Abstract: The present technology is generally directed to methods of increasing coal processing rates for coke ovens. In various embodiments, the present technology is applied to methods of coking relatively small coal charges over relatively short time periods, resulting in an increase in coal processing rate. In some embodiments, a coal charging system includes a charging head having opposing wings that extend outwardly and forwardly from the charging head, leaving an open pathway through which coal may be directed toward side edges of the coal bed. In other embodiments, an extrusion plate is positioned on a rearward face of the charging head and oriented to engage and compress coal as the coal is charged along a length of the coking oven. In other embodiments, a false door system includes a false door that is vertically oriented to maximize an amount of coal being charged into the oven.

Abstract: An integrated hydrotreating and steam pyrolysis process for the direct processing of a crude oil to produce olefinic and aromatic petrochemicals by separating the crude oil into light components and heavy components.

Abstract: A method for installing a device into a crude oil service operation, the method may include installing the device into a section of the crude oil service operation, wherein the device comprises a surface comprising a bonded layer coating, and may also include contacting the surface with the contaminant, wherein the contaminant is selected from the group consisting of paraffins and asphaltenes, and wherein the bonded layer is a molecularly bonded layer or a covalently bonded layer. Various systems include one having a liquid environment of paraffins and asphaltene, and a surface residing within the environment comprising a bonded layer composition. Systems also include pipelines and vessels having an internal surface therein comprising a bonded layer composition, and with hydrocarbon liquids present in the pipeline or vessel.

Abstract: Systems and methods are provided for integrating a fluidized coking process with a catalyst-enhanced coke gasification process. The catalyst for the gasification process can correspond to calcium oxide, a thermally decomposable calcium salt, a potassium salt such as potassium carbonate, or a combination thereof. Examples of suitable calcium salts can include, but are not limited to, calcium hydroxide, calcium nitrate, and calcium carbonate. The calcium oxide, potassium salts, and/or thermally decomposable calcium salts can be introduced into the integrated system, for example, as part of the feed into the coker. It has been unexpectedly discovered that using catalytic gasification as part of an integrated fluidized coking and gasification process can result in an overhead gas stream from the gasifier with increased energy content and/or overhead gas pressure.

Abstract: Systems and methods are provided for adding a heated stream of light hydrocarbons to a fluidized coking environment to improve liquid product yield and/or reduce coke production. The light hydrocarbons can correspond to C1-C10 hydrocarbons and/or hydrogen. The light hydrocarbons can be heated so that the light hydrocarbons are exposed to an activation temperature of 535° C. to 950° C. and/or an activation temperature higher than the temperature in the coking zone by 50° C. or more for an activation time prior to entering the fluidized coking reactor and/or the coking zone in the fluidized coking environment.

Abstract: Provided are a lubricant that is highly resistant to contamination and highly thermally stable, a lubricant that is highly durable and highly heat resistant, and a magnetic disk. A fluoropolyether compound contains perfluoropolyether groups, end groups each containing at least one hydroxyl group, and a linking group that is comprised of a C4-C14 hydrocarbon group and that contains at least one hydroxyl group.

Abstract: A lubricant composition comprising: (i) silver or gold nanoparticles, each of which is encapsulated by a layer of alkylthiol or alkylamine molecules; (ii) palladium or platinum nanoparticles, each of which is encapsulated by a layer of alkylthiol or alkylamine molecules; and (iii) a fluid in which components (i) and (ii) are present. Further described are methods for applying the lubricant composition onto a mechanical device for which lubrication is beneficial, with resulting improvement in friction and wear reduction and/or corrosion inhibition.

Abstract: The present invention generally relates to lubricating oil compositions useful for vehicles such as industrial machinery, especially transport, construction, and agricultural machinery. Also disclosed is a method for maintaining friction in said vehicles comprising lubricating said vehicles with said lubricating oil compositions.

Abstract: The present disclosure relates to lubricating oil compositions, and in particular, lubricating oil compositions for slideway applications, additive compositions for the slideway lubricants, and methods of lubricating a machine part, such as a slideway with the lubricating oil compositions. In one aspect, the lubricating oil compositions have a composition that provides both good friction performance and good separability from metal working fluids at the same time.

Abstract: The present invention relates to lubricant compositions for scooter engines and methods for reducing static friction between ferrous and non-ferrous surfaces as may be found in a wet clutch. The lubricant compositions contain polyether compounds in combination with one or more ash-free friction modifiers.

Abstract: Anti-microbial compositions for cleaning and sanitizing skin are disclosed. The anti-microbial compositions preferably comprises at least one quaternary ammonium chloride active and results in low irritation cleansing and sanitizing products that allow more frequent application.

Abstract: The need for an acidic hard surface cleaning composition which provides further improvements in the maintenance of surface shine, especially on inclined hard surfaces, while also using less thickener is met by formulating the acidic hard surface cleaning composition using a combination of a surface modification polymer and sulfonate hydrotrope.

Abstract: Systems and methods of forming and analyzing a dissolvable article are provided herein. In an embodiment, a method of forming a dissolvable article with excellent dissolution performance includes heating a dissolvable composition that includes a water-soluble block copolymer at heating conditions that are sufficient to render the copolymer flowable and to form a hot melt composition. The water-soluble block copolymer is solid at ambient conditions. The method further includes solidifying the dissolvable composition to form the dissolvable article. A surface of the dissolvable article is analyzed for surface texture using a three-dimensional imaging device to produce a data set that is representative of the surface texture. If the data set fails to at least equal a predetermined threshold value, the method further includes reformulating the dissolvable composition to form a reformulated dissolvable composition.

Abstract: A detergent composition comprising an enzyme and a bleaching compound and comprising two or more distinct regions, wherein the enzyme is located in a first distinct region and the bleaching compound is located in a second distinct region and further wherein a builder comprising an amino acid based compound and/or succinate based compound is located in the first distinct region. The compositions exhibit improved stability of the enzyme.

Abstract: Methods, compositions, and kit(s) for removing corrosion or scale from a metal or a non-metal article are disclosed. In a first stage of the method, an article exhibiting corrosion can be exposed to an acid wash composition including a first acid component having one or more of HCl, Phosphoric Acid, Oxalic Acid, a second acid inhibitor/surfactant component including ethoxylated alkyl mercaptan, and a third thixotropic gelling agent component including Talc or Fumed silica, sufficient to cause gelling of the acid wash composition. In a second stage of the method performed subsequent to the first stage, the article can be exposed to an acid neutralizing composition comprising bicarbonate, borax, isopropyl alcohol and water.

Abstract: An object of the present invention is to provide a solvent and a detergent that have excellent basic properties as solvents or detergents, such as solubility, and that have a boiling point, flammability, toxicity, GWP, and ODP that are all within preferable ranges. To achieve the above object, the present invention provides a solvent composition or detergent composition comprising a hydrochlorofluoropropene represented by the formula: CX3CX?CX2, wherein each X is the same or different and is F or Cl, at least one X is F, and at least three X are Cl; the hydrochlorofluoropropene having an atmospheric pressure boiling point of 50° C. or higher.

Abstract: The need for an acidic hard surface cleaning composition which provides further improvements in the maintenance of surface shine, especially the prevention of water marks and splash marks, is met by formulating the hard surface cleaning combination with a combination of surface modification polymer and crystal growth inhibiting polymer.

Abstract: An improved composition and method of use is provided which is suitable for treating live animal growth facility, meat processing facilities and food processing facilities. The composition comprises 34-99.35 wt % water; 0.1 to 3 wt % hydrophobizing agents; up to 5 wt % antistatic agents; 0.05 to 3 wt % wetting agents; and 0.5 to 55 wt % film forming agent. The composition is applied to surfaces and allowed to dry.

Abstract: The invention relates to an aqueous washing liquor in a device for cleaning soiled textile substrates, containing a plurality of water-insoluble solid particles and a liquid phase which contains a microemulsion, and to a textile washing method using said type of washing liquor.

Abstract: The present disclosure discloses a method for screening malt for brewing red beer, and belongs to the field of beer brewing. The method takes different kinds of malt samples as examples, and after whole malt mashing and dilution, mashed wort is diluted with water to a chroma value of 50-80 EBC; and when the red intensity of the mashed wort having a chromatic value of 50-80 EBC is 0.209-0.289, the measured malt can be fermented and brewed into red beer. The method can screen malt for red beer brewing simply and quickly, is of important practical significance for guiding the production of red beer, and lays an important foundation for further brewing red beer with different malt.

Abstract: The present invention relates to an enzymatic method for removing sulphite. In particular, the present invention relates to a method for converting sulphite present in a composition into thiosulphate, comprising the introduction of 3-mercaptopyruvate sulphurtransferase (3-MST) and a substrate of said 3-mercaptopyruvate sulphurtransferase into said composition. The present invention can be used in particular in the pharmaceutical, cosmetic, food and chemical fields, and any field involving the use of sulphite.

Abstract: An automated cell culture system includes a cell culture reactor including a housing; a fluidic circuit for cell culture media, the fluidic circuit disposed in an interior of the housing. The fluidic circuit includes a culture vessel for culturing cells in the cell culture media, a reservoir for the cell culture media, the reservoir fluidically connected to the culture vessel, and a pump configured to pump the cell culture media in the fluidic circuit. The automated cell culture system includes one or more sensors disposed in the interior of the housing, each sensor configured to detect a parameter of one or more of (1) the cell culture media in the fluidic circuit and (2) an environment in the interior of the housing; and a computing device configured to automatically control operation of the cell culture reactor based on one or more of the detected parameters.

Abstract: System and method includes a body having a central microchannel separated by one or more porous membranes. The membranes are configured to divide the central microchannel into a two or more parallel central microchannels, wherein one or more first fluids are applied through the first central microchannel and one or more second fluids are applied through the second or more central microchannels. The surfaces of each porous membrane can be coated with cell adhesive molecules to support the attachment of cells and promote their organization into tissues on the upper and lower surface of the membrane. The pores may be large enough to only permit exchange of gases and small chemicals, or to permit migration and transchannel passage of large proteins and whole living cells. Fluid pressure, flow and channel geometry also may be varied to apply a desired mechanical force to one or both tissue layers.

Abstract: Embodiments of systems, methods, and compositions provided herein relate to hollow beads encapsulating single cells. Some embodiments include performing multiple co-assays on a single cell encapsulated within a hollow bead, including nucleic acid sequencing, preparing nucleic acid libraries, determining methylation status, identifying genomic variants, or protein analysis.

Abstract: Reactors, systems and processes for the production of biomass by culturing microorganisms in aqueous liquid culture medium circulating in a loop reactor which utilize substantially vertical flow zones are described. Recovery and processing of the culture microorganisms to obtain products, such as proteins or hydrocarbons is described.

Abstract: A cell observation device is cell observation device for observing a cell held by a microplate having a well holding a sample including the cell and includes a microplate holder for holding the microplate thereon, an electrical stimulation unit including an electrode pair including a first electrode and a second electrode, and a position controller for controlling a position of the electrical stimulation unit in a state in which the first electrode is disposed closer to the center of the well than the second electrode when the electrode pair is disposed in the well of the microplate. The tip of the first electrode extends more than the tip of the second electrode.

Abstract: In order to perform a process of emitting a laser beam toward a cell culture vessel while maintaining the atmosphere around the cell culture vessel in a desired condition, a culture vessel housing apparatus 1 is configured to include surrounding walls 312 and 322 that surround an internal space 10 in which a cell culture vessel 9 is to be housed, a transparent top panel 4 that closes an upper part of the internal space 10 surrounded by the surrounding walls 312 and 322, a heater 5 that is provided at the top panel 4 to keep the internal space 10 warm, and a transparent bottom panel 6 that closes a lower part of the internal space 10 surrounded by the surrounding walls 312 and 322 and allows a laser beam emitted toward the cell culture vessel 9 housed in the internal space 10 to pass through.

Abstract: A method for providing a large amount of cell culture medium (10), in particular for cultivating animal cells, for a large reactor system (12) by using single-use components, comprises the following sequence of steps: a) preparing a concentrated media stock solution (32), b) filling the concentrated media stock solution (32) into single-use containers (40), c) transporting the single-use containers (40) filled with media stock solution (32) to the large reactor system (12), d) aseptically connecting the single-use containers (40) to the large reactor system (12) by means of a single-use hose system (26), e) processing the media stock solution (32) by supplying ultrapure water (24) and/or additives (44), and f) supplying the processed media stock solution (32) into the large reactor system (12).

Abstract: Protein-rich nutrient supplements and animal feed supplements derived from an anaerobic bacterial process are generated through a myriad of cell rupturing and protein fractionation/purification processes. Bacterial fermentation systems and methods of obtaining one or more protein-containing portions from a fermentation process using carbon monoxide-containing gaseous substrates are provided. The invention further provides compositions of protein-rich nutrient supplements with useful applications for intake by a variety of different animals and humans.

Abstract: Systems and methods for cleaning a water dispenser by preparing a bioremediation solution with microbes, a growth medium, and water; injecting the bioremediation solution into a water supply; and flushing the water supply with the bioremediation solution.

Abstract: In some aspects, described herein are cell culture media that are useful for in vitro culture of mammalian cells. The culture media contain a variety of small organic compounds that are found in normal adult human blood. Also described are methods of using the culture media for a variety of purposes. Also described are methods of treating cancer.

Abstract: Provided are a novel medium for expressing glycoproteins by culturing cells and a method for producing glycoproteins by culturing cells in said medium. Further provided are a medium comprising uridine and N-acetyl-D-mannosamine for the use of expression of a glycoprotein by culturing cells and a method for producing glycoproteins by culturing cells in said medium.

Abstract: The present invention relates compounds for stabilizing cells and methods of their use.

Abstract: This disclosure is directed to methods for reproducibly generating substantial amounts of endothelial cells from non-vascular cells that display improved functionality and engraftability. The endothelial cells generated in accordance with the present methodology, as well as therapeutic methods utilizing these cells, are also disclosed.

Abstract: The present invention provides bone marrow derived germline stem cells and their progenitors, methods of isolation thereof, and methods of use thereof.

Abstract: Disclosed herein are neural extracellular vesicles (EVs) and methods of using these EVs in the treatment of spinal cord injury, stroke, and traumatic brain injury and neurodegenerative diseases.

Abstract: The present invention provides a nerve cell production method that involves preparing stem cells and then introducing inducing-factor RNA into the stem cells and allowing differentiation into nerve cells. The present invention also provides a nerve cell production method that involves preparing cells, introducing reprogramming-factor RNA into the cells, introducing inducing-factor RNA into the cells into which the reprogramming-factor RNA was introduced, and allowing differentiation into nerve cells.

Abstract: A method of creating skin tissue is described, particularly, an in vitro or ex vivo method for creating skin tissue. The invention extends to the use of agents that disrupt the LINC complex in a skin cell to create the skin tissue, and to using the created tissue in an assay to identify or screen anti-ageing compounds. The invention further extends to model skin tissues per se, uses thereof and to kits for creating such model skin tissues.

Abstract: A method of growing primary human epithelial cells, in particular human epithelial cells using a basal formula containing individual (a) amino acids, (b) vitamins, (c) trace elements, and (d) other organics such as linoleic acid. The basal medium may be a mixture of amino acids, vitamins, and salts that constitute the basic media that is used to culture epithelial cells over a number of population doublings, e.g., over at least one week, while maintaining a normal phenotype and exerting low stress on the cultured cells, and maintaining lineage heterogeneity.

Abstract: Provided are feeder cell lines that can be used to expand and differentiate B cells in vitro, a method for expanding B cells in vitro comprising culturing the B cells with the feeder cell line, and a method for producing monoclonal antibody in vitro comprising culturing a single B cell with the feeder cell line under sufficient conditions and for sufficient time to induce expansion and differentiation of the B cell into a B cell done secreting antibody.

Abstract: The invention includes compositions and methods to rapidly isolate and culture cells that are potent for use in adoptive immunotherapy. In one embodiment, the isolated cells of the invention are tumor infiltrating lymphocytes (TIL) that express CD137 (also known as 4-1BB and TNFSFR9).

Abstract: Provided are a method for preparing reticulocyte simulating particles and platelet simulating particles, and a reference control. The method for preparing the reticulocyte simulating particles comprises: staining mammalian anucleated red blood cells having a volume of 60-120 fL with a protein fluorescent dye activated by N-hydroxysuccinimide, and fixing the anucleated red blood cells to prepare the reticulocyte simulating particles. The platelet simulating particles are prepared from mammalian anucleated red blood cells having a volume of 2-25 fL, and the steps of preparing the platelet simulating particles are the same as that for the reticulocyte simulating particles. The preparation method comprises: using an protein fluorescent dye activated by N-hydroxysuccinimide to stain mammalian anucleated red blood cells having different volumes, so as to respectively obtain reticulocyte simulating particles and platelet simulating particles.

Abstract: The present disclosure provides a method of deriving hepatic macrophages from stem cell-derived monocytes, through the use of hepatic macrophage culture medium comprising a hepatocyte conditioned medium and a basal medium, wherein the conditioned medium is obtained through culturing hepatocytes in a serum-free culture medium in the presence of an extracellular matrix. Also disclosed is a kit used for such a method and hepatic macrophages derived using the method and uses thereof.

Abstract: Provided herein are isolated myogenic progenitor cells, methods to isolate myogenic progenitor cells and method of their use.

Abstract: A method for detecting status of biological cells is disclosed. The method includes culturing a plurality of biological cells on a working electrode of an electrochemical biosensor, changing extracellular acidity of the plurality of cultured biological cells by adding an acidic solution onto the working electrode, monitoring an electrochemical response of the plurality of cultured biological cells by monitoring a cyclic voltammetry (CV) diagram from the plurality of cultured biological cells and/or a differential pulse voltammetry (DPV) diagram from the plurality of cultured biological cells, and detecting a status of the plurality of cultured biological cells within one of three status groups including healthy cells, non-metastatic cancer cells, and metastatic cancer cells based on the monitored electrochemical response.

Abstract: The invention provides an automated system for producing induced pluripotent stem cells (iPSCs) from adult somatic cells. Further, the system is used for producing differentiated adult cells from stem cells.

Abstract: Described herein are constructs used for B-cell genomic engineering and for expression of a transgene and/or for modulation of B cell function.

Abstract: The present invention provides a method for purifying virus particles from host cells infected with virus, wherein the virus particles are treated with benzonase and polyethylene glycol and the use of said purified viruses for therapeutic compositions.

Abstract: The invention provides a recombinant adenovirus comprising two (or more) therapeutic transgenes, e.g., CD80 and CD137L. The transgenes are preferably inserted into an E1b-19K insertion site and/or an E3 insertion site.

Abstract: The present invention relates to a method of producing a polyomavirus or polyomavirus-derived virus-like particle (VLP) carrying on its surface at least one targeting molecule that binds to a cell of interest, the method comprising the step of contacting the polyomavirus or polyomavirus-derived VLP with (i) the targeting molecule, wherein the at least one targeting molecule is glycosylated with at least one glycosyl residue that is recognised by the polyomavirus or polyomavirus-derived VLP; or (ii) a first interaction molecule, wherein the first interaction molecule is glycosylated with at least one glycosyl residue that is recognised by the polyomavirus or polyomavirus-derived VLP; and the at least one targeting molecule, wherein the at least one targeting molecule is conjugated to a second interaction molecule capable of interacting with the first interaction molecule.