include providing the polynucleotide strand with a primer annealed thereto and a polymerase operably bound to the polynucleotide strand; successively exposing the polynucleotide strand to the flow of four different dNTPs according to a first predetermined ordering; and successively exposing the polynucleotide strand to the flow of four different dNTPs according to a second predetermined ordering, wherein the second predetermined ordering is different from the first predetermined ordering.

Abstract: Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.